Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,188,679	T→G	V474V (GTA→GTC)	unknown ←	Phosphoglycerate transport system sensor protein PgtB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,188,679	0	T	G	100.0%	108.9 / NA	41	V474V (GTA→GTC)	unknown	Phosphoglycerate transport system sensor protein PgtB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (23/18);  total (23/18)

TTCATCACCTAATACATCAGGTAAATCTGTAGGAAGAGATAACCGAGCATTCAGATATTTATGTTTTGGCTCAAGTAAATCCCACGCATTATTTATACACATCAATAAATTAATTTTCTGTAATGTATTTGTGGTTTGGCGGGAAAAATGGCGCAGTCTTTTCACCACCAGCGCCGTACGCTCCACTAAATTTTCTATTTTCTCTAAATAGTTATTCGCAGAAAAAACATCATTATTTTTTACCGCTCTTTTTGTACTGAAAATATACGCATTCATTGCATTTAGCGGTTGTGTGATTTCATGGCTAATAGATGTCAAAGTTTGTCCTACTACCGCTAATTTTGCCGCTTGAATTAGTTCTTGTTGTGTATTGATAAGTTTTTCCTGGATTGCAATACGTTCACTAATTTCATTTAATAAAATTTGATTTCTTCTTTCTAATTCTTCATTTTTTGTGTTAACTTCAAATAAAAATAA  >  1/1188439‑1188915                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                             ttCATCACCTAATACATCAGGTAAATCTGTAGGAAGAGATAACCGAGCATTCAGATATTTATGTTTTGGCTCAAGTAAATCCCACGCATTATTTATACACATCAATAAATTAATTTTCTGTAATGTATTTGTGGTTTGGCGGGAAAAATGGCGCAGTCTTTTCACCACCAGCGCCGTACGCTCCACTAAATTTTCTATTTTCTCTAAATAGTTATTCGCAGAAAAAACATCATTATTTTTGACCGCTCttt                                                                                                                                                                                                                                    >  1:493847/1‑251 (MQ=255)       aCCTAATACATCAGGTAAATCTGTAGGAAGAGATAACCGAGCATTCAGATATTTATGTTTTGGCTCAAGTAAATCCCACGCATTATTTATACACATCAATAAATTAATTTTCTGTAATGTATTTGTGGTTTGGCGGGAAAAATGGCGCAGTCTTTTCACCACCAGCGCCGTACGCTCCACTAAATTTTCTATTTTCTCTAAATAGTTATTCGCAGAAAAAACATCATTATTTTTGACCGCTCTTTTTGTa                                                                                                                                                                                                                               >  1:837058/1‑250 (MQ=255)              aCATCAGGTAAATCTGTAGGAAGAGATAACCGAGCATTCAGATATTTATGTTTTGGCTCAAGTAAATCCCACGCATTATTTATACACATCAATAAATTAATTTTCTGTAATGTATTTGTGGTTTGGCGGGAAAAATGGCGCAGTCTTTTCACCACCAGCGCCGTACGCTCCACTAAATTTTCTATTTTCTCTAAATAGTTATTCGCAGAAAAAACATCATTATTTTTGACCGCTCTTTTTGTACTGAAAat                                                                                                                                                                                                                       >  1:120674/1‑251 (MQ=255)               cATCAGGTAAATCTGTAGGAAGAGATAACCGAGCATTCAGATATTTATGTTTTGGCTCAAGTAAATCCCACGCATTATTTATACACATCAATAAATTAATTTTCTGTAATGTATTTGTGGTTTGGCGGGAAAAATGGCGCAGTCTTTTCACCACCAGCGCCGTACGCTCCACTAAATTTTCTATTTTCTCTAAATAGTTATTCGCAGAAAAAACATCATTATTTTTGACCGCTCTTTTTGTACTGAAAata                                                                                                                                                                                                                      >  1:399066/1‑251 (MQ=255)                           cTGTAGGAAGAGATAACCGAGCATTCAGATATTTATGTTTTGGCTCAAGTAAATCCCACGCATTATTTATACACATCAATAAATTAATTTTCTGTAATGTATTTGTGGTTTGGCGGGAAAAATGGCGCAGTCTTTTCACCACCAGCGCCGTACGCTCCACTAAATTTTCTATTTTCTCTAAATAGTTATTCGCAGAAAAAACATCATTATTTTTGACCGCTCTTTTTGTACTGAAAATATACGcattcatt                                                                                                                                                                                                          >  1:302348/1‑251 (MQ=255)                                     