Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,159,946	T→C	A147A (GCT→GCC)	unknown →	FIG002813: LPPG:FO 2‑phospho‑L‑lactate transferase like, CofD‑like

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,159,946	0	T	C	100.0%	155.1 / NA	55	A147A (GCT→GCC)	unknown	FIG002813: LPPG:FO 2‑phospho‑L‑lactate transferase like, CofD‑like
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (29/26);  total (29/26)

ATTGTTTAAATCAAATCATTACGCAGCCGAGCACTGCCTCCGCCTTATTTGAATATCGCTTCAGCGGCAACGGCGAACTGGCGGGACACAATCTCGGCAATCTGATGCTAAAAGCATTGGAAGATTTACATATTCGCCCGATGGAAGCGGTTAATCTGATTCGTGATTTGCTTGATGTCAAATCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCTATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAACCGAACTACCAAAATCCATTTTTCTTGTTCCCTATGTGCAAGCCACGCCGGAAGCCATTAGCGCATTAAAAGAAGCTGAAGTGATTTTATTCGGTCCCGGCAGTTTTTTAACCAGCATTCTACCGCCGATTCTCTTGTCTGAAGTCATTGAACAACTGCAACAAAGCCAAGCCAAGAAAAT  >  1/1159720‑1160179                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                          aTTGTTTAAATCAAATCATTACGCAGCCGAGCACTGCCTCCGCCTTATTTGAATATCGCTTCAGCGGCAACGGCGAACTGGCGGGACACAATCTCGGCAATCTGATGCTAAAAGCATTGGAAGATTTACATATTCGCCCGATGGAAGCGGTTAATCTGATTCGTGATTTGCTTGATGTCAAATCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTg                                                                                                                                                                                                                   >  1:48076/1‑251 (MQ=255)                   ttACGCAGCCGAGCACTGCCTCCGCCTTATTTGAATATCGCTTCAGCGGCAACGGCGAACTGGCGGGACACAATCTCGGCAATCTGATGCTAAAAGCATTGGAAGATTTACATATTCGCCCGATGGAAGCGGTTAATCTGATTCGTGATTTGCTTGATGTCAAATCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCCCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGCAGGCGAAGCGAGCGTg                                                                                                                                                                                                 >  1:226666/1‑251 (MQ=255)                     aCGCAGCCGAGCACTGCCTCCGCCTTATTTGAATATCGCTTCAGCGGCAACGGCGAACTGGCGGGACACAATCTCGGCAATCTGATGCTAAAAGCATTGGAAGATTTACATATTCGCCCGATGGAAGCGGTTAATCTGATTCGTGATTTGCTTGATGTCAAATCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGa                                                                                                                                                                                               <  1:779721/251‑1 (MQ=255)                      cGCAGCCGAGCACTGCCTCCGCCTTATTTGAATATCGCTTCAGCGGCAACGGCGAACTGGCGGGACACAATCTCGGCAATCTGATGCTAAAAGCATTGGAAGATTTACATATTCGCCCGATGGAAGCGGTTAATCTGATTCGTGATTTGCTTGATGTCAAATCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGAc                                                                                                                                                                                              <  1:413388/251‑1 (MQ=255)                          gCCGAGCACTGCCTCCGCCTTATTTGAATATCGCTTCAGCGGCAACGGCGAACTGGCGGGACACAATCTCGGCAATCTGATGCTAAAAGCATTGGAAGATTTACATATTCGCCCGATGGAAGCGGTTAATCTGATTCGTGATTTGCTTGATGTCAAATCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAg                                                                                                                                                                                            >  1:183278/1‑249 (MQ=255)                          gCCGAGCACTGCCTCCGCCTTATTTGAATATCGCTTCAGCGGCAACGGCGAACTGGCGGGACACAATCTCGGCAATCTGATGCTAAAAGCATTGGAAGATTTACATATTCGCCCGATGGAAGCGGTTAATCTGATTCGTGATTTGCTTGATGTCAAATCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAg                                                                                                                                                                                            >  1:838601/1‑249 (MQ=255)                             gAGCACTGCCTCCGCCTTATTTGAATATCGCTTCAGCGGCAACGGCGAACTGGCGGGACACAATCTCGGCAATCTGATGCTAAAAGCATTGGAAGATTTACATATTCGCCCGATGGAAGCGGTTAATCTGATTCGTGATTTGCTTGATGTCAAATCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTg                                                                                                                                                                                                       >  1:694468/1‑235 (MQ=255)                              aGCACTGCCTCCGCCTTATTTGAATATCGCTTCAGCGGCAACGGCGAACTGGCGGGACACAATCTCGGCAATCTGATGCTAAAAGCATTGGAAGATTTACATATTCGCCCGATGGAAGCGGTTAATCTGATTCGTGATTTGCTTGATGTCAAATCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTaa                                                                                                                                                                                       >  1:529098/1‑250 (MQ=255)                               gCACTGCCTCCGCCTTATTTGAATATCGCTTCAGCGGCAACGGCGAACTGGCGGGACACAATCTCGGCAATCCGATGCTAAAAGCATTGGAAGATTTACATATTCGCCCGATGGAAGCGGTTAATCTGATTCGTGATTTGCTTGATGTCAAATCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGAc                                                                                                                                                                                              <  1:433402/242‑1 (MQ=255)                                