Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,257,604	T→C	A76A (GCT→GCC)	unknown →	HflK protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,257,604	0	T	C	100.0%	187.2 / NA	66	A76A (GCT→GCC)	unknown	HflK protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/29);  total (37/29)

CAAACGGAGAAAGCAATGTCGTTAAACGGTTCAGATCAAGATCCTTGGGGCAAACCAACGCAAAACGGACAATCGTCAGGAAACTCCTCTTCCGATAAAAACGAAGGTCAGTCCAATTGGGATCGTTCTTCCAATTCACAAAAAAAGAACGAGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCTAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATACCATCAAAGAAGCGGAGCGCGGCGTGGTGTTGCGTTTGGGGCAGTTCCATTCCATCGAACAACCGGGTTTAAACTGGAAACCGACCTTCATTGATCGCGTCATTCCGGTCAATGTGGAACGCGTACAGGAATTAAAAACTCAAGGCTCCATGCTGACACAAGA  >  1/1257362‑1257849                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      cAAACGGAGAAAGCAATGTCGTTAAACGGTTCAGATCAAGATCCTTGGGGCAAACCAACGCAAAACGGACAATCGTCAGGAAACTCCTCTTCCGATAAAAACGAAGGTCAGTCCAATTGGGATCGTTCTTCCAATTCACAAAAAAAGAACGAGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGc                                                                                                                                                                                                                                                >  1:647669/1‑250 (MQ=255)     cGGAGAAAGCAATGTCGTTAAACGGTTCAGATCAAGATCCTTGGGGCAAACCAACGCAAAACGGACAATCGTCAGGAAACTCCTCTTCCGATAAAAACGAAGGTCAGTCCAATTGGGATCGTTCTTCCAATTCACAAAAAAAGAACGAGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTc                                                                                                                                                                                                                                            >  1:631967/1‑250 (MQ=255)                   tCGTTAAACGGTTCAGATCAAGATCCTTGGGGCAAACCAACGCAAAACGGACAATCGTCAGGAAACTCCTCTTCCGATAAAAACGAAGGTCAGTCCAATTGGGATCGTTCTTCCAATTCACAAAAAAAGAACGAGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAatt                                                                                                                                                                                                                             >  1:615063/1‑251 (MQ=255)                   tCGTTAAACGGTTCAGATCAAGATCCTTGGGGCAAACCAACGCAAAACGGACAATCGTCAGGAAACTCCTCTTCCGATAAAAACGAAGGTCAGTCCAATTGGGATCGTTCTTCCAATTCACAAAAAAAGAACGAGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAatt                                                                                                                                                                                                                             <  1:323658/251‑1 (MQ=255)                    cGTTAAACGGTTCAGATCAAGATCCTTGGGGCAAACCAACGCAAAACGGACAATCGTCAGGAAACTCCTCTTCCGATAAAAACGAAGGTCAGTCCAATTGGGATCGTTCTTCCAATTCACAAAAAAAGAACGAGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAatta                                                                                                                                                                                                                            <  1:567114/251‑1 (MQ=255)                    cGTTAAACGGTTCAGATCAAGATCCTTGGGGCAAACCAACGCAAAACGGACAATCGTCAGGAAACTCCTCTTCCGATAAAAACGAAGGTCAGTCCAATTGGGATCGTTCTTCCAATTCACAAAAAAAGAACGAGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCCTCCGGCTTAGGTAAatta                                                                                                                                                                                                                            <  1:96883/251‑1 (MQ=255)                        aaaCGGTTCAGATCAAGATCCTTGGGGCAAACCAACGCAAAACGGACAATCGTCAGGAAACTCCTCTTCCGATAAAAACGAAGGTCAGTCCAATTGGGATCGTTCTTCCAATTCACAAAAAAAGAACGAGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGc                                                                                                                                                                                                                        <  1:117107/251‑1 (MQ=255)                             gTTCAGATCAAGATCCTTGGGGCAAACCAACGCAAAACGGACAATCGTCAGGAAACTCCTCTTCCGATAAAAACGAAGGTCAGTCCAATTGGGATCGTTCTTCCAATTCACAAAAAAAGAACGAGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAattat                                                                                                                                                                                                                           >  1:2873/1‑243 (MQ=255)                             gTTCAGATCAAGATCCTTGGGGCAAACCAACGCAAAACGGACAATCGTCAGGAAACTCCTCTTCCGATAAAAACGAAGGTCAGTCCAATTGGGATCGTTCTTCCAATTCACAAAAAAAGAACGAGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAatta                                                                                                                                                                                                                            <  1:565527/242‑1 (MQ=255)                             