Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,308,806	C→T	intergenic (‑686/+89)	unknown ← / ← unknown	Uncharacterized protein HI1720/UDP‑N‑acetylglucosamine kinase (EC 2.7.1.176), Zeta toxin

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,308,806	0	C	T	100.0%	94.2 / NA	36	intergenic (‑686/+89)	unknown/unknown	Uncharacterized protein HI1720/UDP‑N‑acetylglucosamine kinase (EC 2.7.1.176), Zeta toxin
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (20/16);  total (20/16)

TGACTAGTACTACGACCAAGGCGATCTAATTTTTTACTACAACGGTACTCCCTTATCGCACGAAATTTAATAATTCATCAAGTCTTGCACGGTTTTTAGCTTTTGAACCAGAATTCTTGCCTGTAAAAATTTTAAAACAACCTCCATTTTTAAGTTGTTTAATC‑TGCTCTTCATAATTTTGCCAAACGGTACTGGCACGGGTGGATCCAACAAGCCCGATCTATACCTCTTAAAATCCACTAGCTCTTAAAAACTTTGAAGTTTATGTAAATGGGAATTTGATATTTCCATCTAATATCTCAATTTAAAAATATCCTACCCTATTTTATCTCTTATAGCTTGAATTTTATTGAGTGATTTTCAGTAGAGATTGATTGACTTTTCTCACTTAAATTTTGCTTTACAGCTTCAACATTCTTTTTAAAGTCTCTTGAAGACTCAAGT  >  1/1308563‑1309006                                                                                                                                                                                                                                                     |                                                                                                                                                                                                         actacgaccaaggcgatctaattttttactacaacggtactcccttatcgcacgaaatttaataattcatcAATTCATCAAGTCTTGCACGGTTTTTAGCTTTTGAACCAGAATTCTTGCCTGTAAAAATTTTAAAACAACCTCCATTTTTAAGTTGTTTAATC‑TGCTCTTCATAATTTTGCCAAACGGTACTGGCACGGGTGGATCCAACAAGCCCGATCTATACCTCTTAAAATCCACTAGTTCTTaaa                                                                                                                                                                                                   <  1:724740‑M2/180‑1 (MQ=255) actacgaccaaggcgatctaattttttactacaacggtactcccttatcgcacgaaatttaataattcatcAATTCATCAAGTCTTGCACGGTTTTTAGCTTTTGAACCAGAATTCTTGCCTGTAAAAATTTTAAAACAACCTCCATTTTTAAGTTGTTTAATC‑TGCTCTTCATAATTTTGCCAAACGGTACTGGCACGGGTGGATCCAACAAGCCCGATCTATACCTCTTAAAATCCACTAGTTCTTaaa                                                                                                                                                                                                   >  1:425202‑M2/72‑251 (MQ=255)    acgaccaaggcgatctaattttttactacaacggtactcccttatcgcacgaaatttaataattcatcAATTCATCAAGTCTTGCACGGTTTTTAGCTTTTGAACCAGAATTCTTGCCTGTAAAAATTTTAAAACAACCTCCATTTTTAAGTTGTTTAATC‑TGCTCTTCATAATTTTGCCAAACGGTACTGGCACGGGTGGATCCAACAAGCCCGATCTATACCTCTTAAAATCCACTAGTTCTTAAAAAc                                                                                                                                                                                                <  1:831041‑M2/183‑1 (MQ=255)      gaccaaggcgatctaattttttactacaacggtactcccttatcgcacgaaatttaataattcatcAATTCATCAAGTCTTGCACGGTTTTTAGCTTTTGAACCAGAATTCTTGCCTGTAAAAATTTTAAAACAACCTCCATTTTTAAGTTGTTTAATC‑TGCTCTTCATAATTTTGCCAAACGGTACTGGCACGGGTGGATCCAACAAGCCCGATCTATACCTCTTAAAATCCACTAGTTCTTAAAAACtt                                                                                                                                                                                              >  1:710039‑M2/67‑251 (MQ=255)             gcgatctaattttttactacaacggtactcccttatcgcacgaaatttaataattcatcAATTCATCAAGTCTTGCACGGTTTTTAGCTTTTGAACCAGAATTCTTGCCTGTAAAAATTTTAAAACAACCTCCATTTTTAAGTTGTTTAATC‑TGCTCTTCATAATTTTGCCAAACGGTACTGGCACGGGTGGATCCAACAAGCCCGATCTATACCTCTTAAAATCCACTAGTTCTTAAAAACTTTGAAg                                                                                                                                                                                         <  1:141852‑M2/190‑1 (MQ=255)                