Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,316,827	A→G	S152S (TCT→TCC)	unknown ←	Cell wall‑associated hydrolases (invasion‑associated proteins)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,316,827	0	A	G	100.0%	186.0 / NA	65	S152S (TCT→TCC)	unknown	Cell wall‑associated hydrolases (invasion‑associated proteins)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (25/40);  total (25/40)

TCTGGTAATCAGATTGCACGTGAGTAAGTTTGACGCAATATGACCGTTTTTTTCCGCTTGCTTACGTCGGCGGATGATATGATGTTTGAATTCGATTGAATAATGTTTGACCATAAAAATCCATAGTTTAGTTGGTTGTGGTATCTGATTGGGATGCAGAATACTTTTTATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAAGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGCCTACATGATTATTTCTACGGAAAAAAACCAAATCGCCCACTTTCAGTTCTTTCTTTTGAATCGCTTTACCGACATAACGCTGTTCGGAAGTCGAACGCGGCAATGTCACACCAAACGCATCTAAAAAGGTGGTTTGCATAAAGCCGGAACAATCAATGCCATTTTTTGTGGTGCCGCCAAA  >  1/1316587‑1317072                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      tCTGGTAATCAGATTGCACGTGAGTAAGTTTGACGCAATATGACCGTTTTTTTCCGCTTGCTTACGTCGGCGGATGATATGATGTTTGAATTCGATTGAATAATGTTTGACCATAAAAATCCATAGTTTAGTTGGTTGTGGTATCTGATTGGGATGCAGAATACTTTTTATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCaataa                                                                                                                                                                                                                                             >  1:337925/1‑251 (MQ=255)     gTAATCAGATTGCACGTGAGTAAGTTTGACGCAATATGACCGTTTTTTTCCGCTTGCTTACGTCGGCGGATGATATGATGTTTGAATTCGATTGAATAATGTTTGACCATAAAAATCCATAGTTTAGTTGGTTGTGGTATCTGATTGGGATGCAGAATACTTTTTATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCa                                                                                                                                                                                                                                          <  1:843269/250‑1 (MQ=255)             aTTGCACGTGAGTAAGTTTGACGCAATATGACCGTTTTTTTCCGCTTGCTTACGTCGGCGGATGATATGATGTTTGAATTCGATTGAATAATGTTTGACCATAAAAATCCATAGTTTAGTTGGTTGTGGTATCTGATTGGGATGCAGAATACTTTTTATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGc                                                                                                                                                                                                                                 <  1:274980/251‑1 (MQ=255)              ttGCACGTGAGTAAGTTTGACGCAATATGACCGTTTTTTTCCGCTTGCTTACGTCGGCGGATGATATGATGTTTGAATTCGATTGAATAATGTTTGACCATAAAAATCCATAGTTTAGTTGGTTGTGGTATCTGATTGGGATGCAGAATACTTTTTATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCgg                                                                                                                                                                                                                                  >  1:777167/1‑249 (MQ=255)                    gTGAGTAAGTTTGACGCAATATGACCGTTTTTTTCCGCTTGCTTACGTCGGCGGATGATATGATGTTTGAATTCGATTGAATAATGTTTGACCATAAAAATCCATAGTTTAGTTGGTTGTGGTATCTGATTGGGATGCAGAATACTTTTTATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTa                                                                                                                                                                                                                            >  1:502011/1‑249 (MQ=255)                         tAAGTTTGACGCAATATGACCGTTTTTTTCCGCTTGCTTACGTCGGCGGATGATATGATGTTTGAATTCGATTGAATAATGTTTGACCATAAAAATCCATAGTTTAGTTGGTTGTGGTATCTGATTGGGATGCAGAATACTTTTTATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGaa                                                                                                                                                                                                                      <  1:94844/250‑1 (MQ=255)                         tAAGTTTGACGCAATATGACCGTTTTTTTCCGCTTGCTTACGTCGGCGGATGATATGATGTTTGAATTCGATTGAATAATGTTTGACCATAAAAATCCATAGTTTAGTTGGTTGTGGTATCTGATTGGGATGCAGAATACTTTTTATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGaa                                                                                                                                                                                                                      <  1:694168/250‑1 (MQ=255)                         tAAGTTTGACGCAATATGACCGTTTTTTTCCGCTTGCTTACGTCGGCGGATGATATGATGTTTGAATTCGATTGAATAATGTTTGACCATAAAAATCCATAGTTTAGTTGGTTGTGGTATCTGATTGGGATGCAGAATACTTTTTATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGaa                                                                                                                                                                                                                      <  1:72292/250‑1 (MQ=255)                             tttGACGCAATATGACCGTTTTTTTCCGCTTGCTTACGTCGGCGGATGATATGATGTTTGAATTCGATTGAATAATGTTTGACCATAAAAATCCATAGTTTAGTTGGTTGTGGTATCTGATTGGGATGCAGAATACTTTTTATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCAt                                                                                                                                                                                                                 <  1:773468/251‑1 (MQ=255)                                 