Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,298,716	T→C	intergenic (+275/+14)	unknown → / ← unknown	Mobile element protein/tRNA‑Trp‑CCA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,298,716	0	T	C	100.0%	214.2 / NA	74	intergenic (+275/+14)	unknown/unknown	Mobile element protein/tRNA‑Trp‑CCA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/35);  total (39/35)

TCAGTTTAAGGACATTGATGAATCAGAATGGATGATACATGATATATTCGTTATTATACCTAGGAGCAAATTAAGCTCGGGTTAGACGATTTAAACCCTGTGCAATATAGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTTAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGAAAAGGTTACAAAAGTTGGCGGAATGGACGGGACTCGAACCCGCGACCCCCTGCGTGACAGGCAGGTATTCTAACCAGCTGAACTACCACTCCAGAATGAGAATTAGCAGTGACCTACTCTCACATAGG  >  1/1298483‑1298952                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                             tCAGTTTAAGGACATTGATGAATCAGAATGGATGATACATGATATATTCGTTATTATACCTAGGAGCAAATTAAGCTCGGGTTAGACGATTTAAACCCTGTGCAGTATAGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGc                                                                                                                                                                                                                             <  1:82699/251‑1 (MQ=255)            aCATTGATGAATCAGAATGGATGATACATGATATATTCGTTATTATACCTAGGAGCAAATTAAGCTCGGGTTAGACGATTTAAACCCTGTGCAGTATAGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAAt                                                                                                                                                                                                                                >  1:582428/1‑237 (MQ=255)                     aaTCAGAATGGATGATACATGATATATTCGTTATTATACCTAGGAGCAAATTAAGCTCGGGTTAGACGATTTAAACCCTGTGCAGTATAGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGaa                                                                                                                                                                                                          <  1:120143/250‑1 (MQ=255)                                   atACATGATATATTCGTTATTATACCTAGGAGCAAATTAAGCTCGGGTTAGACGATTTAAACCCTGTGCAGTATAGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGAt                                                                                                                                                                                                                                         <  1:135728/205‑1 (MQ=255)                                      cATGATATATTCGTTATTATACCTAGGAGCAAATTAAGCTCGGGTTAGACGATTTAAACCCTGTGCAGTATAGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAAt                                                                                                                                                                                                                                >  1:726918/1‑211 (MQ=255)                                          atatatTCGTTATTATACCTAGGAGCAGATTAAGCTCGGGTTAGACGATTTAAACCCTGTGCAGTATAGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGAt                                                                                                                                                                                                                                         >  1:823751/1‑198 (MQ=255)                                                 cGTTATTATACCTAGGAGCAAATTAAGCTCGGGTTAGACGATTTAAACCCTGTGCAGTATAGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACAcc                                                                                                                                                                                               >  1:585281/1‑233 (MQ=255)                                                   ttattaTACCTAGGAGCAAATTAAGCTCGGGTTAGACGATTTAAACCCTGTGCAGTATAGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTg                                                                                                                                                                             >  1:629780/1‑249 (MQ=255)                                                   ttattaTACCTAGGAGCAAATTAAGCTCGGGTTAGACGATTTAAACCCTGTGCAGTATAGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTg                                                                                                                                                                             >  1:311235/1‑249 (MQ=255)                                                     attatACCTAGGAGCAAATTAAGCTCGGGTTAGACGATTTAAACCCTGTGCAGTATAGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGct                                                                                                                                                                           >  1:348031/1‑249 (MQ=255)                                                                 aGCAAATTAAGCTCGGGTTAGACGATTTAAACCCTGTGCAGTATAGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAAtt                                                                                                                                                                  >  1:280074/1‑246 (MQ=255)                                                                 aGCAAATTAAGCTCGGGTTAGACGATTTAAACCCTGTGCAGTATAGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAAc                                                                                                                                                              <  1:132504/250‑1 (MQ=255)                                                                    aaaTTAAGCTCGGGTTAGACGATTTAAACCCTGTGCAGTATAGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAaca                                                                                                                                                                                                 <  1:852937/212‑1 (MQ=255)                                                                    aaaTTAAGCTCGGGTTAGACGATTTAAACCCTGTGCAGTATAGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACAcc                                                                                                                                                                                               >  1:551990/1‑214 (MQ=255)                                                                      aTTAAGCTCGGGTTAGACGATTTAAACCCTGTGCAGTATAGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGcc                                                                                                                                                        <  1:688874/251‑1 (MQ=255)                                                                                 gTTAGACGATTTAAACCCTGTGCAGTATAGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGgta                                                                                                                                              <  1:821521/250‑1 (MQ=255)                                                                                 gTTAGACGATTTAAACCCTGTGCAGTATAGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGgta                                                                                                                                              <  1:631181/250‑1 (MQ=255)                                                                                         aTTTAAACCCTGTGCAGTATAGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGgtatgtat                                                                                                                                         <  1:643098/247‑1 (MQ=255)                                                                                         aTTTAAACCCTGTGCAGTATAGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAAc                                                                                                                                      >  1:607186/1‑250 (MQ=255)                                                                                         aTTTAAACCCTGTGCAGTATAGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAAc                                                                                                                                      >  1:494588/1‑250 (MQ=255)                                                                                         aTTTAAACCCTGTGCAGTATAGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAAc                                                                                                                                      <  1:688894/250‑1 (MQ=255)                                                                                         aTTTAAACCCTGTGCAGTATAGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAAc                                                                                                                                      >  1:882174/1‑250 (MQ=255)                                                                                         aTTTAAACCCTGTGCAGTATAGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGACGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAAcc                                                                                                                                     <  1:413248/251‑1 (MQ=255)                                                                                          tttAAACCCTGTGCAGTATAGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGAAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAAc                                                                                                                                      >  1:456053/1‑249 (MQ=255)                                                                                               aCCCTGTGCAGTATAGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAGATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGaaaa                                                                                                                               >  1:881432/1‑251 (MQ=255)                                                                                                  cTGTGCAGTATAGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAAcc                                                                                                                                     <  1:465185/242‑1 (MQ=255)                                                                                                    gtgCAGTATAGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAac                                                                                                                                                                                                  >  1:335815/1‑179 (MQ=255)                                                                                                    gtgCAGTATAGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGgta                                                                                                                                              <  1:547344/231‑1 (MQ=255)                                                                                                    gtgCAGTATAGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGgta                                                                                                                                              <  1:735932/231‑1 (MQ=255)                                                                                                    gtgCAGTATAGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCt                                                                                                                                    <  1:32587/241‑1 (MQ=255)                                                                                                    gtgCAGTATAGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGAAAAGGTTa                                                                                                                          >  1:895590/1‑251 (MQ=255)                                                                                                      gcagtataGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAAc                                                                                                                                                                                                         >  1:711783/5‑170 (MQ=255)                                                                                                      gcagtataGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAac                                                                                                                                                                                                  >  1:730421/5‑177 (MQ=255)                                                                                                      gcagtataGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTAc                                                                                                                                                                       >  1:173794/5‑204 (MQ=255)                                                                                                      gcagtataGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGgtatgtat                                                                                                                                         >  1:114644/5‑234 (MQ=255)                                                                                                      gcagtataGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGgta                                                                                                                                              <  1:732301/225‑1 (MQ=255)                                                                                                      gcagtataGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGtata                                                                                                                                        <  1:45842/231‑1 (MQ=255)                                                                                                      gcagtataGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGAAAAGGTTACa                                                                                                                        >  1:379587/5‑251 (MQ=255)                                                                                                      gcagtataGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGAAAAGGTTACa                                                                                                                        >  1:607408/5‑251 (MQ=255)                                                                                                      gcagtataGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGAAAAGGTTACa                                                                                                                        >  1:160146/5‑251 (MQ=255)                                                                                                         gtataGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGAt                                                                                                                                                                                                                                         >  1:259967/2‑135 (MQ=255)                                                                                                         gtataGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCa                                                                                                                                                                     <  1:692549/202‑1 (MQ=255)                                                                                                         gtataGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGaaaa                                                                                                                               <  1:835972/240‑1 (MQ=255)                                                                                                         gtataGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGaaa                                                                                                                                >  1:507644/2‑240 (MQ=255)                                                                                                         gtataGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGACAAGGTTACaaaa                                                                                                                     >  1:705750/2‑251 (MQ=255)                                                                                                         gtataGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGAAAAGGTTACaaaa                                                                                                                     >  1:358485/2‑251 (MQ=255)                                                                                                         gtataGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGAAAAGGTTACaaaa                                                                                                                     >  1:320011/2‑251 (MQ=255)                                                                                                                gCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATGTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAAcc                                                                                                                                     <  1:424508/228‑1 (MQ=255)                                                                                                                   cAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGAAAAGGTTACaaa                                                                                                                      <  1:649439/240‑1 (MQ=255)                                                                                                                     atatTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGAAAAgg                                                                                                                             >  1:217707/1‑231 (MQ=255)                                                                                                                     atatTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGAAAAGGTTACAAAAGTTGGCGGAATg                                                                                                         >  1:311283/1‑251 (MQ=255)                                                                                                                     atatTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGAAAAGGTTACAAAAGTTGGCGGAATg                                                                                                         <  1:276823/251‑1 (MQ=255)                                                                                                                            gTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGAAAAGGTTACAAAAGTTGGCGGAATGGACGGGa                                                                                                  >  1:820363/1‑251 (MQ=255)                                                                                                                             ttATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTGTGAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGAAAAGGTTACAAAAGTTGGCGGAATGGACggg                                                                                                   <  1:126399/249‑1 (MQ=255)                                                                                                                                   gTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGAAAAGGTTACAAAAGTTGGCGGAATGGACGGGACTCGaa                                                                                            >  1:338181/1‑250 (MQ=255)                                                                                                                                                   tCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGAAAAGGTTACAAAAGTTGGCGGAATGGACGGGACTCGAACCCGCGACCCCCTGCGt                                                                           <  1:719626/251‑1 (MQ=255)                                                                                                                                                     aagtcgcTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGAAAAGGTTACAAAAGTTGGCGGAATGGACGGGACTCGAACCCGCGACCCCCTGCGTGa                                                                         <  1:14663/245‑1 (MQ=255)                                                                                                                                                                  aaTCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGAAAAGGTTACAAAAGTTGGCGGAATGGACGGGACTCGAACCCGCGACCCCCTGCGTGACAGGCAGGTATTc                                                            <  1:833322/251‑1 (MQ=255)                                                                                                                                                                     cTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAGCTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGAAAAGGTTACAAAAGTTGGCGGAATGGACGGGACTCGAACCCGCGACCCCCTGCGTGACAGGCAGGTATTCTaa                                                         <  1:379646/251‑1 (MQ=255)                                                                                                                                                                      tGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGAAAAGGTTACAAAAGTTGGCGGAATGGACGGGACTCGAACCCGCGACCCCCTGCGTGACAGGCAGGTATTCTAAc                                                        <  1:478370/251‑1 (MQ=255)                                                                                                                                                                        aTTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGAAAAGGTTACAAAAGTTGGCGGAATGGACGGGACTCGAACCCGCGACCCCCTGCGTGACAGGCAGGTATTCTAAcc                                                       <  1:417240/250‑1 (MQ=255)                                                                                                                                                                        aTTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGAAAAGGTTACAAAAGTTGGCGGAATGGACGGGACTCGAACCCGCGACCCCCTGCGTGACAGGCAGGTATTCTAAcc                                                       <  1:822897/250‑1 (MQ=255)                                                                                                                                                                           ttAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGAAAAGGTTACAAAAGTTGGCGGAATGGACGGGACTCGAACCCGCGACCCCCTGCGTGACAGGCAGGTATTCTAACCAg                                                     >  1:521414/1‑249 (MQ=255)                                                                                                                                                                                  aCAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGAAAAGATTACAAAAGTTGGCGGAATGGACGGGACTCGAACCCGCGACCCCCTGCGTGACAGGCAGGTATTCTAACCAGCTGAACTa                                             >  1:443007/1‑250 (MQ=255)                                                                                                                                                                                     gTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGAAAAGGTTACAAAAGTTGGCGGAATGGACGGGACTCGAACCCGCGACCCCCTGCGTGACAGGCAGGTATTCTAACCAGCTGAACTACCa                                          >  1:650979/1‑250 (MQ=255)                                                                                                                                                                                      ttttCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGAAAAGGTTACAAAAGTTGGCGGAATGGACGGGACTCGAACCCGCGACCCCCTGCGTGACAGGCAGGTATTCTAACCAGCTGAACTAc                                            <  1:162268/247‑1 (MQ=255)                                                                                                                                                                                                                 aaaCGGATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGAAAAGGTTACAAAAGTTGGCGGAATGGACGGGACTCGAACCCGCGACCCCCTGCGTGACAGGCAGGTATTCTAACCAGCTGAACTACCACTCCAGAATGAGAATTAGCAGTGACCTAc             <  1:809954/251‑1 (MQ=255)                                                                                                                                                                                                                      gATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGgtatg                                                                                                                                            >  1:650598/1‑119 (MQ=255)                                                                                                                                                                                                                      gATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGAAAAGGTTACAAAAGTTGGCGGAATGGACGGGACTCGAACCCGCGACCCCCTGCGTGACAGGCAGGTATTCTAACCAGCTGAACTACCACTCCAGAATGAGAATTAGCAGTGACCTACTCTca        >  1:106134/1‑251 (MQ=255)                                                                                                                                                                                                                      gATAGAGACAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGAAAAGGTTACAAAAGTTGGCGGAATGGACGGGACTCGAACCCGCGACCCCCTGCGTGACAGGCAGGTATTCTAACCAGCTGAACTACCACTCCAGAATGAGAATTAGCAGTGACCTACTCTca        >  1:427054/1‑251 (MQ=255)                                                                                                                                                                                                                           agaCAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGAAAAGGTTACAAAAGTTGGCGGAATGGACGGGACTCGAACCCGCGACCCCCTGCGTGACAGGCAGGTATTCTAACCAGCTGAACTACCACTCCAGAATGAGAATTAGCAGTGACCTACtctc         >  1:659346/1‑245 (MQ=255)                                                                                                                                                                                                                             aCAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTAc                                                                                                                                                                       <  1:477242/85‑1 (MQ=255)                                                                                                                                                                                                                             aCAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGAAAAGGTTACAAAAGTTGGCGGAATGGACGGGACTCGAACCCGCGACCCCCTGCGTGACAGGCAGGTATTCTAACCAGCTGAACTACCACTCCAGAATGAGAATTAGCAGTGACCTACTCTCACATAgg  <  1:412654/250‑1 (MQ=255)                                                                                                                                                                                                                             aCAATTTTCAGTTCAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGAAAAGGTTACAAAAGTTGGCGGAATGGACGGGACTCGAACCCGCGACCCCCTGCGTGACAGGCAGGTATTCTAACCAGCTGAACTACCACTCCAGAATGAGAATTAGCAGTGACCTACTCTCACATAg   <  1:391733/249‑1 (MQ=255)                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                             TCAGTTTAAGGACATTGATGAATCAGAATGGATGATACATGATATATTCGTTATTATACCTAGGAGCAAATTAAGCTCGGGTTAGACGATTTAAACCCTGTGCAATATAGAGCTCAATATTTAGTTATCTGTCCAACTTTTGGGGCTCAAATCACTTTTTTAATCTGATTTTAAAGTACAGTTTTCTTTTTTAAAGAAATTAGATTTAAAACGGATAGAGACAATTTTCAGTTTAGGATGAAGTAAATGGCAGGGGCGGAGAGTCTCGAACTCCCAACACCCGGTTTTGGAGACCGGTGCTCTACCAATTGAACTACGCCCCTATTGGTATGTATAACCTGAAAAGGTTACAAAAGTTGGCGGAATGGACGGGACTCGAACCCGCGACCCCCTGCGTGACAGGCAGGTATTCTAACCAGCTGAACTACCACTCCAGAATGAGAATTAGCAGTGACCTACTCTCACATAGG  >  1/1298483‑1298952

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