Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,247,439	A→G	H430R (CAC→CGC)	unknown →	Glycine betaine ABC transport system, permease protein OpuAB / Glycine betaine ABC transport system, glycine betaine‑binding protein OpuAC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,247,439	0	A	G	100.0%	207.1 / NA	76	H430R (CAC→CGC)	unknown	Glycine betaine ABC transport system, permease protein OpuAB / Glycine betaine ABC transport system, glycine betaine‑binding protein OpuAC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (46/30);  total (46/30)

ACACATGGGCTTGCAACAATCAGCGATTTACATCATGTGGCAGGTGACATTCGCGCCGGCTTCACACTGGAATTTCTCGATCGAGTCGATGGCTATAAAGGAATAAAATCACAAGGCATTACTTTAGTTCATATTGTCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCACTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGAACAAGAAATGAGTGAAATGAATTATCAAGTTAAAGTACTGGGCGAAAGCCCGGAGAAAGTGGCGAGGGAGTATTTGAAGAAAATGAAATTGCTATAGCCTTAGACAAACGGTACCGGGC  >  1/1247201‑1247684                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                      acacATGGGCTTGCAACAATCAGCGATTTACATCATGTGGCAGGTGACATTCGCGCCGGCTTCACACTGGAATTTCTCGATCGAGTCGATGGCTATAAAGGAATAAAATCACAAGGCATTAGTTTAGTTCATATTGTCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAga                                                                                                                                                                                                                                           >  1:583701/1‑251 (MQ=255) acacATGGGCTTGCAACAATCAGCGATTTACATCATGTGGCAGGTGACATTCGCGCCGGCTTCACACTGGAATTTCTCGATCGAGTCGATGGCTATAAAGGAATAAAATCACAAGGCATTAGTTTAGTTCATATTGTCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAga                                                                                                                                                                                                                                           >  1:725171/1‑251 (MQ=255)  cacaTGGGCTTGCAACAATCAGCGATTTACATCATGTGGCAGGTGACATTCGCGCCGGCTTCACACTGGAATTTCTCGATCGAGTCGATGGCTATAAAGGAATAAAATCACAAGGCATTAGTTTAGTTCATATTGTCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAgac                                                                                                                                                                                                                                          <  1:827319/251‑1 (MQ=255)              caacaaTCAGCGATTTACATCATGTGGCAGGTGACATTCGCGCCGGCTTCACACTGGAATTTCTCGATCGAGTCGATGGCTATAAAGGAATAAAATCACAAGGCATTAGTTTAGTTCATATTGTCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAAttt                                                                                                                                                                                                                               <  1:24468/250‑1 (MQ=255)                acaaTCAGCGATTTACATCATGTGGCAGGTGACATTCGCGCCGGCTTCACACTGGAATTTCTCGATCGAGTCGATGGCTATAAAGGAATAAAATCACAAGGCATTAGTTTAGTTCATATTGTCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTAtt                                                                                                                                                                                                                            >  1:85715/1‑251 (MQ=255)                 caaTCAGCGATTTACATCATGTGGCAGGTGACATTCGCGCCGGCTTCACACTGGAATTTCTCGATCGAGTCGATGGCTATAAAGGAATAAAATCACAAGGCATTAGTTTAGTTCATATTGTCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTc                                                                                                                                                                                                                           <  1:738203/251‑1 (MQ=255)                          aTTTACATCATGTGGCAGGTGACATTCGCGCCGGCTTCACACTGGAATTTCTCGATCGAGTCGATGGCTATAAAGGAATAAAATCACAAGGCATTAGTTTAGTTCATATTGTCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACAtt                                                                                                                                                                                                                                    >  1:231225/1‑233 (MQ=255)                          aTTTACATCATGTGGCAGGTGACATTCGCGCCGGCTTCACACTGGAATTTCTCGATCGAGTCGATGGCTATAAAGGAATAAAATCACAAGGCATTAGTTTAGTTCATATTGTCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTAt                                                                                                                                                                                                                  >  1:547176/1‑251 (MQ=255)                             tACATCATGTGGCAGGTGACATTCGCGCCGGCTTCACACTGGAATTTCTCGATCGAGTCGATGGCTATAAAGGAATAAAATCACAAGGCATTAGTTTAGTTCATATTGTCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCaa                                                                                                                                                                                                               <  1:525730/251‑1 (MQ=255)                               catcatGTGGCAGGTGACATTCGCGCCGGCTTCACACTGGAATTTCTCGATCGAGTCGATGGCTATAAAGGAATAAAATCACAAGGCATTAGTTTAGTTCATATTGTCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAgg                                                                                                                                                                                                             <  1:70151/251‑1 (MQ=255)                               catcatGTGGCAGGTGACATTCGCGCCGGCTTCACACTGGAATTTCTCGATCGAGTCGATGGCTATAAAGGAATAAAATCACAAGGCATTAGTTTAGTTCATATTGTCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAgg                                                                                                                                                                                                             <  1:818342/251‑1 (MQ=255)                                        gCAGGTGACATTCGCGCCGGCTTCACACTGGAATTTCTCGATCGAGTCGATGGCTATAAAGGAATAAAATCACAAGGCATCAGTTTAGTTCATATTGTCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCAc                                                                                                                                                                                                     <  1:440554/250‑1 (MQ=255)                                          