atctaattttttactacaacggtactcccttatcgcacgaaatttaataattcatcAATTCATCAAGTCTTGCACGGTTTTTAGCTTTTGAACCAGAATTCTTGCCTGTAAAAATTTTAAAACAACCTCCATTTTTAAGTTGTTTAATC‑TGCTCTTCATAATTTTGCCAAACGGTACTGGCACGGGTGGATCCAACAAGCCCGATCTATACCTCTTAAAATCCACTAGTTCTTAAAAACTTTGAAGTTTAt                                                                                                                                                                                    >  1:95618‑M2/57‑251 (MQ=255)                                caacggtactcccttatcgcacgaaatttaataattcatcAATTCATCAAGTCTTGCACGGTTTTTAGCTTTTGAACCAGAATTCTTGCCTGTAAAAATTTTAAAACAACCTCCATTTTTAAGTTGTTTAATC‑TGCTCTTCATAATTTTGCCAAACGGTACTGGCACGGGTGGATCCAACAAGCCCGATCTATACCTCTTAAAATCCACTAGTTCTTAAAAACTTTGAAGTTTATGTAAATGGGAATTTGa                                                                                                                                                                    <  1:848973‑M2/211‑1 (MQ=255)                                    ggtactcccttatcgcacgaaatttaataattcatcAATTCATCAAGTCTTGCACGGTTTTTAGCTTTTGAACCAGAATTCTTGCCTGTAAAAATTTTAAAACAACCTCCATTTTTAAGTTGTTTAATC‑TGCTCTTCATAATTTTGCCAAACGGTACTGGCACGGGTGGATCCAACAAGCCCGATCTATACCTCTTAAAATCCACTAGTTCTTAAAAACTTTGAAGTTTATGTAAATGGGAATTTGATAtt                                                                                                                                                                >  1:398455‑M2/37‑251 (MQ=255)                                        ctcccttatcgcacgaaatttaataattcatcAATTCATCAAGTCTTGCACGGTTTTTAGCTTTTGAACCAGAATTCTTGCCTGTAAAAATTTTAAAACAACCTCCATTTTTAAGTTGTTTAATC‑TGCTCTTCATAATTTTGCCAAACGGTACTGGCACGGGTGGATCCAACAAGCCCGATCTATACCTCTTAAAATCCACTAGTTCTTAAAAACTTTGAAGTTTATGt                                                                                                                                                                                  >  1:68508‑M2/33‑229 (MQ=255)                                          cccttatcgcacgaaatttaataattcatcAATTCATCAAGTCTTGCACGGTTTTTAGCTTTTGAACCAGAATTCTTGCCTGTAAAAATTTTAAAACAACCTCCATTTTTAAGTTGTTTAATC‑TGCTCTTCATAATTTTGCCAAACGGTACTGGCACGGGTGGATCCAACAAGCCCGATCTATACCTCTTAAAATCCACTAGTTCTTAAAAACTTTGAAg                                                                                                                                                                                         >  1:623182‑M2/31‑220 (MQ=255)                                          cccttatcgcacgaaatttaataattcatcAATTCATCAAGTCTTGCACGGTTTTTAGCTTTTGAACCAGAATTCTTGCCTGTAAAAATTTTAAAACAACCTCCATTTTTAAGTTGTTTAATC‑TGCTCTTCATAATTTTGCCAAACGGTACTGGCACGGGTGGATCCAACAAGCCCGATCTATACCTCTTAAAATCCACTAGTTCTTAAAAACTTTGAAGTTTATGTAAATGGGAATTTGat                                                                                                                                                                   >  1:139307‑M2/31‑242 (MQ=255)                                          cccttatcgcacgaaatttaataattcatcAATTCATCAAGTCTTGCACGGTTTTTAGCTTTTGAACCAGAATTCTTGCCTGTAAAAATTTTAAAACAACCTCCATTTTTAAGTTGTTTAATC‑TGCTCTTCATAATTTTGCCAAACGGTACTGGCACGGGTGGATCCAACAAGCCCGATCTATACCTCTTAAAATCCACTAGTTCTTAAAAACTTTGAAGTTTATGTAAATGGGAATTTGat                                                                                                                                                                   >  1:607913‑M2/31‑242 (MQ=255)                                                         atttaataattcatcAATTCATCAAGTCTTGCACGGTTTTTAGCTTTTGAACCAGAATTCTTGCCTGTAAAAATTTTAAAACAACCTCCATTTTTAAGTTGTTTAATC‑TGCTCTTCATAATTTTGCCAAACGGTACTGGCACGGGTGGATCCAACAAGCCCGATCTATACCTCTTAAAATCCACTAGTTCTTAAAAACTTTGAAGTTTATGTAAATGGGAAt                                                                                                                                                                        <  1:200847‑M2/207‑1 (MQ=255)                                                         