| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 1,210,193 | T→C | D357G (GAC→GGC) | unknown ← | ATP‑dependent DNA helicase RecG (EC 3.6.4.12) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 1,210,193 | 0 | T | C | 98.7% | 220.2 / NA | 78 | D357G (GAC→GGC) | unknown | ATP‑dependent DNA helicase RecG (EC 3.6.4.12) |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base C (43/34); total (44/34) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
GTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATACACGCCTGCCTGATTACCTTTTTCCCGCAGCATGAGTCGTTGGTGCACGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGTCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTCGGCGCCATCAATGCCACCTGTTTACCGTTATCAATGGCGAGCAGTGCCGCTAACGCCGCCACTAAGGTTTTACCGGAGCCGACATCCCCCTGCACCAGACGCATCATCGGGTAAGGTTGCGCCACATCCCGTTCAATAT > 1/1209950‑1210427 | gTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATACACGCCCGCCTTATTACCTTTTTTCCGCAGCATGAGTCGTTGGGGCACGCCGCAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTTCACCTCTTCCTGGAATAAAGCGTGCGTTCCCACCCCCATTTGTACCCCACCGCCTTTTTTTTTTTCTAGCTCGGCAACCCCTTGTTTGTCTTTGa > 1:232035/1‑250 (MQ=255) aGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATACACGCCTGCCTGATTACCTTTTTCCCGCAGCATGAGTCGTTGGGGCACGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAAAGCGTGCGGTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTg > 1:140131/1‑250 (MQ=255) gCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATACACGCCTGCCTGATTACCTTTTTCCCGCAGCCTGAGTCGTTGGTGCACGCCGAAACGGGGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAAATCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTTTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTTCTgc > 1:872762/1‑250 (MQ=255) gCGGTCATGATTAACTGATGAGGATACACGCCTGCCTGATTACCTTTTTCCCGCAGCATGAGTCGTTGGTGCACGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGc < 1:678743/250‑1 (MQ=255) ggTCATGATTAACTGATGAGGATACACGCCTGCCTGATTACCTTTTTCCCGCAGCATGAGTCGTTGGTGCACGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCaa > 1:239742/1‑251 (MQ=255) cATGATTAACTGATGAGGATACACGCCTGCCTGATTACCTTTTTCCCGCAGCATGAGTCGTTGGTGCACGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCg > 1:560901/1‑251 (MQ=255) tAACTGATGAGGATACACGCCTGCCTGATTACCTTTTTCCCGCAGCATGAGTCGTTGGTGCACGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCaa > 1:636419/1‑251 (MQ=255) gatgaGGATACACGCCTGCCTGATTACCTTTTTCCCGCAGCATGAGTCGTTGGTGCACGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAAcc > 1:113746/1‑249 (MQ=255) gatgaGGATACACGCCTGCCTGATTACCTTTTTCCCGCAGCATGAGTCGTTGGTGCACGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCaa > 1:351667/1‑251 (MQ=255) ggATACACGCCTGCCTGATTACCTTTTTCCCGCAGCATGAGTCGTTGGTGCACGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAAcggcg > 1:97913/1‑251 (MQ=255) aTACACGCCTGCCTGATTACCTTTTTCCCGCAGCATGAGTCGTTGGTGCACGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTc > 1:862695/1‑203 (MQ=255) tgcctgATTACCTTTTTCCCGCAGCATGAGTCGTTGGTGCACGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACgg < 1:653279/251‑1 (MQ=255) tgcctgATTACCTTTTTCCCGCAGCATGAGTCGTTGGTGCACGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACgg > 1:684413/1‑251 (MQ=255) tgcctgATTACCTTTTTCCCGCAGCATGAGTCGTTGGTGCACGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACgg < 1:772041/251‑1 (MQ=255) aTTACCTTTTTCCCGCAGCATGAGTCGTTGGTGCACGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGc < 1:327397/250‑1 (MQ=255) ccTTTTTCCCGCAGCATGAGTCGTTGGTGCACGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTc < 1:873725/251‑1 (MQ=255) ccTTTTTCCCGCAGCATGAGTCGTTGGTGCACGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTc < 1:342815/251‑1 (MQ=255) ccTTTTTCCCGCAGCATGAGTCGTTGGTGCACGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTc < 1:96066/251‑1 (MQ=255) ccTTTTTCCCGCAGCATGAGTCGTTGGTGCACGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTc < 1:531004/251‑1 (MQ=255) ccTTTTTCCCGCAGCATGAGTCGTTGGTGCACGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTc < 1:473411/251‑1 (MQ=255) ttttCCCGCAGCATGAGTCGTTGGTGCACGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCg > 1:43929/1‑250 (MQ=255) cGCAGCATGAGTCGTTGGTGCACGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTCCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGAt < 1:411969/251‑1 (MQ=255) cagcaTGAGTCGTTGGTGCACGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGAttt > 1:574917/1‑251 (MQ=255) gcaTGAGTCGTTGGTGCACGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTgccagcc > 1:725469/1‑180 (MQ=255) gcaTGAGTCGTTGGTGCACGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTc > 1:900907/1‑250 (MQ=255) gcaTGAGTCGTTGGTGCACGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTc > 1:762186/1‑250 (MQ=255) aTGAGTCGTTGGTGCACGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGt < 1:795299/251‑1 (MQ=255) aTGAGTCGTTGGTGCACGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGt < 1:413452/251‑1 (MQ=255) aTGAGTCGTTGGTGCACGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGt < 1:880440/251‑1 (MQ=255) tCGTTGGTGCACGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTCGgcg > 1:517311/1‑251 (MQ=255) tCGTTGGTGCACGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTCGgcg > 1:666353/1‑251 (MQ=255) gTTGGTGCACGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAAcc > 1:560512/1‑201 (MQ=255) cGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCGCCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTCGGCGCCATCAATGc > 1:670523/1‑250 (MQ=255) cGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTc < 1:134717/236‑1 (MQ=255) ccGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTg < 1:253019/170‑1 (MQ=255) gAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGtaaaattctaccgcacttacg > 1:657668/1‑231 (MQ=255) gTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAAcc > 1:718279/1‑182 (MQ=255) gTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTCGGCGCCATCAATGCCACCTGTTTa > 1:711513/1‑250 (MQ=255) gTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTCGGCGCCATCAATGCCACCTGTTTa > 1:830028/1‑250 (MQ=255) gTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTCGGCGCCATCAATGCCACCTGttt < 1:695147/244‑1 (MQ=255) cGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTCGGCGCCATCAATGCCACCTGTTTACCGTTATCa < 1:203698/251‑1 (MQ=255) acaaTCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTc < 1:828482/197‑1 (MQ=255) caaTCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTCGGCGCCATCAATGCCACCTGTTTACCGTTATCAAt < 1:642587/247‑1 (MQ=255) cACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTCGGCGCCATCAATGCCAcc > 1:505052/1‑226 (MQ=255) aGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTCGGCGCCATCAATGCCACCTGTTTACCGTTATCAATGGCGAGCAGTGc > 1:582439/1‑250 (MQ=255) aGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTCGGCGCCATCAATGCCACCTGTTTACCGTTATCAATGGCGAGCAGTGc > 1:39375/1‑250 (MQ=255) gCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAAcgg > 1:581024/1‑163 (MQ=255) cTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTCGGCGCCATCAATGCCACCTGTTTACCGTTATCAATGGCGAGCAGTGCCg < 1:293822/250‑1 (MQ=255) aaCGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTCGGCGCCATCAATGCCAcc < 1:234839/215‑1 (MQ=255) aTTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTCGGCGCCATCAATGcc > 1:756161/1‑203 (MQ=255) tCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCGGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTCGGCGCCATCAATGcc > 1:123189/1‑192 (MQ=255) tCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTCGGCGCCATCAATGCCACCTGTTTACCGTTATCAATGGCGAGCAGTGCCGCTAACGCCGCCACTAAGGTTTTAcc < 1:765686/251‑1 (MQ=255) ggAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGa < 1:436847/165‑1 (MQ=255) ggAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTCGGCGCCATCAATGCCACCTGttt < 1:251638/196‑1 (MQ=255) aaTAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTCGGCGCCATCAATGCCACCTGTTTACCGTTATCAATGGCGAGCAGTGCCGCTAACGCCGCCACTAAGGTTTTACCGGAGc < 1:738645/250‑1 (MQ=255) aCGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTCGGCGCCATCAATGCCACCTGTTTACCGTTATCAATGGCGAGCAGTGCCGCTAACGCCGCCACTAAGGTTTTACCGGAGCCGACa > 1:592031/1‑251 (MQ=255) gtTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAAcc > 1:251961/1‑115 (MQ=255) ttCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTCGGCGCCATCAATGCCACCTGTTTACCGTTATCAATGGCGAGCAGTGCCGCTAACGCCGCCACTAAGGTTTTACCGGAGCCGACATCCCCCTg > 1:48458/1‑250 (MQ=255) tCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTCGGCGCCATCAATACCACCTGTTTACCGTTATCAATGGCGAGCAGTGCCGCTAACGCCGCCACTAAGGTTTTACCGGAGCCGACATCCCCCTGCa > 1:432831/1‑251 (MQ=255) ccaccaTTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTCGGCGCCATCAATGCCACCTGTTTACCGTTATCAAt > 1:821683/1‑187 (MQ=255) caccaTTTGTCCCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTCGGCGCCATCAATGCCACCTGTTTACCGTTATCAATGGCGAGCAGTGCCGCTAACGCCGCCACTAAGGTTTTACCGGAGCCGACATCCCCCTGCACCAGAc < 1:254155/251‑1 (MQ=255) ccaTTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTCGGCGCCATCAATGCCACCTGTTTACCGt < 1:838575/177‑1 (MQ=255) ccaTTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTCGGCGCCATCAATGCCACCTGTTTACCGTTATCAATGGCGAGCAGTGCCGCTAACGCCGCCACTAAGGTTTTACCGGAGCCGACATCCCCCTGCACCAGACg > 1:391914/1‑250 (MQ=255) ccaTTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTCGGCGCCATCAATGCCACCTGTTTACCGTTATCAATGGCGAGCAGTGCCGCTAACGCCGCCACTAAGGTTTTACCGGAGCCGACATCCCCCTGCACCAGACg > 1:700574/1‑250 (MQ=255) caTTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTCGGCGCCATCAATGCCACCTGTTTACCGTTATCAATGGCGAGCAGTGCCGCTAATGCCGCCACTAAGGTTTTACCGGAGCCGACATCCCCCTGCACCAGACGca < 1:189907/251‑1 (MQ=255) tttGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTCGGCGCCATCAATGCCACCTGTTTACCGTTATCAATGGCGAGCAGTGCCGCTAACGCCGCCACTAAGGTTTTACCGGAGCCGACATCCCCCTGCACCAGACGcatc < 1:271886/251‑1 (MQ=255) gttGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTCGGCGCCATCAATGCCACCTGTTTACCGTTATCAATGGCGAGCAGTGCCGCTAACGCCGCCACTAAGGTTTTACCGGAGCCGACATcccc > 1:801585/2‑235 (MQ=255) ttGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTCGGCGCCATCAATGCCACCTGTTTACCGTTATCAATGGCGAGCAGTGCCGCTAACGCCGCCACTAAGGTTTTACCGGAGCCGACATccccc < 1:125521/235‑1 (MQ=255) ttGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTCGGCGCCATCAATGCCACCTGTTTACCGTTATCAATGGCGAGCAGTGCCGCTAACGCCGCCACTAAGGCTTTACCGGAGCCGACATCCCCCTGCACCAGACGcatca > 1:161973/1‑251 (MQ=255) gTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTCGGCGCCATCAATGCCACCTGTTTACCGTTATCAATGGCGAGCAGTGCCGCTAACGCCGCCACTAAGGTTTTACCGGAGCCGACATCCCCCTGCACCAGACGcatcat > 1:583835/1‑250 (MQ=255) gTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTCGGCGCCATCAATGCCACCTGTTTACCGTTATCAATGGCGAGCAGTGCCGCTAACGCCGCCACTAAGGTTTTACCGGAGCCGACATCCCCCTGCACCAGACGcatcat > 1:562099/1‑250 (MQ=255) tctcTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTCGGCGCCATCAATGCCACCTGTTTACCGTTATCAATGGCGAGCAGTGCCGCTAACGCCGCCACTAAGGTTTTACCGGAGCCGACATCCCCCTGCACCAGACGCATCATCGGGTAAGGt > 1:790370/1‑239 (MQ=255) tctcTAGCTCGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTCGGCGCCATCAATGCCACCTGTTTACCGTTATCAATGGCGAGCAGTGCCGCTAACGCCGCCACTAAGGTTTTACCGGAGCCGACATCCCCCTGCACCAGACGCATCATCGGGTAAGGTTGCGCCACATcc < 1:583699/251‑1 (MQ=255) ccGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTCGGCGCCATCAATGCCACCTGTTTACCGTTATCAATGGCGAGCAGTGCCGCTAACGCCGCCACTAAGGTTTTACCGGAGCCGACATCCCCCTGCACCAGACGCATCATCGGGTAAGGTTGCGCCACATCCCGTTCAat < 1:366837/250‑1 (MQ=255) cGGCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTCGGCGCCATCAATGCCACCTGTTTACCGTTATCAATGGCGAGCAGTGCCGCTAACGCCGCCACTAAGGTTTTACCGGAGCCGACATCCCCCTGCACCAGACGCATCATCGGGTAAGGt > 1:763328/1‑230 (MQ=255) gCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTCGGCGCCATCAATGCCACCTGTTTACCGTTATCAATGGCGAGCAGTGCCGCTAACGCCGCCACTAAGGTTTTACCGGAGCCGACATCCCCCTGCACCAGACGCATCATCGGGTAAGGTTGCGCCACATCCCGTTCAatat < 1:77196/250‑1 (MQ=255) gCAACCCGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTCGGCGCCATCAATGCCACCTGTTTACCGTTATCAATGGCGAGCAGTGCCGCTAACGCCGCCACTAAGGTTTTACCGGAGCCGACATCCCCCTGCACCAGACGCATCATCGGGTAAGGTTGCGCCACATCCCGTTCAatat < 1:148507/250‑1 (MQ=255) cccGTTGTTTGCCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTCGGCGCCATCAATGCCACCTGTTTACCGTTATCAATgg > 1:526185/1‑144 (MQ=255) | GTCATGGCAAGCGTACGTGGAATCGGTGTGGCGGTCATGATTAACTGATGAGGATACACGCCTGCCTGATTACCTTTTTCCCGCAGCATGAGTCGTTGGTGCACGCCGAAACGGTGCTGTTCGTCAACAATCACCAAGCTCAAACGATGAAATTCCACCTCTTCCTGGAATAACGCGTGCGTTCCCACCACCATTTGTACCGCACCGCTTTTTATTTTCTCTAGCTCGGCAACCCGTTGTTTGTCTTTGACTTTTCCTGCCAGCCAGCCCACTTGAATGCCAAGCGGTTCAAACCAACGGCGGAAGTTCACGGCGTGCTGTTCCGCTAAGATTTCCGTCGGCGCCATCAATGCCACCTGTTTACCGTTATCAATGGCGAGCAGTGCCGCTAACGCCGCCACTAAGGTTTTACCGGAGCCGACATCCCCCTGCACCAGACGCATCATCGGGTAAGGTTGCGCCACATCCCGTTCAATAT > 1/1209950‑1210427 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |