Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,324,074	T→C	K374R (AAA→AGA)	unknown ←	GTP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,324,074	0	T	C	100.0%	120.7 / NA	43	K374R (AAA→AGA)	unknown	GTP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/24);  total (19/24)

AATTTCAGCTGGCCTTTTTCCAATACGAGATCCAATTTTACTTGTTTACCGTCTTCGACCAATACACCTTGCAGAACACCGCTTTTCACACCTTCATTCACTTGTGTGGCTGCCATTTTCATTGCTTGCTCTCTATCCGGGCTTTTCAATTGCAGTGCGTTCGCCATTTCTTCGATTAAAGCATCTTTGTTCGCATCCACTTTGAAAATAAAGTGATTAAATAGATTGAAGATTTTTCCTTTGCTAATAGAATTTTCAAAATCAGAGCTTGCAATAGAAATATCTAAATTTGAGGCAATTTTACCGACTTGATTAGTTAATGTCGGACTAAACACAAATTGCGGTTGAGTATCAAGGAATTTCTTCGCTGCCTGTAATTTGGCTTCGGCAAATTCTTCTTGCTGCTTTTTCGCCGCCTCGGTATAAACCTGAATTAATTGATTAAAAGTTTCACCGTCAACGTGATTAAAAGCAGAATCAATAG  >  1/1323834‑1324317                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    aaTTTCAGCTGGCCTTTTTCCAATACGAGATCCAATTTTACTTGTTTACCGTCTTCGACCAATACACCTTGCAGAACACCGCTTTTCACACCTTCATTCACTTGTGTGGCTGCCATTTTCATTGCTTGCTCTCTATCCGGGCTTTTCAATTGCAGTGCGTTCGCCATTTCTTCGATTAAAGCATCTTTGTTCGCATCCACTTTGAAAATAAAGTGATTAAATAGATTGAAGATTTTTCCTCTGCTAATAGa                                                                                                                                                                                                                                           <  1:820154/251‑1 (MQ=255)  aTTTCAGCTGGCCTTTTTCCAATACGAGATCCAATTTTACTTGTTTACCGTCTTCGACCAATACACCTTGCAGAACACCGCTTTTCACACCTTCATTCACTTGTGTGGCTGCCATTTTCATTGCTTGCTCTCTATCCGGGCTTTTCAATTGCAGTGCGTTCGCCATTTCTTCGATTAAAGCATCTTTGTTCGCATCCACTTTGAAAATAAAGTGATTAAATAGATTGAAGATTTTTCCTCTGCTAATAGaa                                                                                                                                                                                                                                          >  1:240012/1‑251 (MQ=255)         cTGGCCTTTTTCCAATACGAGATCCAATTTTACTTGTTTACCGTCTTCGACCAATACACCTTGCAGAACACCGCTTTTCACACCTTCATTCACTTGTGTGGCTGCCATTTTCATTGCTTGCTCTCTATCCGGGCTTTTCAATTGCAGTGCGTTCGCCATTTCTTCGATTAAAGCATCTTTGTTCGCATCCACTTTGAAAATAAAGTGATTAAATAGATTGAAGATTTTTCCTCTGCTAATAGAATTTTCa                                                                                                                                                                                                                                    <  1:637410/250‑1 (MQ=255)                  ttCCAATACGAGATCCAATTTTACTTGTTTACCGTCTTCGACCAATACACCTTGCAGAACACCGCTTTTCACACCTTCATTCACTTGTGTGGCTGCCATTTTCATTGCTTGCTCTCTATCCGGGCTTTTCAATTGCAGTGCGTTCGCCATTTCTTCGATTAAAGCATCTTTGTTCGCATCCACTTTGAAAATAAAGTGATTAAATAGATTGAAGATTTTTCCTCTGCTAATAGAATTTTCAAAATCagag                                                                                                                                                                                                                           >  1:508412/1‑250 (MQ=255)                         aCGAGATCCAATTTTACTTGTTTACCGTCTTCGACCAATACACCTTGCAGAACACCGCTTTTCACACCTTCATTCACTTGTGTGGCTGCCATTTTCATTGCTTGCTCTCTATCCGGGCTTTTCAATTGCAGTGCGTTCGCCATTTCTTCGATTAAAGCATCTTTGTTCGCATCCACTTTGAAAATAAAGTGATTAAATAGATTGAAGATTTTTCCTCTGCTAATAGAATTTTCAAAATCAGAGCTTGCAAt                                                                                                                                                                                                                   <  1:341531/251‑1 (MQ=255)                              aTCCAATTTTACTTGTTTACCGTCTTCGACCAATACACCTTGCAGAACACCGCTTTTCACACCTTCATTCACTTGTGTGGCTGCCATTTTCATTGCTTGCTCTCTATCCGGGCTTTTCAATTGCAGTGCGTTCGCCATTTCTTCGATTAAAGCATCTTTGTTCGCATCCACTTTGAAAATAAAGTGATTAAATAGATTGAAGATTTTTCCTCTGCTAATAGAATTTTCAAAATCAGAGCTTGCAATAGaaa                                                                                                                                                                                                              <  1:403173/251‑1 (MQ=255)                              aTCCAATTTTACTTGTTTACCGTCTTCGACCAATACACCTTGCAGAACACCGCTTTTCACACCTTCATTCACTTGTGTGGCTGCCATTTTCATTGCTTGCTCTCTATCCGGGCTTTTCAATTGCAGTGCGTTCGCCATTTCTTCGATTAAAGCATCTTTGTTCGCATCCACTTTGAAAATAAAGTGATTAAATAGATTGAAGATTTTTCCTCTGCTAATAGAATTTTCAAAATCAGAGCTTGCAATAGaaa                                                                                                                                                                                                              <  1:698526/251‑1 (MQ=255)                                                   gTCTTCGACCAATACACCTTGCAGAACACCGCTTTTCACACCTTCATTCACTTGTGTGGCTGCCATTTTCATTGCTTGCTCTCTATCCGGGCTTTTCAATTGCAGTGCGTTCGCCATTTCTTCGATTAAAGCATCTTTGTTCGCATCCACTTTGAAAATAAAGTGATTAAATAGATTGAAGATTTTTCCTCTGCTAATAGAATTTTCAAAATCAGAGCTTGCAATAGAAATATCTAAATTTGAGGCAAttt                                                                                                                                                                                         >  1:786655/1‑251 (MQ=255)                                                          aCCAATACACCTTGCAGAACACCGCTTTTCACACCTTCATTCACTTGTGTGGCTGCCATTTTCATTGCTTGCTCTCTATCCGGGCTTTTCAATTGCAGTGCGTTCGCCATTTCTTCGATTAAAGCATCTTTGTTCGCATCCACTTTGAAAATAAAGTGATTAAATAGATTGAAGATTTTTCCTCTGCTAATAGAATTTTCAAAATCAGAGCTTGCAATAGAAATATCTAAATTTGAGGCAATTTTACCGa                                                                                                                                                                                   >  1:243830/1‑250 (MQ=255)                                                           ccAATACACCTTGCAGAACACCGCTTTTCACACCTTCATTCACTTGTGTGGCTGCCATTTTCATTGCTTGCTCTCTATCCGGGCTTTTCAATTGCAGTGCGTTCGCCATTTCTTCGATTAAAGCATCTTTGTTCGCATCCACTTTGAAAATAAAGTGATTAAATAGATTGAAGATTTTTCCTCTGCTAATAGAATTTTCAAAATCAGAGCTTGCAATAGAAATATCTAAATTTGAGGCAATTTTACCg                                                                                                                                                                                    <  1:464012/248‑1 (MQ=255)                                                                     ttGCAGAACACCGCTTTTCACACCTTCATTCACTTGTGTGGCTGCCATTTTCATTGCTTGCTCTCTATCCGGGCTTTTCAATTGCAGTGCGTTCGCCATTTCTTCGATTAAAGCATCTTTGTTCGCATCCACTTTGAAAATAAAGTGATTAAATAGATTGAAGATTTTTCCTCTGCTAATAGAATTTTCAAAATCAGAGCTTGCAATAGAAATATCTAAATTTGAGGCAATTTTACCGACTTGATTAGTTa                                                                                                                                                                       >  1:866301/1‑251 (MQ=255)                                                                                        