Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,342,042	T→G	E87A (GAA→GCA)	unknown ←	Glutaredoxin 2

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,342,042	0	T	G	100.0%	129.3 / NA	47	E87A (GAA→GCA)	unknown	Glutaredoxin 2
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (16/31);  total (16/31)

GCTGCCGTTTAGTGCAGTGTCCGACTGGATTAAATGTGCAAGTTGCACTAAGTCAGGGGCGAGGCGGGCGAAATAGGTGTCGGTATTCGCAAGATTTTCGGCAAAATCACCGATGGATTTTTCTTTATTTTCGGTGAAATAATCTACCGCACTTTGGGTGGCATATTCAGGCAGCCCTAGGCGGGTAAAGCGTGGGATGACCAGATGATTTTGATAGCTGCCGACCTGCTTTAACCAGGCTTCTACTTCCGGGCGCACCGTTTCCGATAACATTTTCTCGCCATAGCGTTCATCAATATAGCGCACAATGTCCAAACTTTCCGGCATGGCTTCGCCGTTTTCTTTCACTAAAATCGGCACCACTTTTTTGCCGACCAGTCGGATTGACGTTTCCGCGTCATCATTTAAGATGGTCACGAGTTCAACGGGAACATTTTTCAAGCCGAAAATCATGCGGGCGCGCACGCAAAACGGGCAATGGTCGTAAA  >  1/1341801‑1342288                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       gCTGCCGTTTAGTGCAGTGTCCGACTGGATTAAATGTGCAAGTTGCACTAAGTCAGGGGCGAGGCGGGCGAAATAGGTGTCGGTATTCGCAAGATTTTCGGCAAAATCACTGATGGATTTTTCTTTATTTTCGGTGAAATAATCTACCGCACTTTGGGTGGCATATTCAGGCAGCCCTAGGCGGGTAAAGCGTGGGATGACCAGATGATTTTGATAGCTGCCGACCTGCTTTAACCAGGCTGCTACTTc                                                                                                                                                                                                                                                 <  1:202142/249‑1 (MQ=255) gCTGCCGTTTAGTGCAGTGTCCGACTGGATTAAATGTGCAAGTTGCACTAAGTCAGGGGCGAGGCGGGCGAAATAGGTGTCGGTATTCGCAAGATTTTCGGCAAAATCACTGATGGATTTTTCTTTATTTTCGGTGAAATAATCTACCGCACTTTGGGTGGCATATTCAGGCAGCCCTAGGCGGGTAAAGCGTGGGATGACCAGATGATTTTGATAGCTGCCGACCTGCTTTAACCAGGCTGCTACTTc                                                                                                                                                                                                                                                 <  1:651444/249‑1 (MQ=255)         ttAGTGCAGTGTCCGACTGGATTAAATGTGCAAGTTGCACTAAGTCAGGGGCGAGGCGGGCGAAATAGGGGTCGGTATTCGCAAGATTTTCGGCAAAATTACTGATGGATTTTTCTTTATTTTCGGTGAAATAATCTAACGCACTTTGGGTGGCATATTCAGGCAGCCCTAGGCGGGGAAAAAGGGGGGGGAACATATGATTTTGGTAGGTGCCGCCCCGCTTTTAACAGGCTGCTAATTCCgccggct                                                                                                                                                                                                                                         >  1:788982/1‑243 (MQ=255)               cAGTGTCCGACTGGATTAAATGTGCAAGTTGCACTAAGTCAGGGGCGAGGCGGGCGAAATAGGTGTCGGTATTCGCAAGATTTTCGGCAAAATCACTGATGGATTTTTCTTTATTTTCGGTGAAATAATCTACCGCACTTTGGGTGGCATATTCAGGCAGCCCTAGGCGGGTAAAGCGTGGGATGACCAGATGATTTTGATAGCTGCCGACCTGCTTTAACCAGGCTGCTACTTCCGGGCGCACCGTTTc                                                                                                                                                                                                                                  <  1:81857/250‑1 (MQ=255)                                                 