Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,315,393	A→C	F217V (TTT→GTT)	unknown ←	Septum site‑determining protein MinC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,315,393	0	A	C	98.6%	200.4 / NA	73	F217V (TTT→GTT)	unknown	Septum site‑determining protein MinC
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base C (34/38);  total (34/39)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAAGATGCCATATCTGAAAAAATGGATGCCATGGCAACACAAGTCAATGAAATGAAAGATACAGCAGCTGAAGCAGTCAAAGATGCAAAAGACGCAGCGGCAGATAAAATGGCCGAAGTGAAAGATGCCGTATCTGAAAAAATGGGTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAAAAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGCTATTTACCGCCACGAATTCGGCATCTAAATATTGTGCATAAATAGATCCTGAACTGCTGTTGACGCCCGCCATGGCGCGCCCTAATAATTCGCCGTAAATGTGAATATTGCCGTCTGCCGCCACTTCGGCGCCATGCTCCACATCACCGTGAATGATTAAAT  >  1/1315148‑1315638                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      aaaGATGCCATATCTGAAAAAATGGATGCCATGGCAACACAAGTCAATGAAATGAAAGATACAGCAGCTGAAGAAGTCAAAGATGCAAAAGACGCAGCGGCAGATAAAATGGCCGAAGTGAAAGATGCCGTATCTGAAAAAATGGGTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTa                                                                                                                                                                                                                                                  >  1:161636/1‑251 (MQ=255)     aTGCCATATCTGAAAAAATGGATGCCATGGCAACACAAGTCAATGAAATGAAAGATACAGCAGCTGAAGCAGTCAAAGATGCAAAAGACGCAGCGGCAGATAAAATGGCCGAAGTGAAAGATGCCGTATCTGAAAAAATGGGTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAAtt                                                                                                                                                                                                                                               <  1:123766/250‑1 (MQ=255)       gCCATATCTGAAAAAATGGATGCCATGGCAACACAAGTCAATGAAATGAAAGATACAGCAGCTGAAGCAGTCAAAGATGCAAAAGACGCAGCGGCAGATAAAATGGCCGAAGTGAAAGATGCCGTATCTGAAAAAATGGGTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTAt                                                                                                                                                                                                                                            >  1:91846/1‑251 (MQ=255)       gCCATATCTGAAAAAATGGATGCCATGGCAACACAAGTCAATGAAATGAAAGATACAGCAGCTGAAGCAGTCAAAGATGCAAAAGACGCAGCGGCAGATAAAATGGCCGAAGTGAAAGATGCCGTATCTGAAAAAATGGGTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTAt                                                                                                                                                                                                                                            >  1:585084/1‑251 (MQ=255)                gAAAAAATGGATGCCATGGCAACACAAGTCAATGAAATGAAAGATACAGCAGCTGAAGCAGTCAAAGATGCAAAAGACGCAGCGGCAGATAAAATGGCCGAAGTGAAAGATGCCGTATCTGAAAAAATGGGTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCg                                                                                                                                                                                                                                   >  1:490878/1‑251 (MQ=255)                    aaaTGGATGCCATGGCAACACAAGTCAATGAAATGAAAGATACAGCAGCTGAAGCAGTCAAAGATGCAAAAGACGCAGCGGCAGATAAAATGGCCGAAGTGAAAGATGCCGTATCTGAAAAAATGGGTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCaaa                                                                                                                                                                                                                               >  1:550233/1‑251 (MQ=255)                          aTGCCATGGCAACACAAGTCAATGAAATGAAAGATACAGCAGCTGAAGCAGTCAAAGATGCAAAAGACGCAGCGGCAGATAAAATGGCCGAAGTGAAAGATGCCGTATCTGAAAAAATGGGTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATcc                                                                                                                                                                                                                          <  1:200916/250‑1 (MQ=255)                                tGGCAACACAAGTCAATGAAATGAAAGATACAGCAGCTGAAGCAGTCAAAGATGCAAAAGACGCAGCGGCAGATAAAATGGCCGAAGTGAAAGATGCCGTATCTGAAAAAATGGGTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAGAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCctgactga                                                                                                                                                                                                                   >  1:443880/1‑251 (MQ=255)                                   cAACACAAGTCAATGAAATGAAAGATACAGCAGCTGAAGCAGTCAAAGATGCAAAAGACGCAGCGGCAGATAAAATGGCCGAAGTGAAAGATGCCGTATCTGAAAAAATGGGTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACg                                                                                                                                                                                                                <  1:344961/251‑1 (MQ=255)                                            tCAATGAAATGAAAGATACAGCAGCTGAAGCAGTCAAAGATGCAAAAGACGCAGCGGCAGATAAAATGGCCGAAGTGAAAGATGCCGTATCTGAAAAAATGGGTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATaaaaa                                                                                                                                                                                                       <  1:802757/251‑1 (MQ=255)                                                    