Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,201,351 A→G D292G (GAT→GGT)  unknown → Na+/H+ antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,201,3510AG98.6% 198.7 / ‑5.0 71D292G (GAT→GGT) unknownNa+/H+ antiporter
Reads supporting (aligned to +/- strand):  ref base A (0/1);  new base G (29/41);  total (29/42)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01

ATTTTCTCTATTTTAATGGTGTTCTTCGTAGCCTATTTCAGCTTTGACATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGAAACAGAAAATACGCAAGGAGAAGCGCTTTCAGGCATAAACGGTCATGTACGCAATTTAGTATTACCAATTATCACCCTTATTATCATAACAATTTCTGCCATGCTATACACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGATGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGCCTGGGTACTGGGCGTGAAATCCATGCTTGGCGCCATTTTAATTCTGTTTTTTGCCTGGACAATCAATAACGTGGTCAGCGATATAAAAACCGGCACTTATTTAGCCTCCTTGGTATCCTACGCC  >  1/1201107‑1201595
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    
aTTTTCTCTATTTTAATGGTGTTCTTCGTAGCCTATTTCAGCTTTGACATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGAAACAGAAAATACGCAAGGAGAAGCGCTTTCAGGCATAAACGGTCATGTACGCAATTTAGTATTACCAATTATCACCCTTATTATCATAACAATTTCTGCCATGCTATACACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCtt                                                                                                                                                                                                                                                >  1:4654/1‑251 (MQ=255)
    tctcTATTTTAATGGTGTTCTTCGTAGCCTATTTCAGCTTTGACATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGAAACAGAAAATACGCAAGGAGAAGCGCTTTCAGGCATAAACGGTCATGTACGCAATTTAGTATTACCAATTATCACCCTTATTATCATAACAATTTCTGCCATGCTATACACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGaa                                                                                                                                                                                                                                            >  1:105240/1‑251 (MQ=255)
            ttAATGGTGTTCTTCGTAGCCTATTTCAGCTTTGACATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGAAACAGAAAATACGCAAGGAGAAGCGCTTTCAGGCATAAACGGTCATGTACGCAATTTAGTATTACCAATTATCACCCTTATTATCATAACAATTTCTGCCATGCTATACACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATaccac                                                                                                                                                                                                                                    <  1:223647/251‑1 (MQ=255)
                                         cTTTGACATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGAAACAGAAAATACGCAAGGAGAAGCGCTTTCAGGCATAAACGGTCATGTACGCAATTTAGTATTACCAATTATCACCCTTATTATCATAACAATTTCTGCCATGCTATACACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGgtggtg                                                                                                                                                                                                       <  1:813894/251‑1 (MQ=255)
                                             gACATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGAAACAGAAAATACGCAAGGAGAAGCGCTTTCAGGCATAAACGGTCATGTACGCAATTTAGTATTACCAATTATCACCCTTATTATCATAACAATTTCTGCCATGCTATACACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCtt                                                                                                                                                                                                                      >  1:465056/1‑232 (MQ=255)
                                               cATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGAAACAGAAAATACGCAAGGAGAAGCGCTTTCAGGCATAAACGGTCATGTACGCAATTTAGTATTACCAATTATCACCCTTATTATCATAACAATTTCTGCCATGCTATACACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTg                                                                                                                                                                                                 <  1:114156/251‑1 (MQ=255)
                                               cATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGAAACAGAAAATACGCAAGGAGAAGCGCTTTCAGGCATAAACGGTCATGTACGCAATTTAGTATTACCAATTATCACCCTTATTATCATAACAATTTCTGCCATGCTATACACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTg                                                                                                                                                                                                 <  1:54306/251‑1 (MQ=255)
                                               cATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGAAACAGAAAATACGCAAGGAGAAGCGCTTTCAGGCATAAACGGTCATGTACGCAATTTAGTATTACCAATTATCACCCTTATTATCATAACAATTTCTGCCATGCTATACACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTg                                                                                                                                                                                                 <  1:825992/251‑1 (MQ=255)
                                               cATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGAAACAGAAAATACGCAAGGAGAAGCGCTTTCAGGCATAAACGGTCATGTACGCAATTTAGTATTACCAATTATCACCCTTATTATCATAACAATTTCTGCCATGCTATACACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTg                                                                                                                                                                                                 <  1:318098/251‑1 (MQ=255)
                                                     tCCCATGGCAAGACATGAAAGGCTGGCATTGGAAACAGAAAATACGCAAGGAGAAGCGCTTTCAGGCATAAACGGTCATGTACGCAATTTAGTATTACCAATTATCACCCTTATTATCATAACAATTTCTGCCATGCTATACACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGa                                                                                                                                                                                           <  1:302553/251‑1 (MQ=255)
                                                            gCAAGACATGAAAGGCTGGCATTGGAAACAGAAAATACGCAAGGAGAAGCGCTTTCAGGCATAAACGGTCATGTACGCAATTTAGTATTACCAATTATCACCCTTATTATCATAACAATTTCTGCCATGCTATACACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTg                                                                                                                                                                                     <  1:415129/250‑1 (MQ=255)