acacCTTCATTCACTTGTGTGGCTGCCATTTTCATTGCTTGCTCTCTATCCGGGCTTTTCAATTGCAGTGCGTTCGCCATTTCTTCGATTAAAGCATCTTTGTTCGCATCCACTTTGAAAATAAAGTGATTAAATAGATTGAAGATTTTTCCTCTGCTAATAGAATTTTCAAAATCAGAGCTTGCAATAGAAATATCTAAATTTGAGGCAATTTTACCGACTTGATTAGTTAATGTCGGACTAAACACaaa                                                                                                                                                    <  1:395306/251‑1 (MQ=255)                                                                                              tcattcaCTTGTGTGGCTGCCATTTTCATTGCTTGCTCTCTATCCGGGCTTTTCAATTGCAGTGCGTTCGCCATTTCTTCGATTAAAGCATCTTTGTTCGCATCCACTTTGAAAATAAAGTGATTAAATAGATTGAAGATTTTTCCTCTGCTAATAGAATTTTCAAAATCAGAGCTTGCAATAGAAATATCTAAAtt                                                                                                                                                                                                    >  1:98839/1‑197 (MQ=255)                                                                                              tcattcaCTTGTGTGGCTGCCATTTTCATTGCTTGCTCTCTATCCGGGCTTTTCAATTGCAGTGCGTTCGCCATTTCTTCGATTAAAGCATCTTTGTTCGCATCCACTTTGAAAATAAAGTGATTAAATAGATTGAAGATTTTTCCTCTGCTAATAGAATTTTCAAAATCAGAGCTTGCAATAGAAATATCTAAATTTGAGGCAATTTTACCGACTTGATTAg                                                                                                                                                                          <  1:149415/223‑1 (MQ=255)                                                                                                  tcaCTTGTGTGGCTGCCATTTTCATTGCTTGCTCTCTATCCGGGCTTTTCAATTGCAGTGCGTTCGCCATTTCTTCGATTAAAGCATCTTTGTTCGCATCCACTTTGAAAATAAAGTGATTAAATAGATTGAAGATTTTTCCTCTGCTAATAGAATTTTCAAAATCAGAGCTTGCAATAGAAATATCTAAATTTGAGGCAATTTTACCGACTTGATTAGTTAATGTCGGACTAAACACAAATTGCGGTTGa                                                                                                                                          >  1:692513/1‑251 (MQ=255)                                                                                                   caCTTGTGTGGCCGCCATTTTCATTGCTTGCTCTCTATCCGGGCTTTTCAATTGCAGTGCGTTCGCCATTTCTTCGATTAAAGCATCTTTGTTCGCATCCACTTTGAAAATAAAGTGATTAAATAGATTGAAGATTTTTCCTCTGCTAATAGAATTTTCAAAATCAGAGCTTGCAATAGaaa                                                                                                                                                                                                              <  1:451667/182‑1 (MQ=255)                                                                                                                   aTTTTCATTGCTTGCTCTCTATCCGGGCTTTTCAATTGCAGTGCGTTCGCCATTTCTTCGATTAAAGCATCTTTGTTCGCATCCACTTTGAAAATAAAGTGATTAAATAGATTGAAGATTTTTCCTCTGCTAATAGAATTTTCAAAATCAGAGCTTGCAATAGAAATATCTAAATTTGAGGCAATTTTACCGACTTGATTAGTTAATGTCGGACTAAACACAAATTGCGGTTGAGTATCAAGGAATttctt                                                                                                                         <  1:501407/251‑1 (MQ=255)                                                                                                                   aTTTTCATTGCTTGCTCTCTATCCGGGCTTTTCAATTGCAGTGCGTTCGCCATTTCTTCGATTAAAGCATCTTTGTTCGCACCCACTTTGAAAATAAAGTGATTAAATAGATTGAAGATTTTTCCTCTGCTAATAGAATTTTCAAAATCAGAGCTTGCAATAGAAATATCTAAATTTGAGGCAATTTTACCGACTTGATTAGTTAATGTCGGACTAAACACAAATTGCGGTTGAGTATCAAGGAATttctt                                                                                                                         <  1:438356/251‑1 (MQ=255)                                                                                                                     tttCATTGCTTGCTCTCTATCCGGGCTTTTCAATTGCAGTGCGTTCGCCATTTCTTCGATTAAAGCATCTTTGTTCGCATCCACTTTGAAAATAAAGTGATTAAATAGATTGAAGATTTTTCCTCTGCTAATAGAATTTTCAAAATCAGAGCTTGCAATAGAAATATCTAAATTTGAGGCAATTTTACCGACTTGATTAGTTAATGTCGGACTAAACACAAATTg                                                                                                                                                 <  1:817666/225‑1 (MQ=255)                                                                                                                      ttCATTGCTTGCTCTCTATCCGGGCTTTTCAATTGCAGTGCGTTCGCCATTTCTTCGATTAAAGCATCTTTGTTCGCATCCACTTTGAAAATAAAGTGATTAAATAGATTGAAGATTTTTCCTCTGCTAATAGAATTTTCAAAATCAGAGCTTGCAATAGAAATATCTAAATTTGAGGCAATTTTACCGGCTTGATTATTTAATGTCGGACTAAACACAAATTGCGATTGAGTATCAAGGAATTTCTTCGc                                                                                                                      <  1:800140/251‑1 (MQ=255)                                                                                                                                gctCTCTATCCGGGCTTTTCAATTGCAGTGCGTTCGCCATTTCTTCGATTAAAGCATCTTTGTTCGCATCCACTTTGAAAATAAAGTGATTAAATAGATTGAAGATTTTTCCTCTGCTAATAGAATTTTCAAAATCAGAGCTTGCAATAGAAATATCTAAATTTGAGGCCATTTTACCGACTTGATTAGTTAATGTCGGACTAAACACAAATTGCGGTTGAGTATCAAGGAATTTCTTCGCTGCCTGTAAt                                                                                                            >  1:439756/1‑251 (MQ=255)                                                                                                                                 ctctctATCCGGGCTTTTCAATTGCAGTGCGTTCGCCATTTCTTCGATTAAAGCATCTTTGTTCGCATCCACTTTGAAAATAAAGTGATTAAATAGATTGAAGATTTTTCCTCTGCTAATAGAATTTTCAAAATCAGAGCTTGCAATAGAAATATCTAAATTTGAGGCAATTTTACCGACTTGATTAGTTAATGTCGGACTAAACACAAATTGCGGTTGAGTATCAAgg                                                                                                                                 <  1:260170/229‑1 (MQ=255)                                                                                                                                 ctctctATCCGGGCTTTTCAATTGCAGTGCGTTCGCCATTTCTTCGATTAAAGCATCTTTGTTCGCATCCACTTTGAAAATAAAGTGATTAAATAGATTGAAGATTTTTCCTCTGCTAATAGAATTTTCAAAATCAGAGCTTGCAATAGAAATATCTAAATTTGAGGCAATTTTACCGACTTGATTAGTTAATGTCGGACTAAACACAAATTGCGGTTGAGTATCAAGGAATTTCTTCGCTGCCTGTAAt                                                                                                            <  1:583510/250‑1 (MQ=255)                                                                                                                                   ctctATCCGGGCTTTTCAATTGCAGTGCGTTCGCCATTTCTTCGATTAAAGCATCTTTGTTCGCATCCACTTTGAAAATAAAGTGATTAAATAGATTGAAGATTTTTCCTCTGCTAATAGAATTTTCAAAATCAGAGCTTGCAATAGaaa                                                                                                                                                                                                              >  1:848467/1‑150 (MQ=255)                                                                                                                                              