aGGTGACATTCGCGCCGGCTTCACACTGGAATTTCTCGATCGAGTCGATGGCTATAAAGGAATAAAATCACAAGGCATTAGTTTAGTTCATATTGTCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTaa                                                                                                                                                                                                  >  1:736702/1‑251 (MQ=255)                                           ggTGACATTCGCGCCGGCTTCACACTGGAATTTCTCGATCGAGTCGATGGCTATAAAGGAATAAAATCACAAGGCATTAGTTTAGTTCATATTGTCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAAt                                                                                                                                                                                                 >  1:163211/1‑251 (MQ=255)                                             tGACATTCGCGCCGGCTTCACACTGGAATTTCTCGATCGAGTCGATGGCTATAAAGGAATAAAATCACAAGGCATTAGTTTAGTTCATATTGTCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATg                                                                                                                                                                                                <  1:472017/250‑1 (MQ=255)                                                           gCTTCACACTGGAATTTCTCGATCGAGTCGATGGCTATAAAGGAATAAAATCACAAGGCATTAGTTTAGTTCATATTGTCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTgca                                                                                                                                                                                 >  1:561356/1‑251 (MQ=255)                                                                 cacTGGAATTTCTCGATCGAGTCGATGGCTATAAAGGAATAAAATCACAAGGCATTAGTTTAGTTCATATTGTCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCaaaa                                                                                                                                                                           >  1:418443/1‑251 (MQ=255)                                                                             tcgatcgaGTCGATGGCTATAAAGGAATAAAATCACAAGGCATTAGTTTAGTTCATATTGTCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGtt                                                                                                                                                               <  1:696226/251‑1 (MQ=255)                                                                                            gCTATAAAGGAATAAAATCACAAGGCATTAGTTTAGTTCATATTGTCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTaa                                                                                                                                                 >  1:207179/1‑250 (MQ=255)                                                                                            gCTATAAAGGAATAAAATCACAAGGCATTAGTTTAGTTCATATTGTCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTaa                                                                                                                                                 >  1:857517/1‑250 (MQ=255)                                                                                             cTATAAAGGAATAAAATCACAAGGCATTAGTTTAGTTCATATTGTCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAAc                                                                                                                                                >  1:423928/1‑250 (MQ=255)                                                                                                     gAATAAAATCACAAGGCATTAGTTTAGTTCATATTGTCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGc                                                                                                                                        >  1:472300/1‑250 (MQ=255)                                                                                                                   ggCATTAGTTTAGTTCATATTGTCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTATGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAAc                                                                                                                                                >  1:868065/1‑228 (MQ=255)                                                                                                                    gCATTAGTTTAGTTCATATTGTCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAgg                                                                                                                                                                                                             >  1:411598/1‑166 (MQ=255)                                                                                                                    gCATTAGTTTAGTTCATATTGTCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTc                                                                                                                            >  1:245905/1‑247 (MQ=255)                                                                                                                    gCATTAGTTTAGTTCATATTGTCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGaa                                                                                                                        >  1:316814/1‑251 (MQ=255)                                                                                                                    gCATTAGTTTAGTTCATATTGTCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGaa                                                                                                                        >  1:727400/1‑251 (MQ=255)                                                                                                                         agtttAGTTCATATTGTCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGAACAAGa                                                                                                                   >  1:348508/3‑251 (MQ=255)                                                                                                                          gtttAGTTCATATTGTCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCg                                                                                                                                                                                         >  1:90635/2‑180 (MQ=255)                                                                                                                          gtttAGTTCATATTGTCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGAACAAGaa                                                                                                                  >  1:567240/2‑251 (MQ=255)                                                                                                                          gtttAGTTCATATTGTCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGAACAAGaa                                                                                                                  >  1:716610/2‑251 (MQ=255)                                                                                                                          gtttAGTTCATATTGTCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGAACAAGaa                                                                                                                  >  1:527107/2‑251 (MQ=255)                                                                                                                              aGTTCATATTGTCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTc                                                                                                                                                                                                                           <  1:466729/142‑1 (MQ=255)                                                                                                                                ttCATATTGTCCCCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGAACAAGAAATGAGt                                                                                                            <  1:828239/251‑1 (MQ=255)                                                                                                                                ttCATATTGTCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGAACAAGAAATGAGt                                                                                                            <  1:137465/251‑1 (MQ=255)                                                                                                                                ttCATATTGTCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACACAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACAACAGCCCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCAATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGAACAAGAAATGAGt                                                                                                            <  1:334599/251‑1 (MQ=255)                                                                                                                                    tatTGTCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTaa                                                                                                                                                                                                  >  1:830884/1‑161 (MQ=255)                                                                                                                                    tatTGTCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGAACAAGAAATGAGTGaaa                                                                                                        >  1:881344/1‑251 (MQ=255)                                                                                                                                    tatTGTCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTAAACCGCCTCGCCAATCGTATTTCCGAACAAGAAATGAGTGaaa                                                                                                        >  1:227991/1‑251 (MQ=255)                                                                                                                                      ttGTCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGAACAAGAAATGAGTGAAATg                                                                                                      >  1:461628/1‑251 (MQ=255)                                                                                                                                        gTCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGAACAAGAAATGAGTGAAATGaa                                                                                                    >  1:63403/1‑251 (MQ=255)                                                                                                                                         tCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGAACAAGAAATGAGTGAAATGAAt                                                                                                   <  1:165161/251‑1 (MQ=255)                                                                                                                                         tCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGAACAAGAAATGAGTGAAATGAAt                                                                                                   <  1:812731/251‑1 (MQ=255)                                                                                                                                           aCCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGAACAAGAAATGAGTGAAATGAATTa                                                                                                 <  1:680132/251‑1 (MQ=255)                                                                                                                                            cccTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCAc                                                                                                                                                                                                     >  1:543717/1‑150 (MQ=255)                                                                                                                                            cccTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATc                                                                                                                                                                       >  1:686520/1‑180 (MQ=255)                                                                                                                                            cccTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGAACAAGAAATGAGTGAAATGAATTAt                                                                                                >  1:621380/1‑251 (MQ=255)                                                                                                                                                       gCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCg                                                                                                                                              >  1:223035/1‑194 (MQ=255)                                                                                                                                                        cTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGAACAAGAAATGAGTGAAATGAATTATCAAGTTAAAGt                                                                                     <  1:335133/250‑1 (MQ=255)                                                                                                                                                         tttGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGAACAAGAAATGAGTGAAATGAATTATCAAGTTAAAGTAc                                                                                   <  1:774135/251‑1 (MQ=255)                                                                                                                                                         tttGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGAACAAGAAATGAGTGAAATGAATTATCAAGTTAAAGTAc                                                                                   <  1:414584/251‑1 (MQ=255)                                                                                                                                                         tttGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGAACAAGAAATGAGTGAAATGAATTATCAAGTTAAAGTAc                                                                                   <  1:31253/251‑1 (MQ=255)                                                                                                                                                            gCGTTATACGGCGTTACGCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAAc                                                                                                                                                <  1:431950/187‑1 (MQ=255)                                                                                                                                                              gTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAACAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGAACAAGAAATGAGTGAAATGAATTATCAAGTTAAAGTACTggg                                                                               >  1:514969/1‑250 (MQ=255)                                                                                                                                                                 ataCGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATCAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGAACAAGAAATGAGTGAAATGAATTATCAAGTTAAAGt                                                                                     >  1:589005/1‑241 (MQ=255)                                                                                                                                                                         ttACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGAACAAGAAATGAGTGAAATGAATTATCAAGTTAAAGTACTGGGCGAAAGCCCGga                                                                   >  1:619288/1‑251 (MQ=255)                                                                                                                                                                              caacaaTAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCCGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGAACAAGAAATGAGTGAAATGAATTATCAAGTTAAAGTACTGGGCGAAAGCCCGGAGAAAg                                                              >  1:660529/1‑251 (MQ=255)                                                                                                                                                                                acaaTAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGAACAAGAAATGAGTGAAATg                                                                                                      >  1:263841/1‑209 (MQ=255)                                                                                                                                                                                 caaTAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCaa                                                                                                                                                                                                               >  1:140859/1‑103 (MQ=255)                                                                                                                                                                                   aTAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGAACAAGAAATGAGTGAAATGAATTATCAAGTTAAAGTACTGGGCGAAAGCCCGGAGAAAGTGGCg                                                         >  1:381260/1‑251 (MQ=255)                                                                                                                                                                                      aaaTTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGAACAAGAAATGAGTGAAATGAATTATCAAGTTAAAGTAc                                                                                   <  1:764329/222‑1 (MQ=255)                                                                                                                                                                                      aaaTTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGAACAAGAAATGAGTGAAATGAATTATCAAGTTAAAGTACTGGGCGAAAGCCCGGAGAAAGTGGCGAgg                                                      >  1:790744/1‑251 (MQ=255)                                                                                                                                                                                                         aTTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGAACAAGAAATGAGTGAAATGAATTATCAAGTTAAAGTAc                                                                                   >  1:290832/1‑203 (MQ=255)                                                                                                                                                                                                         aTTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGAACAAGAAATGAGTGAAATGAATTATCAAGTTAAAGTACTGGGCGAAAGCCCGGAGAAAGTGGCGAGGGAGTATTTgaaga                                         <  1:300025/245‑1 (MQ=255)                                                                                                                                                                                                                      aacagaCTCAAACACCATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAGGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGAACAAGAAATGAGTGAAATGAATTATCAAGTTAAAGTACTGGGCGAAAGCCCGGAGAAAGTGGCGAGGGAGTATTTGAAGAAAATGAAATTGCTATAGcc                      <  1:355177/246‑1 (MQ=255)                                                                                                                                                                                                                       cAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGAACAAGAAATGAGTGAAATGAATTATCAAGTTAAAGTACTGGGCGAAAGCCCGGAGAAAGTGGCGAGGGAGTATTTGAAGAAAATGAAATTGCTATAGcc                      <  1:329935/250‑1 (MQ=255)                                                                                                                                                                                                                       cAGAACTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGAACAAGAAATGAGTGAAATGAATTATCAAGTTAAAGTACTGGGCGAAAGCCCGGAGAAAGTGGCGAGGGAGTATTTGAAGAAAATGAAATTGCTATAGcc                      <  1:463272/250‑1 (MQ=255)                                                                                                                                                                                                                           aCTCAAACAACATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGAACAAGAAATGAGTGAAATGAATTATCAAGTTAAAGTACTGGGCGAAAGCCCGGAGAAAGTGGCGAGGGAGTATTTGAAGAAAATGAAATTGCTATAGCCTTAGa                 >  1:5123/1‑251 (MQ=255)                                                                                                                                                                                                                                aacaacATCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGAACAAGAAATGAGTGAAATGAATTATCAAGTTAAAGTACTGGGCGAAAGCCCGGAGAAAGTGGCGAGGGAGTATTTGAAGAAAATGAAATTGCTATAGCCTTAGACaaa             >  1:551164/1‑250 (MQ=255)                                                                                                                                                                                                                                  caacaTCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGAACAAGAAATGAGTGAAATGAATTATCAAGTTAAAGTACTGGGCGAAAGCCCGGAGAAAGTGGCGAGGGAGTATTTGAAGAAAATGAAATTGCTATAGCCTTAGACAAACgg          >  1:403404/1‑251 (MQ=255)                                                                                                                                                                                                                                    acaTCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGAACAAGAAATGAGTGAAATGAATTATCAAGTTAAAGTACTGGGCGAAAGCCCGGAGAAAGTGGCGAGGGAGTAtt                                               <  1:496821/212‑1 (MQ=255)                                                                                                                                                                                                                                     caTCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCaa                                                                                                                                                                                                               <  1:244583/51‑1 (MQ=255)                                                                                                                                                                                                                                     caTCAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGAACAAGAAATGAGTGAAATGAATTATCAAGTTAAAGTACTGGGCGAAAGCCCGGAGAAAGTGGCGAGGGAGTATTTGAAGAAAATGAAATTGCTATAGcc                      >  1:73766/1‑236 (MQ=255)                                                                                                                                                                                                                                        cAGCTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGAACAAGAAATGAGTGAAATGAATTATCAAGTTAAAGTACTGGGCGAAAGCCCGGAGAAAGTGGCGAGGGAGTATTTGAAGAAAATGAAATTGCTATAGCCTTAGACAAACGGTACCgg    >  1:202049/1‑251 (MQ=255)                                                                                                                                                                                                                                           cTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGAACAAGAAATGAGTGAAATGAATTATCAAGTTAAAGTACTGGGCGAAAGCCCGGAGAAAGTGGCGAGGGAGTATTTGAAGAAAATGAAATTGCTATAGCCTTAGACAAACGGTACCgg    <  1:128897/248‑1 (MQ=255)                                                                                                                                                                                                                                           cTCCGCTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGAACAAGAAATGAGTGAAATGAATTATCAAGTTAAAGTACTGGGCGAAAGCCCGGAGAAAGTGGCGAGGGAGTATTTGAAGAAAATGAAATTGCTATAGCCTTAGACAAACGGTACCGGGc  <  1:796126/250‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                      ACACATGGGCTTGCAACAATCAGCGATTTACATCATGTGGCAGGTGACATTCGCGCCGGCTTCACACTGGAATTTCTCGATCGAGTCGATGGCTATAAAGGAATAAAATCACAAGGCATTACTTTAGTTCATATTGTCACCCTTGAACCTGCTTTGCGTTATACGGCGTTACTCAACAATAAAATTGATTTAATCGAAGCATTTTCTACTGACGCAGAACTCAAACAACATCAGCTCCACTTACTGAAAGACGACATTAATTTATTCCCTGCCTATCAAGGTGCGCCACTAATGAAAGCCGATTTTGCAGCAAAAAATCCGCAGGTTCTGACCGCACTTAACCGCCTCGCCAATCGTATTTCCGAACAAGAAATGAGTGAAATGAATTATCAAGTTAAAGTACTGGGCGAAAGCCCGGAGAAAGTGGCGAGGGAGTATTTGAAGAAAATGAAATTGCTATAGCCTTAGACAAACGGTACCGGGC  >  1/1247201‑1247684

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