Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,214,097	C→T	V100I (GTT→ATT)	unknown ←	Guanylate kinase (EC 2.7.4.8)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,214,097	0	C	T	100.0%	215.2 / NA	79	V100I (GTT→ATT)	unknown	Guanylate kinase (EC 2.7.4.8)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (46/33);  total (46/33)

CCTGCAAATCCACAAGCGCCTGTTCAAAATTATCGTTGACGATCACATAATCATATTCATTGTAATGAGAAATTTCGCTCATCGCTTTCGCCATTCTGTCGGCAATCACGTCCGCACTGTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAACATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCAAATTCCCCTTGAGAAACAAAATAATAATGCACGCCTTCTTGTTCGCCCGGGCGCGACTGTCTGGTGGTGTGGGAAACGGATACCATCATTTTATGGTTATTATTTTGTTTTAACAATGCGG  >  1/1213851‑1214330                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                          ccTGCAAATCCACAAGCGCCTGTTCAAAATTATCGTTGACGATCACATAATCATATTCATTGTAATGAGAAATTTCGCTCATCGCTTTCGCCATTCTGTCGGCAATCACGTCCGCACTGTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATc                                                                                                                                                                                                                                        <  1:280198/250‑1 (MQ=255)     cAAATCCACAAGCGCCTGTTCAAAATTATCGTTGACGATCACATAATCATATTCATTGTAATGAGAAATTTCGCTCATCGCTTTCGCCATTCTGTCGGCAATCACGTCCGCACTGTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGc                                                                                                                                                                                                                                   <  1:335064/251‑1 (MQ=255)                      gTTCAAAATTATCGTTGACGATCACATAATCATATTCATTGTAATGAGAAATTTCGCTCATCGCTTTCGCCATTCTGTCGGCAATCACGTCCGCACTGTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCGATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAAtttt                                                                                                                                                                                                                    >  1:881663/1‑249 (MQ=255)                      gTTCAAAATTATCGTTGACGATCACATAATCATATTCATTGTAATGAGAAATTTCGCTCATCGCTTTCGCCATTCTGTCGGCAATCACGTCCGCACTGTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGc                                                                                                                                                                                                                            >  1:276975/1‑241 (MQ=255)                                   gTTGACGATCACATAATCATATTCATTGTAATGAGAAATTTCGCTCATCGCTTTCGCCATTCTGTCGGCAATCACGTCCGCACTGTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATa                                                                                                                                                                                                     >  1:404801/1‑251 (MQ=255)                                    ttGACGATCACATAATCATATTCATTGTAATGAGAAATTTCGCTCATCGCTTTCGCCATTCTGTCGGCAATCACGTCCGCACTGTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATaa                                                                                                                                                                                                    >  1:241004/1‑251 (MQ=255)                                           tCACATAATCATATTCATTGTAATGAGAAATTTCGCTCATCGCTTTCGCCATTCTGTCGGCAATCACGTCCGCACTGTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTg                                                                                                                                                                                             >  1:377365/1‑251 (MQ=255)                                            cacaTAATCATATTCATTGTAATGAGAAATTTCGCTCATCGCTTTCGCCATTCTGTCGGCAATCACGTCCGCACTGTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGa                                                                                                                                                                                                 <  1:63033/246‑1 (MQ=255)                                            cacaTAATCATATTCATTGTAATGAGAAATTTCGCTCATCGCTTTCGCCATTCTGTCGGCAATCACGTCCGCACTGTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTg                                                                                                                                                                                             >  1:192840/1‑250 (MQ=255)                                             acaTAATCATATTCATTGTAATGAGAAATTTCGCTCATCGCTTTCGCCATTCTGTCGGCAATCACGTCCGCACTGTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGc                                                                                                                                                                                                                            >  1:159947/1‑218 (MQ=255)                                              caTAATCATATTCATTGTAATGAGAAATTTCGCTCATCGCTTTCGCCATTCTGTCGGCAATCACGTCCGCACTGTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTttc                                                                                                                                                                                                                   >  1:146312/1‑226 (MQ=255)                                              caTAATCATATTCATTGTAATGAGAAATTTCGCTCATCGCTTTCGCCATTCTGTCGGCAATCACGTCCGCACTGTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGa                                                                                                                                                                                                 <  1:259100/244‑1 (MQ=255)                                              caTAATCATATTCATTGTAATGAGAAATTTCGCTCATCGCTTTCGCCATTCTGTCGGCAATCACGTCCGCACTGTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACg                                                                                                                                                                                               >  1:751721/1‑246 (MQ=255)                                              caTAATCATATTCATTGTAATGAGAAATTTCGCTCATCGCTTTCGCCATTCTGTCGGCAATCACGTCCGCACTGTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCg                                                                                                                                                                                          >  1:456359/1‑251 (MQ=255)                                                  aTCATATTCATTGTAATGAGAAATTTCGCTCATCGCTTTCGCCATTCTGTCGGCAATCACGTCCGCACTGTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGCATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGtaat                                                                                                                                                                                      >  1:369821/1‑251 (MQ=255)                                                  aTCATATTCATTGTAATGAGAAATTTCGCTCATCGCTTTCGCCATTCTGTCGGCAATCACGTCCGCACTGTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTttct                                                                                                                                                                                                                  >  1:411676/1‑223 (MQ=255)                                                  aTCATATTCATTGTAATGAGAAATTTCGCTCATCGCTTTCGCCATTCTGTCGGCAATCACGTCCGCACTGTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGtaat                                                                                                                                                                                      >  1:702921/1‑251 (MQ=255)                                                  aTCATATTCATTGTAATGAGAAATTTCGCTCATCGCTTTCGCCATTCTGTCGGCAATCACGTCCGCACTGTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGtaat                                                                                                                                                                                      >  1:702185/1‑251 (MQ=255)                                                  aTCATATTCATTGTAATGAGAAATTTCGCTCATCGCTTTCGCCATTCTGTCGGCAATCACGTCCGCACTGTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGtaat                                                                                                                                                                                      >  1:850102/1‑251 (MQ=255)                                                  aTCATATTCATTGTAATGAGAAATTTCGCTCATCGCTTTCGCCATTCTGTCGGCAATCACGTCCGCACTGTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGtaat                                                                                                                                                                                      >  1:357507/1‑251 (MQ=255)                                                      tatTCATTGTAATGAGAAATTTCGCTCATCGCTTTCGCCATTCTGTCGGCAATCACGTCCGCACTGTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAAtt                                                                                                                                                                                  >  1:514123/1‑251 (MQ=255)                                                         tCATTGTAATGAGAAATTTCGCTCATCGCTTTCGCCATTCTGTCGGCAATCACGTCCGCACTGTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACg                                                                                                                                                                                               <  1:300151/235‑1 (MQ=255)                                                                                           ccATTCTGTCGGCAATCACGTCCGCACTGTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGAc                                                                                                                                                                                                <  1:760983/200‑1 (MQ=255)                                                                                             aTTCTGTCGGCAATCACGTCCGCACTGTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTg                                                                                                                                           >  1:462286/1‑251 (MQ=255)                                                                                                  gTCGGCAATCACGTCCGCACTGTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGa                                                                                                                                                                                                 <  1:682046/192‑1 (MQ=255)                                                                                                        aaTCACGTCCGCACTGTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAgg                                                                                                                                >  1:20251/1‑251 (MQ=255)                                                                                                             cGTCCGCACTGTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAAt                                                                                                                                                                                                             >  1:472503/1‑169 (MQ=255)                                                                                                             cGTCCGCACTGTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCa                                                                                                                           >  1:833043/1‑251 (MQ=255)                                                                                                             cGTCCGCACTGTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCa                                                                                                                           >  1:316639/1‑251 (MQ=255)                                                                                                             cGTCCGCACTGTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCa                                                                                                                           >  1:140239/1‑251 (MQ=255)                                                                                                                  gCACTGTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAg                                                                                                                                                                                                           >  1:613946/1‑166 (MQ=255)                                                                                                                       gTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGc                                                                                                                                                                                                                                   >  1:562095/1‑137 (MQ=255)                                                                                                                       gTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTttcttc                                                                                                                                                                                                                <  1:830134/156‑1 (MQ=255)                                                                                                                       gTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAg                                                                                                                                                                                                           <  1:315550/161‑1 (MQ=255)                                                                                                                       gTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACg                                                                                                                                                                                               <  1:220171/173‑1 (MQ=255)                                                                                                                       gTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCaaaaa                                                                                                                                                    <  1:501876/216‑1 (MQ=255)                                                                                                                       gTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAgg                                                                                                                                >  1:851106/1‑236 (MQ=255)                                                                                                                       gTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCAAATTc                                                                                                                      <  1:500918/246‑1 (MQ=255)                                                                                                                       gTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCAAATTCCCCtt                                                                                                                 >  1:212118/1‑251 (MQ=255)                                                                                                                       gTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCAAATTCCCCtt                                                                                                                 >  1:14647/1‑251 (MQ=255)                                                                                                                       gTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCAAATTCCCCtt                                                                                                                 >  1:45452/1‑251 (MQ=255)                                                                                                                       gTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCAAATTCCCCtt                                                                                                                 >  1:191421/1‑251 (MQ=255)                                                                                                                       gTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCAAATTCCCCtt                                                                                                                 >  1:626111/1‑251 (MQ=255)                                                                                                                       gTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCAAATTCCCCtt                                                                                                                 >  1:325852/1‑251 (MQ=255)                                                                                                                       gTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCAAATTCCCCtt                                                                                                                 >  1:374117/1‑251 (MQ=255)                                                                                                                                       aTTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCAAATTCCCCTTGAGAAACAAaataat                                                                                                  <  1:700274/250‑1 (MQ=255)                                                                                                                                                      aaTTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCAAATTCCCCTTGAGAAACAAAATAATAATGCACGCCTTCTTg                                                                                  <  1:276172/251‑1 (MQ=255)                                                                                                                                                        ttCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCAAATTCCCCTTGAGAAACAAAATAATAATGCACGCCTTCTTGtt                                                                                <  1:328416/251‑1 (MQ=255)                                                                                                                                                         tCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCAAATTCCCCTTGAGAAACAAAATAATAATGCACGCCTTCTTGTTc                                                                               <  1:703992/251‑1 (MQ=255)                                                                                                                                                            tCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCAAATTCCCCTTGAGAAACAAAATAATAATGCACGCCTTCTTGTTCGc                                                                             >  1:109839/1‑250 (MQ=255)                                                                                                                                                                acgacgGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCAAATTCCCCTTGAGAAACAAAATAATAATGCACGCCTTCTTGTTCGCCCggg                                                                        <  1:781736/251‑1 (MQ=255)                                                                                                                                                                      gcggTAAAACAAAAATCCTCTAAACATTCGGCATTTTTTTGCGAATTTGTTGTGCGCCCTGCAAATCAATATCAAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCAAAt                                                                                                                        <  1:655372/197‑1 (MQ=255)                                                                                                                                                                      gcggTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGAGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCAAATTCCCCTTGAGAAACAAAATAATAATGCACGCCTTCTTGt                                                                                 >  1:565586/1‑236 (MQ=255)                                                                                                                                                                       cggTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCAAATTCCCCTTGAGAAACAAAATAATAATGCACGCCTTCTTGTTCGCCCGGGCGCGAc                                                                  <  1:739592/250‑1 (MQ=255)                                                                                                                                                                             aaCAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGAc                                                                                                                                                                                                >  1:117263/1‑118 (MQ=255)                                                                                                                                                                               cAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCAAATTCCCCTTGAGAAACAAaataataa                                                                                                >  1:306318/1‑212 (MQ=255)                                                                                                                                                                               cAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCAAATTCCCCTTGAGAAACAAaataat                                                                                                  <  1:815482/210‑1 (MQ=255)                                                                                                                                                                                aaaaaTACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCAAATTCCCCTTGAGAAACAAAATAATAATGCACGCCTTCTTGTTCGCCCGGGCGCGACTGTCtggtgg                                                        <  1:251271/251‑1 (MQ=255)                                                                                                                                                                                aaaaaTACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCAAATTCCCCTTGAGAAACAAAATAATAATGCACGCCTTCTTGTTCGCCCGGGCGCGACTGTCtggtgg                                                        <  1:322949/251‑1 (MQ=255)                                                                                                                                                                                 aaaaTACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGAt                                                                                                                                     >  1:733849/1‑173 (MQ=255)                                                                                                                                                                                      aCTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTGAGGTTTCAAATTCCCCTTGAGAAACAAAATAATAATGCACGCCTTCTTGtt                                                                                >  1:435036/1‑221 (MQ=255)                                                                                                                                                                                       ctctTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCAAATTCCCCTTGAGAAACAAAATAATAATGCACGCCTTCTTGTTCGCCCGGGCGCGACTGTCTGGTGGTGTGGGa                                                 >  1:637358/1‑251 (MQ=255)                                                                                                                                                                                            aaCATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCAAATTCCCCTTGAGAAACAAAATAATAATGCACGCCTTCTTGTTCGCCCGGGCGCGACTGTCTGGTGGTGTGGGAAACgg                                            <  1:292871/251‑1 (MQ=255)                                                                                                                                                                                                        ttttttGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCAAATTCCCCTTGAGAAACAAAATAATAATGCACGCCTTCTTGTTCGCCCGGGCGCGACTGTCTGGTGGTGTGGGAAACGGATACCATCAttt                                <  1:250473/251‑1 (MQ=255)                                                                                                                                                                                                            ttGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCAAATTCCCCTTGAGAAACAAAATAATAATGCACGCCTTCTTGTTCGCCCGGGCGCGACTGTCTGGTGGTGTGGGAAACGGATACCATCATTTTATg                            <  1:395346/251‑1 (MQ=255)                                                                                                                                                                                                             tGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCAAATTCCCCTTGAGAAACAAAATAATAATGCACGCCTTCTTGTTCGCCCGGGCGCGACTGTCTGGTGGTGTGGGAAACGGATACCATCATTTTATgg                           <  1:335315/251‑1 (MQ=255)                                                                                                                                                                                                             tGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCAAATTCCCCTTGAGAAACAAAATAATAATGCACGCCTTCTTGTTCGCCCGGGCGCGACTGTCTGGTGGTGTGGGAAACGGATACCATCATTTTATgg                           <  1:814511/251‑1 (MQ=255)                                                                                                                                                                                                                 aaTTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCAAATTCCCCTTGAGAAACAAAATAATAATGCACGCCTTCTTGTTCGCCCGGGCGCGACTGTCTGGTGGTGTGGGAAACGGATACCATCATTTTATGGttat                       >  1:665618/1‑251 (MQ=255)                                                                                                                                                                                                                 aaTTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCAAATTCCCCTTGAGAAACAAAATAATAATGCACGCCTTCTTGTTCGCCCGGGCGCGACTGTCTGGTGGTGTGGGAAACGGATACCATCATTTTATGGttat                       >  1:258202/1‑251 (MQ=255)                                                                                                                                                                                                                           gcgcCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCAAATTCCCCTTGAGAAACAAAATAATAATGCACGCCTTCTTGTTCGCCCGGGCGCGACTGTCTGGTGGTGTGGGAAACgg                                            <  1:113006/220‑1 (MQ=255)                                                                                                                                                                                                                            cgcCCTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCAAATTCCCCTTGAGAAACAAAATAATAATGCACGCCTTCTTGTTCGCCCGGGCGCGACTGTCTGGTGGTGTGGGAAACGGATACCATCATTTTATGGtt                         <  1:46322/238‑1 (MQ=255)                                                                                                                                                                                                                                cTGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCAAATTCCCCTTGAGAAACAAaataataa                                                                                                <  1:847275/163‑1 (MQ=255)                                                                                                                                                                                                                                 tGCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCAAATTCCCCTTGAGAAACAAAATAATAATGCACGCCTTCTTGTTCGCCCGGGCGCGACTGTCTGGTGGTGTGGGAAACGGATACCATCATTTTATGGTTATTATTTTGTTTTAACaa       <  1:291669/251‑1 (MQ=255)                                                                                                                                                                                                                                  gCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCAAATTCCCCTTGAGAAACAAAATAATAATGCACGCCTTCTTGTTCGCCCGGGCGCGACTGTCTGGTGGTGTGGGAAACGGATAcc                                       >  1:873095/1‑218 (MQ=255)                                                                                                                                                                                                                                  gCCAATCAATATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCAAATTCCCCTTGAGAAACAAAATAATAATGCACGCCTTCTTGTTCGCCCGGGCGCGACTGTCTGGTGGTGTGGGAAACGGATACCATCATTTTATGGTTATTATTTTGTTTTAACAAt      >  1:744590/1‑251 (MQ=255)                                                                                                                                                                                                                                      atcaatATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCAAATTCCCCTTGAGAAACAAAATAATAATGCACGCCTTCTTGTTCGCCCGGGCGCGACTGTCTGGTGGTGTGGGAAACGGATACCATCATTTTATGGTTATTATTTTGTTTTAACAATgcgg  <  1:637949/251‑1 (MQ=255)                                                                                                                                                                                                                                       tcaatATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCAAATTCCCCTTGAGAAACAAAATAATAATGCACGCCTTCTTGTTCGCCCGGGCGCGACTGTCtggtgg                                                        >  1:507454/1‑196 (MQ=255)                                                                                                                                                                                                                                       tcaatATCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCAAATTCCCCTTGAGAAACAAAATAATAATGCACGCCTTCTTGTTCGCCCGGGCGCGACTGTCTGGTGGTGTGGGAAACGGATACCATCATTTTATGGTTATTATTTTGTTTTAACa        <  1:272949/244‑1 (MQ=255)                                                                                                                                                                                                                                            atCCAAAAAAATATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCAAATTCCCCTTGAGAAACAAAATAATAATGCACGCCTTCTTGTTCGCCCGGGCGCGACTGTCTGGTGGTGTGGGAAACGGATACCATCATTTTATGGTtattat                    >  1:385669/1‑227 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                          CCTGCAAATCCACAAGCGCCTGTTCAAAATTATCGTTGACGATCACATAATCATATTCATTGTAATGAGAAATTTCGCTCATCGCTTTCGCCATTCTGTCGGCAATCACGTCCGCACTGTCCTGACCGCGTCCGATTAAGCGTCTTTCCAATTCATCCAACGACGGCGGTAAAACAAAAATACTCTTAACATTCGGCACTTTTTTGCGAATTTGTTGTGCGCCCTGCCAATCAATATCCAAAAAAACATCAATGCCTTTAGCTAAATTTTCTTCAATAGCGAATAAAGACGTGCCGTAATAATTGCCGCCAAAGACTTTCGCGTATTCCAAAAAGGCATTTTGTTCGATTAAGGTTTCAAATTCCCCTTGAGAAACAAAATAATAATGCACGCCTTCTTGTTCGCCCGGGCGCGACTGTCTGGTGGTGTGGGAAACGGATACCATCATTTTATGGTTATTATTTTGTTTTAACAATGCGG  >  1/1213851‑1214330

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