Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,339,285	C→T	Q10Q (CAG→CAA)	unknown ←	Haemophilus‑specific protein, uncharacterized

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,339,285	0	C	T	100.0%	139.9 / NA	52	Q10Q (CAG→CAA)	unknown	Haemophilus‑specific protein, uncharacterized
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (24/28);  total (24/28)

AACTAAGTTTTTCTGATAGACACTCTATTTCCTCAATGAATTTGTCAGCTACTTCCTTATTTTGGCTTTGTGTGTAAATATATTCTGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAATGTAGTCTTGTTTCATCGCTTCAATGCCTTGCGCAAATCTTTCATTACATCTTCATGTGAGTAAGTCTTAATTTTTCCATTTAAGACACCTTGTAAAAAGCGCTGCTCTTCTGTTAAGGATTGCTCAAACATTGATAAAACTCTTTCTTGAAGATGAGTTTGCCCATTTTTTGATTTAGCTTGATTTAACATATTTTCTCCAACGCGCGCGGATTGTAATAATGATCTATCTTGGAATCATACAACAAAATTGCCAAAAGGTAAAAGGCCCAAGGGAGCCCTATCGGGCTAAGGGTAAAATTTAAGGATAACCTTCCCAAGGGAAAGGTCTTACCTAAAA  >  1/1339051‑1339522                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                              aaCTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAgg                                                                                                                                                                                                                               <  1:839028/251‑1 (MQ=255)              tGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACac                                                                                                                                                                                                                  <  1:282192/251‑2 (MQ=255)              tGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACac                                                                                                                                                                                                                  <  1:267620/251‑2 (MQ=255)                   gacgctctATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACac                                                                                                                                                                                                                  <  1:24566/242‑2 (MQ=255)                       ctctATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATaatac                                                                                                                                                                                                         <  1:380722/251‑4 (MQ=255)                       ctctATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATaatac                                                                                                                                                                                                         <  1:686141/251‑4 (MQ=255)                       ctctATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATaata                                                                                                                                                                                                          >  1:87181/1‑248 (MQ=255)                         ctATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAgg                                                                                                                                                                                                                               >  1:560529/1‑227 (MQ=255)                         ctATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGac                                                                                                                                                                                                                             <  1:166077/229‑2 (MQ=255)                         ctATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGac                                                                                                                                                                                                                             <  1:142115/229‑2 (MQ=255)                         ctATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATActc                                                                                                                                                                                                       <  1:776313/251‑1 (MQ=255)                         ctATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATActc                                                                                                                                                                                                       <  1:690848/251‑1 (MQ=255)                         ctATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATActc                                                                                                                                                                                                       <  1:848303/251‑1 (MQ=255)                         ctATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATAct                                                                                                                                                                                                        >  1:345053/1‑250 (MQ=255)                                 tCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATaatac                                                                                                                                                                                                         >  1:574803/1‑238 (MQ=255)                                      gAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCtcttcag                                                                                                                                                                                                                                     >  1:417789/1‑206 (MQ=255)                                      gAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGac                                                                                                                                                                                                                             <  1:615472/216‑2 (MQ=255)                                      gAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTg                                                                                                                                                                                                <  1:547591/245‑1 (MQ=255)                                          ttGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGtt                                                                                                                                                                                      >  1:847551/1‑251 (MQ=255)                                                      tCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCAtttttt                                                                                                                                                                          >  1:664728/1‑251 (MQ=255)                                                          tactttGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATaatac                                                                                                                                                                                                         >  1:565035/4‑213 (MQ=255)                                                                      gtgtgtAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTa                                                                                                                                                                                                                                  <  1:7649/179‑1 (MQ=255)                                                                           tAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACat                                                                                                                                                      <  1:482085/250‑1 (MQ=255)                                                                             aaTATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACat                                                                                                                                                      >  1:667739/1‑248 (MQ=255)                                                                             aaTATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATAtt                                                                                                                                                   >  1:451611/1‑251 (MQ=255)                                                                             aaTATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATAtt                                                                                                                                                   >  1:312984/1‑251 (MQ=255)                                                                                   ttccgCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTcc                                                                                                                                             <  1:533506/247‑1 (MQ=255)                                                                                   ttccgCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTcc                                                                                                                                             <  