Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,319,332	T→C	D339G (GAC→GGC)	unknown ←	Peptide chain release factor 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,319,332	0	T	C	100.0%	205.6 / NA	72	D339G (GAC→GGC)	unknown	Peptide chain release factor 1
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/35);  total (37/35)

GAATAAAAGCGCGAGACTTTTGCGTCACAAATTGCAATAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATCGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGTCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATAAATTACGACGGGTGTCAGCTTGCTCCTGCGCCAGTTTTTCCTGTTCCTGTTGCACCAGACGGGAGGCCAGCACCGATAAAGCTTTGGCTTTGTTTTTATGTTGTGAACGTTCGTCCTGAC  >  1/1319099‑1319578                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                       gAATAAAAGCGCGAGACTTTTGCGTCACAAATTGCAATAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGtt                                                                                                                                                                                                                                        >  1:594684/1‑250 (MQ=255)                 cTTTTGCGTCACAAATTGCAATAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAg                                                                                                                                                                                                                        >  1:717264/1‑250 (MQ=255)                                ttGCAATAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGt                                                                                                                                                                                                        >  1:781187/1‑251 (MQ=255)                                 tGCAATAACACCTGTGCATCGTTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCTTT‑TTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGtt                                                                                                                                                                                                       <  1:236512/251‑1 (MQ=255)                                  gCAATACCCCCTGTGCATCGGTTTTGCCTTTTTCCGGGGGTTTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAgg                                                                                                                                                                                                         <  1:347198/248‑1 (MQ=255)                                  gCAATAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTa                                                                                                                                                                                                      >  1:345263/1‑251 (MQ=255)                                   cAATAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTaa                                                                                                                                                                                                                  >  1:129850/1‑238 (MQ=255)                                   cAATAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTaa                                                                                                                                                                                                     >  1:789100/1‑251 (MQ=255)                                      taccacctttccttcgttgtttccATTTTCCGATGTTTTCCCCTGATTAATCGTTTGTATGGCTTTTTCCAGCTTTT‑TTTGGTATTTCATTTTAAAAAAATGACCGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATAc                                                                                                                                                                                                  <  1:799127/229‑1 (MQ=255)                                      tAACCCCTGTGCATCGGTTTTGCCATTTCCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATAc                                                                                                                                                                                                  <  1:80783/251‑1 (MQ=255)                                        acacCGGTGCTTCGGTTTTGCCTTTTTCCGAGGGTTTCGCCTGATTTAACGTTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACgg                                                                                                                                                                                                <  1:459667/251‑1 (MQ=255)                                           ccTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGa                                                                                                                                                                                             >  1:276307/1‑251 (MQ=255)                                              gtgCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCg                                                                                                                                                                                          >  1:473692/1‑251 (MQ=255)                                                    cgtttttGCCTTTTTCCGAGGGATTCGCTTGATTTAACGCTTGTATGGCCTGTTGCAGCCTTT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAAcg                                                                                                                                                                                    <  1:759391/248‑1 (MQ=255)                                                      gTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTATTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACgg                                                                                                                                                                                                <  1:205809/237‑1 (MQ=255)                                                                tttCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCggg                                                                                                                                                                        >  1:463539/1‑251 (MQ=255)                                                                            tCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTCCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTg                                                                                                                                                            >  1:841098/1‑251 (MQ=255)                                                                                  gATTTAACGTTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTg                                                                                                                                                                  <  1:742833/239‑1 (MQ=255)                                                                                  gATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGAtt                                                                                                                                                      >  1:734095/1‑251 (MQ=255)                                                                                         cGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCgg                                                                                                                                               >  1:156095/1‑251 (MQ=255)                                                                                          tcTTGTATGGCGTGGTGCA‑CCGTTGGTTGGTTGTTGATTGTAAAAAAAGGATCGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTGAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCgg                                                                                                                                               <  1:47754/249‑1 (MQ=255)                                                                                           cTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCATCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGaa                                                                                                                                             >  1:426457/1‑251 (MQ=255)                                                                                             tGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTTTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACgg                                                                                                                                                                                                <  1:509750/198‑1 (MQ=255)                                                                                             tGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGt                                                                                                                                                                       >  1:854003/1‑223 (MQ=255)                                                                                             tGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACg                                                                                                                                           >  1:80700/1‑251 (MQ=255)                                                                                             tGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACg                                                                                                                                           >  1:318024/1‑251 (MQ=255)                                                                                              gTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGa                                                                                                                                          >  1:159766/1‑251 (MQ=255)                                                                                                   gCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGa                                                                                                                                                                                             >  1:889776/1‑195 (MQ=255)                                                                                                   gCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTg                                                                                                                                                                            >  1:500349/1‑212 (MQ=255)                                                                                                   gCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGc                                                                                                                                      >  1:704459/1‑250 (MQ=255)                                                                                                               cATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTg                                                                                                                                                                            >  1:63555/1‑200 (MQ=255)                                                                                                                ttt‑gttgGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATaaa                                                                                                                        <  1:613902/250‑1 (MQ=255)                                                                                                                    gttgGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATAAATTa                                                                                                                     >  1:350483/1‑251 (MQ=255)                                                                                                                    gttgGTAGTTCATTGTAAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACgg                                                                                                                                                                                                <  1:338220/176‑1 (MQ=255)                                                                                                                     ttgttatttcatgtttAAAAAAGGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATAAATTac                                                                                                                    <  1:760181/237‑1 (MQ=255)                                                                                                                        gtatttCATTGTAAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATAAATTacga                                                                                                                  <  1:630537/246‑1 (MQ=255)                                                                                                                           gttcatttttAAAAAAGGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATAAATTacgacg                                                                                                                <  1:834960/241‑1 (MQ=255)                                                                                                                                   ttAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATAAATTACGACGGGTGTCAGc                                                                                                       <  1:789504/250‑1 (MQ=255)                                                                                                                                               cGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATAAATTACGACGGGTGTCAGCTTGCTCCTGCGcc                                                                                          <  1:257191/251‑1 (MQ=255)                                                                                                                                                  gCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATAAATTACGACGGGTGTCAGCTTGCTCCTGCGCCAGt                                                                                       >  1:525526/1‑251 (MQ=255)                                                                                                                                                   ccGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATAAATTACGACGGGTGTCAGCTTGCTCCTGCGCCAGtt                                                                                      >  1:35041/1‑251 (MQ=255)                                                                                                                                                               ttCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATAAATTACGACGGGTGTCAGCTTGCTCCTGCGCCAGTTTttcctgttcct                                                                          <  1:6946/251‑1 (MQ=255)                                                                                                                                                                 ctcATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATAAATTACGACGGGTGTCAGCTTGCTcc                                                                                                >  1:849836/1‑227 (MQ=255)                                                                                                                                                                 ctcATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATAAATTACGACGGGTGTCAGCTTGCTCCTGCGCCAGTTTttcctgttc                                                                            >  1:710766/1‑247 (MQ=255)                                                                                                                                                                      ggTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACg                                                                                                                                           <  1:629093/179‑1 (MQ=255)                                                                                                                                                                      ggTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATAAATTACGACGGGTGTCAGCTTGCTCCTGCGCCAGTTTTTCCTGTTCCTGTTGCa                                                                    >  1:53613/1‑250 (MQ=255)                                                                                                                                                                      ggTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATAAATTACGACGGGTGTCAGCTTGCTCCTGCGCCAGTTTTTCCTGTTCCTGTTGCa                                                                    >  1:768897/1‑250 (MQ=255)                                                                                                                                                                       gTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATAAATTACGACGGGTGTCAGCTTGCTCCTGCGCCAGTTTTTCCTGTTCCTGTTGCAc                                                                   <  1:514941/250‑1 (MQ=255)                                                                                                                                                                       gTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATAAATTACGACGGGTGTCAGCTTGCTCCTGCGCCAGTTTTTCCTGTTCCTGTTGCAc                                                                   >  1:788055/1‑250 (MQ=255)                                                                                                                                                                           aTTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATAAATTACGACGGGTGTCAGCTTGCTCCTGCGCCAGTTTTTCCTGTTCCTGTTGCACCAGa                                                               >  1:733016/1‑250 (MQ=255)                                                                                                                                                                           aTTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATAAATTACGACGGGTGTCAGCTTGCTCCTGCGCCAGTTTTTCCTGTTCCTGTTGCACCAGAc                                                              <  1:736818/251‑1 (MQ=255)                                                                                                                                                                                gCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATAAATTACGACGGGTGTCAGCTTGCTCCTGCGCCAGTTTTTcctgttcctgtt                                                                       <  1:108065/237‑1 (MQ=255)                                                                                                                                                                                        aaCGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATAAATTACGACGGGTGTCAGCTTGCTCCTGCGCCAGTTTTTCCTGTTCCTGTTGCACCAGACGGGAGGCCAGCAc                                                 <  1:728818/251‑1 (MQ=255)                                                                                                                                                                                        aaCGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATAAATTACGACGGGTGTCAGCTTGCTCCTGCGCCAGTTTTTCCTGTTCCTGTTGCACCAGACGGGAGGCCAGCAc                                                 <  1:70721/251‑1 (MQ=255)                                                                                                                                                                                        aaCGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATAAATTACGACGGGTGTCAGCTTGCTCCTGCGCCAGTTTTTCCTGTTCCTGTTGCACCAGACGGGAGGCCAGCAc                                                 <  1:258941/251‑1 (MQ=255)                                                                                                                                                                                                cAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATAAATTACGACGGGTGTCAGCTTGCTcc                                                                                                >  1:78082/1‑196 (MQ=255)                                                                                                                                                                                                cAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATAAATTACGACGGGTGTCAGCTTGCTCCTgcg                                                                                            <  1:886376/200‑1 (MQ=255)                                                                                                                                                                                                 aaCTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATAAATTACGACGGGTGTCAGCTTGCTCCTGCGCCAGTTTTTCCTGTTCCTGTTGCACCAGACGGGAGGCCAGCACCGATAAAgc                                        <  1:887659/251‑1 (MQ=255)                                                                                                                                                                                                 aaCTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATAAATTACGACGGGTGTCAGCTTGCTCCTGCGCCAGTTTTTCCTGTTCCTGTTGCACCAGACGGGAGGCCAGCACCGATAAAgc                                        <  1:749322/251‑1 (MQ=255)                                                                                                                                                                                                       tCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATAAATTACGACGGGTGTCAGCTTGCTCCTGCGCCAGTTTTTCCTGTTCCTGTTGCACCAGACGGGAGGCCAGCACCGATAAAgctttggc                                  <  1:101383/251‑1 (MQ=255)                                                                                                                                                                                                       tCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATAAATTACGACGGGTGTCAGCTTGCTCCTGCGCCAGTTTTTCCTGTTCCTGTTGCACCAGACGGGAGGCCAGCACCGATAAAgctttggc                                  <  1:550984/251‑1 (MQ=255)                                                                                                                                                                                                        ccGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATAAATTACGACGGGTGTCAGCTTGCTCCTGCGCCAGTTTTTCCTGTTCCTGTTGCACCAGACGGGAGGCCAGCACCGATAAAgctttggct                                 <  1:362601/251‑1 (MQ=255)                                                                                                                                                                                                                   tCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATAAATTACGACGGGTGTCAGCTTGCTCCTGCGCCAGTTTTTCCTGTTCCTGTTGCACCAGACGGGAGGCCAGCACCGATAAAGCTTTGGCTTTGTTTTTAtg                      <  1:750928/251‑1 (MQ=255)                                                                                                                                                                                                                      gTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATAAATTACGACGGGTGTCAGCTTGCTCCTGCGCCAGTTTTTCCTGTTCCTGTTGCACCAGACGGGAGGCCAGCACCGATAAAGCTTTGGCTTTGTTTTTAtgttg                   >  1:823764/1‑251 (MQ=255)                                                                                                                                                                                                                      gTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATAAATTACGACGGGTGTCAGCTTACTCCTGCGCCAGTTTTTCCTGTTCCTGTTGCACCAGACGGGAGGCCAGCACCGATAAAGCTTTGGCTTTGTTTTTAtgttg                   >  1:446177/1‑251 (MQ=255)                                                                                                                                                                                                                              cGGTTGAATCAAGCCATCAATTTTGTCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATAAATTACGACGGGTGTCAGCTTGCTCCTGCGCCAGTTTTTCCTGTTCCTGTTGCACCAGACGGGAGGCCAGCACCGATAAAGCTTTGGCTTTGTTTTTATGTTGTGAACGtt           <  1:53531/251‑1 (MQ=255)                                                                                                                                                                                                                              cGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATAAATTACGACGGGTGTCAGCTTGCTCCTGCGCCAGTTTTTCCTGTTCCTGTTGCACCAGACGGGAGGCCAGCACCGATAAAGCTTTGGCTTTGTTTTTATGTTGTGAACGtt           >  1:412074/1‑251 (MQ=255)                                                                                                                                                                                                                               ggTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGACTTTATCGGAACGATCGCCGGAACCCAATAAATTACGACGGGTGTCAGCTTGCTCCTGCGCCAGTTTTTCCTGTTCCTGTTGCACCAGACGGGAGGCCAGCACCGATAAAGCTTTGGCTTTGTTTTTATGTTGTGAACGtt           >  1:888880/1‑250 (MQ=255)                                                                                                                                                                                                                                gTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGt                                                                                                                                                                       <  1:509066/93‑1 (MQ=255)                                                                                                                                                                                                                                gTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATAAATTACGACGGGTGTCAGCTTGCTCCTGCGCCAGTTTTTCCTGTTCCTGTTGCACCAGACGGGAGGCCAGCACCGATAAAGCTTTGGCTTTGTTTTTATGTTGTGAACGTTCg         >  1:865421/1‑251 (MQ=255)                                                                                                                                                                                                                                   gAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATAAATTACGACGGGTGTCAGCTTGCTCCTGCGCCAGTTTTTCCTGTTCCTGTTGCACCAGACGGGAGGCCAGCACCGATAAAGCTTTGGCTTTGTTTTTATGTTGTGAACGTTCGTc       >  1:357398/1‑250 (MQ=255)                                                                                                                                                                                                                                       cAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATAAATTACGACGGGTGTCAGCTTGCTCCTGCGCCAGTTTTTCCTGTTCCTGTTGCACCAGACGGGAGGCCAGCACCGATAAAGCTTTGGCTTTGTTTTTATGTTGTGAACGTTCGTCCTGAc  <  1:386748/251‑1 (MQ=255)                                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                       GAATAAAAGCGCGAGACTTTTGCGTCACAAATTGCAATAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATCGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGTCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATAAATTACGACGGGTGTCAGCTTGCTCCTGCGCCAGTTTTTCCTGTTCCTGTTGCACCAGACGGGAGGCCAGCACCGATAAAGCTTTGGCTTTGTTTTTATGTTGTGAACGTTCGTCCTGAC  >  1/1319099‑1319578

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