Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,320,487	C→T	*152* (TAG→TAA)	unknown ←	Outer membrane lipoprotein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,320,487	0	C	T	100.0%	151.7 / NA	55	*152* (TAG→TAA)	unknown	Outer membrane lipoprotein
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (27/28);  total (27/28)

TCCTGATCGCTAATCACGGAAGCATCGCCTAACAAGGCTTCCAGCTCTTCATAACGTTCTTTTAAGCTATCTAATTTATTGATAATAGAGGGTTTCATTATTTGACCTTCAGAAGGAAAATAGAAAAGGAAATAAAAAAATCGGAGCATTCTAGCACAGAATGAAAATGTTGTTGAAGTTTAGGCTGTAAAAAAGGCTTTCGGATTTCGCCAAAAGCCTTTTAGGAATAGGTAGCACCTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGTTAGGTTCGGCTTTTTGCACTACAACAATTTCTTTTCCGTCATCTTTTTTGATTACCATTTCCAAGGCGCTAACTTGGCTGACTTTTTCTTCAACCTTACTGCCGGTAACTGCACCGGCAATTGCTCCAACAGCCGTTGCAAGCGCTCTACCTGTACCACCACCGATTGCAGAT  >  1/1320250‑1320724                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                              tCCTGATCGCTAATCACGGAAGCATCGCCTAACAAGGCTTCCAGCTCTTCATAACGTTCTTTTAAGCTATCTAATTTATTGATAATAGAGGGTTTCATTATTTGACCTTCAGAAGGAAAATAGAAAAGGAAATAAAAAAATCGGAGCATTCTAGCACAGAATGAAAATGTTGTTGAAGTTTAGGCTGTAAAAAAGGCTTTCGGATTTCGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGaaa                                                                                                                                                                                                                                  <  1:232685/250‑1 (MQ=255)            aaTCACGGAAGCATCGCCTAACAAGGCTTCCAGCTCTTCATAACGTTCTTTTAAGCTATCTAATTTATTGATAATAGAGGGTTTCATTATTTGACCTTCAGAAGGAAAATAGAAAAGGAAATAAAAAAATCGGAGCATTCTAGCACAGAATGAAAATGTTGTTGAAGTTTAGGCTGTAAAAAAGGCTTTCGGATTTCGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTg                                                                                                                                                                                                                      >  1:561498/1‑251 (MQ=255)                               aaCAAGGCTTCCAGCTCTTCATAACGTTCTTTTAAGCTATCTAATTTATTGATAATAGAGGGTTTCATTATTTGACCTTCAGAAGGAAAATAGAAAAGGAAATAAAAAAATCGGAGCATTCTAGCACAGAATGAAAATGTTGTTGAAGTTTAGGCTGTAAAAAAGGCTTTCGGATTTCGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTAc                                                                                                                                                                                                   <  1:170925/251‑1 (MQ=255)                                     gCTTCCAGCTCTTCATAACGTTCTTTTAAGCTATCTAATTTATTGATAATAGAGGGTTTCATTATTTGACCTTCAGAAGGAAAATAGAAAAGGAAATAAAAAAATCGGAGCATTCTAGCACAGAATGAAAATGTTGTTGAAGTTTAGGCTGTAAAAAAGGCTTTCGGATTTCGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCcgcg                                                                                                                                                                                              <  1:19057/250‑1 (MQ=255)                                                   aTAACGTTCTTTTAAGCTATCTAATTTATTGATAATAGAGGGTTTCATTATTTGACCTTCAGAAGGAAAATAGAAAAGGAAATAAAAAAATCGGAGCATTCTAGCACAGAATGAAAATGTTGTTGAAGTTTAGGCTGTAAAAAAGGCTTTCGGATTTCGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGt                                                                                                                                                                               <  1:425716/251‑1 (MQ=255)                                                         ttCTTTTAAGCTATCTAATTTATTGATAATAGAGGGTTTCATTATTTGACCTTCAGAAGGAAAATAGAAAAGGAAATAAAAAAATCGGAGCATTCTAGCACAGAATGAAAATGTTGTTGAAGTTTAGGCTGTAAAAAAGGCTTTCGGATTTCGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGTTAGGt                                                                                                                                                                          <  1:502983/250‑1 (MQ=255)                                                           cTTTTAAGCTATCTAATTTATTGATAATAGAGGGTTTCATTATTTGACCTTCAGAAGGAAAATAGAAAAGGAAATAAAAAAATCGGAGCATTCTAGCACAGAATGAAAATGTTGTTGAAGTTTAGGCTGTAAAAAAGGCTTTCGGATTTCGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCaaa                                                                                                                                                                                                                          <  1:99818/200‑1 (MQ=255)                                                                     aTCTAATTTATTGATAATAGAGGGTTTCATTATTTGACCTTCAGAAGGAAAATAGAAAAGGAAATAAAAAAATCGGAGCATTCTAGCACAGAATGAAAATGTTGTTGAAGTTTAGGCTGTAAAAAAGGCTTTCGGATTTCGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGTTAGGTTCGGCTTTTTGCa                                                                                                                                                             >  1:496284/1‑251 (MQ=255)                                                                     aTCTAATTTATTGATAATAGAGGGTTTCATTATTTGACCTTCAGAAGGAAAATAGAAAAGGAAATAAAAAAATCGGAGCATTCTAGCACAGAATGAAAATGTTGTTGAAGTTTAGGCTGTAAAAAAGGCTTTCGGATTTCGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGTTAGGTTCGGCTTTTTGCa                                                                                                                                                             >  1:301990/1‑251 (MQ=255)                                                                     aTCTAATTTATTGATAATAGAGGGTTTCATTATTTGACCTTCAGAAGGAAAATAGAAAAGGAAATAAAAAAATCGGAGCATTCTAGCACAGAATGAAAATGTTGTTGAAGTTTAGGCTGTAAAAAAGGCTTTCGGATTTCGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGTTAGGTTCGGCTTTTTGCa                                                                                                                                                             >  1:113142/1‑251 (MQ=255)                                                                         aaTTTATTGATAATAGAGGGTTTCATTATTTGACCTTCAGAAGGAAAATAGAAAAGGAAATAAAAAAATCGGAGCATTCTAGCACAGAATGAAAATGTTGTTGAAGTTTAGGCTGTAAAAAAGGCTTTCGGATTTCGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTaa                                                                                                                                                                                 >  1:295844/1‑227 (MQ=255)                                                                               ttGATAATAGAGGGTTTCATTATTTGACATTCGGAAGGAAAATAGAAAAGGAAATAAAAACATCGGAGCATTCTAGCACAGAATGAAAATGTTGTTGAAGTTTAGGCTGTAAAAAAGGCTTCCGGATTTGGCCAAAAGCCTTTTAGGAATAGGTAG‑TCTTAAAGTGCAGACACGTTCaaa                                                                                                                                                                                                                          >  1:700165/1‑180 (MQ=255)                                                                                   taataGAGGGTTTCATTATTTGACCTTCAGAAGGAAAATAGAAAAGGAAATAAAAAAATCGGAGCATTCTAGCACAGAATGAAAATGTTGTTGAAGTTTAGGCTGTAAAAAAGGCTTTCGGATTTCGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACg                                                                                                                                                                                                                        <  1:587623/178‑1 (MQ=255)                                                                                   taataGAGGGTTTCATTATTTGACCTTCAGAAGGAAAATAGAAAAGGAAATAAAAAAATCGGAGCATTCTAGCACAGAATGAAAATGTTGTTGAAGTTTAGGCTGTAAAAAAGGCTTTCGGATTTCGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGTTAGGTTCGGCTTTTTGCACTACAACAAttt                                                                                                                                                 >  1:847828/1‑249 (MQ=255)                                                                                   taataGAGGGTTTCATTATTTGACCTTCAGAAGGAAAATAGAAAAGGAAATAAAAAAATCGGAGCATTCTAGCACAGAATGAAAATGTTGTTGAAGTTTAGGCTGTAAAAAAGGCTTTCGGATTTCGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACATTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGTTAGGTTCGGCTTTTTGCACTACAACAAttt                                                                                                                                                 >  1:432277/1‑249 (MQ=255)                                                                                           ggTTTCATTATTTGACCTTCAGAAGGAAAATAGAAAAGGAAATAAAAAAATCGGAGCATTCTAGCACAGAATGAAAATGTTGTTGAAGTTTAGGCTGTAAAAAAGGCTTTCGGATTTCGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACg                                                                                                                                                                                                      >  1:863108/1‑188 (MQ=255)                                                                                                  ttattTGACCTTCAGAAGGAAAATAGAAAAGGAAATAAAAAAATCGGAGCATTCTAGCACAGAATGAAAATGTTGTTGAAGTTTAGGCTGTAAAAAAGGCTTTCGGATTTCGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGaaa                                                                                                                                                                                                                                  <  1:77479/153‑1 (MQ=255)                                                                                                                  