cACTGCCTCCGCCTTATTTGAATATCGCTTCAGCGGCAACGGCGAACTGGCGGGACACAATCTCGGCAATCTGATGCTAAAAGCATTGGAAGATTTACATATTCGCCCGATGGAAGCGGTTAATCTGATTCGTGATTTGCTTGATGTCAAATCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAAc                                                                                                                                                                                      <  1:255643/249‑1 (MQ=255)                                    gCCTCCGCCTTATTTGAATATCGCTTCAGCGGCAACGGCGAACTGGCGGGACACAATCTCGGCAATCTGATGCTAAAAGCATTGGAAGATTTACATATTCGCCCGATGGAAGCGGTTAATCTGATTCGTGATTTGCTTGATGTCAAATCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGAGAGCGTGGACAGCTTACCCGaa                                                                                                                                                                                  >  1:421808/1‑249 (MQ=255)                                          gCCTTATTTGAATATCGCTTCAGCGGCAACGGCGAACTGGCGGGACACAATCTCGGCAATCTGATGCTAAAAGCATTGGAAGATTTACATATTCGCCCGATGGAAGCGGTTAATCTGATTCGTGATTTGCTTGATGTCAAATCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCg                                                                                                                                                                                                                     >  1:512357/1‑208 (MQ=255)                                              tATTTGAATATCGCTTCAGCGGCAACGGCGAACTGGCGGGACACAATCTCGGCAATCTGATGCTAAAAGCATTGGAAGATTTACATATTCGCCCGATGGAAGCGGTTAATCTGATTCGTGATTTGCTTGATGTCAAATCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCg                                                                                                                                                                                                                     >  1:848032/1‑204 (MQ=255)                                                           cTTCAGCGGCAACGGCGAACTGGCGGGACACAATCTCGGCAATCTGATGCTAAAAGCATTGGAAGATTTACATATTCGCCCGATGGAAGCGGTTAATCTGATTCGTGATTTGCTTGATGTCAAATCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAACCGAACTACCAAAATCCATTTTTCTTGtt                                                                                                                                                          >  1:288677/1‑250 (MQ=255)                                                           cTTCAGCGGCAACGGCGAACTGGCGGGACACAATCTCGGCAATCTGATGCTAAAAGCATTGGAAGATTTACATATTCGCCCGATGGAAGCGGTTAATCTGATTCGTGATTTGCTTGATGTCAAATCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAACCGAACTACCAAAATCCATTTTTCTTGtt                                                                                                                                                          >  1:856678/1‑250 (MQ=255)                                                                    cAACGGCGAACTGGCGGGACACAATCTCGGCAATCTGATGCTAAAAGCATTGGAAGATTTACATATTCGCCCGATGGAAGCGGTTAATCTGATTCGTGATTTGCTTGATGTCAAATCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAACCGAACTACCAAAATCCATTTTTCTTGTTCCCTATGTGc                                                                                                                                                <  1:61868/251‑1 (MQ=255)                                                                    cAACGGCGAACTGGCGGGACACAATCTCGGCAATCTGATGCTAAAAGCATTGGAAGATTTACATATTCGCCCGATGGAAGCGGTTAATCTGATTCGTGATTTGCTTGATGTCAAATCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAACCGAACTACCAAAATCCATTTTTCTTGTTCCCTATGTGc                                                                                                                                                <  1:646387/251‑1 (MQ=255)                                                                                ggCGGGACACAATCTCGGCAATCTGATGCTAAAAGCATTGGAAGATTTACATATTCGCCCGATGGAAGCGGTTAATCTGATTCGTGATTTGCTTGATGTCAAATCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAACCGAACTACCAAAATCCATTTTTCTTGTTCCCTATGTGCAAGCCACGCCgg                                                                                                                                    >  1:566211/1‑251 (MQ=255)                                                                                      acacAATCTCGGCAATCTGATGCTAAAAGCATTGGAAGATTTACATATTCGCCCGATGGAAGCGGTTAATCTGATTCGTGATTTGCTTGATGTCAAATCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAACCGAACTACCAAAATCCATTTTTCTTGTTCCCTATGTGCAAGCCACGCCGGAAGcc                                                                                                                               <  1:687230/250‑1 (MQ=255)                                                                                        acaATCTCGGCAATCTGATGCTAAAAGCATTGGAAGATTTACATATTCGCCCGATGGAAGCGGTTAATCTGATTCGTGATTTGCTTGATGTCAAATCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAACCGAACTACCAAAATCCATTTTTCTTGTTCCCTATGTGCAAGCCACGCCGGAAGCCAtt                                                                                                                            <  1:643848/251‑1 (MQ=255)                                                                                          aaTCTCGGCAATCTGATGCTAAAAGCATTGGAAGATTTACATATTCGCCCGATGGAAGCGGTTAATCTGATTCGTGATTTGCTTGATGTCAAATCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAg                                                                                                                                                                                                         <  1:115699/172‑1 (MQ=255)                                                                                          aaTCTCGGCAATCTGATGCTAAAAGCATTGGAAGATTTACATATTCGCCCGATGGAAGCGGTTAATCTGATTCGTGATTTGCTTGATGTCAAATCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCGCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAACCGAACTACCAAAATCCATTTTTCTTGTTCCCTATGTGCAAGCCACGCCGGAAGCCATTAg                                                                                                                          <  1:787250/251‑1 (MQ=255)                                                                                           aTCTCGGCAATCTGATGCTAAAAGCATTGGAAGATTTACATATTCGCCCGATGGAAGCGGTTAATCTGATTCGTGATTTGCTTGATGTCAAATCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAAc                                                                                                                                                                                      <  1:633828/190‑1 (MQ=255)                                                                                            tctcGGCAATCTGATGCTAAAAGCATTGGAAGATTTACATATTCGCCCGATGGAAGCGGTTAATCTGATTCGTGATTTGCTTGATGTCAAATCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAACCGAACTACCAAAATCCATTTTTCTTGTTCCCTATGTGCAAGCCACGCCGGAAGCCATTAgcg                                                                                                                        <  1:893863/251‑1 (MQ=255)                                                                                                        gATGCTAAAAGCATTGGAAGATTTACATATTCGCCCGATGGAAGCGGTTAATCTGATTCGTGATTTGCTTGATGTCAAATCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAACCGAACTACCAAAATCCATTTTTCTTGTTCCCTATGTGCAAGcc                                                                                                                                           <  1:337762/220‑1 (MQ=255)                                                                                                           gCTAAAAGCATTGGAAGATTTACATATTCGCCCGATGGAAGCGGTTAATCTGATTCGTGATTTGCTTGATGTCAAATCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAACCGAACTACCAAAATCCATTTTTCTTGTTCCCTATGTGCAAGCCACGCCGGAAGCCATTAgcg                                                                                                                        <  1:671163/236‑1 (MQ=255)                                                                                                                aaGCATTGGAAGATTTACATATTCGCCCGATGGAAGCGGTTAATCTGATTCGTGATTTGCTTGATGTCAAATCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAACCGAACTACCAAAATCCATTTTTCTTGTTCCCTATGTGCAAGCCACGCCGGAAGCCATTAgcg                                                                                                                        >  1:351802/1‑231 (MQ=255)                                                                                                                    aTTGGAAGATTTACATATTCGCCCGATGGAAGCGGTTAATCTGATTCGTGATTTGCTTGATGTCAAATCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAACCGAACTACCAAAATCCATTTTTCTTGTTCCCTATGTGCAAGCCACGCCGGAAGCCATTAGCGCATTAAAAGAAGCTGAAGTg                                                                                                    <  1:463617/247‑1 (MQ=255)                                                                                                                         aaGATTTACATATTCGCCCGATGGAAGCGGTTAATCTGATTCGTGATTTGCTTGATGTCAAATCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAACCGAACTACCAAAATCCATTTTTCTTGTTCCCTATGTGCAAGCCACGCCGGAAGCCATTAGCGCATTAAAAGAAGCTGAAGTGATTTTAtt                                                                                            <  1:613006/250‑1 (MQ=255)                                                                                                                         aaGATTTACATATTCGCCCGATGGAAGCGGTTAATCTGATTCGTGATTTGCTTGATGTCAAATCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAACCGAACTACCAAAATCCATTTTTCTTGTTCCCTATGTGCAAGCCACGCCGGAAGCCATTAGCGCATTAAAAGAAGCTGAAGTGATTTTAtt                                                                                            <  1:589021/250‑1 (MQ=255)                                                                                                                           gATTTACATATTCGCCCGATGGAAGCGGTTAATCTGATTCGTGATTTGCTTGATGTCAAATCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAACCGAACTACCAAAATCCATTTTTCTTGTTCCCTATGTGCAAGCCACGCCGGAAGCCATTAGCGCATTAAAAGAAGCTGAAGTGATTTTATTCgg                                                                                         >  1:474482/1‑251 (MQ=255)                                                                                                                                  atatTCGCCCGATGGAAGCGGTTAATCTGATTCGTGATTTGCTTGATGTCAAATCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAACCGAACTACCAAAATCCATTTTTCTTGTTCCCTATGTGCAAGCCACGCCGGAAGCCATTAGCGCATTAAAAGAAGCTGAAGTGATTTTATTCGGTCCCGGc                                                                                  <  1:900692/251‑1 (MQ=255)                                                                                                                                   