Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,252,731	T→C	G477G (GGT→GGC)	unknown →	Aspartokinase (EC 2.7.2.4) / Homoserine dehydrogenase (EC 1.1.1.3)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,252,731	0	T	C	100.0%	142.0 / NA	53	G477G (GGT→GGC)	unknown	Aspartokinase (EC 2.7.2.4) / Homoserine dehydrogenase (EC 1.1.1.3)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/19);  total (34/19)

GGTATGCGCCAAGCTAAAGGCATTGCCGCGCGCTTCTTCTCTGCGCTGGCACAGGCAAACATCAGCATTAACGCCATTGCCCAAGGTTCTTCCGAACGTTCAATTTCCGCCGTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGTGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCATCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTG  >  1/1252513‑1252961                                                                                                                                                                                                                           |                                                                                                                                                                                                                                       ggTATGCGCCAAGCTAAAGGCATTGCCGCGCGCTTCTTCTCTGCGCTGGCACAGGCAAACATCAGCATTAACGCCATTGCCCAAGGTTCTTCCGAACGTTCAATTTCCGCCGTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCaaaa                                                                                                                                                                                                        >  1:825391/1‑251 (MQ=255)   tATGCGCCAAGCTAAAGGCATTGCCGCGCGCTTCTTCTCTGCGCTGGCACAGGCAAACATCAGCATTAACGCCATTGCCCAAGGTTCTTCCGAACGTTCAATTTCCGCCGTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAag                                                                                                                                                                                                      <  1:356413/251‑1 (MQ=255)            aGCTAAAGGCATTGCCGCGCGCTTCTTCTCTGCGCTGGCACAGGCAAACATCAGCATTAACGCCATTGCCCAAGGTTCTTCCGAACGTTCAATTTCCGCCGTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGGTCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGc                                                                                                                                                                                                                        >  1:444769/1‑224 (MQ=255)             gCTAAAGGCATTGCCGCGCGCTTCTTCTCTGCGCTGGCACAGGCAAACATCAGCATTAACGCCATTGCCCAAGGTTCTTCCGAACGTTCAATTTCCGCCGTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTAttt                                                                                                                                                                                               >  1:246558/1‑248 (MQ=255)                   ggCATTGCCGCGCGCTTCTTCTCTGCGCTGGCACAGGCAAACATCAGCATTAACGCCATTGCCCAAGGTTCTTCCGAACGTTCAATTTCCGCCGTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCaaagaa                                                                                                                                                                                      >  1:272269/1‑251 (MQ=255)                               cgcTTCTTCTCTGCGCTGGCACAGGCAAACATCAGCATTAACGCCATTGCCCAAGGTTCTTCCGAACGTTCAATTTCCGCCGTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGAGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGaaa                                                                                                                                                                           <  1:771168/250‑1 (MQ=255)                                 cttcttCTCTGCGCTGGCACAGGCAAACATCAGCATTAACGCCATTGCCCAAGGTTCTTCCGAACGTTCAATTTCCGCCGTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGGATTTCGCAAAGAAAGACATTGAAAtt                                                                                                                                                                         >  1:736036/1‑250 (MQ=255)                                      tctcTGCGCTGGCACAGGCAAACATCAGCATTAACGCCATTGCCCAAGGTTCTTCCGAACGTTCAATTTCCGCCGTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGtt                                                                                                                                                                   >  1:144354/1‑251 (MQ=255)                                           gcgcTGGCACAGGCAAACATCAGCATTAACGCCATTGCCCAAGGTTCTTCCGAACGTTCAATTTCCGCCGTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTgcg                                                                                                                                                               >  1:181878/1‑250 (MQ=255)                                                ggCACAGGCAAACATCAGCATTAACGCCATTGCCCAAGGTTCTTCCGAACGTTCAATTTCCGCCGTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCAGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTcg                                                                                                                                                         >  1:442698/1‑251 (MQ=255)                                                      ggCAAACATCAGCATTAACGCCATTGCCCAAGGTTCTTCCGAACGTTCAATTTCCGCCGTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAacac                                                                                                                                                                                                             >  1:743375/1‑193 (MQ=255)                                                            cATCAGCATTAACGCCATTGCCCAAGGTTCTTCCGAACGTTCAATTTCCGCCGTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCcaaca                                                                                                                                             >  1:299695/1‑251 (MQ=255)                                                             aTCAGCATTAACGCCATTGCCCAAGGTTCTTCCGAACGTTCAATTTCCGCCGTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCcaacaa                                                                                                                                            <  1:861360/251‑1 (MQ=255)                                                                 gCATTAACGCCATTGCCCAAGGTTCTTCCGAACGTTCAATTTCCGCCGTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATg                                                                                                                                        >  1:752013/1‑251 (MQ=255)                                                                  cATTAACGCCATTGCCCAAGGTTCTTCCGAACGTTCAATTTCCGCCGTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGt                                                                                                                                       <  1:877725/251‑1 (MQ=255)                                                                       aCGCCATTGCCCAAGGTTCTTCCGAACGTTCAATTTCCGCCGTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTc                                                                                                                                  <  1:819680/251‑1 (MQ=255)                                                                               gCCCAAGGTTCTTCCGAACGTTCAATTTCCGCCGTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCGAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGaaaa                                                                                                                          >  1:432372/1‑251 (MQ=255)                                                                               gCCCAAGGTTCTTCCGAACGTTCAATTTCCGCCGTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTc                                                                                                                                  >  1:662848/1‑243 (MQ=255)                                                                               gCCCAAGGTTCTTCCGAACGTTCAATTTCCGCCGTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGaaaa                                                                                                                          >  1:161909/1‑251 (MQ=255)                                                                                          ttcCGAACGTTCAATTTCCGCCGTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAAtt                                                                                                               <  1:512841/251‑1 (MQ=255)                                                                                           tcCGAACGTTCAATTTCCGCCGTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAAttt                                                                                                              <  1:539376/251‑1 (MQ=255)                                                                                               aaCGTTCAATTTCCGCCGTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGAt                                                                                                          <  1:237355/251‑1 (MQ=255)                                                                                                aCGTTCAATTTCCGCCGTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATc                                                                                                         <  1:283559/251‑1 (MQ=255)                                                                                                aCGTTCAATTTCCGCCGTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATc                                                                                                         <  1:289320/251‑1 (MQ=255)                                                                                                            cgccgTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAAt                                                                                                                                                    >  1:497614/1‑196 (MQ=255)                                                                                                                           aaaTAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAAtt                                                                                                               >  1:581706/1‑218 (MQ=255)                                                                                                                           aaaTAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCa                                                                              >  1:703874/1‑251 (MQ=255)                                                                                                                                  gCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAAc                                                                        >  1:51329/1‑250 (MQ=255)                                                                                                                                    cATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTTTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCCTGCGAATTCCAACAAAATGTTGGTCAATGAAAACGGCTTAAATTTTGATCATTGGTAAACGGACTTAGAAAACGCCACCCaaacg                                                                      >  1:231129/1‑247 (MQ=255)                                                                                                                                             cGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCcaac                                                                                                                                              <  1:511247/169‑1 (MQ=255)                                                                                                                                             cGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCg                                                            >  1:239951/1‑251 (MQ=255)                                                                                                                                                 aaaGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGt                                                        <  1:84845/251‑1 (MQ=255)                                                                                                                                                    gCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGAc                                                          >  1:792392/1‑246 (MQ=255)                                                                                                                                                    gCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTa                                                       >  1:491818/1‑249 (MQ=255)                                                                                                                                                          cACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAAccg                                                                      >  1:891470/1‑227 (MQ=255)                                                                                                                                                             cAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCttt                                            <  1:137050/251‑1 (MQ=255)                                                                                                                                                             cAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCttt                                            <  1:274870/251‑1 (MQ=255)                                                                                                                                                                gCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCAt                                         >  1:709893/1‑251 (MQ=255)                                                                                                                                                                gCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGCCGGATTTCGACGTACTGCTCTCTTTCAt                                         >  1:210181/1‑251 (MQ=255)                                                                                                                                                                   ttATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTaa                                      >  1:545017/1‑251 (MQ=255)                                                                                                                                                                     aTTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGAt                                                                                                          >  1:302739/1‑181 (MQ=255)                                                                                                                                                                     aTTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGaa                                                                                     <  1:88039/202‑1 (MQ=255)                                                                                                                                                                     aTTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAAc                                                                        >  1:800440/1‑215 (MQ=255)                                                                                                                                                                           taaCAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGcatc                              <  1:632683/251‑1 (MQ=255)                                                                                                                                                                              cAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGaa                                                                                     <  1:336345/193‑1 (MQ=255)                                                                                                                                                                              cAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCAtg                           >  1:361825/1‑251 (MQ=255)                                                                                                                                                                                     aTCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGa                     <  1:692739/250‑1 (MQ=255)                                                                                                                                                                                        gTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTg                                                                                                                                     >  1:424957/1‑135 (MQ=255)                                                                                                                                                                                              gtgtTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGGCTTAGAAAACGCCACCCAACCGTCGGATTTCGAc                                                          >  1:437277/1‑204 (MQ=255)                                                                                                                                                                                              gtgtTTCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCttt                                            >  1:172154/1‑218 (MQ=255)                                                                                                                                                                                                  ttCTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTg       <  1:8451/251‑1 (MQ=255)                                                                                                                                                                                                    cTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCttt                                            >  1:326746/1‑212 (MQ=255)                                                                                                                                                                                                    cTTGTGGGTGTCGGCGGTGTCGGCGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCAtg                           >  1:394612/1‑229 (MQ=255)                                                                                                                                                                                                          ggtgtcggcggtgtcggcGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTg  >  1:66677/1‑248 (MQ=255)                                                                                                                                                                                                            tgtcggcggtgtcggcggCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCGTCGGATTTCGACGTACTGCTCTCttt                                            >  1:168506/1‑204 (MQ=255)                                                                                                                                                                                                                           |                                                                                                                                                                                                                                       GGTATGCGCCAAGCTAAAGGCATTGCCGCGCGCTTCTTCTCTGCGCTGGCACAGGCAAACATCAGCATTAACGCCATTGCCCAAGGTTCTTCCGAACGTTCAATTTCCGCCGTGGTGGCGCAAAATAAAGCCATTGAGGCCGTAAAAGCAACGCACCAAGCGTTATTTAATAACAAAAAGATCGTTGAAGTGTTTCTTGTGGGTGTCGGCGGTGTCGGTGGCGAATTAATTGAGCAAATCAAACACCAAAAAGAGTATTTAGCAAAGAAAGACATTGAAATTCGCGTTTGCGCCCTCGCGAATTCCAACAAAATGTTGCTCAATGAAAACGGCTTAAATTTAGATCATTGGCAAACGGACTTAGAAAACGCCACCCAACCATCGGATTTCGACGTACTGCTCTCTTTCATTAAATTGCATCATGTGGTGAACCCGGTTTTCGTGGATTG  >  1/1252513‑1252961

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