tAAGTCAGGGGCGAGGCGGGCGAAATAGGTGTCGGTATTCGCAAGATTTTCGGCAAAATCACTGATGGATTTTTCTTTATTTTCGGTGAAATAATCTACCGCACTTTGGGTGGCATATTCAGGCAGCCCTAGGCGGGTAAAGCGTGGGATGACCAGATGATTTTGATAGCTGCCGACCTGCTTTAACCAGGCTGCTACTTCCGGGCGCACCGTTTCCGATAACATTTTCTCGCCATAGCGTTCATCAatat                                                                                                                                                                                               <  1:187749/251‑1 (MQ=255)                                                 tAAGTCAGGGGCGAGGCGGGCGAAATAGGTGTCGGTATTCGCAAGATTTTCGGCAAAATCACTGATGGATTTTTCTTTATTTTCGGTGAAATAATCTACCGCACTTTGGGTGGCATATTCAGGCAGCCCTAGGCGGGTAAAGCGTGGGATGACCAGATGATTTTGATAGCTGCCGACCTGCTTTAACCAGGCTGCTACTTCCGGGCGCACCGTTTCCGATAACATTTTCTCGCCATAGCGTTCATCAatat                                                                                                                                                                                               <  1:71018/251‑1 (MQ=255)                                                 tAAGTCAGGGGCGAGGCGGGCGAAATAGGTGTCGGTATTCGCAAGATTTTCGGCAAAATCACTGATGGATTTTTCTTTATTTTCGGTGAAATAATCTACCGCACTTTGGGTGGCATATTCAGGCAGCCCTAGGCGGGTAAAGCGTGGGATGACCAGATGATTTTGATAGCTGCCGACCTGCTTTAACCAGGCTGCTACTTCCGGGCGCACCGTTTCCGATAACATTTTCTCGCCATAGCGTTCATCAatat                                                                                                                                                                                               <  1:621607/251‑1 (MQ=255)                                                 tAAGTCAGGGGCGAGGCGGGCGAAATAGGTGTCGGTATTCGCAAGATTTTCGGCAAAATCACTGATGGATTTTTCTTTATTTTCGGTGAAATAATCTACCGCACTTTGGGTGGCATATTCAGGCAGCCCTAGGCGGGTAAAGCGTGGGATGACCAGATGATTTTGATAGCTGCCGACCTGCTTTAACCAGGCTGCTACTTCCGGGCGCACCGTTTCCGATAACATTTTCTCGCCATAGCGTTCATCAatat                                                                                                                                                                                               <  1:546129/251‑1 (MQ=255)                                                 tAAGTCAGGGGCGAGGCGGGCGAAATAGGTGTCGGTATTCGCAAGATTTTCGGCAAAATCACTGATGGATTTTTCTTTATTTTCGGTGAAATAATCTACCGCACTTTGGGTGGCATATTCAGGCAGCCCTAGGCGGGTAAAGCGTGGGATGACCAGATGATTTTGATAGCTGCCGACCTGCTTTAACCAGGCTGCTACTTCCGGGCGCACCGTTTCCGATAACATTTTCTCGCCATAGCGTTCATCAatat                                                                                                                                                                                               <  1:406673/251‑1 (MQ=255)                                                   aGTCAGGGGCGAGGCGGGCGAAATAGGTGTTGGTATTCGCAAGATTTTCGGCAAAATCACTGATGGATTTTTCTTTATTTTCGGTGAAATAATCTACCGCACTTTGGGTGGCATATTCAGGCAGCCCTAGGCGGGTAAAGCGTGGGATGACCAGATGATTTTGATAGCTGCCGACCTGCTTTAACCAGGCTGCTACTTcc                                                                                                                                                                                                                                                <  1:432528/200‑1 (MQ=255)                                                    gTCAGGGGCGAGGCGGGCGAAATAGGTGTCGGTATTCGCAAGATTTTCGGCAAAATCACTGATGGATTTTTCTTTATTTTCGGTGAAATAATCTACCGCACTTTGGGTGGCATATTCAGGCAGCCCTAGGCGGGTAAAGCGTGGGGTGACCAGATGGTTTTTATAGCTGCCCACCCGCTTTAACCAGGCTGCTACTTCCGGGCGCACCCTTTCCGATAACAATTTCTCGCCATAGCGTTCATCAAtataa                                                                                                                                                                                             >  1:860204/1‑249 (MQ=255)                                                    gTCAGGGGCGAGGCGGGCGAAATAGGTGTCGGTATTCGCAAGATTTTCGGCAAAATCACTGATGGATTTTTCTTTATTTTCGGTGAAATAATCTACCGCACTTTGGGTGGCATATTCAGGCAGCCCTAGGCGGGTAAAGCGTGGGGTGACCAGATGATTTTGATAGCTGCCCACCTGCCTTAACCCGGCCGCTACTTTCGGGCGCAACGTTTCCGATAACATTTTTTCGCCCTAGCGTTCATCCATATAg                                                                                                                                                                                             >  1:165052/1‑250 (MQ=255)                                                    gTCAGGGGCGAGGCGGGCGAAATAGGTGTCGGTATTCGCAAGATTTTCGGCAAAATCACTGATGGATTTTTCTTTATTTTCGGTGAAATAATCTACCGCACTTTGGGTGGCATATTCAGGCAGCCCTAGGCGGGGAAAGCGGGGGATGACCAGATGATTTTGATAGCTGCCGACCTGCTTTAACCAGGCTGCTACTTCCGGGCGCACCGTTTCCGATAACATTTTCTCGCCATAGCGGTCATCAATATAg                                                                                                                                                                                             >  1:347739/1‑250 (MQ=255)                                                    gTCAGGGGCGAGGCGGGCGAAATAGGTGTCGGTATTCGCAAGATTTTCGGCAAAATCACTGATGGATTTTTCTTTATTTTCGGTGAAATAATCTACCGCACTTTGGGGGGCATATTCAGGCAGCCCTAGGCGGGGAAAAGGGGGGGTGACCAGATGATTTTTATAGCTGCCGACCTGCTTTAAACAGGCTGCTACTTTCGGGCGCCCCGTTTTCGATAACATTTTTTTGCCATAGCGTTTATCAATATAg                                                                                                                                                                                             >  1:535100/1‑250 (MQ=255)                                                     tCAGGGGCGAGGCGGGCGAAATAGGTGTCGGTATTCGCAAGATTTTCGGCAAAATCACTGATGGATTTTTCTTTATTTTCGGTGAAATAATCTACCGCACTTTGGGTGGCATATTCAGGCAGCCCTAGGCGGGTAAAGCGTGGGATGACCAGATGATTTTGATAGCTGCCGACCTGCTTTAACCAGGCTGCTACTTCCGGGCGCACCGTTTCCGATAACATTTTCTCGCCATAGCGTTCATCAATATAg                                                                                                                                                                                             <  1:161390/249‑1 (MQ=255)                                                     tCAGGGGCGAGGCGGGCGAAATAGGTGTCGGTATTCGCAAGATTTTCGGCAAAATCACTGATGGATTTTTCTTTATTTTCGGTGAAATAATCTACCGCACTTTGGGTGGCATATTCAGGCAGCCCTAGGCGGGTAAAGCGTGGGATGACCAGATGATTTTGATAGCTGCCGACCTGCTTTAACCAGGCTGCTACTTCCGGGCGCACCGTTTCCGATAACATTTTCTCGCCATAGCGTTCATCAATATAg                                                                                                                                                                                             <  1:396492/249‑1 (MQ=255)                                                     tCAGGGGCGAGGCGGGCGAAATAGGTGTCGGTATTCGCAAGATTTTCGGCAAAATCACTGATGGATTTTTCTTTATTTTCGGTGAAATAATCTACCGCACTTTGGGTGGCATATTCAGGCAGCCCTAGGCGGGTAAAGCGTGGGATGACCAGATGATTTTGATAGCTGCCGACCTGCTTTAACCAGGCTGCTACTTCCGGGCGCACCGTTTCCGATAACATTTTCTCGCCATAGCGTTCATCAATATAg                                                                                                                                                                                             <  1:198569/249‑1 (MQ=255)                                                        ggggCGAGGCGGGCGAAATAGGTGTCGGTATTCGCAAGATTTTCGGCAAAATCACTGATGGATTTTTCTTTATTTTCGGTGAAATAATCTACCGCACTTTGGGTGGCATATTCAGGCAGCCCTAGGCGGGGAAAGCGTGGGATGACCAGATGATTTTTATAGCTGCCGACCTGCTTTAACCAGGCTGCTACTTCCGGGCGCACCGTTTCCGATAACATTTTCTCGCCCTAGCGTTCATCAATATAg                                                                                                                                                                                             >  1:293938/1‑246 (MQ=255)                                                                   ggcgAAATAGGTGTCGGTATTCGCAAGATTTTCGGCAAAATCACTGATGGATTTTTCTTTATTTTCGGTGAAATAATCTACCGCACTTTGGGTGGCATATTCAGGCAGCCCTAGGCGGGTAAAGCGTGGGATGACCAGATGATTTTGATAGCTGCCGACCTGCTTTAACCAGGCTGCTACTTCCGGGCGCACCGTTTCCGATAACATTTTCTCGCCATAGCGTTCATCAATATAg                                                                                                                                                                                             >  1:478949/1‑235 (MQ=255)                                                                     cgAAATAGGTGTCGGTATTCGCAAGATTTTCGGCAAAATCACTGATGGATTTTTCTTTATTTTCGGTGAAATAATCTACCGCACTTTGGGTGGCATATTCAGGCAGCCCTAGGCGGGTAAAGCGTGGGATGACCAGATGATTTTGATAGCTGCCGACCTGCTTTAACCAGGCTGCTACTTCCGGGCGCACCGTTTCCGATAACATTTTCTCGCCATAGCGTTCATCAATATAGCGCACAATGTCCAAACt                                                                                                                                                                            <  1:728064/250‑1 (MQ=255)                                                                       aaaTAGGTGTCGGTATTCGCAAGATTTTCGGCAAAATCACTGATGGATTTTTCTTTATTTTCGGTGAAATAATCTACCGCACTTTGGGTGGCATATTCAGGCAGCCCTAGGCGGGTAAAGCGTGGGATGACCAGATGATTTTGATAGCTGCCGACCTGCTTTAACCAGGCTGCTACTTCCGGGCGCACCGTTTCCGATAACATTTTCTCGCCATAGCGTTCATCAatat                                                                                                                                                                                               >  1:350883/1‑229 (MQ=255)                                                                             gtgtCGGTATTCGCAAGATTTTCGGCAAAATCACTGATGGATTTTTCTTTATTTTCGGTGAAATAATCTACCGCACTTTGGGTGGCATATTCAGGCAGCCCTAGGCGGGTAAAGCGTGGGATGACCAGATGATTTTGATAGCTGCCGACCTGCTTTAACCAGGCTGCTACTTCCGGGCGCACCGTTTCCGATAACATTTTCTCGCCATAGCGTTCATCAATATAGCGCACAATGTCCAAACTTTCCGGc                                                                                                                                                                     <  1:629420/249‑1 (MQ=255)                                                                                   gTATTCGCAAGATTTTCGGCAAAATCACTGATGGATTTTTCTTTATTTTCGGTGAAATAATCTACCGCACTTTGGGTGGCATATTCAGGCAGCCCTAGGCGGGTAAAGCGTGGGATGACCAGATGATTTTGATAGCTGCCGACCTGCTTTAACCAGGCTGCTACTTCCGGGCGCACCGTTTCCGATAACATTTTCTCGCCATAGCGTTCATCAatat                                                                                                                                                                                               <  1:121174/217‑1 (MQ=255)                                                                                   