agaTAACCGAGCATTCAGATATTTATGTTTTGGCTCAAGTAAATCCCACGCATTATTTATACACATCAATAAATTAATTTTCTGTAATGTATTTGTGGTTTGGCGGGAAAAATGGCGCAGTCTTTTCACCACCAGCGCCGTACGCTCCACTAAATTTTCTATTTTCTCTAAATAGTTATTCGCAGAAAAAACATCATTATTTTTGACCGCTCTTTTTGTACTGAAAATATACGCATTCATTGCATTTAGc                                                                                                                                                                                                 <  1:215477/250‑1 (MQ=255)                                                       atatTTATGTTTTGGCTCAAGTAAATCCCACGCATTATTTATACACATCAATAAATTAATTTTCTGTAATGTATTTGTGGTTTGGCGGGAAAAATGGCGCAGTCTTTTCACCACCAGCGCCGTACGCTCCACTAAATTTTCTATTTTCTCTAAATAGTTATTCGCAGAAAAAACATCATTATTTTTGACCGCTCTTTTTGTACTGAAAATATACGCATTCATTGCATTTAGCGGt                                                                                                                                                                                              <  1:421308/235‑1 (MQ=255)                                                              tGTTTTGGCTCAAGTAAATCCCACGCATTATTTATACACATCAATAAATTAATTTTCTGTAATGTATTTGTGGTTTGGCGGGAAAAATGGCGCAGTCTTTTCACCACCAGCGCCGTACGCTCCACTAAATTTTCTATTTTCTCTAAATAGTTATTCGCAGAAAAAACATCATTATTTTTGACCGCTCTTTTTGTACTGAAAATATAc                                                                                                                                                                                                                   <  1:486084/207‑1 (MQ=255)                                                              tGTTTTGGCTCAAGTAAATCCCACGCATTATTTATACACATCAATAAATTAATTTTCTGTAATGTATTTGTGGTTTGGCGGGAAAAATGGCGCAGTCTTTTCACCACCAGCGCCGTACGCTCCACTAAATTTTCTATTTTCTCTAAATAGTTATTCGCAGAAAAAACATCATTATTTTTGACCGCTCTTTTTGTACTGAAAATATACGCATTCATTGCATTTAGCGGTTGTGTGATTTCATGGCTAATAGa                                                                                                                                                                       <  1:96752/251‑1 (MQ=255)                                                                    ggCTCAAGTAAATCCCACGCATTATTTATACACATCAATAAATTAATTTTCTGTAATGTATTTGTGGTTTGGCGGGAAAAATGGCGCAGTCTTTTCACCACCAGCGCCGTACGCTCCACTAAATTTTCTATTTTCTCTAAATAGTTATTCGCAGAAAAAACATCATTATTTTTGACCGCTCTTTTTGTACTGAAAATATACGCATTCATTGCATTTAGc                                                                                                                                                                                                 >  1:23493/1‑219 (MQ=255)                                                                                tCCCACGCATTATTTATACACATCAATAAATTAATTTTCTGTAATGTATTTGTGGTTTGGCGGGAAAAATGGCGCAGTCTTTTCACCACCAGCGCCGTACGCTCCACTAAATTTTCTATTTTCTCTAAATAGTTATTCGCAGAAAAAACATCATTATTTTTGACCGCTCTTTTTGTACTGAAAATATACGCATTCATTGCATTTAGCGGTTGTGTGATTTCATGGCTAATAGATGTCAAAGTTTGTCctac                                                                                                                                                     <  1:466673/251‑1 (MQ=255)                                                                                   cACGCATTATTTATACACATCAATAAATTAATTTTCTGTAATGTATTTGTGGTTTGGCGGGAAAAATGGCGCAGTCTTTTCACCACCAGCGCCGTACGCTCCACTAAATTTTCTATTTTCTCTAAATAGTTATTCGCAGAAAAAACATCATTATTTTTGACCGCTCTTTTTGTACTGAAAATATACGCATTCATTGCATTTAGCGGTTGTGTGATTTCATGGCTAATAGATGTCAAAGTTTGTCCtactac                                                                                                                                                  <  1:103545/251‑1 (MQ=255)                                                                                         ttatttatACACATCAATAAATTAATTTTCTGTAATGTATTTGTGGTTTGGCGGGAAAAATGGCGCAGTCTTTTCACCACCAGCGCCGTACGCTCCACTAAATTTTCTATTTTCTCTAAATAGTTATTCGCAGAAAAAACATCATTATTTTTGACCGCTCTTTTTGTACTGAAAATATACGCATTCATTGCATTTAGCGGt                                                                                                                                                                                              <  1:690520/201‑1 (MQ=255)                                                                                         ttatttatACACATCAATAAATTAATTTTCTGTAATGTATTTGTGGTTTGGCGGGAAAAATGGCGCAGTCTTTTCACCACCAGCGCCGTACGCTCCACTAAATTTTCTATTTTCTCTAAATAGTTATTCGCAGAAAAAACATCATTATTTTTGACCGCTCTTTTTGTACTGAAAATATACGCATTCATTGCATTTAGCGGTTGTGTGATTTCATGGCTAATAg                                                                                                                                                                        >  1:462403/1‑223 (MQ=255)                                                                                         ttatttatACACATCAATAAATTAATTTTCTGTAATGTATTTGTGGTTTGGCGGGAAAAATGGCGCAGTCTTTTCACCACCAGCGCCGTACGCTCCACTAAATTTTCTATTTTCTCTAAATAGTTATTCGCAGAAAAAACATCATTATTTTTGACCGCTCTTTTTGTACTGAAAATATACGCATTCATTGCATTTAGCGGTTGTGTGATTTCATGGCTAATAg                                                                                                                                                                        <  1:203486/223‑1 (MQ=255)                                                                                           atttatACACATCAATAAATTAATTTTCTGTAATGTATTTGTGGTTTGGCGGGAAAAATGGCGCAGTCTTTTCACCACCAGCGCCGTACGCTCCACTAAATTTTCTATTTTCTCTAAATAGTTATTCGCAGAAAAAACATCATTATTTTTGACCGCTCTTTTTGTACTGAAAATATACGCATTCATTg                                                                                                                                                                                                         >  1:625436/1‑188 (MQ=255)                                                                                               ataCACATCAATAAATTAATTTTCTGTAATGTATTTGTGGTTTGGCGGGAAAAATGGCGCAGTCTTTTCACCACCAGCGCCGTACGCTCCACTAAATTTTCTATTTTCTCTAAATAGTTATTCGCAGAAAAAACATCATTATTTTTGACCGCTCTTTTTGTACTGAAAATATACGCATTCATTGCATTTAGCgg                                                                                                                                                                                               >  1:809263/1‑194 (MQ=255)                                                                                                  cacaTCAATAAATTAATTTTCTGTAATGTATTTGAGGTTTGGCGGGAAAAATGGCGCAGTCTTTTCACCACCAGCGCCGTACGCTCCACTAAATTTTCTATTTTCTCTAAATAGTTATTCGCAGAAAAAACATCATTATTTTTGACCGCTCTTTTTGTACTGAAAATATACGCATTCATTGCATTTAGCGGTTGTGTGATTTCATGGCTAATAGATGTCAAAGTTTGTCTTACTACCGCTAATTTTGCCg                                                                                                                                    >  1:819164/1‑250 (MQ=255)                                                                                                                 attTTCTGTAATGTATTTGTGGTTTGGCGGGAAAAATGGCGCAGTCTTTTCACCACCAGCGCCGTACGCTCCACTAAATTTTCTATTTTCTCTAAATAGTTATTCGCAGAAAAAACATCATTATTTTTGACCGCTCTTTTTGTACTGAAAATATACGCATTCATTGCATTTAGCGGTTGTGTGATTTCATGGCTAATAGATGTCAAAGTTTGTCCTACTACCg                                                                                                                                                >  1:871415/1‑223 (MQ=255)                                                                                                                      cTGTAATGTATTTGTGGTTTGGCGGGAAAAATGGCGCAGTCTTTTCACCACCAGCGCCGTACGCTCCACTAAATTTTCTATTTTCTCTAAATAGTTATTCGCAGAAAAAACATCATTATTTTTGACCGCTCTTTTTGTACTGAAAATATACGCATTCATTGCATTTAGCGGTTGTGTGATTTCATGGCTAATAGATGTCAAAGTTTGTCCTACTACCGCTAATTTTGCCGCTTGAATTAGTTCTTGTtgtg                                                                                                               >  1:62720/1‑251 (MQ=255)                                                                                                                      