Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,339,570	G→A	intergenic (‑256/‑98)	unknown ← / → unknown	Haemophilus‑specific protein, uncharacterized/Pyruvate/2‑oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,339,570	0	G	A	100.0%	62.5 / NA	25	intergenic (‑256/‑98)	unknown/unknown	Haemophilus‑specific protein, uncharacterized/Pyruvate/2‑oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/15);  total (10/15)

AACTCTTTCTTGAAGATGAGTTTGCCCATTTTTTGATTTAGCTTGATTTAACATATTTTCTCCAACGCGCGCGGATTGTAATAATGATCTATCTTGGAATCATACAACAAAATTGCCAAAAGGTAAAAGGCCCAAGGGAGCCCTATCGGGCTAAGGGTAAAATTTAAGGATAACCTTCCCAAGGGAAAGGTCTTACCTAAAAAGGTCAAAAGGCCTTTTCAAAAGGCTGGAACTACACTCACTATATTTGTTCGTTTTTAATGATCACTTATTTGTTGTTTTGATGTCCATCACTTGTATTCTGCAACATTAAACAGTTCCCCTTACTAAAACATGAAGCAACTATAATGTTTTCTATTTTTCATAAAATGCTAAGTAAAAAAAGTAATACCGAATCCCTTGGTATTTCTACCCAGCCAACACTGTCGGTG  >  1/1339321‑1339751                                                                                                                                                                                                                                                          |                                                                                                                                                                                      taCTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTCCAA‑‑‑‑CACGGAT‑‑‑‑CTA‑‑CA‑‑TACCTTGAAATCATACAACAAAACCGCAAAAAGGTAAAAGGAACAAGGGAGCCCTAGCGGGCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATg                                                                                                                                                                          <  1:353378/250‑1 (MQ=255)                                                                        ctccaacacggatctacatacctTGAAATCATACAACAAAACCGCAAAAAGGTAAAAGGAACAAGGGAGCCCTAGCGGGCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATGATCACTTATTTGTTGTTTTGATATTCATCACTTGTATTc                                                                                                                                   >  1:397000/22‑231 (MQ=255)                                                                        ctccaacacggatctacatacctTGAAATCATACAACAAAACCGCAAAAAGGTAAAAGGAACAAGGGAGCCCTAGCGGGCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATGATCACTTATTTGTTGTTTTGATATTCATCACTTGTATTCTGCAACATTaaat                                                                                                                      >  1:690409/22‑243 (MQ=255)                                                                                  gatctacatacctTGAAATCATACAACAAAACCGCAAAAAGGTAAAAGGAACAAGGGAGCCCTAGCGGGCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAAt                                                                                                                                                                           >  1:660186/12‑181 (MQ=255)                                                                                  gatctacatacctTGAAATCATACAACAAAACCGCAAAAAGGTAAAAGGAACAAGGGAGCCCTAGCGGGCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATg                                                                                                                                                                          <  1:792599/171‑1 (MQ=255)                                                                                  gatctacatacctTGAAATCATACAACAAAACCGCAAAAAGGTAAAAGGAACAAGGGAGCCCTAGCGGGCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATGATCACTTATTTGTTGTTTTGATATTCATCACTTGTATTCTGCAACATTaaat                                                                                                                      <  1:604511/223‑2 (MQ=255)                                                                                  gatctacatacctTGAAATCATACAACAAAACCGCAAAAAGGTAAAAGGAACAAGGGAGCCCTAGCGGGCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATGATCACTTATTTGTTGTTTTGATATTCATCACTTGTATTCTGCAACATTAAATAACACCCCTTACTaag                                                                                                      <  1:626096/239‑2 (MQ=255)                                                                                      tacatacctTGAAATCATACAACAAAACCGCAAAAAGGTAAAAGGAACAAGGGAGCCCTAGCGGGCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATGATCACTTATTTGTTGTTTTGATATTCATCACTTGTATTCTGCAACATTAAATAACACCCCTTACTaagatatg                                                                                                 >  1:541017/8‑245 (MQ=255)                                                                                       acatacctTGAAATCATACAACAAAACCGCAAAAAGGTAAAAGGAACAAGGGAGCCCTAGCGGGCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATGATCACTTATTTGTTGTTTTGATATTCATCACTTGTATTCTGCAACATTaaataac                                                                                                                   <  1:197498/226‑5 (MQ=255)                                                                                         atacctTGAAATCATACAACAAAACCGCAAAAAGGTAAAAGGAACAAGGGAGCCCTAGCGGGCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATGATCACTTATTTGTTGTTTTGATATTCATCACTTGTATTCTGCAACATTAAATAACACCCCTTACTAAGATATGAAg                                                                                              <  1:190343/247‑1 (MQ=255)                                                                                             ctTGAAATCATACAACAAAACCGCAAAAAGGTAAAAGGAACAAGGGAGCCCTAGCGGGCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATGATCACTTATTTGTTGTTTTGATATTCATCACTTGTATTCTGCAACATTAAATAACACCCCTTACTAAGATATGAAGCAAc                                                                                          <  1:360243/251‑1 (MQ=255)                                                                                                  aaTCATACAACAAAACCGCAAAAAGGTAAAAGGAACAAGGGAGCCCTAGCGGGCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATGATCACTTATTTGTTGTTTTGATATTCATCACTTGTATTCTGCAACATTAAATAACACCCCTTACTAAGATATGAAGCAACtatgt                                                                                     <  1:210347/251‑3 (MQ=255)                                                                                                    tCATACAACAAAACCGCAAAAAGGTAAAAGGAACAAGGGAGCCCTAGCGGGCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAAt                                                                                                                                                                           >  1:582822/1‑163 (MQ=255)                                                                                                         caacaaAACCGCAAAAAGGTAAAAGGAACAAGGGAGCCCTAGCGGGCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATGATCACTTATTTGTTGTTTTGATATTCATCACTTGTATTCTGCAACATTAAATAACACCCCTTACTAAGATATGAAGCAACtatgttttccat                                                                              <  1:234863/251‑10 (MQ=255)                                                                                                                                aggaacAAGGGAGCCCTAGCGGGCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATGATCACTTATTTGTTGTTTTGATATTCATCACTTGTATTCTGCAACATTAAATAACACCCCTTACTAAGATATGAAGCAACT‑‑‑ATGTTTTCCATTTTTCATAAAATCCTAAGTaaaa                                                    >  1:416088/6‑251 (MQ=255)                                                                                                                                            gCCCTAGCGGGCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATGATCACTTATTTGTTGTTTTGATATTCATCACTTGTATTCTGCAACATTaaataacaccccttacta                                                                                                        <  1:63624/190‑16 (MQ=255)                                                                                                                                               ctagcGGGCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATGATCACTTATTTGTTGTTTTGATATTCATCACTTGTATTCTGCAACATTAAATAACACCCCTTACTaag                                                                                                      <  1:572970/185‑2 (MQ=255)                                                                                                                                                 agcGGGCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATGATCACTTATTTGTTGTTTTGATATTCATCACTTGTATTCTGCAACATTAAATAACACCCCTTACTAAGATATGAAGCAACT‑‑‑ATGTTTTCCATTTTTCATAAAATCCTAAGTAAAAAATCTAATAc                                          <  1:98711/242‑1 (MQ=255)                                                                                                                                                      gCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATGATCACTTATTTGTTGTTTTGATATTCATCACTTGTATTCTGCAACATTAAATAACCCCCCTTACTAAGATATGAAGCAACT‑‑‑ATGTTTTCCATTTTTCATAAAATCCTAAGTAAAAAATCTAATACCGAATCCTTTga                              >  1:442552/1‑250 (MQ=255)                                                                                                                                                           gggTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATGATCACTTATTTGTTGTTTTGATATTCATCACTTGTATTCTGCAACATTAAATAACACCCCTTACTAAGATATGAAGCAACT‑‑‑ATGTTTTCCATTTTTCATAAAATCCTAAGTAAAAAATCTAATACCGAATCCTTTGATAtta                         >  1:131650/1‑250 (MQ=255)                                                                                                                                                                 aaTTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATGATCACTTATTTGTTGTTTTGATATTCATCACTTGTATTCTGc                                                                                                                                <  1:737542/145‑1 (MQ=255)                                                                                                                                                                 aaTTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATGATCACTTATTTGTTGTTTTGATATTCATCACTTGTATTCTGCAACATTAAATAACACCCCTTACTAAGATATGAAGCAACT‑‑‑ATGTTTTCCATTTTTCATAAAATCCTAAGTAAAAAATCTAATACCGAATCCTTTGATATTACTatcc                   <  1:839919/251‑4 (MQ=255)                                                                                                                                                                            aaCCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATGATCACTTATTTGTTGTTTTGATATTCATCAc                                                                                                                                           >  1:65838/1‑123 (MQ=255)                                                                                                                                                                                  tccAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATGATCACTTATTTGTTGTTTTGATATTCATCACTTGTATTCTGCAACATTAAATAACACCCCTTACTAAGATATGAAGCAACT‑‑‑ATGTTTTCCATTTTTCATAAAATCCTAAGTAAAAAATCTAATACCGAATCCTTTGATATTACTATCCAACCGACACtaccaatg  <  1:787157/250‑8 (MQ=255)                                                                                                                                                                                                                         tttCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATGATCACTTATTTGTTGTTTTGATATTCATCACTTGTATTc                                                                                                                                   >  1:774784/1‑86 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                      AACTCTTTCTTGAAGATGAGTTTGCCCATTTTTTGATTTAGCTTGATTTAACATATTTTCTCCAACGCGCGCGGATTGTAATAATGATCTATCTTGGAATCATACAACAAAATTGCCAAAAGGTAAAAGGCCCAAGGGAGCCCTATCGGGCTAAGGGTAAAATTTAAGGATAACCTTCCCAAGGGAAAGGTCTTACCTAAAAAGGTCAAAAGGCCTTTTCAAAAGGCTGGAACTACACTCACTATATTTGTTCGTTTTTAATGATCACTTATTTGTTGTTTTGATGTCCATCACTTGTATTCTGCAACATTAAACAGTTCCCCTTACTAAAACATGAAGCAACTATAATGTTTTCTATTTTTCATAAAATGCTAAGTAAAAAAAGTAATACCGAATCCCTTGGTATTTCTACCCAGCCAACACTGTCGGTG  >  1/1339321‑1339751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