Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,259,220	A→G	A197A (GCA→GCG)	unknown →	HflC protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,259,220	0	A	G	100.0%	171.6 / NA	62	A197A (GCA→GCG)	unknown	HflC protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (37/25);  total (37/25)

GCCAACTTATTACGTCGTAAAGTCAATGACCGTTTGCGTTCCGAAATCGGTTCCCGCACC‑ATTAAAGACATCGTTTCCGGTACACGAGGCGAACTCATGGTAGGCACGAAAAAAGCGCTCAACAGTGGGCAAGACAGCACCGCCGAACTGGGGATTGAAGTGCTCGACGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCAGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGTAGATCGTAAAGTCACCTTAATTATCGCCAATGCGGAAAAAACCGCACAGGAATTACGCGGTGACGGCGACGCGACCGCAGCCAAAATCTTTGCCGATGCCTTTGGTAAAGAGCCTGAATTTTACAGCTTCATTCGTAGCCTGAAAGCCTATGAAAGCAGCTTCGCCAACTCGGACAATTTGTTGA  >  1/1258975‑1259464                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     gcCAACTTATTACGTCGTAAAGTCAATGACCGTTTGCGTTCCGAAATCGGTTCCCGCACCAATTAAAGACATCGTTTCCGGTACACGAGGCGAACTCATGGTAGGCACGAAAAAAGCGCTCAACAGTGGGCAAGACAGCACCGCCGAACTGGGGATTGAAGTGCTCGACGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAg                                                                                                                                                                                                                                                  >  1:825167/1‑251 (MQ=255)      cTTATTACGTCGTAAAGTCAATGACCGTTTGCGTTCCGAAATCGGTTCCCGCACC‑ATTAAAGACATCGTTTCCGGTACACGAGGCGAACTCATGGTAGGCACGAAAAAAGCGCTCAACAGTGGGCAAGACAGCACCGCCGAACTGGGGATTGAAGTGCTCGACGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTg                                                                                                                                                                                                                                            >  1:660377/1‑251 (MQ=255)      cTTATTACGTCGTAAAGTCAATGACCGTTTGCGTTCCGAAATCGGTTCCCGCACC‑ATTAAAGACATCGTTTCCGGTACACGAGGCGAACTCATGGTAGGCACGAAAAAAGCGCTCAACAGTGGGCAAGACAGCACCGCCGAACTGGGGATTGAAGTGCTCGACGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTg                                                                                                                                                                                                                                            >  1:233130/1‑251 (MQ=255)       ttattaCGTCGTAAAGTCAATGACCGTTTGCGTTCCGAAATCGGTTCCCGCACC‑ATTAAAGACATCGTTTCCGGTACACGAGGCGAACTCATGGTAGGCACGAAAAAAGCGCTCAACAGTGGGCAAGACAGCACCGCCGAACTGGGGATTGAAGTGCTCGACGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGa                                                                                                                                                                                                                                           >  1:411347/1‑251 (MQ=255)         attaCGTCGTAAAGTCAATGACCGTTTGCGTTCCGAAGTCGGTTCCCGCACC‑ATTAAAGACATCGTTTCCGGTACACGAGGTGAACTCATGGTAGGCACGAAAAAAGCGCTCAACAGTGGGCAAGACAGCACCGCCGAACTGGGGATTGAAGTGCTCGACGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAac                                                                                                                                                                                                                                         <  1:456289/251‑1 (MQ=255)         attaCGTCGTAAAGTCAATGACCGTTTGCGTTCCGAAATCGGTTCCCGCACC‑ATTAAAGACATCGTTTCCGGTACACGAGGCGAACTCATGGTAGGCACGAAAAAAGCGCTCAACAGTGGGCAAGACAGCACCGCCGAACTGGGGATTGAAGTGCTCGACGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAac                                                                                                                                                                                                                                         <  1:248341/251‑1 (MQ=255)         attaCGTCGTAAAGTCAATGACCGTTTGCGTTCCGAAATCGGTTCCCGCACC‑ATTAAAGACATCGTTTCCGGTACACGAGGCGAACTCATGGTAGGCACGAAAAAAGCGCTCAACAGTGGGCAAGACAGCACCGCCGAACTGGGGATTGAAGTGCTCGACGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAac                                                                                                                                                                                                                                         <  1:419286/251‑1 (MQ=255)           taCGTCGTAAAGTCAATGACCGTTTGCGTTCCGAAATCGGTTCCCGCACC‑ATTAAAGACATCGTTTCCGGTACACGAGGCGAACTCATGGTAGGCACGAAAAAAGCGCTCAACAGTGGGCAAGACAGCACCGCCGAACTGGGGATTGAAGTGCTCGACGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAacac                                                                                                                                                                                                                                       <  1:664474/251‑1 (MQ=255)           taCGTCGTAAAGTCAATGACCGTTTGCGTTCCGAAATCGGTTCCCGCACC‑ATTAAAGACATCGTTTCCGGTACACGAGGCGAACTCATGGTAGGCACGAAAAAAGCGCTCAACAGTGGGCAAGACAGCACCGCCGAACTGGGGATTGAAGTGCTCGACGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAacac                                                                                                                                                                                                                                       <  1:642713/251‑1 (MQ=255)              gtcgtAAAGTCAATGACCGTTTGCGTTCCGAAATCGGTTCCCGCACC‑ATTAAAGACATCGTTTCCGGTACACGAGGCGAACTCATGGTAGGCACGAAAAAAGCGCTCAACAGTGGGCAAGACAGCACCGCCGAACTGGGGATTGAAGTGCTCGACGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACACCg                                                                                                                                                                                                                                     >  1:253311/1‑250 (MQ=255)               tcgtAAAGTCAATGACCGTTTGCGTTCCGAAATCGGTTCCCGCACC‑ATTAAAGACATCGTTTCCGGTACACGAGGCGAACTCATGGTAGGCACGAAAAAAGCGCTCAACAGTGGGCAAGACAGCACCGCCGAACTGGGGATTGAAGTGCTCGACGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACACCg                                                                                                                                                                                                                                     >  1:158137/1‑249 (MQ=255)                 gtAAAGTCAATGACCGTTTGCGTTCCGAAATCGGTTCCCGCACC‑ATTAAAGACATCGTTTCCGGTACACGAGGCGAACTCATGGTAGGCACGAAAAAAGCGCTCAACAGTGGGCAAGACAGCACCGCCGAACTGGGGATTGAAGTGCTCGACGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAac                                                                                                                                                                                                                                         <  1:224618/243‑1 (MQ=255)                       tCAATGACCGTTTGCGTTCCGAAATCGGTTCCCGCACC‑ATTAAAGACATCGTTTCCGGTACACGAGGCGAACTCATGGTAGGCACGAAAAAAGCGCTCAACAGTGGGCAAGACAGCACCGCCGAACTGGGGATTGAAGTGCTCGACGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACACCGCTCTCAAgg                                                                                                                                                                                                                            <  1:48065/250‑1 (MQ=255)                              ccGTTTGCGTTCCGAAATCGGTTCCCGCACC‑ATTAAAGACATCGTTTCCGGTACACGAGGCGAACTCATGGTAGGCACGAAAAAAGCGCTCAACAGTGGGCAAGACAGCACCGCCGAACTGGGGATTGAAGTTCTCGACGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGaaa                                                                                                                                                                                                                    <  1:124705/251‑1 (MQ=255)                                        tCCGAAATCGGTTCCCGCACC‑ATTAAAGACATCGTTTCCGGTACACGAGGCGAACTCATGGTAGGCACGAAAAAAGCGCTCAACAGTGGGCAAGACAGCACCGCCGAACTGGGGATTGAAGTGCTCGACGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCAtt                                                                                                                                                                                                          >  1:28691/1‑251 (MQ=255)                                                   ttCCCGCACC‑ATTAAAGACATCGTTTCCGGTACACGAGGCGAACTCATGGTAGGCACGAAAAAAGCGCTCAACAGTGGGCAAGACAGCACCGCCGAACTGGGGATTGAAGTGCTCGACGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCgg                                                                                                                                                                                               <  1:836634/251‑1 (MQ=255)                                                           cc‑ATTAAAGACATCGTTTCCGGTACACGAGGCGAACTCATGGTAGGCACGAAAAAAGCGCTCAACAGTGGGCAAGACAGCACCGCCGAACTGGGGATTGAAGTGCTCGACGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAac                                                                                                                                                                                                                                         >  1:532263/1‑201 (MQ=255)                                                           cc‑ATTAAAGACATCGTTTCCGGTACACGAGGCGAACTCATGGTAGGCACGAAAAAAGCGCTCAACAGTGGGCAAGACAGCACCGCCGAACTGGGGATTGAAGTGCTCGACGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACTCCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGTAGAt                                                                                                                                                                                       >  1:542939/1‑251 (MQ=255)                                                           cc‑ATTAAAGACATCGTTTCCGGTACACGAGGCGAACTCATGGTAGGCACGAAAAAAGCGCTCAACAGTGGGCAAGACAGCACCGCCGAACTGGGGATTGAAGTGCTCGACGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGTAg                                                                                                                                                                                         >  1:113666/1‑249 (MQ=255)                                                           