Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,201,053	A→G	S193G (AGC→GGC)	unknown →	Na+/H+ antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,201,053	0	A	G	100.0%	173.4 / NA	61	S193G (AGC→GGC)	unknown	Na+/H+ antiporter
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (27/34);  total (27/34)

TGGGGCAAAATTGGTTGCCGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCCACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAAGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGTTCTTCGTAGCCTATTTCAGCTTTGACATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGAAACAGAAAATACGCAAGGAGAAGCGCTTTCAGGCATAAACGGTCATGTACGCAATTTAGTATTACCAATTATCACCCTTATTATCATAACAATTTCTGCCATG  >  1/1200812‑1201295                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   tGGGGCAAAATTGGTTGCCGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCAc                                                                                                                                                                                                                                           <  1:828270/251‑1 (MQ=255)                tGCCGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTa                                                                                                                                                                                                                            <  1:141334/251‑1 (MQ=255)                  ccGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTAt                                                                                                                                                                                                                           <  1:267433/250‑1 (MQ=255)                       tCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAAt                                                                                                                                                                                                                     >  1:755646/1‑251 (MQ=255)                             gTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTg                                                                                                                                                                                                                >  1:666178/1‑250 (MQ=255)                                  cGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATAAGTGCTATGa                                                                                                                                                                                                          <  1:583217/251‑1 (MQ=255)                                    tCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAAc                                                                                                                                                                                                        <  1:735558/251‑1 (MQ=255)                                    tCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAAc                                                                                                                                                                                                        <  1:640022/251‑1 (MQ=255)                                               gATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGGATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTAt                                                                                                                                                                                             >  1:456778/1‑251 (MQ=255)                                                    cTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTAtttt                                                                                                                                                                                          >  1:441230/1‑249 (MQ=255)                                                     tATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACt                                                                                                                                                                                                       >  1:69527/1‑235 (MQ=255)                                                             caGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTaa                                                                                                                                                                               >  1:323322/1‑251 (MQ=255)                                                               gTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTa                                                                                                                                                                                                                            >  1:654523/1‑204 (MQ=255)                                                               gTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATg                                                                                                                                                                             >  1:618542/1‑251 (MQ=255)                                                                    gCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCAATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATgg                                                                                                                                                                            <  1:123647/247‑1 (MQ=255)                                                                        tAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGttcttc                                                                                                                                                                    <  1:181227/251‑1 (MQ=255)                                                                           gTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGa                                                                                                                                                                                                          <  1:31345/210‑1 (MQ=255)                                                                           gTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGc                                                                                                                                                                                                <  1:489013/220‑1 (MQ=255)                                                                              cTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTAttt                                                                                                                                                                                           <  1:164789/222‑1 (MQ=255)                                                                              cTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGTTCTTCGTAGc                                                                                                                                                               <  1:666533/250‑1 (MQ=255)                                                                                aTTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGTTCTTCGTAGCCTa                                                                                                                                                            >  1:749266/1‑251 (MQ=255)                                                                                                     aaaTTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGTTCTTCGTAGCCTATTTCAGCTTTGACATCGGTcc                                                                                                                                       <  1:725844/251‑1 (MQ=255)                                                                                                       aTTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTctct                                                                                                                                                                                      >  1:370966/1‑202 (MQ=255)                                                                                                                        ccAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGTTCTTCGTAGCCTATTTCAGCTTTGACATCGGTCCCATGGCAAGACATGAAAgg                                                                                                                    <  1:528510/251‑1 (MQ=255)                                                                                                                               aGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAAc                                                                                                                                                                                                        <  1:199632/160‑1 (MQ=255)                                                                                                                                      atTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGttctt                                                                                                                                                                     <  1:91953/188‑1 (MQ=255)                                                                                                                                       ttCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGTTCTTCGTAGCCTATTTCAGCTTTGACATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGAAACa                                                                                                     >  1:429141/1‑251 (MQ=255)                                                                                                                                              ctctaCCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGTTCTTCGTAGCCTATTTCAGCTTTGACATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGa                                                                                                         >  1:862202/5‑240 (MQ=255)                                                                                                                                               cttggtaaagctgcGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGTTCTTCGTAGCCTATTTCAGCTTTGACATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGAAACAGAAAATa                                                                                              >  1:728449/14‑250 (MQ=255)                                                                                                                                                        gCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTa                                                                                                                                                                                              >  1:348856/1‑145 (MQ=255)                                                                                                                                                              aTGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAAc                                                                                                                                                                                                        >  1:279181/1‑129 (MQ=255)                                                                                                                                                                 tgCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGTTCTTCGTAGCCTATTTCAGCTTTGACATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGAAACAGAAAATACGCAAGGAGAAGCGCttt                                                                            >  1:686997/1‑250 (MQ=255)                                                                                                                                                                  gCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTa                                                                                                                                                                                              <  1:848092/135‑1 (MQ=255)                                                                                                                                                                     tACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGc                                                                                                                                                                                                                        <  1:862232/106‑1 (MQ=255)                                                                                                                                                                           tGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGTTCTTCGTAGCCTATTTCAGCTTTGACATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGAAACAGAAAATACGCAAGGAGAAGCGCTTTCAGGCATAAAc                                                                 <  1:731108/251‑1 (MQ=255)                                                                                                                                                                                gTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGTTCTTCGTAGCCTATTTCAGCTTTGACATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGAAACAGAAAATACGCAAGGAGAAGCGCTTTCAGGCATAAACGGTCa                                                            >  1:683470/1‑251 (MQ=255)                                                                                                                                                                                     tAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGTTCTTCGTAGCCTATTTCAGCTTTGACATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGAAACAGAAAATACGCAAGGAGAAGCGCTTTCAGGCATAAACGGTCATGTAc                                                       <  1:154786/251‑1 (MQ=255)                                                                                                                                                                                             gcgcATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGTTCTTCGTAGCCTATTTCAGCTTTGACATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGAAACAGAAAATACGCAAGGAGAAGCGCTTTCAGGCATAAACGGTCATGTACGCAAttt                                                <  1:512771/250‑1 (MQ=255)                                                                                                                                                                                                cATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGTTCTTCGTAGCCTATTTCAGCTTTGACATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGAAACAGAAAATACGCAAGga                                                                                      <  1:804638/209‑1 (MQ=255)                                                                                                                                                                                                cATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGTTCTTCGTAGCCTATTTCAGCTTTGACATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGAAACAGAAAATACGCAAGGAGAAGCGCTTTCAGGCATAAACGGTCATGTACGCAATTTAGTa                                            >  1:487495/1‑251 (MQ=255)                                                                                                                                                                                                cATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGTTCTTCGTAGCCTATTTCAGCTTTGACATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGAAACAGAAAATACGCAAGGAGAAGCGCTTTCAGGCATAAACGGTCATGAACGCAATTTAGTa                                            >  1:84073/1‑251 (MQ=255)                                                                                                                                                                                                    tatCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATg                                                                                                                                                                                                                    <  1:423250/79‑1 (MQ=255)                                                                                                                                                                                                    tatCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGTTCTTCGTAGCCTATTTCAGCTTTGACATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGAAACAGAAAATACGCAAGGAGAAGCGCTTTCAGGCATAAACGGTCATGTAc                                                       >  1:217805/1‑236 (MQ=255)                                                                                                                                                                                                     atCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGTTCTTCGTAGCCTATTTCAGCTTTGACATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGAAACAGAAAATACGCAAGGAGAAGCGCTTTCAGGCATAAACGGTCATGTAc                                                       <  