gTATTCGCAAGATTTTCGGCAAAATCACTGATGGATGTTTCTTTATTTTCGGTGAAATAATCTACCGCACTTTGGGTGGCATATTCAGGCAGCCCTAGGCGGGTAAAGCGTGGGATGACCAGATGATTTTGATAGCTGCCGACCTGCTTTAACCAGGCTGCTACTTCCGGGCGCACCGTTTCCGATAACATTTTCTCGCCATAGCg                                                                                                                                                                                                          <  1:452858/206‑1 (MQ=255)                                                                                       tCGCAAGATTTTCGGCAAAATCACTGATGGATTTTTCTTTATTTTCGGTGAAATAATCTACCGCACTTTGGGTGGCATATTCAGGCAGCCCTAGGCGGGTAAAGCGTGGGATGACCAGATGATTTTGATAGCTGCCGACCTGCTTTAACCAGGCTGCTACTTCCGGGCGCACCGTTTCCGATAACATTTTCTCGCCATAGCGTTCATCAATATAGCGCACAATGTCCAAACTTTCCGGCATGGCTTCGCCg                                                                                                                                                         <  1:358561/251‑1 (MQ=255)                                                                                                                                       tGAAATAATCTACCGCACTTTGGGTGGCATATTCAGGCAGCCCTAGGCGGGTAAAGCGTGGGATGACCAGATGATTTTGATAGCTGCCGACCTGCTTTAACCAGGCTGCTACTTCCGGGCGCACCGTTTCCGATAACATTTTCTCGCCATAGCGTTCATCAATATAGCGCACAATGTCCAAACTTTCCGGCATGGCTTCGCCGTTTTCTTTCACTAAAATCGGCACCACTTTTTTGCCGACCAGTCGGAt                                                                                                          <  1:254772/250‑1 (MQ=255)                                                                                                                                                cTACCGCACTTTGGGTGGCATATTCAGGCAGCCCTAGGCGGGTAAAGCGTGGGATGACCAGATGATTTTGATAGCTGCCGACCTGCTTTAACCAGGCTGCTACTTCCGGGCGCACCGTTTCCGATAACATTTTCTCGCCATAGCGTtcatc                                                                                                                                                                                                    <  1:403611/151‑1 (MQ=255)                                                                                                                                                                  cATATTCAGGCAGCCCTAGGCGGGTAAAGCGTGGGATGACCAGATGATTTTGATAGCTGCCGACCTGCTTTAACCAGGCTGCTACTTCCGGGCGCACCGTTTCCGATAACATTTTCTCGCCATAGCGTTCATCAATATAGCGCACAATGTCCAAACTTTCCGGCATGGCTTCGCCGTTTTCTTTCACTAAAATCGGCACCACTTTTTTGCCGACCAGTCGGATTGGCGTTTCCGCGTCATCATTTAAGAt                                                                               <  1:338484/250‑1 (MQ=255)                                                                                                                                                                           gCAGCCCTAGGCGGGTAAAGCGTGGGATGACCAGATGATTTTGATAGCTGCCGACCTGCTTTAACCAGGCTGCTACTTCCGGGCGCACCGTTTCCGATAACATTTTCTCGCCATAGCGTTCATCAATATAGCGCACAATGTCCAAACTTTCCGGCATGGCTTCGCCGTTTTCTTTCACTAAAATCGGCACCACTTTTTTGCCGACCAGTCGGATTGGCGTTTCCGCGTCATCATTTAAGATGGTCACGAg                                                                      <  1:474985/250‑1 (MQ=255)                                                                                                                                                                           