Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,217,170	G→T	L67F (TTG→TTT)	unknown →	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,217,170	0	G	T	100.0%	233.2 / NA	82	L67F (TTG→TTT)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (44/38);  total (44/38)

GAAAAAATAATAACACTTATTCAACACATTGAAGGAATGTATATGAACAAATTTTGTTTACCTCAAAATATTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTGCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATGGTCGCCGTTATTAGGCAATTTATGGCGAAAATTCGACTTTATTCCGCAATGGCAACGTGATGACATCATGGTATCTTTCGCGCCACAAGGCGGTTCTGTCGGCAAACATTATGATGAATACGATGTCTTCCTGGTGCAGGCTTACGGTCATCGCCGTTGG  >  1/1216928‑1217410                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 gAAAAAATAATAACACTTATTCAACACATTGAAGGAATGTATATGAACAAATTTTGTTTACCTCAAAATATTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAg                                                                                                                                                                                                                                          >  1:494178/1‑251 (MQ=255)        taataaCACTTATTCAACACATTGAAGGAATGTATATGAACAAATTTTGTTTACCTCAAAATATTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGATATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTa                                                                                                                                                                                                                                   >  1:775284/1‑251 (MQ=255)          ataaCACTTATTCAACACATTGAAGGAATGTATATGAACAAATTTTGTTTACCTCAAAATATTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATc                                                                                                                                                                                                                                 <  1:686613/251‑1 (MQ=255)              cacTTATTCAACACATTGAAGGAATGTATATGAACAAATTTTGTTTACCTCAAAATATTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCAcc                                                                                                                                                                                                                                            >  1:247126/1‑236 (MQ=255)              cacTTATTCAACACATTGAAGGAATGTATATGAACAAATTTTGTTTACCTCAAAATATTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCaaa                                                                                                                                                                                                                             >  1:62520/1‑251 (MQ=255)              cacTTATTCAACACATTGAAGGAATGTATATGAACAAATTTTGTTTACCTCAAAATATTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCaaa                                                                                                                                                                                                                             >  1:655816/1‑251 (MQ=255)              cacTTATTCAACACATTGAAGGAATGTATATGAACAAATTTTGTTTACCTCAAAATATTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCaaa                                                                                                                                                                                                                             >  1:280283/1‑251 (MQ=255)                cTTATTCAACACATTGAAGGAATGTATATGAACAAATTTTGTTTACCTCAAAATATTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCAcc                                                                                                                                                                                                                                            <  1:667717/234‑1 (MQ=255)                cTTATTCAACACATTGAAGGAATGTATATGAACAAATTTTGTTTACCTCAAAATATTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAaaga                                                                                                                                                                                                                           <  1:179418/251‑1 (MQ=255)                   aTTCAACACATTGAAGGAATGTATATGAACAAATTTTGTTTACCTCAAAATATTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGt                                                                                                                                                                                                                                     >  1:814630/1‑238 (MQ=255)                   aTTCAACACATTGAAGGAATGTATATGAACAAATTTTGTTTACCTCAAAATATTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAagaaga                                                                                                                                                                                                                        >  1:124572/1‑251 (MQ=255)                          acaTTGAAGGAATGTATATGAACAAATTTTGTTTACCTCAAAATATTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGAt                                                                                                                                                                                                                 <  1:188117/251‑1 (MQ=255)                           caTTGAAGGAATGTATATGAACAAATTTTGTTTACCTCAAAATATTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCTCACCAGTCCGTTATCCAAAGAAGATTTTGATa                                                                                                                                                                                                                >  1:230567/1‑251 (MQ=255)                           caTTGAAGGAATGTATATGAACAAATTTTGTTTACCTCAAAATATTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATa                                                                                                                                                                                                                >  1:786124/1‑251 (MQ=255)                              tGAAGGAATGTATATGAACAAATTTTGTTTACCTCAAAATATTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAAcc                                                                                                                                                                                                             <  1:867776/251‑1 (MQ=255)                                   gAATGTATATGAACAAATTTTGTTTACCTCAAAATATTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAaaga                                                                                                                                                                                                                           <  1:603394/232‑1 (MQ=255)                                   gAATGTATATGAACAAATTTTGTTTACCTCAAAATATTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAAcctgc                                                                                                                                                                                                          >  1:775984/1‑249 (MQ=255)                                    aaTGTATATGAACAAATTTTGTTTACCTCAAAATATTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAAcctgc                                                                                                                                                                                                          >  1:540774/1‑248 (MQ=255)                                        tataTGAACAAATTTTGTTTACCTCAAAATATTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAaagaag                                                                                                                                                                                                                         <  1:585057/229‑1 (MQ=255)                                                cAAATTTTGTTTACCTCAAAATATTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCgg                                                                                                                                                                                           >  1:131747/1‑251 (MQ=255)                                                   aTTTTGTTTACCTCAAAATATTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCAc                                                                                                                                                                                                                                             <  1:448871/198‑1 (MQ=255)                                                             ccTCAAAATATTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTATTTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGa                                                                                                                                                                              >  1:592540/1‑251 (MQ=255)                                                              cTCAAAATATTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGat                                                                                                                                                                             <  1:266671/251‑1 (MQ=255)                                                                  aaaTATTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTcc                                                                                                                                                                                                                                       <  1:581922/189‑1 (MQ=255)                                                                  aaaTATTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTa                                                                                                                                                                                                                                   <  1:802736/193‑1 (MQ=255)                                                                    atatTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGatat                                                                                                                                                                           >  1:190028/1‑247 (MQ=255)                                                                    atatTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAg                                                                                                                                                                       <  1:487313/251‑1 (MQ=255)                                                                    atatTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAg                                                                                                                                                                       >  1:63059/1‑251 (MQ=255)                                                                     tatTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGc                                                                                                                                                                      <  1:538360/251‑1 (MQ=255)                                                                          cacCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATgg                                                                                                                                                                 <  1:649956/251‑1 (MQ=255)                                                                              ggAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATGGTCg                                                                                                                                                              >  1:624849/1‑250 (MQ=255)                                                                              ggAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTCTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCggatt                                                                                                                                                                                        >  1:841637/1‑221 (MQ=255)                                                                                      ttttACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGc                                                                                                                                                                      <  1:161951/234‑1 (MQ=255)                                                                                      ttttACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATGGTCGCCGttatta                                                                                                                                                     <  1:371386/251‑1 (MQ=255)                                                                                        ttACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATGGTCGCCGTTATTAgg                                                                                                                                                   <  1:496191/251‑1 (MQ=255)                                                                                               aTTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATGGTCGCCGTTATTAGGCAATTTa                                                                                                                                            <  1:481882/251‑1 (MQ=255)                                                                                                     ggCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATGGTCGCCGTTATTAGGCAATTTATGGCGa                                                                                                                                      >  1:105008/1‑251 (MQ=255)                                                                                                         gAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAaaga                                                                                                                                                                                                                           <  1:640380/162‑1 (MQ=255)                                                                                                         gAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATGGTCGCCGTTATTAGGCAATTTATGGCGAAAAt                                                                                                                                  >  1:26158/1‑251 (MQ=255)                                                                                                          aaaaaaCCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAaaga                                                                                                                                                                                                                           <  1:238403/161‑1 (MQ=255)                                                                                                              aaCCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATGGTCGCCGTTATTAGGCAATTTATGGCGAAAATTCGAc                                                                                                                             <  1:450028/251‑1 (MQ=255)                                                                                                                  gTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTa                                                                                                                                                                                                                                   >  1:520736/1‑145 (MQ=255)                                                                                                                   ttattaATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATGGTCGCCGTTATTAGGCAATTTATGGCGAAAATTCGACTTTAt                                                                                                                        >  1:830038/1‑251 (MQ=255)                                                                                                                    tattaATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGc                                                                                                                                                                      >  1:180468/1‑204 (MQ=255)                                                                                                                     attaATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATGGTCGCCGTTATTAGGCAATTTATGGCGAAAATTCGACTTTATTc                                                                                                                      <  1:491094/251‑1 (MQ=255)                                                                                                                                   gTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATGGTCGCCGTTATTAGGCAATTTATGGCGAAAATTCGACTTTATTCCGCAATGGCAACGt                                                                                                        >  1:437176/1‑251 (MQ=255)                                                                                                                                            aaaTTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGa                                                                                                                                                                        <  1:489982/178‑1 (MQ=255)                                                                                                                                            aaaTTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATGGTCGCCGTTATTAGGCAATTTATGGCGAAAATTCGACTTTATTCCGCAATGGCAACGTGATGAc                                                                                                  <  1:544154/248‑1 (MQ=255)                                                                                                                                            aaaTTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATGGTCGCCGTTATTAGGCAATTTATGGCGAAAATTCGACTTTATTCCGCAATGGCAACGTGATGAc                                                                                                  <  1:441512/248‑1 (MQ=255)                                                                                                                                               ttATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATGGTCGCCGTTATTAGGCAATTTATGGCGAAAATTCGACTTTATTCCGCAATGGc                                                                                                             >  1:245459/1‑234 (MQ=255)                                                                                                                                               ttATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATGGTCGCCGTTATTAGGCAATTTATGGCGAAAATTCGACTTTATTCCGCAATGGCAACGTGATGACATCATg                                                                                            >  1:609734/1‑251 (MQ=255)                                                                                                                                                     gTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGc                                                                                                                                                                      >  1:224728/1‑171 (MQ=255)                                                                                                                                                     gTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATGGTCGCCGTTATTAGGCAATTTATGGCGAAAATTCGACTTTAt                                                                                                                        >  1:88293/1‑217 (MQ=255)                                                                                                                                                        tGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATGGTCGCCGTTATTAGGCAATTTATGGCGAAAATTCGACTTTATTCCGCAATGGCAACGTGATGAc                                                                                                  >  1:157940/1‑236 (MQ=255)                                                                                                                                                        tGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATGGTCGCCGTTATTAGGCAATTTATGGCGAAAATTCGACTTTATTCCGCAATGGCAACGTGATGACATCATGGTATCttt                                                                                    >  1:670956/1‑250 (MQ=255)                                                                                                                                                        tGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATGGTCGCCGTTATTAGGCAATTTATGGCGAAAATTCGACTTTATTCCGCAATGGCAACGTGATGACATCATGGTATCttt                                                                                    >  1:845540/1‑250 (MQ=255)                                                                                                                                                         gCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTg                                                                                                                                                                                   <  1:346594/154‑1 (MQ=255)                                                                                                                                                         gCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATGGTCGCCGTTATTAGGCAATTTATGGCGAAAATTCGACTTTATTCCGCAATGGCAACGTGATGACATCATGGt                                                                                          <  1:556710/243‑1 (MQ=255)                                                                                                                                                         gCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATGGTCGCCGTTATTAGGCAATTTATGGCGAAAATTCGACTTTATTCCGCAATGGCAACGTGATGACATCATGGTATCTTTcg                                                                                  >  1:166742/1‑251 (MQ=255)                                                                                                                                                         gCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATGGTCGCCGTTATTAGGCAATTTATGGCGAAAATTCGACTTTATTCCGCAATGGCAACGTGATGACATCATGGTATCTTTcg                                                                                  >  1:561601/1‑251 (MQ=255)                                                                                                                                                          cTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATGGTCGCCGTTATTAGGCAATTTATGGCGAAAATTCGACTTTATTCCGCAATGGCAACGTGATGACATCATGGTATCTTTcg                                                                                  >  1:481017/1‑250 (MQ=255)                                                                                                                                                                     cgACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGc                                                                                                                                                                      >  1:588924/1‑155 (MQ=255)                                                                                                                                                                         aTCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATGGTCGCCGTTATTAGGCAATTTATGGCGAAAATTCGACTTTATTCCGCAATGGCAACGTGATGACATCATGGTATCTTTCGCGCCACAAGGCGGTTc                                                                  <  1:573257/251‑1 (MQ=255)                                                                                                                                                                              cGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATGGTCGCCGTTATTAGGCAATTTATGGCGAAAATTCGACTTTATTCCGCAATGGCAACGTGATGACATCATGGTATCTTTCGCGCCACAAGGCGGTTCTGTCg                                                             <  1:656540/251‑1 (MQ=255)                                                                                                                                                                                            cGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATGGTCGCCGTTATTAGGCAATTTATGGCGAAAATTCGACTTTATTCCGCAATGGCAACGTGATGACATCATGGt                                                                                          >  1:492620/1‑208 (MQ=255)                                                                                                                                                                                            cGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATGGTCGCCGTTATTAGGCAATTTATGGCGAAAATTCGACTTTATTCCGCAATGGCAACGTGATGACATCATGGTATCTTTCGCGCCACAAGGCGGTTCTGTCGGCAAACATTatgat                                               <  1:520838/251‑1 (MQ=255)                                                                                                                                                                                            cGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATGGTCGCCGTTATTAGGCAATTTATGGCGAAAATTCGACTTTATTCCGCAATGGCAACGTGATGACATCATGGTATCTTTCGCGCCACAAGGCGGTTCTGTCGGCAAACATTatgat                                               <  1:333609/251‑1 (MQ=255)                                                                                                                                                                                            cGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATGGTCGCCGTTATTAGGCAATTTATGGCGAAAATTCGACTTTATTCCGCAATGGCAACGTGATGACATCATGGTATCTTTCGCGCCACAAGGCGGTTCTGTCGGCAAACATTatga                                                <  1:862111/250‑1 (MQ=255)                                                                                                                                                                                              gaagaaGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATGGTCGCCGTTATTAGGCAATTTATGGCGAAAATTCGACTTTATTCCGCAATGGCaa                                                                                                           >  1:684085/1‑189 (MQ=255)                                                                                                                                                                                                  aagTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATGGTCGCCGTTATTAGGCAATTTATGGCGAAAATTCGACTTTATTCCGCAATGGCAACGTGATGACATCATGGTATCTTTCGCGCCACAAGGCGGTTCTGTCGGCAAACATTATGATGAATa                                          >  1:201014/1‑250 (MQ=255)                                                                                                                                                                                                             cgcTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATGGTCGCCGTTATTAGGCAATTTATGGCGAAAATTCGACTTTATTCCGCAATGGCAACGTGATGACATCATGGTATCTTTCGCGCCACAAGGCGGTTCTGTCGGCAAACATTATGATGAATACGATGt                                    >  1:837757/1‑245 (MQ=255)                                                                                                                                                                                                               cTTGATCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATGGTCGCCGTTATTAGGCAATTTATGGCGAAAATTCGACTTTATTCCGCAATGGCAACGTGATGACATCATGGTATCTTTCGCGCCACAAGGCGGTTCTGTCGGCAAACATTATGATGAATACGATGTCTTCCTg                             <  1:297354/250‑1 (MQ=255)                                                                                                                                                                                                                   aTCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCaa                                                                                                                                                                    >  1:598274/1‑111 (MQ=255)                                                                                                                                                                                                                   aTCAAGCAACATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATGGTCGCCGTTATTAGGCAATTTATGGCGAAAATTCGACTTTATTCCGCAATGGCAACGTGATGACATCATGGTATCTTTCGCGCCACAAGGCGGTTCTGTCGGCAAACATTATGATGAATACGATGTCTTCCTGGTGCa                        >  1:265471/1‑251 (MQ=255)                                                                                                                                                                                                                            cATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATGGTCGCCGTTATTAGGCAATTTATGGCGAAAATTCGACTTTATTCCGCAATGGCAACGTGATGACATCATGGTATCTTTCGCGCCACAAGGCGGTTCTGTCGGCAAACATTATGATGAATACGATGTCTTCCTGGTGCAGGCTTACgg               <  1:195138/251‑1 (MQ=255)                                                                                                                                                                                                                            cATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATGGTCGCCGTTATTAGGCAATTTATGGCGAAAATTCGACTTTATTCCGCAATGGCAACGTGATGACATCATGGTATCTTTCGCGCCACAAGGCGGTTCTGTCGGCAAACATTATGATGAATACGATGTCTTCCTGGTGCAGGCTTACgg               <  1:86735/251‑1 (MQ=255)                                                                                                                                                                                                                            cATTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATGGTCGCCGTTATTAGGCAATTTATGGCGAAAATTCGACTTTATTCCGCAATGGCAACGTGATGACATCATGGTATCTTTCGCGCCACAAGGCGGTTCTGTCGGCAAACATTATGATGAATACGATGTCTTCCTGGTGCAGGCTTACgg               <  1:547701/251‑1 (MQ=255)                                                                                                                                                                                                                             aTTCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATGGTCGCCGTTATTAGGCAATTTATGGCGAAAATTCGACTTTATTCCGCAATGGCAACGTGATGACATCATGGTATCTTTCGCGCCACAAGGCGGTTCTGTCGGCAAACATTATGATGAATACGATGTCTTCCTGGTGCAGGCTTACGGt              >  1:277574/1‑251 (MQ=255)                                                                                                                                                                                                                              ttCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATGGTCGCCGTTATTAGGCAATTTATGGCGAAAATTCGACTTTATTCCGCAATGGCAACGTGATGACATCATGGTATCTTTCGCGCCACAAGGCGGTTCTGTCGGCAAACATTATGATGAATACGATGTCTTCCTGGTGCAGGCTTACGGTc             <  1:483071/251‑1 (MQ=255)                                                                                                                                                                                                                              ttCCGATGACAATTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATGGTCGCCGTTATTAGGCAATTTATGGCGAAAATTCGACTTTATTCCGCAATGGCAACGTGATGACATCATGGTATCTTTCGCGCCACAAGGCGGTTCTGTCGGCAAACATTATGATGAATACGATGTCTTCCTGGTGCAGGCTTACGGTc             <  1:842705/251‑1 (MQ=255)                                                                                                                                                                                                                                         aTTGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATGGTCGCCGTTATTAGGCAATTTATGGCGAAAATTCGACTTTATTCCGCAATGGCAACGTGATGACATCATGGTATCTTTCGCGCCACAAGGCGGTTCTGTCGGCAAACATTATGATGAATACGATGTCTTCCTGGTGCAGGCTTACGGTCATCGCCGTTgg  >  1:490608/1‑251 (MQ=255)                                                                                                                                                                                                                                           tGGGAATTTCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGc                                                                                                                                                                      >  1:46749/1‑85 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                 GAAAAAATAATAACACTTATTCAACACATTGAAGGAATGTATATGAACAAATTTTGTTTACCTCAAAATATTACACCGGAAATCTTTTTACGCGATTACTGGCAGAAAAAACCGTTATTAATTCGACGCGGTTTACCAGAAATTATCGGTATGCTTGAACCACGCGACATCACCGAATTAGCCAAAACGGAAGAAGTGGTCGCGCGCTTGATCAAGCAACATTCCGATGACAATTGGGAATTGCGCACCAGTCCGTTATCCAAAGAAGATTTTGATAACCTGCCTGAAAAATGGTCGGTTTTAGTGCAAGATATGGAGCAATGGTCGCCGTTATTAGGCAATTTATGGCGAAAATTCGACTTTATTCCGCAATGGCAACGTGATGACATCATGGTATCTTTCGCGCCACAAGGCGGTTCTGTCGGCAAACATTATGATGAATACGATGTCTTCCTGGTGCAGGCTTACGGTCATCGCCGTTGG  >  1/1216928‑1217410

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