gTTCAGATCAAGATCCTTGGGGCAAACCAACGCAAAACGGACAATCGTCAGGAAACTCCTCTTCCGATAAAAACGAAGGTCAGTCCAATTGGGATCGTTCTTCCAATTCACAAAAAAAGAACGAGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGAtt                                                                                                                                                                                                                   >  1:139898/1‑251 (MQ=255)                                  gATCAAGATCCTTGGGGCAAACCAACGCAAAACGGACAATCGTCAGGAAACTCCTCTTCCGATAAAAACGAAGGTCAGTCCAATTGGGATCGTTCTTCCAATTCACAAAAAAAGAACGAGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTa                                                                                                                                                                                                                                 >  1:372086/1‑232 (MQ=255)                                  gATCAAGATCCTTGGGGCAAACCAACGCAAAACGGACAATCGTCAGGAAACTCCTCTTCCGATAAAAACGAAGGTCAGTCCAATTGGGATCGTTCTTCCAATTCACAAAAAAAGAACGAGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCAt                                                                                                                                                                                                              >  1:754521/1‑251 (MQ=255)                                        gATCCTTGGGGCAAACCAACGCAAAACGGACAATCGTCAGGAAACTCCTCTTCCGATAAAAACGAAGGTCAGTCCAATTGGGATCGTTCTTCCAATTCACAAAAAAAGAACGAGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCgg                                                                                                                                                                                                         >  1:24632/1‑250 (MQ=255)                                         aTCCTTGGGGCAAACCAACGCAAAACGGACAATCGTCAGGAAACTCCTCTTCCGATAAAAACGAAGGTCAGTCCAATTGGGATCGTTCTTCCAATTCACAAAAAAAGAACGAGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCgg                                                                                                                                                                                                         <  1:795174/249‑1 (MQ=255)                                          tCCTTGGGGCAAACCAACGCAAAACGGACAATCGTCAGGAAACTCCTCTTCCGATAAAAACGAAGGTCAGTCCAATTGGGATCGTTCTTCCAATTCACAAAAAAAGAACGAGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAg                                                                                                                                                                                                      >  1:12485/1‑251 (MQ=255)                                          tCCTTGGGGCAAACCAACGCAAAACGGACAATCGTCAGGAAACTCCTCTTCCGATAAAAACGAAGGTCAGTCCAATTGGGATCGTTCTTCCAATTCACAAAAAAAGAACGAGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAg                                                                                                                                                                                                      >  1:550208/1‑251 (MQ=255)                                             ttGGGGCAAACCAACGCAAAACGGACAATCGTCAGGAAACTCCTCTTCCGATAAAAACGAAGGTCAGTCCAATTGGGATCGTTCTTCCAATTCACAAAAAAAGAACGAGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCg                                                                                                                                                                                                   <  1:284824/251‑1 (MQ=255)                                                     aaCCAACGCAAAACGGACAATCGTCAGGAAACTCCTCTTCCGATAAAAACGAAGGTCAGTCCAATTGGGATCGTTCTTCCAATTCACAAAAAAAGAACGAGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTa                                                                                                                                                                                           >  1:337042/1‑251 (MQ=255)                                                       ccAACGCAAAACGGACAATCGTCAGGAAACTCCTCTTCCGATAAAAACGAAGGTCAGTCCAATTGGGATCGTTCTTCCAATTCACAAAAAAAGAACGAGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATCCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATg                                                                                                                                                                                         <  1:670289/251‑1 (MQ=255)                                                        cAACGCAAAACGGACAATCGTCAGGAAACTCCTCTTCCGATAAAAACGAAGGTCAGTCCAATTGGGATCGTTCTTCCAATTCACAAAAAAAGAACGAGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATgg                                                                                                                                                                                        <  1:678192/251‑1 (MQ=255)                                                                            tCAGGAAACTCCTCTTCCGATAAAAACGAAGGTCAGTCCAATTGGGATCGTTCTTCCAATTCACAAAAAAAGAACGAGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATAc                                                                                                                                                                    <  1:780754/251‑1 (MQ=255)                                                                                    ctcctcTTCCGATAAAAACGAAGGTCAGTCCAATTGGGATCGTTCTTCCAATTCACAAAAAAAGAACGAGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATACCATCAaag                                                                                                                                                            >  1:521999/1‑251 (MQ=255)                                                                                                     aCGAAGGTCAGTCCAATTGGGATCGTTCTTCCAATTCACAAAAAAAGAACGAGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATAc                                                                                                                                                                    <  1:722299/226‑1 (MQ=255)                                                                                                          ggTCAGTCCAATTGGGATCGTTCTTCCAATTCACAAAAAAAGAACGAGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATACCATCAAAGAAGCGGAGCGCGGCGTGGTGtt                                                                                                                                      >  1:291154/1‑251 (MQ=255)                                                                                                           gTCAGTCCAATTGGGATCGTTCTTCCAATTCACAAAAAAAGAACGAGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGTGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATACCATCAAAGAAGCGGAGCGCGGCGTGgtgt                                                                                                                                       <  1:404178/249‑1 (MQ=255)                                                                                                               gTCCAATTGGGATCGTTCTTCCAATTCACAAAAAAAGAACGAGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTAt                                                                                                                                                                                          >  1:380360/1‑194 (MQ=255)                                                                                                               gTCCAATTGGGATCGTTCTTCCAATTCACAAAAAAAGAACGAGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATACCATCAAAGAAGCGGAGCGCGGCgtggt                                                                                                                                         >  1:652894/1‑243 (MQ=255)                                                                                                               gTCCAATTGGGATCGTTCTTCCAATTCACAAAAAAAGAACGAGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATACCATCAAAGAAGCGGAGCGCGGCgtgg                                                                                                                                          >  1:422752/1‑242 (MQ=255)                                                                                                               gTCCAATTGGGATCGTTCTTCCAATTCACAAAAAAAGAACGAGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATACCATCAAAGAAGCGGAGCGCGGCGTGGTGTTGCGtt                                                                                                                                 >  1:273039/1‑251 (MQ=255)                                                                                                               gTCCAATTGGGATCGTTCTTCCAATTCACAAAAAAAGAACGAGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATACCATCAAAGAAGCGGAGCGCGGCGTGGTGTTGCGtt                                                                                                                                 >  1:26962/1‑251 (MQ=255)                                                                                                               gTCCAATTGGGATCGTTCTTCCAATTCACAAAAAAAGAACGAGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATACCATCAAAGAAGCGGAGCGCGGCGTGGTGTTGCGt                                                                                                                                  <  1:274430/250‑1 (MQ=255)                                                                                                               gTCCAATTGGGATCGTTCTTCCAATTCACAAAAAAAGAACGAGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTACCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATACCATCAAAGAAGCGGAGCGCGGCGTGGTGTTGCGtt                                                                                                                                 >  1:443615/1‑251 (MQ=255)                                                                                                                tCCAATTGGGATCGTTCTTCCAATTCACAAAAAAAGAACGAGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATACCATCAAAGAAGCGGAGCGCGGCGTGGTGTTGCGt                                                                                                                                  <  1:38570/249‑1 (MQ=255)                                                                                                                   