Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,252,893	A→G	P531P (CCA→CCG)	unknown →	Aspartokinase (EC 2.7.2.4) / Homoserine dehydrogenase (EC 1.1.1.3)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,252,893	0	A	G	100.0%	155.8 / NA	57	P531P (CCA→CCG)	unknown	Aspartokinase (EC 2.7.2.4) / Homoserine dehydrogenase (EC 1.1.1.3)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/25);  total (32/25)

CGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGTGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCATCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGTTTATACGAACGCGCCTTAGCGGAAGGGTTCCATGTGGTGACTCCGAATAAAAAAGCCAATACCCGCGCGTTGGATTATTATCACAAATTACGTGAACTGGCACATAAAAACCAACGTAAATTCCTGTATGAAAC  >  1/1252653‑1253120                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                    cGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCg                                                                                                                                                                                                                           >  1:239951/1‑251 (MQ=255)     aaaGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGt                                                                                                                                                                                                                       <  1:84845/251‑1 (MQ=255)        gCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGAc                                                                                                                                                                                                                         >  1:792392/1‑246 (MQ=255)        gCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTa                                                                                                                                                                                                                      >  1:491818/1‑249 (MQ=255)                 cAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCttt                                                                                                                                                                                                           <  1:137050/251‑1 (MQ=255)                 cAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCttt                                                                                                                                                                                                           <  1:274870/251‑1 (MQ=255)                    gCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCAt                                                                                                                                                                                                        >  1:709893/1‑251 (MQ=255)                    gCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGCCGGATTTCGACGTACTGCTCTCTTTCAt                                                                                                                                                                                                        >  1:210181/1‑251 (MQ=255)                       ttATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTaa                                                                                                                                                                                                     >  1:545017/1‑251 (MQ=255)                               taaCAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGcatc                                                                                                                                                                                             <  1:632683/251‑1 (MQ=255)                                  cAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCAtg                                                                                                                                                                                          >  1:361825/1‑251 (MQ=255)                                         aTCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGa                                                                                                                                                                                    <  1:692739/250‑1 (MQ=255)                                                  gtgtTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGGCTTAGAAAACGCCACCCAACCGTCGGATTTCGAc                                                                                                                                                                                                                         >  1:437277/1‑204 (MQ=255)                                                  gtgtTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCttt                                                                                                                                                                                                           >  1:172154/1‑218 (MQ=255)                                                      ttCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTg                                                                                                                                                                      <  1:8451/251‑1 (MQ=255)                                                        cTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCttt                                                                                                                                                                                                           >  1:326746/1‑212 (MQ=255)                                                        cTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCAtg                                                                                                                                                                                          >  1:394612/1‑229 (MQ=255)                                                              ggtgtcggcggtgtcggcGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTg                                                                                                                                                                 >  1:66677/1‑248 (MQ=255)                                                                tgtcggcggtgtcggcggCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCttt                                                                                                                                                                                                           >  1:168506/1‑204 (MQ=255)                                                                                 gCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGc                                                                                                                                            <  1:407087/250‑1 (MQ=255)                                                                                  cGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGcc                                                                                                                                           <  1:236874/250‑1 (MQ=255)                                                                                   gAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGcat                                                                                                                                                                                              >  1:665160/1‑198 (MQ=255)                                                                                    aattaattGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGt                                                                                                                                        >  1:659385/1‑251 (MQ=255)                                                                                    aattaattGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGt                                                                                                                                        >  1:787732/1‑251 (MQ=255)                                                                                       taattGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCAtgt                                                                                                                                                                                         <  1:468670/199‑1 (MQ=255)                                                                                           tGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCGTTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGTTTATACg                                                                                                                                 <  1:671305/251‑1 (MQ=255)                                                                                           tGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGTTTATACg                                                                                                                                 <  1:288547/251‑1 (MQ=255)                                                                                                  aTCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGTTTATACGAAcgcg                                                                                                                           >  1:391159/1‑250 (MQ=255)                                                                                                          ccAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGTTTATACGAACGCGCCTTAGCgg                                                                                                                  >  1:340603/1‑251 (MQ=255)                                                                                                               aaGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGTTTATACGAACGCGCCTTAGCGGAAggg                                                                                                             <  1:175492/251‑1 (MQ=255)                                                                                                                      tttAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTAGGCCGGTTTATACGAACGCGCCTTAGCGGAAGGGTTCCAtg                                                                                                      <  1:675233/251‑1 (MQ=255)                                                                                                                             aagaaagaCATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTc                                                                                                                                                           >  1:494223/1‑191 (MQ=255)                                                                                                                             aagaaagaCATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGTTTATACGAACGCGCCTTAGCGGAAGGGTTCCATGTGGTGa                                                                                                >  1:89696/1‑250 (MQ=255)                                                                                                                               gaaagaCATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGTTTATACGAACGCGCCTTAGCGGAAGGGTTCCATGTGGTGACt                                                                                              >  1:152935/1‑250 (MQ=255)                                                                                                                                   gaCATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTa                                                                                                                                                                                                      >  1:305033/1‑142 (MQ=255)                                                                                                                                    aCATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGTTTATACGAACGCGCCTTAGCGGAAGGGTTCCAt                                                                                                       >  1:172151/1‑236 (MQ=255)                                                                                                                                          aaaTTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGTTTATACGAACGCGCCTTAGCGGAAGGGTTCCATGTGGTGACTCCGAATaaaaa                                                                                   <  1:577003/250‑1 (MQ=255)                                                                                                                                               cgcgTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGTTTATACGAACGCGCCTTAGCGGAAGGGTTCCATGTGGTGACTCCGAATAAAAAAGCCaa                                                                             >  1:650428/1‑251 (MQ=255)                                                                                                                                                   tttGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGTTTATACGAACGCGCCTTAGCGGAAGGGTTCCATGTGGTGACTCCGAATAAAAAAGCCAATAcc                                                                         <  1:501040/251‑1 (MQ=255)                                                                                                                                                           cTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTAc                                                                                                                                                                                                                     <  1:244988/103‑1 (MQ=255)                                                                                                                                                           cTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGTTTATACGAACGCGCCTTAGCGGAAGGGTTCCATgtgg                                                                                                   >  1:209089/1‑217 (MQ=255)                                                                                                                                                                   ttCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGcc                                                                                                                                           <  1:335512/169‑1 (MQ=255)                                                                                                                                                                                ttGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGTTTATACGAACGCGCCTTAGCGGAAGGGTTCCATGTGGTGACTCCGAATAAAAAAGCCAATACCCGCGCGTTGGATTATTATCACAAATTACg                                            <  1:353488/251‑1 (MQ=255)                                                                                                                                                                                   cTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGTTTATACGAACGCGCCTTAGCGGAAGGGTTCCATGTGGTGACTCCGAATAAAAAAGCCAATACCCGCGCGTTGGATTATTATCACAAATTACGTg                                          <  1:289605/250‑1 (MQ=255)                                                                                                                                                                                       aTGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGTTTATACGAACGCGCCTTGGCGGAAGGGTTCCATGTGGTGACTCCGAATAAAAAAGCCAATACCCGCGCGTTGGATTATTATCACAAATTACGTGaa                                        >  1:447280/1‑248 (MQ=255)                                                                                                                                                                                       aTGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGTTTATACGAACGCGCCTTAGCGGAAGGGTTCCATGTGGTGACTCCGAATAAAAAAGCCAATACCCGCGCGTTGGATTATTATCACAAATTACGTGaa                                        >  1:853785/1‑248 (MQ=255)                                                                                                                                                                                         gAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGTTTATACGAACGCGCCTTAGCGGAAGGGTTCCATgtgg                                                                                                   >  1:486566/1‑187 (MQ=255)                                                                                                                                                                                             aCGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGTTTATACGAACGCGCCTTAGCGGAAGGGTTCCATGTGGTGACTCCGAATAAAAAAGCCAATACCCGCGCGTTGGATTATTATCACAAATTACGTGAACTGGCACAt                               <  1:616181/251‑1 (MQ=255)                                                                                                                                                                                                   tAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGTTTATACGAACGCGCCTTAGCGGAAGGGTTCCATGTGGTGACTCCGAATAAAAAAGCCAATACCCGCGCGTTGGATTATTATCACAAATTACGTGAACTGGCACATAAAAAc                         <  1:216027/251‑1 (MQ=255)                                                                                                                                                                                                    aaaTTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGTTTATACGAACGCGCCTTAGCGGAAGGGTTCCATGTGGTGACTCCGAATAAAAAAGCCAATACCCGCGCGTTGGATTATTATCACAAATTACGTGAACTGGCACATAAAAAcc                        <  1:112014/251‑1 (MQ=255)                                                                                                                                                                                                     aaTTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGTTTATACg                                                                                                                                 <  1:774093/145‑1 (MQ=255)                                                                                                                                                                                                     aaTTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGTTTATACGAACGCGCCTTAGCGGAAGGGTTCCATgtgg                                                                                                   >  1:663592/1‑175 (MQ=255)                                                                                                                                                                                                      aTTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGTTTATACGAACGCGCCTTAGCGGAAGGGTTCCATgtggtg                                                                                                 >  1:16375/1‑176 (MQ=255)                                                                                                                                                                                                                ttGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGTTTATACGAACGCGCCTTAGCGGAAGGGTTCCATGTGGTGACTCCGAATAAAAAAGCCAATACCCGCGCGTTGGATTATTATCACAAATTACGTGAACTGGCACATAAAAACCAACGTAAATTcc            <  1:426923/251‑1 (MQ=255)                                                                                                                                                                                                                    cAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGTTTATACGAACGCGCCTTAGCGGAAGGGTTCCATGTGGTGACTCCGAATAAAAAAGCCAATACCCGCGCGTTGGATTATTATCACAAATTACGTGAACTGGCACATAAAAACCAACGTAAATTCCTGTa        >  1:144441/1‑251 (MQ=255)                                                                                                                                                                                                                          gACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGTTTATACGAACGCGCCTTAGCGGAAGGGTTCCATGTGGTGACTCCGAATAAAAAAGCCAATACCCGCGCGTTGGATTATTATCACAAATTACGTGAACTGGCACATAAAAACCAACGTAAATTCCTGTATGaaa   >  1:170812/1‑250 (MQ=255)                                                                                                                                                                                                                           aCTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGTTTATACGAACGCGCCTTAGCGGAAGGGTTCCATGTGGTGACTCCGAATAAAAAAGCCAATACCCGCGCGTTGGATTATTATCACAAATTACGTGAACTGGCACATAAAAACCAACGTAAATTCCTGTATGAAAc  <  1:23670/250‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                    CGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGTGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCATCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTGCACGTCGGCGGAATCCTTGGCCGGTTTATACGAACGCGCCTTAGCGGAAGGGTTCCATGTGGTGACTCCGAATAAAAAAGCCAATACCCGCGCGTTGGATTATTATCACAAATTACGTGAACTGGCACATAAAAACCAACGTAAATTCCTGTATGAAAC  >  1/1252653‑1253120

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