gCAGCCCTAGGCGGGTAAAGCGTGGGATGACCAGATGATTTTGATAGCTGCCGACCTGCTTTAACCAGGCTGCTACTTCCGGGCGCACCGTTTCCGATAACATTTTCTCGCCATAGCGTTCATCAATATAGCGCACAATGTCCAAACTTTCCGGCATGGCTTCGCCGTTTTCTTTCACTAAAATCGGCACCACTTTTTTGCCGACCAGTCGGATTGGCGTTTCCGCGTCATCATTTAAGATGGTCACGAg                                                                      <  1:211888/250‑1 (MQ=255)                                                                                                                                                                              gCCCTAGGCGGGTAAAGCGTGGGATGACCAGATGATTTTGATAGCTGCCGACCTGCTTTAACCAGGCTGCTACTTCCGGGCGCACCGTTTCCGATAACATTTTCTCGCCATAGCGTTCATCAatat                                                                                                                                                                                               <  1:215560/126‑1 (MQ=255)                                                                                                                                                                              gCCCTAGGCGGGTAAAGCGTGGGATGACCAGATGATTTTGATAGCTGCCGACCTGCTTTAACCAGGCTGCTACTTCCGGGCGCACCGTTTCCGATAACATTTTCTCGCCATAGCGTTCATCAATATAGCGCACAATGTCCAAACTTTCCGGCATGGCTTCGCCGTTTTCTTTCACTAAAATCGGCACCACTTTTTTGCCGACCAGTCGGATTGGCGTTTCCGCGTCATCATTTAAGAt                                                                               <  1:844464/238‑1 (MQ=255)                                                                                                                                                                                  tAGGCGGGTAAAGCGTGGGATGACCAGATGATTTTGATAGCTGCCGACCTGCTTTAACCAGGCTGCTACTTCCGGGCGCACCGTTTCCGATAACATTTTCTCGCCATAGCGTTCATCAATATAGCGCACAATGTCCAAACTTTCCGGCATGGCTTCGCCGTTTTCTTTCACTAAAATCGGCACCACTTTTTTGCCGACCAGTCGGATTGGCGTTTCCGCGTCATCATTTAAGATGGTCACGAGTTCAACgg                                                              <  1:366682/251‑1 (MQ=255)                                                                                                                                                                                        ggTAAAGCGTGGGATGACCAGATGATTTTGATAGCTGCCGACCTGCTTTAACCAGGCTGCTACTTCCGGGCGCACCGTTTCCGATAACATTTTCTCGCCATAGCGTTCATCAATATAGCGCACAATGTCCAAACTTTCCGGCATGGCTTCGCCGTTTTCTTTCACTAAAATCGGCACCACTTTTTTGCCGACCAGTCGGATTGGCGTTTCCGCGTCATCATTTAAGATGGt                                                                            <  1:4271/231‑1 (MQ=255)                                                                                                                                                                                                         ccAGATGATTTTGATAGCTGCCGACCTGCTTTAACCAGGCTGCTACTTCCGGGCGCACCGTTTCCGATAACATTTTCTCGCCATAGCGt                                                                                                                                                                                                         <  1:745465/89‑1 (MQ=255)                                                                                                                                                                                                            gatgatTTTGATAGCTGCCGACCTGCTTTAACCAGGCTGCTACTTCCGGGCGCACCGTTTCCGATAACATTTTCTCGCCATAGCGTTCATCAATATAGCGCACAATGTCCAAACTTTCCGGCATGGCTTCGCCGTTTTCTTTCACTAAAATCGGCACCACTTTTTTGCCGACCAGTCGGATTGGCGTTTCCGCGTCATCATTTAAGATGGTCACGAGTTCAACGGGAACATTTTTCAAGCCGAAAATCATg                                    >  1:587920/1‑251 (MQ=255)                                                                                                                                                                                                               gatTTTGATAGCTGCCGACCTGCTTTAACCAGGCTGCTACTTCCGGGCGCACCGTTTCCGATAACATTTTCTCGCCATAGCGTTCATCAatat                                                                                                                                                                                               <  1:442466/93‑1 (MQ=255)                                                                                                                                                                                                                                aCCTGCTTTAACCAGGCTGCTACTTCCGGGCGCACCGTTTCCGATAACATTTTCTCGCCATAGCGTTCATCAATATAGCGCACAATGTCCAAACTTTCCGGCATGGCTTCGCCGTTTTCTTTCACTAAAATCGGCACCACTTTTTTGCCGACCAGTCGGATTGGCGTTTCCGCGTCATCATTTAAGATGGTCACGAGTTCAACGGGAACATTTTTCAAGCCGAAAATCATGCGGGCGCGCACGCAAAACg                 <  1:206529/250‑1 (MQ=255)                                                                                                                                                                                                                                    gCTTTAACCAGGCTGCTACTTCCGGGCGCACCGTTTCCGATAACATTTTCTCGCCATAGCGTTCATCAATATAGCGCACAATGTCCAAACTTTCCGGCATGGCTTCGCCGTTTTCTTTCACTAAAATCGGCACCACTTTTTTGCCGACCAGTCGGATTGGCGTTTCCGCGTCATCATTTAAGATGGTCACGAGTTCAACGGGATCATTTTTCAAGCCGAAAATCATGCGGGCGCGCACGCAAAACGGGCaa            >  1:880305/1‑251 (MQ=255)                                                                                                                                                                                                                                    gCTTTAACCAGGCTGCTACTTCCGGGCGCACCGTTTCCGATAACATTTTCTCGCCATAGCGTTCATCAATATAGCGCACAATGTCCAAACTTTCCGGCATGGCTTCGCCGTTTTCTTTCACTAAAATCGGCACCACTTTTTTGCCGACCAGTCGGATTGGCGTTTCCGCGTCATCATTTAAGATGGTCACGAGTTCAACGGGAACATTTTTCAAGCCGAAAATCATGCGGGCGCGCACGCAAAACGGGCaa            >  1:181372/1‑251 (MQ=255)                                                                                                                                                                                                                                    gCTTTAACCAGGCTGCTACTTCCGGGCGCACCGTTTCCGATAACATTTTCTCGCCATAGCGTTCATCAATATAGCGCACAATGTCCAAACTTTCCGGCATGGCTTCGCCGTTTTCTTTCACTAAAATCGGCACCACTTTTTTGCCGACCAGTCGGATTGGCGTTTCCGCGTCATCATTTAAGATGGTCACGAGTTCAACGGGAACATTTTTCAAGCCGAAAATCATGCGGGCGCGCACGCAAAACGGGCaa            >  1:28199/1‑251 (MQ=255)                                                                                                                                                                                                                                    gCTTTAACCAGGCTGCTACTTCCGGGCGCACCGTTTCCGATAACATTTTCTCGCCATAGCGTTCATCAATATAGCGCACAATGTCCAAACTTTCCGGCATGGCTTCGCCGTTTTCTTTCACTAAAATCGGCACCACTTTTTTGCCGACCAGTCGGATTGGCGTTTCCGCGTCATCATTTAAGATGGTCACGAGTTCAACGGGAACATTTTTCAAGCCGAAAATCATGCGGGCGCGCACGCAAAACGGGCaa            >  