Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,224,664	T→C	G20G (GGT→GGC)	unknown →	Phospholipid ABC transporter substrate‑binding protein MlaD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,224,664	0	T	C	100.0%	220.0 / NA	81	G20G (GGT→GGC)	unknown	Phospholipid ABC transporter substrate‑binding protein MlaD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (44/37);  total (44/37)

GTTCTTCGCCATTGCGGTGGTTTGGATTGCATTGTTTAACGGCTACGACTGTATTCCGACTTCGGAAGGCATCAGCAAAGCCACCACCAGAACCGTTGTACACGCGTCATTAGTAGTATTAGGTTTAGACTTCGTACTGACCGCCATCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGTTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACGCGCCCCGCTTAAAGTGGGCGGCGTTGTGATTGGGCGGGTATCCGAAATCACATTGGATCCGCAAACGTATTTACCGAAAGTCACCATCGCCATTAATCAGGAATACAATGAAATTCCGGAAACCAGTTCGTTATC  >  1/1224417‑1224906                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   gTTCTTCGCCATTGCGGTGGTTTGGATTGCATTGTTTAACGGCTACGACTGTATTCCGACTTCGGAAGGCATCAGCAAAGCCACCACCAGAACCGTTGTACACGCGTCATTAGTAGTATTAGGTTTAGACTTCGTACTGACCGCCATCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCg                                                                                                                                                                                                                                                 >  1:505281/1‑251 (MQ=255)      tcGCCATTGCGGTGGTTTGGATTGCATTGTTTAACGGCTACGACTGTATTCCGACTTCGGAAGGCATCAGCAAAGCCACCACCAGAACCGTTGTACACGCGTCATTAGTAGTATTAGGTTTAGACTTCGTACTGACCGCCATCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTAt                                                                                                                                                                                                                                            >  1:347744/1‑251 (MQ=255)                             gCATTGTTTAACGGCTACGACTGTATTCCGACTTCGGAAGGCATCAGCAAAGCCACCACCAGAACCGTTGTACACGCGTCATTAGTAGTATTAGGTTTAGACTTCGTACTGACCGCCATCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGa                                                                                                                                                                                                                     >  1:14906/1‑251 (MQ=255)                               aTTGTTTAACGGCTACGACTGTATTCCGACTTCGGAAGGCATCAGCAAAGCCACCACCAGAACCGTTGTACACGCGTCATTAGTAGTATTAGGTTTAGACTTCGTACTGACCGCCATCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAAc                                                                                                                                                                                                                   <  1:202039/251‑1 (MQ=255)                                  gTTTAACGGCTACGACTGTATTCCGACTTCGGAAGGCATCAGCAAAGCCACCACCAGAACCGTTGTACACGCGTCATTAGTAGTATTAGGTTTAGACTTCGTACTGACCGCCATCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTAtcttc                                                                                                                                                                                                                                        >  1:792704/1‑227 (MQ=255)                                  gTTTAACGGCTACGACTGTATTCCGACTTCGGAAGGCATCAGCAAAGCCACCACCAGAACCGTTGTACACGCGTCATTAGTAGTATTAGGTTTAGACTTCGTACTGACCGCCATCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTg                                                                                                                                                                                                                >  1:157391/1‑251 (MQ=255)                                       aCGGCTACGACTGTATTCCGACTTCGGAAGGCATCAGCAAAGCCACCACCAGAACCGTTGTACACGCGTCATTAGTAGTATTAGGTTTAGACTTCGTACTGACCGCCATCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAgg                                                                                                                                                                                                           <  1:180037/251‑1 (MQ=255)                                         ggCTACGACTGTATTCCGACTTCGGAAGGCATCAGCAAAGCCACCACCAGAACCGTTGTACACGCGTCATTAGTAGTATTAGGTTTAGACTTCGTACTGACCGCCATCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAg                                                                                                                                                                                                                           <  1:191277/233‑1 (MQ=255)                                         ggCTACGACTGTATTCCGACTTCGGAAGGCATCAGCAAAGCCACCACCAGAACCGTTGTACACGCGTCATTAGTAGTATTAGGTTTAGACTTCGTACTGACCGCCATCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGtt                                                                                                                                                                                                         >  1:837441/1‑251 (MQ=255)                                         ggCTACGACTGTATTCCGACTTCGGAAGGCATCAGCAAAGCCACCACCAGAACCGTTGTACACGCGTCATTAGTAGTATTAGGTTTAGACTTCGTACTGACCGCCATCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGtt                                                                                                                                                                                                         >  1:490286/1‑251 (MQ=255)                                                aCTGTATTCCGACTTCGGAAGGCATCAGCAAAGCCACCACCAGAACCGTTGTACACGCGTCATTAGTAGTATTAGGTTTAGACTTCGTACTGACCGCCATCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGc                                                                                                                                                                                                                                  >  1:880531/1‑219 (MQ=255)                                                         cGACTTCGGAAGGCATCAGCAAAGCCACCACCAGAACCGTTGTACACGCGTCATTAGTAGTATTAGGTTTAGACTTCGTACTGACCGCCATCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATc                                                                                                                                                                                         <  1:228645/251‑1 (MQ=255)                                                                     gCATCAGCAAAGCCACCACCAGAACCGTTGTACACGCGTCATTAGTAGTATTAGGTTTAGACTTCGTACTGACCGCCATCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTa                                                                                                                                                                             >  1:890517/1‑251 (MQ=255)                                                                        tCAGCAAAGCCACCACCAGAACCGTTGTACACGCGTCATTAGTAGTATTAGGTTTAGACTTCGTACTGACCGCCATCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTAtac                                                                                                                                                                          <  1:502734/251‑3 (MQ=255)                                                                        