Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,329,038	C→T	T110T (ACG→ACA)	unknown ←	Programmed cell death toxin MazF

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,329,038	0	C	T	100.0%	179.0 / NA	66	T110T (ACG→ACA)	unknown	Programmed cell death toxin MazF
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/39);  total (27/39)

CCCCGGTTAAATTAAACGAGGAGCAAAAAGAATTATTACGCAAACTGGAGGAAAGCCTGGAAGGACAAACCAAGCAACGTCCGAAATCCTCCAGCTTCTTAGATGGCGTAAAACGTTTCTTTGATGATCTGACGAAGTAAACCAGTTTAAAAACAGAAAAGTGCGGTAGAAATTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCCGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTACGCCAATCTAATGTTCTGGATTGATTGGCTACAACGCCACCTAATGTATCCAAACCATATCCCATTAAACTCACATAAAAGCCACCTTTTCTAGAAATTGAGGCTTCACCTTGCGTGATCGGGCAGACTAAAATTAATCCATATTGATTAAAACGTTTGGGGC  >  1/1328803‑1329270                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                         ccccGGTTAAATTAAACGAGGAGCAAAAAGAATTATTACGCAAACTGGAGGAAAGCCTGGAAGGACAAACCAAGCAACGTCCGAAATCCTCCAGCTTCTTAGATGGCGTAAAACGTTTCTTTGATGATCTGACGAAGTAAACCAGTTTAAAAACAGAAAAGTGCGGTAGAAATTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTgg                                                                                                                                                                                                                           <  1:793584/251‑1 (MQ=255)   ccGGTTAAATTAAACGAGGAGCAAAAAGAATTATTACGCAAACTGGAGGAAAGCCTGGAAGGACAAACCAAGCAACGTCCGAAATCCTCCAGCTTCTTAGATGGCGTAAAACGTTTCTTTGATGATCTGACGAAGTAAACCAGTTTAAAAACAGAAAAGTGCGGTAGAAATTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGAc                                                                                                                                                                                                                         <  1:866891/251‑1 (MQ=255)         aaattaaaCGAGGAGCAAAAAGAATTATTACGCAAACTGGAGGAAAGCCTGGAAGGACAAACCAAGCAACGTCCGAAATCCTCCAGCTTCTTAGATGGCGTAAAACGTTTCTTTGATGATCTGACGAAGTAAACCAGTTTAAAAACAGAAAAGTGCGGTAGAAATTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTc                                                                                                                                                                                                                      >  1:219892/1‑248 (MQ=255)            ttaaaCGAGGAGCAAAAAGAATTATTACGCAAACTGGAGGAAAGCCTGGAAGGACAAACCAAGCAACGTCCGAAATCCTCCAGCTTCTTAGATGGCGTAAAACGTTTCTTTGATGATCTGACGAAGTAAACCAGTTTAAAAACAGAAAAGTGCGGTAGAAATTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCAc                                                                                                                                                                                                                <  1:645621/251‑1 (MQ=255)            ttaaaCGAGGAGCAAAAAGAATTATTACGCAAACTGGAGGAAAGCCTGGAAGGACAAACCAAGCAACGTCCGAAATCCTCCAGCTTCTTAGATGGCGTAAAACGTTTCTTTGATGATCTGACGAAGTAAACCAGTTTAAAAACAGAAAAGTGCGGTAGAAATTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCAc                                                                                                                                                                                                                <  1:760866/251‑1 (MQ=255)                   aggagCAAAAAGAATTATTACGCAAACTGGAGGAAAGCCTGGAAGGACAAACCAAGCAACGTCCGAAATCCTCCAGCTTCTTAGATGGCGTAAAACGTTTCTTTGATGATCTGACGAAGGAAACCAGTTTAAAAACAGAAAAGTGCGGTAGAAATTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTc                                                                                                                                                                                                          <  1:35709/250‑1 (MQ=255)                              gAATTATTACGCAAACTGGAGGAAAGCCTGGAAGGACAAACCAAGCAACGTCCGAAATCCTCCAGCTTCTTAGATGGCGTAAAACGTTTCTTTGATGATCTGACGAAGTAAACCAGTTTAAAAACAGAAAAGTGCGGTAGAAATTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCt                                                                                                                                                                                                                              <  1:302368/219‑1 (MQ=255)                              gAATTATTACGCAAACTGGAGGAAAGCCTGGAAGGACAAACCAAGCAACGTCCGAAATCCTCCAGCTTCTTAGATGGCGTAAAACGTTTCTTTGATGATCTGACGAAGTAAACCAGTTTAAAAACAGAAAAGTGCGGTAGAAATTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGt                                                                                                                                                                                              >  1:685084/1‑251 (MQ=255)                                   attaCGCAAACTGGAGGAAAGCCTGGAAGGACAAACCAAGCAACGTCCGAAATCCTCCAGCTTCTTAGATGGCGTAAAACGTTTCTTTGATGATCTGACGAAGTAAACCAGTTTAAAAACAGAAAAGTGCGGTAGAAATTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGAc                                                                                                                                                                                                                         <  1:432759/219‑1 (MQ=255)                                   