                                                             cAAGACATGAAAGGCTGGCATTGGAAACAGAAAATACGCAAGGAGAAGCGCTTTCAGGCATAAACGGTCATGTACGCAATTTAGTATTACCAATTATCACCCTTATTATCATAACAATTTCTGCCATGCTATACACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGc                                                                                                                                                                                    <  1:710310/250‑1 (MQ=255)
                                                                              gCATTGGAAACAGAAAATACGCAAGGAGAAGCGCTTTCAGGCATAAACGGTCATGTACGCAATTTAGTATTACCAATTATCACCCTTATTATCATAACAATTTCTGCCATGCTATACACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTAt                                                                                                                                                                   >  1:586911/1‑250 (MQ=255)
                                                                               cATTGGAAACAGAAAATACGCAAGGAGAAGCGCTTTCAGGCATAAACGGTCATGTACGCAATTTAGTATTACCAATTATCACCCTTATTATCATAACAATTTCTGCCATGCTATACACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATc                                                                                                                                                                  <  1:3268/250‑1 (MQ=255)
                                                                                        cAGAAAATACGCAAGGAGAAGCGCTTTCAGGCATAAACGGTCATGTACGCAATTTAGTATTACCAATTATCACCCTTATTATCATAACAATTTCTGCCATGCTATACACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGc                                                                                                                                                                                    >  1:549441/1‑223 (MQ=255)
                                                                                                       gagaAGCGCTTTCAGGCATAAACGGTCATGTACGCAATTTAGTATTACCAATTATCACCCTTATTATCATAACAATTTCTGCCATGCTATACACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATg                                                                                                                                           <  1:547646/249‑1 (MQ=255)
                                                                                                          aaGCGCTTTCAGGCATAAACGGTCATGTACGCAATTTAGTATTACCAATTATCACCCTTATTATCATAACAATTTCTGCCATGCTATACACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAatat                                                                                                                                      <  1:10500/251‑1 (MQ=255)
                                                                                                          aaGCGCTTTCAGGCATAAACGGTCATGTACGCAATTTAGTATTACCAATTATCACCCTTATTATCATAACAATTTCTGCCATGCTATACACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAatat                                                                                                                                      <  1:507931/251‑1 (MQ=255)
                                                                                                                          aaaCGGTCATGTACGCAATTTAGTATTACCAATTATCACCCTTATTATCATAACAATTTCTGCCATGCTATACACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGCCTGGGTAc                                                                                                                      <  1:873906/251‑1 (MQ=255)
                                                                                                                                 cATGTACGCAATTTAGTATTACCAATTATCACCCTTATTATCATAACAATTTCTGCCATGCTATACACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGCCTGGGTACTGGGCGt                                                                                                               >  1:420230/1‑251 (MQ=255)
                                                                                                                                      aCGCAATTTAGTATTACCAATTATCACCCTTATTATCATAACAATTTCTGCCATGCTATACACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGcc                                                                                                                             >  1:614397/1‑232 (MQ=255)
                                                                                                                                           aTTTAGTATTACCAATTATCACCCTTATTATCATAACAATTTCTGCCATGCTATACACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGCCTGGGTAc                                                                                                                      >  1:179774/1‑234 (MQ=255)
                                                                                                                                           aTTTAGTATTACCAATTATCACCCTTATTATCATAACAATTTCTGCCATGCTATACACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGCTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGCCTGGGTACTGGGCGTGAAATCCATg                                                                                                     >  1:678510/1‑251 (MQ=255)
                                                                                                                                                        aaTTATCACCCTTATTATCATAACAATTTCTGCCATGCTATACACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAg                                                                                                                                                                                                                   <  1:346663/128‑1 (MQ=255)
                                                                                                                                                              cACCCTTATTATCATAACAATTTCTGCCATGCTATACACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGCCTGGGTACTGGGCGTGAAATCCATGCTTGGCGCCATTTTAATTc                                                                                  <  1:219367/251‑1 (MQ=255)
                                                                                                                                                                cccTTATTATCATAGCAATTTCTGCCATGCTATACACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTAt                                                                                                                                                                        <  1:328120/163‑1 (MQ=255)
                                                                                                                                                                cccTTATTATCATAACAATTTCTGCCATGCTATACACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAata                                                                                                                                       >  1:212819/1‑196 (MQ=255)
