Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,339,201	C→T	K38K (AAG→AAA)	unknown ←	Haemophilus‑specific protein, uncharacterized

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,339,201	0	C	T	100.0%	201.9 / NA	76	K38K (AAG→AAA)	unknown	Haemophilus‑specific protein, uncharacterized
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (36/40);  total (36/40)

TCATAATATAGGTTCCAACAACAAGAAACTTTACTGAATGCCCATTTTTAAGAGGGAAAATATGAAACTTCCCGTCGGTATAGGCATCAGCAATATAACTAAGTTTTTCTGATAGACACTCTATTTCCTCAATGAATTTGTCAGCTACTTCCTTATTTTGGCTTTGTGTGTAAATATATTCTGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAATGTAGTCTTGTTTCATCGCTTCAATGCCTTGCGCAAATCTTTCATTACATCTTCATGTGAGTAAGTCTTAATTTTTCCATTTAAGACACCTTGTAAAAAGCGCTGCTCTTCTGTTAAGGATTGCTCAAACATTGATAAAACTCTTTCTTGAAGATGAGTTTGCCCATTTTTTGATTTAGCTTGATTTAACATATTTTCTCCAACGCGCGCGGATTGTAATAATG  >  1/1338955‑1339406                                                                                                                                                                                                                                                       |                                                                                                                                                                                                              tCATAATATACATTCCTACAACCAGAAACTTCACTGAATGCCCATTTTTAAGTGGAAAAATGTGAAATCTCCCGTCAGTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCa                                                                                                                                                                                                           >  1:400564/1‑251 (MQ=255) tCATAATATACATTCCTACAACCAGAAACTTCACTGAATGCCCATTTTTAAGTGGAAAAATGTGAAATCTCCCGTCAGTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCa                                                                                                                                                                                                           >  1:744739/1‑251 (MQ=255)   ataataTACATTCCTACAACCAGAAACTTCACTGAATGCCCATTTTTAAGTGGAAAAATGTGAAATCTCCCGTCAGTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAAt                                                                                                                                                                                                         >  1:491142/1‑251 (MQ=255)      atatacattCCTACAACCAGAAACTTCACTGAATGCCCATTTTTAAGTGGAAAAATGTGAAATCTCCCGTCAGTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGc                                                                                                                                                                                                       >  1:623153/8‑250 (MQ=255)       tatacattCCTACAACCAGAAACTTCACTGAATGCCCATTTTTAAGTGGAAAAATGTGAAATCTCCCGTCAGTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCt                                                                                                                                                                                                     <  1:665540/245‑1 (MQ=255)                             cttcaCTGAATGCCCATTTTTAAGTGGAAAAATGTGAAATCTCCCGTCAGTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTAcct                                                                                                                                                                                >  1:857298/5‑248 (MQ=255)                                   tGAATGCCCATTTTTAAGTGGAAAAATGTGAAATCTCCCGTCAGTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCAtg                                                                                                                                                                         <  1:410129/251‑1 (MQ=255)                                   tGAATGCCCATTTTTAAGTGGAAAAATGTGAAATCTCCCGTCAGTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCAtg                                                                                                                                                                         <  1:421895/251‑1 (MQ=255)                                     aaTGCCCATTTTTAAGTGGAAAAATGTGAAATCTCCCGTCAGTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCAtgtg                                                                                                                                                                       <  1:228148/251‑1 (MQ=255)                                     aaTGCCCATTTTTAAGTGGAAAAATGTGAAATCTCCCGTCAGTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCAtgtg                                                                                                                                                                       <  1:277073/251‑1 (MQ=255)                                           cATTTTTAAGTGGAAAAATGTGAAATCTCCCGTCAGTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCAt                                                                                                                                                                                     >  1:552000/1‑231 (MQ=255)                                            aTTTTTAAGTGGAAAAATGTGAAATCTCCCGTCAGTATAGGCGTCAGCAATCTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTaggt                                                                                                                                                                >  1:450901/1‑248 (MQ=255)                                             tttttAAGTGGAAAAATGTGAAATCTCCCGTCAGTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTc                                                                                                                                                               <  1:188738/251‑1 (MQ=255)                                             tttttAAGTGGAAAAATGTGAAATCTCCCGTCAGTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTc                                                                                                                                                               <  1:669865/251‑1 (MQ=255)                                                  aagtggaaaaaTGTGAAATCTCCCGTCAGTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAAt                                                                                                                                                          <  1:10290/244‑1 (MQ=255)                                                  aagtggaaaaaTGTGAAATCTCCCGTCAGTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAAt                                                                                                                                                          <  1:840595/244‑1 (MQ=255)                                                              gtgaaatctCCCGTCAGTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCAttcaa                                                                                                                                              >  1:50896/9‑248 (MQ=255)                                                                    tctCCCGTCAGTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCAtgtg                                                                                                                                                                       <  1:467730/218‑1 (MQ=255)                                                                       cccGTCAGTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTg                                                                                                                                     >  1:826244/1‑251 (MQ=255)                                                                         cGTCAGTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTa                                                                                                                                   >  1:508055/1‑251 (MQ=255)                                                                            cagTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGc                                                                                                                                                                                                       >  1:144242/3‑180 (MQ=255)                                                                            cagTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGac                                                                                                                                           <  1:109825/238‑1 (MQ=255)                                                                             agTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTaaaaa                                                                                                                               >  