Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,263,816	T→G	S224A (TCG→GCG)	unknown →	D‑3‑phosphoglycerate dehydrogenase (EC 1.1.1.95)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,263,816	0	T	G	100.0%	207.5 / NA	77	S224A (TCG→GCG)	unknown	D‑3‑phosphoglycerate dehydrogenase (EC 1.1.1.95)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (34/43);  total (34/43)

ATCCGCCGTAGGTTCACACGAAGTGCGCGGCAAAAAACTGGGTATCGTAGGTTATGGTCATATCGGCTCCCAGCTGAGCATCATTGCCGAATCTTTGGGGATGGATGTGCATTTCTATGACATTGAAAACAAGCTCCCGCTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCTCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGATATTGACGCATTAGCAAAAGCTTTGGAAGAAGGCAAAGTGCGTGGTGCGGCAATTGACGTGTTCCCGAAAGAACCGGCTTCCATTAATGAAGAATTCGTTTCACCGTTACGCAAATTCGACAACGTGCTATTAACCCCGCATATCGGCGGTTCAACTGCCGAAGCGCAGGAAAA  >  1/1263572‑1264057                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  aTCCGCCGTAGGTTCACACGAAGTGCGCGGCAAAAAACTGGGTATCGTAGGTTATGGTCATATCGGCTCCCAGCTGAGCATCATTGCCGAATCTTTGGGGATGGATGTGCATTTCTATGACATTGAAAACAAGCTCCCGCTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAgc                                                                                                                                                                                                                                             <  1:41220/251‑1 (MQ=255)      ccgTAGGTTCACACGAAGTGCGCGGCAAAAAACTGGGTATCGTAGGTTATGGTCATATCGGCTCCCAGCTGAGCATCATTGCCGAATCTTTGGGGATGGATGTGCATTTCTATGACATTGAAAACAAGCTCCCGCTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCAt                                                                                                                                                                                                                                         <  1:670203/250‑1 (MQ=255)           ggTTCACACGAAGTGCGCGGCAAAAAACTGGGTATCGTAGGTTATGGTCATATCGGCTCCCAGCTGAGCATCATTGCCGAATCTTTGGGGATGGATGTGCATTTCTATGACATTGAAAACAAGCTCCCGCTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCa                                                                                                                                                                                                                                   >  1:39001/1‑251 (MQ=255)           ggTTCACACGAAGTGCGCGGCAAAAAACTGGGTATCGTAGGTTATGGTCATATCGGCTCCCAGCTGAGCATCATTGCCGAATCTTTGGGGATGGATGTGCATTTCTATGACATTGAAAACAAGCTCCCGCTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCa                                                                                                                                                                                                                                   >  1:824290/1‑251 (MQ=255)                   cGAAGTGCGCGGTAAAAAACTGGGTATCGTAGGTTATGGTCATATCGGCTCCCAGCTGAGCATCATTGCCGAATCTTTGGGGATGGATGTGCATTTCTATGACATTGAAAACAAGCTCCCGCTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGc                                                                                                                                                                                                                           <  1:294768/251‑1 (MQ=255)                   cGAAGTGCGCGGCAAAAAACTGGGTATCGTAGGTTATGGTCATATCGGCTCCCAGCCGAGCATCATTGCCGAATCTTTGGGGATGGATGTGCATTTCTATGACATTGAAAACAAGCTCCCGCTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGc                                                                                                                                                                                                                           <  1:341901/251‑1 (MQ=255)                              gCAAAAAACTGGGTATCGTAGGTTATGGTCATATCGGCTCCCAGCTGAGCATCATTGCCGAATCTTTGGGGATGGATGTGCATTTCTATGACATTGAAAACAAGCTCCCGCTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCAt                                                                                                                                                                                                                 <  1:704734/250‑1 (MQ=255)                               cAAAAAACTGGGTATCGTAGGTTATGGTCATATCGGCTCCCAGCTGAGCATCATTGCCGAATCTTTGGGGATGGATGTGCATTTCTATGACATTGAAAACAAGCTCCCGCTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCAtt                                                                                                                                                                                                                <  1:729061/250‑1 (MQ=255)                                        gggTATCGTAGGTTATGGTCATATCGGCTCCCAGCTGAGCATCATTGCCGAATCTTTGGGGATGGATGTGCATTTCTATGACATTGAAAACAAGCTCCCGCTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTa                                                                                                                                                                                                                               <  1:798594/226‑1 (MQ=255)                                        gggTATCGTAGGTTATGGTCATATCGGCTCCCAGCTGAGCATCATTGCCGAATCTTTGGGGATGGATGTGCATTTCTATGACATTGAAAACAAGCTCCCGCTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTttaatt                                                                                                                                                                                                          <  1:298510/247‑1 (MQ=255)                                        gggTATCGTAGGTTATGGTCATATCGGCTCCCAGCTGAGCATCATTGCCGAATCTTTGGGGATGGATGTGCATTTCTATGACATTGAAAACAAGCTCCCGCTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACATGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAacg                                                                                                                                                                                                      >  1:424024/1‑251 (MQ=255)                                          