Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,196,194	A→G	R548R (CGT→CGC)	unknown ←	Cell division‑associated, ATP‑dependent zinc metalloprotease FtsH

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,196,194	0	A	G	100.0%	199.8 / NA	74	R548R (CGT→CGC)	unknown	Cell division‑associated, ATP‑dependent zinc metalloprotease FtsH
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (33/41);  total (33/41)

GCCCTGCGCATCATCTGCCATTTTTGGCTCTTCAGCTTTCGGCTGCTGAGGTTCGGCTTTATCTGTATTCTTTGGTTCTTCCCAACCTGGAGGCGGTGTCACTGGTTGACGATTCATTAACTGCTTGATTTGTTCTTCTTCAATAGTTTCGTATTTCACCAGCGCATCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCACGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCACTGAGTCACCATATTACGGGCGATGTTGGTTGCCACTTTAATATCATTGGACGCCCCGGTAGAAATATTTTCTTCACCGTAAATCAAATCTTCTGCCAAACGACCTGCGTATAAC  >  1/1195951‑1196440                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       gCCCTGCGCATCATCTGCCATTTTTGGCTCTTCAGCTTTCGGCTGCTGAGGTTCAGCTTTATCTGTATTCTTTGGTTCTTCCCAACCTGGAGGCGGTGTCACTGGTTGACGATTCATTAACTGCTTGATTTGTTCTTCTTCAATAGTTTCGTATTTCACCAGCGCATCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACtt                                                                                                                                                                                                                                                 >  1:691323/1‑251 (MQ=255)    cTGCGCATCATCTGCCATTTTTGGCTCTTCAGCTTTCGGCTGCTGAGGTTCAGCTTTATCTGTATTCTTTGGTTCTTCCCAACCTGGAGGCGGTGTCACTGGTTGACGATTCATTAACTGCTTGATTTGTTCTTCTTCAATAGTTTCGTATTTCACCAGCGCATCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTc                                                                                                                                                                                                                                                >  1:839161/1‑249 (MQ=255)                 gCCATTTTTGGCTCTTCAGCTTTCGGCTGCTGAGGTTCAGCTTTATCTGTATTCTTTGGTTCTTCCCAACCTGGAGGCGGTGTCACTGGTTGACGATTCATTAACTGCTTGATTTGTTCTTCTTCAATAGTTTCGTATTTCACCAGCGCATCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACAtg                                                                                                                                                                                                                                 >  1:164982/1‑251 (MQ=255)                                   gCTTTCGGCTGCTGAGGTTCAGCTTTATCTGTATTCTTTGGTTCTTCCCAACCTGGAGGCGGTGTCACTGGTTGACGATTCATTAACTGCTTGATTTGTTCTTCTTCAATAGTTTCGTATTTCACCAGCGCATCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACAt                                                                                                                                                                                                               >  1:388374/1‑251 (MQ=255)                                      ttCGGCTGCTGAGGTTCAGCTTTATCTGTATTCTTTGGTTCTTCCCAACCTGGAGGCGGTGTCACTGGTTGACGATTCATTAACTGCTTGATTTGTTCTTCTTCAATAGTTTCGTATTTCACCAGCGCATCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACAtgtg                                                                                                                                                                                                            <  1:735712/251‑1 (MQ=255)                                      ttCGGCTGCTGAGGTTCAGCTTTATCTGTATTCTTTGGTTCTTCCCAACCTGGAGGCGGTGTCACTGGTTGACGATTCATTAACTGCTTGATTTGTTCTTCTTCAATAGTTTCGTATTTCACCAGCGCATCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACAtgtg                                                                                                                                                                                                            <  1:753154/251‑1 (MQ=255)                                           ctgctgAGGTTCAGCTTTATCTGTATTCTTTGGTTCTTCCCAACCTGGAGGCGGTGTCACTGGTTGACGATTCATTAACTGCTTGATTTGTTCTTCTTCAATAGTTTCGTATTTCACCAGCGCATCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTgg                                                                                                                                                                                                       <  1:202221/251‑1 (MQ=255)                                                        gCTTTATCTGTATTCTTTGGTTCTTCCCAACCTGGAGGCGGTGTCACTGGTTGACGATTCATTAACTGCTTGATTTGTTCTTCTTCAATAGTTTCGTATTTCACCAGCGCATCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACAtgtg                                                                                                                                                                                                            <  1:337451/233‑1 (MQ=255)                                                        gCTTTATCTGTATTCTTTGGTTCTTCCCAACCTGGAGGCGGTGTCACTGGTTGACGATTCATTAACTGCTTGATTTGTTCTTCTTCAATAGTTTCGTATTTCACCAGCGCATCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGc                                                                                                                                                                                                      <  1:464157/239‑1 (MQ=255)                                                          tttATCTGTATTCTTTGGTTCTTCCCAACCTGGAGGCGGTGTCACTGGTTGACGATTCATTAACTGCTTGATTTGTTCTTCTTCAATAGTTTCGTATTTCACCAGCGCATCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGAt                                                                                                                                                                                                                                       <  1:274397/204‑1 (MQ=255)                                                                    ttCTTTGGTTCTTCCCAACCTGGAGGCGGTGTCACTGGTTGACGATTCATTAACTGCTTGATTTGTTCTTCTTCAATAGTTTCGTATTTCACCAGCGCATCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGaa                                                                                                                                                                              <  1:357850/251‑1 (MQ=255)                                                                    ttCTTTGGTTCTTCCCAACCTGGAGGCGGTGTCACTGGTTGACGATTCATTAACTGCTTGATTTGTTCTTCTTCAATAGTTTCGTATTTCACCAGCGCATCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGaa                                                                                                                                                                              <  1:708839/251‑1 (MQ=255)                                                                    