cTTTTCAATTGCAGTGCGTTCGCCATTTCTTCGATTAAAGCATCTTTGTTCGCATCCACTTTGAAAATAAAGTGATTAAATAGATTGAAGATTTTTCCTCTGCTAATAGAATTTTCAAAATCAGAGCTTGCAATAGAAATATCTAAATTTGAGGCAATTTTACCGACTTGATTAGTTAATGTCGGACTAAACACAAATTGCGGTTGAGTATCAAGGAATTTCTTCGCTGCCTGTAATTTGGCTTCGGCaaa                                                                                              >  1:56937/1‑251 (MQ=255)                                                                                                                                                   cAATTGCAGTGCGTTCGCCATTTCTTCGATTAAAGCATCTTTGTTCGCATCCACTTTGAAAATAAAGTGATTAAATAGATTGAAGATTTTTCCTCTGCTAATAGAATTTTCAAAATCAGAGCTTGCAATAGAAATATCTAAATTTGAGGCAATTTTACCGACTTGATTAGTTAATGTCGGACTAAACACAAATTGCGGTTGAGTATCAAGGAATTTCTTCGCTGCCTGTAATTTGGCTTCGGCAAAttctt                                                                                         >  1:660737/1‑251 (MQ=255)                                                                                                                                                                  cGCCATTTCTTCGATTAAAGCATCTTTGTTCGCATCCACTTTGAAAATAAAGTGATTAAATAGATTGAAGATTTTTCCTCTGCTAATAGAATTTTCAAAATCAGAGCTTGCAATAGAAATATCTAAATTTGAGGCAATTTTACCGACTTGAtt                                                                                                                                                                            >  1:861241/1‑153 (MQ=255)                                                                                                                                                                    ccATTTCTTCGATTAAAGCATCTTTGTTCGCATCCACTTTGAAAATAAAGTGATTAAATAGATTGAAGATTTTTCCTCTGCTAATAGAATTTTCAAAATCAGAGCTTGCAATAGAAATATCTAAATTTGAGGCAATTTTACCGACTTGATTAGTTAATGTCGGACTAAACACAAATTGCGGTTGAGTATCAAGGAATTTCTTCGCTGCCTGTAATTTGGCTTCGGCAAATTCTTCTTGCTGCTTTTTcgc                                                                         >  1:203671/1‑250 (MQ=255)                                                                                                                                                                                   aaGCATCTTTGTTCGCATCCACTTTGAAAATAAAGTGATTAAATAGATTGAAGATTTTTCCTCTGCTAATAGAATTTTCAAAATCAGAGCTTGCAATAGAAATATCTAAATTTGAGGCAATTTTACCGACTTGATTAGTTAATGTCGGACTAAACACAAATTGCGGTTGAGTATCAAGGAATTTCTTCGCTGCCTGTAATTTGGCTTCGGCAAATTCTTCTTGCTGCTTTTTCGCCGCCTCGGTATAAAcc                                                         <  1:174657/251‑1 (MQ=255)                                                                                                                                                                                   aaGCATCTTTGTTCGCATCCACTTTGAAAATAAAGTGATTAAATAGATTGAAGATTTTTCCTCTGCTAATAGAATTTTCAAAATCAGAGCTTGCAATAGAAATATCTAAATTTGAGGCAATTTTACCGACTTGATTAGTTAATGTCGGACTAAACACAAATTGCGGTTGAGTATCAAGGAATTTCTTCGCTGCCTGTAATTTGGCTTCGGCAAATTCTTCTTGCTGCTTTTTCGCCGCCTCGGTATAAAcc                                                         <  1:458033/251‑1 (MQ=255)                                                                                                                                                                                     gCATCTTTGTTCGCATCCACTTTGAAAATAAAGTGATTAAATAGATTGAAGATTTTTCCTCTGCTAATAGAATTTTCAAAATCAGAGCTTGCAATAGAAATATCTAAATTTGAGGCAATTTTACCGACTTGATTAGTTAATGt                                                                                                                                                                   <  1:747770/143‑1 (MQ=255)                                                                                                                                                                                        tCTTTGTTCGCATCCACTTTGAAAATAAAGTGATTAAATAGATTGAAGATTTTTCCTCTGCTAATAGAATTTTCAAAATCAGAGCTTGCAATAGAAATATCTAAATTTGAGGCAATTTTACCGACTTGATTAGTTAATGTCGGACTAAACACAAATTGCGGTTGAGTATCAAGGAATTTCTTCGCTGCCTGTAATTTGGCTTCGGCAAATTCTTCTTGCTGCTTTTTCGCCGCCTCGGTATAAACCTGaat                                                    >  1:428446/1‑251 (MQ=255)                                                                                                                                                                                                            gAAAATAAAGTGATTAAATAGATTGAAGATTTTTCCTCTGCTAATAGAATTTTCAAAATCAGAGCTTGCAATAGAAATATCTAAATTTGAGGCAATTTTACCGACTTGATTAGTTAATGTCGGACTAAACACAAATTGCGGTTGAGTATCAAGGAATTTCTTCGCTGCCTGTAATTTGGCTTCGGCAAAttcttc                                                                                        <  1:741444/195‑1 (MQ=255)                                                                                                                                                                                                                      tGATTAAATAGATTGAAGATTTTTCCTCTGCTAATAGAATTTTCAAAATCAGAGCTTGCAATAGAAATATCTAAATTTGAGGCAATTTTACCGACTTGATTAGTTAATGTCGGACTAAACACAAATTGCGGTTGAGTATCAAgg                                                                                                                                 <  1:549106/144‑1 (MQ=255)                                                                                                                                                                                                                      tGATTAAATAGATTGAAGATTTTTCCTCTGCTAATAGAATTTTCAAAATCAGAGCTTGCAATAGAAATATCTAAATTTGAGGCAATTTTACCGACTTGATTAGTTAATGTCGGACTAAACACAAATTGCGGTTGAGTATCAAGGAATTTCTTCGCTGCCTGTAATTTGGCTTCGGCAAATTCTTCTTGCTGCTTTTTCGCCGCCTCGGTATAAACCTGAATTAATTGATTAAAAGTTTCACCGTCAACGTg                      >  1:99095/1‑251 (MQ=255)                                                                                                                                                                                                                       gATTAAATAGATTGAAGATTTTTCCTCTGCTAATAGAATTTTCAAAATCAGAGCTTGCAATAGAAATATCTAAATTTGAGGCAATTTTACCGACTTGATTAGTTAATGTCGGACTAAACACAAATTGCGGTTGAGTATCAAGGAATTTCTTCGCTGCCTGTAAt                                                                                                            <  1:723065/164‑1 (MQ=255)                                                                                                                                                                                                                       gATTAAATAGATTGAAGATTTTTCCTCTGCTAATAGAATTTTCAAAATCAGAGCTTGCAATAGAAATATCTAAATTTGAGGCAATTTTACCGACTTGATTAGTTAATGTCGGACTAAACACAAATTGCGGTTGAGTATCAAGGAATTTCTTCGCTGCCTGTAATTTGGCTTCGGCAAATTCTTCTTGCTGCTTTTTCGCCGCCTCGGTATAAACCTGaattaatt                                               >  1:64919/1‑225 (MQ=255)                                                                                                                                                                                                                       