tCAGCAAAGCCACCACCAGAACCGTTGTACACGCGTCATTAGTAGTATTAGGTTTAGACTTCGTACTGACCGCCATCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGc                                                                                                                                                                          <  1:355124/251‑1 (MQ=255)                                                                        tCAGCAAAGCCACCACCAGAACCGTTGTACACGCGTCATTAGTAGTATTAGGTTTAGACTTCGTACTGACCGCCATCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGc                                                                                                                                                                          <  1:502745/251‑1 (MQ=255)                                                                         cAGCAAAGCCACCACCAGAACCGTTGTACACGCGTCCTTAGTAGTATTAGGTTTAGACTTCGTACTGACCGCCATCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTCGGCTTAAAAGTGGCGAACGTGCAAGGttttcg                                                                                                                                                                                                     >  1:454941/1‑220 (MQ=255)                                                                                gCCACCACCAGAACCGTTGTACACGCGTCATTAGTAGTATTAGGTTTAGACTTCGTACTGACCGCCATCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTTTCTTCTTAGGCTTAAAAGTGGCGAACGTGCCAGGGTTCAGTGAAGCCAAATCTTATCACGTTTATGCAACTTTCg                                                                                                                                                                  >  1:282962/1‑251 (MQ=255)                                                                                             ccGTTGTACACGCGTCATTAGTAGTATTAGGTTTAGACTTCGTACTGACCGCCTTCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTgg                                                                                                                                                     >  1:261066/1‑251 (MQ=255)                                                                                               gTTGTACACGCGTCATTAGTAGTATTAGGTTTAGACTTCGTACTGACCGCCATCATGTTCGGTGCGAATTAATCTCTGATTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGa                                                                                                                                                    >  1:510786/1‑250 (MQ=255)                                                                                                  gTACACGCGTCATTAGTAGTATTAGGTTTAGACTTCGTACTGACCGCCATCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTaa                                                                                                                                                >  1:587627/1‑251 (MQ=255)                                                                                                       cgcgTCATTAGTAGTATTAGGTTTAGACTTCGTACTGACCGCCATCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAGGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGttt                                                                                                                                                                              >  1:442755/1‑216 (MQ=255)                                                                                                       cgcgTCATTAGTAGTATTAGGTTTAGACTTCGTACTGACCGCCATCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACg                                                                                                                                                                                 >  1:591342/1‑213 (MQ=255)                                                                                                                  tagtaTTAGGTTTAGACTTCGTACTGACCGCCATCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACGCGCCCCGCt                                                                                                                                <  1:354165/251‑1 (MQ=255)                                                                                                                   agtaTTAGGTTTAGACTTCGTACTGACCGCCATCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAg                                                                                                                                                                                                     <  1:271645/181‑1 (MQ=255)                                                                                                                    gtaTTAGGTTTAGACTTCGTACTGACCGCCATCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGat                                                                                                                                                                <  1:198721/217‑1 (MQ=255)                                                                                                                       ttAGGTTTAGACTTCGTACTGACCGCCATCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACGCGCCCCGCTTAAAg                                                                                                                           <  1:267200/251‑1 (MQ=255)                                                                                                                       ttAGGTTTAGACTTCGTACTGACCGCCATCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACGCGCCCCGCTTAAAg                                                                                                                           <  1:424816/251‑1 (MQ=255)                                                                                                                       ttAGGTTTAGACTTCGTACTGACCGCCATCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACGCGCCCCGCTTAAAg                                                                                                                           <  1:136648/251‑1 (MQ=255)                                                                                                                         aacccgacgtagcggatccaCCGCCATCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACGCGCCCCGCTTAAAGTg                                                                                                                         <  1:163595/232‑1 (MQ=255)                                                                                                                          ggTTTAGACTTCGTACTGACCGCCATCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGc                                                                                                                                                                          >  1:558596/1‑201 (MQ=255)                                                                                                                          ggTTTAGACTTCGTACTGACCGCCATCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACGCGCCCCGCTTAAAGTgg                                                                                                                        >  1:91169/1‑251 (MQ=255)                                                                                                                          ggTTTAGACTTCGTACTGACCGCCATCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGGTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACGCGCCCCGCTTAAAGTgg                                                                                                                        >  1:189441/1‑251 (MQ=255)                                                                                                                            tttAGACTTCGTACTGACCGCCATCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACGCGCCCCGCTTAAAGTGggc                                                                                                                      <  1:15961/251‑1 (MQ=255)                                                                                                                                 aCTTCGTACTGACCGCCATCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACGCGCCCCGCTTAAAGTGGGCGGCGt                                                                                                                 >  1:748919/1‑251 (MQ=255)                                                                                                                                       tACTGACCGCCATCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACGCGCCCCGCTTAAAGTGGGCGGCGTTGTGa                                                                                                            <  1:12670/250‑1 (MQ=255)                                                                                                                                       tACTGACCGCCATCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACGCGCCCCGCTTAAAGTGGGCGGCGTTGTGa                                                                                                            <  1:618495/250‑1 (MQ=255)                                                                                                                                       tACTGACCGCCATCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACGCGCCCCGCTTAAAGTGGGCGGCGTTGTGAt                                                                                                           <  1:788526/251‑1 (MQ=255)                                                                                                                                       tACTGACCGCCATCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACGCGCCCCGCTTAAAGTGGGCGGCGTTGTGAt                                                                                                           <  1:307438/251‑1 (MQ=255)                                                                                                                                             ccGCCATCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACGCGcc                                                                                                                                     >  1:152599/1‑219 (MQ=255)                                                                                                                                             ccGCCATCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGGGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGata                                                                                                                                                               >  1:122608/1‑193 (MQ=255)                                                                                                                                                ccATCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGCTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACGCGCCCCGCCTAAAGTGGGCGGCGTTGTGATTGGGCgggg                                                                                                  >  1:831024/1‑250 (MQ=255)                                                                                                                                                  atcatGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGata                                                                                                                                                               >  1:261692/1‑188 (MQ=255)                                                                                                                                                  atcatGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACGCGCCCCGCTTAAAGTGGGCGGCGTTGTGATTGGGCGGGTAt                                                                                                >  1:607394/1‑251 (MQ=255)                                                                                                                                                  atcatGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACGCGCCCCGCTTAAAGTGGGCGGCGTTGTGATTGGGCGGGTAt                                                                                                >  1:288654/1‑251 (MQ=255)                                                                                                                                                      tGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTGTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACGCGCCCCGCTTAAAGTGGGCGGCGTTGTGATTGGGCGGGTATCCGa                                                                                            <  1:446809/251‑1 (MQ=255)                                                                                                                                                       gTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTAtcttc                                                                                                                                                                                                                                        <  1:503216/110‑1 (MQ=255)                                                                                                                                                            gTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACGCGCCCCGCTTAAAGt                                                                                                                          <  1:207577/215‑1 (MQ=255)                                                                                                                                                            gTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACGCGCCCCGCTTAAAGTGGGCGGCGTTGTGATTGGGCGGGTATCCGAAATca                                                                                       >  1:888700/1‑250 (MQ=255)                                                                                                                                                            gTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGGGGACTAAAAGTACGCGCCCCGCTTAAAGTGGGCGGCGTTGTGATTGGGCGGGTATCCGAAATca                                                                                       >  1:897190/1‑250 (MQ=255)                                                                                                                                                                 aaTTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACGCGCCCCGCTTAAAGTGGGCGGCGTTGTGATTGGGCGGGTATCCGGAATCACATTgg                                                                                 >  1:736490/1‑251 (MQ=255)                                                                                                                                                                     aaTCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATTTTATCACGTTTATGCAACTTTCGat                                                                                                                                                                >  1:471836/1‑168 (MQ=255)                                                                                                                                                                      aTCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACGCGCCCCGCTTAAAGTGGGCGGCGTTGTGATTGGGCGGGTATCCGAAATCACATTGGATCCg                                                                            <  1:163623/251‑1 (MQ=255)                                                                                                                                                                       tctcTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGataa                                                                                                                                                              <  1:333067/168‑1 (MQ=255)                                                                                                                                                                            gTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATc                                                                                                                                                                                         <  1:213085/136‑1 (MQ=255)                                                                                                                                                                            gTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACgcgc                                                                                                                                      >  1:684854/1‑187 (MQ=255)                                                                                                                                                                            gTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGGGGACTAAAAGTACGCGCCCCGCTTAAAGTGGGCGGCGGTGTGATTGGGCGGGTATCCGAAATCACATTGGATCCGCaaaag                                                                      >  1:881587/1‑249 (MQ=255)                                                                                                                                                                                   aGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGAACGCGCCCCGCTTAAAGTGGGCGGCGTTGTGATTTGGCGGGTATCCGAAATCACATTGGATCCGCAAACGTATTTa                                                                >  1:418734/1‑250 (MQ=255)                                                                                                                                                                                        aCGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACGCGCCCCGCTTAAAGTGGGCGGCGTTGTGATTGGGCGGGTATCCGAAATCACATTGGATCCGCAAACGTATTTACCGaaa                                                          >  1:475316/1‑251 (MQ=255)                                                                                                                                                                                         cGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAatat                                                                                                                                                           >  1:281303/1‑153 (MQ=255)                                                                                                                                                                                         cGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACGCGCCCCGCTTAAAGTGGGCGGCGTTGTGATTGGGCGGGTATCCGAAATCACATTGGATCCGCAAACGTATTTACCGAAAg                                                         <  1:415547/251‑1 (MQ=255)                                                                                                                                                                                                         aTTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTAcgc                                                                                                                                        >  1:587751/1‑156 (MQ=255)                                                                                                                                                                                                         aTTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACGCGCCCCGCTTAAAGTGGGCGGCGTTGTGATTGGGCGGGTATCCGAAATCACAt                                                                                    <  1:692272/208‑1 (MQ=255)                                                                                                                                                                                                         aTTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACGCGCCCCGCTTAAAGTGGGCGGCGTTGTGATTGGGCGGGTATCCGAAATCACAt                                                                                    <  1:152913/208‑1 (MQ=255)                                                                                                                                                                                                         aTTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACGCGCCCCGCTTAAAGTGGGCGGCGTTGTGATTGGGCGGGTATCCGAAATCACATTGGATCCGCAAACGTATTTACCGAAAGGCACCATCGCCATTaa                                         >  1:145566/1‑251 (MQ=255)                                                                                                                                                                                                               tatGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAg                                                                                                                                             <  1:489473/145‑1 (MQ=255)                                                                                                                                                                                                               tatGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACGCGCCCCGCTTAAAGTGGGCGGCGTTGTGATTGGGCGGGTATCCGAAATCACATTGGATCCGCAAACGTATTTACCGAAAg                                                         <  1:380935/229‑1 (MQ=255)                                                                                                                                                                                                               tatGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACGCGCCCCGCTTAAAGTGGGCGGCGTTGTGATTGGGCGGGTATCCGAAATCACATTGGATCCGCAAACGTATTTACCGAAAGTCACCATCGCCATTAATc                                       >  1:624190/1‑247 (MQ=255)                                                                                                                                                                                                                  gAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGc                                                                                                                                                                          <  1:883154/113‑1 (MQ=255)                                                                                                                                                                                                                   aaTTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACGCGCCCCGCTTAAAGTGGGCGGCGTTGTGATTGGGCGGGTATCCGAAATCACATTGGATCCGCAAACGTATTTACCGAAAGTCACCATCGCCATTAATCAGGAATAc                               <  1:544233/251‑1 (MQ=255)                                                                                                                                                                                                                      tttGGGTCGGTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACGCGCCCCGCTTAAAGTGGGCGGCGTTGTGATTGGGCGGGTATCCGAAATCACATTGGATCCGCAAACGTATTTACCGAAAGTCACCATCGCCATTAATCAGGAATACAAt                            <  1:479845/251‑1 (MQ=255)                                                                                                                                                                                                                               gTTTATTTTTACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACGCGCCCCGCTTAAAGTGGGCGGCGTTGTGATTGGGCGGGTATCCGAAATCACATTGGATCCGCAAACGTATTTACCGAAAGTCACCATCGCCATTAATCAGGAATACAATGAAATTCCg                   >  1:296566/1‑251 (MQ=255)                                                                                                                                                                                                                                    tttttACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACGCGCCCCGCTTAAAGTGGGCGGCGTTGTGATTGGGCGGGTATCCGAAATCACATTGGATCCGCAAACGTATTTACCGAAAGTCAcc                                                    <  1:357191/213‑1 (MQ=255)                                                                                                                                                                                                                                    tttttACTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACGCGCCCCGCTTAAAGTGGGCGGCGTTGTGATTGGGCGGGTATCCGAAATCACATTGGATCCGCAAACGTATTTACCGAAAGTCACCATCGCCATTAATCAGGAATACAATGAAATTCCGGaaa               >  1:589851/1‑250 (MQ=255)                                                                                                                                                                                                                                          cTCCTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACGCGCCCCGCTTAAAGTGGGCGGCGTTGTGATTGGGCGGGTATCCGAAATCACATTGGATCCGCAAACGTATTTAc                                                               <  1:563280/196‑1 (MQ=255)                                                                                                                                                                                                                                            ccTTGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACGCGCCCCGCTTAAAGTGGGCGGCGTTGTGATTGGGCGGGTATCCGAAATCACATTGGATCCGCAAACGTATTTACCGAAAGTCACCATCGCCATTAATCAGGAATACAATGAAATTCCGGAAACCAGTTCGt      >  1:83638/1‑251 (MQ=255)                                                                                                                                                                                                                                               tGGTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACGCGCCCCGCTTAAAGTGGGCGGCGTTGTGATTGGGCGGGTATCCGAAATCACATTGGATCCGCAAACGTATTTACCGAAAGTCACCATCGCCATTAATCAGGAATACAATGAAATTCCGGAAACCAGTTCGTTAt   <  1:211871/251‑1 (MQ=255)                                                                                                                                                                                                                                                 gTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAg                                                                                                                                             <  1:230297/111‑1 (MQ=255)                                                                                                                                                                                                                                                 gTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACGCGCCCCGCTTAAAGTGGGCGGCGTTGTGATTGGGCGGGTATCCGAAATCACATTGGATc                                                                              >  1:3297/1‑174 (MQ=255)                                                                                                                                                                                                                                                 gTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACGCGCCCCGCTTAAAGTGGGCGGCGTTGTGATTGGGCGGGTATCCGAAATCACATTGGATCCGCAAACGTATTTACCGAAAGTCACCAt                                                  >  1:218659/1‑202 (MQ=255)                                                                                                                                                                                                                                                 gTATCGGCTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACGCGCCCCGCTTAAAGTGGGCGGCGTTGTGATTGGGCGGGGATCCGAAATCACATTGGATCCGCAAACGTATTTACCGAAAGTCACCATCGCCCTTAATCAGGAATACAATGAAATTCCGGAAACCAGTTCGTTATc  >  1:220116/1‑250 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                                                                   GTTCTTCGCCATTGCGGTGGTTTGGATTGCATTGTTTAACGGCTACGACTGTATTCCGACTTCGGAAGGCATCAGCAAAGCCACCACCAGAACCGTTGTACACGCGTCATTAGTAGTATTAGGTTTAGACTTCGTACTGACCGCCATCATGTTCGGTGCGAATTAATCTCTGTTTGTAAGGAAACGTTATGCGACAAACAATTAAATATGAATTTTGGGTCGGTTTATTTTTACTCCTTGGTATCGGTTCGCTTATCTTCTTAGGCTTAAAAGTGGCGAACGTGCAAGGTTTCAGTGAAGCAAAATCTTATCACGTTTATGCAACTTTCGATAATATCGGTGGACTAAAAGTACGCGCCCCGCTTAAAGTGGGCGGCGTTGTGATTGGGCGGGTATCCGAAATCACATTGGATCCGCAAACGTATTTACCGAAAGTCACCATCGCCATTAATCAGGAATACAATGAAATTCCGGAAACCAGTTCGTTATC  >  1/1224417‑1224906

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