Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,339,554	T→A	intergenic (‑240/‑114)	unknown ← / → unknown	Haemophilus‑specific protein, uncharacterized/Pyruvate/2‑oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,339,554	0	T	A	100.0%	76.4 / NA	29	intergenic (‑240/‑114)	unknown/unknown	Haemophilus‑specific protein, uncharacterized/Pyruvate/2‑oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (13/16);  total (13/16)

AACATTGATAAAACTCTTTCTTGAAGA‑TGAGTTTGCCCATTTTTTGATTTAGCTTGATTTAACATATTTTCTCCAACGCGCGCGGATTGTAATAATGATCTATCTTGGAATCATACAACAAAATTGCCAAAAGGTAAAAGGCCCAAGGGAGCCCTATCGGGCTAAGGGTAAAATTTAAGGATAACCTTCCCAAGGGAAAGGTCTTACCTAAAAAGGTCAAAAGGCCTTTTCAAAAGGCTGGAACTACACTCACTATATTTGTTCGTTTTTAATGATCACTTATTTGTTGTTTTGATGTCCATCACTTGTATTCTGCAACATTAAACAGTTCCCCTTACTAAAACATGAAGCAACTATAATGTTTTCTATTTTTCATAAAATGCTAAGTAAAAAAAGTAATACCGAATCCCTTGGTATTTCTACCCAGCCAACACTGTCGGTG  >  1/1339310‑1339751                                                                                                                                                                                                                                                      |                                                                                                                                                                                                      aaCACTGATAATACTCTTTCTTG‑AGAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTCCAA‑‑‑‑CACGGAT‑‑‑‑CTA‑‑CA‑‑TACCTTGAAATCATACAACAAAACCGCAAAAAGGTAAAAGGAACAAGGGAGCCCTAGCGGGCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATAtttatc                                                                                                                                                                                     <  1:800660/251‑4 (MQ=255)       gATAATACTCTTTCTTGAAAG‑TGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTCCAA‑‑‑‑CACGGAT‑‑‑‑CTA‑‑CA‑‑TACCTTGAAATCATACAACAAAACCGCAAAAAGGTAAAAGGAACAAGGGAGCCCTAGCGGGCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATAtttatctgtttt                                                                                                                                                                               >  1:307693/1‑242 (MQ=255)        ataataCTCTTTCTTGAAAG‑TGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTCCAA‑‑‑‑CACGGAT‑‑‑‑CTA‑‑CA‑‑TACCTTGAAATCATACAACAAAACCGCAAAAAGGTAAAAGGAACAAGGGAGCCCTAGCGGGCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATAtttatctgttttt                                                                                                                                                                              >  1:654575/1‑241 (MQ=255)            taCTCTTTCTTGAAAG‑TGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTCCAA‑‑‑‑CACGGAT‑‑‑‑CTA‑‑CA‑‑TACCTTGAAATCATACAACAAAACCGCAAAAAGGTAAAAGGAACAAGGGAGCCCTAGCGGGCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATg                                                                                                                                                                          <  1:353378/250‑1 (MQ=255)                                                                                    ctccaacacggatctacatacctTGAAATCATACAACAAAACCGCAAAAAGGTAAAAGGAACAAGGGAGCCCTAGCGGGCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATGATCACTTATTTGTTGTTTTGATATTCATCACTTGTATTc                                                                                                                                   >  1:397000/22‑231 (MQ=255)                                                                                    ctccaacacggatctacatacctTGAAATCATACAACAAAACCGCAAAAAGGTAAAAGGAACAAGGGAGCCCTAGCGGGCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATGATCACTTATTTGTTGTTTTGATATTCATCACTTGTATTCTGCAACATTaaat                                                                                                                      >  1:690409/22‑243 (MQ=255)                                                                                              gatctacatacctTGAAATCATACAACAAAACCGCAAAAAGGTAAAAGGAACAAGGGAGCCCTAGCGGGCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAAt                                                                                                                                                                           >  1:660186/12‑181 (MQ=255)                                                                                              gatctacatacctTGAAATCATACAACAAAACCGCAAAAAGGTAAAAGGAACAAGGGAGCCCTAGCGGGCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATg                                                                                                                                                                          <  1:792599/171‑1 (MQ=255)                                                                                              gatctacatacctTGAAATCATACAACAAAACCGCAAAAAGGTAAAAGGAACAAGGGAGCCCTAGCGGGCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATGATCACTTATTTGTTGTTTTGATATTCATCACTTGTATTCTGCAACATTaaat                                                                                                                      <  1:604511/223‑2 (MQ=255)                                                                                              gatctacatacctTGAAATCATACAACAAAACCGCAAAAAGGTAAAAGGAACAAGGGAGCCCTAGCGGGCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATGATCACTTATTTGTTGTTTTGATATTCATCACTTGTATTCTGCAACATTAAATAACACCCCTTACTaag                                                                                                      <  1:626096/239‑2 (MQ=255)                                                                                                  tacatacctTGAAATCATACAACAAAACCGCAAAAAGGTAAAAGGAACAAGGGAGCCCTAGCGGGCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATGATCACTTATTTGTTGTTTTGATATTCATCACTTGTATTCTGCAACATTAAATAACACCCCTTACTaagatatg                                                                                                 >  1:541017/8‑245 (MQ=255)                                                                                                   acatacctTGAAATCATACAACAAAACCGCAAAAAGGTAAAAGGAACAAGGGAGCCCTAGCGGGCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATGATCACTTATTTGTTGTTTTGATATTCATCACTTGTATTCTGCAACATTaaataac                                                                                                                   <  1:197498/226‑5 (MQ=255)                                                                                                     atacctTGAAATCATACAACAAAACCGCAAAAAGGTAAAAGGAACAAGGGAGCCCTAGCGGGCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATGATCACTTATTTGTTGTTTTGATATTCATCACTTGTATTCTGCAACATTAAATAACACCCCTTACTAAGATATGAAg                                                                                              <  1:190343/247‑1 (MQ=255)                                                                                                         ctTGAAATCATACAACAAAACCGCAAAAAGGTAAAAGGAACAAGGGAGCCCTAGCGGGCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATGATCACTTATTTGTTGTTTTGATATTCATCACTTGTATTCTGCAACATTAAATAACACCCCTTACTAAGATATGAAGCAAc                                                                                          <  1:360243/251‑1 (MQ=255)                                                                                                              aaTCATACAACAAAACCGCAAAAAGGTAAAAGGAACAAGGGAGCCCTAGCGGGCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATGATCACTTATTTGTTGTTTTGATATTCATCACTTGTATTCTGCAACATTAAATAACACCCCTTACTAAGATATGAAGCAACtatgt                                                                                     <  1:210347/251‑3 (MQ=255)                                                                                                                tCATACAACAAAACCGCAAAAAGGTAAAAGGAACAAGGGAGCCCTAGCGGGCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAAt                                                                                                                                                                           >  1:582822/1‑163 (MQ=255)                                                                                                                     caacaaAACCGCAAAAAGGTAAAAGGAACAAGGGAGCCCTAGCGGGCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATGATCACTTATTTGTTGTTTTGATATTCATCACTTGTATTCTGCAACATTAAATAACACCCCTTACTAAGATATGAAGCAACtatgttttccat                                                                              <  1:234863/251‑10 (MQ=255)                                                                                                                                            aggaacAAGGGAGCCCTAGCGGGCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATGATCACTTATTTGTTGTTTTGATATTCATCACTTGTATTCTGCAACATTAAATAACACCCCTTACTAAGATATGAAGCAACT‑‑‑ATGTTTTCCATTTTTCATAAAATCCTAAGTaaaa                                                    >  1:416088/6‑251 (MQ=255)                                                                                                                                                      gAGCCCTAGCGGGCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATAtttatc                                                                                                                                                                                     >  1:786621/1‑112 (MQ=255)                                                                                                                                                        gCCCTAGCGGGCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATGATCACTTATTTGTTGTTTTGATATTCATCACTTGTATTCTGCAACATTaaataacaccccttacta                                                                                                        <  1:63624/190‑16 (MQ=255)                                                                                                                                                           ctagcGGGCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATGATCACTTATTTGTTGTTTTGATATTCATCACTTGTATTCTGCAACATTAAATAACACCCCTTACTaag                                                                                                      <  1:572970/185‑2 (MQ=255)                                                                                                                                                             agcGGGCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATGATCACTTATTTGTTGTTTTGATATTCATCACTTGTATTCTGCAACATTAAATAACACCCCTTACTAAGATATGAAGCAACT‑‑‑ATGTTTTCCATTTTTCATAAAATCCTAAGTAAAAAATCTAATAc                                          <  1:98711/242‑1 (MQ=255)                                                                                                                                                                  gCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATGATCACTTATTTGTTGTTTTGATATTCATCACTTGTATTCTGCAACATTAAATAACCCCCCTTACTAAGATATGAAGCAACT‑‑‑ATGTTTTCCATTTTTCATAAAATCCTAAGTAAAAAATCTAATACCGAATCCTTTga                              >  1:442552/1‑250 (MQ=255)                                                                                                                                                                       gggTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATGATCACTTATTTGTTGTTTTGATATTCATCACTTGTATTCTGCAACATTAAATAACACCCCTTACTAAGATATGAAGCAACT‑‑‑ATGTTTTCCATTTTTCATAAAATCCTAAGTAAAAAATCTAATACCGAATCCTTTGATAtta                         >  1:131650/1‑250 (MQ=255)                                                                                                                                                                             aaTTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATGATCACTTATTTGTTGTTTTGATATTCATCACTTGTATTCTGc                                                                                                                                <  1:737542/145‑1 (MQ=255)                                                                                                                                                                             aaTTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATGATCACTTATTTGTTGTTTTGATATTCATCACTTGTATTCTGCAACATTAAATAACACCCCTTACTAAGATATGAAGCAACT‑‑‑ATGTTTTCCATTTTTCATAAAATCCTAAGTAAAAAATCTAATACCGAATCCTTTGATATTACTatcc                   <  1:839919/251‑4 (MQ=255)                                                                                                                                                                                        aaCCTTTCCAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATGATCACTTATTTGTTGTTTTGATATTCATCAc                                                                                                                                           >  1:65838/1‑123 (MQ=255)                                                                                                                                                                                              tccAAGGGAAAGGTCTTACCTGAAAAGGCCAAAAGGCTTTTTCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATGATCACTTATTTGTTGTTTTGATATTCATCACTTGTATTCTGCAACATTAAATAACACCCCTTACTAAGATATGAAGCAACT‑‑‑ATGTTTTCCATTTTTCATAAAATCCTAAGTAAAAAATCTAATACCGAATCCTTTGATATTACTATCCAACCGACACtaccaatg  <  1:787157/250‑8 (MQ=255)                                                                                                                                                                                                                                     tttCAAAAGGCAGGAACAACCCGCACCATATTTATCTGTTTTTAATGATCACTTATTTGTTGTTTTGATATTCATCACTTGTATTc                                                                                                                                   >  1:774784/1‑86 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                      AACATTGATAAAACTCTTTCTTGAAGA‑TGAGTTTGCCCATTTTTTGATTTAGCTTGATTTAACATATTTTCTCCAACGCGCGCGGATTGTAATAATGATCTATCTTGGAATCATACAACAAAATTGCCAAAAGGTAAAAGGCCCAAGGGAGCCCTATCGGGCTAAGGGTAAAATTTAAGGATAACCTTCCCAAGGGAAAGGTCTTACCTAAAAAGGTCAAAAGGCCTTTTCAAAAGGCTGGAACTACACTCACTATATTTGTTCGTTTTTAATGATCACTTATTTGTTGTTTTGATGTCCATCACTTGTATTCTGCAACATTAAACAGTTCCCCTTACTAAAACATGAAGCAACTATAATGTTTTCTATTTTTCATAAAATGCTAAGTAAAAAAAGTAATACCGAATCCCTTGGTATTTCTACCCAGCCAACACTGTCGGTG  >  1/1339310‑1339751

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