atgaaaGATACAGCAGCTGAAGCAGTCAAAGATGCAAAAGACGCAGCGGCAGATAAAATGGCCGAAGTGAAAGATGCCGTATCTGAAAAAATGGGTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTg                                                                                                                                                                                               >  1:172816/1‑251 (MQ=255)                                                      gaaaGATACAGCAGCTGAAGCAGTCAAAGATGCAAAAGACGCAGCGGCAGATAAAATGGCCGAAGTGAAAGATGCCGTATCTGAAAAAATGGGTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTGAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTggg                                                                                                                                                                                             >  1:226555/1‑251 (MQ=255)                                                         aGATACAGCAGCTGAAGCAGTCAAAGATGCAAAAGACGCAGCGGCAGATAAAATGGCCGAAGTGAAAGATGCCGTATCTGAAAAAATGGGTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGAtt                                                                                                                                                                                          >  1:114552/1‑251 (MQ=255)                                                           aTACAGCAGCTGAAGCAGTCAAAGATGCAAAAGACGCAGCGGCAGATAAAATGGCCGAAGTGAAAGATGCCGTATCTGAAAAAATGGGTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGAtttt                                                                                                                                                                                        >  1:726357/1‑251 (MQ=255)                                                              cagcagCTGAAGCAGTCAAAGATGCAAAAGACGCAGCGGCAGATAAAATGGCCGAAGTGAAAGATGCCGTATCTGAAAAAATGGGTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGAtttttt                                                                                                                                                                                      >  1:705783/1‑250 (MQ=255)                                                              cagcagCTGAAGCAGTCAAAGATGCAAAAGACGCAGCGGCAGATAAAATGGCCGAAGTGAAAGATGCCGTATCTGAAAAAATGGGTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGAtttttt                                                                                                                                                                                      >  1:860240/1‑250 (MQ=255)                                                                gcagcTGAAGCAGTCAAAGATGCAAAAGACGCAGCGGCAGATAAAATGGCCGAAGTGAAAGATGCCGTATCTGAAAAAATGGGTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCtt                                                                                                                                                                                   >  1:359840/1‑251 (MQ=255)                                                                  agcTGAAGCAGTCAAAGATGCAAAAGACGCAGCGGCAGATAAAATGGCCGAAGTGAAAGATGCCGTATCTGAAAAAATGGGTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTg                                                                                                                                                                                 >  1:19592/1‑251 (MQ=255)                                                                           aGTCAAAGATGCAAAAGACGCAGCGGCAGATAAAATGGCCGAAGTGAAAGATGCCGTATCTGAAAAAATGGGTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGaaaa                                                                                                                                                                        >  1:228366/1‑251 (MQ=255)                                                                            gTCAAAGATGCAAAAGACGCAGCGGCAGATAAAATGGCCGAAGTGAAAGATGCCGTATCTGAAAAAATGGGTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGaaaa                                                                                                                                                                        >  1:29492/1‑250 (MQ=255)                                                                            gTCAAAGATGCAAAAGACGCAGCGGCAGATAAAATGGCCGAAGTGAAAGATGCCGTATCTGAAAAAATGGGTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGaaaa                                                                                                                                                                        >  1:567367/1‑250 (MQ=255)                                                                               aaaGATGCAAAAGACGCAGCGGCAGATAAAATGGCCGAAGTGAAAGATGCCGTATCTGAAAAAATGGGTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGc                                                                                                                                                                    <  1:237589/251‑1 (MQ=255)                                                                               aaaGATGCAAAAGACGCAGCGGCAGATAAAATGGCCGAAGTGAAAGATGCCGTATCTGAAAAAATGGGTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGc                                                                                                                                                                    <  1:26626/251‑1 (MQ=255)                                                                                                      aGATAAAATGGCCGAAGTGAAAGATGCCGTATCTGAAAAAATGGGTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATaaaaa                                                                                                                                                                                                       >  1:414320/1‑193 (MQ=255)                                                                                                       gATAAAATGGCCGAAGTGAAAGATGCCGTATCTGAAAAAATGGGTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGCTATTTACCGCCACGAATTCGGCAt                                                                                                                                            >  1:641288/1‑251 (MQ=255)                                                                                                       gATAAAATGGCCGAAGTGAAAGATGCCGTATCTGAAAAAATGGGTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGCTATTTACCGCCACGAATTCGGCAt                                                                                                                                            >  1:709547/1‑251 (MQ=255)                                                                                                        aTAAAATGGCCGAAGTGAAAGATGCCGTATCTGAAAAAATGGGTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGCTATTTACCGCCACGAATTCGGc                                                                                                                                              <  1:371788/248‑1 (MQ=255)                                                                                                                gCCGAAGTGAAAGATGCCGTATCTGAAAAAATGGGTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGCTATTTACCGCCACGAATTCGGCATCTAAatat                                                                                                                                    <  1:312534/250‑1 (MQ=255)                                                                                                                gCCGAAGTGAAAGATGCCGTATCTGAAAAAATGGGTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGCTATTTACCGCCACGAATTCGGCATCTAAatat                                                                                                                                    <  1:789466/250‑1 (MQ=255)                                                                                                                gCCGAAGTGAAAGATGCCGTATCTGAAAAAATGGGTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAACAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGCTATTTACCGCCACGAATTCGGCATCTAAatat                                                                                                                                    <  1:580459/250‑1 (MQ=255)                                                                                                                    aaGTGAAAGATGCCGTATCTGAAAAAATGGGTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTc                                                                                                                                                                                                    <  1:846905/182‑1 (MQ=255)                                                                                                                      gTGAAAGATGCCGTATCTGAAAAAATGGGTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAAt                                                                                                                                                                                                                                                >  1:774524/1‑136 (MQ=255)                                                                                                                          aaGATGCCGTATCTGAAAAAATGGGTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACGGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGCTATTTACCGCCACGAATTCGGCATCTAAATATTGTGCATAAAt                                                                                                                         <  1:795832/251‑1 (MQ=255)                                                                                                                              tGCCGTATCTGAAAAAATGGGTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGCTATTTACCGCCACGAATTCGGCATCTAAATATTGTGCATAAATAGAt                                                                                                                     <  1:9100/251‑1 (MQ=255)                                                                                                                                ccGTATCTGAAAAAATGGGTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGCTATTTACCGCCACGAATTCGGCATCTAAATATTGTGCATAAATAGATcc                                                                                                                   <  1:300546/251‑1 (MQ=255)                                                                                                                                ccGTATCTGAAAAAATGGGTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGCTATTTACCGCCACGAATTCGGCATCTAAATATTGTGCATAAATAGATcc                                                                                                                   <  1:718345/251‑1 (MQ=255)                                                                                                                                    aTCTGAAAAAATGGGTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTc                                                                                                                                                                                     <  1:99267/181‑1 (MQ=255)                                                                                                                                     tCTGAAAAAATGGGTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGCTATTTACCGCCACGAATTCGGCATCTAAATATTGTGCATAAATAGATCCTGAAc                                                                                                              <  1:217713/251‑1 (MQ=255)                                                                                                                                      cTGAAAAAATGGGTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAAt                                                                                                                                                                                                      >  1:768654/1‑162 (MQ=255)                                                                                                                                               tGGGTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGCTATTTACCGCCACGAATTCGGCATCTAAATATTGTGCATAAATAGATCCTGAACTGCTGTTGAc                                                                                                    <  1:68872/251‑1 (MQ=255)                                                                                                                                                gggTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAAc                                                                                                                                                                       >  1:386285/1‑183 (MQ=255)                                                                                                                                                        ccGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGCTATTTACCGCCACGAATTCGGCATCTAAATATTGTGCATAAATAGATCCTGAACTGCTGTTGACGCCCGCCAt                                                                                           >  1:221023/1‑251 (MQ=255)                                                                                                                                                             acgcaaACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATaaaaa                                                                                                                                                                                                       <  1:467950/138‑1 (MQ=255)                                                                                                                                                                    cTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGCTATTTACCGCCACGAATTCGGCATCTAAATATTGTGCATAAAt                                                                                                                         <  1:780557/209‑1 (MQ=255)                                                                                                                                                                      gatgaAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGaaa                                                                                                                                                                         <  1:252575/159‑1 (MQ=255)                                                                                                                                                                       atgaaatgaaAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGCTATTTACCGCCACGAATTCGGCATCTAAATATTGTGCATAAATAGATCCTGAACTGCTGTTGACGCCCGCCATGGCGCGCCCtaataa                                                                            <  1:742983/251‑1 (MQ=255)                                                                                                                                                                       atgaaatgaaAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGCTATTTACCGCCACGAATTCGGCATCTAAATATTGTGCATAAATAGATCCTGAACTGCTGTTGACGCCCGCCATGGCGCGCCCtaataa                                                                            <  1:871629/251‑1 (MQ=255)                                                                                                                                                                         gaaatgaaaGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGCTATTTACCGCCACGAATTCGGCATCTAAatat                                                                                                                                    <  1:7014/193‑1 (MQ=255)                                                                                                                                                                         gaaatgaaaGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATAAAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGCTATTTACCGCCACGAATTCGGCATCTAAATATTGTGCATAAAt                                                                                                                         >  1:508640/1‑204 (MQ=255)                                                                                                                                                                           aatgaaaGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGCTATTTACCGCCACGAATTCGGCATCTAAatat                                                                                                                                    >  1:430996/1‑191 (MQ=255)                                                                                                                                                                            atgaaaGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGCTATTTACCGCCACGAATTCGGCATCTAAatat                                                                                                                                    <  1:201641/190‑1 (MQ=255)                                                                                                                                                                                aaGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGCTATTTACCGCCACGAATTCGGCATCTAAatat                                                                                                                                    <  1:622162/186‑1 (MQ=255)                                                                                                                                                                                     gcgGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGCTATTTACCGCCACGAATTCGGCATCTAAATATTGTGCATAAATAGATCCTGAACTGCTGTTGACGCCCGCCATGGCGCGCCCTAATAAtt                                                                          <  1:195311/239‑1 (MQ=255)                                                                                                                                                                                        gTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGCTATTTACCGCCACGAATTCGGCATCTAAATATTGTGCATAAATAGAt                                                                                                                     <  1:31543/193‑1 (MQ=255)                                                                                                                                                                                                    aCTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGCTATTTACCGCCACGAATTCGGCATCTAAATATTGTGCATAAATAGATCCTGAACTGCTGTTGACGCCCGCCATGGCGCGCCCTAATAATTCGCCGTAAATGTGAATATTGCCGt                                                  >  1:80489/1‑248 (MQ=255)                                                                                                                                                                                                       gAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGCTATTTACCGCCACGAATTCGGCATCTAAATATTGTGCATAAATAGATCCTGAACTGCTGTTGACGCCCGCCATGGCGCGCCCtaataa                                                                            >  1:535138/1‑219 (MQ=255)                                                                                                                                                                                                              aaTAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAACCGGCTATTTACCGCCACGAATTCGGCATCTAAATATTGTGCATAAATAGATCCTGAACTGCTGTTGACGCCCGCCATGGCGCGCCCtaataa                                                                            <  1:423870/212‑1 (MQ=255)                                                                                                                                                                                                                   aaaaGCAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGCTATTTACCGCCACGAATTCGGCATCTAAATATTGTGCATAAATAGATCCTGAACTGCTGTTGACGCCCGCCATGGCGCGCCCTAATAATTCGCCGTAAATGTGAATATTGCCGTCTGCCGCCACTTCGgcgc                                <  1:498523/251‑1 (MQ=255)                                                                                                                                                                                                                        cAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGCTATTTACCGCCACGAATTCGGCATCTAAatat                                                                                                                                    >  1:697162/1‑146 (MQ=255)                                                                                                                                                                                                                        cAGTCTAAACTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGCTATTTACCGCCACGAATTCGGCATCTAAATATTGTGCATAAATAGATCCTGAACTGCTGTTGACGCCCGCCATGGCGCGCCCTAATAATTCGCCGTAAAtg                                                               >  1:9000/1‑215 (MQ=255)                                                                                                                                                                                                                          gTCTAAACTGCTTTTTAAAGCGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGCTATTTACCGCCACGAATTCGGc                                                                                                                                              <  1:888920/134‑1 (MQ=255)                                                                                                                                                                                                                                aCTGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGCTATTTACCGCCACGAATTCGGCATCTAAATATTGTGCATAAATAGATCCTGAACTGCTGTTGACGCCCGCCATGGCGCGCCCTAATAATTCGCCGTAAATGTGAATATTGCCGTCTGCCGCCACTTCGGCGCCATGCTCCACATc                   <  1:733260/251‑1 (MQ=255)                                                                                                                                                                                                                                aCTGCTTTTTAAAACGGGGCAAAAACTAATTTATCCTTTTCCGCAAAAATCCTGGCTGAGCGAAATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGCTATTTGCCGCCACGAATTCGGCATCTAAATATTGTGCATAAATAGATCCTGAACTGCTGTTGACGCCCGCCATGGCGCGCCCTAATAATTCGCCGTAAATGTGAATATTGCCGTCTGCCGCCACTTCGGCGCCATGCTCCACATc                   <  1:145674/251‑1 (MQ=255)                                                                                                                                                                                                                                  tGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGCTATTTACCGCCACGAATTCGGCATCTAAATATTGTGCATAAATAGATCCTGAACTGCTGTTGACGCCCGCCATGGCGCGCCCTAATAATTCGCCGTAAATGTGAATATTGCCGTCTGCCGCCACTTCGGCGCCATGCTCCACATCAc                 <  1:617684/251‑1 (MQ=255)                                                                                                                                                                                                                                  tGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGCTATTTACCGCCACGAATTCGGCATCTAAATATTGTGCATAAATAGATCCTGAACTGCTGTTGACGCCCGCCATGGCGCGCCCTAATAATTCGCCGTAAATGTGAATATTGCCGTCTGCCGCCACTTCGGCGCCATGCTCCACATCAc                 <  1:685532/251‑1 (MQ=255)                                                                                                                                                                                                                                  tGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATAAAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGCTATTTACCGCCACGAATTCGGCATCTAAATATTGTGCATAAATAGATCCTGAACTGCTGTTGACGCCCGCCATGGCGCGCCCTAATAATTCGCCGTAAATGTGAATATTGCCGTCTGCCGCCACTTCGGCGCCATGCTCCACATCAc                 <  1:117547/251‑1 (MQ=255)                                                                                                                                                                                                                                  tGCTTTTTAAAACGGGGCAACAACTAATTTATCCTTTGCCGCAAAAACCCTGACTGAACGATATAAAAATTCAGTTGGAATTTTTTCTTTGTACAGAAAACGGCTATTTACCGCCACGAATTCGGCATCTAAATATTGTGCATAAATAGATCCTGAACTGCTGTTGACGCCCGCCATGGCGCGCCCTAATAATTCGCCGTAAATGTGAATATTGCCGTCTGCCGCCACTTCGGCGCCATGCTCCACATCAc                 <  1:784488/251‑1 (MQ=255)                                                                                                                                                                                                                                      ttttAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGCTATTTAc                                                                                                                                                             >  1:404160/1‑107 (MQ=255)                                                                                                                                                                                                                                        ttAAAACGGGGCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGCTATTTACCGCCACGAATTCGGCATCTAAATATTGTGCATAAATAGATCCTGAACTGCTGTTGACGCCCGCCATGGCGCGCCCTAATAATTCGCCGTAAATGTGAATATTGCCGTCTGCCGCCACTTCGGCGCCATGCTCCACATCACCGTGaa           <  1:241475/251‑1 (MQ=255)                                                                                                                                                                                                                                                gggCAACAACTAATTTATCCTTTTCCGCAAAAATCctgac                                                                                                                                                                                                                      >  1:418248/1‑40 (MQ=255)                                                                                                                                                                                                                                                gggCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGCTATTTACCGCCACGAATTCGGCATCTAAATATTGTGCATAAATAGATCCTGAACTGCTGTTGACGCCCGCCATGGCGCGCCCtaataa                                                                            <  1:596133/178‑1 (MQ=255)                                                                                                                                                                                                                                                gggCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGCTATTTACCGCCACGAATTCGGCATCTAAATATTGTGCATAAATAGATCCTGAACTGCTGTTGACGCCCGCCATGGCGCGCCCTAATAATTCGCCGTAAATGTGAATATTGCCGTCTGCCGCCACTTCGGCGCCATGCTc                         >  1:658146/1‑229 (MQ=255)                                                                                                                                                                                                                                                  gCAACAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGCTATTTACCGCCACGAATTCGGCATCTAAATATTGTGCATAAATAGATCCTGAACTGCTGTTGACGCCCGCCATGGCGCGCCCTAATAATTCGCCGTAAATGTGAATATTGCCGTCTGCCGCCACTTCGGCGCCATGCTCCACATCACCGTGAATGATTAAAt  >  1:752346/1‑250 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      AAAGATGCCATATCTGAAAAAATGGATGCCATGGCAACACAAGTCAATGAAATGAAAGATACAGCAGCTGAAGCAGTCAAAGATGCAAAAGACGCAGCGGCAGATAAAATGGCCGAAGTGAAAGATGCCGTATCTGAAAAAATGGGTGCCACCGCAACGCAAACTGATGAAATGAAAGACGCGGTAAAAAGCGAAACTGAAAGCAAATAAAAAAGCAGTCTAAACTGCTTTTTAAAACGGGGCAAAAACTAATTTATCCTTTTCCGCAAAAATCCTGACTGAACGATATAAAAATTCAGTTGGGATTTTTTCTTTGTACAGAAAACGGCTATTTACCGCCACGAATTCGGCATCTAAATATTGTGCATAAATAGATCCTGAACTGCTGTTGACGCCCGCCATGGCGCGCCCTAATAATTCGCCGTAAATGTGAATATTGCCGTCTGCCGCCACTTCGGCGCCATGCTCCACATCACCGTGAATGATTAAAT  >  1/1315148‑1315638

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