aGGAAAATAGAAAAGGAAATAAAAAAATCGGAGCATTCTAGCACAGAATGAAAATGTTGTTGAAGTTTAGGCTGTAAAAAAGGCTTTCGGATTTCGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGaaa                                                                                                                                                                                                                                  <  1:285279/137‑1 (MQ=255)                                                                                                                   ggAAAATAGAAAAGGAAATAAAAAAATCGGAGCATTCTAGCACAGAATGAAAATGTTGTTGAAGTTTAGGCTGTAAAAAAGGCTTTCGGATTTCGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGTTAGGTTCGGCTTTTTGCACTACAACAAtt                                                                                                                                                  <  1:869322/216‑1 (MQ=255)                                                                                                                   ggAAAATAGAAAAGGAAATAAAAAAATCGGAGCATTCTAGCACAGAATGAAAATGTTGTTGAAGTTTAGGCTGTAAAAAAGGCTTTCGGATTTCGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGTTAGGTTCGGCTTTTTGCACTACAACAATTTCTTTTCCGTCATCTTTTTTGATTACCATTTCCaa                                                                                                               >  1:383421/1‑251 (MQ=255)                                                                                                                                                     ttCTAGCACAGAATGAAAATGTTGTTGAAGTTTAGGCTGTAAAAAAGGCTTTCGGATTTCGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGaaa                                                                                                                                                                                                                                  >  1:14240/1‑102 (MQ=255)                                                                                                                                                     ttCTAGCACAGAATGAAAATGTTGTTGAAGTTTAGGCTGTAAAAAAGGCTTTCGGATTTCGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGaaa                                                                                                                                                                                                                                  >  1:288734/1‑102 (MQ=255)                                                                                                                                                     ttCTAGCACAGAATGAAAATGTTGTTGAAGTTTAGGCTGTAAAAAAGGCTTTCGGATTTCGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGTTAGGTTCGGCTTTTTGc                                                                                                                                                              <  1:206220/170‑1 (MQ=255)                                                                                                                                                     ttCTAGCACAGAATGAAAATGTTGTTGAAGTTTAGGCTGTAAAAAAGGCTTTCGGATTTCGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGTTAGGTTCGGCTTTTTGCACTACAACAATTTCTTTTCCGTCATCTTTTTTGa                                                                                                                            >  1:84970/1‑204 (MQ=255)                                                                                                                                                     ttCTAGCACAGAATGAAAATGTTGTTGAAGTTTAGGCTGTAAAAAAGGCTTTCGGATTTCGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGTTAGGTTCGGCTTTTTGCACTACAACAATTTCTTTTCCGTCATCTTTTTTGATTACCATTTCCAAGGCGCTAACTTGGCTGACTTTTTCTTCAACCTTa                                                                             >  1:464699/1‑251 (MQ=255)                                                                                                                                                     ttCTAGCACAGAATGAAAATGTTGTTGAAGTTTAGGCTGTAAAAAAGGCTTTCGGATTTCGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGTTAGGTTCGGCTTTTTGCACTACAACAATTTCTTTTCCGTCATCTTTTTTGATTACCATTTCCAAGGCGCTAACTTGGCTGACTTTTTCTTCAACCTTa                                                                             >  1:56985/1‑251 (MQ=255)                                                                                                                                                     ttCTAGCACAGAATGAAAATGTTGTTGAAGTTTAGGCTGTAAAAAAGGCTTTCGGATTTCGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGTTAGGTTCGGCTTTTTGCACTACAACAATTTCTTTTCCGTCATCTTTTTTGATTACCATTTCCAAGGCGCTAACTTGGCTGACTTTTTCTTCAACCTTa                                                                             >  1:657096/1‑251 (MQ=255)                                                                                                                                                     ttCTAGCACAGAATGAAAATGTTGTTGAAGTTTAGGCTGTAAAAAAGGCTTTCGGATTTCGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGTTAGGTTCGGCTTTTTGCACTACAACAATTTCTTTTCCGTCATCTTTTTTGATTACCATTTCCAAGGCGCTAACTTGGCTGACTTTTTCTTCAACCTTa                                                                             >  1:124417/1‑251 (MQ=255)                                                                                                                                                     ttCTAGCACAGAATGAAAATGTTGTTGAAGTTTAGGCTGTAAAAAAGGCTTTCGGATTTCGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGTTAGGTTCGGCTTTTTGCACTACAACAATTTCTTTTCCGTCATCTTTTTTGATTACCATTTCCAAGGCGCTAACTTGGCTGACTTTTTCTTCAACCTTa                                                                             >  1:221217/1‑251 (MQ=255)                                                                                                                                                      tCTAGCACAGAATGAAAATGTTGTTGAAGTTTAGGCTGTAAAAAAGGCTTTCGGATTTCGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGTTAGGTTCGGCTTTTTGCACTACAACAATTTCTTTTCCGTCATCTTTTTTGATTACCATTTCCAAGGCGCTAACTTGGCTGACTTTTTCTTCAACCTTAc                                                                            <  1:307589/251‑1 (MQ=255)                                                                                                                                                        tAGCACAGAATGAAAATGTTGTTGAAGTTTAGGCTGTAAAAAAGGCTTTCGGATTTCGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGTTAGGTTCGGCTTTTTGCACTACAACAATTTCTTTTCCGTCATCTTTTTTGATTACCATTTCCAAGGCGCTAACTTGGCTGACTTTTTCTTCAACCTTACTg                                                                          <  1:694238/251‑1 (MQ=255)                                                                                                                                                               gAATGAAAATGTTGTTGAAGTTTAGGCTGTAAAAAAGGCTTTCGGATTTCGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGtt                                                                                                                                                                              >  1:602022/1‑144 (MQ=255)                                                                                                                                                               gAATGAAAATGTTGTTGAAGTTTAGGCTGTAAAAAAGGCTTTCGGATTTCGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGTTAGGTTCGGCTTTTTGCACTACAACAATTTCTTTTCCGTCATCTTTTTTGATTACCATTTCCAAGGCGCTAACTTGGCTGACTTTTTCTTCAACCTTACTGCCGGTaa                                                                   >  1:105733/1‑251 (MQ=255)                                                                                                                                                                aaTGAAAATGTTGTTGAAGTTTAGGCTGTAAAAAAGGCTTTCGGATTTCGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGTTAGGTTCGGCTTTTTGCACTACAACAATTTCTTTTCCGTCATCTTTTTTGATTACCATTTCCAAGGCGCTAACTTGGCTGACTTTTTCTTCAACCTTACTGCCGGTAAc                                                                  <  1:108684/251‑1 (MQ=255)                                                                                                                                                                       aTGTTGTTGAAGTTTAGGCTGTAAAAAAGGCTTTCGGATTTCGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGTTAGGTTCGGCTTTTTGCACTACAACAATTTCTTTTCCGTCATCTTTTTTGATTACCATTTCCAAGGCGCTAACTTGGCTGACTTTTTCTTCAACCTTACTGCCGGTAACTGCACCg                                                           >  1:84702/1‑251 (MQ=255)                                                                                                                                                                        tgttgtTGAAGTTTAGGCTGTAAAAAAGGCTTTCGGATTTCGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGTTAGGTTCGGCTTTTTGCACTACAACAATTTCTTTTCCGTCATCTTTTTTGATTACCATTTCCAAGGCGCTAACTTGGCTGACTTTTTCTTCAACCTTACTGCCGGTAACTGCAc                                                             <  1:362026/248‑1 (MQ=255)                                                                                                                                                                                 aGTTTAGGCTGTAAAAAAGGCTTTCGGATTTCGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGTTAGGTTCGGCTTTTTGCACTACAACAATTTCTTTTCCGTCATCTTTTTTGATTACCATTTCCAAGGCGCTAACTTGGCTGACTTTTTCTTCAACCTTACTGCCGGTAACTGCACCGGCAATTGCt                                                  >  1:470418/1‑250 (MQ=255)                                                                                                                                                                                  gTTTAGGCTGTAAAAAAGGCTTTCGGATTTCGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGTTAGGTTCGGCTTTTTGCACTACAACAATTTCTTTTCCGTCATCTTTTTTGATTACCATTTCCAAGGCGCTAACTTGGCTGACTTTTTCTTCAACCTTACTGCCGGTAAc                                                                  >  1:306453/1‑233 (MQ=255)                                                                                                                                                                                     tAGGCTGTAAAAAAGGCTTTCGGATTTCGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGTTAGGTTCGGCTTTTTGCACTACAACAATTTCTTTTCCGTCATCTTTTTTGATTACCATTTCCAAGGCGCTAACTTGGCTGACTTTTTCTTCAACCTTACTGCCGGTAACTGCACCGGCAATTGCTCCAAc                                             <  1:571331/251‑1 (MQ=255)                                                                                                                                                                                                           gATTTCGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGTTAGGTTCGGCTTTTTGCACTACAACAATTTCTTTTCCGTCATCTTTTTTGATTACCATTTCCAAGGCGCTAACTTGGCTGACTTTTTCTTCAACCTTACTGCCGGTAACTGCACCGGCAATTGCTCCAACAGCCGTTGCAAGCGCTCTAcc                        <  1:582851/250‑1 (MQ=255)                                                                                                                                                                                                            aTTTCGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGTTAGGTTCGGCTTTTTGCACTACAACAATTTCTTTTCCGTCATCTTTTTTGATTACCATTTCCAAGGCGCTAACTTGGCTGACTTTTTCTTCAACCTTACTGCCGGTAACTGCACCGGCAATTGCTCCAACAGCCGTTGCAAGCGCTCTACCTg                      <  1:573893/251‑1 (MQ=255)                                                                                                                                                                                                                cGCCAAAAGCTTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGTTAGGTTCGGCTTTTTGCACTACAACAATTTCTTCTCCGTCATCTTTTTTGATTACCATTTCCAAGGCGCTAACTTGGCTGACTTTTTCTTCAACCTTACTGCCGGTAACTGCACCGGCAATTGCTCCAACAGCCGTTGCAAGCGCTCTACCTGTacc                  <  1:178385/251‑1 (MQ=255)                                                                                                                                                                                                                cGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGTTAGGTTCGGCTTTTTGCACTACAACAATTTCTTTTCCGTCATCTTTTTTGATTACCATTTCCAAGGCGCTAACTTGGCTGACTTTTTCTTCAACCTTACTGCCGGTAACTGCACCGGCAATTGCTCCAACAGCCGTTGCAAGCGCTCTACCTGTacc                  <  1:573050/251‑1 (MQ=255)                                                                                                                                                                                                                cGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGTTAGGTTCGGCTTTTTGCACTACAACAATTTCTTTTCCGTCATCTTTTTTGATTACCATTTCCAAGGCGCTAACTTGGCTGACTTTTTCTTCAACCTTACTGCCGGTAACTGCACCGGCAATTGCTCCAACAGCCGTTGCAAGCGCTCTACCTGTacc                  <  1:892170/251‑1 (MQ=255)                                                                                                                                                                                                                cGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGTTAGGTTCGGCTTTTTGCACTACAACAATTTCTTTTCCGTCATCTTTTTTGATTACCATTTCCAAGGCGCTAACTTGGCTGACTTTTTCTTCAACCTTACTGCCGGTAACTGCACCGGCAATTGCTCCAACAGCCGTTGCAAGCGCTCTACCTGTacc                  <  1:501794/251‑1 (MQ=255)                                                                                                                                                                                                                cGCCAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGTTAGGTTCGGCTTTTTGCACTACAACAATTTCTTTTCCGTCATCTTTTTTGATTACCATTTCCAAGGCGCTAACTTGGCTGACTTTTTCTTCAACCTTACTGCCGGTAACTGCACCGGCAATTGCTCCAACAGCCGTTGCAAGCGCTCTACCTGTacc                  <  1:303959/251‑1 (MQ=255)                                                                                                                                                                                                                  