attaCGCAAACTGGAGGAAAGCCTGGAAGGACAAACCAAGCAACGTCCGAAATCCTCCAGCTTCTTAGATGGCGTAAAACGTTTCTTTGATGATCTGACGAAGTAAACCAGTTTAAAAACAGAAAAGTGCGGTAGAAATTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGAc                                                                                                                                                                                                                         >  1:242545/1‑219 (MQ=255)                                           aaCTGGAGGAAAGCCTGGAAGGACAAACCAAGCAACGTCCGAAATCCTCCAGCTTCTTAGATGGCGTAAAACGTTTCTTTGATGATCTGACGAAGTAAACCAGTTTAAAAACAGAAAAGTGCGGTAGAAATTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGAc                                                                                                                                                                                                                         <  1:66578/211‑1 (MQ=255)                                             cTGGAGGAAAGCCTGGAAGGACAAACCAAGCAACGTCCGAAATCCTCCAGCTTCTTAGATGGCGTAAAACGTTTCTTTGATGATCTGACGAAGTAAACCAGTTTAAAAACAGAAAAGTGCGGTAGAAATTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATc                                                                                                                                                                                                                               <  1:683898/203‑1 (MQ=255)                                             cTGGAGGAAAGCCTGGAAGGACAAACCAAGCAACGTCCGAAATCCTCCAGCTTCTTAGATGGCGTAAAACGTTTCTTTGATGATCTGACGAAGTAAACCAGTTTAAAAACAGAAAAGTGCGGTAGAAATTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAAt                                                                                                                                                                               <  1:433764/251‑1 (MQ=255)                                                gaggaAAGCCTGGAAGGACAAACCAAGCAACGTCCGAAATCCTCCAGCTTCTTAGATGGCGTAAAACGTTTCTTTGATGATCTGACGAAGTAAACCAGTTTAAAAACAGAAAAGTGCGGTAGAAATTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCg                                                                                                                                                                             <  1:505465/250‑1 (MQ=255)                                                       gCCTGGAAGGACAAACCAAGCAACGTCCGAAATCCTCCAGCTTCTTAGATGGCGTAAAACGTTTCTTTGATGATCTGACGAAGTAAACCAGTTTAAAAACAGAAAAGTGCGGTAGAAATTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTA                                                                                                                                                                     >  1:118341‑M1/1‑251 (MQ=255)                                                         cTGGAAGGACAAACCAAGCAACGTCCGAAATCCTCCAGCTTCTTAGATGGCGTAAAACGTTTCTTTGATGATCTGACGAAGTAAACCAGTTTAAAAACAGAAAAGTGCGGTAGAAATTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTAac                                                                                                                                                                   <  1:774463‑M1/251‑3 (MQ=255)                                                          tGGAAGGACAAACCAAGCAACGTCCGAAATCCTCCAGCTTCTTAGATGGCGTAAAACGTTTCTTTGATGATCTGACGAAGTAAACCAGTTTAAAAACAGAAAAGTGCGGTAGAAATTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTAacc                                                                                                                                                                  <  1:10645‑M1/251‑4 (MQ=255)                                                               ggACAAACCAAGCAACGTCCGAAATCCTCCAGCTTCTTAGATGGCGTAAAACGTTTCTTTGATGATCTGACGAAGTAAACCAGTTTAAAAACAGAAAAGTGCGGTAGAAATTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTAaccgttaa                                                                                                                                                             >  1:358672‑M1/1‑243 (MQ=255)                                                                  cAAACCAAGCAACGTCCGAAATCCTCCAGCTTCTTAGATGGCGTAAAACGTTTCTTTGATGATCTGACGAAGTAAACCAGTTTAAAAACAGAAAAGTGCGGTAGAAATTTTTGGTGTTTTTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACt                                                                                                                                                                                                           >  1:691905/1‑202 (MQ=255)                                                                   aaaCCAAGCAACGTCCGAAATCCTCCAGCTTCTTAGATGGCGTAAAACGTTTCTTTGATGATCTGACGAAGTAAACCAGTTTAAAAACAGAAAAGTGCGGTAGAAATTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTAaccgttaaaata                                                                                                                                                         >  1:292563‑M1/1‑239 (MQ=255)                                                                    aaCCAAGCAACGTCCGAAATCCTCCAGCTTCTTAGATGGCGTAAAACGTTTCTTTGATGATCTGACGAAGTAAACCAGTTTAAAAACAGAAAAGTGCGGTAGAAATTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTCCGGTTTCCACTTTCTTATTCGCTCGATTAcccgttaaattaa                                                                                                                                                        >  1:103856‑M1/1‑238 (MQ=255)                                                                     aCCAAGCAACGTCCGAAATCCTCCAGCTTCTTAGATGGCGTAAAACGTTTCTTTGATGATCTGACGAAGTAAACCAGTTTAAAAACAGAAAAGTGCGGTAGAAATTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCAc                                                                                                                                                                                        <  1:756408/218‑1 (MQ=255)                                                                                    aaaTCCTCCAGCTTCTTAGATGGCGTAAAACGTTTCTTTGATGATCTGACGAAGTAAACCAGTTTAAAAACAGAAAAGTGCGGTAGAAATTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTAaccgttaaaataaatttattatccactt                                                                                                                                         <  1:563517‑M1/250‑29 (MQ=255)                                                                                      aTCCTCCAGCTTCTTAGATGGCGTAAAACGTTTCTTTGATGATCTGACGAAGTAAACCAGTTTAAAAACAGAAAAGTGCGGTAGAAATTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTAaccgttaaaataaatttattatccacttggt                                                                                                                                      >  1:67611‑M1/1‑220 (MQ=255)                                                                                       tcctccAGCTTCTTAGATGGCGTAAAACGTTTCTTTGATGATCTGACGAAGTAAACCAGTTTAAAAACAGAAAAGTGCGGTAGAAATTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTAaccgttaaaataaatttattatccacttggtt                                                                                                                                     >  1:117214‑M1/1‑219 (MQ=255)                                                                                               cttcttAGATGGCGTAAAACGTTTCTTTGATGATCTGACGAAGTAAACCAGTTTAAAAACAGAAAAGTGCGGTAGAAATTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTAaccgttaaaataaatttattatccacttggtttaattc                                                                                                                               <  1:606547‑M1/249‑39 (MQ=255)                                                                                                ttcttAGATGGCGTAAAACGTTTCTTTGATGATCTGACGAAGTAAACCAGTTTAAAAACAGAAAAGTGCGGTAGAAATTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTAaccgttaaaataaatttattatccacttggtttaattctaa                                                                                                                            >  1:409677‑M1/1‑210 (MQ=255)                                                                                                ttcttAGATGGCGTAAAACGTTTCTTTGATGATCTGACGAAGTAAACCAGTTTAAAAACAGAAAAGTGCGGTAGAAATTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTAaccgttaaaataaatttattatcc                                                                                                                                             >  1:179375‑M1/1‑210 (MQ=255)                                                                                                          gCGTAAAACGTTTCTTTGATGATCTGACGAAGTAAACCAGTTTAAAAACAGAAAAGTGCGGTAGAAATTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATc                                                                                                                                                                                                                               >  1:111945/1‑142 (MQ=255)                                                                                                          gCGTAAAACGTTTCTTTGATGATCTGACGAAGTAAACCAGTTTAAAAACAGAAAAGTGCGGTAGAAATTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTAaccgttaaaata                                                                                                                                                         >  1:286056‑M1/1‑200 (MQ=255)                                                                                                            gTAAAACGTTTCTTTGATGATCTGACGAAGTAAACCAGTTTAAAAACAGAAAAGTGCGGTAGAAATTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAatat                                                                                                                                                                                                                                <  1:412259/139‑1 (MQ=255)                                                                                                                    tttCTTTGATGATCTGACGAAGTAAACCAGTTTAAAAACAGAAAAGTGCGGTAGAAATTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTAaccgttaaaataaatttattatccacttggtttaattctaaaaaatcacccgtatcaagat                                                                                                        <  1:845051‑M1/251‑62 (MQ=255)                                                                                                                    tttCTTTGATGATCTGACGAAGTAAACCAGTTTAAAAACAGAAAAGTGCGGTAGAAATTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTAaccgttaaaataaatttattatccacttggtttaattctaaaaaatcacccgtatcaagat                                                                                                        <  1:305959‑M1/251‑62 (MQ=255)                                                                                                                      tCTTTGATGATCTGACGAAGTAAACCAGTTTAAAAACAGAAAAGTGCGGTAGAAATTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTAaccgttaaaataaatttattatccac                                                                                                                                           >  1:797393‑M1/1‑188 (MQ=255)                                                                                                                            atgatCTGACGAAGTAAACCAGTTTAAAAACAGAAAAGTGCGGTAGAAATTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAAt                                                                                                                                                                               >  1:722880/1‑172 (MQ=255)                                                                                                                              gatCTGACGAAGTAAACCAGTTTAAAAACAGAAAAGTGCGGTAGAAATTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATg                                                                                                                                                                                                      <  1:153779/147‑1 (MQ=255)                                                                                                                                 cTGACGAAGTAAACCAGTTTAAAAACAGAAAAGTGCGGTAGAAATTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTAaccgttaaaataaatttattatccacttggtttaattctaaaaaatcacccgtatcaagatttagtgtatgaat                                                                                           <  1:823141‑M1/251‑75 (MQ=255)                                                                                                                                         gTAAACCAGTTTAAAAACAGAAAAGTGCGGTAGAAATTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCAc                                                                                                                                                                                        <  1:830718/150‑1 (MQ=255)                                                                                                                                                 gTTTAAAAACAGAAAAGTGCGGTAGAAATTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTAaccgttaaaataaatttattatccacttggtttaattctaaaaaatcacccgtatcaagatttagtgtatgaataaaatctttcggtaag                                                                           >  1:749965‑M1/1‑161 (MQ=255)                                                                                                                                                                       tAGAAATTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTAaccgttaaaataaatttattatccacttggtttaattctaaaaaatcacccgtatcaagatttagtgtatgaataaaatctttcggtaagcgaattgccttgctatttccc                                                      <  1:895600‑M1/250‑112 (MQ=255)                                                                                                                                                                           aaTTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTAaccgttaaaataaatttattatccacttggtttaattctaaaaaatcacccgtatcaagatttagtgtatgaataaaatctttcggtaagcgaattgc                                                                   >  1:682758‑M1/1‑135 (MQ=255)                                                                                                                                                                           aaTTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACACAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTAaccgttaaaataaatttattatccacttggtttaattctaaaaaatcacccgtatcaagatttagtgtatgaataaaatctttcggtaagcgaattgccttgctatttccccattg                                                 <  1:648143‑M1/251‑117 (MQ=255)                                                                                                                                                                             tttttGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTAaccgttaaaataaatttattatccacttggtttaattctaaaaaatcacccgtatcaagatttagtgtatgaataaaatctttcggtaagcgaattgccttgcta                                                            <  1:693573‑M1/238‑106 (MQ=255)                                                                                                                                                                              accaaaaatttctaccgtaCTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTAaccgttaaaataaatttattatccacttggtttaattctaaaaaatcacccgtatcaagatttagtgtatgaataaaatctttcggtaagcgaattgccttgctatttccccattgttt                                              <  1:583003‑M1/243‑120 (MQ=255)                                                                                                                                                                                   gtgtTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTAaccgttaaaataaatttattatccacttggtttaattctaaaaaatcacccgtatcaagatttagtgtatgaataaaatctttcggtaagcgaattgccttgctatttccccattgtttaattt                                         >  1:688795‑M1/1‑127 (MQ=255)                                                                                                                                                                                   gtgtTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTAaccgttaaaataaatttattatccacttggtttaattctaaaaaatcacccgtatcaagatttagtgtatgaataaaatctttcggtaagcgaattgccttgctatttccccattgtttaattt                                         >  1:621970‑M1/1‑127 (MQ=255)                                                                                                                                                                                   gtgtTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTAaccgttaaaataaatttattatccacttggtttaattctaaaaaatcacccgtatcaagatttagtgtatgaataaaatctttcggtaagcgaattgccttgctatttccccattgtttaattt                                         >  1:542853‑M1/1‑127 (MQ=255)                                                                                                                                                                                    tgtTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTAaccgttaaaataaatttattatccacttggtttaattctaaaaaatcacccgtatcaagatttagtgtatgaataaaatctttcggtaagcgaattgccttgctatttccccattgtttaattt                                         <  1:640369‑M1/250‑125 (MQ=255)                                                                                                                                                                                    tgtTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTAaccgttaaaataaatttattatccacttggtttaattctaaaaaatcacccgtatcaagatttagtgtatgaataaaatctttcggtaagcgaattgccttgctatttccccattgtttaattt                                         <  1:550889‑M1/250‑125 (MQ=255)                                                                                                                                                                                         cTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTAaccgttaaaataaatttattatccacttggtttaattctaaaaaatcacccgtatcaagatttagtgtatgaataaaatctttcggtaagcgaattgccttgctatttccccattgtttaatttctagtc                                   <  1:875557‑M1/251‑131 (MQ=255)                                                                                                                                                                                          tACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTAaccgttaaaataaatttattatccacttggtttaattctaaaaaatcacccgtatcaagatttagtgtatgaataaaatctttcggtaagcgaattgccttgctatttccccattgtttaatttctagtct                                  <  1:336422‑M1/251‑132 (MQ=255)                                                                                                                                                                                            ccGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTAaccgttaaaataaatttattatccacttggtttaattctaaaaaatcacccgtatcaagatttagtgtatgaataaaatctttcggtaagcgaattgccttgctatttccccattgtttaatttctagtcttg                                >  1:506909‑M1/1‑118 (MQ=255)                                                                                                                                                                                                 cTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTAaccgttaaaataaatttattatccacttggtttaattctaaaaaatcacccgtatcaagatttagtgtatgaataaaatctttcggtaagcgaattgccttgctatttccccattgtttaatttc                                        <  1:321554‑M1/238‑126 (MQ=255)                                                                                                                                                                                                 cTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTAaccgttaaaataaatttattatccacttggtttaattctaaaaaatcacccgtatcaagatttagtgtatgaataaaatctttcggtaagcgaattgccttgctatttccccattgtttaatt                                          <  1:478891‑M1/236‑124 (MQ=255)                                                                                                                                                                                                 cTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTAaccgttaaaataaatttattatccacttg                                                                                                                                        <  1:11864‑M1/142‑30 (MQ=255)                                                                                                                                                                                                           gCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTAaccgttaaaataaatttattatccacttggtttaattctaaaaaatcacccgtatcaagatttagtgtatgaataaaatctttcggtaagcgaattgccttgctatttccccattgtttaatttctagtcttgtattcattacctca                  >  1:8820‑M1/1‑103 (MQ=255)                                                                                                                                                                                                           gCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTAaccgttaaaataaatttattatccacttggtttaattctaaaaaatcacccgtatcaagatttagtgtatgaataaaatctttcggtaagcgaattgccttgctatttccccattgtttaatttctagtcttgtattcattacctca                  >  1:798924‑M1/1‑103 (MQ=255)                                                                                                                                                                                                           gCATTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTAaccgttaaaataaatttattatccacttggtttaattctaaaaaatcacccgtatcaagatttagtgtatgaataaaatctttcggtaagcgaattgccttgctatttccccattgtttaatttctagtcttgtattcattacc                     >  1:897658‑M1/1‑103 (MQ=255)                                                                                                                                                                                                             aTTATCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTAaccgttaaaataaatttattatccacttggtttaattctaaaaaatcacccgtatcaagatttagtgtatgaataaaatctttcggtaagcgaattgccttgctatttccccattgtttaatttctagtcttgtattcattacctcacct               <  1:751021‑M1/251‑151 (MQ=255)                                                                                                                                                                                                                aTCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTAaccgttaaaataaatttattatccacttggtttaattctaaaaaatcacccgtatcaagatttagtgtatgaataaaatc                                                                                     <  1:347316‑M1/178‑81 (MQ=255)                                                                                                                                                                                                                aTCTTCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTAaccgttaaaataaatttattatccacttggtttaattctaaaaaatcacccgtatca                                                                                                            >  1:124962‑M1/1‑98 (MQ=255)                                                                                                                                                                                                                   ttCGCCCTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTAaccgttaaaataaatttattatccacttggtttaattctaaaaaatcacccgtatcaagatttagtgtatgaataaaatctttcggtaagcgaattgccttgctatttccccattgtttaatttctagtcttgtattcattacctcacctcaacgt         <  1:63707‑M1/251‑157 (MQ=255)                                                                                                                                                                                                                       cccTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTAaccgttaaaataaatttattatccacttggtttaattctaaaaaatcacccgtatcaagatttagtgtatgaa                                                                                            <  1:506530‑M1/164‑74 (MQ=255)                                                                                                                                                                                                                        ccTCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTAaccgttaaaataaatttattatccacttggtttaattctaaaaaatcacccgtatcaagatttagtgtatgaataaaatctttcggtaagcgaattgccttgctatttccccattgtttaatttctagtcttgtattcattacctcacctcaacgtatcat    <  1:656391‑M1/251‑162 (MQ=255)                                                                                                                                                                                                                          tCAATCATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTAaccgttaaaataaatttattatccacttggtttaattctaaaaaatcacccgtatcaagatttagtgtatgaataaaatctttcggtaagcgaattgccttgctatttccccattgtttaatttctagtcttgtattcattacctcacctcaacgtatcattt  <  1:92083‑M1/251‑164 (MQ=255)                                                                                                                                                                                                                               cATTAACGATTGCTGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCAc                                                                                                                                                                                        >  1:27179/1‑64 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                         CCCCGGTTAAATTAAACGAGGAGCAAAAAGAATTATTACGCAAACTGGAGGAAAGCCTGGAAGGACAAACCAAGCAACGTCCGAAATCCTCCAGCTTCTTAGATGGCGTAAAACGTTTCTTTGATGATCTGACGAAGTAAACCAGTTTAAAAACAGAAAAGTGCGGTAGAAATTTTTGGTGTTTCTACCGTACTTTTTATTCGCATTATCTTCGCCCTCAATCATTAACGATTGCCGTGATAATATCTTGGACTTCCTGCACTAACTCAATGGGTGCAGTTTCCACTTTCTTAATCGCTCGATTACGCCAATCTAATGTTCTGGATTGATTGGCTACAACGCCACCTAATGTATCCAAACCATATCCCATTAAACTCACATAAAAGCCACCTTTTCTAGAAATTGAGGCTTCACCTTGCGTGATCGGGCAGACTAAAATTAATCCATATTGATTAAAACGTTTGGGGC  >  1/1328803‑1329270

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