ttCTTTGGTTCTTCCCAACCTGGAGGCGGTGTCACTGGTTGACGATTCATTAACTGCTTGATTTGTTCTTCTTCAATAGTTTCGTATTTCACCAGCGCATCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGaa                                                                                                                                                                              <  1:43127/251‑1 (MQ=255)                                                                      cTTTGGTTCTTCCCAACCTGGAGGCGGTGTCACTGGTTGACGATTCATTAACTGCTTGATTTGTTCTTCTTCAATAGTTTCGTATTTCACCAGCGCATCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCAt                                                                                                                                                                                                                      >  1:254410/1‑209 (MQ=255)                                                                      cTTTGGTTCTTCCCAACCTGGAGGCGGTGTCACTGGTTGACGATTCATTAACTGCTTGATTTGTTCTTCTTCAATAGTTTCGTATTTCACCAGCGCATCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCAt                                                                                                                                                                                                                      >  1:688685/1‑209 (MQ=255)                                                                       tttGGTTCTTCCCAACCTGGAGGCGGTGTCACTGGTTGACGATTCATTAACTGCTTGATTTGTTCTTCTTCAATAGTTTCGTATTTCACCAGCGCATCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATAc                                                                                                                                                                           <  1:756235/251‑1 (MQ=255)                                                                          ggTTCTTCCCAACCTGGAGGCGGTGTCACTGGTTGACGATTCATTAACTGCTTGATTTGTTCTTCTTCAATAGTTTCGTATTTCACCAGCGCATCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGccc                                                                                                                                                                                    >  1:836128/1‑239 (MQ=255)                                                                                                gtgtCACTGGTTGACGATTCATTAACTGCTTGATTTGTTCTTCTTCAATAGTTTCGTATTTCACCAGCGCATCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATAc                                                                                                                                                                           <  1:630750/226‑1 (MQ=255)                                                                                                 tgtCACTGGTTGACGATTCATTAACTGCTTGATTTGTTCTTCTTCAATAGTTTCGTATTTCACCAGCGCATCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGaa                                                                                                                                                 <  1:333414/251‑1 (MQ=255)                                                                                                  gtCACTGGTTGACGATTCATTAACTGCTTGATTTGTTCTTCTTCAATAGTTTCGTATTTCACCAGCGCATCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGaa                                                                                                                                                                              <  1:837053/221‑1 (MQ=255)                                                                                                           tGACGATTCATTAACTGCTTGATTTGTTCTTCTTCAATAGTTTCGTATTTCACCAGCGCATCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCTCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGACCGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAg                                                                                                                                       >  1:253350/1‑251 (MQ=255)                                                                                                                aTTCATTAACTGCTTGATTTGTTCTTCTTCAATAGTTTCGTATTTCACCAGCGCATCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGt                                                                                                                                      <  1:76397/247‑1 (MQ=255)                                                                                                                aTTCATTAACTGCTTGATTTGTTCTTCTTCAATAGTTTCGTATTTCACCAGCGCATCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTAt                                                                                                                                  >  1:50185/1‑251 (MQ=255)                                                                                                                 ttCATTAACTGCTTGATTTGTTCTTCTTCAATAGTTTCGTATTTCACCAGCGCATCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCgg                                                                                                                                                                                                                            <  1:877886/160‑1 (MQ=255)                                                                                                                 ttCATTAACTGCTTGATTTGTTCTTCTTCAATAGTTTCGTATTTCACCAGCGCATCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTAt                                                                                                                                  >  1:115851/1‑250 (MQ=255)                                                                                                                 ttCATTAACTGCTTGATTTGTTCTTCTTCAATAGTTTCGTATTTCACCAGCGCATCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTCTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTAt                                                                                                                                  >  1:73656/1‑250 (MQ=255)                                                                                                                    aTTAACTGCTTGATTTGTTCTTCTTCAATAGTTTCGTATTTCACCAGCGCATCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGaa                                                                                                                                                                                         >  1:458206/1‑192 (MQ=255)                                                                                                                               