Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,273,904	A→G	T668T (ACA→ACG)	unknown →	Alpha‑glucosidase (EC 3.2.1.20)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,273,904	0	A	G	100.0%	133.5 / NA	49	T668T (ACA→ACG)	unknown	Alpha‑glucosidase (EC 3.2.1.20)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (20/29);  total (20/29)

TTTGGAAAGCACATCAAGATAACGAACCTATATTGCGTCCAACTTTCCTTGATCATGAACATGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGCGGCGAAAAAATCACGGTAGATACACCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCGAGAATTATTAATTATGCCTTTTATTAATCAAGGAGAAAGAAATATCACAGTGTTTGATGATGATGGCGAAAGTTTTGATTATCTCAAAGGTAAATATTTAGAGCTTCACATTCATCTAAAATGCACGAACCAAACA  >  1/1273663‑1274132                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                     tttGGAAAGCACATCAAGATAACGAACCTATATTGCGTCCAACTTTCCTTGATCATGAACATGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGaa                                                                                                                                                                                                                             <  1:576328/251‑1 (MQ=255)  ttGGAAAGCACATCAAGATAACGAACCTATATTGCGTCCAACTTTCCTTGATCATGAACATGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGaaa                                                                                                                                                                                                                            >  1:884584/1‑251 (MQ=255)     gAAAGCACATCAAGATAACGAACCTATATTGCGTCCAACTTTCCTTGATCATGAACATGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAgg                                                                                                                                                                                                                          <  1:297128/250‑1 (MQ=255)       aaGCACATCAAGATAACGAACCTATATTGCGTCCAACTTTCCTTGATCATGAACATGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATc                                                                                                                                                                                                                       <  1:717835/251‑1 (MQ=255)                       cGAACCTATATTGCGTCCAACTTTCCTTGATCATGAACATGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAagag                                                                                                                                                                                                       >  1:329501/1‑251 (MQ=255)                        gAACCTATATTGCGTCCAACTTTCCTTGATCATGAACATGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAagag                                                                                                                                                                                                       >  1:1217/1‑250 (MQ=255)                           ccTATATTGCGTCCAACTTTCCTTGATCATGAACATGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGaa                                                                                                                                                                                                                             <  1:312282/225‑1 (MQ=255)                             tataTTGCGTCCAACTTTCCTTGATCATGAACATGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGaa                                                                                                                                                                                                 <  1:80330/251‑1 (MQ=255)                                            tttCCTTGATCATGAACATGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATgg                                                                                                                                                                                  <  1:91628/251‑1 (MQ=255)                                             ttCCTTGATCATGAACATGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTc                                                                                                                                                                                        >  1:556492/1‑244 (MQ=255)                                                    aTCATGAACATGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGaaaa                                                                                                                                                                          >  1:227299/1‑251 (MQ=255)                                                    aTCATGAACATGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGaaa                                                                                                                                                                           <  1:863613/250‑1 (MQ=255)                                                    aTCATGAACATGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAACTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGaaa                                                                                                                                                                           <  1:409313/250‑1 (MQ=255)                                                             aTGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATaaa                                                                                                                                                                 <  1:101042/251‑1 (MQ=255)                                                              tGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAag                                                                                                                                                                                                         >  1:113948/1‑210 (MQ=255)                                                               gATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATaaaaa                                                                                                                                                               >  1:258257/1‑251 (MQ=255)                                                                  aaaaaCACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATAc                                                                                                                                                            <  1:424429/251‑1 (MQ=255)                                                                    aaaCACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACAt                                                                                                                                                          <  1:638070/251‑1 (MQ=255)                                                                        acaTTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATc                                                                                                                                                      <  1:383631/251‑1 (MQ=255)                                                                        acaTTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATc                                                                                                                                                      <  1:772692/251‑1 (MQ=255)                                                                         caTTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAg                                                                                                                                                                                                >  1:406713/1‑208 (MQ=255)                                                                               cAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCtgatga                                                                                                                                                <  1:389906/250‑1 (MQ=255)                                                                                       gatgatTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCgaga                                                                                                                                       >  1:734413/1‑251 (MQ=255)                                                                                           atTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGaaa                                                                                                                                                                           <  1:140164/211‑1 (MQ=255)                                                                                                       gTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCGAGAATTATTAATTATGcc                                                                                                                        <  1:759786/250‑1 (MQ=255)                                                                                                            gATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTAt                                                                                                                                                       <  1:656139/214‑1 (MQ=255)                                                                                                                         cATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCGAGAATTATTAATTATGCCTTTTATTAATCAAGGagaa                                                                                                     >  1:771063/1‑251 (MQ=255)                                                                                                                         cATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCGAGAATTATTAATTATGCCTTTTATTAATCAAGGagaa                                                                                                     <  1:254421/251‑1 (MQ=255)                                                                                                                              gtgGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACAt                                                                                                                                                          <  1:150435/193‑1 (MQ=255)                                                                                                                              gtgGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCtgatga                                                                                                                                                >  1:620599/1‑203 (MQ=255)                                                                                                                                     aaCCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAg                                                                                                                                                                                                <  1:437119/148‑1 (MQ=255)                                                                                                                                           aTCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCGAGAATTATTAATTATGCCTTTTATTAATCAAGGAGAAAGAAATATCACAGTGttt                                                                                   >  1:647907/1‑251 (MQ=255)                                                                                                                                              aaaGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCGAGAATTATTAATTATGCCTTTTATTAATCAAGGAGAAAGAAATATCACAGTGTTtgat                                                                                <  1:522552/251‑1 (MQ=255)                                                                                                                                               aaGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCGAGAATTATTAATTATGCCTTTTATTAATCAAGGAGAAAGAAATATCACAGTGTTtgatg                                                                               <  1:776713/251‑1 (MQ=255)                                                                                                                                               aaGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGAGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCGAGAATTATTAATTATGCCTTTTATTAATCAAGGAGAAAGAAATATCACAGTG‑Ttgatga                                                                              <  1:483844/251‑1 (MQ=255)                                                                                                                                                 gagTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACAt                                                                                                                                                          >  1:78019/1‑174 (MQ=255)                                                                                                                                                 gagTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCGAGAATTATTAATTATGCCTTTTATTAATCAAGGagaaaga                                                                                                  >  1:742549/1‑230 (MQ=255)                                                                                                                                                  agTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCGAGAATTATTAATTATGCCTTTTATTAATCAAGGAGAAAGAAATATCACAGTGTTtgatga                                                                              <  1:102440/249‑1 (MQ=255)                                                                                                                                                        ggATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCGAGAattatt                                                                                                                                 <  1:504267/192‑1 (MQ=255)                                                                                                                                                        ggATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCGAGAATTATTAATTATGCCTTTTATTAATc                                                                                                             >  1:105814/1‑212 (MQ=255)                                                                                                                                                                                        ggTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCGAGAATTATTAATTATGCCTTTTATTAATCAAGGAGAAAGAAATATCACAg                                                                                        >  1:900137/1‑201 (MQ=255)                                                                                                                                                                                        ggTATGACTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCGAGAATTATTAATTATGCCTTTTATTAATCAAGGAGAAAGAAATATCACAGTGTTTGATGATGATGGCGAAAGTTTTGATTATCTCAAAGGTAAata                                          <  1:475715/247‑1 (MQ=255)                                                                                                                                                                                               cTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCGAGAATTATTAATTATGCCTTTTATTAATCAAGGAGAAAGAAATATCACAGTGTTTGATGATGATGGCGAAAGttt                                                               <  1:855142/219‑1 (MQ=255)                                                                                                                                                                                               cTTTTACACTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCGAGAATTATTAATTATGCCTTTTATTAATCAAGGAGAAAGAAATATCACAGTGTTTGATGATGATGGCGAAAGTTTTGATTATCTCAAAGGTAAATATTTAGAGCtt                                >  1:861545/1‑250 (MQ=255)                                                                                                                                                                                                    acacTCATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCGAGAATTATTAATTATGCCTTTTATTAATc                                                                                                             >  1:340541/1‑168 (MQ=255)                                                                                                                                                                                                         cATAAATGGTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCGAGAATTATTAATTATGCCTTTTATTAATCAAGGAGAAAGAAATATCACAGTGTTTGATGATGATGGCGAAAGTTTTGATTATCTCAAAGGTAAATATTTAGAGCTTCACATTCAt                       >  1:56724/1‑249 (MQ=255)                                                                                                                                                                                                                 gTTTAATGGTGGCGAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCGAGAattatt                                                                                                                                 >  1:558056/1‑135 (MQ=255)                                                                                                                                                                                                                       tggtggcgAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCGAGAATTATTAATTATGCCTTTTATTAATCAAGGAGAAAGAAATATCACAGTGTTTGATGATGATGGCGAAAGTTTTGATTATCTCAAAGGTAAATATTTAGAGCTTCACATTCATCTAAAATGCACGAAcc       <  1:129789/247‑1 (MQ=255)                                                                                                                                                                                                                             cgAAAAAATCACGGTAGATACGCCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCGAGAATTATTAATTATGCCTTTTATTAATCAAGGAGAAAGAAATATCACAGTGTTTGATGATGATGGCGAAAGTTTTGATTATCTCAAAGGTAAATATTTAGAGCTTCACATTCATCTAAAATGCACGAACCAAACa  <  1:728344/250‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                     TTTGGAAAGCACATCAAGATAACGAACCTATATTGCGTCCAACTTTCCTTGATCATGAACATGATAAAAACACATTTGCAGAAAATGATGATTATCTTCTTGGTGAAGATTTATTAGTCGCATCTGTGGTAGAACCAAATCAAAGAGTACGGGATGTTTATCTTCCACAAAATTTTGCAGGCTGGTATGACTTTTACACTCATAAATGGTTTAATGGCGGCGAAAAAATCACGGTAGATACACCATTAGAAAGGATCCCTTTATTTTTAAGAGCTGGAAGTGTCATTCCAATGGCGAGAAAAGGTCATAAAAATACATTATCTGATGATTATCGAGAATTATTAATTATGCCTTTTATTAATCAAGGAGAAAGAAATATCACAGTGTTTGATGATGATGGCGAAAGTTTTGATTATCTCAAAGGTAAATATTTAGAGCTTCACATTCATCTAAAATGCACGAACCAAACA  >  1/1273663‑1274132

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