1:2064/2‑251 (MQ=255)                                                                              gTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGTGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAg                                                                                                                              >  1:504438/1‑251 (MQ=255)                                                                              gTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAg                                                                                                                              >  1:790983/1‑251 (MQ=255)                                                                              gTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAg                                                                                                                              >  1:883757/1‑251 (MQ=255)                                                                               tataGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTaaaa                                                                                                                                <  1:265917/248‑1 (MQ=255)                                                                                   ggcgtCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCAtg                                                                                                                                                                         <  1:821727/199‑1 (MQ=255)                                                                                             atgtaACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCaa                                                                                                                                                                                                          <  1:859494/157‑1 (MQ=255)                                                                                                 aaCTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAgg                                                                                                           <  1:839028/251‑1 (MQ=255)                                                                                                              tGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACac                                                                                              <  1:282192/251‑2 (MQ=255)                                                                                                              tGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACac                                                                                              <  1:267620/251‑2 (MQ=255)                                                                                                                   gacgctctATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACac                                                                                              <  1:24566/242‑2 (MQ=255)                                                                                                                       ctctATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCAtgtg                                                                                                                                                                       <  1:430895/169‑1 (MQ=255)                                                                                                                       ctctATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACAcc                                                                                                                                        >  1:814858/1‑200 (MQ=255)                                                                                                                       ctctATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATaatac                                                                                     <  1:380722/251‑4 (MQ=255)                                                                                                                       ctctATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATaatac                                                                                     <  1:686141/251‑4 (MQ=255)                                                                                                                       ctctATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATaata                                                                                      >  1:87181/1‑248 (MQ=255)                                                                                                                         ctATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAgg                                                                                                           >  1:560529/1‑227 (MQ=255)                                                                                                                         ctATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGac                                                                                                         <  1:166077/229‑2 (MQ=255)                                                                                                                         ctATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGac                                                                                                         <  1:142115/229‑2 (MQ=255)                                                                                                                         ctATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATActc                                                                                   <  1:848303/251‑1 (MQ=255)                                                                                                                         ctATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATActc                                                                                   <  1:776313/251‑1 (MQ=255)                                                                                                                         ctATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATActc                                                                                   <  1:690848/251‑1 (MQ=255)                                                                                                                         ctATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATAct                                                                                    >  1:345053/1‑250 (MQ=255)                                                                                                                                 tCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATaatac                                                                                     >  1:574803/1‑238 (MQ=255)                                                                                                                                    aTGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAg                                                                                                                                                                     >  1:135188/1‑158 (MQ=255)                                                                                                                                      gAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCtcttcag                                                                                                                 >  1:417789/1‑206 (MQ=255)                                                                                                                                      gAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGac                                                                                                         <  1:615472/216‑2 (MQ=255)                                                                                                                                      gAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTg                                                                            <  1:547591/245‑1 (MQ=255)                                                                                                                                          ttGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGtt                                                                  >  1:847551/1‑251 (MQ=255)                                                                                                                                                    cTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAAtt                                                                                                                                                         >  1:546373/1‑154 (MQ=255)                                                                                                                                                      tCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCAtttttt                                                      >  1:664728/1‑251 (MQ=255)                                                                                                                                                          tactttGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATaatac                                                                                     >  1:565035/4‑213 (MQ=255)                                                                                                                                                                      gtgtgtAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTa                                                                                                              <  1:7649/179‑1 (MQ=255)                                                                                                                                                                           tAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACat                                  <  1:482085/250‑1 (MQ=255)                                                                                                                                                                             aaTATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACat                                  >  1:667739/1‑248 (MQ=255)                                                                                                                                                                             aaTATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATAtt                               >  1:451611/1‑251 (MQ=255)                                                                                                                                                                             aaTATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATAtt                               >  1:312984/1‑251 (MQ=255)                                                                                                                                                                                   ttccgCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTcc                         <  1:481236/247‑1 (MQ=255)                                                                                                                                                                                   ttccgCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTcc                         <  1:533506/247‑1 (MQ=255)                                                                                                                                                                                        cTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTCCAAcac                    <  1:852226/251‑3 (MQ=255)                                                                                                                                                                                            aTCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTAcctc                                                                                                                                                                               <  1:382575/92‑4 (MQ=255)                                                                                                                                                                                            aTCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTCCAAcacggat                >  1:304981/1‑245 (MQ=255)                                                                                                                                                                                             tCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTCCAAcacggatc               <  1:633588/251‑8 (MQ=255)                                                                                                                                                                                                  aaGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTCCAAcacggatctacat          <  1:479013/251‑13 (MQ=255)                                                                                                                                                                                                    gCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTCCAAcacggatctacata         >  1:128175/1‑237 (MQ=255)                                                                                                                                                                                                     cTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTCCAAcacggatctacatac        <  1:745072/250‑15 (MQ=255)                                                                                                                                                                                                     cTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTCCAAcacggatctacatac        <  1:741916/250‑15 (MQ=255)                                                                                                                                                                                                          aTCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGCACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTCCAAcacggatctacataccttgaa  >  1:451424/1‑231 (MQ=255)                                                                                                                                                                                                                          ccATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAgg                                                                                                           >  1:806915/1‑130 (MQ=255)                                                                                                                                                                                                                          ccATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGac                                                                                                         <  1:695953/132‑2 (MQ=255)                                                                                                                                                                                                                                    acgtaatcctGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGac                                                                                                         >  1:240240/10‑121 (MQ=255)                                                                                                                                                                                                                                    acgtaatcctGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACac                                                                                              <  1:24671/124‑2 (MQ=255)                                                                                                                                                                                                                                      gtaatcctGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTCCAAcacggatctacatac        >  1:227763/8‑203 (MQ=255)                                                                                                                                                                                                                                        aatcctGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTCCAAcacggatctacatac        >  1:277116/6‑201 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                              TCATAATATAGGTTCCAACAACAAGAAACTTTACTGAATGCCCATTTTTAAGAGGGAAAATATGAAACTTCCCGTCGGTATAGGCATCAGCAATATAACTAAGTTTTTCTGATAGACACTCTATTTCCTCAATGAATTTGTCAGCTACTTCCTTATTTTGGCTTTGTGTGTAAATATATTCTGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAATGTAGTCTTGTTTCATCGCTTCAATGCCTTGCGCAAATCTTTCATTACATCTTCATGTGAGTAAGTCTTAATTTTTCCATTTAAGACACCTTGTAAAAAGCGCTGCTCTTCTGTTAAGGATTGCTCAAACATTGATAAAACTCTTTCTTGAAGATGAGTTTGCCCATTTTTTGATTTAGCTTGATTTAACATATTTTCTCCAACGCGCGCGGATTGTAATAATG  >  1/1338955‑1339406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