                                                                                                                                                                            tAACAATTTCTGCCATGCTATACACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGCCTGGGTACTGGGCGTGAAATCCATGCTTGGCGCCATTTTAATTCTGTTTTTTGCCTgg                                                                    >  1:835515/1‑251 (MQ=255)
                                                                                                                                                                               cAATTTCTGCCATGCTATACACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGcc                                                                                                                             >  1:354032/1‑191 (MQ=255)
                                                                                                                                                                                   ttCTGCCATGCTATACACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGCCTGGGTACTGGGCGTGAAATCCATGCTTGGCGCCATTTTAATTCTGTTTTTTGCCTGGAcaatca                                                             <  1:109973/251‑1 (MQ=255)
                                                                                                                                                                                          aTGCTATACACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGgtg                                                                                                                                                                                                                            >  1:694606/1‑85 (MQ=255)
                                                                                                                                                                                            gCTATACACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGt                                                                                                                                                                                                  >  1:845708/1‑109 (MQ=255)
                                                                                                                                                                                            gCTATACACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCg                                                                                                                                                                                   >  1:567866/1‑124 (MQ=255)
                                                                                                                                                                                            gCTATACACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGcc                                                                                                                                                 <  1:236404/158‑1 (MQ=255)
                                                                                                                                                                                            gCTATACACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAata                                                                                                                                       <  1:157657/168‑1 (MQ=255)
                                                                                                                                                                                            gCTATACACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGCCTGGGTACTGGGc                                                                                                                 <  1:271484/190‑1 (MQ=255)
                                                                                                                                                                                            gCTATACACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGCCTGGGTACTGGGCGTGAAATCCATGCTTGGCGCCATTTTAATTCTGTTTTTTGCCTGGACAATCAATAACg                                                       <  1:645246/248‑1 (MQ=255)
                                                                                                                                                                                            gCTATACACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGCCTGGGTACTGGGCGTGAAATCCATGCTTGGCGCCATTTTAATTCTGTTTTTTGCCTGGACAATCAATAACGTgg                                                    >  1:690677/1‑251 (MQ=255)
                                                                                                                                                                                            gCTATACACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGCCTGGGTACTGGGCGTGAAATCCATGCTTGGCGCCATTTTAATTCTGTTTTTTGCCTGGACAATCAATAACGTgg                                                    >  1:690157/1‑251 (MQ=255)
                                                                                                                                                                                            gCTATACACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGCCTGGGTACTGGGCGTGAAATCCATGCTTGGCGCCATTTTAATTCTGTTTTTTGCCTGGACAATCAATAACGTgg                                                    >  1:757321/1‑251 (MQ=255)
                                                                                                                                                                                              tataCACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGCCTGGGTACTGGGCGTGAAATCCATGCTTGGCGCCATTTTAATTCTGTTTTTTGCCTGGACAATCAATAACGTGGTc                                                  <  1:389805/251‑1 (MQ=255)
                                                                                                                                                                                               ataCACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTg                                                                                                                                                                                     <  1:404578/119‑1 (MQ=255)
