Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,319,226	C→T	intergenic (‑1/+39)	unknown ← / ← unknown	Peptide chain release factor N(5)‑glutamine methyltransferase (EC 2.1.1.297)/Peptide chain release factor 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,319,226	0	C	T	100.0%	199.6 / NA	75	intergenic (‑1/+39)	unknown/unknown	Peptide chain release factor N(5)‑glutamine methyltransferase (EC 2.1.1.297)/Peptide chain release factor 1
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (44/31);  total (44/31)

ACCAAAACTCTTTTTCACCGAGAATATAAGCGATGGGTTCGCCCTGCAAACGGCGGGCTAAAAGTGCGGTCAGTTTTTCCAGCGTTTTTTCATCCAATGCCGTTTCACCGAAAGCCAGAATAAAAGCGCGAGACTTTTGCGTCACAAATTGCAATAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATCGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGTCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATAAATTACGA  >  1/1318982‑1319466                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 aCCAAAACTCTTTTTCACCGAGAATATAAGCGATGGGTTCGCCCTGCAAACGGCAGGCTAAAAGTGCGGTCAGTTTTTCCAGCGTTTTTTCATCCAATGCCGTTTCACCGAAAGCCAGAATAAAAGCGCGAGACTTTTGCGTCACAAATTGCAATAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTaaa                                                                                                                                                                                                                                            >  1:645523/1‑251 (MQ=255)    atgtgtataaagagacaggaATATAAGCGATGGGTTCGCCCTGCAAACGGCAGGCTAAAAGTGCGGTCAGTTTTTCCAGCGTTTTTTCATCCAATGCCGTTTCACCGAAAGCCAGAATAAAAGCGCGAGACTTTTGCGTCACAAATTGCAATAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTaaaaaa                                                                                                                                                                                                                                         <  1:533333/233‑1 (MQ=255)            ttttCACCGAGAATATAAGCGATGGGTTCGCCCTGCAAACGGCAGGCTAAAAGTGCGGTCAGTTTTTCCAGCGTTTTTTCATCCAATGCCGTTTCACCGAAAGCCAGAATAAAAGCGCGAGACTTTTGCGTCACAAATTGCAATAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAg                                                                                                                                                                                                                                 >  1:582942/1‑251 (MQ=255)                    gagaATATAAGCGATGGGTTCGCCCTGCAAACGGCAGGCTAAAAGTGCGGTCAGTTTTTCCAGCGTTTTTTCATCCAATGCCGTTTCACCGAAAGCCAGAATAAAAGCGCGAGACTTTTGCGTCACAAATTGCAATAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAg                                                                                                                                                                                                                         >  1:591737/1‑251 (MQ=255)                      gaATATAAGCGATGGGTTCGCCCTGCAAACGGCAGGCTAAAAGTGCGGTCAGTTTTTCCAGCGTTTTTTCATCCAATGCCGTTTCACCGAAAGCCAGAATAAAAGCGCGAGACTTTTGCGTCACAAATTGCAATAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTaaaa                                                                                                                                                                                                                                           >  1:57471/1‑231 (MQ=255)                      gaATATAAGCGATGGGTTCGCCCTGCAAACGGCAGGCTAAAAGTGCGGTCAGTTTTTCCAGCGTTTTTTCATCCAATGCCGTTTCACCGAAAGCCAGAATAAAAGCGCGAGACTTTTGCGTCACAAATTGCAATAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTaaaa                                                                                                                                                                                                                                           <  1:233139/231‑1 (MQ=255)                      gaATATAAGCGATGGGTTCGCCCTGCAAACGGCAGGCTAAAAGTGCGGTCAGTTTTTCCAGCGTTTTTTCATCCAATGCCGTTTCACCGAAAGCCAGAATAAAAGCGCGAGACTTTTGCGTCACAAATTGCAATAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAg                                                                                                                                                                                                                         >  1:561415/1‑249 (MQ=255)                      gaATATAAGCGATGGGTTCGCCCTGCAAACGGCAGGCTAAAAGTGCGGTCAGTTTTTCCAGCGTTTTTTCATCCAATGCCGTTTCACCGAAAGCCAGAATAAAAGCGCGAGACTTTTGCGTCACAAATTGCAATAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAg                                                                                                                                                                                                                         >  1:758951/1‑249 (MQ=255)                      gaATATAAGCGATGGGTTCGCCCTGCAAACGGCAGGCTAAAAGTGCGGTCAGTTTTTCCAGCGTTTTTTCATCCAATGCCGTTTCACCGAAAGCCAGAATAAAAGCGCGAGACTTTTGCGTCACAAATTGCAATAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAg                                                                                                                                                                                                                         >  1:276145/1‑249 (MQ=255)                       aaTATAAGCGATGGGTTCGCCCTGCAAACGGCAGGCTAAAAGTGCGGTCAGTTTTTCCAGCGTTTTTTCATCCAATGCCGTTTCACCGAAAGCC‑GAATAAAAGCGCGAGACTTTTGCGTCAAAAATTGCAATAACCCCTGTGCATCGGTTTTGCCATTTTCCGAGGGTTTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCt                                                                                                                                                                                                                       <  1:237154/249‑1 (MQ=255)                           aaaGCGAGGGGTTCGCCCTGCAAACGGCAGGCTAAAAGTGCGGTCAGTTTTTCCAGCGTTTTTTCATCCAATGCCGTTTCACCGAAAGCCAGAATAAAAGCGCGAGACTTTTGCGTCACAAATTGCAATAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTtc                                                                                                                                                                                                                  <  1:701005/250‑1 (MQ=255)                             aGCGATGGGTTCGCCCTGCAAACGGCAGGCTAAAAGTGCGGTCAGTTTTTCCAGCGTTTTTTCATCCAATGCCGTTTCACCGAAAGCCAGAATAAAAGCGCGAGACTTTTGCGTCACAAATTGCAATAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGc                                                                                                                                                                                                                        >  1:836905/1‑243 (MQ=255)                              gCGATGGTTTCGCCCTGCAAACGGCAGGCTAAAAGTGCGGTCAGTTTTTCCAGCTTTTTTTCATCCAATGCCGTTTCACCGAAAGCCAGAATAAAAGCGCGAGATTTTTGCGTCCCAAATGGCAATAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTATTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTtctc                                                                                                                                                                                                                <  1:469393/250‑1 (MQ=255)                                 aTGGGTTCGCCCTGCAAACGGCAGGCTAAAAGTGCGGTCAGTTTTTCCAGCGTTTTTTCATCCAATGCCGTTTCACCGAAAGCCAGAATAAAAGCGCGAGGCTTTTGCGTCACAAATTGAAATAACACCTGTGCATGGGTTTTGCCTTTTTCCGAGGGATTCGCCTGATTTAACGTTTGTATGGCCTGTTGCAGCCATT‑TTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATgg                                                                                                                                                                                                            <  1:82081/251‑1 (MQ=255)                                    gtttCGCCCTGCAAACGGCAGGCTAAAAGTGCGGTCAGTTTTTCCAGCGTTTTTTCATCCAATGCCGTTTCACCGAAAGCCAGAATAAAAGCGCGAGACTTTTGCGTCACAAATTGCAATAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTTTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGtt                                                                                                                                                                                                          <  1:792246/248‑1 (MQ=255)                                        cGCCCTGCAAACGGCAGGCTAAAAGTGCGGTCAGTTTTTCCAGCGTTTTTTCATCAAATGCCGTTTCACCGAAAGCCAGAATAAAAGCGCGAGTCTTTTGCGTCAAAATTTGCAATAACCCCTGTGCATCGTTTTTGCCATTTTCCGAGGGATTCGCCTGATTAAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTAAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTc                                                                                                                                                                                                     <  1:143876/251‑1 (MQ=255)                                                   cggcagGCTAAAAGTGCGGTCAGTTTTTCCAGCGTTTTTTCATCCAATGCCGTTTCACCGAAAGCCAGAATAAAAGCGCGAGACTTTTGCGTCACAAATTGCAATAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACaa                                                                                                                                                                                          >  1:278483/1‑251 (MQ=255)                                                        ggCTAAAAGTGCGGTCAGTTTTTCCAGCGTTTTTTCATCCAATGCCGTTTCACCGAAAGCCAGAATAAAAGCGCGAGACTTTTGCGTCACAAATTGCAATAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTtc                                                                                                                                                                                                                  >  1:740558/1‑222 (MQ=255)                                                        ggCTAAAAGTGCGGTCAGTTTTTCCAGCGTTTTTTCATCCAATGCCGTTTCACCGAAAGCCAGAATAAAAGCGCGAGACTTTTGCGTCACAAATTGCAATAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAAcgccg                                                                                                                                                                                     >  1:491327/1‑251 (MQ=255)                                                             aaaGTGCGGTCAGTTTTTCCAGCGTTTTTTCATCCAATGCCGTTTCACCGAAATCCAGAATAAAAGCGCGAGACTTTTGCGTCACAAATTGCAATAACCCCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCTCCTGATTTAGCGCTTGTATGTCCTGTTGCAGCCATG‑GTTGGTTATTCATTGAAAAAAAAGGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAAc                                                                                                                                                                                <  1:286944/251‑1 (MQ=255)                                                                      tcattttttCCAGCTTTTTTTCATCCAATCCCTTTTCACCAAAAGCCAGAATAAAAGCGCGAGACTTTTGCGTCACAAATTGCAATAACCCCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGCCCTGTTGCAGCCATT‑GTTGGTATTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGc                                                                                                                                                                        <  1:190334/246‑1 (MQ=255)                                                                       caaaattcgcAGCGTTTTTTCATCCAATGCCGTTTCACCGAAAGCCAGAATAAAAGCGCGAGACTTTTGCGTCAAAAATTGCAATAACCCCTGTGCATCGTTTTTGCCATTTCCCGAGGGATTCGCCTGATTTACCGCTTGTATGCCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGc                                                                                                                                                                                                                        <  1:535897/192‑1 (MQ=255)                                                                            tttCCAGCGTTTTTTCATCCAATGCCGTTTCACCGAAAGCCAGAATAAAAGCGCGAGACTTTTGCGTCACAAATTGCAATAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGatat                                                                                                                                                                 >  1:583297/1‑251 (MQ=255)                                                                            tttCCAGCGTTTTTTCATCCAATGCCGTTTCACCGAAAGCCAGAATAAAAGCGCGAGACTTTTGCGTCACAAATTGAAATAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGatat                                                                                                                                                                 <  1:207866/251‑1 (MQ=255)                                                                                      tttttCATCCAATGCCGTTTCACCGAAAGCCAGAATAAAAGCGCGAGACTTTTGCGTCACAAATTGCAATAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGcc                                                                                                                                                                                                                               <  1:517502/179‑1 (MQ=255)                                                                                       ttttCATCCAATGCCGTTTCACCGAAAGCCAGAATAAAAGCGCGAGACTTTTGCGTCACAAATTGCAATAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAAt                                                                                                                                                      >  1:662686/1‑251 (MQ=255)                                                                                       ttttCATCCAATGCCGTTTCACCGAAAGCCAGAATAAAAGCGCGAGACTTTTGCGTCACAAATTGCAATAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTCAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAAt                                                                                                                                                      >  1:295794/1‑251 (MQ=255)                                                                                            aTCCAATGCCGTTTCACCGAAAGCCAGAATAAAAGCGCGAGACTTTTGCGTCACAAATTGCAATAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGtt                                                                                                                                                 >  1:516065/1‑251 (MQ=255)                                                                                            aTCCAATGCCGTTTCACCGAAAGCCAGAATAAAAGCGCGAGACTTTTGCGTCACAAATTGCAATAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGtt                                                                                                                                                 >  1:274340/1‑251 (MQ=255)                                                                                                aaTGCCGTTTCCCCGAAAGCCAGAATAAAAGCGCGAGACTTTTGCGTCACAAATTGCAATAACCCCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAAt                                                                                                                                             <  1:665458/251‑1 (MQ=255)                                                                                                  tGCCGTTTCACCGAAAGCCAGAATAAAAGCGCGAGACTTTTGCGTCACAAATTGCAATAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTaaaaaa                                                                                                                                                                                                                                         >  1:139276/1‑157 (MQ=255)                                                                                                     cGTTTCACCGAAAGCCAGAATAAAAGCGCGAGACTTTTGCGTCACAAATTGCAATAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAACTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAg                                                                                                                                         >  1:425203/1‑250 (MQ=255)                                                                                                         tccccGAAAGCCAGAATAAAAGCGCGAGCCTTTTGCGTCACAAATTGCAATAACCCCTGTGCATCGTTTTTGCCTTTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCTTT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGt                                                                                                                                                            <  1:276268/224‑1 (MQ=255)                                                                                                                      gAATAAAAGCGCGAGACTTTTGCGTCACAAATTGCAATAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGtt                                                                                                                        >  1:594684/1‑250 (MQ=255)                                                                                                                                      cTTTTGCGTCACAAATTGCAATAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAg                                                                                                        >  1:717264/1‑250 (MQ=255)                                                                                                                                                     ttGCAATAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGt                                                                                        >  1:781187/1‑251 (MQ=255)                                                                                                                                                      tGCAATAACACCTGTGCATCGTTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCTTT‑TTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGtt                                                                                       <  1:236512/251‑1 (MQ=255)                                                                                                                                                       gCAATACCCCCTGTGCATCGGTTTTGCCTTTTTCCGGGGGTTTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAgg                                                                                         <  1:347198/248‑1 (MQ=255)                                                                                                                                                       gCAATAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATgg                                                                                                                                                                                                            >  1:55006/1‑133 (MQ=255)                                                                                                                                                       gCAATAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTa                                                                                      >  1:345263/1‑251 (MQ=255)                                                                                                                                                        cAATAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTaa                                                                                                  >  1:129850/1‑238 (MQ=255)                                                                                                                                                        cAATAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTaa                                                                                     >  1:789100/1‑251 (MQ=255)                                                                                                                                                         aaTAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATgg                                                                                                                                                                                                            >  1:27468/1‑131 (MQ=255)                                                                                                                                                           taccacctttccttcgttgtttccATTTTCCGATGTTTTCCCCTGATTAATCGTTTGTATGGCTTTTTCCAGCTTTT‑TTTGGTATTTCATTTTAAAAAAATGACCGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATAc                                                                                  <  1:799127/229‑1 (MQ=255)                                                                                                                                                           tAACCCCTGTGCATCGGTTTTGCCATTTCCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATAc                                                                                  <  1:80783/251‑1 (MQ=255)                                                                                                                                                             acacCGGTGCTTCGGTTTTGCCTTTTTCCGAGGGTTTCGCCTGATTTAACGTTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACgg                                                                                <  1:459667/251‑1 (MQ=255)                                                                                                                                                              cacCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGtt                                                                                                                                                                                                          <  1:670434/128‑1 (MQ=255)                                                                                                                                                              cacCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCAATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATg                                                                                                                                                                                                             <  1:165705/125‑1 (MQ=255)                                                                                                                                                                ccTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGatat                                                                                                                                                                 >  1:484186/1‑167 (MQ=255)                                                                                                                                                                ccTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGa                                                                             >  1:276307/1‑251 (MQ=255)                                                                                                                                                                   gtgCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCg                                                                          >  1:473692/1‑251 (MQ=255)                                                                                                                                                                         cgtttttGCCTTTTTCCGAGGGATTCGCTTGATTTAACGCTTGTATGGCCTGTTGCAGCCTTT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAAcg                                                                    <  1:759391/248‑1 (MQ=255)                                                                                                                                                                           gTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTATTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACgg                                                                                <  1:205809/237‑1 (MQ=255)                                                                                                                                                                           gTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATc                                                                                                                                                                           >  1:67387/1‑146 (MQ=255)                                                                                                                                                                                     tttCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCggg                                                        >  1:463539/1‑251 (MQ=255)                                                                                                                                                                                                 tCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTCCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTg                                            >  1:841098/1‑251 (MQ=255)                                                                                                                                                                                                       gATTTAACGTTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTg                                                  <  1:742833/239‑1 (MQ=255)                                                                                                                                                                                                       gATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGAtt                                      >  1:734095/1‑251 (MQ=255)                                                                                                                                                                                                              cGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCgg                               >  1:156095/1‑251 (MQ=255)                                                                                                                                                                                                               