cc‑ATTAAAGACATCGTTTCCGGTACACGAGGCGAACTCATGGTAGGCACGAAAAAAGCGCTCAACAGTGGGCAAGACAGCACCGCCGAACTGGGGATTGAAGTGCTCGACGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGTAGAt                                                                                                                                                                                       <  1:481126/251‑1 (MQ=255)                                                           cc‑ATTAAAGACATCGTTTCCGGTACACGAGGCGAACTCATGGTAGGCACGAAAAAAGCGCTCAACAGTGGGCAAGACAGCACCGCCGAACTGGGGATTGAAGTGCTCGACGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGTAGAt                                                                                                                                                                                       >  1:874875/1‑251 (MQ=255)                                                                                         ggCGAACTCATGGTAGGCACGAAAAAAGCGCTCAACAGTGGGCAAGACAGCACCGCCGAACTGGGGATTGAAGTGCTCGCCGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGCACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGTAGAt                                                                                                                                                                                       >  1:455224/1‑222 (MQ=255)                                                                                             aaCTCATGGTAGGCACGAAAAAAGCGCTCAACAGTGGGCAAGACAGCACCGCCGAACTGGGGATTGAAGTGCTCGACGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGTAGATCGTAAAGTCACCTTAATTa                                                                                                                                                                    >  1:670817/1‑237 (MQ=255)                                                                                              aCTCATGGTAGGCACGAAAAAAGCGCTCAACAGTGGGCAAGACAGCACCGCCGAACTGGGGATTGAAGTGCTCGACGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGTAGATCGTAAAGTCACCTTAATTATCGCCAATGCGGaaa                                                                                                                                                     <  1:817503/251‑1 (MQ=255)                                                                                                 cATGGTAGGCACGAAAAAAGCGCTCAACAGTGGGCAAGACAGCACCGCCGAACTGGGGATTGAAGTGCTCGACGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGTAGATCGTAAAGTCACCTTAATTATCGCCAATGCGGaaaaaa                                                                                                                                                  >  1:172845/1‑251 (MQ=255)                                                                                                                      gcTCAACAGTGGGCAAGACAGCACCGCCGAACTGGGGATTGAAGTGCTCGACGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGt                                                                                                                                                                                           >  1:184620/1‑189 (MQ=255)                                                                                                                      gcTCAACAGTGGGCAAGACAGCACCGCCGAACTGGGGATTGAAGTGCTCGACGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGTAGATCGTAAAGTCACCTTAATTATCGCCAATGCGGAAAAAACCGCACAGGAATTACGCGGTg                                                                                                                             >  1:232706/1‑251 (MQ=255)                                                                                                                         cAACAGTGGGCAAGACAGCACCGCCGAACTGGGGATTGAAGTGCTCGACGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGTAGATCGTAAAGTCACCTTAATTATCGCCAATGCGGAAAAAACCGCACAGGAATTACGCGGTGACg                                                                                                                          >  1:162244/1‑251 (MQ=255)                                                                                                                           aCAGTGGGCAAGACAGCACCGCCGAACTGGGGATTGAAGTGCTCGACGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGTAGAt                                                                                                                                                                                       <  1:388820/188‑1 (MQ=255)                                                                                                                           aCAGTGGGCAAGACAGCACCGCCGAACTGGGGATTGAAGTGCTCGACGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGTAGATCGTAAAGTCACCTTAATTATCGCCAATGCGGAAAAAACCGCACAGGAATTACGCGGTGACgg                                                                                                                         >  1:35102/1‑250 (MQ=255)                                                                                                                                  gCAAGACAGCACCGCCGAACTGGGGATTGAAGTGCTCGACGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGTAGATCGTAAAg                                                                                                                                                                                >  1:682366/1‑188 (MQ=255)                                                                                                                                     aGACAGCACCGCCGAACTGGGGATTGAAGTGCTCGACGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGTAGATCGTAAAGTCACCTTAATTATCGCCAATGCGGAAAAAACCGCACAGGAATTACGCGGTGACGGCGACGCGACCg                                                                                                              >  1:310521/1‑251 (MQ=255)                                                                                                                                           cACCGCCGAACTGGGGATTGAAGTGCTCGACGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGTAGATCGTAAAGTCACCTTAATTATCGCCAATGCGGAAAAAACCGCACAGGAATTACGCGGTGACGGCGACGCGACCGCAg                                                                                                           <  1:804725/248‑1 (MQ=255)                                                                                                                                                         ggATTGAAGTGCTCGACGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGt                                                                                                                                                                                           <  1:500236/154‑1 (MQ=255)                                                                                                                                                         ggATTGAAGTGCTCGACGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGTAGATCGTAAAGTCACCTTAATTATCGCCAATGCGGAAAAAACCGCACAGGAATTACGCGGTGACGgcgacg                                                                                                                    <  1:243313/225‑1 (MQ=255)                                                                                                                                                            ttGAAGTGCTCGACGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGTAGATCGTAAAGTCACCTTAATTATCGCCAATGCGGaaa                                                                                                                                                     >  1:72879/1‑189 (MQ=255)                                                                                                                                                                  tGCTCGACGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGTAGATCGTAAAGTCACCTTAATTATCGCCAATGCGGAAAAAACCGCACAGGAATTACGCGGTGACGGCGACGCGACCGCAGCCAAAATCTTTGCCGATGCCTTTGGt                                                                                 <  1:671285/251‑1 (MQ=255)                                                                                                                                                                        aCGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCg                                                                                                                                                                                                >  1:543269/1‑134 (MQ=255)                                                                                                                                                                        aCGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGTAGATCGTAAAGTCACCTTAATTATCGCCAATGCGGAAAAAACCGCACAGGAATTACGCGGTGACGGCGACGCGACCGCAGCCAAAATCTTTGCCGATGCCTTTGGTAAagag                                                                           >  1:875190/1‑251 (MQ=255)                                                                                                                                                                        aCGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGTAGATCGTAAAGTCACCTTAATTATCGCCAATGCGGAAAAAACCGCACAGGAATTACGCGGTGACGGCGACGCGACCGCAGCCAAAATCTTTGCCGATGCCTTTGGTAAagag                                                                           <  1:522699/251‑1 (MQ=255)                                                                                                                                                                         cGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGTAGATCGTAAAGTCACCTTAATTATCGCCAATGCGGAAAAAACCGCACAGGAATTACGCGGTGACGGCGACGCGACCGCAGCCAAAATCTTTGCCGATGCCTTTGGTAAAGAGc                                                                          <  1:40451/251‑1 (MQ=255)                                                                                                                                                                               gATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAacac                                                                                                                                                                                                                                       >  1:703958/1‑88 (MQ=255)                                                                                                                                                                               gATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAac                                                                                                                                                                                                                                         <  1:166885/86‑1 (MQ=255)                                                                                                                                                                               gATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGTAGATCGTAAAGTCACCTTAATTATCGCCAATGCGGAAAAAACCGCACAGGAATTACGCGGTGACGGCGACGCGACCGCAGCCAAAATCTTTGCCGATGCCTTTGGTAAAGAGCCTGAAt                                                                    >  1:596476/1‑251 (MQ=255)                                                                                                                                                                                 ttAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGTAGATCGTAAAGTCACCTTAATTATCGCCAATGCGGAAAAAACCGCACAGGAATTACGCGGTGACGGCGACGCGACCGCAGCCAAAATCTTTGCCGATGCCTTTGGTAAAGAGCCTGAAttt                                                                  <  1:311625/251‑1 (MQ=255)                                                                                                                                                                                                 gCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGTAGATCGTAAAGTCACCTTAATTATCGCCAATGCGGAAAAAACCGCACAGGAATTACGCGGTGACGGCGACGCGACCGCAGCCAAAATCTTTGCCGATGCCTTTGGTAAAGAGCCTGAATTTTACAGCTTCATTCGTa                                                  >  1:106498/1‑251 (MQ=255)                                                                                                                                                                                                    ggATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGTAGATCGTAAAGTCACCTTAATTATCGCCAATGCGGAAAAAACCGCACAGGAATTACGCGGTGACGGCGACGCGACCGCAGCCAAAATCTTTGCCGATGCCTTTGGTAAAGAGCCTGAATTTTACAGCTTCATTCGTAGc                                                >  1:146173/1‑250 (MQ=255)                                                                                                                                                                                                     gATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGTAGATCGTAAAGTCACCTTAATTATCGCCAATGCGGAAAAAACCGCACAGGAATTACGCGGTGACGGCGACGCGACCGCAGCCAAAATCTTTGCCGATGCCTTTGGTAAAGAGCCTGAATTTTACAGCTTCATTCGTAGc                                                >  1:866976/1‑249 (MQ=255)                                                                                                                                                                                                     gATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGTAGATCGTAAAGTCACCTTAATTATCGCCAATGCGGAAAAAACCGCACAGGAATTACGCGGTGACGGCGACGCGACCGCAGCCAAAATCTTTGCCGATGCCTTTGGTAAAGAGCCTGAATTTTACAGCTTCATTCGTAGc                                                >  1:143166/1‑249 (MQ=255)                                                                                                                                                                                                                  cctccATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGTAGATCGTAAAGTCACCTTAATTATCGCCAATGCGGAAAAAACCGCACAGGAATTACGCGGTGACGGCGACGCGACCGCAGCCAAAATCTTTGCCGATGCCTTTGGTAAAGAGCCTGAATTTTACAGCTTCATTCGTAGCCTGAAAGCCTATGaa                                 <  1:174130/251‑1 (MQ=255)                                                                                                                                                                                                                     ccATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGTAGATCGTAAAGTCACCTTAATTATCGCCAATGCGGAAAAAAc                                                                                                                                                 >  1:199366/1‑136 (MQ=255)                                                                                                                                                                                                                     ccATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGTAGATCGTAAAGTCACCTTAATTATCGCCAATGCGGAAAAAACCGCACAGGAATTACGCGGTGACGGCGACGCGACCGCAGCCAAAATCTTTGCCGATGCCTTTGGTAAAGAGCCTGAATTTTACAGCTTCATTCGTAGCCTGAAAGCCTATGAAagc                              <  1:313079/251‑1 (MQ=255)                                                                                                                                                                                                                     ccATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGTAGATCGTAAAGTCACCTTAATTATCGCCAATGCGGAAAAAACCGCACAGGAATTACGCGGTGACGGCGACGCGACCGCAGCCAAAATCTTTGCCGATGCCTTTGGTAAAGAGCCTGAATTTTACAGCTTCATTCGTAGCCTGAAAGCCTATGAAagc                              <  1:308135/251‑1 (MQ=255)                                                                                                                                                                                                                     ccATTTACCAGCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGTAGATCGTAAAGTCACCTTAATTATCGCCAATGCGGAAAAAACCGCACAGGAATTACGCGGTGACGGCGACGCGACCGCAGCCAAAATCTTTGCCGATGCCTTTGGTAAAGAGCCTGAATTTTACAGCTTCATTCGTAGCCTGAAAGCCTATGAAag                               >  1:462000/1‑250 (MQ=255)                                                                                                                                                                                                                               gCGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGTAGATCGTAAAGTCACCTTAATTATCGCCAATGCGGAAAAAACCGCACAGGAATTACGCGGTGACGGCGACGCGACCGCAGCCAAAATCTTTGCCGATGCCTTTGGTAAAGAGCCTGAATTTTACAGCTTCATTCGTAGCCTGAAAGCCTATGAAAGCAGCTTCGccg                    >  1:807983/1‑250 (MQ=255)                                                                                                                                                                                                                                cGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGTAGATCGTAAAGTc                                                                                                                                                                              <  1:700541/96‑1 (MQ=255)                                                                                                                                                                                                                                cGTATGCGCGCCGAACGGGATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGTAGATCGTAAAGTCACCTTAATTATCGCCAATGCGGAAAAAACCGCACAGGAATTACGCGGTGACGGCGACGCGACCGCAGCCAAAATCTTTGCCGATGCCTTTGGTAAAGAGCCTGAATTTTACAGCTTCATTCGTAGCCTGAAAGCCTATGAAAGCAGCTTCGccga                   >  1:24797/1‑249 (MQ=255)                                                                                                                                                                                                                                        cgcCGAACGGGATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGTAGATCGTAAAGTCACCTTAATTATCGCCAATGCGGAAAAAACCGCACAGGAATTACGCGGTGACGGCGACGCGACCGCAGCCAAAATCTTTGCCGATGCCTTTGGTAAAGAGCCTGAATTTTACAGCTTCATTCGTAGCCTGAAAGCCTATGAAAGCAGCTTCGCCGACTCGGACa           >  1:326944/1‑251 (MQ=255)                                                                                                                                                                                                                                            gAACGGGATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGTAGATCGTAAAGTCACCTTAATTATCGCCAATGCGGAAAAAACCGCACAGGAATTACGCGGTGACGGCGACGCGACCGCAGCCAAAATCTTTGCCGATGCCTTTGGTAAAGAGCCTGAATTTTACAGCTTCATTCGTAGCCTGAAAGCCTATGAAAGCAGCTTCGCCGACTCGGACAAttt       >  1:26109/1‑251 (MQ=255)                                                                                                                                                                                                                                                 ggATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGTAGATCGTAAAGTCACCTTAATTATCGCCAATGCGGAAAAAACCGCACAGGAATTACGCGGTGACGGCGACGCGACCGCAGCCAAAATCTTTGCCGATGCCTTTGGTAAAGAGCCTGAAt                                                                    <  1:47302/185‑1 (MQ=255)                                                                                                                                                                                                                                                 ggATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGTAGATCGTAAAGTCACCTTAATTATCGCCAATGCGGAAAAAACCGCACAGGAATTACGCGGTGACGGCGACGCGACCGCAGCCAAAATCTTTGCCGATGCCTTTGGTAAAGAGCCTGAATTTTACAGCTTCATTCGTAGCCTGAAAGCCTATGAAAGCAGCTTCGCCGACTCGGACAATTTGTTGa  >  1:392646/1‑251 (MQ=255)                                                                                                                                                                                                                                                 ggATGCGGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGTAGATCGTAAAGTCACCTTAATTATCGCCAATGCGGAAAAAACCGCACAGGAATTACGCGGTGACGGCGACGCGACCGCAGCCAAAATCTTTGCCGATGCCTTTGGTAAAGAGCCTGAATTTTACAGCTTCATTCGTAGCCTGAAAGCCTATGAAAGCAGCTTCGCCGACTCGGACAATTTGTTGa  >  1:890475/1‑251 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                                     GCCAACTTATTACGTCGTAAAGTCAATGACCGTTTGCGTTCCGAAATCGGTTCCCGCACC‑ATTAAAGACATCGTTTCCGGTACACGAGGCGAACTCATGGTAGGCACGAAAAAAGCGCTCAACAGTGGGCAAGACAGCACCGCCGAACTGGGGATTGAAGTGCTCGACGTACGGATTAAACAAATTAACTTGCCGGATGAAGTGTCTTCCTCCATTTACCAGCGTATGCGCGCCGAACGGGATGCAGTAGCCCGTGAACACCGCTCTCAAGGTAAAGAAAAAGCGGCATTTATTCAGGCGGATGTAGATCGTAAAGTCACCTTAATTATCGCCAATGCGGAAAAAACCGCACAGGAATTACGCGGTGACGGCGACGCGACCGCAGCCAAAATCTTTGCCGATGCCTTTGGTAAAGAGCCTGAATTTTACAGCTTCATTCGTAGCCTGAAAGCCTATGAAAGCAGCTTCGCCAACTCGGACAATTTGTTGA  >  1/1258975‑1259464

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