aCGCAATATGACCGTTTTTTTCCGCTTGCTTACGTCGGCGGATGATATGATGTTTGAATTCGATTGAATAATGTTTGACCATAAAAATCCATAGTTTATTTGGTTGTGGTATCTGATTGGGATGCAGAATACTTTTTATTCAATCTAATCAAACTTAAAGAGCCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAGTAATCACGCCTCGGCTTGTACTGGAATGCATAAAc                                                                                                                                                                                                             <  1:899683/251‑1 (MQ=255)                                    cAATATGACCGTTTTTTTCCGCTTGCTTACGTCGGCGGATGATATGATGTTTGAATTCGATTGAATAATGTTTGACCATAAAAATCCATAGTTTAGTTGGTTGTGGTATCTGATTGGGATGCAGAATACTTTTTATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAAc                                                                                                                                                                                                             <  1:236526/248‑1 (MQ=255)                                              gTTTTTTTCCGCTTGCTTACGTCGGCGGATGATATGATGTTTGAATTCGATTGAATAATGTTTGACCATAAAAATCCATAGTTTAGTTGGTTGTGGTATCTGATTGGGATGCAGAATACTTTTTATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGAc                                                                                                                                                                                                                                                    <  1:543839/199‑1 (MQ=255)                                                     tCCGCTTGCTTACGTCGGCGGATGATATGATGTTTGAATTCGATTGAATAATGTTTGACCATAAAAATCCATAGTTTAGTTGGTTGTGGTATCTGATTGGGATGCAGAATACTTTTTATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATAc                                                                                                                                                                                         <  1:816400/251‑1 (MQ=255)                                                         cttgcttACGTCGGCGGATGATATGATGTTTGAATTCGATTGAATAATGTTTGACCATAAAAATCCATAGTTTAGTTGGTTGTGGTATCTGATTGGGATGCAGAATACTTTTTATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGcc                                                                                                                                                                                      <  1:376468/250‑1 (MQ=255)                                                         cttgcttACGTCGGCGGATGATATGATGTTTGAATTCGATTGAATAATGTTTGACCATAAAAATCCATAGTTTAGTTGGTTGTGGTATCTGATTGGGATGCAGAATACTTTTTATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGcc                                                                                                                                                                                      <  1:765166/250‑1 (MQ=255)                                                           tgcttACGTCGGCGGATGATATGATGTTTGAATTCGATTGAATAATGTTTGACCATAAAAATCCATAGTTTAGTTGGTTGTGGTATCTGATTGGGATGCAGAATACTTTTTATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTg                                                                                                                                                                                                           <  1:537407/227‑1 (MQ=255)                                                                       cggATGATATGATGTTTGAATTCGATTGAATAATGTTTGACCATAAAAATCCATAGTTTAGTTGGTTGTGGTATCTGATTGGGATGCAGAATACTTTTTATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGCCTACATGATTATTTc                                                                                                                                                                        <  1:705674/250‑1 (MQ=255)                                                                           tgatatgatGTTTGAATTCGATTGAATAATGTTTGACCATAAAAATCCATAGTTTAGTTGGTTGTGGTATCTGATTGGGATGCAGAATACTTTTTATTCAATCTAATCAATCTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCa                                                                                                                                                                                                                                          <  1:267213/180‑1 (MQ=255)                                                                           tgatatgatGTTTGAATTCGATTGAATAATGTTTGACCATAAAAATCCATAGTTTAGTTGGTTGTGGTATCTGATTGGGATGCAGAATACTTTTTATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGCCTACATGa                                                                                                                                                                               >  1:835903/1‑239 (MQ=255)                                                                            gatatgatGTTTGAATTCGATTGAATAATGTTTGACCATAAAAATCCATAGTTTAGTTGGTTGTGGTATCTGATTGGGATGCAGAATACTTTTTATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGaa                                                                                                                                                                                                                      <  1:353588/199‑1 (MQ=255)                                                                            