Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,231,450	T→C	Y48C (TAT→TGT)	unknown ←	UPF0381 protein YfcZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,231,450	0	T	C	100.0%	146.4 / NA	51	Y48C (TAT→TGT)	unknown	UPF0381 protein YfcZ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (26/25);  total (26/25)

GTTAATTTCATCGTAAATAGAATACATCGAGTTATAATAAAAAAGGCTTAAGTTTTCGACTTAAGCCTTTTTTATTTATATCAATCAATTAACGGGTAGAAAGTTGGAAAATCATGGATTCCGCCTGGCAGCTAAATTCAAATTTGGCAGTTAATTTGAAGCCATTTTCCACCACACTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAATAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAACCTGTTCAAAATCCACCGTGCAGTCAGAACCGTCAATCACCGTACCCACCTCGACACAACAGCAAACTTTTGCTTCTTCCGCCTGACATTTCAATGTGCTCATAATATCTCCTTGTAAAAAGAATTGAAATTTTCAACTATTTTAGCATTATTCAAATTTAATACAAAATCCGACCGCACTTTTCTTTGTCCACTGGTCTGAACAC  >  1/1231214‑1231695                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      gTTAATTTCATCGTAAATAGAATACATCGAGTTATAATAAAAAAGGCTTAAGTTTTCGACTTAAGCCTTTTTTATTTATATCAATCAATTAACGGGTAGAAAGTTGGAAAATCATGGATTCCGCCTGGCAGCTAAATTCAAATTTGGCAGTTAATTTGAAGCCATTTTCCACCACACTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGctt                                                                                                                                                                                                                                           >  1:680674/1‑249 (MQ=255)               aaaTAGAATACATCGAGTTATAATAAAAAAGGCTTAAGTTTTCGACTTAAGCCTTTTTTATTTATATCAATCAATTAACGGGTAGAAAGTTGGAAAATCATGGATTCCGCCTGGCAGCTAAATTCAAATTTGGCAGTTAATTTGAAGCCATTTTCCACCACACTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGcttctt                                                                                                                                                                                                                            >  1:270902/1‑250 (MQ=255)                 aTAGAATACATCGAGTTATAATAAAAAAGGCTTAAGTTTTCGACTTAAGCCTTTTTTATTTATATCAATCAATTAACGGGTAGAAAGTTGGAAAATCATGGATTCCGCCTGGCAGCTAAATTCAAATTTGGCAGTTAATTTGAAGCCATTTTCCACCACACTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGc                                                                                                                                                                                                                                             >  1:675200/1‑231 (MQ=255)                   aGAATACATCGAGTTATAATAAAAAAGGCTTAAGTTTTCGACTTAAGCCTTTTTTATTTATATCAATCAATTAACGGGTAGAAAGTTGGAAAATCATGGATTCCGCCTGGCAGCTAAATTCAAATTTGGCAGTTAATTTGAAGCCATTTTCCACCACACTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGtt                                                                                                                                                                                                                       >  1:682748/1‑251 (MQ=255)                    gAATACATCGAGTTATAATAAAAAAGGCTTAAGTTTTCGACTTAAGCCTTTTTTATTTATATCAATCAATTAACGGGTAGAAAGTTGGAAAATCATGGATTCCGCCTGGCAGCTAAATTCAAATTTGGCAGTTAATTTGAAGCCATTTTCCACCACACTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGc                                                                                                                                                                                                                                             <  1:808920/228‑1 (MQ=255)                    gAATACATCGAGTTATAATAAAAAAGGCTTAAGTTTTCGACTTAAGCCTTTTTTATTTATATCAATCAATTAACGGGTAGAAAGTTGGAAAATCATGGATTCCGCCTGGCAGCTAAATTCAAATTTGGCAGTTAATTTGAAGCCATTTTCCACCACACTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTg                                                                                                                                                                                                                      >  1:27873/1‑251 (MQ=255)                                     ataaAAAAGGCTTAAGTTTTCGACTTAAGCCTTTTTTATTTATATCAATCAATTAACGGGTAGAAAGTTGGAAAATCATGGATTCCGCCTGGCAGCTAAATTCAAATTTGGCAGTTAATTTGAAGCCATTTTCCACCACACTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAACCTGTTCaaaa                                                                                                                                                                                                     >  1:638453/1‑251 (MQ=255)                                             ggCTTAAGTTTTCGACTTAAGCCTTTTTTATTTATATCAATCAATTAACGGGTAGAAAGTTGGAAAATCATGGATTCCGCCTGGCAGCTAAATTCAAATTTGGCAGTTAATTTGAAGCCATTTTCCACCACACTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAAcc                                                                                                                                                                                                              <  1:900773/234‑1 (MQ=255)                                                               aaGCCTTTTTTATTTATATCAATCAATTAACGGGTAGAAAGTTGGAAAATCATGGATTCCGCCTGGCAGCTAAATTCAAATTTGGCAGTTAATTTGAAGCCATTTTCCACCACACTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAAcc                                                                                                                                                                                                              <  1:854292/216‑1 (MQ=255)                                                                          atttatATCAATCAATTAACGGGTAGAAAGTTGGAAAATCATGGATTCCGCCTGGCAGCTAAATTCAAATTTGGCAGTTAATTTGAAGCCATTTTCCACCACACTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTcc                                                                                                                                                                                                                          <  1:521788/193‑1 (MQ=255)                                                                          atttatATCAATCAATTAACGGGTAGAAAGTTGGAAAATCATGGATTCCGCCTGGCAGCTAAATTCAAATTTGGCAGTTAATTTGAAGCCATTTTCCACCACACTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCg                                                                                                                                                                                                                         >  1:692053/1‑194 (MQ=255)                                                                                 tcaatcaaTTAACGGGTAGAAAGTTGGAAAATCATGGATTCCGCCTGGCAGCTAAATTCAAATTTGGCAGTTAATTTGAAGCCATTTTCCACCACACTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAACCTGTTCAAAATCCACCGTGCAGTCAGAACCGTCAATCACCGTACCCACCTCGac                                                                                                                                                         <  1:634574/251‑1 (MQ=255)                                                                                   aatcaatTAACGGGTAGAAAGTTGGAAAATCATGGATTCCGCCTGGCAGCTAAATTCAAATTTGGCAGTTAATTTGAAGCCATTTTCCACCACACTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAACCTGTTCAAAATCCACCGTGCAGTCAGAACCGTCAATCACCGTACCCACCTCGacac                                                                                                                                                       <  1:62256/251‑1 (MQ=255)                                                                                   aatcaatTAACGGGTAGAAAGTTGGAAAATCATGGATTCCGCCTGGCAGCTAAACTCAAATTTGGCAGTTAATTTGAAGCCATTTTCCACCACACTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAACCTGTTCAAAATCCACCGTGCAGTCAGAACCGTCAATCACCGTACCCa                                                                                                                                                                >  1:306269/1‑242 (MQ=255)                                                                                    atcaatTAACGGGTAGAAAGTTGGAAAATCATGGATTCCGCCTGGCAGCTAAATTCAAATTTGGCAGTTAATTTGAAGCCATTTTCCACCACACTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAAcc                                                                                                                                                                                                              >  1:485066/1‑195 (MQ=255)                                                                                    atcaatTAACGGGTAGAAAGTTGGAAAATCATGGATTCCGCCTGGCAGCTAAATTCAAATTTGGCAGTTAATTTGAAGCCATTTTCCACCACACTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAACCTGTTCAAAATCCACCGTGCAGTCAGAACCGTCAATCACCGTACCCACCTCGAcaca                                                                                                                                                      >  1:251870/1‑251 (MQ=255)                                                                                      caatTAACGGGTAGAAAGTTGGAAAATCATGGATTCCGCCTGGCAGCTAAATTCAAATTTGGCAGTTAATTTGAAGCCATTTTCCACCACACTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTTCGTTGCATAAACCTGTTCAAAATCCACCGTGCAGTCAGAACCGTCAATCACCGTACCCACCTCGACacaa                                                                                                                                                     >  1:609798/1‑250 (MQ=255)                                                                                      caatTAACGGGTAGAAAGTTGGAAAATCATGGATTCCGCCTGGCAGCTAAATTCAAATTTGGCAGTTAATTTGAAGCCATTTTCCACCACACTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAACCTGTTc                                                                                                                                                                                                         <  1:161676/198‑1 (MQ=255)                                                                                        atTAACGGGTAGAAAGTTGGAAAATCATGGATTCCGCCTGGCAGCTAAATTCAAATTTGGCAGTTAATTTGAAGCCATTTTCCACCACACTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAACCTGTTCAAAAt                                                                                                                                                                                                    <  1:778607/201‑1 (MQ=255)                                                                                        atTAACGGGTAGAAAGTTGGAAAATCATGGATTCCGCCTGGCAGCTAAATTCAAATTTGGCAGTTAATTTGAAGCCATTTTCCACCACACTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAACCTGTTCAAAATCCACCGTGCAGTCAGAACCGTCAATCACCGTACCCACCTCGACACAACAg                                                                                                                                                  >  1:762466/1‑251 (MQ=255)                                                                                                gTAGAAAGTTGGAAAATCATGGATTCCGCCTGGCAGCTAAATTCAAATTTGGCAGTTAATTTGAAGCCATTTTCCACCACACTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAAcc                                                                                                                                                                                                              >  1:281819/1‑183 (MQ=255)                                                                                                                    ggATTCCGCCTGGCAGCTAAATTCAAATTTGGCAGTTAATTTGAAGCCATTTTCCACCACACTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAAcc                                                                                                                                                                                                              >  1:120570/1‑163 (MQ=255)                                                                                                                    ggATTCCGCCTGGCAGCTAAATTCAAATTTGGCAGTTAATTTGAAGCCATTTTCCACCACACTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAACCTGTTCAAAATCCACCGTGCAGTCAGAAc                                                                                                                                                                                  <  1:711315/191‑1 (MQ=255)                                                                                                                         ccGCCTGGCAGCTAAATTCAAATTTGGCAGTTAATTTGAAGCCATTTTCCACCACACTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAACCTGTTCAAAATCCACCGTGCAGTCAGAACCGTCAATCACCGTAccc                                                                                                                                                                 <  1:252205/203‑1 (MQ=255)                                                                                                                                    cTAAATTCAAATTTGGCAGTTAATTTGAAGCCATTTTCCACCACACTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAACCTGTTCAAAATCCACCGTGCAGTCAGAACCGTCAATCACCGTACCCACCTCGACACAACAGCAAACTTTTGCTTCTTCCGCCTGACATTTCAATGTGCTCataat                                                                                                      <  1:585318/251‑1 (MQ=255)                                                                                                                                    cTAAATTCAAATTTGGCAGTTAATTTGAAGCCATTTTCCACCACACTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAACCTGTTCAAAATCCACCGTGCAGTCAGAACCGTCAATCACCGTACCCACCTCGACACAACAGCAAACTTTTGCTTCTTCCGCCTGACATTTCAATGTGCTCataat                                                                                                      <  1:517508/251‑1 (MQ=255)                                                                                                                                    cTAAATTCAAATTTGGCAGTTAATTTGAAGCCATTTTCCACCACACTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAACCTGTTCAAAATCCACCGTGCAGTCAGAACCGTCAATCACCGTACCCACCTCGACACAACAGCAAACTTTTGCTTCTTCCGCCTGACATTTCAATGTGCTCataat                                                                                                      <  1:477344/251‑1 (MQ=255)                                                                                                                                    cTAAATTCAAATTTGGCAGTTAATTTGAAGCCATTTTCCACCACACTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAACCTGTTCAAAATCCACCGTGCAGTCAGAACCGTCAATCACCGTACCCACCTCGACACAACAGCAAACTTTTGCTTCTTCCGCCTGACATTTCAATGTGCTCata                                                                                                        <  1:174744/249‑1 (MQ=255)                                                                                                                                        aTTCAAATTTGGCAGTTAATTTGAAGCCATTTTCCACCACACTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAAcc                                                                                                                                                                                                              <  1:61589/143‑1 (MQ=255)                                                                                                                                                    cAGTTAATTTGAAGCCATTTTCCACCACACTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAACCTGTTCAAAATCCACCGTGCAGTCAGAACCGTCAATCACCGTACCCACCTCGACacaac                                                                                                                                                    >  1:218856/1‑189 (MQ=255)                                                                                                                                                           tttGAAGCCATTTTCCACCACACTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAACCTGTTCAAAATCCACCGTGCAGTCAGAACCGTCAATCACCGTACCCACCTCGACACAACAGCAAACTTTTGCTTCTTCCGCCTGACATTTCAATGTGCTCATAATAtc                                                                                                   >  1:115365/1‑231 (MQ=255)                                                                                                                                                                 gCCATTTTCCACCACACTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAACCTGTTCAAAATCCACCGTGCAGTCAGAACCGTCAATCACCGTACCCACCTCGACACAACAGCAAACTTTTGCTTCTTCCGCCTGACATTTCAATGTGCTCATAATAtc                                                                                                   <  1:27660/225‑1 (MQ=255)                                                                                                                                                                    aTTTTCCACCACACTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAACCTGTTCAAAATCCACCGTGCAGTCAGAACCGTCAATCACCGTACCCACCTCGACACAACAGCAAACTTTTGCTTCTTCCGCCTGACATTTCAATGTGCTCATAATAtctc                                                                                                 <  1:631974/224‑1 (MQ=255)                                                                                                                                                                       ttCCACCACACTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAACCTGTTCAAAATCCACCGTGCAGTCAGAACCGTCAATCACCGTACCCACCTCGACACAACAGCAAACTTTTGCTTCTTCCGCCTGACATTTCAAt                                                                                                                 <  1:639707/205‑1 (MQ=255)                                                                                                                                                                            ccacACTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAACCTGTTCAAAATCCACCGTGCAGTCAGAACCGTCAATCACCGTAC‑‑‑CCTCGACACAACAGCAAACTTTTGCTTCTTCCGCCTGACATTTCAATGTGCTCATAATATCTCCTTGTAAAAAGAATTGAAATTTTCAACTATTTTAGCat                                                           <  1:818274/251‑1 (MQ=255)                                                                                                                                                                              acacTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAACCTGTTCAAAATCCACCGTGCAGTCAGAAc                                                                                                                                                                                  >  1:123351/1‑133 (MQ=255)                                                                                                                                                                              acacTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAACCTGTTCAAAATCCACCGTGCAGTCAGAACCGTCAATCACCGTACCCACCTCGACACAACAGCAAACTTTTGCTTCTTCCGCCTGACATTTCAATGTGCTCATAATATCTCCTTGTAAAAAGAATTGAAATTTTCAACTATTTTAGCa                                                            <  1:782763/251‑1 (MQ=255)                                                                                                                                                                               cacTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAACCTGTTCAAAATCCACCGTGCAGTCAGAACCGTCAATCACCGTACCCACCTCGacac                                                                                                                                                       >  1:680291/1‑159 (MQ=255)                                                                                                                                                                               cacTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAACCTGTTCAAAATCCACCGTGCAGTCAGAACCGTCAATCACCGTACCCACCTCGACacaac                                                                                                                                                    >  1:243260/1‑162 (MQ=255)                                                                                                                                                                               cacTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAACCTGTTCAAAATCCACCGTGCAGTCAGAACCGTCAATCACCGTACCCACCTCGACACAACAGCAAACTTTTGCTTCTTCCGCCTGACATTTCAATGTGCTCATAATATCTCCTTGTAAAAAGAATTGAAATTTTCAACTATTTTAGCat                                                           <  1:305261/251‑1 (MQ=255)                                                                                                                                                                               cacTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAACCTGTTCAAAATCCACCGTGCAGTCAGAACCGTCAATCACCGTACCCACCTCGACACAACAGCAAACTTTTGCTTCTTCCGCCTGACATTTCAATGTGCTCATAATATCTCCTTGTAAAAAGAATTGAAATTTTCAACTATTTTAGCa                                                            >  1:773266/1‑250 (MQ=255)                                                                                                                                                                                     tttCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAACCTGTTCAAAATCCACCGTGCAGTCAGAACCGTCAATCACCGTACCCACCTCGACACAACAGCAAACTTTTGCTTCTTCCGCCTGACATTTCaa                                                                                                                  >  1:275762/1‑190 (MQ=255)                                                                                                                                                                                         actactGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAACCTGTTCAAAATCCACCGTGCAGTCAGAACCGTCAATCACCGTACCCACCTCGACACAACAGCAAACTTTTGCTTCTTCCGCCTGACATTTCAATGTGCTCATAATATCTCCTTGTAAAAAGAATTGAAATTTTCAACTATTTTAGCATTATTCAAAtt                                                 >  1:101732/1‑251 (MQ=255)                                                                                                                                                                                          ctactGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAACCTGTTCAAAATCCACCGTGCAGTCAGAACCGTCAATCACCGTACCCACCTCGACACAACAGCAAACTTTTGCTTCTTCCGCCTGACATTTCAATGTGCTCATAATATCTCCTTGTAAAAAGAATTGAAATTTTCAACTATTTTAGCATTATTCAAAttt                                                <  1:155180/251‑1 (MQ=255)                                                                                                                                                                                                aTTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAACCTGTTCAAAATCCACCGTGCAGTCAGAACCGTCAATCACCGTACCCACCTCGACACAACAGCAAACTTTTGCTTCTTCCGCCTGACATTTCAATGTGCTCATAATAtctc                                                                                                 <  1:683413/196‑1 (MQ=255)                                                                                                                                                                                                          gATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAACCTGTTCAAAATCCACCGTGCAGTCAGAACCGTCAATCACCGTACCCACCTCGACACAACAGCAAACTTTTGCTTCTTCCGCCTGACATTTCAATGTGCTCATAATATCTCCTTGTAAAAAGAATTGaaa                                                                              >  1:707246/1‑205 (MQ=255)                                                                                                                                                                                                              aCTTTCCGCCGCTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAACCTGTTCAAAATCCACCGTGCAGTCAGAACCGTCAATCACCGTACCCACCTCGACACAACAGCAAACTTTTGCTTCTTCCGCCTGACATTTCAATGTGCTc                                                                                                           >  1:186273/1‑172 (MQ=255)                                                                                                                                                                                                                        gcTTTGGCTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAACCTGTTCAAAATCCACCGTGCAGTCAGAACCGTCAATCACCGTACCCACCTCGACACAACAGCAAACTTTTGCTTCTTCCGCCTGACATTTCAATGTGCTCATAATAtctc                                                                                                 >  1:872566/1‑172 (MQ=255)                                                                                                                                                                                                                               cTTTTTCGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAACCTGTTCAAAATCCACCGTGCAGTCAGAACCGTCAATCACCGTACCCACCTCGACACAACAGCAAACTTTTGCTTCTTCCGCCTGACATTTCAATGTGCTCATAATATCTCCTTGTAAAAAGAATTGAAATTTTCAACTATTTTAGCATTATTCAAATTTAATACAAAATCCGACCGCACTTTTCTTTGTCCACTgg           <  1:459192/251‑1 (MQ=255)                                                                                                                                                                                                                                     cGGTTAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAACCTGTTCAAAATCCACCGTGCAGTCAGAACCGTCAATCACCGTACCCACCTCGACACAACAGCAAACTTTTGCTTCTTCCGCCTGACATTTCAATGTGCTCATAATATCTCCTTGTAAAAAGAATTGAAATTTTCAACTATTTTAGCATTATTCAAATTTAATACAAAATCCGACCGCACTTTTCTTTGTCCACTGGTCTGaa     >  1:72143/1‑251 (MQ=255)                                                                                                                                                                                                                                        ttAAACAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAACCTGTTCAAAATCCACCGTGCAGTCAGAACCGTCAATCACCGTACCCACCTCGACACAACAGCAAACTTTTGCTTCTTCCGCCTGACATTTCAATGTGCTCATAATATCTCCTTGTAAAAAGAATTGAAATTTTCAACTATTTTAGCATTATTCAAATTTAATACAAAATCCGACCGCACTTTTCTTTGTCCACTGGTCTGAacac  <  1:117938/251‑1 (MQ=255)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                      GTTAATTTCATCGTAAATAGAATACATCGAGTTATAATAAAAAAGGCTTAAGTTTTCGACTTAAGCCTTTTTTATTTATATCAATCAATTAACGGGTAGAAAGTTGGAAAATCATGGATTCCGCCTGGCAGCTAAATTCAAATTTGGCAGTTAATTTGAAGCCATTTTCCACCACACTTATTTCACTACTGATTTTGCATGGATCACTTTCCGCCGCTTTGGCTTTTTCGGTTAAATAGCTTAATGCTTCTTTTGCTGCTTCTTCCGTTGCATAAACCTGTTCAAAATCCACCGTGCAGTCAGAACCGTCAATCACCGTACCCACCTCGACACAACAGCAAACTTTTGCTTCTTCCGCCTGACATTTCAATGTGCTCATAATATCTCCTTGTAAAAAGAATTGAAATTTTCAACTATTTTAGCATTATTCAAATTTAATACAAAATCCGACCGCACTTTTCTTTGTCCACTGGTCTGAACAC  >  1/1231214‑1231695

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