Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,200,956	C→T	S160S (TCC→TCT)	unknown →	Na+/H+ antiporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,200,956	0	C	T	100.0%	186.2 / NA	66	S160S (TCC→TCT)	unknown	Na+/H+ antiporter
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (31/35);  total (31/35)

CTTTTCTTATTATTACTCGGAATTTTGACCGCCCTTTTAAGTATGTCCGGCAGTAACCGCGCCTTTGCAGAATGGTCACAAAAACAGATTAAAGATCGCCGTGGGGCAAAATTGGTTGCCGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCCACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAAGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGTTCTTCGTAGCCTATTTCAGCTTTGACATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGAAACA  >  1/1200711‑1201196                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 cttttcttATTATTACTCGGAATTTTGACCGCCCTTTTAAGTATGTCCGGCAGTAACCGCGCCTTTGCAGAATGGTCACAAAAACAGATTAAAGATCGCCGTGGGGCAAAATTGGTTGCCGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGc                                                                                                                                                                                                                                             <  1:660862/251‑1 (MQ=255)                    gAATTTTGACCGCCCTTTTAAGTATGTCCGGCAGTAACCGCGCCTTTGCAGAATGGTCACAAAAACAGATTAAAGATCGCCGTGGGGCAAAATTGGTTGCCGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGa                                                                                                                                                                                                                                     >  1:469996/1‑240 (MQ=255)                    gAATTTTGACCGCCCTTTTAAGTATGTCCGGCAGTAACCGCGCCTTTGCAGAATGGTCACAAAAACAGATTAAAGATCGCCGTGGGGCAAAATTGGTTGCCGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACt                                                                                                                                                                                                                           <  1:708065/250‑1 (MQ=255)                     aaTTTTGACCGCCCTTTTAAGTATGTCCGGCAGTAACCGCGCCTTTGCAGAATGGTCACAAAAACAGATTAAAGATCGCCGTGGGGCAAAATTGGTTGCCGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTc                                                                                                                                                                                                                          <  1:600104/250‑1 (MQ=255)                     aaTTTTGACCGCCCTTTTAAGTATGTCCGGCAGTAACCGCGCCTTTGCAGAATGGTCACAAAAACAGATTAAAGATCGCCGTGGGGCAAAATTGGTTGCCGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCa                                                                                                                                                                                                                         <  1:118393/251‑1 (MQ=255)                     aaTTTTGACCGCCCTTTTAAGTATGTCCGGCAGTAACCGCGCCTTTGCAGAATGGTCACAAAAACAGATTAAAGATCGCCGTGGGGCAAAATTGGTTGCCGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCa                                                                                                                                                                                                                         <  1:81701/251‑1 (MQ=255)                                cccTTTTAAGTATGTCCGGCAGTAACCGCGCCTTTGCAGAATGGTCACAAAAACAGATTAAAGATCGCCGTGGGGCAAAATTGGTTGCCGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACt                                                                                                                                                                                                                           >  1:194475/1‑238 (MQ=255)                                cccTTTTAAGTATGTCCGGCAGTAACCGCGCCTTTGCAGAATGGTCACAAAAACAGATTAAAGATCGCCGTGGGGCAAAATTGGTTGCCGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTAt                                                                                                                                                                                                                >  1:705611/1‑249 (MQ=255)                                  cTTTTAAGTATGTCCGGCAGTAACCGCGCCTTTGCAGAATGGTCACAAAAACAGATTAAAGATCGCCGTGGGGCAAAATTGGTTGCCGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGc                                                                                                                                                                                                                                             >  1:205005/1‑218 (MQ=255)                                  cTTTTAAGTATGTCCGGCAGTAACCGCGCCTTTGCAGAATGGTCACAAAAACAGATTAAAGATCGCCGTGGGGCAAAATTGGTTGCCGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAg                                                                                                                                                                                                            >  1:303787/1‑251 (MQ=255)                                   ttttAAGTATGTCCGGCAGTAACCGCGCCTTTGCAGAATGGTCACAAAAACAGATTAAAGATCGCCGTGGGGCAAAATTGGTTGCCGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGAtg                                                                                                                                                                                                                                   >  1:780688/1‑227 (MQ=255)                                       aaGTATGTCCGGCAGTAACCGCGCCTTTGCAGAATGGTCACAAAAACAGATTAAAGATCGCCGTGGGGCAAAATTGGTTGCCGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGa                                                                                                                                                                                                                                     <  1:878053/221‑1 (MQ=255)                                        aGTATGTCCGGCAGTAACCGCGCCTTTGCAGAATGGTCACAAAAACAGATTAAAGATCGCCGTGGGGCAAAATTGGTTGCCGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGc                                                                                                                                                                                                                                             >  1:26580/1‑212 (MQ=255)                                             gTCCGGCAGTAACCGCGCCTTTGCAGAATGGTCACAAAAACAGATTAAAGATCGCCGTGGGGCAAAATTGGTTGCCGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTAt                                                                                                                                                                                                                >  1:818398/1‑236 (MQ=255)                                             gTCCGGCAGTAACCGCGCCTTTGCAGAATGGTCACAAAAACAGATTAAAGATCGCCGTGGGGCAAAATTGGTTGCCGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCat                                                                                                                                                                                                 >  1:252489/1‑251 (MQ=255)                                                      tAACCGCGCCTTTGCAGAATGGTCACAAAAACAGATTAAAGATCGCCGTGGGGCAAAATTGGTTGCCGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCa                                                                                                                                                                                           <  1:523750/248‑1 (MQ=255)                                                      tAACCGCGCCTTTGCAGAATGGTCACAAAAACAGATTAAAGATCGCCGTGGGGCAAAATTGGTTGCCGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCa                                                                                                                                                                                           <  1:407423/248‑1 (MQ=255)                                                        aCCGCGCCTTTGCAGAATGGTCACAAAAACAGATTAAAGATCGCCGTGGGGCAAAATTGGTTGCCGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTAcc                                                                                                                                                                                      <  1:126930/251‑1 (MQ=255)                                                              ccTTTGCAGAATGGTCACAAAAACAGATTAAAGATCGCCGTGGGGCAAAATTGGTTGCCGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTc                                                                                                                                                                                <  1:629853/251‑1 (MQ=255)                                                                           tggtcaAAAACAGATTAAAGATCGCCGTGGGGCAAAATTGGTTGCCGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAg                                                                                                                                                                   <  1:122335/247‑1 (MQ=255)                                                                                       gATTAAAGATCGCCGTGGGGCAAAATTGGTTGCCGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGt                                                                                                                                                                                                           <  1:62995/199‑1 (MQ=255)                                                                                       gATTAAAGATCGCCGTGGGGCAAAATTGGTTGCCGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCa                                                                                                                                                                                           >  1:232581/1‑215 (MQ=255)                                                                                       gATTAAAGATCGCCGTGGGGCAAAATTGGTTGCCGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTcc                                                                                                                                                        >  1:846372/1‑250 (MQ=255)                                                                                       gATTAAAGATCGCCGTGGGGCAAAATTGGTTGCCGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCa                                                                                                                                                       >  1:297932/1‑251 (MQ=255)                                                                                        aTTAAAGATCGCCGTGGGGCAAAATTGGTTGCCGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTc                                                                                                                                                         <  1:331134/248‑1 (MQ=255)                                                                                           aaaGATCGCCGTGGGGCAAAATTGGTTGCCGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTc                                                                                                                                                                                                                          >  1:141335/1‑180 (MQ=255)                                                                                           aaaGATCGCCGTGGGGCAAAATTGGTTGCCGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCt                                                                                                                                                                                                              <  1:600736/192‑1 (MQ=255)                                                                                           aaaGATCGCCGTGGGGCAAAATTGGTTGCCGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATca                                                                                                                                                    >  1:854208/1‑250 (MQ=255)                                                                                             aGATCGCCGTGGGGCAAAATTGGTTGCCGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATcacag                                                                                                                                                 >  1:603197/1‑250 (MQ=255)                                                                                              gATCGCCGTGGGGCAAAATTGGTTGCCGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTAcc                                                                                                                                                                                      >  1:515239/1‑213 (MQ=255)                                                                                                      tGGGGCAAAATTGGTTGCCGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCAc                                                                                                                                        <  1:828270/251‑1 (MQ=255)                                                                                                        gggCAAAATTGGTTGCCGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATcac                                                                                                                                                   <  1:592070/238‑1 (MQ=255)                                                                                                          gCAAAATTGGTTGCCGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGc                                                                                                                                                                          >  1:62265/1‑213 (MQ=255)                                                                                                             aaaTTGGTTGCCGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGgcg                                                                                                                                                                                                    <  1:321625/184‑1 (MQ=255)                                                                                                                     tGCCGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTa                                                                                                                         <  1:141334/251‑1 (MQ=255)                                                                                                                       ccGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTAt                                                                                                                        <  1:267433/250‑1 (MQ=255)                                                                                                                            tCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAAt                                                                                                                  >  1:755646/1‑251 (MQ=255)                                                                                                                                  gTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTg                                                                                                             >  1:666178/1‑250 (MQ=255)                                                                                                                                       cGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATAAGTGCTATGa                                                                                                       <  1:583217/251‑1 (MQ=255)                                                                                                                                         tCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAAc                                                                                                     <  1:735558/251‑1 (MQ=255)                                                                                                                                         tCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAAc                                                                                                     <  1:640022/251‑1 (MQ=255)                                                                                                                                           aCCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCg                                                                                                                                                                                                                    >  1:360701/1‑138 (MQ=255)                                                                                                                                             cTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGc                                                                                                                                                                                                                                             <  1:258371/111‑1 (MQ=255)                                                                                                                                                    gATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGGATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTAt                                                                                          >  1:456778/1‑251 (MQ=255)                                                                                                                                                         cTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCAt                                                                                                                                                                                          <  1:456306/150‑1 (MQ=255)                                                                                                                                                         cTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTAtttt                                                                                       >  1:441230/1‑249 (MQ=255)                                                                                                                                                          tATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACt                                                                                                    >  1:69527/1‑235 (MQ=255)                                                                                                                                                            tttCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGc                                                                                                                                                                  <  1:700542/171‑1 (MQ=255)                                                                                                                                                                  caGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTaa                                                                            >  1:323322/1‑251 (MQ=255)                                                                                                                                                                    gTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTa                                                                                                                         >  1:654523/1‑204 (MQ=255)                                                                                                                                                                    gTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATg                                                                          >  1:618542/1‑251 (MQ=255)                                                                                                                                                                         gCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATcacaggc                                                                                                                                               >  1:378072/1‑174 (MQ=255)                                                                                                                                                                         gCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCAATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATgg                                                                         <  1:123647/247‑1 (MQ=255)                                                                                                                                                                             tAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCa                                                                                                                                                                                                                         >  1:354294/1‑99 (MQ=255)                                                                                                                                                                             tAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGttcttc                                                                 <  1:181227/251‑1 (MQ=255)                                                                                                                                                                                gTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGa                                                                                                       <  1:31345/210‑1 (MQ=255)                                                                                                                                                                                gTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGc                                                                                             <  1:489013/220‑1 (MQ=255)                                                                                                                                                                                   cTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTAttt                                                                                        <  1:164789/222‑1 (MQ=255)                                                                                                                                                                                   cTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGTTCTTCGTAGc                                                            <  1:666533/250‑1 (MQ=255)                                                                                                                                                                                     aTTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGTTCTTCGTAGCCTa                                                         >  1:749266/1‑251 (MQ=255)                                                                                                                                                                                                          aaaTTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGTTCTTCGTAGCCTATTTCAGCTTTGACATCGGTcc                                    <  1:725844/251‑1 (MQ=255)                                                                                                                                                                                                            aTTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTctct                                                                                   >  1:370966/1‑202 (MQ=255)                                                                                                                                                                                                                             ccAAATTAGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGTTCTTCGTAGCCTATTTCAGCTTTGACATCGGTCCCATGGCAAGACATGAAAgg                 <  1:528510/251‑1 (MQ=255)                                                                                                                                                                                                                                    aGCTTATATTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAAc                                                                                                     <  1:199632/160‑1 (MQ=255)                                                                                                                                                                                                                                           atTCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGttctt                                                                  <  1:91953/188‑1 (MQ=255)                                                                                                                                                                                                                                            ttCTTGACTCTACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAGGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGTTCTTCGTAGCCTATTTCAGCTTTGACATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGAAACa  >  1:429141/1‑251 (MQ=255)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                 CTTTTCTTATTATTACTCGGAATTTTGACCGCCCTTTTAAGTATGTCCGGCAGTAACCGCGCCTTTGCAGAATGGTCACAAAAACAGATTAAAGATCGCCGTGGGGCAAAATTGGTTGCCGCTTCGTTAGTCTTCGTCACCTTCATCGATGACTATTTCCACAGTTTAGCCGTAGGTGCTATTGCCCGCCCGATTACCGATAAATTCAAAGTCTCCCGCGCCAAATTAGCTTATATTCTTGACTCCACCGCTGCACCGATGTGCGTACTCATGCCGGTATCTAGTTGGGGCGCATATATCATTACCTTAGTCGCTGGCTTATTAGCCACTTATTCCATCACAAGCTACTCACCAATCGGTGCATTTATGGCAATGAGTGCTATGAACTATTACGCTATTTTCTCTATTTTAATGGTGTTCTTCGTAGCCTATTTCAGCTTTGACATCGGTCCCATGGCAAGACATGAAAGGCTGGCATTGGAAACA  >  1/1200711‑1201196

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