gatatgatGTTTGAATTCGATTGAATAATGTTTGACCATAAAAATCCATAGTTTAGTTGGTTGTGGTATCTGATTGGGATGCAGAATACTTTTTATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAAc                                                                                                                                                                                                             <  1:317398/208‑1 (MQ=255)                                                                            gatatgatGTTTGAATTCGATTGAATAATGTTTGACCATAAAAATCCATAGTTTAGTTGGTTGTGGTATCTGATTGGGATGCAGAATACTTTTTATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATAc                                                                                                                                                                                         <  1:436864/228‑1 (MQ=255)                                                                             atatgatGTTTGAATTCGATTGAATAATGTTTGACCATAAAAATCCATAGTTTAGTTGGTTGTGGTATCTGATTGGGATGCAGAATACTTTTTATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGCCTACATg                                                                                                                                                                                >  1:153652/1‑236 (MQ=255)                                                                                   tgTTTGAATTCGATTGAATAATGTTTGACCATAAAAATCCATAGTTTAGTTGGTTGTGGTATCTGATTGGGATGCAGAATACTTTTTATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGCCTACATGATTATTTCTACGGAAAAAAAc                                                                                                                                                           <  1:724202/251‑1 (MQ=255)                                                                                                  gAATAATGTTTGACCATAAAAATCCATAGTTTAGTTGGTTGTGGTATCTGATTGGGATGCAGAATACTTTTTATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGCCTACATGATTATTTCTACGGAAAAAAACCAAATCGCCCACttt                                                                                                                                            >  1:234727/1‑251 (MQ=255)                                                                                                       atGTTTGACCATAAAAATCCATAGTTTAGTTGGTTGTGGTATCTGATTGGGATGCAGAATACTTTTTATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGCCTACATGATTATTTCTACGGAAAAAAACCAAATCGCCCACTTTCAGtt                                                                                                                                       <  1:450364/251‑1 (MQ=255)                                                                                                       atGTTTGACCATAAAAATCCATAGTTTAGTTGGTTGTGGTATCTGATTGGGATGCAGAATACTTTTTATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGCCTACATGATTATTTCTACGGAAAAAAACCAAATCGCCCACTTTCAGtt                                                                                                                                       <  1:657697/251‑1 (MQ=255)                                                                                                               ccATAAAAATCCATAGTTTAGTTGGTTGTGGTATCTGATTGGGATGCAGAATACTTTTTATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGCCTACATGATTATTTCTACGGAAAAAAACCAAATCGCCCACTTTCAGTTctttcttt                                                                                                                               <  1:215231/251‑1 (MQ=255)                                                                                                                 aTAAAAATCCATAGTTTAGTTGGTTGTGGTATCTGATTGGGATGCAGAATACTTTTTATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGAc                                                                                                                                                                                                                                                    <  1:681292/132‑1 (MQ=255)                                                                                                                    aaaaTCCATAGTTTAGGTGGTTGTGGTATCTGATTGGGATGCAGAATACTTTTTATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGCCTACATGATTATTTCTACGGAAAAAAACCAAATCGCCCACTTTCAGTTCTTTCTTTTGAAt                                                                                                                          <  1:513978/251‑1 (MQ=255)                                                                                                                              gtttagttGGTTGTGGTATCTGATTGGGATGCAGAATACTTTTTATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGCCTACATGATTATTTCTACGGAAAAAAACCAAATCGCCCACTTTCAGTTCTTTCTTTTGAATCGCTTTACCg                                                                                                                >  1:374286/1‑251 (MQ=255)                                                                                                                              gtttagttGGTTGTGGTATCTGATTGGGATGCAGAATACTTTTTATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGCCTACATGATTATTTCTACGGAAAAAAACCAAATCGCCCACTTTCAGTTCTTTCTTTTGAATCGCTTTACCg                                                                                                                >  1:279782/1‑251 (MQ=255)                                                                                                                              