Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,342,424	A→C	V490V (GTT→GTG)	unknown ←	Ribonuclease G

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,342,424	0	A	C	100.0%	196.6 / NA	71	V490V (GTT→GTG)	unknown	Ribonuclease G
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (32/39);  total (32/39)

TCGGATTGACGTTTCCGCGTCATCATTTAAGATGGTCACGAGTTCAACGGGAACATTTTTCAAGCCGAAAATCATGCGGGCGCGCACGCAAAACGGGCAATGGTCGTAAACATAAAGCTTCATAAGATCTCCTTTGTTGGTTTTCACGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATAACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTCAGCCAGTAAGCCGTGGGATTCTTCGTTAATCAGATAATCGGCGACGGCGTGAGAGGCATAAACCACGAATTGTTCGCTGGCAAATACATGGTGTACGCGAATGATTTCGCGCATGATTTCATAGCACACGGTTTCCACGGTTTTCACCCGTCCACGCCCTTGGCA  >  1/1342179‑1342669                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      tCGGATTGGCGTTTCCGCGTCATCATTTAAGATGGTCACGAGTTCAACGGGAACATTTTTCAAGCCGAAAATCATGCGGGCGCGCACGCAAAACGGGCAATGGTCGTAAACATAAAGCTTCATAAGATCTCCTTTGTTGGTTTTCACGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATcaccac                                                                                                                                                                                                                                                  <  1:238040/251‑1 (MQ=255)            tttCCGCGTCATCATTTAAGATGGTCACGAGTTCAACGGGAACATTTTTCAAGCCGAAAATCATGCGGGCGCGCACGCAAAACGGGCAATGGTCGTAAACATAAAGCTTCATAAGATCTCCTTTGTTGGTTTTCACGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAAc                                                                                                                                                                                                                                        <  1:555184/250‑1 (MQ=255)            tttCCGCGTCATCATTTAAGATGGTCACGAGTTCAACGGGAACATTTTTCAAGCCGAAAATCATGCGGGCGCGCACGCAAAACGGGCAATGGTCGTAAACATAAAGCTTCATAAGATCTCCTTTGTTGGTTTTCACGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACt                                                                                                                                                                                                                                       <  1:834005/251‑1 (MQ=255)                  cgTCATCATTTAAGATGGTCACGAGTTCAACGGGAACATTTTTCAAGCCGAAAATCATGCGGGCGCGCACGCAAAACGGGCAATGGTCGTAAACATAAAGCTTCATAAGATCTCCTTTGTTGGTTTTCACGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTc                                                                                                                                                                                                                                   >  1:751265/1‑249 (MQ=255)                        catTTAAGATGGTCACGAGTTCAACGGGAACATTTTTCAAGCCGAAAATCATGCGGGCGCGCACGCAAAACGGGCAATGGTCGTAAACATAAAGCTTCATAAGATCTCCTTTGTTGGTTTTCACGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAg                                                                                                                                                                                                                              <  1:469630/248‑1 (MQ=255)                        catTTAAGATGGTCACGAGTTCAACGGGAACATTTTTCAAGCCGAAAATCATGCGGGCGCGCACGCAAAACGGGCAATGGTCGTAAACATAAAGCTTCATAAGATCTCCTTTGTTGGTTTTCACGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGtat                                                                                                                                                                                                                           >  1:131294/1‑251 (MQ=255)                        catTTAAGATGGTCACGAGTTCAACGGGAACATTTTTCAAGCCGAAAATCATGCGGGCGCGCACGCAAAACGGGCAATGGTCGTAAACATAAAGCTTCATAAGATCTCCTTTGTTGGTTTTCACGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGtat                                                                                                                                                                                                                           >  1:409850/1‑251 (MQ=255)                         atTTAAGATGGTCACGAGTTCAACGGGAACATTTTTCAAGCCGAAAATCATGCGGGCGCGCACGCAAAACGGGCAATGGTCGTAAACATAAAGCTTCATAAGATCTCCTTTGTTGGTTTTCACGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAAc                                                                                                                                                                                                                                        <  1:517358/237‑1 (MQ=255)                         atTTAAGATGGTCACGAGTTCAACGGGAACATTTTTCAAGCCGAAAATCATGCGGGCGCGCACGCAAAACGGGCAATGGTCGTAAACATAAAGCTTCATAAGATCTCCTTTGTTGGTTTTCACGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAg                                                                                                                                                                                                                              >  1:331750/1‑247 (MQ=255)                          tttAAGATGGTCACGAGTTCAACGGGAACATTTTTCAAGCCGAAAATCATGCGGGCGCGCACGCAAAACGGGCAATGGTCGTAAACATAAAGCTTCATAAGATCTCCTTTGTTGGTTTTCACGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGt                                                                                                                                                                                                                             <  1:395417/247‑1 (MQ=255)                             aaGATGGTCACGAGTTCAACGGGAACATTTTTCAAGCCGAAAATCATGCGGGCGCGCACGCAAAACGGGCAATGGTCGTAAACATAAAGCTTCATAAGATCTCCTTTGTTGGTTTTCACGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAac                                                                                                                                                                                                                      <  1:106326/251‑1 (MQ=255)                             aaGATGGTCACGAGTTCAACGGGAACATTTTTCAAGCCGAAAATCATGCGGGCGCGCACGCAAAACGGGCAATGGTCGTAAACATAAAGCTTCATAAGATCTCCTTTGTTGGTTTTCACGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAac                                                                                                                                                                                                                      <  1:415242/251‑1 (MQ=255)                             aaGATGGTCACGAGTTCAACGGGAACATTTTTCAAGCCGAAAATCATGCGGGCGCGCACGCAAAACGGGCAATGGTCGTAAACATAAAGCTTCATAAGATCTCCTTTGTTGGTTTTCACGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAac                                                                                                                                                                                                                      <  1:561870/251‑1 (MQ=255)                                aTGGTCACGAGTTCAACGGGAACATTTTTCAAGCCGAAAATCATGCGGGCGCGCACGCAAAACGGGCAATGGTCGTAAACATAAAGCTTCATAAGATCTCCTTTGTTGGTTTTCACGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAaca                                                                                                                                                                                                                     >  1:694198/1‑249 (MQ=255)                                    tCACGAGTTCAACGGGAACATTTTTCAAGCCGAAAATCATGCGGGCGCGCACGCAAAACGGGCAATGGTCGTAAACATAAAGCTTCATAAGATCTCCTTTGTTGGTTTTCACGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAg                                                                                                                                                                                                               <  1:39931/251‑1 (MQ=255)                                        gAGTTCAACGGGAACATTTTTCAAGCCGAAAATCATGCGGGCGCGCACGCAAAACGGGCAATGGTCGTAAACATAAAGCTTCATAAGATCTCCTTTGTTGGTTTTCACGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGtttt                                                                                                                                                                                                           >  1:495494/1‑251 (MQ=255)                                         aGTTCAACGGGAACATTTTTCAAGCCGAAAATCATGCGGGCGCGCACGCAAAACGGGCAATGGTCGTAAACATAAAGCTTCATAAGATCTCCTTTGTTGGTTTTCACGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAac                                                                                                                                                                                                                      >  1:741303/1‑239 (MQ=255)                                           ttCAACGGGAACATTTTTCAAGCCGAAAATCATGCGGGCGCGCACGCAAAACGGGCAATGGTCGTAAACATAAAGCTTCATAAGATCTCCTTTGTTGGTTTTCACGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAacac                                                                                                                                                                                                                    >  1:88982/1‑239 (MQ=255)                                            tCAACGGGAACATTTTTCAAGCCGAAAATCATGCGGGCGCGCACGCAAAACGGGCAATGGTCGTAAACATAAAGCTTCATAAGATCTCCTTTGTTGGTTTTCACGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACt                                                                                                                                                                                                       <  1:180547/251‑1 (MQ=255)                                                cGGGAACATTTTTCAAGCCGAAAATCATGCGGGCGCGCACGCAAAACGGGCAATGGTCGTAAACATAAAGCTTCATAAGATCTCCTTTGTTGGTTTTCACGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATcaccac                                                                                                                                                                                                                                                  <  1:305159/204‑1 (MQ=255)                                                       aTTTTTCAAGCCGAAAATCATGCGGGCGCGCACGCAAAACGGGCAATGGTCGTAAACATAAAGCTTCATAAGATCTCCTTTGTTGGTTTTCACGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGAtttgttt                                                                                                                                                                                            <  1:832876/251‑1 (MQ=255)                                                                     aaaTCATGCGGGCGCGCACGCAAAACGGGCAATGGTCGTAAACATAAAGCTTCATAAGATCTCCTTTGTTGGTTTTCACGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACtt                                                                                                                                                                              >  1:858772/1‑251 (MQ=255)                                                                                     cacgcaAAACGGGCAATGGTCGTAAACATAAAGCTTCATAAGATCTCCTTTGTTGGTTTTCACGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTCAGCCAGTaa                                                                                                                                                              <  1:134499/251‑1 (MQ=255)                                                                                       cgcaAAACGGGCAATGGTCGTAAACATAAAGCTTCATAAGATCTCCTTTGTTGGTTTTCACGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTCAGCCAGTAAg                                                                                                                                                             >  1:367086/1‑250 (MQ=255)                                                                                         caAAACGGGCAATGGTCGTAAACATAAAGCTTCATAAGATCTCCTTTGTTGGTTTTCACGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTCAGCCAGTAAGCCg                                                                                                                                                          >  1:858099/1‑251 (MQ=255)                                                                                              cGGGCAATGGTCGTAAACATAAAGCTTCATAAGATCTCCTTTGTTGGTTTTCACGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTCAGCCAGTAAGCCGTGGGa                                                                                                                                                     <  1:808652/251‑1 (MQ=255)                                                                                                ggCAATGGTCGTAAACATAAAGCTTCATAAGATCTCCTTTGTTGGTTTTCACGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTCAGCCAGTAAGCCGTGGGAt                                                                                                                                                    <  1:565180/250‑1 (MQ=255)                                                                                                      ggTCGTAAACATAAAGCTTCATAAGATCTCCTTTGTTGGTTTTCACGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAac                                                                                                                                                                                                                      <  1:507620/178‑1 (MQ=255)                                                                                                       gTCGTAAACATAAAGCTTCATAAGATCTCCTTTGTTGGTTTTCACGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACt                                                                                                                                                                                                                                       <  1:647857/160‑1 (MQ=255)                                                                                                       gTCGTAAACATAAAGCTTCATAAGATCTCCTTTGTTGGTTTTCACGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTCAGCCAGTAAGCCGTGGGATTCTTCGtt                                                                                                                                            >  1:72088/1‑251 (MQ=255)                                                                                                       gTCGTAAACATAAAGCTTCATAAGATCTCCTTTGTTGGTTTTCACGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTCAGCCAGTAAGCCGTGGGATTCTTCGtt                                                                                                                                            >  1:793699/1‑251 (MQ=255)                                                                                                               cATAAAGCTTCATAAGATCTCCTTTGTTGGTTTTCACGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTc                                                                                                                                                                       <  1:494387/216‑1 (MQ=255)                                                                                                               cATAAAGCTTCATAAGATCTCCTTTGTTGGTTTTCACGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTCAGCCAGTAAGCCGTGGGATTCTTCGTTAATCAGAt                                                                                                                                    >  1:33511/1‑251 (MQ=255)                                                                                                               cATAAAGCTTCATAAGATCTCCTTTGTTGGTTTTCACGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTCAGCCAGTAAGCCGTGGGATTCTTCGTTAATCAGAt                                                                                                                                    >  1:298797/1‑251 (MQ=255)                                                                                                                   aaGCGTCATAAGATCTCCTTTGTTGGTTTTCACGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTCAGCCAGTAAGCCGTGGGATTCTTCGTTAATCAGATAATc                                                                                                                                <  1:110744/251‑1 (MQ=255)                                                                                                                     gCTTCATAAGATCTCCTTTGTTGGTTTTCACGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATcaccac                                                                                                                                                                                                                                                  >  1:883192/1‑135 (MQ=255)                                                                                                                           tAAGATCTCCTTTGTTGGTTTTCACGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTTCCGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTCAGCCAGTAAGCCGTGGGATTCTTCGTTAACCAGATAATCGGCGACgg                                                                                                                        >  1:206967/1‑251 (MQ=255)                                                                                                                                tctcCTTTGTTGGTTTTCACGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAAc                                                                                                                                                                                                                                        <  1:239680/134‑1 (MQ=255)                                                                                                                                 ctcCTTTGTTGGTTTTCACGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGt                                                                                                                                                                                                              <  1:407961/159‑1 (MQ=255)                                                                                                                                   ccTTTGTTGGTTTTCACGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTc                                                                                                                                                                       <  1:431025/196‑1 (MQ=255)                                                                                                                                           ttgacgatgtaaaaGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTCAGCCAGTAAGCCGTGGGATTCTTCGTTAATCAGATAATCGGCGACGGCGTGAGAGGCATAAAc                                                                                                        <  1:753416/240‑1 (MQ=255)                                                                                                                                                  aCGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTCAGCCAGTAAGCCGTGGGATTCTTCGTTAATCAGATAATCGGCGACGGCGTGAGAGGCATAAACCACGAAt                                                                                                 <  1:853115/251‑1 (MQ=255)                                                                                                                                                     gAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTCAGCCAGTAAGCCGTGGGATTCTTCGTTAATCAGATAATCGGCGACGGCGTGAGAGGCATAAACCACGAATTg                                                                                               <  1:673929/250‑1 (MQ=255)                                                                                                                                                         gTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAac                                                                                                                                                                                                                      >  1:736952/1‑127 (MQ=255)                                                                                                                                                                ggATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAac                                                                                                                                                                                                                      >  1:632546/1‑120 (MQ=255)                                                                                                                                                                     ttgtcAGTGTTTTTTAATGCCTCTCCACGACATAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCGCCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTCAGCCAGTAAGCCGTGGGATTCTTCGTTAATCAGATAATCGGCGACGGCGTGAGAGGCATAAACCACGAATTGTTCGCTGGCAAATACAt                                                                              <  1:397087/247‑1 (MQ=255)                                                                                                                                                                           gtgtTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTCAGCCAGTAAGCCGTGGGATTCTTCGTTAATCAGATAATCGGCGACGGCGTGAGAGGCATAAACCACGAATTGTTCGCTGGCAAATACATGGTGTa                                                                        >  1:546688/1‑251 (MQ=255)                                                                                                                                                                             gtTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTCAGCCAGTAAGCCGTGGGATTCTTCGTTAATCAGATAATCGGCGACGGCGTGAGAGGCATAAACCACGAATTGTTCGCTGGCAAATACATGGTGTAcg                                                                      >  1:298225/1‑251 (MQ=255)                                                                                                                                                                               tttttAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTCAGCCAGTAAGCCGTGGGATTCTTCGTTAATCAGATAATCGGCGACGGCGTGAGAGGCATAAACCACGAATTGTTCGCTGGCAAATACATGGTGTAcgcg                                                                    <  1:723694/251‑1 (MQ=255)                                                                                                                                                                               tttttAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTCAGCCAGTAAGCCGTGGGATTCTTCGTTAATCAGATAATCGGCGACGGCGTGAGAGGCATAAACCACGAATTGTTCGCTGGCAAATACATGGTGTAcgcg                                                                    <  1:601091/251‑1 (MQ=255)                                                                                                                                                                               tttttAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTCAGCCAGTAAGCCGTGGGATTCTTCGTTAATCAGATAATCGGCGACGGCGTGAGAGGCATAAACCACGAATTGTTCGCTGGCAAATACATGGTGTAcgcg                                                                    <  1:617363/251‑1 (MQ=255)                                                                                                                                                                               tttttAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTCAGCCAGTAAGCCGTGGGATTCTTCGTTAATCAGATAATCGGCGACGGCGTGAGAGGCATAAACCACGAATTGTTCGCTGGCAAATACATGGTGTAcgcg                                                                    <  1:268881/251‑1 (MQ=255)                                                                                                                                                                                ttttAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTCAGCCAGTAAGCCGTGGGATTCTTCGTTAATCAGATAATCGGCGACGGCGTGAGAGGCAt                                                                                                            >  1:234990/1‑210 (MQ=255)                                                                                                                                                                                ttttAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTCAGCCAGTAAGCCGTGGGATTCTTCGTTAATCAGATAATCGGCGACGGCGTGAGAGGCATAAACCACGAATTGTTCGCTGGCAAATACATGGTGTACGCGa                                                                   >  1:116472/1‑251 (MQ=255)                                                                                                                                                                                           ctcCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTCAGCCAGTAAGCCGTGGGATTCTTCGTTAATCAGATAATCGGCGACGGCGTGAGAGGCATAAACCACGAATTGTTCGc                                                                                          >  1:425566/1‑217 (MQ=255)                                                                                                                                                                                           ctcCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTCAGCCAGTAAGCCGTGGGATTCTTCGTTAATCAGATAATCGGCGACGGCGTGAGAGGCATAAACCACGAATTGTTCGCTGGCAAATACATGGTGTACGCGAATGATTTcgcg                                                        >  1:266511/1‑251 (MQ=255)                                                                                                                                                                                                   ccTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTCAGCCAGTAAGCCGTGGGATTCTTCGTTAATCAGATAATCGGCGACGGCGTGAGAGGCATAAACCACGAATTGTTCGCTGGCAAATACATGGTGTAcgcg                                                                    >  1:863596/1‑231 (MQ=255)                                                                                                                                                                                                        aaaCGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTCAGCCAGTAAGCCGTGGGATTCTTCGTTAATCAGATAATCGGCGACGGCGTGAGAGGCATAAACCACGAATTGTTCGCTGGCAAATACATGGTGTACGCGAATGATTTCGCGCATGATTTCATAg                                           <  1:822292/251‑1 (MQ=255)                                                                                                                                                                                                         aaCGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATAAACACTTCCAGTTCAGCCAGTAAGCCGTGGGATTCTTCGTTAATCAGATAATCGGCGACGGCGTGAGAGGCATAAACCACGAATTGTTCGCTGGCAAATACATGGTGTACGCGAATGATTTCGCGCATGATTTCATAGc                                          <  1:602041/251‑1 (MQ=255)                                                                                                                                                                                                              cGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGtt                                                                                                                                                                        <  1:72011/120‑1 (MQ=255)                                                                                                                                                                                                                     aTCCACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAACACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTCAGCCAGTAAGCCGTGGGATTCTTCGTTAATCAGATAATCGGCGACGGCGTGAGAGGCATAAACCACGAATTGTTCGCTGGCAAATACATGGTGTACGCGAATGATTTCGCGCATGATTTCATAg                                           >  1:520490/1‑238 (MQ=255)                                                                                                                                                                                                                        cACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTCAGCCAGTAAGCCGTGGGATTCTTCGTTAATCAGATAATCGGCGACGGCGTGAGAGGCATAAACCACGAATTGTTCGCTGGCAAATACATGGTGTACGCGAATGATTTCGCGCATGATTTCATAGCACACGGTTTCCACgg                           <  1:105203/251‑1 (MQ=255)                                                                                                                                                                                                                        cACCGCACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTCAGCCAGTAAGCCGTGGGATTCTTCGTTAATCAGATAATCGGCGACGGCGTGAGAGGCATAAACCACGAATTGTTCGCTGGCAAATACATGGTGTACGCGAATGATTTCGCGCATGATTTCATAGCACACGGTTTCCACgg                           <  1:92088/251‑1 (MQ=255)                                                                                                                                                                                                                             