Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,339,187	G→A	D4D (GAC→GAT)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,339,187	0	G	A	100.0%	197.3 / NA	74	D4D (GAC→GAT)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (34/40);  total (34/40)

CGTAAATCATAATATAGGTTCCAACAACAAGAAACTTTACTGAATGCCCATTTTTAAGAGGGAAAATATGAAACTTCCCGTCGGTATAGGCATCAGCAATATAACTAAGTTTTTCTGATAGACACTCTATTTCCTCAATGAATTTGTCAGCTACTTCCTTATTTTGGCTTTGTGTGTAAATATATTCTGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAATGTAGTCTTGTTTCATCGCTTCAATGCCTTGCGCAAATCTTTCATTACATCTTCATGTGAGTAAGTCTTAATTTTTCCATTTAAGACACCTTGTAAAAAGCGCTGCTCTTCTGTTAAGGATTGCTCAAACATTGATAAAACTCTTTCTTGAAGATGAGTTTGCCCATTTTTTGATTTAGCTTGATTTAACATATTTTCTCCAACGCGCGCGGATTGTAATAATG  >  1/1338949‑1339406                                                                                                                                                                                                                                               |                                                                                                                                                                                                                            cGTAAATCATAATATACATTCCTACAACCAGAAACTTCACTGAATGCCCATTTTTAAGTGGAAAAATGTGAAATCTCCCGTCAGTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCAt                                                                                                                                                                                                                  <  1:657619/250‑1 (MQ=255)       tCATAATATACATTCCTACAACCAGAAACTTCACTGAATGCCCATTTTTAAGTGGAAAAATGTGAAATCTCCCGTCAGTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCa                                                                                                                                                                                                           >  1:400564/1‑251 (MQ=255)       tCATAATATACATTCCTACAACCAGAAACTTCACTGAATGCCCATTTTTAAGTGGAAAAATGTGAAATCTCCCGTCAGTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCa                                                                                                                                                                                                           >  1:744739/1‑251 (MQ=255)         ataataTACATTCCTACAACCAGAAACTTCACTGAATGCCCATTTTTAAGTGGAAAAATGTGAAATCTCCCGTCAGTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAAt                                                                                                                                                                                                         >  1:491142/1‑251 (MQ=255)            atatacattCCTACAACCAGAAACTTCACTGAATGCCCATTTTTAAGTGGAAAAATGTGAAATCTCCCGTCAGTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGc                                                                                                                                                                                                       >  1:623153/8‑250 (MQ=255)             tatacattCCTACAACCAGAAACTTCACTGAATGCCCATTTTTAAGTGGAAAAATGTGAAATCTCCCGTCAGTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCt                                                                                                                                                                                                     <  1:665540/245‑1 (MQ=255)                                   cttcaCTGAATGCCCATTTTTAAGTGGAAAAATGTGAAATCTCCCGTCAGTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTAcct                                                                                                                                                                                >  1:857298/5‑248 (MQ=255)                                         tGAATGCCCATTTTTAAGTGGAAAAATGTGAAATCTCCCGTCAGTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCAtg                                                                                                                                                                         <  1:421895/251‑1 (MQ=255)                                         tGAATGCCCATTTTTAAGTGGAAAAATGTGAAATCTCCCGTCAGTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCAtg                                                                                                                                                                         <  1:410129/251‑1 (MQ=255)                                           aaTGCCCATTTTTAAGTGGAAAAATGTGAAATCTCCCGTCAGTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCAtgtg                                                                                                                                                                       <  1:228148/251‑1 (MQ=255)                                           aaTGCCCATTTTTAAGTGGAAAAATGTGAAATCTCCCGTCAGTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCAtgtg                                                                                                                                                                       <  1:277073/251‑1 (MQ=255)                                                 cATTTTTAAGTGGAAAAATGTGAAATCTCCCGTCAGTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCAt                                                                                                                                                                                     >  1:552000/1‑231 (MQ=255)                                                  aTTTTTAAGTGGAAAAATGTGAAATCTCCCGTCAGTATAGGCGTCAGCAATCTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTaggt                                                                                                                                                                >  1:450901/1‑248 (MQ=255)                                                   tttttAAGTGGAAAAATGTGAAATCTCCCGTCAGTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTc                                                                                                                                                               <  1:669865/251‑1 (MQ=255)                                                   tttttAAGTGGAAAAATGTGAAATCTCCCGTCAGTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTc                                                                                                                                                               <  1:188738/251‑1 (MQ=255)                                                        aagtggaaaaaTGTGAAATCTCCCGTCAGTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAAt                                                                                                                                                          <  1:840595/244‑1 (MQ=255)                                                        aagtggaaaaaTGTGAAATCTCCCGTCAGTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAAt                                                                                                                                                          <  1:10290/244‑1 (MQ=255)                                                                    gtgaaatctCCCGTCAGTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCAttcaa                                                                                                                                              >  1:50896/9‑248 (MQ=255)                                                                          tctCCCGTCAGTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCAtgtg                                                                                                                                                                       <  1:467730/218‑1 (MQ=255)                                                                             cccGTCAGTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTg                                                                                                                                     >  1:826244/1‑251 (MQ=255)                                                                               cGTCAGTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTa                                                                                                                                   >  1:508055/1‑251 (MQ=255)                                                                                  cagTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGc                                                                                                                                                                                                       >  1:144242/3‑180 (MQ=255)                                                                                  cagTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGac                                                                                                                                           <  1:109825/238‑1 (MQ=255)                                                                                   agTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTaaaaa                                                                                                                               >  1:2064/2‑251 (MQ=255)                                                                                    gTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGTGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAg                                                                                                                              >  1:504438/1‑251 (MQ=255)                                                                                    gTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAg                                                                                                                              >  1:790983/1‑251 (MQ=255)                                                                                    gTATAGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAg                                                                                                                              >  1:883757/1‑251 (MQ=255)                                                                                     tataGGCGTCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTaaaa                                                                                                                                <  1:265917/248‑1 (MQ=255)                                                                                         ggcgtCAGCAATGTAACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCAtg                                                                                                                                                                         <  1:821727/199‑1 (MQ=255)                                                                                                   atgtaACTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCaa                                                                                                                                                                                                          <  1:859494/157‑1 (MQ=255)                                                                                                       aaCTAAGTTTTTCTGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAgg                                                                                                           <  1:839028/251‑1 (MQ=255)                                                                                                                    tGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACac                                                                                              <  1:267620/251‑2 (MQ=255)                                                                                                                    tGATAGACGCTCTATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACac                                                                                              <  1:282192/251‑2 (MQ=255)                                                                                                                         gacgctctATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACac                                                                                              <  1:24566/242‑2 (MQ=255)                                                                                                                             ctctATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCAtgtg                                                                                                                                                                       <  1:430895/169‑1 (MQ=255)                                                                                                                             ctctATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACAcc                                                                                                                                        >  1:814858/1‑200 (MQ=255)                                                                                                                             ctctATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATaatac                                                                                     <  1:380722/251‑4 (MQ=255)                                                                                                                             ctctATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATaatac                                                                                     <  1:686141/251‑4 (MQ=255)                                                                                                                             ctctATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATaata                                                                                      >  1:87181/1‑248 (MQ=255)                                                                                                                               ctATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAgg                                                                                                           >  1:560529/1‑227 (MQ=255)                                                                                                                               ctATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGac                                                                                                         <  1:142115/229‑2 (MQ=255)                                                                                                                               ctATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGac                                                                                                         <  1:166077/229‑2 (MQ=255)                                                                                                                               ctATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATActc                                                                                   <  1:690848/251‑1 (MQ=255)                                                                                                                               ctATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATActc                                                                                   <  1:776313/251‑1 (MQ=255)                                                                                                                               ctATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATActc                                                                                   <  1:848303/251‑1 (MQ=255)                                                                                                                               ctATTTCCTCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATAct                                                                                    >  1:345053/1‑250 (MQ=255)                                                                                                                                       tCAATGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATaatac                                                                                     >  1:574803/1‑238 (MQ=255)                                                                                                                                          aTGAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAg                                                                                                                                                                     >  1:135188/1‑158 (MQ=255)                                                                                                                                            gAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCtcttcag                                                                                                                 >  1:417789/1‑206 (MQ=255)                                                                                                                                            gAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGac                                                                                                         <  1:615472/216‑2 (MQ=255)                                                                                                                                            gAATTTGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTg                                                                            <  1:547591/245‑1 (MQ=255)                                                                                                                                                ttGTCAGCCACTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGtt                                                                  >  1:847551/1‑251 (MQ=255)                                                                                                                                                          cTTCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAAtt                                                                                                                                                         >  1:546373/1‑154 (MQ=255)                                                                                                                                                            tCCTTACTTTGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCAtttttt                                                      >  1:664728/1‑251 (MQ=255)                                                                                                                                                                tactttGGCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATaatac                                                                                     >  1:565035/4‑213 (MQ=255)                                                                                                                                                                      ggCTTTGTGTGTAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCAt                                                                                                                                                                                                                  >  1:439411/1‑85 (MQ=255)                                                                                                                                                                            gtgtgtAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTa                                                                                                              <  1:7649/179‑1 (MQ=255)                                                                                                                                                                                 tAAATATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACat                                  <  1:482085/250‑1 (MQ=255)                                                                                                                                                                                   aaTATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACat                                  >  1:667739/1‑248 (MQ=255)                                                                                                                                                                                   aaTATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATAtt                               >  1:451611/1‑251 (MQ=255)                                                                                                                                                                                   aaTATATTCCGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATAtt                               >  1:312984/1‑251 (MQ=255)                                                                                                                                                                                         ttccgCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTcc                         <  1:533506/247‑1 (MQ=255)                                                                                                                                                                                         ttccgCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTcc                         <  1:481236/247‑1 (MQ=255)                                                                                                                                                                                              cTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTCCAAcac                    <  1:852226/251‑3 (MQ=255)                                                                                                                                                                                                  aTCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTAcctc                                                                                                                                                                               <  1:382575/92‑4 (MQ=255)                                                                                                                                                                                                  aTCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTCCAAcacggat                >  1:304981/1‑245 (MQ=255)                                                                                                                                                                                                   tCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTCCAAcacggatc               <  1:633588/251‑8 (MQ=255)                                                                                                                                                                                                        aaGCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTCCAAcacggatctacat          <  1:479013/251‑13 (MQ=255)                                                                                                                                                                                                          gCTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTCCAAcacggatctacata         >  1:128175/1‑237 (MQ=255)                                                                                                                                                                                                           cTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTCCAAcacggatctacatac        <  1:745072/250‑15 (MQ=255)                                                                                                                                                                                                           cTGAAATCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTCCAAcacggatctacatac        <  1:741916/250‑15 (MQ=255)                                                                                                                                                                                                                aTCTAGCCATTTCAGACCATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGCACTGTTCAAACACTGATAATACTCTTTCTTGAAAGTGAGTTTGCTCATTTTTTAATTTAGCTTGATTTAACATATTTTCTCCAAcacggatctacataccttgaa  >  1:451424/1‑231 (MQ=255)                                                                                                                                                                                                                                ccATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAgg                                                                                                           >  1:806915/1‑130 (MQ=255)                                                                                                                                                                                                                                ccATTGAACAACGTAATCCTGTTTCATCGTTTCAATGCCTTGCGCAAATCTTTCATTACCTCTTCATGTGAGTAGGTCTTAATTTTGCCATTCAAGACACCTTGTAAAAAGCGTTGCTCTTCAGTTAAGGac                                                                                                         <  1:695953/132‑2 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                            CGTAAATCATAATATAGGTTCCAACAACAAGAAACTTTACTGAATGCCCATTTTTAAGAGGGAAAATATGAAACTTCCCGTCGGTATAGGCATCAGCAATATAACTAAGTTTTTCTGATAGACACTCTATTTCCTCAATGAATTTGTCAGCTACTTCCTTATTTTGGCTTTGTGTGTAAATATATTCTGCTTTATCTAAAAGCTGAAATCTAGCCATTTCAGACCATTGAACAATGTAGTCTTGTTTCATCGCTTCAATGCCTTGCGCAAATCTTTCATTACATCTTCATGTGAGTAAGTCTTAATTTTTCCATTTAAGACACCTTGTAAAAAGCGCTGCTCTTCTGTTAAGGATTGCTCAAACATTGATAAAACTCTTTCTTGAAGATGAGTTTGCCCATTTTTTGATTTAGCTTGATTTAACATATTTTCTCCAACGCGCGCGGATTGTAATAATG  >  1/1338949‑1339406

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