Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,230,286	G→A	A156V (GCG→GTG)	unknown ←	glycosyl transferase, group 2 family

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,230,286	0	G	A	100.0%	32.4 / NA	14	A156V (GCG→GTG)	unknown	glycosyl transferase, group 2 family
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (10/4);  total (10/4)

CGAGAAAAAACTTCCATCTTATATTATAAGGATCCTCTATTTGATTTATCTCTAATAGCCTTATATTTCTATCAATTTTTATAGGTATATTAACTATGTTATTTTTAGAATATGGGTATTCATGCGCTCTTCCATTGTATTTAATGAGTCCATTATTTTTGAATATTCTCCCAACACCATAATTTTTATTATTTTTAGAATCCAAAAACCTAGGGCAAAAAACTGTTGCGTCGTTGCATCTACCATACATAATAAGAAAGTCTCGAAGGTCTCTGTACGCTCTGCCATCAACCCACTCATCAGCATCTATAAAAAATACCCACGAATAGCCAGAACATTCCAGAGCAAAATTTCTTAATTCAGAAAAAT  >  1/1230161‑1230529                                                                                                                              |                                                                                                                                                                                                                                                    cGAGAAAAAACTTCCATCTTATATTATAAGGATCCTCTATTTGATTTATCTCTAATAGCCTTATATTTCTATCAATTTTTATAGGTATATTAACTATGTTATTTTTAGAATATGGGTATTCATGCACTCTTCCATTGTATTTAATGAGTCCATTATTTTTGAATATTCTCCCAACACCATAATTTTTATTATTTTTAGAATCCAAAAACCTAGGGCAAAAAACTGTTGCGTCGTTGCATCTACcatacat                                                                                                                         <  1:867352/250‑1 (MQ=255)                           taAGGATCCTCTATTTGATTTATCTCTAATAGCCTTATATTTCTATCAATTTTTATAGGTATATTAACTATGTTATTTTTAGAATATGGGTATTCATGCACTCTTCCAt                                                                                                                                                                                                                                            <  1:117674/109‑1 (MQ=255)                                                aTCTCTAATAGCCTTATATTTCTATCAATTTTTATAGGTATATTAACTATGTTATTTTTAGAATATGGGTATTCATGCACTCTTCCATTGTATTTAATGAGTCCATTATTTTTGAATATTCTCCCAACACCATAATTTTTATTATTTTTAGAATCCAAAAACCTAGGGCAAAAAACTGTTGCGTCGTTGCATCTACCATACATAATAAGAAAGTCTCGAAGGTCTCTGTACGCTCTGCCATCAACCCACt                                                                          >  1:676206/1‑250 (MQ=255)                                                    ctAATAGCCTTATATTTCTATCAATTTTTATAGGTATATTAACTATGTTATTTTTAGAATATGGGTATTCATGCACTCTTCCATTGTATTTAATGAGTCCATTATTTTTGAATATTCTCCCAACACCATAATTTTTATTATTTTTAGAATCCAAAAACCTAGGGCAAAAAACTGTTGCGTCGTTGCATCTACCATACATAATAAGAAAGTCTCGAAGGTCTCTGTACGc                                                                                           >  1:49440/1‑229 (MQ=255)                                                                                    ggTATATTAACTATGTTATTTTTAGAATATGGGTATTCATGCACTCTTCCATTGTATTTAATGAGTCCATTATTTTTGAATATTCTCCCAACACCATAATTTTTATTATTTTTAGAATCCAAAAACCTAGGGCAAAAAACTGTTGCGTCGTTGCATCTACCATACATAATAAGAAAGTCTCGAAGGTCTCTGTACGCTCTGCCATCAAc                                                                               >  1:231494/1‑209 (MQ=255)                                                                                     gTATATTAACTATGTTATTTTTAGAATATGGGTATTCATGCACTCTTCCATTGTATTTAATGAg                                                                                                                                                                                                                               <  1:255800/64‑1 (MQ=255)                                                                                     gTATATTAACTATGTTATTTTTAGAATATGGGTATTCATGCACTCTTCCATTGTATTTAATGAGTCCATTATTTTTGAATATTCTCCCAacac                                                                                                                                                                                                  <  1:450967/93‑1 (MQ=255)                                                                                     gTATATTAACTATGTTATTTTTAGAATATGGGTATTCATGCACTCTTCCATTGTATTTAATGAGTCCATTATTTTTGAATATTCTCCCAACACCATAATTTTTATTATTTTTAGAATCCAAAAACCTAGGGCAAAAAACTGTTGCGTCGTTGCATCTACcatac                                                                                                                           >  1:827990/1‑164 (MQ=255)                                                                                     gTATATTAACTATGTTATTTTTAGAATATGGGTATTCATGCACTCTTCCATTGTATTTAATGAGTCCATTATTTTTGAATATTCTCCCAACACCATAATTTTTATTATTTTTAGAATCCAAAAACCTAGGGCAAAAAACTGTTGCGTCGTTGCATCTACCATACATAATAAGAAAGTCTCGAAGGTCTCTGTACGCTCTGCCATCAACCCACTCATCAGCATCTATaaaa                                                         >  1:364142/1‑230 (MQ=255)                                                                                     gTATATTAACTATGTTATTTTTAGAATATGGGTATTCATGCACTCTTCCATTGTATTTAATGAGTCCATTATTTTTGAATATTCTCCCAACACCATAATTTTTATTATTTTTAGAATCCAAAAACCTAGGGCAAAAAACTGTTGCGTCGTTGCATCTACCATACATAATAAGAAAGTCTCGAAGGTCTCTGTACGCTCTGCCATCAACCCACTCATCAGCATCTATAAAAAATACCCACGAATAGcc                                        >  1:763280/1‑247 (MQ=255)                                                                                                aTGTTATTTTTAGAATATGGGTATTCATGCACTCTTCCATTGTATTTAATGAGTCCATTATTTTTGAATATTCTCCCAACACCATAATTTTTATTATTTTTAGAATCCAAAAACCTAGGGCAAAAAACTGTTGCGTCGTTGCATCTACCATACATAATAAGAAAGTCTCGAAGGTCTCTGTACGCTCTGCCATCAACCCACTCATCAGCATCTATAAAAAATACCCACGAATAGCCAGAAc                                   >  1:584146/1‑241 (MQ=255)                                                                                                            gAATATGGGTATTCATGCACTCTTCCATTGTATTTAATGAGTCCATTATTTTTGAATATTCTCCCAACACCATAATTTTTATTATTTTTAGAATCCAAAAACCTAGGGCAAAAAACTGTTGCGTCGTTGCATCTACCATACACAATAAGAAAGTCTCGAAGGTCTCTGTCCGCTCTGCCATCAACCCACTCATCAGCATCTATAAAAAATACCCACGAATAGCCAGAACATTCCAGAGCAAAATTTCTTaa             >  1:446835/1‑251 (MQ=255)                                                                                                                       ttCATGCACTCTTCCATTGTATTTAATGAGTCCATTATTTTTGAATATTCTCCCAACACCATAATTTTTATTATTTTTAGAATCCAAAAACCTAGGGCAAAAAACTGTTGCGTCGTTGCATCTACCATACATAATAAGAAAGTCTCGAAGGTCTCTGTACGCTCTGCCATCAACCCACTCATCAGCATCTATAAAAAATACCCACGAATAGCCAGAACATTCCAGAGCAAAATTTCTTAATTCAGAAGAAt  >  1:430814/1‑251 (MQ=255)                                                                                                                       ttCATGCACTCTTCCATTGTATTTAATGAGTCCATTATTTTTGAATATTCTCCCAACACCATAATTTTTATTATTTTTAGAATCCAAAAACCTAGGGCAAAAAACTGTTGCGTCGTTGCATCTACCATACATAATAAGAAAGTCTCGAAGGTCTCTGTACGCTCTGCCATCAACCCACTCATCAGCATCTATAAAAAATACCCACGAATAGCCAGAACATTCCAGAGCAAAATTTCTTAATTCAGAAAAAt  >  1:314288/1‑251 (MQ=255)                                                                                                                              |                                                                                                                                                                                                                                                    CGAGAAAAAACTTCCATCTTATATTATAAGGATCCTCTATTTGATTTATCTCTAATAGCCTTATATTTCTATCAATTTTTATAGGTATATTAACTATGTTATTTTTAGAATATGGGTATTCATGCGCTCTTCCATTGTATTTAATGAGTCCATTATTTTTGAATATTCTCCCAACACCATAATTTTTATTATTTTTAGAATCCAAAAACCTAGGGCAAAAAACTGTTGCGTCGTTGCATCTACCATACATAATAAGAAAGTCTCGAAGGTCTCTGTACGCTCTGCCATCAACCCACTCATCAGCATCTATAAAAAATACCCACGAATAGCCAGAACATTCCAGAGCAAAATTTCTTAATTCAGAAAAAT  >  1/1230161‑1230529

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