                                                                                                                                                                                               ataCACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGCCTGGGTACTGGGCGTGAAATCCATGCTTGGCGCCATTTTAATTCTGTTTTTTGCCTGGACAATCAATAACGTGGTCa                                                 >  1:67918/1‑251 (MQ=255)
                                                                                                                                                                                                  cacTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGc                                                                                                                                                                                                                                                   >  1:621899/1‑54 (MQ=255)
                                                                                                                                                                                                   acTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGCCTGGGTACTGGGCGTGAAATCCATGCTTGGCGCCATTTTAATTCTGTTTTTTGCCTGGACAATCAATAACGTGGTCAGCGa                                             <  1:855867/251‑1 (MQ=255)
                                                                                                                                                                                                    cTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGCCTGGGTACTg                                                                                                                    <  1:256023/179‑1 (MQ=255)
                                                                                                                                                                                                    cTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGCCTGGGTACTGGGCGTGAAATCCATGCTTGGCGCCATTTTAATTc                                                                                  <  1:172565/213‑1 (MQ=255)
                                                                                                                                                                                                    cTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGCCTGGGTACTGGGCGTGAAATCCATGCTTGGCGCCATTTTAATTCTGTTTTTTGCCTGGACAATCAATAACGTGGTCAGCGat                                            >  1:433543/1‑251 (MQ=255)
                                                                                                                                                                                                    cTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGCCTGGGTACTGGGCGTGAAATCCATGCTTGGCGCCATTTTAATTCTGTTTTTTGCCTGGACAATCAATAACGTGGTCAGCGat                                            >  1:38005/1‑251 (MQ=255)
                                                                                                                                                                                                         aaCCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGCCTGGGt                                                                                                                        <  1:454541/170‑1 (MQ=255)
                                                                                                                                                                                                         aaCCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGCCTGGGTACTGGGCGTGAAATCCATGCTTGGCGCCATTTTAATTCTGTTTTTTGCCTGGACAATCAATAACGTGGTCAGCGATATaaa                                       >  1:561805/1‑251 (MQ=255)
                                                                                                                                                                                                              aGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGcc                                                                                                                             <  1:815691/160‑1 (MQ=255)
                                                                                                                                                                                                              aGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGCCTGGGTACTGGGCGTGAAATCCATGCTTGGCGCCATTTTAATTCTGTTTTTTGCCTGGACAATCAATAACg                                                       <  1:216317/230‑1 (MQ=255)
                                                                                                                                                                                                              aGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGCCTGGGTACTGGGCGTGAAATCCATGCTTGGCGCCATTTTAATTCTGTTTTTTGCCTGGACAATCAATAACg                                                       <  1:523845/230‑1 (MQ=255)
                                                                                                                                                                                                                ccTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGCCTGGGTACTGGGCGTGAAATc                                                                                                         <  1:99518/178‑1 (MQ=255)
                                                                                                                                                                                                                ccTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGCCTGGGTACTGGGCGTGAAATCCATGCTTGGCGCCATTTTAATTCTGTTTTTTGCCTGGACAATCAATAACg                                                       <  1:668784/228‑1 (MQ=255)
                                                                                                                                                                                                                      cAGCTAGCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGCCTGGGTAc                                                                                                                      >  1:878863/1‑159 (MQ=255)
                                                                                                                                                                                                                            gCGGAAAGCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAgg                                                                                                                                                                                                                              >  1:686724/1‑49 (MQ=255)