atttaataattcatcAATTCATCAAGTCTTGCACGGTTTTTAGCTTTTGAACCAGAATTCTTGCCTGTAAAAATTTTAAAACAACCTCCATTTTTAAGTTGTTTAATC‑TGCTCTTCATAATTTTGCCAAACGGTACTGGCACGGGTGGATCCAACAAGCCCGATCTATACCTCTTAAAATCCACTAGTTCTTAAAAACTTTGAAGTTTATGTAAATGGGAATTTGATATTTCCATCTAATATCTCAATTTa                                                                                                                                           >  1:361678‑M2/16‑251 (MQ=255)                                                            taataattcatcAATTCATCAAGTCTTGCACGGTTTTTAGCTTTTGAACCAGAATTCTTGCCTGTAAAAATTTTAAAACAACCTCCATTTTTAAGTTGTTTAATCTTGCTCTTCATAATTTTGCCAAACGGTACTGGCACGGGTGGATCCAACAAGCCCGATCTATACCTCTTAAAATCCACTAGTTCTTAAAAACTTTGAAGTTTATGTAAATggg                                                                                                                                                                           >  1:1143‑M2/13‑217 (MQ=255)                                                             aataattcatcAATTCATCAAGTCTTGCACGGTTTTTAGCTTTTGAACCAGAATTCTTGCCTGTAAAAATTTTAAAACAACCTCCATTTTTAAGTTGTTTAATC‑TGCTCTTCATAATTTTGCCAAACGGTACTGGCACGGGTGGATCCAACAAGCCCGATCTATACCTCTTAAAATCCACTAGTTCTTAAAAACTTTGAAGTTTATGTAAATGGGAATTTGATATTTCCATCTAATATCTCAATTTaaaaa                                                                                                                                       <  1:749897‑M2/240‑1 (MQ=255)                                                                  ttcatcAATTCATCAAGTCTTGCACGGTTTTTAGCTTTTGAACCAGAATTCTTGCCTGTAAAAATTTTAAAACAACCTCCATTTTTAAGTTGTTTAATC‑TGCTCTTCATAATTTTGCCAAACGGTACTGGCACGGGTGGATCCAACAAGCCCGATCTATACCTCTTAAAATCCACTAGTTCTTAAAAACTTTGAAGTTTATGTAAATGGGAATTTGATATTTCCATCTAATATCTCAATTTAAAAATATcc                                                                                                                                  <  1:496949‑M2/245‑1 (MQ=255)                                                                    catcAATTCATCAAGTCTTGCACGGTTTTTAGCTTTTGAACCAGAATTCTTGCCTGTAAAAATTTTAAAACAACCTCCATTTTTAAGTTGTTTAATC‑TGCTCTTCATAATTTTGCCAAACGGTACTGGCACGGGTGGATCCAACAAGCCCGATCTATACCTCTTAAAATCCACTAGTTCTTAAAAACTTTGAAGTTTATGTAAATGGGAATTTGATATTTCCATCTAATATCTCAATTTAAAAATATCCTa                                                                                                                                >  1:79870‑M2/5‑251 (MQ=255)                                                                       cAATTCATCAAGTCTTGCACGGTTTTTAGCTTTTGAACCAGAATTCTTGCCTGTAAAAATTTTAAAACAACCTCCATTTTTAAGTTGTTTAATC‑TGCTCTTCATAATTTTGCCAAACGGTACTGGCACGGGTGGATCCAACAAGCCCGATCTATACCTCTTAAAATCCACTAGTTCTTAAAAACTTTGAAGTTTATGTAAATGGGAATTTGATATTTCCATCTAATATCTCAATTTAAAAATATCCTAccc                                                                                                                             <  1:261436‑M2/250‑1 (MQ=255)                                                                       cAATTCATCAAGTCTTGCACGGTTTTTAGCTTTTGAACCAGAATTCTTGCCTGTAAAAATTTTAAAACAACCTCCATTTTTAAGTTGTTTAATC‑TGCTCTTCATAATTTTGCCAAACGGTACTGGCACGGGTGGATCCAACAAGCCCGATCTATACCTCTTAAAATCCACTAGTTCTTAAAAACTTTGAAGTTTATGTAAATGGGAATTTGATATTTCCATCTAATATCTCAATTTAAAAATATCCTAccc                                                                                                                             <  1:729828‑M2/250‑1 (MQ=255)                                                                           tcatcaAGTCTTGCACGGTTTTTAGCTTTTGAACCAGAATTCTTGCCTGTAAAAATTTTAAAACAACCTCCATTTTTAAGTTGTTTAATC‑TGCTCTTCATAATTTTGCCAAACGGTACTGGCACGGGTGGATCCAACAAGCCCGATCTATACCTCTTAAAATCCACTAGTTCTTAAAAACTTTGAAGTTTATGTAAATGGGAAtt                                                                                                                                                                       <  1:821273/205‑1 (MQ=255)                                                                                  