gATTTGTTCTTCTTCAATAGTTTCGTATTTCACCAGCGCATCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATAc                                                                                                                                                                           >  1:268187/1‑195 (MQ=255)                                                                                                                               gATTTGTTCTTCTTCAATAGTTTCGTATTTCACCAGCGCATCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCa                                                                                                                     >  1:500481/1‑249 (MQ=255)                                                                                                                                        ttcttcAATAGTTTCGTATTTCACCAGCGCATCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCACTGAGTCACCa                                                                                                          <  1:253779/251‑1 (MQ=255)                                                                                                                                          cttcAATAGTTTCGTATTTCACCAGCGCATCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGaa                                                                                                                                                                              >  1:399534/1‑181 (MQ=255)                                                                                                                                          cttcAATAGTTTCGTATTTCACCAGCGCATCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCAACGATCACATGTGCGGATTCATCGAACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGAACAAGTTTATCGGAGAAACCCCACTGAGTCACCata                                                                                                        >  1:860427/1‑251 (MQ=255)                                                                                                                                             cAATAGTTTCGTATTTCACCAGCGCATCTTTCATCGCGTGTAGAATATCCTTATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCACTGAGTCACCATATTa                                                                                                     <  1:204743/251‑1 (MQ=255)                                                                                                                                             cAATAGTTTCGTATTTCACCAGCGCATCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCACTGAGTCACCATATTa                                                                                                     <  1:827743/251‑1 (MQ=255)                                                                                                                                              aaTAGTTTCGTATTTCACCAGCGCATCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCACTGAGTCACCATATTAc                                                                                                    <  1:597154/251‑1 (MQ=255)                                                                                                                                              aaTAGTTTCGTATTTCACCAGCGCATCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCACTGAGTCACCATATTAc                                                                                                    <  1:344319/251‑1 (MQ=255)                                                                                                                                                  gTTTCGTATTTCACCAGCGCATCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCACTg                                                                                                                  <  1:879471/233‑1 (MQ=255)                                                                                                                                                  gTTTCGTATTTCACCAGCGCATCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCACTGAGTCACCATATTACggg                                                                                                 >  1:17452/1‑250 (MQ=255)                                                                                                                                                          tttCACCAGCGCATCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCACTGAGTCACCATATTACGGGCGATgttgg                                                                                        >  1:698961/1‑251 (MQ=255)                                                                                                                                                            tCACCAGCGCATCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCACTGAGTCACCATATTACGGGCGATgttggtt                                                                                      <  1:751203/251‑1 (MQ=255)                                                                                                                                                            tCACCAGCGCATCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCACTGAGTCACCATATTACGGGCGATgttggtt                                                                                      <  1:728343/251‑1 (MQ=255)                                                                                                                                                                      aTCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGaa                                                                                                                                                                              <  1:221323/153‑1 (MQ=255)                                                                                                                                                                       tCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCGCAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCACTGAGTCACCATATTa                                                                                                     <  1:807719/225‑1 (MQ=255)                                                                                                                                                                       tCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCACTGAGTCACCATATTAc                                                                                                    <  1:603617/226‑1 (MQ=255)                                                                                                                                                                       tCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCACTGAGTCACCATATTACGGGCGATgttggtt                                                                                      <  1:879790/240‑1 (MQ=255)                                                                                                                                                                         tttCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCACTGAGTCACCATATTACGGGCGATGTTGGTTGCCACTTTAatat                                                                         <  1:680831/251‑1 (MQ=255)                                                                                                                                                                                 cgTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCACTg                                                                                                                  >  1:242353/1‑202 (MQ=255)                                                                                                                                                                                 cgTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCACTGAGTCACCATATTACGGGCGATGTTGGTTGCCACTTTAATATCATTGGAc                                                                 <  1:324593/251‑1 (MQ=255)                                                                                                                                                                                  gtgtAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCACTGAGTCACCATATTACGGGCGATGTTGGTTGCCACTTTAatat                                                                         >  1:173115/1‑242 (MQ=255)                                                                                                                                                                                  gtgtAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCACTGAGTCACCATATTACGGGCGATGTTGGTTGCCACTTTAATATCATTGGACg                                                                >  1:661188/1‑251 (MQ=255)                                                                                                                                                                                    gtAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCACTGAGTCACCATATTACGGGCGATGTTGGTTGCCACTTTCATATCATTGGACGc                                                               >  1:747376/1‑250 (MQ=255)                                                                                                                                                                                      aGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCACTGAGTCACCATATTAc                                                                                                    <  1:572269/211‑1 (MQ=255)                                                                                                                                                                                       gAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCACTGAGTCACCATATTACGGGCGATGTTGGTTGCCACTTTAATATCATTGGACGCCCCg                                                           >  1:474771/1‑251 (MQ=255)                                                                                                                                                                                         atatCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCACTGAGTCACCATATTACGGGCGATGTTGGTTGCCACTTTAATATCATTGGACGCCCCGGt                                                         >  1:548652/1‑251 (MQ=255)                                                                                                                                                                                           atCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCACTGAGTCACCATATTACGGGCGATGTTGGTTGCCACTTTAATATCATTGGACGCCCCGGTAg                                                       >  1:742960/1‑251 (MQ=255)                                                                                                                                                                                           atCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCACTGAGTCACCATATTACGGGCGATGTTGGTTGCCACTTTAATATCATTGGACGCCCCGGTAg                                                       <  1:185044/251‑1 (MQ=255)                                                                                                                                                                                             ccATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCACTg                                                                                                                  >  1:494167/1‑190 (MQ=255)                                                                                                                                                                                             ccATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCACTGAGTCACCATATTACGGGCGATGTTGGTTGCCACTTTAATATCATTGGACGCCCCGGTAg                                                       <  1:408653/249‑1 (MQ=255)                                                                                                                                                                                               atatTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCACTGAGTCACCATATTACGGGCGATGTTGGTTGcc                                                                                   <  1:191527/219‑1 (MQ=255)                                                                                                                                                                                                tattatCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCACTGAGTCACCATATTACGGGCGATGTTGGTTGCCACTTTAATATCATTGGACGCCCCGGTAGAAata                                                  <  1:806100/251‑1 (MQ=255)                                                                                                                                                                                                    atCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCACTGAGTCACCATATTAc                                                                                                    <  1:563810/197‑1 (MQ=255)                                                                                                                                                                                                        aTTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCACTGAGTCACCATATTACGGGCGATGTTGGTTGCCACTTTAATATCATTGGACGCCCCGGTAGAAATATTttctt                                           >  1:473347/1‑250 (MQ=255)                                                                                                                                                                                                                                        gTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGa                                                                                                                                                  >  1:675334/1‑115 (MQ=255)                                                                                                                                                                                                                                        gTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGa                                                                                                                                                  <  1:480159/115‑1 (MQ=255)                                                                                                                                                                                                                                        gTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCACTGAGTCACCATATTACGGGCGATGTTGGTTGCCACTTTAATATCATTGGAc                                                                 >  1:771124/1‑196 (MQ=255)                                                                                                                                                                                                                                        gTTCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCACTGAGTCACCATATTACGGGCGATGTTGGTTGCCACTTTAATATCATTGGACGCCCCGGTAGAAATATTTTCTTCACCGTAAATCAAATCTTCTGCCAAACGAc             >  1:248741/1‑248 (MQ=255)                                                                                                                                                                                                                                          tCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCACTGAGTCACCATATTAc                                                                                                    <  1:426707/159‑1 (MQ=255)                                                                                                                                                                                                                                          tCACAATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCACTGAGTCACCATATTACGGGCGATGTTGGTTGCCACTTTAATATCATTGGAc                                                                 >  1:637579/1‑194 (MQ=255)                                                                                                                                                                                                                                             caATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCACTGAGTCACCATATTACGGGCGATGTTGGTTGCCACTTTAATATCATTGGACGCCCCGGTAGAAATATTTTCTTCACCGTAAATCAAATCTTCTGCCAAACGa              <  1:788609/242‑1 (MQ=255)                                                                                                                                                                                                                                             caATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCACTGAGTCACCATATTACGGGCGATGTTGGTTGCCACTTTAATATCATTGGACGCCCCGGTAGAAATATTTTCTTCACCGTAAATCAAATCTTCTGCCAAACGa              <  1:84986/242‑1 (MQ=255)                                                                                                                                                                                                                                             caATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCACTGAGTCACCATATTACGGGCGATGTTGGTTGCCACTTTAATATCATTGGACGCCCCGGTAGAAATATTTTCTTCACCGTAAATCAAATCTTCTGCCAAACGACCTGCGtat     >  1:302301/1‑251 (MQ=255)                                                                                                                                                                                                                                             caATAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCACTGAGTCACCATATTACGGGCGATGTTGGTTGCCACTTTAATATCATTGGACGCCCCGGTAGAAATATTTTCTTCACCGTAAATCAAATCTTCTGCCAAACGACCTGCGtat     >  1:196864/1‑251 (MQ=255)                                                                                                                                                                                                                                                tAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCACTGAGTCACCATATTACGGGCGATGTTGGTTGCCACTTTAATATCATTGGACGCCCCGGTAGAAATATTTTCTTCACCGTAAATCAAATCTTCTGCCAAACGACCTGCGTATAAc  <  1:782771/251‑1 (MQ=255)                                                                                                                                                                                                                                                tAGCGCGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCACTGAGTCACCATATTACGGGCGATGTTGGTTGCCACTTTAATATCATTGGACGCCCCGGTAGAAATATTTTCTTCACCGTAAATCAAATCTTCTGCCAAACGACCTGCGTATAAc  <  1:468228/251‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                       GCCCTGCGCATCATCTGCCATTTTTGGCTCTTCAGCTTTCGGCTGCTGAGGTTCGGCTTTATCTGTATTCTTTGGTTCTTCCCAACCTGGAGGCGGTGTCACTGGTTGACGATTCATTAACTGCTTGATTTGTTCTTCTTCAATAGTTTCGTATTTCACCAGCGCATCTTTCATCGCGTGTAGAATATCCATATTATCGATTAAAATCTGTCTCGCGCGACCATAATTTCGGTTCACAATAGCACGAACTTCCTCATCGATCACATGTGCGGTTTCATCGGACATGTGTTTGGCTTTCGCCATGGAACGCCCTAAGAATACTTCCCCGTCGTCTTCGGCATAAAGAATTGGACCAAGTTTATCGGAGAAACCCCACTGAGTCACCATATTACGGGCGATGTTGGTTGCCACTTTAATATCATTGGACGCCCCGGTAGAAATATTTTCTTCACCGTAAATCAAATCTTCTGCCAAACGACCTGCGTATAAC  >  1/1195951‑1196440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