cTGTAATGTATTTGTGGTTTGGCGGGAAAAATGGCGCAGTCTTTTCACCACCAGCGCCGTACGCTCCACTAAATTTTCTATTTTCTCTAAATAGTTATTCGCAGAAAAAACATCATTATTTTTGACCGCTCTTTTTGTACTGAAAATATACGCATTCATTGCATTTAGCGGTTGTGTGATTTCATGGCTAATAGATGTCAAAGTTTGTCCTACTACCGCTAATTTTGCCGCTTGAATTAGTTCTTGTtgtg                                                                                                               >  1:558678/1‑251 (MQ=255)                                                                                                                        gTAATGTATTTGTGGTTTGGCGGGAAAAATGGCGCAGTCTTTTCACCACCAGCGCCGTACGCTCCACTAAATTTTCTATTTTCTCTAAATAGTTATTCGCAGAAAAAACATCATTATTTTTGACCGCTCTTTTTGTACTGAAAATATACGCATTCATTGCAttt                                                                                                                                                                                                    >  1:204262/1‑164 (MQ=255)                                                                                                                              tATTTGTGGTTTGGCGGGAAAAATGGCGCAGTCTTTTCACCACCAGCGCCGTACGCTCCACTAAATTTTCTATTTTCTCTAAATAGTTATTCGCAGAAAAAACATCATTATTTTTGACCGCTCTTTTTGTACTGAAAATATACGCATTCATTGCATTTAGCGGTTGTGTGATTTCATGGCTAATAGATGTCAAAGTTTGTCCTACTACCGCTAATTTTGCCGCTTGAATTAGTTCTTGTtgtg                                                                                                               >  1:413371/1‑243 (MQ=255)                                                                                                                              tATTTGTGGTTTGGCGGGAAAAATGGCGCAGTCTTTTCACCACCAGCGCCGTACGCTCCACTAAATTTTCTATTTTCTCTAAATAGTTATTCGCAGAAAAAACATCATTATTTTTGACCGCTCTTTTTGTACTGAAAATATACGCATTCATTGCATTTAGCGGTTGTGTGATTTCATGGCTAATAGATGTCAAAGTTTGTCCTACTACCGCTAATTTTGCCGCTTGAATTAGTTCTTGTTGTGTATTGATa                                                                                                       <  1:511550/251‑1 (MQ=255)                                                                                                                                        ttGGCGGGAAAAATGGCGCAGTCTTTTCACCACCAGCGCCGTACGCTCCACTAAATTTTCTATTTTCTCTAAATAGTTATTCGCAGAAAAAACATCATTATTTTTGACCGCTCTTTTTGTACTGAAAATATACGCATTCATTGCATTTAGCGGTTGTGTGATTTCATGGCTAATAGATGTCAAAGTTTGTCCTACTACCGCTAATTTTGCCGCTTGAATTAGTTCTTGTTGTGTATTGATAAGTTTTTCCt                                                                                             <  1:374778/251‑1 (MQ=255)                                                                                                                                                            gTCTTTTCACCACCAGCGCCGTACGCTCCACTAAATTTTCTATTTTCTCTAAATAGTTATTCGCAGAAAAAACATCATTATTTTTGACCGCTCTTTTTGTACTGAAAATATACGCATTCATTGCATTTAGCGGTTGTGTGATTTCATGGCTAATAg                                                                                                                                                                        >  1:290728/1‑156 (MQ=255)                                                                                                                                                            gTCTTTTCACCACCAGCGCCGTACGCTCCACTAAATTTTCTATTTTCTCTAAATAGTTATTCGCAGAAAAAACATCATTATTTTTGACCGCTCTTTTTGTACTGAAAATATACGCATTCATTGCATTTAGCGGTTGTGTGATTTCATGGCTAATAGATGTCAAAGTTTGTCctacta                                                                                                                                                   <  1:119721/177‑1 (MQ=255)                                                                                                                                                            