ccAAAATCCTTTTAGGAATAGGTAGC‑CTTAAAGTGCAGAAACTTTCAACCGTGAACCGCCGACGATACGTACCCGCGCACCTGGCACTAAGTTAGGTTCGGCTTTTTGCACTACAACAATTTCTTTTCCGTCATCTTTTTTGATTACCATTTCCAAGGCGCTAACTTGGCTGACTTTTTCTTCAACCTTACTGCCGGTAACTGCACCGGCAATTGCTCCAACAGCCGTTGCAAGCGCTCTACCTGTaccac                <  1:452355/251‑1 (MQ=255)                                                                                                                                                                                                                  ccAAAAGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGTTAGGTTCGGCTTTTTGCACTACAACAATTTCTTTTCCGTCATCTTTTTTGATTACCATTTCCAAGGCGCTAACTTGGCTGACTTTTTCTTCAACCTTACTGCCGGTAACTGCACCGGCAATTGCTCCAACAGCCGTTGCAAGCGCTCTACCTGTaccac                <  1:531662/251‑1 (MQ=255)                                                                                                                                                                                                                     aaaGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGTTAGGTTCGGCTTTTTGCACTACAACAATTTCTTTTCCGTCATCTTTTTTGATTACCATTTCCAAGGCGCTAACTTGGCTGACTTTTTCTTCAACCTTACTGCCGGTAACTGCACCGGCAATTGCTCCAACAGCCGTTGCAAGCGCTCTACCTGTaccaccac             <  1:385285/251‑1 (MQ=255)                                                                                                                                                                                                                     aaaGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGTTAGGTTCGGCTTTTTGCACTACAACAATTTCTTTTCCGTCATCTTTTTTGATTACCATTTCCAAGGCGCTAACTTGGCTGACTTTTTCTTCAACCTTACTGCCGGTAACTGCACCGGCAATTGCTCCAACAGCCGTTGCAAGCGCTCTACCTGTaccaccac             <  1:55085/251‑1 (MQ=255)                                                                                                                                                                                                                       aGCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGTTAGGTTCGGCTTTTTGCACTACAACAATTTCTTTTCCGTCATCTTTTTTGATTACCATTTCCAAGGCGCTAACTTGGCTGACTTTTTCTTCAACCTTACTGCCGGTAACTGCACCGGCAATTGCTCCAACAGCCGTTGCAAGCGCTCTACCTGTACCACCACCg           >  1:126575/1‑251 (MQ=255)                                                                                                                                                                                                                        gCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGTTAGGTTCGGCTTTTTGCACTACAACAATTTCTTTTCCGTCATCTTTTTTGATTACCATTTCCAAGGCGCTAACTTGGCTGACTTTTTCTTCAACCTTACTGCCGGTAACTGCACCGGCAATTGCTCCAACAGCCGTTGCAAGCGCTCTACCTGTACCACCACCGa          >  1:827675/1‑251 (MQ=255)                                                                                                                                                                                                                        gCCTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGTTAGGTTCGGCTTTTTGCACTACAACAATTTCTTTTCCGTCATCTTTTTTGATTACCATTTCCAAGGCGCTAACTTGGCTGACTTTTTCTTCAACCTTACTGCCGGTAACTGCACCGGCAATTGCTCCAACAGCCGTTGCAAGCGCTCTACCTGTACCACCACCGa          >  1:548659/1‑251 (MQ=255)                                                                                                                                                                                                                         ccTTTTAGGAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGTTAGGTTCGGCTTTTTGCACTACAACAATTTCTTTTCCGTCATCTTTTTTGATTACCATTTCCAAGGCGCTAACTTGGCTGACTTTTTCTTCAACCTTACTGCCGGTAACTGCACCGGCAATTGCTCCAACAGCCGTTGCAAGCGCTCTACCTGTACCACCACCg           <  1:11264/249‑1 (MQ=255)                                                                                                                                                                                                                                 gAATAGGTAG‑ACTTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGTTAGGTTCGGCTTTTTGCACTACAACAATTTCTTTTCCGTCATCTTTTTTGATTACCATTTCCAAGGCGCTAACTTGGCTGACTTTTTCTTCAACCTTACTGCCGGTAACTGCACCGGCAATTGCTCCAACAGCCGTTGCAAGCGCTCTACCTGTACCACCACCGATTGCAGAt  >  1:347780/1‑250 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                              TCCTGATCGCTAATCACGGAAGCATCGCCTAACAAGGCTTCCAGCTCTTCATAACGTTCTTTTAAGCTATCTAATTTATTGATAATAGAGGGTTTCATTATTTGACCTTCAGAAGGAAAATAGAAAAGGAAATAAAAAAATCGGAGCATTCTAGCACAGAATGAAAATGTTGTTGAAGTTTAGGCTGTAAAAAAGGCTTTCGGATTTCGCCAAAAGCCTTTTAGGAATAGGTAGCACCTAAAGTGCAGAAACGTTCAAACGTGAACCGCCGACGATACGTACCCGCGCACCCGGCACTAAGTTAGGTTCGGCTTTTTGCACTACAACAATTTCTTTTCCGTCATCTTTTTTGATTACCATTTCCAAGGCGCTAACTTGGCTGACTTTTTCTTCAACCTTACTGCCGGTAACTGCACCGGCAATTGCTCCAACAGCCGTTGCAAGCGCTCTACCTGTACCACCACCGATTGCAGAT  >  1/1320250‑1320724

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