Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,240,798	A→G	E88E (GAA→GAG)	unknown →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,240,798	0	A	G	100.0%	172.7 / NA	60	E88E (GAA→GAG)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (32/28);  total (32/28)

ATTCTGTTATAGCAACAACCATCGCATTGGTATTTAATCAACCGGCATTTGCGTCAGAGTTTAATGCTCAAATAAATAATCAACCGTCTTCTCAGGCTAAAACAAAAAGTAAAACCAATATTAAAAAAAATATGCAGAACAACATATCTAATTCTGCAAAAGAATCCGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAAATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGAATTAGAACAGAAAAAGGCGGAACTTGTTCAACGTGAGTATTTAGAAGCTAAGCGTCAAGTCCGTGAACAAGAAATGGCACGCCAGAAGGCAGAAGAAGATCGTAAAGTAGCAGAGGCACTTGCGGCCGTCAAACTAAAGAATTCCACCCAACAACGTTTAACT  >  1/1240563‑1241041                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                    aTTCTGTTATAGCAACAACCATCGCATTGGTATTTAATCAACCGGCATTTGCGTCAGAGTTTAATGCTCAAATAAATAATCAACCGTCTTCTCAGGCTAAAACAAAAAGTAAAACCAATATTAAAAAAAATATGCAGAACAACATATCTAATTCTGCAAAAGAATCCGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAaagaa                                                                                                                                                                                                                                      >  1:677432/1‑251 (MQ=255) aTTCTGTTATAGCAACAACCATCGCATTGGTATTTAATCAACCGGCATTTGCGTCAGAGTTTAATGCTCAAATAAATAATCAACCGTCTTCTCAGGCTAAAACAAAAAGTAAAACCAATATTAAAAAAAATATGCAGAACAACATATCTAATTCTGCAAAAGAATCCGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAaagaa                                                                                                                                                                                                                                      >  1:400855/1‑251 (MQ=255)            gCAACAACCATCGCATTGGTATTTAATCAACCGGCATTTGCGTCAGAGTTTAATGCTCAAATAAATAATCAACCGTCTTCTCAGGCTAAAACAAAAAGTAAAACCAATATTAAAAAAAATATGCAGAACAACATATCTAATTCTGCAAAAGAATCCGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCg                                                                                                                                                                                                                           >  1:171923/1‑251 (MQ=255)                caacCATCGCATTGGTATTTAATCAACCGGCATTTGCGTCAGAGTTTAATGCTCAAATAAATAATCAACCGTCTTCTCAGGCTAAAACAAAAAGTAAAACCAATATTAAAAAAAATATGCAGAACAACATATCTAATTCTGCAAAAGAATCCGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCa                                                                                                                                                                                                                       <  1:352352/251‑1 (MQ=255)                   ccATCGCATTGGTATTTAATCAACCGGCATTTGCGTCAGAGTTTAATGCTCAAATAAATAATCAACCGTCTTCTCAGGCTAAAACAAAAAGTAAAACCAATATTAAAAAAAATATGCAGAACAACATATCTAATTCTGCAAAAGAATCCGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATa                                                                                                                                                                                                                    >  1:52817/1‑251 (MQ=255)                       cGCATTGGTATTTAATCAACCGGCATTTGCGTCAGAGTTTAATGCTCAAATAAATAATCAACCGTCTTCTCAGGCTAAAACAAAAAGTAAAACCAATATTAAAAAAAATATGCAGAACAACATATCTAATTCTGCAAAAGAATCCGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGcac                                                                                                                                                                                                                <  1:103884/251‑1 (MQ=255)                       cGCATTGGTATTTAATCAACCGGCATTTGCGTCAGAGTTTAATGCTCAAATAAATAATCAACCGTCTTCTCAGGCTAAAACAAAAAGTAAAACCAATATTAAAAAAAATATGCAGAACAACATATCTAATTCTGCAAAAGAATCCGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGcac                                                                                                                                                                                                                <  1:173356/251‑1 (MQ=255)                             ggTATTTAATCAACCGGCATTTGCGTCAGAGTTTAATGCTCAAATAAATAATCAACCGTCTTCTCAGGCTAAAACAAAAAGTAAAACCAATATTAAAAAAAATATGCAGAACAACATATCTAATTCTGCAAAAGAATCCGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGcc                                                                                                                                                                                                                             >  1:260378/1‑232 (MQ=255)                                       cAACCGGCATTTGCGTCAGAGTTTAATGCTCAAATAAATAATCAACCGTCTTCTCAGGCTAAAACAAAAAGTAAAACCAATATTAAAAAAAATATGCAGAACAACATATCTAATTCTGCAAAAGAATCCGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGcc                                                                                                                                                                                                                             >  1:647420/1‑222 (MQ=255)                                                         gagTTTAATGCTCAAATAAATAATCAACCGTCTTCTCAGGCTAAAACAAAAAGTAAAACCAATATTAAAAAAAATATGCAGAACAACATATCTAATTCTGCAAAAGAATCCGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAag                                                                                                                                                                                                       <  1:78908/226‑1 (MQ=255)                                                           