1:508548/1‑251 (MQ=255)                                                                                                                                                                                                                                        tAACCAGGCTGCTACTTCCGGGCGCACCGTTTCCGATAACATTTTCTCGCCATAGCGTTCATCAATATAGCGCACAATGTCCAAACTTTCCGGCATGGCTTCGCCGTTTTCTTTCACTAAAATCGGCACCACTTTTTTGCCGACCAGTCGGATTGGCGTTTCCGCGTCATCATTTAAGATGGTCACGAGTTCAACGGGAACATTTTTCAAGCCGAAAATCATGCGGGCGCGCACGCAAAACGGGCAATGGt        <  1:547509/251‑1 (MQ=255)                                                                                                                                                                                                                                          aCCAGGCTGCTACTTCCGGGCGCACCGTTTCCGATAACATTTTCTCGCCATAGCGTTCATCAATATAGCGCACAATGTCCAAACTTTCCGGCATGGCTTCGCCGTTTTCTTTCACTAAAATCGGCACCACTTTTTTGCCGACCAGTCGGATTGGCGTTTCCGCGTCATCATTTAAGATGGTCACGAGTTCAACGGGAACATTTTTCAAGCCGAAAATCATGCGGGCGCGCACGCAAAACGGGCAATGGTCg      <  1:92905/251‑1 (MQ=255)                                                                                                                                                                                                                                              ggCTGCTACTTCCGGGCGCACCGTTTCCGATAACATTTTCTCGCCATAGCGTTCATCAATATAGCGCACAATGTCCAAACTTTCCGGCATGGCTTCGCCGTTTTCTTTCACTAAAATCGGCACCACTTTTTTGCCGACCAGTCGGATTGGCGTTTCCGCGTCATCATTTAAGATGGTCACGAGTTCAACGGGAACATTTTTCAAGCCGAAAATCATGCGGGCGCGCACGCAAAACGGGCAATGGTCGTaaa  >  1:541069/1‑251 (MQ=255)                                                                                                                                                                                                                                              ggCTGCTACTTCCGGGCGCACCGTTTCCGATAACATTTTCTCGCCATAGCGTTCATCAATATAGCGCACAATGTCCAAACTTTCCGGCATGGCTTCGCCGTTTTCTTTCACTAAAATCGGCACCACTTTTTTGCCGACCAGTCGGATTGGCGTTTCCGCGTCATCATTTAAGATGGTCACGAGTTCAACGGGAACATTTTTCAAGCCGAAAATCATGCGGGCGCGCACGCAAAACGGGCAATGGTCGTaaa  >  1:76934/1‑251 (MQ=255)                                                                                                                                                                                                                                              ggCTGCTACTTCCGGGCGCACCGTTTCCGATAACATTTTCTCGCCATAGCGTTCATCAATATAGCGCACAATGTCCAAACTTTCCGGCATGGCTTCGCCGTTTTCTTTCACTAAAATCGGCACCACTTTTTTGCCGACCAGTCGGATTGGCGTTTCCGCGTCATCATTTAAGATGGTCACGAGTTCAACGGGAACATTTTTCAAGCCGAAAATCATGCGGGCGCGCACGCAAAACGGGCAATGGTCGTaaa  >  1:338736/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                       GCTGCCGTTTAGTGCAGTGTCCGACTGGATTAAATGTGCAAGTTGCACTAAGTCAGGGGCGAGGCGGGCGAAATAGGTGTCGGTATTCGCAAGATTTTCGGCAAAATCACCGATGGATTTTTCTTTATTTTCGGTGAAATAATCTACCGCACTTTGGGTGGCATATTCAGGCAGCCCTAGGCGGGTAAAGCGTGGGATGACCAGATGATTTTGATAGCTGCCGACCTGCTTTAACCAGGCTTCTACTTCCGGGCGCACCGTTTCCGATAACATTTTCTCGCCATAGCGTTCATCAATATAGCGCACAATGTCCAAACTTTCCGGCATGGCTTCGCCGTTTTCTTTCACTAAAATCGGCACCACTTTTTTGCCGACCAGTCGGATTGACGTTTCCGCGTCATCATTTAAGATGGTCACGAGTTCAACGGGAACATTTTTCAAGCCGAAAATCATGCGGGCGCGCACGCAAAACGGGCAATGGTCGTAAA  >  1/1341801‑1342288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