gATTAAATAGATTGAAGATTTTTCCTCTGCTAATAGAATTTTCAAAATCAGAGCTTGCAATAGAAATATCTAAATTTGAGGCAATTTTACCGACTTGATTAGTTAATGTCGGACTAAACACAAATTGCGGTTGAGTATCAAGGAATTTCTTCGCTGCCTGTAATTTGGCTTCGGCAAATTCTTCTTGCTGCTTTTTCGCCGCCTCGGTATAAACCTGAATTAATTg                                              <  1:539969/226‑1 (MQ=255)                                                                                                                                                                                                                                gATTGAAGATTTTTCCTCTGCTAATAGAATTTTCAAAATCAGAGCTTGCAATAGAAATATCTAAATTTGAGGCAATTTTACCGACTTGATTAGTTAATGTCGGACTAAACACAAATTGCGGTTGAGTATCAAGGAATTTCTTCGCTGCCTGTAATTTGGCTTCGGCAAATTCTTCTTGCTGCTTTTTCGCCGCCTCGGTATAAACCTGAATTAATTGATTAAAAGTTTCACCGTCAACGTGATTAAAAGCa            >  1:547270/1‑251 (MQ=255)                                                                                                                                                                                                                                 aTTGAAGATTTTTCCTCTGCTAATAGAATTTTCAAAATCAGAGCTTGCAATAGAAATATCTAAATTTGAGGCAATTTTACCGACTTGATTAGTTAATGTCGGACTAAACACAAATTGCGGTTGAGTATCAAGGAATTTCTTCGCTGCCTGTAATTTGGCTTCGGCAAATTCTTCTTGCTGCTTTTTCGCCGCCTCGGTATAAACCTGAATTAATTGATTAAAAGTTTCACCGTCAACGTGATTAAAAGc             <  1:685685/249‑1 (MQ=255)                                                                                                                                                                                                                                       gATTTTTCCTCTGCTAATAGAATTTTCAAAATCAGAGCTTGCAATAGAAATATCTAAATTTGAGGCAATTTTACCGACTTGATTAGTTAATGTCGGACTAAACACAAATTGCGGTTGAGTATCAAGGAATTTCTTCGCTGCCTGTAATTTGGCTTCGGCAAATTCTTCTTGCTGCTTTTTCGCCGCCTCGGTATAAACCTGAATTAATTGATTAAAAGTTTCACCGTCAACGTGATTAAAAGCAGAATCaa     >  1:455554/1‑251 (MQ=255)                                                                                                                                                                                                                                         tttttCCTCTGCTAATAGAATTTTCAAAATCAGAGCTTGCAATAGAAATATCTAAATTTGAGGCAATTTTACCGACTTGATTAGTTAATGTCGGACTAAACACAAATTGCGGTTGAGTATCAAGGAATTTCTTCGCTGCCTGTAATTTGGCTTCGGCAAATTCTTCTTGCTGCTTTTTCGCCGCCTCGGTATAAACCTGAATTAATTGATTAAAAGTTTCACCGTCAACGTGATTAAAAGCAGAATCAATa   >  1:182445/1‑251 (MQ=255)                                                                                                                                                                                                                                          ttttCCTCTGCTAATAGAATTTTCAAAATCAGAGCTTGCAATAGAAATATCTAAATTTGAGGCAATTTTACCGACTTGATTAGTTAATGTCGGACTAAACACAAATTGCGGTTGAGTATCAAGGAATTTCTTCGCTGCCTGTAATTTGGCTTCGGCAAATTCTTCTTGCTGCTTTTTCGCCGCCTCGGTATAAACCTGAATTAATTGATTAAAAGTTTCACCGTCAACGTGATTAAAAGCAGAATCAATAg  <  1:582676/251‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                    AATTTCAGCTGGCCTTTTTCCAATACGAGATCCAATTTTACTTGTTTACCGTCTTCGACCAATACACCTTGCAGAACACCGCTTTTCACACCTTCATTCACTTGTGTGGCTGCCATTTTCATTGCTTGCTCTCTATCCGGGCTTTTCAATTGCAGTGCGTTCGCCATTTCTTCGATTAAAGCATCTTTGTTCGCATCCACTTTGAAAATAAAGTGATTAAATAGATTGAAGATTTTTCCTTTGCTAATAGAATTTTCAAAATCAGAGCTTGCAATAGAAATATCTAAATTTGAGGCAATTTTACCGACTTGATTAGTTAATGTCGGACTAAACACAAATTGCGGTTGAGTATCAAGGAATTTCTTCGCTGCCTGTAATTTGGCTTCGGCAAATTCTTCTTGCTGCTTTTTCGCCGCCTCGGTATAAACCTGAATTAATTGATTAAAAGTTTCACCGTCAACGTGATTAAAAGCAGAATCAATAG  >  1/1323834‑1324317

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