Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,218,531	A→G	E121G (GAA→GGA)	unknown →	Exodeoxyribonuclease VII large subunit (EC 3.1.11.6)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,218,531	0	A	G	100.0%	211.8 / NA	73	E121G (GAA→GGA)	unknown	Exodeoxyribonuclease VII large subunit (EC 3.1.11.6)
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/37);  total (36/37)

GCATTGGTATTTAAGTTTAAAAGACGAAAACGCCCAAGTGCGCTGCGCCATGTTCCGCATGAAAAATCTGCGCGTGGGTTTTCGTCCGTCTAATGGCATGCAGGTATTGGTGCGTGCTAGTGTCAGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGAATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTACAACGTCGTGATCCGAGTCTGAAAGTCATTATTTATCCCACAGCCGTGCAAGGTAAAGATGCGGCGGCTGAAATCGCACAAATGATTGAAATCGCCAACCGGCGACAAGAGGCGGATGTATTAATCGTGGGG  >  1/1218292‑1218769                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                               gCATTGGTATTTAAGTTTAAAAGACGAAAACGCCCAAGTGCGCTGCGCCATGTTCCGCATGAAAAATCTGCGCGTGGGTTTTCGTCCGTCTAATGGCATGCAGGTATTGGTGCGTGCTAGTGTCAGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCt                                                                                                                                                                                                                                      >  1:346542/1‑250 (MQ=255)       gTATTTAAGTTTAAAAGACGAAAACGCCCAAGTGCGCTGCGCCATGTTCCGCATGAAAAATCTGCGCGTGGGTTTTCGTCCGTCTAATGGCATGCAGGTATTGGTGCGTGCTAGTGTCAGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCTGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAAt                                                                                                                                                                                                                                >  1:252618/1‑250 (MQ=255)       gTATTTAAGTTTAAAAGACGAAAACGCCCAAGTGCGCTGCGCCATGTTCCGCATGAAAAATCTGCGCGTGGGTTTTCGTCCGTCTAATGGCATGCAGGTATTGGTGCGTGCTAGTGTCAGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAAt                                                                                                                                                                                                                                >  1:538617/1‑250 (MQ=255)                                 cccAAGTGCGCTGCGCCATGTTCCGCATGAAAAATCTGCGCGTGGGTTTTCGTCCGTCTAATGGCATGCAGGTATTGGTGCGTGCTAGTGTCAGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCA‑CTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGt                                                                                                                                                                                                     >  1:874450/1‑250 (MQ=255)                                   cAAGTGCGCTGCGCCATGTTCCGCATGAAAAATCTGCGCGTGGGTTTTCGTCCGTCTAATGGCATGCAGGTATTGGTGCGTGCTAGTGTCAGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCa                                                                                                                                                                                                   >  1:92395/1‑251 (MQ=255)                                             gcgcCATGTTCCGCATGAAAAATCTGCGCGTGGGTTTTCGTCCGTCTAATGGCATGCAGGTATTGGTGCGTGCTAGTGTCAGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCa                                                                                                                                                                                                                                    >  1:19530/1‑208 (MQ=255)                                             gcgcCATGTTCCGCATGAAAAATCTGCGCGTGGGTTTTCGTCCGTCTAATGGCATGCAGGTATTGGTGCGTGCTAGTGTCAGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAgg                                                                                                                                                                                          >  1:651649/1‑250 (MQ=255)                                             gcgcCATGTTCCGCATGAAAAATCTGCGCGTGGGTTTTCGTCCGTCTAATGGCATGCAGGTATTGGTGCGTGCTAGTGTCAGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAgg                                                                                                                                                                                          >  1:628276/1‑250 (MQ=255)                                                ccATGTTCCGCATGAAAAATCTGCGCGTGGGTTTTCGTCCGTCTAATGGCATGCAGGTATTGGTGCGTGCTAGTGTCAGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCatt                                                                                                                                                                                      <  1:374987/251‑1 (MQ=255)                                                  aTGTTCCGCATGAAAAATCTGCGCGTGGGTTTTCGTCCGTCTAATGGCATGCAGGTATTGGTGCGTGCTAGTGTCAGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCattat                                                                                                                                                                                    >  1:200736/1‑251 (MQ=255)                                                    gTTCCGCATGAAAAATCTGCGCGTGGGTTTTCGTCCGTCTAATGGCATGCAGGTATTGGTGCGTGCTAGTGTCAGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCAttatta                                                                                                                                                                                  >  1:102765/1‑251 (MQ=255)                                                     ttCCGCATGAAAAATCTGCGCGTGGGTTTTCGTCCGTCTAATGGCATGCAGGTATTGGTGCGTGCTAGTGTCAGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCATCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCAttatta                                                                                                                                                                                  <  1:569574/250‑1 (MQ=255)                                                     ttCCGCATGAAAAATCTGCGCGTGGGTTTTCGTCCGTCTAATGGCATGCAGGTATTGGTGCGTGCTAGTGTCAGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCAttatta                                                                                                                                                                                  <  1:108381/250‑1 (MQ=255)                                                     ttCCGCATGAAAAATCTGCGCGTGGGTTTTCGTCCGTCTAATGGCATGCAGGTATTGGTGCGTGCTAGTGTCAGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTAc                                                                                                                                                                                 <  1:22071/251‑1 (MQ=255)                                                              aaaaaTCTGCGCGTGGGTTTTCGTCCGTCTAATGGCATGCAGGTATTGGTGCGTGCTAGTGTCAGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAAc                                                                                                                                                                        <  1:872905/251‑1 (MQ=255)                                                                aaaTCTGCGCGTGGGTTTTCGTCCGTCTAATGGCATGCAGGTATTGGTGCGTGCTAGTGTCAGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCg                                                                                                                                                                      <  1:266192/251‑1 (MQ=255)                                                                      gcgcGTGGGTTTTCGTCCGTCTAATGGCATGCAGGTATTGGTGCGTGCTAGTGTCAGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTg                                                                                                                                                                                                                                            >  1:239777/1‑175 (MQ=255)                                                                         cgTGGGTTTTCGTCCGTCTAATGGCATGCAGGTATTGGTGCGTGCTAGTGTCAGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTA‑TACCTCTAAAACCGGGGCGGCAt                                                                                                                                                             <  1:663048/250‑1 (MQ=255)                                                                                 ttCGTCCGTCTAATGGCATGCAGGTATTGGTGCGTGCTAGTGTCAGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGat                                                                                                                                                     <  1:720239/251‑1 (MQ=255)                                                                                 ttCGTCCGTCTAATGGCATGCAGGTATTGGTGCGTGCTAGTGTCAGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGat                                                                                                                                                     <  1:527878/251‑1 (MQ=255)                                                                                        gtcTAATGGCATGCAGGTATTGGTGCGTGCTAGTGTCAGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGggc                                                                                                                                                                  <  1:592661/231‑1 (MQ=255)                                                                                                 cATGCAGGTATTGGTGCGTGCTAGTGTCAGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTAc                                                                                                                                     <  1:21226/251‑1 (MQ=255)                                                                                                 cATGCAGGTATTGGTGCGTGCTAGTGTCAGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTAc                                                                                                                                     <  1:729276/251‑1 (MQ=255)                                                                                                     cAGGTATTGGTGCGTGCTAGTGTCAGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTACAAcg                                                                                                                                 >  1:486284/1‑251 (MQ=255)                                                                                                     cAGGTATTGGTGCGTGCTAGTGTCAGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTACAAc                                                                                                                                  <  1:730368/250‑1 (MQ=255)                                                                                                      aGGTATTGGTGCGTGCTAGTGTCAGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCAttatta                                                                                                                                                                                  <  1:633873/201‑1 (MQ=255)                                                                                                       ggTATTGGTGCGTGCTAGTGTCAGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGc                                                                                                                                                                                         >  1:563050/1‑193 (MQ=255)                                                                                                       ggTATTGGTGCGTGCTAGTGTCAGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGatat                                                                                                                                                   >  1:470510/1‑231 (MQ=255)                                                                                                       ggTATTGGTGCGTGCTAGTGTCAGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTACAAcgt                                                                                                                                >  1:858525/1‑250 (MQ=255)                                                                                                       ggTATTGGTGCGTGCTAGTGTCAGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTACAAcgt                                                                                                                                >  1:844981/1‑250 (MQ=255)                                                                                                       ggTATTGGTGCGTGCTAGTGTCAGTTGGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCatt                                                                                                                                                                                      <  1:443250/196‑1 (MQ=255)                                                                                                        gTATTGGTGCGTGCTAGTGTCAGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTACAAcgtcg                                                                                                                              >  1:202318/1‑251 (MQ=255)                                                                                                        gTATTGGTGCGTGCTAGTGTCAGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTACAAcgt                                                                                                                                <  1:850751/249‑1 (MQ=255)                                                                                                              gtgcgtgcTAGTGTCAGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCatta                                                                                                                                                                                     >  1:729521/1‑190 (MQ=255)                                                                                                              gtgcgtgcTAGTGTCAGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTACAACGTCGTGATc                                                                                                                         <  1:8968/250‑1 (MQ=255)                                                                                                              gtgcgtgcTAGTGTCAGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTACAACGTCGTGATc                                                                                                                         <  1:602587/250‑1 (MQ=255)                                                                                                                  gtgcTAGTGTCAGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTACAACGTCGTGATCCGAGt                                                                                                                    >  1:402083/1‑251 (MQ=255)                                                                                                                       aGTGTCAGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCatt                                                                                                                                                                                      <  1:155394/180‑1 (MQ=255)                                                                                                                       aGTGTCAGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAAc                                                                                                                                                                        >  1:841483/1‑194 (MQ=255)                                                                                                                        gtgtCAGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCCACCGCACTTTAGTCAAGCGGTAGGCATTATTACct                                                                                                                                                                               <  1:221438/186‑1 (MQ=255)                                                                                                                             aGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTACAACGTCGTGATCCGAGTCTGAAAGTCat                                                                                                         >  1:126643/1‑251 (MQ=255)                                                                                                                             aGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTACAACGTCGTGATCCGAGTCTGAAAGTCat                                                                                                         >  1:378016/1‑251 (MQ=255)                                                                                                                                 tGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTACAACGTCGTGATCCGAGTCTGAAAg                                                                                                             <  1:803382/243‑1 (MQ=255)                                                                                                                                 tGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTACAACGTCGTGATCCGAGTCTGAAAGTCattatt                                                                                                     >  1:660023/1‑251 (MQ=255)                                                                                                                                  gTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTACAACGTCGTGATCCGAGTCTGAAAGTCAttattt                                                                                                    >  1:306896/1‑251 (MQ=255)                                                                                                                                        gCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCt                                                                                                                                                                                                                  <  1:9727/135‑1 (MQ=255)                                                                                                                                           gcgcgGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTCAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTACAACGTCGTGATCCGAGTCTGAAAGTCATTATTTATCCCACAg                                                                                           >  1:451072/1‑251 (MQ=255)                                                                                                                                            cgcgGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTACAACGTCGTGATCCGAGTCTGAAAGTCATTATTTATCCCACAGc                                                                                          <  1:607868/251‑1 (MQ=255)                                                                                                                                            cgcgGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTACAACGTCGTGATCCGAGTCTGAAAGTCATTATTTATCCCACAGc                                                                                          <  1:307281/251‑1 (MQ=255)                                                                                                                                            cgcgGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTACAACGTCGTGATCCGAGTCTGAAAGTCATTATTTATCCCACAGc                                                                                          <  1:593317/251‑1 (MQ=255)                                                                                                                                            cgcgGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTACAACGTCGTGATCCGAGTCTGAAAGTCATTATTTATCCCACAGc                                                                                          <  1:362143/251‑1 (MQ=255)                                                                                                                                            cgcgGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTACAACGTCGTGATCCGAGTCTGAAAGTCATTATTTATCCCACAGc                                                                                          <  1:733935/251‑1 (MQ=255)                                                                                                                                            cgcgGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTACAACGTCGTGATCCGAGTCTGAAAGTCATTATTTATCCCACAGc                                                                                          <  1:78800/251‑1 (MQ=255)                                                                                                                                                gTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGAGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTACAACGTCGTGATCCGAGTCTGAAAGTCATTATTTATCCCACAGCCGTg                                                                                      >  1:616077/1‑251 (MQ=255)                                                                                                                                                  gATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTACAACGTCGTGATCCGAGTCTGAAAGTCATTATTTATCCCACAGCCGTGCa                                                                                    >  1:816983/1‑251 (MQ=255)                                                                                                                                                   aTTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTACAACGTCGTGATCCGAGTCTGAAAGTCATTATTTATCCCACAGCCGTGCaa                                                                                   >  1:661832/1‑251 (MQ=255)                                                                                                                                                      aTCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTACAACGTCGTGATCCGAGTCTGAAAGTCATTATTTATCCCACAGCCGTGCAAGGt                                                                                <  1:831084/251‑1 (MQ=255)                                                                                                                                                           cTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTACAACGTCGTGATCCGAGTCTGAAAGTCATTATTTATCCCACAGCCGTGCAAGGTAAAGa                                                                           <  1:136367/251‑1 (MQ=255)                                                                                                                                                                 aTCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTAAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTACAACGTCGTGATCCGAGTCTGAAAGTCATTATTTATCCCACAGCCGTGCAAGGTAAAGAt                                                                          >  1:50734/1‑246 (MQ=255)                                                                                                                                                                        gTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTAc                                                                                                                                                                                 <  1:104123/136‑1 (MQ=255)                                                                                                                                                                        gTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTACAACGTCGTGATCCGAGTCTGAAAGTCATTATTTATcc                                                                                                >  1:132384/1‑217 (MQ=255)                                                                                                                                                                          aTGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTACAACGTCGTGATCCGAGTCTGAAAGTCATTATTTATCCCACAGCCGTGCAAGGTAAAGATGCGGCGGCTGAAAt                                                            <  1:834229/251‑1 (MQ=255)                                                                                                                                                                             cACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTACAAc                                                                                                                                  <  1:793359/178‑1 (MQ=255)                                                                                                                                                                               ccTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTACAACGTCGTGATCCGAGTCTGAAAGTCATTATTTATCCCACAGCCGTGCAAGGTAAAGATGCGGCGGCTGAAATCGca                                                        >  1:449709/1‑250 (MQ=255)                                                                                                                                                                                      ggCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTACAACGTCGTGATCCGAGTCTGAAAGTCATTATTTATCCCACAGCCGTGCAAGGTAAAGATg                                                                         >  1:890373/1‑226 (MQ=255)                                                                                                                                                                                            gggTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCgg                                                                                                                                                                                              >  1:454269/1‑103 (MQ=255)                                                                                                                                                                                            gggTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTACAACGTCGTGATCCGAGTCTGAAAGTCATTATTTATCCCACAGc                                                                                          >  1:769768/1‑203 (MQ=255)                                                                                                                                                                                                    gATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTACAACGTCGTGATCCGAGTCTGAAAGTCATTATTTATCCCACAGCCGTGCAAGGTAAAGATGCGGCGGCTGAAATCGCACAAATGATTGAAATCGCCAAc                                   >  1:170043/1‑250 (MQ=255)                                                                                                                                                                                                          gcaATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTACAACGTCGTGATCCGAGTCTGAAAGTCATTATTTATCCCACAGCCGTGCAAGGTAAAGATGCGGCGGCTGAAATCGCACAAATGATTGAAATCGCCAACCGGCGa                             >  1:544183/1‑250 (MQ=255)                                                                                                                                                                                                                        tGAAACTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTACAACGTCGTGATCCGAGTCTGAAAGTCATTATTTATCCCACAGCCGTGCAAGGTAAAGATGCGGCGGCTGAAATCGCACAAATGATTGAAATCGCCAACCGGCGACAAGAGGCGGATGTa              <  1:230694/251‑1 (MQ=255)                                                                                                                                                                                                                            aCTCAAATTAGCCGCAGAAGGATTGTTCGCTCAAAAGCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTAc                                                                                                                                     <  1:708123/128‑1 (MQ=255)                                                                                                                                                                                                                             cTCAAATTAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTACAACGTCGTGATCCGAGTCTGAAAGTCATTATTTATCCCACAGCCGTGCAAGGTAAAGATGCGGCGGCTGAAATCGCACAAATGATTGAAATCGCCAACCGGCGACAAGAGGCGGATGTATTAAt         <  1:785393/251‑1 (MQ=255)                                                                                                                                                                                                                                    tAGCGGCAGAAGGATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTACAACGTCGTGATCCGAGTCTGAAAGTCATTATTTATCCCACAGCCGTGCAAGGTAAAGATGCGGCGGCTGAAATCGCACAAATGATTGAAATCGCCAACCGGCGACAAGAGGCGGATGTATTAATCGTgggg  <  1:296098/251‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                               GCATTGGTATTTAAGTTTAAAAGACGAAAACGCCCAAGTGCGCTGCGCCATGTTCCGCATGAAAAATCTGCGCGTGGGTTTTCGTCCGTCTAATGGCATGCAGGTATTGGTGCGTGCTAGTGTCAGTTTGTATGAGCCGCGCGGTGATTATCAACTGATTATCGAAAGTATGCACCTTGCCGGCGAAGGGTTACTGATGCAGCAATTTGAAGCGCTGAAACTCAAATTAGCGGCAGAAGAATTGTTCGCTCAAAATCACAAGAAAAACCTACCGCACTTTAGTCAAGCGGTAGGCATTATTACCTCTAAAACCGGGGCGGCATTGCAGGATATTTTGCATATTCTACAACGTCGTGATCCGAGTCTGAAAGTCATTATTTATCCCACAGCCGTGCAAGGTAAAGATGCGGCGGCTGAAATCGCACAAATGATTGAAATCGCCAACCGGCGACAAGAGGCGGATGTATTAATCGTGGGG  >  1/1218292‑1218769

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