1:77836/235‑1 (MQ=255)                                                                                                                                                                                                     atCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGTTCTTCGTAGCCTATTTCAGCTTTGACATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGAAACAGAAAATACGCAAGGAGAAGCGCTTTCAGGCATAAACGGTCATGTACGCAATTTAGTATTAcc                                       <  1:653594/251‑1 (MQ=255)                                                                                                                                                                                                                 gTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGTTCTTCGTAGc                                                                                                                                                               <  1:827779/119‑1 (MQ=255)                                                                                                                                                                                                                      tGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGTTCTTCGTAGCCTATTTCAGCTTTGACATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGAAACAGAAAATACGCAAGGAGAAGCGCTTTCAGGCATAAACGGTCATGTACGCAATTTAGTATTACCAATTATCACCCttatta                      <  1:308010/251‑1 (MQ=255)                                                                                                                                                                                                                      tGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGTTCTTCGTAGCCTATTTCAGCTTTGACATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGAAACAGAAAATACGCAAGGAGAAGCGCTTTCAGGCATAAACGGTCATGTACGCAATTTAGTATTACCAATTATCACCCttatta                      <  1:67786/251‑1 (MQ=255)                                                                                                                                                                                                                      tGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGTTCTTCGTAGCCTATTTCAGCTTTGACATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGAAACAGAAAATACGCAAGGAGAAGCGCTTTCAGGCATAAACGGTCATGTACGCAATTTAGTATTACCAATTATCACCCttatta                      <  1:813313/251‑1 (MQ=255)                                                                                                                                                                                                                      tGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGTTCTTCGTAGCCTATTTCAGCTTTGACATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGAAACAGAAAATACGCAAGGAGAAGCGCTTTCAGGCATAAACGGTCATGTACGCAATTTAGTATTACCAATTATCACCCttatta                      <  1:50109/251‑1 (MQ=255)                                                                                                                                                                                                                        gCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGTTCTTCGTAGCCTATTTCAGCTTTGACATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGAAACAGAAAATACGCAAGGAGAAGCGCTTTCAGGCATAAACGGTCATGTACGCAATTTAGTATTACCAATTATCACCCTtattat                     <  1:303463/250‑1 (MQ=255)                                                                                                                                                                                                                         cTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGTTCTTCGTAGCCTATTTCAGCTTTGACATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGAAACAGAAAATACGCAAGGAGAAGCGCTTTCAGGCATAAACGGTCATGTACGCAATTTAGt                                             >  1:612588/1‑225 (MQ=255)                                                                                                                                                                                                                                  cACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAAc                                                                                                                                                                                                        <  1:24164/61‑1 (MQ=255)                                                                                                                                                                                                                                  cACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGTTCTTCGTAGCCTATTTCAGCTTTGACATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGAAACAGAAAATACGCAAGGAGAAGCGCTTTCAGGCATAAACGGTCATGTACGCAATTTAGTATTAcc                                       >  1:76254/1‑222 (MQ=255)                                                                                                                                                                                                                                  cACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGTTCTTCGTAGCCTATTTCAGCTTTGACATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGAAACAGAAAATACGCAAGGAGAAGCGCTTTCAGGCATAAACGGTCATGTACGCAATTTAGTATTACCAATTATCACCCTTATTATCATAACAAttt          >  1:710569/1‑251 (MQ=255)                                                                                                                                                                                                                                   aCTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTtctc                                                                                                                                                                                       <  1:260194/77‑1 (MQ=255)                                                                                                                                                                                                                                        ttCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGTTCTTCGTAGCCTATTTCAGCTTTGACATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGaa                                                                                                        >  1:89192/1‑151 (MQ=255)                                                                                                                                                                                                                                        ttCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGTTCTTCGTAGCCTATTTCAGCTTTGACATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGAAACAGAAAATACGCAAGGAGAAGCGCTTTCAGGCATAAACGGTCATGTACGCAATTTAGt                                             >  1:868621/1‑210 (MQ=255)                                                                                                                                                                                                                                            aTCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGTTCTTCGTAGCCTATTTCAGCTTTGACATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGAAACAGAAAATACGCAAGGAGAAGCGCTTTCAGGCATAAACGGTCATGTACGCAATTTAGTATTACCAATTATCACCCttatta                      >  1:522616/1‑229 (MQ=255)                                                                                                                                                                                                                                            aTCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGTTCTTCGTAGCCTATTTCAGCTTTGACATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGAAACAGAAAATACGCAAGGAGAAGCGCTTTCAGGCATAAACGGTCATGTACGCAATTTAGTATTACCAATTATCACCCttatta                      <  1:201101/229‑1 (MQ=255)                                                                                                                                                                                                                                            aTCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGTTCTTCGTAGCCTATTTCAGCTTTGACATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGAAACAGAAAATACGCAAGGAGAAGCGCTTTCAGGCATAAACGGTCATGTACGCAATTTAGTATTACCAATTATCACCCTTATTATCATAACAATTTCTGCCATg  >  1:792228/1‑249 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                   TGGGGCAAAATTGGTTGCCGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCCACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAAGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGTTCTTCGTAGCCTATTTCAGCTTTGACATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGAAACAGAAAATACGCAAGGAGAAGCGCTTTCAGGCATAAACGGTCATGTACGCAATTTAGTATTACCAATTATCACCCTTATTATCATAACAATTTCTGCCATG  >  1/1200812‑1201295

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