tcTTGTATGGCGTGGTGCA‑CCGTTGGTTGGTTGTTGATTGTAAAAAAAGGATCGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTGAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCgg                               <  1:47754/249‑1 (MQ=255)                                                                                                                                                                                                                cTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCATCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGaa                             >  1:426457/1‑251 (MQ=255)                                                                                                                                                                                                                  tGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTTTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACgg                                                                                <  1:509750/198‑1 (MQ=255)                                                                                                                                                                                                                  tGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGt                                                       >  1:854003/1‑223 (MQ=255)                                                                                                                                                                                                                  tGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACg                           >  1:80700/1‑251 (MQ=255)                                                                                                                                                                                                                  tGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACg                           >  1:318024/1‑251 (MQ=255)                                                                                                                                                                                                                   gTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGa                          >  1:159766/1‑251 (MQ=255)                                                                                                                                                                                                                        gCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGa                                                                             >  1:889776/1‑195 (MQ=255)                                                                                                                                                                                                                        gCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTg                                                            >  1:500349/1‑212 (MQ=255)                                                                                                                                                                                                                        gCCTGTTGCAGCCATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGc                      >  1:704459/1‑250 (MQ=255)                                                                                                                                                                                                                                    cATT‑GTTGGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTg                                                            >  1:63555/1‑200 (MQ=255)                                                                                                                                                                                                                                     ttt‑gttgGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATaaa        <  1:613902/250‑1 (MQ=255)                                                                                                                                                                                                                                         gttgGTAGTTCATTGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATAAATTa     >  1:350483/1‑251 (MQ=255)                                                                                                                                                                                                                                         gttgGTAGTTCATTGTAAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACgg                                                                                <  1:338220/176‑1 (MQ=255)                                                                                                                                                                                                                                             gtatttCATTGTAAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGCCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATAAATTacga  <  1:630537/246‑1 (MQ=255)                                                                                                                                                                                                                                             gTAGTTCATTGTAAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATc                                                                                                                                                     <  1:247997/103‑1 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 ACCAAAACTCTTTTTCACCGAGAATATAAGCGATGGGTTCGCCCTGCAAACGGCGGGCTAAAAGTGCGGTCAGTTTTTCCAGCGTTTTTTCATCCAATGCCGTTTCACCGAAAGCCAGAATAAAAGCGCGAGACTTTTGCGTCACAAATTGCAATAACACCTGTGCATCGGTTTTGCCATTTTCCGAGGGATTCGCCTGATTTAACGCTTGTATGGCCTGTTGCAGCCATT‑GTTGGTAGTTCATCGTTAAAAAATGAACGAGCCGTTAAGCTCGTTCTCATGGTTAATTCTGCTCCGACAACGCCGCCAACTGATCCGCTTGATATTCGGTGATAATCGGTTGAATCAAGTCATCAATTTTGCCGTTCATCACTTCGTCTAAGCGGTAAATCGTCAGGTTAATACGGTGATCGGTAACGCGCCCTTGCGGGTAATTGTAAGTGCGGATTTTATCGGAACGATCGCCGGAACCCAATAAATTACGA  >  1/1318982‑1319466

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