Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,208,826	T→C	T121T (ACA→ACG)	unknown ←	hypothetical protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,208,826	0	T	C	100.0%	149.7 / NA	56	T121T (ACA→ACG)	unknown	hypothetical protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (34/22);  total (34/22)

ACCTCATGATAAACACTAAAGCCGCCGACGCCGGAATCAAAAAAAAGAATTGTGGGTTTTGTTTGAATGTCCATAATCAGTTTGATGGAAAAATTTGCGTCATTTTATTCGCTTATAAAGCGGTTCTCAAGGAAAAGCTCGCTGATTAACATCGGTATGTTTTGGACCCAAAACAAGGAACGACGGGCATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGTGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCTGGGGAATGAGCGTTCGAGCCTCGATCCAAGGTTTGCCGTCACCAAGCAATAAAACTTCACGGCACCAATAGGCTCCCGCCGGCAAAAGTGCAGTCTCATTTTCAAATGTTTTTTCTATCCATTTTTCTGACCGCACTTTCACGTCAAATCGTGCACAAGTCTGT  >  1/1208587‑1209066                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                 aCCTCATGATAAACACTAAAGCCGCCGACGCCGGAATCAAAAAAAAGAATTGTGGGTTTTGCTTGAATGTCCATAATCAGTTTGATGGAAAAATTTGCGTCATTTTATTCGCTTATAAGGCGGTTCTCAAGGAAAAGCTCGCTGATTAACATCGGTATGTTTTGGACCCGAAACAAGGAACGACGGGCATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGaaa                                                                                                                                                                                                                                       >  1:425147/1‑251 (MQ=255)                                      cAAAAAAAAGAATTGTGGGTTTTGCTTGAATGTCCATAATCAGTTTGATGGAAAAATTTGCGTCATTTTATTCGCTTATAAGGCGGTTCTCAAGGAAAAGCTCGCTGATTAACATCGGTATGTTTTGGACCCAAAACAAGGAACGACGGGCATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGAc                                                                                                                                                                                                  <  1:717372/251‑1 (MQ=255)                                        aaaaaaaGAATTGTGGGTTTTGCTTGAATGTCCATAATCAGTTTGATGGAAAAATTTGCGTCATTTTATTCGCTTATAAGGCGGTTCTCAAGGAAAAGCTCGCTGATTAACATCGGTATGTTTTGGACCCAAAACAAGGAACGACGGGCATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAg                                                                                                                                                                                                <  1:42610/251‑1 (MQ=255)                                               gAATTGTGGGTTTTGCTTGAATGCCCATAATCAGTTTGATGGAAAAATTTGCGTCATTTTATTCGCTTATAAGGCGGTTCTCAAGGAAAAGCTCGCTGATTAACATCGGTATGTTTTGGACCCAAAACAAGGAACGACGGGCATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTgg                                                                                                                                                                                         >  1:665580/1‑251 (MQ=255)                                                             gcttgAATGTCCATAATCAGTTTGATGGAAAAATTTGCGTCATTTTATTCGCTTATAAGGCGGTTCTCAAGGAAAAGCTCGCTGATTAACATCGGTATGTTTTGGACCCAAAACAAGGAACGACGGGCATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGAc                                                                                                                                                                                 >  1:186476/3‑245 (MQ=255)                                                              cttgAATGTCCATAATCAGTTTGATGGAAAAATTTGCGTCATTTTATTCGCTTATAAGGCGGTTCTCAAGGAAAAGCTCGCTGATTAACATCGGTATGTTTTGGACCCAAAACAAGGAACGACGGGCATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAg                                                                                                                                                                           <  1:638268/249‑1 (MQ=255)                                                                     gTCCATAATCAGTTTGATGGAAAAATTTGCGTCATTTTATTCGCTTATAAGGTGGTTCTCAAGGAAAAGCTCGCTGATTAACATCGGTATGTTTTGGACCCAAAACAAGGAACGACGGGCATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAg                                                                                                                                                                                                <  1:426683/222‑1 (MQ=255)                                                                     gTCCATAATCAGTTTGATGGAAAAATTTGCGTCATTTTATTCGCTTATAAGGCGGTTCTCAAGGAAAAGCTCGCTGATTAACATCGGTATGTTTTGGACCCAAAACAAGGAACGACGGGCATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCTggg                                                                                                                                                                   >  1:516228/1‑251 (MQ=255)                                                                             tCAGTTTGATGGAAAAATTTGCGTCATTTTATTCGCTTATAAGGCGGTTCTCAAGGAAAAGCTCGCTGATTAACATCGGTATGTTTTGGACCCAAAACAAGGAACGACGGGCATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCTGGGGAATGAGc                                                                                                                                                           <  1:840179/251‑1 (MQ=255)                                                                                        gAAAAATTTGCGTCATTTTATTCGCTTATAAGGCGGTTCTCAAGGAAAAGCTCGCTGATTAACATCGGTATGTTTTGGACCCAAAACAAGGAACGACGGGCATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCTGGGGAATGAGCGTTCGAGCCt                                                                                                                                                 <  1:843796/250‑1 (MQ=255)                                                                                                  cGTCATTTTATTCGCTTATAAGGCGGTTCTCAAGGAAAAGCTCGCTGATTAACATCGGTATGTTTTGGACCCAAAACAAGGAACGACGGGCATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCTGGGGAATGAGCGTTCGAGCCTCGATCCAAgg                                                                                                                                       <  1:574927/250‑1 (MQ=255)                                                                                                  cGTCATTTTATTCGCTTATAAGGCGGTTCTCAAGGAAAAGCTCGCTGATTAACATCGGTATGTTTTGGACCCAAAACAAGGAACGACGGGCATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCTGGGGAATGAGCGTTCGAGCCTCGATCCAAGGt                                                                                                                                      <  1:217461/251‑1 (MQ=255)                                                                                                                cTTATAAGGCGGTTCTCAAGGAAAAGCTCGCTGATTAACATCGGTATGTTTTGGACCCAAAACAAGGAACGACGGGCATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGcc                                                                                                                                                                                                                                  >  1:134162/1‑145 (MQ=255)                                                                                                                cTTATAAGGCGGTTCTCAAGGAAAAGCTCGCTGATTAACATCGGTATGTTTTGGACCCAAAACAAGGAACGACGGGCATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGcc                                                                                                                                                                                                                                  >  1:712834/1‑145 (MQ=255)                                                                                                                cTTATAAGGCGGTTCTCAAGGAAAAGCTCGCTGATTAACATCGGTATGTTTTGGACCCAAAACAAGGAACGACGGGCATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAg                                                                                                                                                                                                <  1:888284/179‑1 (MQ=255)                                                                                                                cTTATAAGGCGGTTCTCAAGGAAAAGCTCGCTGATTAACATCGGTATGTTTTGGACCCAAAACAAGGAACGACGGGCATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAg                                                                                                                                                                           >  1:808033/1‑200 (MQ=255)                                                                                                                cTTATAAGGCGGTTCTCAAGGAAAAGCTCGCTGATTAACATCGGTATGTTTTGGACCCAAAACAAGGAACGACGGGCATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCg                                                                                                                                                                         >  1:308785/1‑202 (MQ=255)                                                                                                                cTTATAAGGCGGTTCTCAAGGAAAAGCTCGCTGATTAACATCGGTATGTTTTGGACCCAAAACAAGGAACGACGGGCATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCTGGGGAATGAGCGTTCGAGCCTCGATCCAAGGTTTGCCGTCACCAAg                                                                                                                        >  1:846635/1‑251 (MQ=255)                                                                                                                cTTATAAGGCGGTTCTCAAGGAAAAGCTCGCTGATTAACATCGGTATGTTTTGGACCCAAAACAAGGAACGACGGGCATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCTGGGGAATGAGCGTTCGAGCCTCGATCCAAGGTTTGCCGTCACCAAg                                                                                                                        >  1:684280/1‑251 (MQ=255)                                                                                                                      aggCGGTTCTCAAGGAAAAGCTCGCTGATTAACATCGGTATGTTTTGGACCCAAAACAAGGAACGACGGGCATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCTGGGGAATGAGc                                                                                                                                                           >  1:471968/3‑210 (MQ=255)                                                                                                                           gTTCTCAAGGAAAAGCTCGCTGATTAACATCGGTATGTTTTGGACCCAAAACAAGGAACGACGGGCATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCTGGGGAATGAGCGTTCGAGCCt                                                                                                                                                 >  1:415682/1‑215 (MQ=255)                                                                                                                            ttCTCAAGGAAAAGCTCGCTGATTAACATCGGTATGTTTTGGACCCAAAACAAGGAACGACGGGCATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCTGGGGAATGAGCGTTCGAGCCTCGATCCAAGGTTTGCCGTCACCAAGCAATAAAACtt                                                                                                             <  1:616248/250‑1 (MQ=255)                                                                                                                                   ggAAAAGCTCGCTGATTAACATCGGTATGTTTTGGACCCAAAACAAGGAACGACGGGCATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCTGGGGAATGAGCGTTCGAGCCTCGATCCAAGGTTTGCCGTCACCAAGCAATAAAACTTCACGGc                                                                                                       >  1:81210/1‑249 (MQ=255)                                                                                                                                          cTCGCTGATTAACATCGGTATGTTTTGGACCCAAAACAAGGAACGACGGGCATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCTGGGGAATGAGCGTTCGAGCCTCGATCCAAGGTTTGCCGTCACCAAGCAATAAAACTTCACGGCACCAATa                                                                                                <  1:873149/249‑1 (MQ=255)                                                                                                                                          cTCGCTGATTAACATCGGTATGTTTTGGACCCAAAACAAGGAACGACGGGCATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCTGGGGAATGAGCGTTCGAGCCTCGATCCAAGGTTTGCCGTCACCAAGCAATAAAACTTCACGGCACCAATAgg                                                                                              <  1:389258/251‑1 (MQ=255)                                                                                                                                           tCGCTGATTAACATCGGTATGTTTTGGACCCAAAACAAGGAACGACGGGCATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCTGGGGAATGAGCGTTCGAGCCTCGATCCAAGGTTTGCCGTCACCAAGCAATAAAACTTCACGGCACCAATAGGc                                                                                             <  1:18270/251‑1 (MQ=255)                                                                                                                                            cGCTGATTAACATCGGTATGTTTTGGACCCAAAACAAGGAACGACGGGCATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCTGGGGAATGAGCGTTCGAGCCTCGATCCAAGGTTTGCCGTCACCAAGCAATAAAACTTCACGGCACCAATAGGCt                                                                                            <  1:501812/251‑1 (MQ=255)                                                                                                                                                gATTAACATCGGTATGTTTTGGACCCAAAACAAGGAACGACGGGCATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCTGGGGAATGAGCGTTCGAGCCTCGATCCAAGGTTTGCCGTCACCAAGCAATAAAAc                                                                                                               <  1:392769/228‑1 (MQ=255)                                                                                                                                                gATTAACATCGGTATGTTTTGGACCCAAAACAAGGAACGACGGGCATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCTGGGGAATGAGCGTTCGAGCCTCGATCCAAGGTTTGCCGTCACCAAGCAATAAAACTTCACGGCACCAATAGGCTCccg                                                                                        >  1:683486/1‑251 (MQ=255)                                                                                                                                                      cATCGGTATGTTTTGGACCCAAAACAAGGAACGACGGGCATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCTGGGGAATGAGCGTTCGAGCCTCGATCCAAGGTTTGCCGTCACCAAGCAATAAAACTTCACGGCACCAATAGGCTcc                                                                                          >  1:16798/1‑243 (MQ=255)                                                                                                                                                        tCGGTATGTTTTGGACCCAAAACAAGGAACGACGGGCATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCTGGGGAATGAGCGTTCGAGCCTCGATCCAAGGTTTGCCGTCACCAAGCAATAAAACTTCACGGCACCAATAGGCTCCCGCCGGCaaa                                                                                >  1:381153/1‑251 (MQ=255)                                                                                                                                                           gTATGTTTTGGACCCAAAACAAGGAACGACGGGCATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCTGGGGAATGAGCGTTCGAGCCTCGATCCAAGGTTTGCCGTCACCAAGCAATAAAACTTCACGGCACCAATAGGCTCCCGCCGGCAAAAGt                                                                             >  1:393101/1‑251 (MQ=255)                                                                                                                                                               gTTTTGGACCCAAAACAAGGAACGACGGGCATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAg                                                                                                                                                                           <  1:171284/153‑1 (MQ=255)                                                                                                                                                                  ttGGACCCAAAACAAGGAACGACGGGCATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCTTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCTGGGGAATGAGCGTTCGAGCCTCGATCCAAGGTTTGCCGTCACCAAGCAATAAAACTTCACGGCACCAATAGGCTCCCGCCGGCAAAAGTGCAGtct                                                                      <  1:892185/251‑1 (MQ=255)                                                                                                                                                                      aCCCAAAACAAGGAACGACGGGCATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCTGGGGAATGAGCGTTCGAGCCTCGATCCAAGGTTTGCCGTCACCAAGCAATAAAACTTCACGGCACCAATAGGCTCCCGCCGGCAAAAGTGCAGTCTCAtt                                                                  >  1:159590/1‑251 (MQ=255)                                                                                                                                                                           aaaCAAGGAACGACGGGCATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCTGGGGAATGAGCGTTCGAGCCTCGATCCAAGGTTTGCCGTCACCAAGCAATAAAACTTCACGGCACCAATAGGCTCCCGCCGGCAAAAGTGCAGTCTCATTTTCaa                                                             >  1:140982/1‑251 (MQ=255)                                                                                                                                                                             aCAAGGAACGACGGGCATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCTGGGGAATGAGCGTTCGAGCCTCGATCCAAGGTTTGCCGTCACCAAGCAATAAAACTTCACGGCACCAATAGGCTCCCGCCGGCAAAAGTGCAGTCTCATTTTCAAAt                                                           >  1:226941/1‑251 (MQ=255)                                                                                                                                                                                          ggCATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCTGGGGAATGAGCGTTCGAGCCTCGATCCAAGGTTTGCCGTCACCAAGCAATAAAACTTCATGGCACCAATAGGCTCCCGCCGGCAAAAGTGCAGTCTCATCTTCAAATg                                                          >  1:500812/1‑239 (MQ=255)                                                                                                                                                                                          ggCATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCTGGGGAATGAGCGTTCGAGCCTCGATCCAAGGTTTGCCGTCACCAAGCAATAAAACTTCACGGCACCAATAGGCTCCCGCCGGCAAAAGTGCAGTCTCATTTTCAAATGTTTTTTCTATc                                               >  1:389176/1‑250 (MQ=255)                                                                                                                                                                                           gCATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCTGGGGAATGAGCGTTCGAGCCTCGATCCAAGGTTTGCCGTCACCAAGCAATAAAACTTCACGGCACCAATAGGCTCCCGCCGGCAAAAGTGCAGTCTCATTTTCAAATGTTTTTTCTATCCa                                             >  1:868230/1‑251 (MQ=255)                                                                                                                                                                                            cATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCTGGGGAATGAGCGTTCGAGCCTCGATCCAAGGTTTGCCGTCACCAAGCAATAAAACTTCACGGCACCAATAGGCTCCCGCCGGCAAAAGTGCAGTCTCATTTTCAAATGTTTTTTc                                                   >  1:110829/1‑244 (MQ=255)                                                                                                                                                                                            cATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCTGGGGAATGAGCGTTCGAGCCTCGATCCAAGGTTTGCCGTCACCAAGCAATAAAACTTCACGGCACCAATAGGCTCCCGCCGGCAAAAGTGCAGTCTCATTTTCAAATGTTTTTTCTATCCAt                                            >  1:562779/1‑251 (MQ=255)                                                                                                                                                                                                 aaTCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCTGGGGAATGAGCGTTCGAGCCTCGATCCAAGGTTTGCCGTCACCAAGCAATAAAACTTCACGGCACCAATAGGCTCCCGCCGGCAAAAGTGCAGtct                                                                      >  1:131748/1‑220 (MQ=255)                                                                                                                                                                                                 aaTCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCTGGGGAATGAGCGTTCGAGCCTCGATCCAAGGTTTGCCGTCACCAAGCAATAAAACTTCACGGCACCAATAGGCTCCCGCCGGCAAAAGTGCAGTCTCATTTTCAAATGTTTTTTCTATCCAttttt                                        >  1:858811/1‑250 (MQ=255)                                                                                                                                                                                                               aTCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTgg                                                                                                                                                                                         >  1:555914/1‑91 (MQ=255)                                                                                                                                                                                                               aTCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCTGGGGAATGAGCGTTCGAGCCTCGATCCAAGGTTTGCCGTCACCAAGCAATAAAACTTCACGGCACCAATAGGCTCCCGCCGGCAAAAGTGCAGTCTCATTTTCAAATGTTTTTTCTATCCATTTTTCTGACCGCACTTTCa                         >  1:96890/1‑251 (MQ=255)                                                                                                                                                                                                               aTCTTCCGCCAATTGGTTTACCCGCTTTTGGGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCTGGGGAATGAGCGTTCGAGCCTCGATCCAAGGTTTGCCGTCACCAAGCAATAAAACTTCACGGCACCAATAGGCTCCCGCCGGCAAAAGTGCAGTCTCATTTTCAAATGTTTTTTCTATCCATTTTTCTGACCGCACTTTCa                         <  1:255162/251‑1 (MQ=255)                                                                                                                                                                                                                tCTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCTGGGGAATGAGCGTTCGAGCCTCGATCCAAGGTTTGCCGTCACCAAGCAATAAAACTTCACGGCACCAATAGGCTCCCGCCGGCAAAAGTGCAGTCTCATTTTCAAATGTTTTTTCTATCCATTTTTCTGACCGCACTTTCa                         >  1:686534/1‑250 (MQ=255)                                                                                                                                                                                                                 cTTGCGCCCATTGGATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCTGGGGAATGAGCGTTCGAGCCTCGATCCAAGGTTTGCCGTCACCAAGCAATAAAACTTCACGGCACCAATAGGCTCCCGCCGGCAAAAGTGCAGTCTCATTTTCAAATGTTTTTTCTATCCATTTTTCTGACCGCACTTTCACg                       >  1:712039/1‑251 (MQ=255)                                                                                                                                                                                                                             ggATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCTGGGGAATGAGCGTTCGAGCCTCGATCCAAGGTTTGCCGTCAc                                                                                                                            <  1:316079/138‑1 (MQ=255)                                                                                                                                                                                                                             ggATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCTGGGGAATGAGCGTTCGAGCCTCGATCCAAGGTTTGCCGTCACCAAGCAATAAAACTTCACGGCACCAATAGGCTCCCGCCGGCAAAAGTGCAGTCTCATTTTCAAATGTTTTTTCTATCCATTTTTCTGACCGCACt                             >  1:409170/1‑233 (MQ=255)                                                                                                                                                                                                                             ggATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCTGGGGAATGAGCGTTCGAGCCTCGATCCAAGGTTTGCCGTCACCAAGCAATAAAACTTCACGGCACCAATAGGCTCCCGCCGGCAAAAGTGCAGTCTCATTTTCAAATGTTTTTTCTATCCATTTTTCTGACCGCACTTTCACGTCAAATCGTGca           >  1:719012/1‑251 (MQ=255)                                                                                                                                                                                                                             ggATTAACCGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCTGGGGAATGAGCGTTCGAGCCTCGATCCAAGGTTTGCCGTCACCAAGCAATAAAACTTCACGGCACCAATAGGCTCCCGCCGGCAAAAGTGCAGTCTCATTTTCAAATGTTTTTTCTATCCATTTTTCTGACCGCACTTTCACGTCAAATCGTGca           >  1:653698/1‑251 (MQ=255)                                                                                                                                                                                                                               tttacccGCCTTTGTGGCGTTTGCTGAAATAGCCATTCGCCGATTGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCTGGGGAATGGGCGTTCGAGCCTCGATCCAAGGTTTGCCGTCACCAAGCAATAAAACTTCACGGCACAAATAGGCTCCCGCCGGCAAAAGTGCAGTCTCATTTTCAAATGTTTTTTCTATCCATTTTTCTGACCGCACTTTCACGTCAAATCGTGcac          <  1:507839/245‑1 (MQ=255)                                                                                                                                                                                                                               aTTAACCGCTTTTGTGGCGTTTGCTAAAATAGCCATTCGCCGATAGTTTTTTTGCCTAACATTGACAGTTCTTGTTGATAGACAAATAGCGTCTGGGGAATGGGCGTTCGAGCCTCGATCCAAGGTTTGCCGTCACCAAGCAATAAAACTTCACGGCACCAATAGGCTCCCGCCGGCAAAAGTGCAGTCTCATTTTCAAATGTTTTTTCTATCCATTTTTCTGACCGCACTTTCACGTCAAATCGTGcac          <  1:314706/250‑1 (MQ=255)                                                                                                                                                                                                                                       cttttGTGGCGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCGGGGGAATGAGCGTTCGAGCCTCGATCCAAGGTTTGCCGTCACCAAGCAATAAAACTTCACGGCACCAATAGGCTCCCGCCGGCAAAAGTGCAGTCTCATTTTCAAATGTTTTTTCTATCCATTTTTCTGACCGCACTTTCACGTCAAATCGTGCACAAGTCTGt  <  1:412881/248‑1 (MQ=255)                                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                 ACCTCATGATAAACACTAAAGCCGCCGACGCCGGAATCAAAAAAAAGAATTGTGGGTTTTGTTTGAATGTCCATAATCAGTTTGATGGAAAAATTTGCGTCATTTTATTCGCTTATAAAGCGGTTCTCAAGGAAAAGCTCGCTGATTAACATCGGTATGTTTTGGACCCAAAACAAGGAACGACGGGCATATAATCCGCTTTGAGGATCTTGCGCCCATTGGATTAACCGCCTTTGTGGTGTTTGCTGAAATAGCCATTCGCCGATAGGTTTTTTGCCTAACATTGACAGTTCTTGGTGATAGACAAATAGCGTCTGGGGAATGAGCGTTCGAGCCTCGATCCAAGGTTTGCCGTCACCAAGCAATAAAACTTCACGGCACCAATAGGCTCCCGCCGGCAAAAGTGCAGTCTCATTTTCAAATGTTTTTTCTATCCATTTTTCTGACCGCACTTTCACGTCAAATCGTGCACAAGTCTGT  >  1/1208587‑1209066

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