gTCTTTTCACCACCAGCGCCGTACGCTCCACTAAATTTTCTATTTTCTCTAAATAGTTATTCGCAGAAAAAACATCATTATTTTTGACCGCTCTTTTTGTACTGAAAATATACGCATTCATTGCATTTAGCGGTTGTGTGATTTCATGGCTAATAGATGTCAAAGTTTGTCCTACTACCGCTAATTTTGCCGCTTGAATTAGTTCttgt                                                                                                                   >  1:288265/1‑209 (MQ=255)                                                                                                                                                            gTCTTTTCACCACCAGCGCCGTACGCTCCACTAAATTTTCTATTTTCTCTAAATAGTTATTCGCAGAAAAAACATCATTATTTTTGACCGCTCTTTTTGTACTGAAAATATACGCATTCATTGCATTTAGCGGTTGTGTGATTTCATGGCTAATAGATGTCAAAGTTTGTCCTACTACCGCTAATTTTGCCGCTTGAATTAGTTCTTGTTGTGTATTGATAAGTTTTTCCTGGATTGCAATACGTTCACTa                                                                         >  1:72695/1‑251 (MQ=255)                                                                                                                                                                tttCACCACCAGCGCCGTACGCTCCACTAAATTTTCTATTTTCTCTAAATAGTTATTCGCAGAAAAAACATCATTATTTTTGACCGCTCTTTTTGTACTGAAAATATACGCATTCATTGCATTTAGCGGTTGTGTGATTTCATGGCTAATAGATGTCAAAGTTTGTCCTACTACCGCTAATTTTGCCGCTTGAATTAGTTCttgttg                                                                                                                 >  1:537926/1‑207 (MQ=255)                                                                                                                                                                tttCACCACCAGCGCCGTACGCTCCACTAAATTTTCTATTTTCTCTAAATAGTTATTCGCAGAAAAAACATCATTATTTTTGACCGCTCTTTTTGTACTGAAAATATACGCATTCATTGCATTTAGCGGTTGTGTGATTTCATGGCTAATAGATGTCAAAGTTTGTCCTACTACCGCTAATTTTGCCGCTTGAATTAGTTCTTGTTGTGTATTGATa                                                                                                       <  1:23530/217‑1 (MQ=255)                                                                                                                                                                     ccaccaGCGCCGTACGCTCCACTAAATTTTCTATTTTCTCTAAATAGTTATTCGCAGAAAAAACATCATTATTTTTGACCGCTCTTTTTGTACTGAAAATATACGCATTCATTGCATTTAGCGGTTGTGTGATTTCATGGCTAATAGATGTCAAAGTTTGTCCTACTACCGCTAATTTTGCCGCTTGAATTAGTTCTTGTTGTGTATTGATAAGTTTTTCCTGGATTGCAATACGTTCACTAATTTCAttt                                                                >  1:371518/1‑251 (MQ=255)                                                                                                                                                                                                tttctattttctCTAAATAGTTATTCGCAGAAAAAACATCATTATTTTTGACCGCTCTTTTTGTACTGAAAATATACGCATTCATTGCATTTAGCGGTTGTGTGATTTCATGGCTAATAg                                                                                                                                                                        >  1:6975/1‑120 (MQ=255)                                                                                                                                                                                                  tctattttctCTAAATAGTTATTCGCAGAAAAAACATCATTATTTTTGACCGCTCTTTTTGTACTGAAAATATACGCATTCATTGCATTTAGCGGTTGTGTGATTTCATGGCTAATAg                                                                                                                                                                        <  1:15094/118‑1 (MQ=255)                                                                                                                                                                                                         tctcTAAATAGTTATTCGCAGAAAAAACATCATTATTTTTGACCGCTCTTTTTGTACTGAAAAtata                                                                                                                                                                                                                    <  1:472428/67‑1 (MQ=255)                                                                                                                                                                                                              aaaTAGTTATTCGCAGAAAAAACATCATTATTTTTGACCGCTCTTTTTGTACTGAAAATATACGCATTCATTGCATTTAGCGGTTGTGTGATTTCATGGCTAATAGATGTCAAAGTTTGTCCTACTACCGCTAATTTTGCCGCTTGAATTAGTTCTTGTTGTGTATTGATAAGTTTTTCCTGGATTGCAATACGTTCACTAATTTCATTtaataa                                                           <  1:111859/215‑1 (MQ=255)                                                                                                                                                                                                                                   aCATCATTATTTTTGACCGCTCTTTTTGTACTGAAAATATACGCATTCATTGCATTTAGCGGTTGTGTGATTTCATGGCTAATAGATGTCAAAGTTTGTCCTACTACCGCTAATTTTGCCGCTTGAATTAGTTCTTGTTGTGTATTGATAAGTTTTTCCTGGATTGCAAt                                                                                   <  1:256697/170‑1 (MQ=255)                                                                                                                                                                                                                                   aCATCATTATTTTTGACCGCTCTTTTTGTACTGAAAATATACGCATTCATTGCATTTAGCGGTTGTGTGATTTCATGGCTAATAGATGTCAAAGTTTGTCCTACTACCGCTAATTTTGCCGCTTGAATTAGTTCTTGTTGTGTATTGATAAGTTTTTCCTGGATTGCAATACGTTCACTAATTTCATTTAATAAAATTTGATTTCTTCTTTCTAATTCTTCATTTTTTGTGTTAACTTCaaataaaaataa  >  1:778263/1‑251 (MQ=255)                                                                                                                                                                                                                                      tcatTATTTTTGACCGCTCTTTTTGTACTGAAAATATACGCATTCATTGCATTTAGCGGTTGTGTGATTTCATGGCTAATAGATGTCAAAGTTTGTCCTACTACCGCTAATTTTGCCGCTTGAATTAGTTCTTGTTGTGTATTGATAAGTTTTTcc                                                                                              >  1:524935/1‑156 (MQ=255)                                                                                                                                                                                                                                      tcatTATTTTTGACCGCTCTTTTTGTACTGAAAATATACGCATTCATTGCATTTAGCGGTTGTGTGATTTCATGGCTAATAGATGTCAAAGTTTGTCCTACTACCGCTAATTTTGCCGCTTGAATTAGTTCTTGTTGTGTATTGATAAGTTTTTCCTGGATTGCAATACGTTCACTAATTTCAtt                                                                 <  1:161691/185‑1 (MQ=255)                                                                                                                                                                                                                                           attTTTGACCGCTCTTTTTGTACTGAAAATATACGCATTCATTGCATTTAGCGGTTGTGTGATTTCATGGCTAATAGATGTCAAAGTTTGTCCTACTACCGCTAATTTTGCCGCTTGAATTAGTTCTTGTTGTGTATTGATAAGTTTTTCCTGGATTGCAATACGTTCACTAATTTCATTTAATAAAATTTGATTTCTTCTTTCTaa                                      <  1:485612/207‑1 (MQ=255)                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                             TTCATCACCTAATACATCAGGTAAATCTGTAGGAAGAGATAACCGAGCATTCAGATATTTATGTTTTGGCTCAAGTAAATCCCACGCATTATTTATACACATCAATAAATTAATTTTCTGTAATGTATTTGTGGTTTGGCGGGAAAAATGGCGCAGTCTTTTCACCACCAGCGCCGTACGCTCCACTAAATTTTCTATTTTCTCTAAATAGTTATTCGCAGAAAAAACATCATTATTTTTTACCGCTCTTTTTGTACTGAAAATATACGCATTCATTGCATTTAGCGGTTGTGTGATTTCATGGCTAATAGATGTCAAAGTTTGTCCTACTACCGCTAATTTTGCCGCTTGAATTAGTTCTTGTTGTGTATTGATAAGTTTTTCCTGGATTGCAATACGTTCACTAATTTCATTTAATAAAATTTGATTTCTTCTTTCTAATTCTTCATTTTTTGTGTTAACTTCAAATAAAAATAA  >  1/1188439‑1188915

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