gTTTAATGCTCAAATAAATAATCAACCGTCTTCTCAGGCTAAAACAAAAAGTAAAACCAATATTAAAAAAAATATGCAGAACAACATATCTAATTCTGCAAAAGAATCCGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAg                                                                                                                                                                            >  1:371759/1‑251 (MQ=255)                                                           gTTTAATGCTCAAATAAATAATCAACCGTCTTCTCAGGCTAAAACAAAAAGTAAAACCAATATTAAAAAAAATATGCAGAACAACATATCT‑ATTCTGCAAAAGAATCCGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGcac                                                                                                                                                                                                                >  1:164799/1‑214 (MQ=255)                                                                     cAAATAAATAATCAACCGTCTTCTCAGGCTAAAACAAAAAGTAAAACCAATATTAAAAAAAATATGCAGAACAACATATCTAATTCTGCAAAAGAATCCGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGAAtt                                                                                                                                                                  >  1:9832/1‑251 (MQ=255)                                                                       aataaataATCAACCGTCTTCTCAGGCTAAAACAAAAAGTAAAACCAATATTAAAAAAAATATGCAGAACAACATATCTAATTCTGCAAAAGAATCCGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGAATTAg                                                                                                                                                                >  1:269077/1‑251 (MQ=255)                                                                          aaataaTCAACCGTCTTCTCAGGCTAAAACAAAAAGTAAAACCAATATTAAAAAAAATATGCAGAACAACATATCTAATTCTGCAAAAGAATCCGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGAATTAGAAc                                                                                                                                                             <  1:489608/251‑1 (MQ=255)                                                                                    ccGTCTTCTCAGGCTAAAACAAAAAGTAAAACCAATATTAAAAAAAATATGCAGAACAACATATCTAATTCTGCAAAAGAATCCGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGAATTAGAACAGAAAAAGGc                                                                                                                                                   <  1:40537/251‑1 (MQ=255)                                                                                      gTCTTCTCAGGCTAAAACAAAAAGTAAAACCAATATTCAAAAAAATATGCAGAACAACATATCTAATTCTGCAAAAGAATCCGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAACAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGAATTAGAACAGAAAAAGGCgg                                                                                                                                                 >  1:210787/1‑251 (MQ=255)                                                                                          tctcAGGCTAAAACAAAAAGTAAAACCAATATTAAAAAAAATATGCAGAACAACATATCTAATTCTGCAAAAGAATCCGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAg                                                                                                                                                                                                                   <  1:872805/181‑1 (MQ=255)                                                                                                   aaaaCAAAAAGTAAAACCAATATTAAAAAAAATATGCAGAACAACATATCTAATTCTGCAAAAGAATCCGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGAATTAGAATAGAAAAAGGCGGAACTTGTTCAACg                                                                                                                                    <  1:276516/251‑1 (MQ=255)                                                                                                   aaaaCAAAAAGTAAAACCAATATTAAAAAAAATATGCAGAACAACATATCTAATTCTGCAAAAGAATCCGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGAATTAGAACAGAAAAAGGCGGAACTTGTTCAACg                                                                                                                                    <  1:533230/251‑1 (MQ=255)                                                                                                                 aaCCAATATTAAAAAAAATATGCAGAACAACATATCTAATTCTGCAAAAGAATCCGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGAATTAGAACAGAAAAAGGCGGAACTTGTTCAACGTGAGTATTTAGAAg                                                                                                                      <  1:57501/251‑1 (MQ=255)                                                                                                                  aCCAATATTAAAAAAAATATGCAGAACAACATATCTAATTCTGCAAAAGAATCCGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGAATTAGAACAGAAAAAGGCGGAACTTGTTCAACGTGAGTATTTAGAAGc                                                                                                                     <  1:552422/251‑1 (MQ=255)                                                                                                                  aCCAATATTAAAAAAAATATGCAGAACAACATATCTAATTCTGCAAAAGAATCCGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGAATTAGAACAGAAAAAGGCGGAACTTGTTCAACGTGAGTATTTAGAAGc                                                                                                                     <  1:286884/251‑1 (MQ=255)                                                                                                                               aaaaTATGCAGAACAACATATCTAATTCTGCAAAAGAATCCGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGAATTAGAACAGAAAAAGGCGGAACTTGTTCAACGTGAGTATTTAGAAGCTAAGCGTCAAGTc                                                                                                        <  1:676132/251‑1 (MQ=255)                                                                                                                               aaaaTATGCAGAACAACATATCTAATTCTGCAAAAGAATCCGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGAATTAGAACAGAAAAAGGCGGAACTTGTTCAACGTGAGTATTTAGAAGCTAAGCGTCAAGTc                                                                                                        <  1:12837/251‑1 (MQ=255)                                                                                                                                    atGCAGAACAACATATCTAATTCTGCAAAAGAATCCGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGAATTAGAACAGAAAAAGGCGGAACTTGTTCAACGTGAGTATTTAGAAGCTAAGCGTCAAGTCCGTGa                                                                                                   >  1:836764/1‑251 (MQ=255)                                                                                                                                         gAACAACATATCTAATTCTGCAAAAGAATCCGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAagag                                                                                                                                                                                                     <  1:204330/148‑1 (MQ=255)                                                                                                                                              acaTATCTAATTCTGCAAAAGAATCCGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGAATTAGAACAGAAAAAGGCGGAACTTGTTCAACg                                                                                                                                    >  1:696032/1‑208 (MQ=255)                                                                                                                                              acaTATCTAATTCTGCAAAAGAATCCGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGAATTAGAACAGAAAAAGGCGGAACTTGTTCAACGTGAGTATTTAGAAGCTAAGCGTCAAGTCCGTGAACAAGAAATg                                                                                         >  1:698110/1‑251 (MQ=255)                                                                                                                                               caTATCTAATTCTGCAAAAGAATCCGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGAATTAGAACAGAAAAAGGCGGAACTTGTTCAACGTGAGTATTTAGAAGCTAAGCGTCAAGTCCGTGAACAAGAAATgg                                                                                        >  1:467460/1‑251 (MQ=255)                                                                                                                                                atatCTAATTCTGCAAAAGAATCCGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGAATTAGAACAGAAAAAGGCGGAACTTGTTCAACGTGAGTATTTAGAAGCTAAGCGTCAAGTCCGTGAACAAGAAATgg                                                                                        >  1:337724/1‑250 (MQ=255)                                                                                                                                                 tatCTAATTCTGCAAAAGAATCCGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGc                                                                                                                                                                           <  1:32814/166‑1 (MQ=255)                                                                                                                                                  atCTAATTCTGCAAAAGAATCCGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGcac                                                                                                                                                                                                                >  1:224736/1‑128 (MQ=255)                                                                                                                                                  atCTAATTCTGCAAAAGAATCCGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGaa                                                                                                                                                                    >  1:559766/1‑172 (MQ=255)                                                                                                                                                  atCTAATTCTGCAAAAGAATCCGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGAATTAGAAc                                                                                                                                                             >  1:796841/1‑179 (MQ=255)                                                                                                                                                  atCTAATTCTGCAAAAGAATCCGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGAATTAGAACAGAAAAAGGCGGAACTTGTTCAACGTGAGTATTTAGAAGCTAAGCGTCAAGTCCGTGAACAAGAAATGGCa                                                                                      >  1:262397/1‑250 (MQ=255)                                                                                                                                                  atCTAATTCTGCAAAAGAATCCGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGAATTAGAACAGAAAAAGGCGGAACTTGTTCAACGTGAGTATTTAGAAGCTAAGCGTCAAGTCCGTGAACAAGAAATGGCa                                                                                      >  1:662897/1‑250 (MQ=255)                                                                                                                                                          cTGCAAAAGAATCCGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGcac                                                                                                                                                                                                                                              >  1:360973/1‑90 (MQ=255)                                                                                                                                                          cTGCAAAAGAATCCGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGAATTAGAACAGAAAAAGGCGGAACTTGTTCAACGTGAGTATTTAGAAGCTAAGCGTCAAGTCCGTGAACAAGAAATGGCACGCCAGAAg                                                                             <  1:318019/251‑1 (MQ=255)                                                                                                                                                                      ccGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGaa                                                                                                                                                                    >  1:800952/1‑152 (MQ=255)                                                                                                                                                                      ccGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGAATTAGAACAGAAAAAGGc                                                                                                                                                   <  1:231285/169‑1 (MQ=255)                                                                                                                                                                      ccGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGAATTAGAACAGAAAAAGGCGGAACTTGTTCAACGTGAGTa                                                                                                                              <  1:571047/190‑1 (MQ=255)                                                                                                                                                                      ccGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGAATTAGAACAGAAAAAGGCGGAACTTGTTCAACGTGAGTATTTAGAAGCTAAGCGTCAAGTCCGTGAACAAGAAATGGCACGCCAGAAGGCAGAAGAAGAt                                                                 >  1:195362/1‑251 (MQ=255)                                                                                                                                                                       cGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGAATTAGAACAGAAAAAGGCGGAACTTGTTCAACGTGAGTATTTAGAAGCTAAGCGTCAAGTCCGTGAACAAGAAATGGCACGCCAGAAGGCAGAAGAAGAt                                                                 >  1:386909/1‑250 (MQ=255)                                                                                                                                                                        gTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGAATTAGAACAGAAAAAGGc                                                                                                                                                   <  1:651683/167‑1 (MQ=255)                                                                                                                                                                            aTACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGAATTAGAACAGAAAAAGGCGGAACTTGTTCAACGt                                                                                                                                   >  1:706998/1‑179 (MQ=255)                                                                                                                                                                              aCTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGAATTAGAACAGAAAAAGGCGGAACTTGTTCAACGTGAGTATTTAGAAGCTAAGCGTCAAGTCCGTGAACAAGAAATGGCACGCCAGAAGGCAGAAGAAGATCGTAAAGt                                                         <  1:539024/251‑1 (MQ=255)                                                                                                                                                                                 tATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGAATTAGAACAGAAAAAGGCGGAACTTGTTCAACGTGAGTATTTAGAAGCTAAGCGTCAAGTCCGTGAACAAGAAATGGCACGCCAGAAGGCAGAAGAAGATCGTAAAGTAg                                                       >  1:399916/1‑250 (MQ=255)                                                                                                                                                                                                  gCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGAATTAGAACAGAAAAAGGCGGAACTTGTTCAACGTGAGTATTTAGAAGCTAAGCGTCAAGTCCGTGATCAAGAAATGGCACGCCAGAAGGCAGAAGAAGATCGTAAAGTAGCAGAGGCACTTGCGGCCg                                     >  1:643640/1‑251 (MQ=255)                                                                                                                                                                                                     tATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGc                                                                                                                                                                                                                        <  1:599764/69‑1 (MQ=255)                                                                                                                                                                                                     tATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGAATTAGAACAGAAAAAGGCGGAACTTGTTCAACGTGAGTATTTAGAAGCTAAGCGTCAAGTCCGTGAACAAGAAATGGc                                                                                       <  1:885921/198‑1 (MQ=255)                                                                                                                                                                                                         aCTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGAATTAGAACAGAAAAAGGCGGAACTTGTTCAACGTGAGTATTTAGAAGCTAAGCGTCAAGTCCGTGAACAAGAAATGGCACGCCAGAAGGCAGAAGAAGATCGTAAAGTAGCAGAGGCACTTGCGGCCGTCAAACt                              <  1:443879/251‑1 (MQ=255)                                                                                                                                                                                                              cGCACAAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGAATTAGAACAGAAAAAGGCGGAACTTGTTCAACGTGAGTATTTAGAAGCTAAGCGTCAAGTCCGTGAACAAGAAATGGCACGCTAGAAGGCAGAAGAAGATCGTAAAGTAGCAGAGGCACTTGCGGCCGTCAAACTAAAGa                         <  1:108252/251‑1 (MQ=255)                                                                                                                                                                                                                cacaAAAACTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGAATTAGAACAGAAAAAGGCGGAACTTGTTCAACGTGAGTATTTAGAAGCTAAGCGTCAAGTCCGTGAACAAGAAATGGCACGCCAGAAGGCAGAAGAAGATCGTAAAGTAGCAGAGGCACTTGCGGCCGTCAAACTAAAGAAt                       >  1:483157/1‑251 (MQ=255)                                                                                                                                                                                                                      aaCTTGCCCGTCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGAATTAGAACAGAAAAAGGCGGAACTTGTTCAACGTGAGTATTTAGAAGCTAAGCGTCAAGTCCGTGAACAAGAAATGGCACGCCAGAAGGCAGAAGAAGAt                                                                 <  1:859183/203‑1 (MQ=255)                                                                                                                                                                                                                                tCAAAAAGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGAATTAGAACAGAAAAAGGCGGAACTTGTTCAACGTGAGTATTTAGAAGCTAAGCGTCAAGTCCGTGAACAAGAAATGGCACGCCAGAAGGCAGAAGAAGATCGTAAAGTAGCAGAGGCACTTGCGGCCGTCAAACTAAAGAATTCCACCCAACAACGtt       >  1:771723/1‑251 (MQ=255)                                                                                                                                                                                                                                     aaGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGAATTAGAACAGAAAAAGGCGGAACTTGTTCAACGTGAGTAt                                                                                                                             >  1:140777/1‑128 (MQ=255)                                                                                                                                                                                                                                     aaGCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGAATTAGAACAGAAAAAGGCGGAACTTGTTCAACGTGAGTATTTAGAAGCTAAGCGTCAAGTCCGTGAACAAGAAATGGCACGCCAGAAGGCAGAAGAAGATCGTAAAGTAGCAGAGGCACTTGCGGCCGTCAAACTAAAGAATTCCACCCAACAACGTTTAACt  <  1:30440/251‑1 (MQ=255)                                                                                                                                                                                                                                     aaGCTGAGATGGCACAAAAAAAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGAATTAGAACAGAAAAAGGCGGAACTTGTTCAACGTGAGTATTTAGAAGCTAAGCGTCAAGTCCGTGAACAAGAAATGGCACGCCAGAAGGCAGAAGAAGATCGTAAAGTAGCAGAGGCACTTGCGGCCGTCAAACTAAAGAATTCCACCCAACAACGTTTAACt  <  1:731955/251‑1 (MQ=255)                                                                                                                                                                                                                                       gCTGAGATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGAATTAGAACAGAAAAAGGCGGAACTTGTTCAACGTGAGTATTTAGAAGCTAAGCGTCAAGTCCGTGAACAAGAAATGGCACGCCAGAAGGCAGAAGAAGATCGTAAAGTAGCAGAGGCACTTGCGGCCGTCAAACTAAAGa                         >  1:677983/1‑226 (MQ=255)                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                    ATTCTGTTATAGCAACAACCATCGCATTGGTATTTAATCAACCGGCATTTGCGTCAGAGTTTAATGCTCAAATAAATAATCAACCGTCTTCTCAGGCTAAAACAAAAAGTAAAACCAATATTAAAAAAAATATGCAGAACAACATATCTAATTCTGCAAAAGAATCCGTTAATACTTATCAAACGGAAATTCAGCGTATCACTGCCGCACAAAAACTTGCCCGTCAAAAAGCTGAAATGGCACAAAAAGAAGCGGAAGCCCGTGCAATAGCACAAGTAAAAGAGAAACGTCAACAAAGCCAAATTAAAGCACAAGAATTAGAACAGAAAAAGGCGGAACTTGTTCAACGTGAGTATTTAGAAGCTAAGCGTCAAGTCCGTGAACAAGAAATGGCACGCCAGAAGGCAGAAGAAGATCGTAAAGTAGCAGAGGCACTTGCGGCCGTCAAACTAAAGAATTCCACCCAACAACGTTTAACT  >  1/1240563‑1241041

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