Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,241,856	A→G	H441R (CAC→CGC)	unknown →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,241,856	0	A	G	98.9%	223.8 / NA	89	H441R (CAC→CGC)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (1/0);  new base G (41/47);  total (42/47)
Fisher's exact test for biased strand distribution p-value = 4.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

CAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCAAAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCAGTGGAAGAAGAACGTCAACGCGCTATTGAGGCGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGTCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACACCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAATCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCGCTCTACTGCCCCTACGGATTTACCTAGTGATATGGCACCATTAGATCTTGAGGACATTCAAGGAGAAAACAGTGGTGTCGAAAAA  >  1/1241615‑1242100                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     cagGAAGAAAATGCCCGTATAGAACGCGAACGTGCAAAACGTGAAGAAGCCGACCGTCTAGCACGTGAGCAGGAAATTGCCCGTCAAAAAGCAGTGGAAGAAGAACGTCAACGCGCTATTGAGGCGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGTCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAAcgc                                                                                                                                                                                                                                               <  1:461938/249‑1 (MQ=255)   ggAAGAAAATGCCCGTATTGAACGCGAACGTGCAAAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCAGTGGAAGAAGAACGTCAACGCGCTATTGAGGCGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGTCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTa                                                                                                                                                                                                                                           >  1:104693/1‑251 (MQ=255)   ggAAGAAAATGCCCGTATTGAACGCGAACGTGCAAAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCAGTGGAAGAAGAACGCCAACGCGCTATTGAGGCGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCTCAACTACAAGAAGCCGAGCGTCTAGCCCGTGATCAGGAAATTGCCCGTCAACACGCGGTGGAAAAAGAACGCCAACGCGCTa                                                                                                                                                                                                                                           >  1:864493/1‑251 (MQ=11)        gaaacgcagatATTTAACGGGAACGTGTCAAACGTGAAGAAGCCGACCGTCTAGCCCGTGAACAGGAAATTGCCCGTCAAAAAGCAGTGGAAGAAGAACGTCAACGCGCTATTGAGGCGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGTCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTg                                                                                                                                                                                                                                        <  1:232911/239‑1 (MQ=14)           aTGCCCGTATTGAACGCGAACGAGAAAAACGTGAAGAAGACGAACGTCTGGCACGTGAGCAGGAAATTGCCCGTCAAAAAGCAGTGGAAGAAGAACGTCAACGCGCTATTGAGGCGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGTCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACg                                                                                                                                                                                                                                   <  1:670404/251‑1 (MQ=14)              cccGTATTGAACGCGAACGTGCAAAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCAGTGGAAGAAGAACGTCAACGCGCTATTGAGGCGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGTCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCaa                                                                                                                                                                                                                                >  1:413207/1‑251 (MQ=255)              cccGTATTGAACGCGAACGTGCAAAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCAGTGGAAGAAGAACGTCAACGCGCTATTGAGGCGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGTCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCaa                                                                                                                                                                                                                                >  1:411157/1‑251 (MQ=255)                      gAACGCGAACGTGCAAAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCAGTGGAAGAAGAACGTCAACGCGCTATTGAGGCGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGTCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGc                                                                                                                                                                                                                                  <  1:694143/241‑1 (MQ=255)                                          tGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCAGTGGAAGAAGAACGTCAACGCGCTATTGAGGCGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGTCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTg                                                                                                                                                                                                    <  1:272477/251‑1 (MQ=255)                                                agCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCAGTGGAAGAAGAACGTCAACGCGCTATTGAGGCGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGTCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGAGGAAGAAGCACACCAAAGCGCTATTGAAACGCAACGCCAGCAGGAAGAAAATGCCCGCAATGAAcgcg                                                                                                                                                                                              >  1:487335/1‑251 (MQ=255)                                                      ccGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCAGTGGAAGAAGAACGTCAACGCGCTATTGAGGCGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGTCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCTGGAAATTGCCCGTCAAAAAGCAGGGGACGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTg                                                                                                                                                                                        >  1:260092/1‑251 (MQ=255)                                                       cGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCAGTGGAAGAAGAACGTCAACGCGCTATTGAGGCGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGTCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGc                                                                                                                                                                                                                                  <  1:254825/208‑1 (MQ=255)                                                        gTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCAGTGGAAGAAGAACGTCAACGCGCTATTGAGGCGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGTCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCACGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTAAAACGCAACGTCAGCAGGAAGAAAATGACCGAATTGAACGCGAACgagc                                                                                                                                                                                       >  1:341584/1‑247 (MQ=255)                                                        gTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCAGTGGAAGAAGAACGTCAACGCGCTATTGAGGCGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGTCCAACGTGAAGAAGCCGACCGTCTAGCCCGGGAGCAGGAAATTGCCCGTCAAAACGCGGTGGAGGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGc                                                                                                                                                                                       >  1:208741/1‑250 (MQ=255)                                                              cccGTGAGCAGGAAATTGCCCGTCAAAAAGCAGTGGAAGAAGAACGTCAACGCGCTAGTGAGGCGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGTCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACg                                                                                                                                                                                 <  1:516739/250‑1 (MQ=255)                                                                        ggAAATTGCCCGTCAAAAAGCAGTGGAAGAAGAACGTCAACGCGCTATTGAGGCGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGTCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCg                                                                                                                                                                                     <  1:159865/236‑1 (MQ=255)                                                                            aTTGCCCGTCAAAAAGCAGTGGAAGAAGAACGTCAACGCGCTATTGAGGCGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGTCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCg                                                                                                                                                                  <  1:201168/251‑1 (MQ=255)                                                                                   gTCAAAAAGCAGTGGAAGAAGAACGTCAACGCGCTATTGAGGCGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGTCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTgaagaagc                                                                                                                                                                        >  1:618700/1‑236 (MQ=255)                                                                                     cAAAAAGCAGTGGAAGAAGAACGTCAACGCGCTATTGAAGCGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGTCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAACAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCg                                                                                                                                                         <  1:336266/251‑1 (MQ=255)                                                                                              gTGGAAGAAGAACGTCAACGCGCTATTGAGGCGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGTCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGa                                                                                                                                                >  1:681767/1‑251 (MQ=255)                                                                                               tGGAAGAAGAACGTCAACGCGCTATTGAGGCGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGTCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGaa                                                                                                                                               <  1:93304/251‑1 (MQ=255)                                                                                                 gaagaagaaCGTCAACGCGCTATTGAGGCGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGTCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGc                                                                                                                                                                                                                                  <  1:619905/166‑1 (MQ=255)                                                                                                  aagaagaaCGTCAACGCGCTATTGAGGCGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGTCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGcc                                                                                                                                                                                                           <  1:98959/188‑1 (MQ=255)                                                                                                     aagaaCGTCAACGCGCTATTGAGGCGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGTCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGGGAGCAAGAAATTGCa                                                                                                                                         >  1:7742/1‑251 (MQ=255)                                                                                                     aagaaCGTCAACGCGCTATTGAGGCGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGTCCAACGAGAAGAGGCCGACCGTCTAGCGCGTGAGCAGGAAATTGCGCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGcc                                                                                                                                                           <  1:512001/233‑1 (MQ=14)                                                                                                        aaCGTCAACGCGCTATTGAGGCGCGACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCAAAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGc                                                                                                                                       <  1:658653/250‑1 (MQ=255)                                                                                                                    cTATTGAGGCGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGTCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGcc                                                                                                                                                           <  1:705853/218‑1 (MQ=255)                                                                                                                    cTATTGAGGCGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGTCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAg                                                                                                                                                     <  1:749057/224‑1 (MQ=255)                                                                                                                    cTATTGAGGCGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGTCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGc                                                                                                                                          >  1:20833/1‑235 (MQ=255)                                                                                                                    cTATTGAGGCGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGTCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTgaa                                                                                                                          >  1:31783/1‑251 (MQ=255)                                                                                                                    cTATTGAGGCGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGTCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTgaa                                                                                                                          >  1:781384/1‑251 (MQ=255)                                                                                                                    cTATTGAGGCGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGTCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTgaa                                                                                                                          >  1:219572/1‑251 (MQ=255)                                                                                                                       ttGATGCGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAGCGCCAACGTGCAAAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTgaagaa                                                                                                                       <  1:481763/251‑1 (MQ=14)                                                                                                                                          ggAAGAAAATGCCCGTATTGAACGCGAACGTGTCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGcc                                                                                                       >  1:411258/1‑248 (MQ=255)                                                                                                                                                 aaTGCCCGTATTGAACGCGAACGTGTCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCt                                                                                             <  1:742095/251‑1 (MQ=255)                                                                                                                                                   tGCCCGTATTGAACGCGAACGTGTCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGAGGAAGAAGAACGCCAACGCGCTATTGAAAGGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGAAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTg                                                                                           >  1:557248/1‑251 (MQ=255)                                                                                                                                                     cccGTATTGAACGCGAACGTGTCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGcc                                                                                                                                                           <  1:87788/185‑1 (MQ=255)                                                                                                                                                     cccGTATTGAACGCGAACGTGTCAAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAAGTGCTCGTCAAAGAGCGGTGGAAGAAGAGCGTCAGCGCGCTATTGAAGCGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGtt                                                                                         <  1:384761/251‑1 (MQ=17)                                                                                                                                                        gTATTGAACGCGAACGTGTCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCg                                                                                       >  1:759851/1‑250 (MQ=255)                                                                                                                                                        ctagtGAACGCGAACGTGTCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCGc                                                                                      <  1:208423/247‑1 (MQ=255)                                                                                                                                                         tATTGAACGCGAACGTGTCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCg                                                                                       >  1:236219/1‑249 (MQ=255)                                                                                                                                                         tATTGAACGCGAACGTGTCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCg                                                                                       >  1:542527/1‑249 (MQ=255)                                                                                                                                                         tATTGAACGCGAACGTGCAAAACGTGAAGAAGCCGACCGTCTAGCCCGCGAACAGGAAATTGCCCGTCAAAAAGCAGTGGAAGAAGAACGTCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCg                                                                                       >  1:118821/1‑249 (MQ=14)                                                                                                                                                             gAACGCGAACGTGTCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGaaaa                                                                                                                                                                                                               >  1:662133/1‑125 (MQ=255)                                                                                                                                                              aaCGCGAACGTGTCAAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCGCTCTAc                                                                                 <  1:674894/250‑1 (MQ=255)                                                                                                                                                                cgcgAACGTGTACAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAATCGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCGCTCTACTGc                                                                              <  1:517685/251‑1 (MQ=255)                                                                                                                                                                    aaCGTGTCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCGCTCTACTGccc                                                                            >  1:867256/1‑249 (MQ=255)                                                                                                                                                                       gtgtCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCGCTCTACTGCCCCTACg                                                                       >  1:371207/1‑251 (MQ=255)                                                                                                                                                                       gtgcaaaaCGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGc                                                                                                                                                                                       >  1:411860/7‑139 (MQ=255)                                                                                                                                                                        tgtCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGttcttccactgc                                                                                                                                                                                                                  >  1:463371/1‑100 (MQ=17)                                                                                                                                                                        tgtCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCGCTCTACTGCCCCTACg                                                                       <  1:420704/250‑1 (MQ=255)                                                                                                                                                                        tgtCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGCGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCGCTCTACTGCCCCTACgg                                                                      >  1:789947/1‑251 (MQ=255)                                                                                                                                                                          tCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCGCTCTACTGCCCCTACGGa                                                                     <  1:277559/250‑1 (MQ=255)                                                                                                                                                                          caaaaCGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCGCTCTACTGCCCCTACGGa                                                                     <  1:311711/247‑1 (MQ=255)                                                                                                                                                                              aCGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCGCTCTACTGccc                                                                            <  1:786718/239‑1 (MQ=255)                                                                                                                                                                              aCGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCGCTCTACTGCCCCTACGGATTTa                                                                 >  1:75056/1‑250 (MQ=255)                                                                                                                                                                              aCGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCGCTCTACTGCCCCTACGGATTTa                                                                 >  1:824604/1‑250 (MQ=255)                                                                                                                                                                              aCGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCGAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCGCTCTACTGCCCCTACGGATTTa                                                                 >  1:79746/1‑250 (MQ=255)                                                                                                                                                                                gTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCGCTCTACTGCCCCTACGGATTTAc                                                                >  1:74972/1‑249 (MQ=255)                                                                                                                                                                                 tGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCGCTCTACTGCCCCTACGGa                                                                     >  1:682058/1‑243 (MQ=255)                                                                                                                                                                                 tGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCGCTCTACTGCCCCTACGGATTTACCt                                                              <  1:156453/250‑1 (MQ=255)                                                                                                                                                                                 tGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCGCTCTACTGCCCCTACGGATTTACCTa                                                             >  1:456905/1‑251 (MQ=255)                                                                                                                                                                                 tGAAGAAGACGAATGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAGCGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCGCTCTACTGCCCCTACGGATTTACCt                                                              <  1:112857/250‑1 (MQ=255)                                                                                                                                                                                        gCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGAATTGAACGCGAACGTGCCGAACGTGACGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCtt                                                                                            >  1:70883/1‑213 (MQ=255)                                                                                                                                                                                            aCCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGcc                                                                                                                                                           >  1:584917/1‑146 (MQ=255)                                                                                                                                                                                            aCCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCGCTCTACTGCCCCTACGGATTTACCTAGTGATATGGc                                                   >  1:74072/1‑250 (MQ=255)                                                                                                                                                                                             ccGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAGCGCCAACGCGCTATTAAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCGCTCTACTGCCCCTACGGATTTACCTAGTGATATGGCAc                                                 <  1:834042/251‑1 (MQ=255)                                                                                                                                                                                             ccGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCGCTCTACTGCCCCTACGGATTTACCTAGTGATATGGCAc                                                 <  1:643128/251‑1 (MQ=255)                                                                                                                                                                                                     cccGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCGCTCTACTGCCCCTACGGATTTACCTAGTGATATGGCACCATTAGAt                                         <  1:174024/251‑1 (MQ=255)                                                                                                                                                                                                     cccGTGAGCAGGAAATTGCCCGTCAAAAAGCAGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCGCTCTACTGCCCCTACGGATTTACCTAGTGATATGGCACCATTAGAt                                         <  1:514678/251‑1 (MQ=255)                                                                                                                                                                                                      ccGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCGCTCTACTGCCCCTACGGATTTACCTAGTGATATGGCACCATTAGATc                                        <  1:139730/251‑1 (MQ=255)                                                                                                                                                                                                      ccGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCGCTCTACTGCCCCTACGGATTTACCTAGTGATATGGCACCATTAGATc                                        <  1:151720/251‑1 (MQ=255)                                                                                                                                                                                                        gTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCGCTCTACTGCCCCTACGGATTTACCTAGTGATATGGCACCATTAGAt                                         <  1:352063/248‑1 (MQ=255)                                                                                                                                                                                                            gCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGt                                                                                                                                                                 >  1:820907/1‑124 (MQ=255)                                                                                                                                                                                                               ggAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTc                                                                                              <  1:766231/188‑1 (MQ=255)                                                                                                                                                                                                               ggAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCGCTCTACTGCCCCTACGGATTTACCTAGTGATATgg                                                    >  1:128069/1‑230 (MQ=255)                                                                                                                                                                                                                 aaaTTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCGCTCTACTGCCCCTACGGATTTACCTAGTGATATGGCACCATTAGAt                                         <  1:304213/239‑1 (MQ=255)                                                                                                                                                                                                                 aaaTTGCCCGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCGCTCTACTGCCCCTACGGATTTACCTAGTGATATGGCACCATTAGATCTTGAgg                                  <  1:12215/246‑1 (MQ=255)                                                                                                                                                                                                                       cccGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCGCTCTACTGCCCCTACGGATTTACCTAGTGATATGGCACCATTAGATCTTGAGGACATTCAAgg                        <  1:114041/250‑1 (MQ=255)                                                                                                                                                                                                                        ccGTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAGCGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCGCTCTACTGCCCCTACGGATTTACCTAGTGATATGGCACCATTAGATCTTGAGGACATTCAAGgag                      <  1:848430/251‑1 (MQ=255)                                                                                                                                                                                                                          gTCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCGCTCTACTGCCCCTACGGATTTACCTAGTGATATGGCACCATTAGATCTTGAGGACATTCAAGGAGaa                    >  1:463472/1‑251 (MQ=255)                                                                                                                                                                                                                           tCAAAAAGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCGCTCTACTGCCCCTACGGATTTACCTAGTGATATGGCAc                                                 >  1:718286/1‑221 (MQ=255)                                                                                                                                                                                                                               aaaGCGGTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCGCTCTACTGCCCCTACGGATTTACCTAGTGATATGGCACCATTAGATCTTGAGGACATTCAAGGAGAAAACAg               <  1:381218/251‑1 (MQ=255)                                                                                                                                                                                                                                     gTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCGCTCTACTGCCCCTACGGATTTACCTAGTGATATGGCACCATTAGAt                                         >  1:786950/1‑219 (MQ=255)                                                                                                                                                                                                                                     gTGGAAGAAGAACGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCGCTCTACTGCCCCTACGGATTTACCTAGTGATATGGCACCATTAGATCTTGAgg                                  >  1:34238/1‑226 (MQ=255)                                                                                                                                                                                                                                        gaagaagaaCGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCGCTCTACTGCCCCTACGGATTTACCTAGTGat                                                        <  1:862485/201‑1 (MQ=255)                                                                                                                                                                                                                                        gaagaagaaCGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCGCTCTACTGCCCCTACGGATTTACCTAGTGATATGGCACCATTAGATCTTGAgg                                  <  1:617723/223‑1 (MQ=255)                                                                                                                                                                                                                                            aagaaCGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCGCTCTACTGCCCCTACGGATTTACCTAGTGATATGGCACCATTAGATCTTGAGGACATTCAAGGAGAAAACAGTGGTGTCGaaaaa  <  1:241884/251‑1 (MQ=255)                                                                                                                                                                                                                                            aagaaCGCCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCGCTCTACTGCCCCTACGGATTTACCTAGTGATATGGCACCATTAGATCTTGAGGACATTCAAGGAGAAAACAGTGGTGTCGaaaaa  <  1:666987/251‑1 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     CAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCAAAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCAGTGGAAGAAGAACGTCAACGCGCTATTGAGGCGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGTCCAACGTGAAGAAGCCGACCGTCTAGCCCGTGAGCAGGAAATTGCCCGTCAAAAAGCGGTGGAAGAAGAACACCAACGCGCTATTGAAACGCAACGTCAGCAGGAAGAAAATGCCCGTATTGAACGCGAACGTGCCGAACGTGAAGAATCCGACCGTCTAGCCCGTGAGCAAGAAATTGCAGCTCAACAAGTTGAAGAAAAACCGGAAGAAAGCCCTGTTATTCTTGTTCGCTCTACTGCCCCTACGGATTTACCTAGTGATATGGCACCATTAGATCTTGAGGACATTCAAGGAGAAAACAGTGGTGTCGAAAAA  >  1/1241615‑1242100

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