gTCTTGCACGGTTTTTAGCTTTTGAACCAGAATTCTTGCCTGTAAAAATTTTAAAACAACCTCCATTTTTAAGTTGTTTAATC‑TGCTCTTCATAATTTTGCCAAACGGTACTGGCACGGGTGGATCCAACAAGCCCGATCTATACCTCTTAAAATCCACTAGTTCTTAAAAACTTTGAAGTTTATGTAAATGGGAATTTGATATTTc                                                                                                                                                              >  1:303647/1‑207 (MQ=255)                                                                                     ttGCACGGTTTTTAGCTTTTGAACCAGAATTCTTGCCTGTAAAAATTTTAAAACAACCTCCATTTTTAAGTTGTTTAATC‑TGCTCTTCATAATTTTGCCAAACGGTACTGGCACGGGTGGATCCAACAAGCCCGATCTATACCTCTTAAAATCCACTAGTTCTTAAAAACTTTGAAGTTTATGt                                                                                                                                                                                  >  1:411145/1‑184 (MQ=255)                                                                                        cACGGTTTTTAGCTTTTGAACCAGAATTCTTGCCTGTAAAAATTTTAAAACAACCTCCATTTTTAAGTTGTTTAATC‑TGCTCTTCATAATTTTGCCAAACGGTACTGGCACGGGTGGATCCAACAAGCCCGATCTATACCTCTTAAAATCCACTAGTTCTTAAAAACTTTGAAGTTTATGTAAATGGGAATTTGATATTTCCATCTAATATCTCAATTTAAAAATATCCTACCCTATTTTATCTCTTATAg                                                                                                            <  1:381859/251‑1 (MQ=255)                                                                                          cGGTTTTTAGCTTTTGAACCAGAATTCTTGCCTGTAAAAATTTTAAAACAACCTCCATTTTTAAGTTGTTTAATC‑TGCTCTTCATAATTTTGCCAAACGGTACTGGCACGGGTGGATCCAACAAGCCCGATCTATACCTCTTAAAATCCACTAGTTCTTAAAAACTTTGAAGTTTATGTAAATGGGAATTTGATATTTCCATCTAATATCTCAATTTAAAAATATCCTACCCTATTTTATCTCTTATAGCt                                                                                                          <  1:826216/251‑1 (MQ=255)                                                                                            gTTTTTCGCTTTTGAACCAGAATTCTTGCCTGTAAAAATTTTAAAACAACCTCCATTTTTAAGTTGTTTAATC‑TGCTCTTCATAATTTTGCCAAACGGTACTGGCACGGGTGGATCCAACAAGCCCGATCTATACCTCTTAACATCCACTAGTTCTTAAAAACTTTGAAGTTTATGt                                                                                                                                                                                  >  1:407680/1‑177 (MQ=255)                                                                                                                 aTTCTTGCCTGTAAAAATTTTAAAACAACCTCCATTTTTAAGTTGTTTAATC‑TGCTCTTCATAATTTTGCCAAACGGTACTGGCACGGGTGGATCCAACAAGCCCGATCTATACCTCTTAAAATCCACTAGTTCTTAAAAACTTTGAAGTTTATGTAAATGGGAATTTGATATTTCCATCTAATATCTCAATTTAAAAATATCCTACCCTATTTTATCTCTTATAGc                                                                                                           <  1:871422/227‑1 (MQ=255)                                                                                                                 aTTCTTGCCTGTAAAAATTTTAAAACAACCTCCATTTTTAAGTTGTTTAATC‑TGCTCTTCATAATTTTGCCAAACGGTACTGGCACGGGTGGATCCAACAAGCCCGATCTATACCTCTTAAAATCCACTAGTTCTTAAAAACTTTGAAGCTTATGTAAATGGGAATTTGATATTTCCATCTAATATCTCAATTTAAAAATATCCTACCCTATTTTATCTCTTATAGCt                                                                                                          >  1:339906/1‑228 (MQ=255)                                                                                                                                                   tttttAAGTTGTTTAATC‑TGCTCTTCATAATTTTGCCAAACGGTACTGGCACGGGTGGATCCAACAAGCCCGATCTATACCTCTTAAAATCCACTAGTTCTTAAAAACTTTGAAGTTTATGTAAATGGGAATTTGATATTTc                                                                                                                                                              <  1:692471/142‑1 (MQ=255)                                                                                                                                                            