                                                                                                                                                                                                                                   gCCGTTCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGCCTGGGTACTGGGCGTGAAATCCATGCTTGGCGCCATTTTAATTCTGTTTTTTGCCTGGACAATCAATAACGTGGTCAGCGatat                                          <  1:422968/222‑1 (MQ=255)
                                                                                                                                                                                                                                        tCAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGCCTGGGTACTGGGCGTGAAATCCATGCTTGGCGCCATTTTAATTCTGTTTTTTGCCTGGACAATCAATAACGTGGTCAGCGATATAAAAACCGGCACTTATTTAGCCTCCTTGGTATcc        <  1:884746/251‑1 (MQ=255)
                                                                                                                                                                                                                                         cAGTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGCCTGGGTACTGGGCGTGAAATCCATGCTTGGCGCCATTTTAATTCTGTTTTTTGCCTGGACAATCAATAACGTGGTCAGCGa                                             <  1:477269/213‑1 (MQ=255)
                                                                                                                                                                                                                                           gTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGCCTGGGTACTGGGCGTGAAATCCATGCTTGGCGCCATTTTGATTCTGTTTTTTGCCTGGACAATCAATAACGTGGTCAGCGATATaa                                        >  1:435107/1‑216 (MQ=255)
                                                                                                                                                                                                                                           gTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGCCTGGGTACTGGGCGTGAAATCCATGCTTGGCGCCATTTTAATTCTGTTTTTTGCCTGGACAATCAATAACGTGGTCAGCGATATaa                                        <  1:379800/216‑1 (MQ=255)
                                                                                                                                                                                                                                           gTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGCCTGGGTACTGGGCGTGAAATCCATGCTTGGCGCCATTTTAATTCTGTTTTTTGCCTGGACAATCAATAACGTGGTCAGCGATATAAAAAcc                                   <  1:794253/221‑1 (MQ=255)
                                                                                                                                                                                                                                           gTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGCCTGGGTACTGGGCGTGAAATCCATGCTTGGCGCCATTTTAATTCTGTTTTTTGCCTGGACAATCAATAACGTGGTCAGCGATATAAAAACCGGCACTTATTTAGcc                    <  1:758869/236‑1 (MQ=255)
                                                                                                                                                                                                                                           gTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGCCTGGGTACTGGGCGTGAAATCCATGCTTGGCGCCATTTTAATTCTGTTTTTTGCCTGGACAATCAATAACGTGGTCAGCGATATAAAAACCGGCACTTATTTAGCCTCCTTGGTATCCTa      >  1:880552/1‑250 (MQ=255)
                                                                                                                                                                                                                                           gTATACTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGCCTGGGTACTGGGCGTGAAATCCATGCTTGGCGCCATTTTAATTCTGTTTTTTGCCTGGACAATCAATAACGTGGTCAGCGATATAAAAACCGGCACTTATTTAGCCTCCTTGGTATCCTa      >  1:892699/1‑250 (MQ=255)
                                                                                                                                                                                                                                           gTATACTTGATGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGCCTGGGTACTGGGCGTGAAATCCATGCTTGGCGCCATTTTAATTCTGTTTTTTGCCTGGACAATCAATAACGTGGTCAGCGATATAAAAACCGGCACTTATTTAGCCTCCTTGGTATc         <  1:3564/247‑1 (MQ=255)
                                                                                                                                                                                                                                              taCTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGCCTGGGTACTGGGCGTGAAATCCATGCTTGGCGCCATTTTAATTCTGTTTTTTGCCTGGACAATCAATAACGTGGTCAGCGATATAAAAACCGGCACTTATTTAGCCTCCTTGGTATCCTACGcc  <  1:719057/251‑1 (MQ=255)
                                                                                                                                                                                                                                               aCTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGCCTGGGTACTggg                                                                                                                  <  1:279116/138‑1 (MQ=255)
                                                                                                                                                                                                                                               aCTTGGTGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGCCTGGGTACTGGGCGTGAAATCCATGCTTGGCGCCATTTTAATTCTGTTTTTTGCCTGGACAATCAATAACg                                                       >  1:333837/1‑197 (MQ=255)
                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    
ATTTTCTCTATTTTAATGGTGTTCTTCGTAGCCTATTTCAGCTTTGACATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGAAACAGAAAATACGCAAGGAGAAGCGCTTTCAGGCATAAACGGTCATGTACGCAATTTAGTATTACCAATTATCACCCTTATTATCATAACAATTTCTGCCATGCTATACACTGGTAACCTAGCCTTAACAGCTAGCGGAAAGCCGTTCAGTATACTTGATGCCTTTGAAAATACCACCGTAGGTGTATCTTTAGTCACCGGTGGTGTAAGTGCGGTGATTATTGCGTCCATTTGTATTTTATCCGATCGTCTGGTAAGCCTGCATGAATATGTCAAAGCCTGGGTACTGGGCGTGAAATCCATGCTTGGCGCCATTTTAATTCTGTTTTTTGCCTGGACAATCAATAACGTGGTCAGCGATATAAAAACCGGCACTTATTTAGCCTCCTTGGTATCCTACGCC  >  1/1201107‑1201595

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