gTATCGTAGGTTATGGTCATATCGGCTCCCAGCTGAGCATCATTGCCGAATCTTTGGGGATGGATGTGCATTTCTATGACATTGAAAACAAGCTCCCGCTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAacgcg                                                                                                                                                                                                    >  1:511898/1‑251 (MQ=255)                                            aTCGTAGGTTATGGTCATATCGGCTCCCAGCTGAGCATCATTGCCGAATCTTTGGGGATGGATGTGCATTTCTATGACATTGAAAACAAGCTCCCGCTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAacgcgg                                                                                                                                                                                                   >  1:369542/1‑250 (MQ=255)                                                  ggTTATGGTCATATCGGCTCCCAGCTGAGCATCATTGCCGAATCTTTGGGGATGGATGTGCATTTCTATGACATTGAAAACAAGCTCCCGCTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCAt                                                                                                                                                                                                                 >  1:798507/1‑230 (MQ=255)                                                   gTTATGGTCATATCGGCTCCCAGCTGAGCATCATTGCCGAATCTTTGGGGATGGATGTGCATTTCTATGACATTGAAAACAAGCTCCCGCTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGc                                                                                                                                                                                                                           >  1:86808/1‑219 (MQ=255)                                                     tATGGTCATATCGGTTCCCAGCTGAGCATCATTGCGGAATCCTTGGGCATGGATGTGCATTTCTACGACATTGAAAACAAACTTCCGCTCGGTAATGCCAAACAAGTGCGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCTCTACACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGTGCGGAGCGCATTGCGCAGTTAAAACAGGATTCCATTTTAATTAACgcggcacgcggcac                                                                                                                                                                                         <  1:579984/251‑1 (MQ=255)                                                         gTCATATCGGCTCCCAGCTGAGCATCATTGCCGAATCTTTGGGGATGGATGTGCATTTCTATGACATTGAAAACAAGCTCCCGCTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTa                                                                                                                                                                                                                               >  1:872424/1‑209 (MQ=255)                                                         gTCATATCGGCTCCCAGCTGAGCATCATTGCCGAATCTTTGGGGATGGATGTGCATTTCTATGACATTGAAAACAAGCTCCCGCTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACggt                                                                                                                                                                                      <  1:646443/250‑1 (MQ=255)                                                             tatCGGCTCCCAGCTGAGCATCATTGCCGAATCTTTGGGGATGGATGTGCATTTCTATGACATTGAAAACAAGCTCCCGCTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGtggtgg                                                                                                                                                                                 >  1:812518/1‑251 (MQ=255)                                                             tatCGGCTCCCAGCTGAGCATCATTGCCGAATCTTTGGGGATGGATGTGCATTTCTATGACATTGAAAACAAGCTCCCGCTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGtggtgg                                                                                                                                                                                 >  1:550665/1‑251 (MQ=255)                                                              atCGGCTCCCAGCTGAGCATCATTGCCGAATCTTTGGGGATGGATGTGCATTTCTATGACATTGAAAACAAGCTCCCGCTCGGTAACGCCAAACAAGTACGT‑GCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGat                                                                                                                                                                               <  1:822003/251‑1 (MQ=255)                                                               tCGGCTCCCAGCTGAGCATCATTGCCGAATCTTTGGGGATGGATGTGCATTTCTATGACATTGAAAACAAGCTCCCGCTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGat                                                                                                                                                                               <  1:436782/251‑1 (MQ=255)                                                               tCGGCTCCCAGCTGAGCATCATTGCCGAATCTTTGGGGATGGATGTGCATTTCTATGACATTGAAAACAAGCTCCCGCTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGat                                                                                                                                                                               <  1:777142/251‑1 (MQ=255)                                                               tCGGCTCCCAGCTGAGCATCATTGCCGAATCTTTGGGGATGGATGTGCATTTCTATGACATTGAAAACAAGCTCCCGCTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGat                                                                                                                                                                               <  1:749412/251‑1 (MQ=255)                                                               tCGGCTCCCAGCTGAGCATCATTGCCGAATCTTTGGGGATGGATGTGCATTTCTATGACATTGAAAACAAGCTCCCGCTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGat                                                                                                                                                                               <  1:391691/251‑1 (MQ=255)                                                               tCGGCTCCCAGCTGAGCATCATTGCCGAATCTTTGGGGATGGATGTGCATTTCTATGACATTGAAAACAAGCTCCCGCTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGat                                                                                                                                                                               <  1:895572/251‑1 (MQ=255)                                                               tCGGCTCCCAGCTGAGCATCATTGCCGAATCTTTGGGGATGGATGTGCATTTCTATGACATTGAAAACAAGCTCCCGCTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGat                                                                                                                                                                               <  1:880641/251‑1 (MQ=255)                                                               tCGGCTCCCAGCTGAGCATCATTGCCGAATCTTTGGGGATGGATGTGCATTTCTATGACATTGAAAACAAGCTCCCGCTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGat                                                                                                                                                                               <  1:169495/251‑1 (MQ=255)                                                                  gCTCCCAGCTGAGCATCATTGCCGAATCTTTGGGGATGGATGTGCATTTCTATGACATTGAAAACAAGCTCCCGCTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGat                                                                                                                                                                               <  1:668615/248‑1 (MQ=255)                                                                  gCTCCCAGCTGAGCATCATTGCCGAATCTTTGGGGATGGATGTGCATTTCTATGACATTGAAAACAAGCTCCCGCTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGATAtt                                                                                                                                                                            >  1:249874/1‑251 (MQ=255)                                                                    tCCCAGCTGAGCATCATTGCCGAATCTTTGGGGATGGATGTGCATTTCTATGACATTGAAAACAAGCTCCCGCTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAacgc                                                                                                                                                                                                     >  1:440344/1‑224 (MQ=255)                                                                       cAGCTGAGCATCATTGCCGAATCTTTGGGGATGGATGTGCATTTCTATGACATTGAAAACAAGCTCCCGCTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGATATTGACg                                                                                                                                                                        >  1:424210/1‑250 (MQ=255)                                                                         gCTGAGCATCATTGCCGAATCTTTGGGGATGGATGTGCATTTCTATGACATTGAAAACAAGCTCCCGCTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGATATTGACGCAt                                                                                                                                                                     >  1:236578/1‑251 (MQ=255)                                                                            gAGCATCATTGCCGAATCTTTGGGGATGGATGTGCATTTCTATGACATTGAAAACAAGCTCCCGCTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGat                                                                                                                                                                               >  1:36600/1‑238 (MQ=255)                                                                                                        gatgTGCATTTCTATGACATTGAAAACAAGCTCCCGCTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCAttt                                                                                                                                                                                                               >  1:26284/1‑178 (MQ=255)                                                                                                        gatgTGCATTTCTATGACATTGAAAACAAGCTCCCGCTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGATATTGACGc                                                                                                                                                                       <  1:727518/218‑1 (MQ=255)                                                                                                        gatgTGCATTTCTATGACATTGAAAACAAGCTCCCGCTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGATATTGACGCATTAGCAAAAGCt                                                                                                                                                          >  1:83162/1‑231 (MQ=255)                                                                                                              cATTTCTATGACATTGAAAACAAGCTCCCGCTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGATATTGACGCATTAGCAAAAGCTTTGGAAGAAGGCAAAGTGCgtggtg                                                                                                                                 <  1:674450/250‑1 (MQ=255)                                                                                                                                 aCAAGCTCCCGCTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGATATTGACGCATTAGCAAAAGCTTTGGAAGAAGGCAAAg                                                                                                                                          <  1:126740/222‑1 (MQ=255)                                                                                                                                     gCTCCCGCTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGATATTGACGCATTAGCAAAAGCTTTGGAAGAAGGCAAAGTGCgt                                                                                                                                     >  1:160555/1‑223 (MQ=255)                                                                                                                                      cTCCCGCTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGat                                                                                                                                                                               >  1:590673/1‑180 (MQ=255)                                                                                                                                            cTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGATATTGACGCATTAGCAAAAGCTTTGga                                                                                                                                                     <  1:43157/200‑1 (MQ=255)                                                                                                                                            cTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGAGCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGATATTGACGCATTAGCAAAAGCTTTGGAAGAAGGCAAAGTg                                                                                                                                        <  1:466039/213‑1 (MQ=255)                                                                                                                                                   aCGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGATATTGACGCATTAGCAAAAGCTTTGGAAGAAGGCAAAGTGCGTGGTGCGGCAATTGACGTGTTCCCGAAAGAACCGGCTTCCAtt                                                                                           <  1:612718/251‑1 (MQ=255)                                                                                                                                                   aCGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGATATTGACGCATTAGCAAAAGCTTTGGAAGAAGGCAAAGTGCGTGGTGCGGCAATTGACGTGTTCCCGAAAGAACCGGCTTCCAtt                                                                                           <  1:270852/251‑1 (MQ=255)                                                                                                                                                    cGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAg                                                                                                                                                                                                                         <  1:630095/124‑1 (MQ=255)                                                                                                                                                    cGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGATATTGACGCATTAGCAAAAGCTTTGGAAGAAGGCAAAGTGCGTGGTGCGGCAATTGACGTGTTCCCGAAAGAACCGGCTTCCATTa                                                                                          >  1:852315/1‑251 (MQ=255)                                                                                                                                                      ccAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGATATTGACGCATTAGCAAAAGCTTTGGAAGAAGGCAAAGTGCGTGGTGCGGCAATTGACGTGTTCCCGAAAGAACCGGCTTCCATTAAt                                                                                        >  1:29030/1‑251 (MQ=255)                                                                                                                                                          aCAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGATATTGACGCATTAGCAAAAGCTTTGGAAGAAGGCAAAGTGCGTGGTGCGGCAATTGACGTGTTCCCGAAAGAACCGGCTTCCATTAATgaag                                                                                    <  1:825139/251‑1 (MQ=255)                                                                                                                                                             aGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGATATTGACGCATTAGCAAAAGCTTTGGAAGAAGGCAAAGTGCGTGGTGCGGCAATTGACGTGTTCCCGAAAGAACCGGCTTCCATTAATGAAGAAt                                                                                 >  1:355615/1‑251 (MQ=255)                                                                                                                                                             aGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGATATTGACGCATTAGCAAAAGCTTTGGAAGAAGGCAAAGTGCGTGGTGCGGCAATTGACGTGTTCCCGAAAGAACCGGCTTCCATTAATGAAGAAt                                                                                 >  1:783885/1‑251 (MQ=255)                                                                                                                                                             aGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGGGGTGGATATTGACGCATTAGCAAAAGCTTTGGAAGAAGGCAAAGTGCGTGGTGCGGCAATTGACGTGTTCCCGAAAGAACCGGCTTCCATTAATGAAGAAt                                                                                 >  1:422509/1‑251 (MQ=255)                                                                                                                                                               tACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGat                                                                                                                                                                               <  1:210896/155‑1 (MQ=255)                                                                                                                                                                   tAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGATATTGACGCATTAGCAAAAGCTTTGGAAGAAGGCAAAGTGCGTGGTGCGGCAATTGACGTGTTCCCGAAAGAACCGGCTTCCATTAATGAAGAATTCGttt                                                                           >  1:818577/1‑251 (MQ=255)                                                                                                                                                                     gCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGATATTGACGCATTAGCAAAAGCTTTGGAAGAAGGCAAAGTGCGTGGTGCGGCAATTGACGTGTTCCCGAAAGAACCGGCTTCCATTAATGAAGAATTCGtt                                                                            <  1:399765/248‑1 (MQ=255)                                                                                                                                                                             gaATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGat                                                                                                                                                                               >  1:112973/1‑141 (MQ=255)                                                                                                                                                                             gaATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGATATTGACGCATTAGCAAAAGCTTTGGAAGAAGGCAAAGTGCGTGGTGCGGCAATTGACGTGTTCCCGAAAGAACCGGCTTCCATTAATGAAGAATTCGTTTCACCGTTACg                                                                 >  1:753929/1‑251 (MQ=255)                                                                                                                                                                                        aTCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGATATTGACGCATTAGCAAAAGCTTTGGAAGAAGGCAAAGTGCGTGGTGCGGCAATTGACGTGTTCCCGAAAGAACCGGCTTCCATTAATGAAGAATTCGTTTCACCGTTACGCAAATTCGAc                                                       <  1:23099/250‑1 (MQ=255)                                                                                                                                                                                           cTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGATATTGACGCATTAGCAAAAGCTTTGGAAGAAGGCAAAGTGCGTGGTGCGGCAATTGACGTGTTCCCGAAAGAACCGGCTTCCATTAATGAAGAATTCGTTTCACCGTTACGCAAATTCGACAACg                                                   >  1:20302/1‑251 (MQ=255)                                                                                                                                                                                                  gtgatTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGATATTGACGCATTAGCAAAAGCTTTGGAAGAAGGCAAAGTGCGTGGTGCGGCAATTGACGTGTTCCCGAAAGAACCGGCTTCCATTAATGAAGAATTCGTTTCACCGTTACGCAAATTCGACAACGTGCTAtt                                            >  1:751091/1‑251 (MQ=255)                                                                                                                                                                                                   tgatTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGATATTGACGCATTAGCAAAAGCTTTGGAAGAAGGCAAAGTGCGTGGTGCGGCAATTGACGTGTTCCCGAAAGAACCGGCTTCCATTAATGAAGAATTCGTTTCACCGTTACGCAAATTCGACAACGTGCTAtt                                            <  1:838836/250‑1 (MQ=255)                                                                                                                                                                                                     atTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGATATTGACGCATTAGCAAAAGCTTTGGAAGAAGGCAAAGTGCGTGGTGCGGCAATTGACGTGTTCCCGAAAGAACCGGCTTCCATTAATGAAGAATTCGTTTCACCGTTACGCAAATTCGACAACGTGCTATTAAc                                         <  1:688217/251‑1 (MQ=255)                                                                                                                                                                                                     atTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGATATTGACGCATTAGCAAAAGCTTTGGAAGAAGGCAAAGTGCGTGGTGCGGCAATTGACGTGTTCCCGAAAGAACCGGCTTCCATTAATGAAGAATTCGTTTCACCGTTACGCAAATTCGACAACGTGCTATTAAc                                         <  1:627720/251‑1 (MQ=255)                                                                                                                                                                                                       ttCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGATATTGACGCATTAGCAAAAGCTTTGGAAGAAGGCAAAGTGCGTGGTGCGGCAATTGACGTGTTCCCGAAAGAACCGGCTTCCATTAATGAAGAATTCGTTTCACCGTTACGCAAATTCGACAACGTGCTATTAAccc                                       <  1:674545/251‑1 (MQ=255)                                                                                                                                                                                                       ttCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGATATTGACGCATTAGCAAAAGCTTTGGAAGAAGGCAAAGTGCGTGGTGCGGCAATTGACGTGTTCCCGAAAGAACCGGCTTCCATTAATGAAGAATTCGTTTCACCGTTACGCAAATTCGACAACGTGCTATTAAccc                                       <  1:530780/251‑1 (MQ=255)                                                                                                                                                                                                             gCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGATATTGACGCATTAGCAAAAGCTTTGGAAGAAGGCAAAGTGCGTGGTGCGGCAATTGACGTGTTCCCGAAAGa                                                                                                        <  1:768870/180‑1 (MQ=255)                                                                                                                                                                                                             gCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGATATTGACGCATTAGCAAAAGCTTTGGAAGAAGGCAAAGTGCGTGGTGCGGCAATTGACGTGTTCCCGAAAGAACCGGCTTCCATTAATGAAGAATTCGTTTCACCGTTACGCAAATTCGACAACGTGCTATTAAccc                                       <  1:478018/245‑1 (MQ=255)                                                                                                                                                                                                                cGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGATATTGACGCATTAGCAAAAGCTTTGGAAGAAGGCAAAGTGCGTGGTGCGGCAATTGACGTGTTCCCGAAAGAACCGGCTTCCATTAATGAAGAATTCGTTTCACCGTTACGCAAATTCGACAACGTGCTATTAACCCCGCATATcg                              >  1:164304/1‑251 (MQ=255)                                                                                                                                                                                                                         tttGCCTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGATATTGACGCATTAGCAAAAGCTTTGGAAGAAGGCAAAGTGCGTGGTGCGGCAATTGACGTGTTCCCGAAAGAACCGGCTTCCATTAATGAAGAATTCGTTTCACCGTTACGCAAATTCGACAACGTGCTATTAACCCCGCATATCGGCGGTTCaa                     >  1:602700/1‑251 (MQ=255)                                                                                                                                                                                                                             ccTTCCACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGATATTGACGCATTAGCAAAAGCTTTGGAAGAAGGCAAAGTGCGTGGTGCGGCAATTGACGTGTTCCCGAAAGAACCGGCTTCCATTAATGAAGAATTCGTTTCACCGTTACGCAAATTCGACAACGTGCTATTAACCCCGCATATcggcggatccaatg                  >  1:312907/1‑242 (MQ=255)                                                                                                                                                                                                                                  cACCCGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGATATTGACGCATTAGCAAAAGCTTTGGAAGAAGGCAAAGTGCGTGGTGCGGCAATTGACGTGTTCCCGAAAGAACCGGCTTCCATTAATGAAGAATTCGTTTCACCGTTACGCAAATTCGACAACGTGCTATTAACCCCGCATATCGGCGGTTCAACTGCCGAAg            <  1:765777/251‑1 (MQ=255)                                                                                                                                                                                                                                    cccGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGATATTGACGCATTAGCAAAAGCTTTGGAAGAAGGCAAAGTGCGTGGTGCGGCAATTGACGTGTTCCCGAAAGAACCGGCTTCCATTAATGAAGAATTCGTTTCACCGTTACGCAAATTCGACAACGTGCTATTAACCCCGCATATCGGCGGTTCAACTGc                 <  1:279375/244‑1 (MQ=255)                                                                                                                                                                                                                                    cccGTAATTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGATATTGACGCATTAGCAAAAGCTTTGGAAGAAGGCAAAGTGCGTGGTGCGGCAATTGACGTGTTCCCGAAAGAACCGGCTTCCATTAATGAAGAATTCGTTTCACCGTTACGCAAATTCGACAACGTGCTATTAACCCCGCATATCGGCGGTTCAACTGCCGAAgcg          >  1:322394/1‑251 (MQ=255)                                                                                                                                                                                                                                         aaTTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGATATTGACGCATTAGCAAAAGCTTTGGAAGAAGGCAAAGTGCGTGGTGCGGCAATTGACGTGTTCCCGAAAGAACCGGCTTCCATTAATGAAGAATTCGTTTCACCGTTACGCAAATTCGACAACGTGCTATTAAcc                                        <  1:901770/216‑1 (MQ=255)                                                                                                                                                                                                                                          aTTTAATCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGATATTGACGCATTAGCAAAAGCTTTGGAAGAAGGCAAAGTGCGTGGTGCGGCAATTGACGTGTTCCCGAAAGAACCGGCTTCCATTAATGAAGAATTCGTTTCACCGTTACGCAAATTCGACAACGTGCTATTAACCCCGCATATCGGCGGTTCAACTGCCGAAGCGCAGGaa    <  1:105816/251‑1 (MQ=255)                                                                                                                                                                                                                                              aaTCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGat                                                                                                                                                                               <  1:428401/76‑1 (MQ=255)                                                                                                                                                                                                                                              aaTCAGCGCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGATATTGACGCATTAGCAAAAGCTTTGGAAGAAGGCAAAGTGCGTGGTGCGGCAATTGACGTGTTCCCGAAAGAACCGGCTTCCATTAATGAAGAATTCGTTTCACCGTTACGCAAATTCGACAACGTGCTATTAACCCCGCATATCGGCGGtttaaacaccgcaacgcccccccc  >  1:638288/1‑227 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                  ATCCGCCGTAGGTTCACACGAAGTGCGCGGCAAAAAACTGGGTATCGTAGGTTATGGTCATATCGGCTCCCAGCTGAGCATCATTGCCGAATCTTTGGGGATGGATGTGCATTTCTATGACATTGAAAACAAGCTCCCGCTCGGTAACGCCAAACAAGTACGTAGCCTGGAGGAATTGCTCGGATCCTGTGATGTGATTTCCCTGCACGTGCCGGATTTGCCTTCCACCCGTAATTTAATCAGCTCGGAGCGCATCGCGCAGTTAAAGCAGGATTCCATTTTAATTAACGCGGCACGCGGCACGGTGGTGGATATTGACGCATTAGCAAAAGCTTTGGAAGAAGGCAAAGTGCGTGGTGCGGCAATTGACGTGTTCCCGAAAGAACCGGCTTCCATTAATGAAGAATTCGTTTCACCGTTACGCAAATTCGACAACGTGCTATTAACCCCGCATATCGGCGGTTCAACTGCCGAAGCGCAGGAAAA  >  1/1263572‑1264057

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