tgttTAATC‑TGCTCTTCATAATTTTGCCAAACGGTACTGGCACGGGTGGATCCAACAAGCCCGATCTATACCTCTTAAAATCCACTAGTTCTTAAAAACTTTGAAGTTTATGTAAATGGGAATTTGATATTTCCATCTAATATCTCAATTTAAAAATATCCTACCCTATTTTATCTCTTATAGCTTGAATTTTATTGAGTGATTTTCAGTAGAGATTGATTGACTTTTCTCACTTAAATTTTGCTTTACAg                                        >  1:127221/1‑251 (MQ=255)                                                                                                                                                                       gCTCTTCATAATTTTGCCAAACGGTACTGGCACGGGTGGATCCAACAAGCCCGATCTATACCTCTTAAAATCCACTAGTTCTTAAAAACTTTGAAGTTTATGTAAATGGGAATTTGATATTTCCATCTAATATCTCAATTTaaaaa                                                                                                                                       <  1:553044/146‑1 (MQ=255)                                                                                                                                                                            tCATAATTTTGCCAAACGGTACTGGCACGGGTGGATCCAACAAGCCCGATCTATACCTCTTAAAATCCACTAGTTCTTAAAAACTTTGAAGTTTATGTAAATGGGAATTTGATATTTCCATCTAATATCTCAATTTAAAAATATCCTACCCTATTTTATCTCTTATAGc                                                                                                           >  1:427422/1‑169 (MQ=255)                                                                                                                                                                                                   ggCACGGGTGGATCCAACAAGCCCGATCTATACCTCTTAAAATCCACTAGTTCTTAAAAACTTTGAAGTTTATGTAAATGGGAATTTGATATTTCCATCTAATATCTCAATTTAAAAATATCCTACCCTATTTTATCTCTTATAGCTTGAATTTTATTGAGTGATTTTCAGTAGAGATTGATTGACTTTTCTCACTTAAATTTTGCTTTACAGCTTCAACATTCTTTTTAAAGTCTCTTGAAGACTCAAGt  >  1:167806/1‑251 (MQ=255)                                                                                                                                                                                                             gATCCAACAAGCCCGATCTATACCTCTTAAAATCCACTAGTTCTTAAAAACTTTGAAGTTTATGTAAATGGGAATTTGATATTTCCATCTAATATCTCAATTTAAAAATATCCTACCCTATTTTATCTCTTATAGCt                                                                                                          >  1:690981/1‑137 (MQ=255)                                                                                                                                                                                                             gATCCAACAAGCCCGATCTATACCTCTTAAAATCCACTAGTTCTTAAAAACTTTGAAGTTTATGTAAATGGGAATTTGATATTTCCATCTAATATCTCAATTTAAAAATATCCTACCCTATTTTATCTCTTATAGCTTGAATTTTATTGAGTGATTTTCAGTAGAGATTGATTGACTTTTCTCACTTAAATTTTGCTTTACAGCTTCAACATTCTTTTTAAAGTCTCTTGAAg          >  1:617257/1‑233 (MQ=255)                                                                                                                                                                                                                              cTATACCTCTTAAAATCCACTAGTTCTTAAAAACTTTGAAGTTTATGTAAATGGGAATTTGATATTTCCATCTAATATCTCAATTTAAAAATATCCTACCCTATTTTATCTCTTATAGCTTGAATTTTATTg                                                                                              <  1:281045/132‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                         TGACTAGTACTACGACCAAGGCGATCTAATTTTTTACTACAACGGTACTCCCTTATCGCACGAAATTTAATAATTCATCAAGTCTTGCACGGTTTTTAGCTTTTGAACCAGAATTCTTGCCTGTAAAAATTTTAAAACAACCTCCATTTTTAAGTTGTTTAATC‑TGCTCTTCATAATTTTGCCAAACGGTACTGGCACGGGTGGATCCAACAAGCCCGATCTATACCTCTTAAAATCCACTAGCTCTTAAAAACTTTGAAGTTTATGTAAATGGGAATTTGATATTTCCATCTAATATCTCAATTTAAAAATATCCTACCCTATTTTATCTCTTATAGCTTGAATTTTATTGAGTGATTTTCAGTAGAGATTGATTGACTTTTCTCACTTAAATTTTGCTTTACAGCTTCAACATTCTTTTTAAAGTCTCTTGAAGACTCAAGT  >  1/1308563‑1309006

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