Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,202,139	T→G	intergenic (+130/+465)	unknown → / ← unknown	Na+/H+ antiporter/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,202,139	0	T	G	100.0%	21.2 / NA	11	intergenic (+130/+465)	unknown/unknown	Na+/H+ antiporter/LSU rRNA ## 23S rRNA, large subunit ribosomal RNA
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (8/3);  total (8/3)

CCTTCAAAGGGCATATGAAAACCAATAGCGTACGGTTTTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGTTCGGTATGGTTTCAGGTGGTACCACCGCACTATCGCCGCCAATATTCTTTGATGACTATCCTCTCTCCTTTCCGCGCTTTCGCTTTCTTATTC  >  1/1202102‑1202372                                      |                                                                                                                                                                                                                                          cttaagctctttgattccatacgcaTAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGTTCGGTATGGTTTCAGGTGGTACCACCGCACTATCGCCGCCAATATTCTTTGATGACTATCCTCTCTCCTTTc                       >  1:519975/25‑250 (MQ=14) cttaagctctttgattccatacgcaTAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGCGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGTTCGGTATGGTTTCAGGTGGTACCACCGCACTATCGCCGCCAATATTCTTTGATGACTATCCTCTCTCCTTTc                       >  1:5275/25‑250 (MQ=14)         ctttgattccatacgcaTAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGGCCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCAGAGTTCGGTATGGTTTCAGGTGGTACCACCGCACTATCGCCGCCAatat                                                  <  1:636028/199‑1 (MQ=14)         ctttgattccatacgcaTAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGTTCGGTATGGTTTCAGGAGGTACCACCGCCCTATCACCGCCAATCTTCTTTGATGACTATACTCTCTCCTTTCCGcgcttt               >  1:579189/17‑250 (MQ=14)           ttgattccatacgcaTAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGTTCGGTATGGTTTCAGGTGGTACCACCGCACTAACGCCGCCAATATTCTTTGATGACTATCCTCTCTCCTTTCCGcgctttcg             >  1:773199/15‑250 (MQ=255)                  catacgcaTAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGc                                                                                                                      >  1:707938/8‑138 (MQ=255)                     acgcaTAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGTTCGGTATGGTTTCAGGTGGTACCACCGCACTATCGCCGCCAATATTCTTTGATGACTATCCTCTCTCCTTTCCGCGCTTTCGCTTTCTTATTc  <  1:349581/247‑1 (MQ=255)                     acgcaTAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGTTCGGTATGGTTTCAGGTGGTACCACCGCACTATCGCCGCCAATATTCTTTGATGACTATCCTCTCTCCTTTCCGCGCTTTCGCTTTCTTATTc  <  1:41092/247‑1 (MQ=255)                      cgcaTAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGc                                                                                                              >  1:173328/4‑142 (MQ=255)                      cgcaTAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGTTCGGTATGGTTTCAGGTGGTACCACCGCACTATCGCCGCCAATATTCTTTGATGACTATCCTCTCTCCTTTCCGCGCTTTCGCTTTCTTATTc  >  1:19711/4‑250 (MQ=255)                      cgcaTAGCGTACGGTTGTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGTTCGGTATGGTTTCAGGTGGTACCACCGCACTATCGCCGCCAATATTCTTTGATGACTATCCTCTCTCCTTTCCGCGCTTTCGCTTTCTTATTc  >  1:495300/4‑250 (MQ=255)                                      |                                                                                                                                                                                                                                          CCTTCAAAGGGCATATGAAAACCAATAGCGTACGGTTTTTATAGCTAGACAACGTCTAGCTATAACAAAAAGCCCCGATGCTCTCACATCAGGGCTCCCTATCAGGCTTGGCGGTGACCTACTCTCACATGGGGATGCCCCACACTACCATCGGCATTACGGCCTTTCACTTCTGAGTTCGGTATGGTTTCAGGTGGTACCACCGCACTATCGCCGCCAATATTCTTTGATGACTATCCTCTCTCCTTTCCGCGCTTTCGCTTTCTTATTC  >  1/1202102‑1202372

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