1:481236/247‑1 (MQ=255)                                                                                        cTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTCCAAcac                                                                                                                                        <  1:852226/251‑3 (MQ=255)                                                                                            aTCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTCCAAcacggat                                                                                                                                    >  1:304981/1‑245 (MQ=255)                                                                                             tCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTCCAAcacggatc                                                                                                                                   <  1:633588/251‑8 (MQ=255)                                                                                                  aaGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTCCAAcacggatctacat                                                                                                                              <  1:479013/251‑13 (MQ=255)                                                                                                    gCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTCCAAcacggatctacata                                                                                                                             >  1:128175/1‑237 (MQ=255)                                                                                                     cTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTCCAAcacggatctacatac                                                                                                                            <  1:741916/250‑15 (MQ=255)                                                                                                     cTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTCCAAcacggatctacatac                                                                                                                            <  1:745072/250‑15 (MQ=255)                                                                                                          aTCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGCACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTCCAAcacggatctacataccttgaa                                                                                                                      >  1:451424/1‑231 (MQ=255)                                                                                                                          ccATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAgg                                                                                                                                                                                                                               >  1:806915/1‑130 (MQ=255)                                                                                                                          ccATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGac                                                                                                                                                                                                                             <  1:695953/132‑2 (MQ=255)                                                                                                                                    acgtaatcctGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGac                                                                                                                                                                                                                             >  1:240240/10‑121 (MQ=255)                                                                                                                                    acgtaatcctGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACac                                                                                                                                                                                                                  <  1:24671/124‑2 (MQ=255)                                                                                                                                      gtaatcctGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTCCAAcacggatctacatac                                                                                                                            >  1:227763/8‑203 (MQ=255)                                                                                                                                        aatcctGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTCCAAcacggatctacatac                                                                                                                            >  1:277116/6‑201 (MQ=255)                                                                                                                                                       tttCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCAttttttaat                                                                                                                                                                       <  1:701368/156‑4 (MQ=255)                                                                                                                                                                               cATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTCCAAcacggatctacatac                                                                                                                            >  1:728756/1‑162 (MQ=255)                                                                                                                                                                                   acctCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTCCAA‑‑‑‑CACGGAT‑‑‑‑CTA‑‑CA‑‑TACCTTGAAATCATACAACAAAACCGCAAAAAGGTAAAAGGAACAAGGGAGCCCTAGCGGGCTAAGGGTAAAATTTAAGGAt                                 >  1:109841/4‑251 (MQ=255)                                                                                                                                                                                     ctCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTCCAA‑‑‑‑CACGGAT‑‑‑‑CTA‑‑CA‑‑TACCTTGAAATCATACAACAAAACCGCAAAAAGGTAAAAGGAACAAGGGAGCCCTAGCGGGCTAAGGGTAAAATTTAAGGATaa                               >  1:692483/2‑251 (MQ=255)                                                                                                                                                                                            tgtgAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTCCAA‑‑‑‑CACGGAT‑‑‑‑CTA‑‑CA‑‑TACCTTGAAATCATACAACAAAACCGCAAAAAGGTAAAAGGAACAAGGGAGCCCTAGCGGGCTAAGGGTAAAATTTAAGGATAACCtttc                         >  1:768890/1‑248 (MQ=255)                                                                                                                                                                                            tgtgAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTCCAA‑‑‑‑CACGGAT‑‑‑‑CTA‑‑CA‑‑TACCTTGAAATCATACAACAAAACCGCAAAAAGGTAAAAGGAACAAGGGAGCCCTAGCGGGCTAAGGGTAAAATTTAAGGATAACCtttc                         >  1:708263/1‑248 (MQ=255)                                                                                                                                                                                            cgtagctttgtcgtAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTCCAA‑‑‑‑CACGGAT‑‑‑‑CTA‑‑CA‑‑TACCTTGAAATCATACAACAAAACCGCAAAAAGGTAAAAGGAACAAGGGAGCCCTAGCGGGCTAAGGGTAAAATTTAAGGATAAcc                             <  1:164276/233‑1 (MQ=255)                                                                                                                                                                                                     gTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTCCAAcacggatctacatac                                                                                                                            >  1:873354/1‑140 (MQ=255)                                                                                                                                                                                                                  cATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTCCAA‑‑‑‑CACGGAT‑‑‑‑CTA‑‑CA‑‑TACCTTGAAATCATACAACAAAACCGCAAAAAGGTAAAAGGAACAAGGGAGCCCTAGCGGGCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTgaaa  <  1:137420/251‑1 (MQ=255)                                                                                                                                                                                                                      caaGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTCCAA‑‑‑‑CACGGAT‑‑‑‑CTA‑‑CA‑‑TACCTTGAAATCATACAACAAAACCGCAAAAAGGTAAAAGGAACAAGGGAGCCCTAGCGGGCTAAGGGTAAAATTTAAGGATAACCTTTCCAAGGGAAAGGTCTTACCTgaaa  <  1:702711/246‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                              AACTAAGTTTTTCTGATAGACACTCTATTTCCTCAATGAATTTGTCAGCTACTTCCTTATTTTGGCTTTGTGTGTAAATATATTCTGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAATGTAGTCTTGTTTCATCGCTTCAATGCCTTGCGCAAATCTTTCATTACATCTTCATGTGAGTAAGTCTTAATTTTTCCATTTAAGACACCTTGTAAAAAGCGCTGCTCTTCTGTTAAGGATTGCTCAAACATTGATAAAACTCTTTCTTGAAGATGAGTTTGCCCATTTTTTGATTTAGCTTGATTTAACATATTTTCTCCAACGCGCGCGGATTGTAATAATGATCTATCTTGGAATCATACAACAAAATTGCCAAAAGGTAAAAGGCCCAAGGGAGCCCTATCGGGCTAAGGGTAAAATTTAAGGATAACCTTCCCAAGGGAAAGGTCTTACCTAAAA  >  1/1339051‑1339522

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