cACTTTTACATCGTCAAGGCTACATCCCCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTCAGCCAGTAAGCCGTGGGATTCTTCGTTAATCAGATAATCGGCGACGGCGTGAGAGGCATAAACCACGAATTGTTCGCTGGCAAATACATGGTGTACGCGAATGATTTCGCGCATGATTTCATAGcac                                        <  1:327116/233‑1 (MQ=255)                                                                                                                                                                                                                             cACTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTCAGCCAGTAAGCCGTGGGATTCTTCGTTAATCAGATAATc                                                                                                                                <  1:723184/145‑1 (MQ=255)                                                                                                                                                                                                                               cTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTCAGCCAGTAAGCCg                                                                                                                                                          >  1:522490/1‑117 (MQ=255)                                                                                                                                                                                                                               cTTTTACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTCAGCCAGTAAGCCGTGGGATTCTTCGTTAATCAGATAATCGGCGACGGCGTGAGAGGCATAAACCACGAATTGTTCGCTGACAAATACATGGTGTACGCGAATGATTTCGCGCATGATTTCATAGCACACGGTTTCCACGGTTTTCa                     >  1:594483/1‑250 (MQ=255)                                                                                                                                                                                                                                ttttACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTCGCCGATGAACACTTCCAGTTCAGCCAGTAAGCCGTGGGATTCTTCGTTAATCAGATAATCGGTGACGGCGTGAGAGGCATAAACCACGAATTGTTCGCTGGCAAATACATGGTGTACGCGAATGATTTCGCGCATGATTTCATAGCACACGGTTTCCACGGTTTTCAcc                   <  1:438192/251‑1 (MQ=255)                                                                                                                                                                                                                                  ttACATCGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTCAGCCAGTAAGCCGTGGGATTCTTCGTTAATCAGATAATCGGCGACGGCGTGAGAGGCATAAACCACGAATTGTTCGCTGGCAAATACATGGTGTACGCGAATGATTTCGCGCATGATTTCATAGCACACGGTTTCCACGGTTTTCACCCg                 >  1:322011/1‑251 (MQ=255)                                                                                                                                                                                                                                        cGTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTCAGCCAGTAAGCCGTGGGATTCTTCGTTAATCAGATAATCGGCGACGGCGTGAGAGGCATAAACCACGAAt                                                                                                 >  1:769803/1‑165 (MQ=255)                                                                                                                                                                                                                                         gTCAAGGCTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTCAGCCAGTAAGCCGTGGGATTCTTCGTTAATCAGATAATCGGCGACGGCGTGAGAGGCATAAACCACGAATTGTTCGCTGGCAAATACATGGTGTACGCGAATGATTTCGCGCATGATTTCATAGCACACGGTTTCCACGGTTTTCACCCGTCCACg           >  1:524374/1‑250 (MQ=255)                                                                                                                                                                                                                                                cTACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTCAGCCAGTAAGCCGTGGGATTCTTCGTTAATCAGATAATCGGCGACGGCGTGAGAGGCATAAACCACGAATTGTTCGCTGGCAAATACATGGTGTACGCGAATGATTTCGCGCATGATTTCATAGCACACGGTTTCCACGGTTTTCACCCGTCCACGCCCTTgg    >  1:797286/1‑250 (MQ=255)                                                                                                                                                                                                                                                 tACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTCAGCCAGTAAGCCGTGGGATTCTTCGTTAATCAGATAATCGGCGACGGCGTGAGAGGCATAAACCACGAATTGTTCGCTGGCAAATACATGGTGTACGCGAATGATTTCGCGCATGATTTCATAGCACACGGTTTCCACGGTTTTCACCCGTCCACGCCCTTGGCa  <  1:282666/251‑1 (MQ=255)                                                                                                                                                                                                                                                 tACATCACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTCAGCCAGTAAGCCGTGGGATTCTTCGTTAATCAGATAATCGGCGACGGCGTGAGAGGCATAAACCACGAATTGTTCGCTGGCAAATACATGGTGTACGCGAATGATTTCGCGCATGATTTCATAGCACACGGTTTCCACGGTTTTCACCCGTCCACGCCCTTGGCa  <  1:236664/251‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      TCGGATTGACGTTTCCGCGTCATCATTTAAGATGGTCACGAGTTCAACGGGAACATTTTTCAAGCCGAAAATCATGCGGGCGCGCACGCAAAACGGGCAATGGTCGTAAACATAAAGCTTCATAAGATCTCCTTTGTTGGTTTTCACGGAAAGTGCGGTGGATTTTGGCAGTGTTTTTTAATGCCTCTCCACGACCTAAAAACGCCGGCAAAATCCACCGCACTTTTACATCGTCAAGGCTACATAACCACCACATCAAACTGTTCCTGAGTATAAAACACTTCAGTTTTTACTTGGATTTGTTTGCCGATGAACACTTCCAGTTCAGCCAGTAAGCCGTGGGATTCTTCGTTAATCAGATAATCGGCGACGGCGTGAGAGGCATAAACCACGAATTGTTCGCTGGCAAATACATGGTGTACGCGAATGATTTCGCGCATGATTTCATAGCACACGGTTTCCACGGTTTTCACCCGTCCACGCCCTTGGCA  >  1/1342179‑1342669

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