gtttagttGGTTGTGGTATCTGATTGGGATGCAGAATACTTTTTATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGCCTACATGATTATTTCTACGGAAAAAAACCAAATCGCCCACTTTCAGTTCTTTCTTTTGAATCGCTTTACCg                                                                                                                >  1:678161/1‑251 (MQ=255)                                                                                                                              gtttagttGGTTGTGGTATCTGATTGGGATGCAGAATACTTTTTATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGCCTACATGATTATTTCTACGGAAAAAAACCAAATCGCCCACTTTCAGTTCTTTCTTTTGAATCGCTTTACCg                                                                                                                >  1:651857/1‑251 (MQ=255)                                                                                                                              gtttagttGGTTGTGGTATCTGATTGGGATGCAGAATACTTTTTATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGCCTACATGATTATTTCTACGGAAAAAAACCAAATCGCCCACTTTCAGTTCTTTCTTTTGAATCGCTTTACCg                                                                                                                >  1:220648/1‑251 (MQ=255)                                                                                                                                       gttgTGGTATCTGATTGGGATGCAGAATACTTTTTATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGaa                                                                                                                                                                                                                      >  1:774427/1‑140 (MQ=255)                                                                                                                                        ttgTGGTATCTGATTGGGATGCAGAATACTTTTTATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGCCTACATGATTATTTCTACGGAAAAAAACCAAATCGCCCACTTTCAGTTCTTTCTTTTGAATCGCTTTACCGACATAACGc                                                                                                       <  1:139891/250‑1 (MQ=255)                                                                                                                                             gTATCTGATTGGGATGCAGAATACTTTTTATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGCCTACATGATTATTTCTACGGAAAAAAACCAAATCGCCCACTTTCAGTTCTTTCTTTTGAATCGCTTTACCg                                                                                                                <  1:76873/236‑1 (MQ=255)                                                                                                                                                          aTGCAGAATACTTTTTATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGCCTACATGATTATTTCTACGGAAAAAAACCAAATCGCCCACTTTCAGTTCTTTCTTTTGAATCGCTTTACCGACATAACGCTGTTCGGAAGTCGAAcgcg                                                                                    >  1:347694/1‑251 (MQ=255)                                                                                                                                                            gCAGAATACTTTTTATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGCCTACAt                                                                                                                                                                                 <  1:226873/156‑1 (MQ=255)                                                                                                                                                                  caaacattATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGCCTACATGATTATTTCTACGGAAAAAAACCAAATCGCCCACTTTCAGTTCTTTCTTTTGAATCGCTTTACCGACATAACGCTGTTCGGAAGTCGAACGCGGCAATGTc                                                                            <  1:380264/245‑1 (MQ=255)                                                                                                                                                                    cTTTTTATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGt                                                                                                                                                                                                      >  1:79006/1‑127 (MQ=255)                                                                                                                                                                      ttttATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGCCTACATGATTATTTCTACGGAAAAAAACCAAATCGCCCACTTTCAGTTCTTTCTTTTGAATCGCTTTACCGACATAACGCTGTTCGGAAGTCGAACGCGGCAATGTCacac                                                                        <  1:355086/251‑1 (MQ=255)                                                                                                                                                                               aTCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGaa                                                                                                                                                                                                                      <  1:769906/100‑1 (MQ=255)                                                                                                                                                                               aTCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGCCTACATGATTATTTCTACGGAAAAAAACCAAATCGCCCACTTTCAGTTCTTTCTTTTGAATCGCTTTACCGACATAACGc                                                                                                       <  1:677673/211‑1 (MQ=255)                                                                                                                                                                                   aaTCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGCCTACATGATTATTTCTACGGAAAAAAACCAAATCGCCCACTTTCAGTTCTTTCTTTTGAATCGCTTTACCGACATAACGc                                                                                                       >  1:344684/1‑207 (MQ=255)                                                                                                                                                                                     tCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGCCTACATGATTATTTCTACGGAAAAAAACCAAATCGCCCACTTTCAGTTCTTTCTTTTGAATCGCTTTACCGACATAACGCTGTTCGGAAGTCGAACGCGGCAATGTCACACCAAACGCATCTaaaa                                                         <  1:509321/251‑1 (MQ=255)                                                                                                                                                                                        aaCTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGCCTACATGATTATTTCTACGGAAAAAAACCAAATCGCCCACTTTCAGTTCTTTCTTTTGAATCGCTTTACCGACATAACGCTGTTCGGAAGTCGAACGCGGCAATGTCACACCAAACGCATCTaaaa                                                         >  1:523957/1‑248 (MQ=255)                                                                                                                                                                                        aaCTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGCCTACATGATTATTTCTACGGAAAAAAACCAAATCGCCCACTTTCAGTTCTTTCTTTTGAATCGCTTTACCGACATAACGCTGTTCGGAAGTCGAACGCGGCAATGTCACACCAAACGCATCTAAAAAgg                                                      >  1:873537/1‑251 (MQ=255)                                                                                                                                                                                          cTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGCCTACATGATTATTTCTACGGAAAAAAACCAAATCGCCCACTTTCAGTTCTTTCTTTTGAATCGCTTTACCGACATAACGc                                                                                                       <  1:849099/200‑1 (MQ=255)                                                                                                                                                                                             aaaGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTg                                                                                                                                                                                                           <  1:84469/97‑1 (MQ=255)                                                                                                                                                                                                          ggATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGCCTACATGATTATTTCTACGGAAAAAAAc                                                                                                                                                           <  1:620671/132‑1 (MQ=255)                                                                                                                                                                                                               gggTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGCCTACATGATTATTTCTACGGAAAAAAACCAAATCGCCCACTTTCAGttc                                                                                                                                      <  1:277107/148‑1 (MQ=255)                                                                                                                                                                                                               gggTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGCCTACATGATTATTTCTACGGAAAAAAACCAAATCGCCCACTTTCAGTTCTTTCTTTTGAATCGCTTTAc                                                                                                                  <  1:347656/168‑1 (MQ=255)                                                                                                                                                                                                               gggTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGCCTACATGATTATTTCTACGGAAAAAAACCAAATCGCCCACTTTCAGTTCTTTCTTTTGAATCGCTTTACCGACATAACGCTGTTCGGAAGTCGAACGCGGCAATGTCACACCAAACGCATCTAAAAAggtg                                                    <  1:256286/230‑1 (MQ=255)                                                                                                                                                                                                               gggTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGCCTACATGATTATTTCTACGGAAAAAAACCAAATCGCCCACTTTCAGTTCTTTCTTTTGAATCGCTTTACCGACATAACGCTGTTCGGAAGTCGAACGCGGCAATGTCACACCAAACGCATCTAAAAAGGTGGTTTGCATAAAGc                                       >  1:772085/1‑243 (MQ=255)                                                                                                                                                                                                               gggTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGCCTACATGATTATTTCTACGGAAAAAAACCAAATCGCCCACTTTCAGTTCTTTCTTTTGAATCGCTTTACCGACATAACGCTGTTCGGAAGTCGAACGCGGCAATGTCACACCAAACGCATCTAAAAAGGTGGTTTGCATAAAGCCGGAAcaa                               >  1:771726/1‑251 (MQ=255)                                                                                                                                                                                                               gggTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGCCTACATGATTATTTCTACGGAAAAAAACCAAATCGCCCACTTTCAGTTCTTTCTTTTGAATCGCTTTACCGACATAACGCTGTTCGGAAGTCGAACGCGGCAATGTCACACCAAACGCATCTAAAAAGGTGGTTTGCATAAAGCCGGAAcaa                               >  1:634016/1‑251 (MQ=255)                                                                                                                                                                                                               gggTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGCCTACATGATTATTTCTACGGAAAAAAACCAAATCGCCCACTTTCAGTTCTTTCTTTTGAATCGCTTTACCGACATAACGCTGTTCGGAAGTCGAACGCGGCAATGTCACACCAAACGCATCTAAAAAGGTGGTTTGCATAAAGCCGGAAcaa                               >  1:373532/1‑251 (MQ=255)                                                                                                                                                                                                                 gTATACGTTCTCGCCCAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGCCTACATGATTATTTCTACGGAAAAAAACCAAATCGCCCACTTTCAGTTCTTTCTTTTGAATCGCTTTACCGACATAACGCTGTTCGGAAGTCGAACGCGGCAATGt                                                                             <  1:9528/203‑1 (MQ=255)                                                                                                                                                                                                                              cccAGTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGCCTACATGATTATTTCTACGGAAAAAAACCAAATCGCCCACTTTCAGTTCTTTCTTTTGAATCGCTTTACCGACATAACGCTGTTCGGAAGTCGAACGCGGCAATGTCACACCAAACGCATCTAAAAAGGTGGTTTGCATAAAGCCGGAACAATCAATGCCAtttttt                >  1:311982/1‑251 (MQ=255)                                                                                                                                                                                                                                  gTAATTGTCGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGCCTACATGATTATTTCTACGGAAAAAAACCAAATCGCCCACTTTCAGTTCTTTCTTTTGAATCGCTTTACCGACATAACGCTGTTCGGAAGCCGAACGCGGCAATGTCACACCAAACGCATCTAAAAAGGTGGTTTGCATAAAGCCGGAACAATAAATGCCATTTTTtgcgg            >  1:777883/1‑248 (MQ=255)                                                                                                                                                                                                                                          cGCTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGCCTACATGATTATTTCTACGGAAAAAAACCAAATCGCCCACTTTCAGTTCTTTCTTTTGAATCGCTTTACCGACATAACGCTGTTCGGAAGTCGAACGCGGCAATGTCACACCa                                                                      >  1:451889/1‑185 (MQ=255)                                                                                                                                                                                                                                            cTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGCCTACATGATTATTTCTACGGAAAAAAACCAAATCGCCCACTTTCAGttct                                                                                                                                     <  1:865286/120‑1 (MQ=255)                                                                                                                                                                                                                                            cTTAAGGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGCCTACATGATTATTTCTACGGAAAAAAACCAAATCGCCCACTTTCAGTTCTTTCTTTTGAATCGCTTTACCGACATAACGCTGTTCGGAAGTCGAACGCGGCAATGTCACACCAAACGCATCTAAAAAGGTGGTTTGCATAAAGCCGGAACAATCAATGCCATTTTTTGTGGTGCCGCCaaa  >  1:729667/1‑251 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                      TCTGGTAATCAGATTGCACGTGAGTAAGTTTGACGCAATATGACCGTTTTTTTCCGCTTGCTTACGTCGGCGGATGATATGATGTTTGAATTCGATTGAATAATGTTTGACCATAAAAATCCATAGTTTAGTTGGTTGTGGTATCTGATTGGGATGCAGAATACTTTTTATTCAATCTAATCAAACTTAAAGAACCCGACGGGATTGGGTATACGTTCTCGCCCAGTAATTGTCGCTTAAAGACTCAATAATCACGCCTCGGCTTGTACTGGAATGCATAAACTGATCGTTGCCGACGTATACGCCTACATGATTATTTCTACGGAAAAAAACCAAATCGCCCACTTTCAGTTCTTTCTTTTGAATCGCTTTACCGACATAACGCTGTTCGGAAGTCGAACGCGGCAATGTCACACCAAACGCATCTAAAAAGGTGGTTTGCATAAAGCCGGAACAATCAATGCCATTTTTTGTGGTGCCGCCAAA  >  1/1316587‑1317072

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