Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,181,617	T→G	S392R (AGC→CGC)	unknown ←	Lon protease homolog YcbZ

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,181,617	0	T	G	100.0%	203.2 / NA	73	S392R (AGC→CGC)	unknown	Lon protease homolog YcbZ
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (33/40);  total (33/40)

GAATTGGTCAATAGCGCCCGTAATGGCGATAGATTGCGGCAGAGGCAAAGCGGACAATGCGCTCAATAATACACAAAAACCGGCGAGTGAGGCACTGTCACCGTCAATTTCACCGTAAGATTGCTCAAACACCAAAGAAGCGGAAAACGGTAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCTTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCGGATATTCAATCACCGATAAGCCGTTAATTTGCCCAACCATTTCACCTTCGGTCGCTACATAAATTTGTTCCTGCAAAATAGCGTCATAGGTTTGTTCACGCAAAAAGCCATATTGTGCCGTTTTATGTAAAAAATACCGTTCAAAATCCACCGCACTTAGTG  >  1/1181375‑1181862                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      gAATTGGTCAATAGCGCCCGTAATGGCGATAGATTGCGGCAGAGGCAAAGCGGACAATGCGCTCAATAATACACAAAAACCGGCGAGTGAGGCACTGTCACCGTCAATTTCACCGTAAGATTGCTCAAACACCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACAt                                                                                                                                                                                                                                               >  1:791582/1‑251 (MQ=255)       gTCAATAGCGCCCGTAATGGCGATAGATTGCGGCAGAGGCAAAGCGGACAATGCGCTCAATAATACACAAAAACCGGCGAGTGAGGCACTGTCACCGTCAATTTCACCGTAAGATTGCTCAAACACCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATcgacga                                                                                                                                                                                                                                         >  1:132358/1‑251 (MQ=255)                               aGATTGCGGCAGAGGCAAAGCGGACAATGCGCTCAATAATACACAAAAACCGGCGAGTGAGGCACTGTCACCGTCAATTTCACCGTAAGATTGCTCAAACACCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCGTAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACaa                                                                                                                                                                                                                 >  1:434437/1‑251 (MQ=255)                                  ttGCGGCAGAGGCAAAGCGGACAATGCGCTCAATAATACACAAAAACCGGCGAGTGAGGCACTGTCACCGTCAATTTCACCGTAAGATTGCTCAAACACCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATAc                                                                                                                                                                                                              <  1:12065/251‑1 (MQ=255)                                  ttGCGGCAGAGGCAAAGCGGACAATGCGCTCAATAATACACAAAAACCGGCGAGTGAGGCACTGTCACCGTCAATTTCACCGTAAGATTGCTCAAACACCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATAc                                                                                                                                                                                                              <  1:348751/251‑1 (MQ=255)                                  ttGCGGCAGAGGCAAAGCGGACAATGCGCTCAATAATACACAAAAACCGGCGAGTGAGGCACTGTCACCGTCAATTTCACCGTAAGATTGCTCAAACACCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATAc                                                                                                                                                                                                              <  1:714664/251‑1 (MQ=255)                                       gCAGAGGCAAAGCGGACAATGCGCTCAATAATACACAAAAACCGGCGAGTGAGGCACTGTCACCGTCAATTTCACCGTAAGATTGCTCAAACACCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACt                                                                                                                                                                                                          <  1:847331/250‑1 (MQ=255)                                        cAGAGGCAAAGCGGACAATGCGCTCAATAATACACAAAAACCGGCGAGTGAGGCACTGTCACCGTCAATTTCACCGTAAGATTGCTCAAACACCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTg                                                                                                                                                                                                         <  1:36361/250‑1 (MQ=255)                                             gCAAAGCGGACAATGCGCTCAATAATACACAAAAACCGGCGAGTGAGGCACTGTCACCGTCAATTTCACCGTAAGATTGCTCAAACACCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTc                                                                                                                                                                                                     <  1:821124/249‑1 (MQ=255)                                             gCAAAGCGGACAATGCGCTCAATAATACACAAAAACCGGCGAGTGAGGCACTGTCACCGTCAATTTCACCGTAAGATTGCTCAAACACCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTc                                                                                                                                                                                                     <  1:4770/249‑1 (MQ=255)                                                  gCGGACAATGCGCTCAATAATACACAAAAACCGGCGAGTGAGGCACTGTCACCGTCAATTTCACCGTAAGATTGCTCAAACACCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAAc                                                                                                                                                                                                           >  1:15971/1‑238 (MQ=255)                                                           gcgcTCAATAATACACAAAAACCGGCGAGTGAGGCACTGTCACCGTCAATTTCACCGTAAGATTGCTCAAACACCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTGCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGaa                                                                                                                                                                                     >  1:341070/1‑251 (MQ=255)                                                                cAATAATACACAAAAACCGGCGAGTGAGGCACTGTCACCGTCAATTTCACCGTAAGATTGCTCAAACACCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTg                                                                                                                                                                                                         <  1:889226/226‑1 (MQ=255)                                                                cAATAATACACAAAAACCGGCGAGTGAGGCACTGTCACCGTCAATTTCACCGTAAGATTGCTCAAACACCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTg                                                                                                                                                                                                         >  1:779445/1‑226 (MQ=255)                                                                cAATAATACACAAAAACCGGCGAGTGAGGCACTGTCACCGTCAATTTCACCGTAAGATTGCTCAAACACCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGa                                                                                                                                                                                      >  1:725661/1‑245 (MQ=255)                                                                  ataataCACAAAAACCGGCGAGTGAGGCACTGTCACCGTCAATTTCACCGTAAGATTGCTCAAACACCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTg                                                                                                                                                                                                         >  1:793012/1‑224 (MQ=255)                                                                    aataCACAAAAACCGGCGAGTGAGGCACTGTCACCGTCAATTTCACCGTAAGATTGCTCAAACACCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAgg                                                                                                                                                                            <  1:499807/251‑1 (MQ=255)                                                                       acacAAAAACCGGCGAGTGAGGCACTGTCACCGTCAATTTCACCGTAAGATTGCTCAAACACCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGAt                                                                                                                                                                                 <  1:513869/243‑1 (MQ=255)                                                                       acacAAAAACCGGCGAGTGAGGCACTGTCACCGTCAATTTCACCGTAAGATTGCTCAAACACCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGt                                                                                                                                                                         >  1:642463/1‑251 (MQ=255)                                                                        cacaAAAACCGGCGAGTGAGGCACTGTCACCGTCAATTTCACCGTAAGATTGCTCAAACACCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAAc                                                                                                                                                                                                           >  1:624720/1‑216 (MQ=255)                                                                        cacaAAAACCGGCGAGTGAGGCACTGTCACCGTCAATTTCACCGTAAGATTGCTCAAACACCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAgg                                                                                                                                                                            >  1:543048/1‑247 (MQ=255)                                                                             aaaCCGGCGAGTGAGGCACTGTCACCGTCAATTTCACCGTAAGATTGCTCAAACACCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCgg                                                                                                                                                                   >  1:106146/1‑251 (MQ=255)                                                                                     gAGTGAGGCACTGTCACCGTCAATTTCACCGTAAGATTGCTCAAACACCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAgg                                                                                                                                                                            >  1:9082/1‑234 (MQ=255)                                                                                       gTGAGGCACTGTCACCGTCAATTTCACCGTAAGATTGCTCAAACACCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAAc                                                                                                                                                                                                           >  1:827613/1‑201 (MQ=255)                                                                                        tGAGGCACTGTCACCGTCAATTTCACCGTAAGATTGCTCAAACACCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCGGATATTCAATc                                                                                                                                                         <  1:700044/250‑1 (MQ=255)                                                                                         gAGGCACTGTCACCGTCAATTTCACCGTAAGATTGCTCAAACACCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTg                                                                                                                                                                                                         >  1:880737/1‑201 (MQ=255)                                                                                          aGGCACTGTCACCGTCAATTTCACCGTAAGATTGCTCAAACACCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCGGATATTCAATCAc                                                                                                                                                       <  1:754104/250‑1 (MQ=255)                                                                                           ggCACTGTCACCGTCAATTTCACCGTAAGATTGCTCAAACACCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAAc                                                                                                                                                                                                           <  1:885500/197‑1 (MQ=255)                                                                                             cACTGTCACCGTCAATTTCACCGTAAGATTGCTCAAACACCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAgg                                                                                                                                                                            <  1:362767/226‑1 (MQ=255)                                                                                               cTGTCACCGTCAATTTCACCGTAAGATTGCTCAAACACCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGc                                                                                                                                                                           >  1:230912/1‑225 (MQ=255)                                                                                                  tCACCGTCAATTTCACCGTAAGATTGCTCAACCACCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCGGATATTCAATCACCGATAAGc                                                                                                                                               <  1:797014/250‑1 (MQ=255)                                                                                                         cAATTTCACCGTAAGATTGCTCAAACACCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCGGATATTCAATCACCGATAAGCCGTTAAtt                                                                                                                                       >  1:583843/1‑251 (MQ=255)                                                                                                          aaTTTCACCGTAAGATTGCTCAAACACCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAgg                                                                                                                                                                            <  1:432963/213‑1 (MQ=255)                                                                                                                 ccGTAAGATTGCTCAAACACCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAgg                                                                                                                                                                                               <  1:23006/187‑1 (MQ=255)                                                                                                                 ccGTAAGATTGCTCAAACACCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCGGATATTCAATCACCGATAAGCCGTTAATTTGCCCaa                                                                                                                                >  1:434003/1‑250 (MQ=255)                                                                                                                 ccGTAAGATTGCTCAAACACCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCGGATATTCAATCACCGATAAGCCGTTAATTTGCCCaa                                                                                                                                >  1:450650/1‑250 (MQ=255)                                                                                                                 ccGTAAGATTGCTCAAACACCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCGGATATTCAATCACCGATAAGCCGTTAATTTGCCCaa                                                                                                                                >  1:773429/1‑250 (MQ=255)                                                                                                                         ttGCTCAAACACCAAAGAAGCGAAAAACGGCAGTTGTGACGGAAATTCGAGAAGATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCGGATATTCAATCACCGATAAGCCGTTAATTTGCCCAACCATTTCAc                                                                                                                       <  1:467663/251‑1 (MQ=255)                                                                                                                          tGCTCAAACACCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCGGATATTCAATCACCGATAAGCCGTTAATTTGCCCAACCATTTCAcc                                                                                                                      <  1:297147/251‑1 (MQ=255)                                                                                                                          tGCTCAAACACCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCGGATATTCAATCACCGATAAGCCGTTAATTTGCCCAACCATTTCAcc                                                                                                                      <  1:407777/251‑1 (MQ=255)                                                                                                                           gCTCAAACACCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCGGATATTCAATCACCGATAAGCCGTTAATTTGCCCAACCATTTCAc                                                                                                                       >  1:415788/1‑249 (MQ=255)                                                                                                                            cTCAAACACCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCGGATATTCAATCACCGATAAGCCGTTAATTTGCCCAACCATTTCACCtt                                                                                                                    <  1:152197/251‑1 (MQ=255)                                                                                                                                 acacCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCGGATATTCAATCACCGATAAGCCGTTAATTTGCCCAACCATTTCACCTTCGGTc                                                                                                               <  1:602211/251‑1 (MQ=255)                                                                                                                                 acacCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCGGATATTCAATCACCGATAAGCCGTTAATTTGCCCAACCATTTCACCTTCGGTc                                                                                                               <  1:596460/251‑1 (MQ=255)                                                                                                                                  cacCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATAc                                                                                                                                                                                                              <  1:164646/155‑1 (MQ=255)                                                                                                                                  cacCAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCGGATATTCAATCACCGATAAGc                                                                                                                                               >  1:399734/1‑218 (MQ=255)                                                                                                                                    ccAAAGAAGCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCGGATATTCAATCACCGATAAGCCGTTAATTTGCCCAACCATTTCACCTTCGGTCg                                                                                                              >  1:199213/1‑249 (MQ=255)                                                                                                                                         gaagCGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCGGATATTCAATCACCGATAAGCCGTTAATTTGCCCAACCATTTCACCTTCGGTCGCTACATa                                                                                                       >  1:448761/1‑251 (MQ=255)                                                                                                                                             cGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCGGATATTCAATCACCGATAAGCCGTTAATTTGCCCAACCATTTCACCTTCGGTCGCTACATAAAtt                                                                                                   <  1:582314/251‑1 (MQ=255)                                                                                                                                             cGGAAAACGGCAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCGGATATTCAATCACCGATAAGCCGTTAATTTGCCCAACCATTTCACCTTCGGTCGCTACATAAAtt                                                                                                   <  1:695512/251‑1 (MQ=255)                                                                                                                                                             tgACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCGGATATTCAATCACCGATAAGCCGTTAATTTGCCCAACCATTTCACCTTCGGTCGCTACATAAATTTGTTCCTGCAAAATa                                                                                    <  1:604200/250‑1 (MQ=255)                                                                                                                                                                 ggAAATTCGAGAATATTCGCAAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCGGATATTCAATCACCGATAAGCCGTTAATTTGCCCAACCATTTCACCtt                                                                                                                    <  1:840382/214‑1 (MQ=255)                                                                                                                                                                              tatTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCGGATATTCAATCACCGATAAGCCGTTAATTTGCCCAACCATTTCACCTTCGGTCGCTACATAAATTTGTTCCTGCAAAATAGCGTCATAGGTTTGTTCa                                                                  <  1:541362/251‑1 (MQ=255)                                                                                                                                                                                      aaaCAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTTCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCGGATATTCAATCACCGATAAGCCGTTAATTTGCCCAACCATTTCACCTTCGGTCGCTACATAAATTTGTTCCTGCAAAATAGCGTCATAGGTTTGTTCACGCaaaaa                                                          >  1:300015/1‑251 (MQ=255)                                                                                                                                                                                      aaaCAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCGGATATTCAATCACCGATAAGCCGTTAATTTGCCCAACCATTTCACCTTCGGTCGCTACATAAAtt                                                                                                   <  1:646019/210‑1 (MQ=255)                                                                                                                                                                                           ggCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCGGATATTCAATCACCGATAAGCCGTTAATTTGCCCAACCATTTCACCTTCGGTCGCTACATAAATTTGTTCCTGCAAAATAGCGTCATAGGTTTGTTCACGCAAAAAGCCat                                                     >  1:508504/1‑251 (MQ=255)                                                                                                                                                                                             cTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCGCATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCTGATATTCAATCACCGATAAGCCGTTAATTTGCCCAACCATTTCACCTTCGGTCGCTACATAAATTTGTTCCTGCAAAATAGCGTCATAGGTTTGTTCACGCAAAAAGCCatat                                                   <  1:191007/251‑1 (MQ=255)                                                                                                                                                                                             cTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCGGATATTCAATCACCGATAAGCCGTTAATTTGCCCAACCATTTCACCTTCGGTCGCTACATAAATTTGTTCCTGCAAAATAGCGTCATAGGTTTGTTCACGCAAAAAGCCatat                                                   <  1:117714/251‑1 (MQ=255)                                                                                                                                                                                             cTTCGGAAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCGGATATTCAATCACCGATAAGCCGTTAATTTGCCCAACCATTTCACCTTCGGTCGCTACATAAATTTGTTCCTGCAAAATAGCGTCATAGGTTTGTTCACGCAAAAAGCCatat                                                   <  1:558741/251‑1 (MQ=255)                                                                                                                                                                                               tCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCTGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCGGATATTCAATCACCGATAAGCCGTTAATTTGCCCAACCATTTCACCTTCGGTCGCTACATAAATTTGTTCCTGCAAAATAGCGTCATAGGTTTGTTCACGCAAAAAGCCATATtg                                                 <  1:402297/251‑1 (MQ=255)                                                                                                                                                                                                     aTCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGcc                                                                                                                                                                      <  1:19028/128‑1 (MQ=255)                                                                                                                                                                                                      tCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCGGATATTCAATCACCGATAAGCCGTTAATTTGCCCAACCATTTCACCTTCGGTCGCTACATAAAtt                                                                                                   >  1:843585/1‑194 (MQ=255)                                                                                                                                                                                                      tCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCGGATATTCAATCACCGATAAGCCGTTAATTTGCCCAACCATTTCACCTTCGGTCGCTACATAAATTTGTTCCTGCAAAATAGCGTCATAGGTTTGTTc                                                                   >  1:561135/1‑226 (MQ=255)                                                                                                                                                                                                      tCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCGGATATTCAATCACCGATAAGCCGTTAATTTGCCCAACCATTTCACCTTCGGTCGCTACATAAATTTGTTCCTGCAAAATAGCGTCATAGGTTTGTTCACGCAAAAAGCCatat                                                   >  1:437071/1‑242 (MQ=255)                                                                                                                                                                                                             gCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGGACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCGGATATTCAATCACCGATAAGCCGTTAATTTGCCCAACCATTTCACCTTCGGTCGCTACATAAATTTGTTCCTGCAAAAt                                                                                     <  1:327161/201‑1 (MQ=255)                                                                                                                                                                                                              ccTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGCTCTCCGAAAGATAAAGGCGTGCCCGGATATTCAATCACCGATAAGCCGTTAATTTGCCCAACCATTTCACCTTCGGTCGCTACATAAATTTGTTCCTGCAAAATAGCGTCATAGGTTTGTTCACGCAAAAAGCCATATTGTGCCGTTTTATGTaa                                  >  1:422711/1‑251 (MQ=255)                                                                                                                                                                                                                 ttGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCGGATATTCAATCACCGATAAGCCGTTAATTTGCCCAACCATTTCACCTTCGGTCGCTACATAAATTTGTTCCTGCAAAATAGCGTCATAGGTTTGTTCACGCAAAAAGCCATATTGTGCCGTTTTATGTaaaaa                               <  1:660055/251‑1 (MQ=255)                                                                                                                                                                                                                   gCTGTGAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCGGATATTCAATCACCGATAAGCCGTTAATTTGCCCAACCATTTCACCTTCGGTCGCTACATAAATTTGTTCCTGCAAAATAGCGTCATAGGTTTGTTCACGCAAAAAGCCATATTGTGc                                              >  1:292185/1‑234 (MQ=255)                                                                                                                                                                                                                     tgtgAATGTTGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCGGATATTCAATCACCGATAAGCCGTTAATTTGCCCAACCATTTCACCTTCGGTCGCTACATAAATTTGTTCCTGCAAAATAGCGTCATAGGTTTGTTCACGCAAAAAGCCATATTGTGCCGTTTTATGTAAAAAATa                            >  1:838891/1‑250 (MQ=255)                                                                                                                                                                                                                             ttGCCCGCCAGTTCATTTTTGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCGGATATTCAATCACCGATAAGCCGTTAATTTGCCCAACCa                                                                                                                             >  1:12448/1‑145 (MQ=255)                                                                                                                                                                                                                                              tttGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCGGATATTCAATCACCGATAAGCCGTTAATTTGCCCAACCATTTCACCTTCGGTCGCTACATAAATTTGTTCCTGCAAAATAGCGTCATAGGTTTGTTCACGCAAAAAGCCATATTGTGCCGTTTTATGTAAAAAATACCGTTCAAAATCCACCGCACTTAGTg  <  1:259542/251‑1 (MQ=255)                                                                                                                                                                                                                                              tttGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCGGATATTCAATCACCGATAAGCCGTTAATTTGCCCAACCATTTCACCTTCGGTCGCTACATAAATTTGTTCCTGCAAAATAGCGTCATAGGTTTGTTCACGCAAAAAGCCATATTGTGCCGTTTTATGTAAAAAATACCGTTCAAAATCCACCGCACTTAGTg  <  1:258115/251‑1 (MQ=255)                                                                                                                                                                                                                                              tttGCGTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCGGATATTCAATCACCGATAAGCCGTTAATTTGCCCAACCATTTCACCTTCGGTCGCTACATAAATTTGTTCCTGCAAAATAGCGTCATAGGTTTGTTCACGCAAAAAGCCATATTGTGCCGTTTTATGTAAAAAATACCGTTCAAAATCCACCGCACTTAGTg  <  1:347552/251‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                      GAATTGGTCAATAGCGCCCGTAATGGCGATAGATTGCGGCAGAGGCAAAGCGGACAATGCGCTCAATAATACACAAAAACCGGCGAGTGAGGCACTGTCACCGTCAATTTCACCGTAAGATTGCTCAAACACCAAAGAAGCGGAAAACGGTAGTTGTGACGGAAATTCGAGAATATTCGCCAAACAGGCTTCGGCAATCATAATGCCTTTGCTGTGAATGTTGCCCGCCAGTTCATTTTTGCTTTCCACATCGACGATTTCGCCGTCGCCGAATTGCACAATACAACTGATTCGAGAGGGTTCTCCGAAAGATAAAGGCGTGCCCGGATATTCAATCACCGATAAGCCGTTAATTTGCCCAACCATTTCACCTTCGGTCGCTACATAAATTTGTTCCTGCAAAATAGCGTCATAGGTTTGTTCACGCAAAAAGCCATATTGTGCCGTTTTATGTAAAAAATACCGTTCAAAATCCACCGCACTTAGTG  >  1/1181375‑1181862

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