aaTTGGGATCGTTCTTCCAATTCACAAAAAAAGAACGAGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATACCATCAAAGAAGCGGAGCGCGGCGTGGTGTTGCGTTTggg                                                                                                                             >  1:734666/1‑251 (MQ=255)                                                                                                                     ttGGGATCGTTCTTCCAATTCACAAAAAAAGAACGAGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATACCATCAAAGAAGCGGAGCGCGGCGTGGTGTTGCGTTTgggg                                                                                                                            >  1:771698/1‑250 (MQ=255)                                                                                                                              ttcttcCAATTCACAAAAAAAGAACGAGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGc                                                                                                                                                                                                                        >  1:91934/1‑149 (MQ=255)                                                                                                                               tcttcCAATTCACAAAAAAAGAACGAGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATACCATCAAAGAAGCGGAGCGCGGCGTGGTGTTGCGTTTGGGGCAGttccattc                                                                                                                 <  1:659270/251‑1 (MQ=255)                                                                                                                                 ttcCAATTCACAAAAAAAGAACGAGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATACCATCAAAGAAGCGGAGCGCGGCGTGGTGTTGCg                                                                                                                                   >  1:277981/1‑231 (MQ=255)                                                                                                                                                     aCGAGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATACCATCAAAGAAGCGGAGCGCGGCGTGGTGTTGCGTTTGGGGCAGTTCCATTCCATCGAACAACCGGGTTTAAAc                                                                                           <  1:780536/251‑1 (MQ=255)                                                                                                                                                       gAGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATACCATCAAAGAAGCGGAGCGCGGCGTGGTGTTGCGTTTGGGGCAGTTCCATTCCATCGAACAACCGGGTTTaaa                                                                                            >  1:147964/1‑248 (MQ=255)                                                                                                                                                        aGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATACCATCAAAGAAGCGGAg                                                                                                                                                    <  1:639900/191‑1 (MQ=255)                                                                                                                                                        aGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATACCATCAAAGAAGCGGAGCGCGGCGTGGTGTTGCGTTTGGGGCAGTTCCATTCCATCGAACAACCGGGTTTAAACTgg                                                                                        >  1:885629/1‑251 (MQ=255)                                                                                                                                                        aGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATACCATCAAAGAAGCGGAGCGCGGCGTGGTGTTGCGTTTGGGGCAGTTCCATTCCATCGAACAACCGGGTTTAAACTgg                                                                                        >  1:765660/1‑251 (MQ=255)                                                                                                                                                            aTCACCGCCCGATTTAGAAGAAGTATTCAAGAAGTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAAGTCCTATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCATGATTTTATGGGGCGCGAGCGGTTTTTTTATCATCAAAGAAGCGGAGTGCGGCGTGGTGTTGCGTTTGGGGCAGttccattcc                                                                                                                <  1:882614/223‑1 (MQ=255)                                                                                                                                                                       aTTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATAc                                                                                                                                                                    <  1:612198/160‑1 (MQ=255)                                                                                                                                                                       aTTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATACCATCAaaga                                                                                                                                                           <  1:872124/169‑1 (MQ=255)                                                                                                                                                                        tttAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATACCATCAAAGAAGCGGAGCGCGGCGTGGTGTTGCGTTTGGGGCAGTTCCATTCCATCGAACAACCGGGTTTAAACTGGAAACCGACCTTCATTg                                                                        <  1:443608/251‑1 (MQ=255)                                                                                                                                                                                     ttCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATACCATCAAAGAAGCGGAGCGCGGCGTGGTGTTGCGTTTGGGGCAGTTCCATTCCATCGAACAACCGGGTTTAAACTGGAAACCGACCTTCATTGATCGCGTCATTcc                                                           <  1:182714/251‑1 (MQ=255)                                                                                                                                                                                     ttCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTCTATACCATCAAAGAAGCGGAGCGCGGCGTGGTGTTGCGTTTGGGGCAGTTCCATTCCATCGAACAACCGGGTTTAAACTGGAAACCGACCTTCATTGATCGCGTCATTc                                                            >  1:877990/1‑250 (MQ=255)                                                                                                                                                                                              ttATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATACCATCAAAGAAGCGGAGCGCGGCGTGGTGTTGCGTTTGGGGCAGTTCCATTCCATCGAACAACCGGGTTTAAACTGGAAACCGACCTTCATTGATCGCGTCATTCCGGTCAAtgt                                                  <  1:124147/251‑1 (MQ=255)                                                                                                                                                                                                   gAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATACCATCAAAGAAGCGGAGCGCGGCGTGGTGTTGCGTTTGGGGCAGTTCCATTCCATCGAACAACCGGGTTTAAACTGGAAAc                                                                                    <  1:747456/212‑1 (MQ=255)                                                                                                                                                                                                    aaaaaaaTGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATACCATCAAAGAAGCGGAGCGCGGCGTGGTGTTGCGTTTGGGGCAGTTCCATTCCATCGAACAACCGGGTTTAAACTGGAAACCGACCTTCATTGATCGCGTCATTCCGGTCAATGTGGAAcg                                            <  1:498546/251‑1 (MQ=255)                                                                                                                                                                                                        aaaTGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATACCATCAAAGAAGCGGAGCGCGGCGTGGTGTTGCGTTTGGGGCAGTTCCATTCCATCGAACAACCGGGTTTAAACTGGAAACCGACCTTCATTGATCGCGTCATTCCGGTCAATGTGGAACGCGTa                                        >  1:779552/1‑251 (MQ=255)                                                                                                                                                                                                           tGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATACCATCAAAGAAGCGGAGCGCGGCGTGGTGTTGCGTTTGGGGCAGTTCCATTCCATCGAACAACCGGGTTTAAACTGGAAACCGACCTTCATTGATCGCGTCATTCCGGTCAATGTGGAACGCGTACAg                                     <  1:831976/251‑1 (MQ=255)                                                                                                                                                                                                            gggCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGGGATTTTATGGGGCGCCAGGGGTTTTTATACCATCAAAGAAGCGGAGCGCGGCGTGGTGTTGCGt                                                                                                                                  <  1:874563/157‑1 (MQ=255)                                                                                                                                                                                                                                 tAACAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATACCATCAAAGCAGCGGAGCGCGGCGTGGTGTTGCGTTTGTGGCAGTTCCATTCCATCGAACAACCGGGTTTAAACTGGAAACCGACCTTCATTGATCGCGTCATTCCGGTCAATGTGGAACGCGTACAGGAATTAAAAACTCAAGGCTCCa               >  1:655217/1‑251 (MQ=255)                                                                                                                                                                                                                                    cAATTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATACCATCAAAGAAGCGGAGCGCGGCGTGGTGTTGCGTTTGGGGCAGTTCCATTCCATCGAACAACCGGGTTTAAACTGGAAACCGACCTTCATTGATCGCGTCATTCCGGTCAATGTGGAACGCGTACAGGAATTAAAAACTCAAGGCTCCATg             >  1:264353/1‑250 (MQ=255)                                                                                                                                                                                                                                      aTTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATACCATCAAAGAAGCGGAGCGCGGCGTGGTGTTGCGTTTGGGGCAGTTCCATTCCATCGAACAACCGGGTTTAAAc                                                                                           >  1:189636/1‑170 (MQ=255)                                                                                                                                                                                                                                      aTTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATACCATCAAAGAAGCGGAGCGCGGCGTGGTGTTGCGTTTGGGGCAGTTCCATTCCATCGAACAACCGGGTTTAAACTGGAAACCGACCTTCAt                                                                          >  1:90886/1‑187 (MQ=255)                                                                                                                                                                                                                                      aTTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATACCATCAAAGAAGCGGAGCGCGGCGTGGTGTTGCGTTTGGGGCAGTTCCATTCCATCGAACAACCGGGTTTAAACTGGAAACCGACCTTCATTGATCGCGTCATTCCGGTCAATGTGGAACGCGTACAGGAATTAAAAACTCAAGGCTCCATg             >  1:19513/1‑248 (MQ=255)                                                                                                                                                                                                                                      aTTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATACCATCAAAGAAGCGGAGCGCGGCGTGGTGTTGCGTTTGGGGCAGTTCCATTCCATCGAACAACCGGGTTTAAACTGGAAACCGACCTTCATTGATCGCGTCATTCCGGTCAATGTGGAACGCGTACAGGAATTAAAAACTCAAGGCTCCATg             >  1:199186/1‑248 (MQ=255)                                                                                                                                                                                                                                      aTTCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATACCATCAAAGAAGCGGAGCGCGGCGTGGTGTTGCGTTTGGGGCAGTTCCATTCCATCGAACAACCGGGTTTAAACTGGAAACCGACCTTCATTGATCGCGTCATTCCGGTCAATGTGGAACGCGTACAGGAATTAAAAACTCAAGGCTCCATg             >  1:191565/1‑248 (MQ=255)                                                                                                                                                                                                                                       ttCCAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATACCATCAAAGAAGCGGAGCGCGGCGTGGTGTTGCGTTTGGGGCAGTTCCATTCCATCGAACAACCGGGTTTAAACTGGAAACCGACCTTCATTGATCGCGTCATTCCGGTCAATGTGGAACGCGTACAGGAATTAAAAACTCAAGGCTCCATGCTGa         >  1:586342/1‑251 (MQ=255)                                                                                                                                                                                                                                          cAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATACCATCAAAGAAGCGGAGCGCGGCGTGGTGTTGCGTTTGGGGCAGTTCCATTCCATCGAACAACCGGGTTTAAACTGGAAACCGACCTTCATTGATCGCGTCATTCCGGTCAATGTGGAACGCGTACAGGAATTAAAAACTCAAGGCTCCATGCTGacac      <  1:708589/251‑1 (MQ=255)                                                                                                                                                                                                                                          cAATCATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATACCATCAAAGAAGCGGAGCGCGGCGTGGTGTTGCGTTTGGGGCAGTTCCATTCCATCGAACAACCGGGTTTAAACTGGAAACCGACCTTCATTGATCGCGTCATTCCGGTCAATGTGGAACGCGTACAGGAATTAAAAACTCAAGGCTCCATGCTGacac      <  1:238338/251‑1 (MQ=255)                                                                                                                                                                                                                                              cATGCCAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATACCATCAAAGAAGCGGAGCGCGGCGTGGTGTTGCGTTTGGGGCAGTTCCATTCCATCGAACAACCGGGTTTAAACTGGAAACCGACCTTCATTGATCGCGTCATTCCGGTCAATGTGGAACGCGTACAGGAATTAAAAACTCAAGGCTCCATGCTGACACAAGa  >  1:699700/1‑251 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      CAAACGGAGAAAGCAATGTCGTTAAACGGTTCAGATCAAGATCCTTGGGGCAAACCAACGCAAAACGGACAATCGTCAGGAAACTCCTCTTCCGATAAAAACGAAGGTCAGTCCAATTGGGATCGTTCTTCCAATTCACAAAAAAAGAACGAGCAATCACCGCCCGATTTAGAAGAAGTTTTCAATAATTTATTGAAAAAAATGGGCGGGAAAGGCGCCAAAAATAACAATTCCAATCATGCTAATTTGCCTTCCGGCTTAGGTAAATTATTGCCGATTGCCATTGCGGCAGGCGTGATTTTATGGGGCGCCAGCGGTTTTTATACCATCAAAGAAGCGGAGCGCGGCGTGGTGTTGCGTTTGGGGCAGTTCCATTCCATCGAACAACCGGGTTTAAACTGGAAACCGACCTTCATTGATCGCGTCATTCCGGTCAATGTGGAACGCGTACAGGAATTAAAAACTCAAGGCTCCATGCTGACACAAGA  >  1/1257362‑1257849

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