tatTCGCCCGATGGAAGTGGTTAATCTGATTCGTGATTTGCTTGATGTCAAATCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAACCGAACTACCAAAATCCATTTTTCTTGTTCCCTAtgtg                                                                                                                                                 <  1:451205/187‑1 (MQ=255)                                                                                                                                                                          gCTTGATGTCAAATCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAACCGAACTACCAAAATCCATTTTTCTTGTTCCCTATGTGCAAGCCACGCCGGAAGCCATTAGCGCATTAAAAGAAGCTGAAGTGATTTTATTCGGTCCCGGCAGTTTTTTAACCAGCATTCTAccgccg                                                       <  1:73299/238‑1 (MQ=255)                                                                                                                                                                               aTGTCAAATCCTTCATCATCCCAATGTCGGAGCGGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCGGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAACCGAACTACAAAAATCCATTTTTCTTGTTCCCTATGTGCAAGCCACGCCGGAAGCCATTAGCGCATTAAAAGAAGCTGAAGTGATTTTATTCGGTCCCGGCAGTTTTTTAACCAGCATTCTACCGCCGATTCTCTTGTCTGAAGt                                      <  1:47959/250‑1 (MQ=255)                                                                                                                                                                                tGTCAAATCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAACCGAACTACCAAAATCCATTTTTCTTGTTCCCTATGTGc                                                                                                                                                >  1:253403/1‑143 (MQ=255)                                                                                                                                                                                tGTCAAATCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAACCGAACTACCAAAATCCATTTTTCTTGTTCCCTATGTGCAAGCCACGCCGGAAGCCATTAGCGCATTAAAAGAAGCTGAAGTGATTTTATTCGGTCCCGGCAGTTTTTTAACCAGCATTCTACCGCCGATTCTCTTGTCTGAAGTCa                                    >  1:561510/1‑251 (MQ=255)                                                                                                                                                                                 gTCAAATCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAAc                                                                                                                                                                                      >  1:262453/1‑104 (MQ=255)                                                                                                                                                                                 gTCAAATCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAACCGAACTACCAAAATCCATTTTTCTTGTTCCCTATGTGCAAGCCACGCCGGAAGCCATTAGCGCATTAAAAGAAGCTGAAGTGATTTTATTCGGTCCCGGCAGTTTTTTAACCAGCATTCTACCGCCGATTCTCTTGTCTGAAGTCAt                                   >  1:116415/1‑251 (MQ=255)                                                                                                                                                                                      aTCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAACCGAACTACCAAAATCCATTTTTCTTGTTCCCTATGTGCAAGCCACGCCGGAAGCCATTAGCGCATTAAAAGAAGCTGAAGTGATTTTATTCGGTCCCGGCAGTTTTTTAACCAGCATTCTACCGCCGATTCTCTTg                                              <  1:1132/235‑1 (MQ=255)                                                                                                                                                                                       tCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAACCGAACTACCAAAATCCATTTTTCTTGTTCCCTATGTGCAAGCCACGCCGGAAGCCATTAGCGCATTAAAAGAAGCTGAAGTGATTTTATTCGGTCCCGGCAGTTTTTTAACCAGCATTCTACCGCCGATTCTCTTGTCTGAAGTCATTGaaca                             >  1:531771/1‑251 (MQ=255)                                                                                                                                                                                       tCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAACCGAACTACCAAAATCCATTTTTCTTGTTCCCTATGTGCAAGCCACGCCGGAAGCCATTAGCGCATTAAAAGAAGCTGAAGTGATTTTATTCGGTCCCGGCAGTTTTTTAACCAGCATTCTACCGCCGATTCTCTTGTCTGAAGTCATTGaaca                             >  1:254502/1‑251 (MQ=255)                                                                                                                                                                                            catcatCCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAACCGAACTACCAAAATCCATTTTTCTTGTTCCCTATGTGCAAGCCACGCCGGAAGCCATTAGCGCATTAAAAGAAGCTGAAGTGATTTTATTCGGTCCCGGCAGTTTTTTAACCAGCATTCTACCGCCGATtctc                                                 >  1:509068/1‑226 (MQ=255)                                                                                                                                                                                              tcatcCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAACCGAACTACCAAAATCCATTTTTCTTGTTCCCTATGTGCAAGCCACGCCGGAAGCCATTAGCGCATTAAAAGAAGCTGAAGTGATTTTATTCGGTCCCGGCAGTTTTTTAACCAGCATTCTACCGCCGATTCTCTTGTCTGAAg                                       >  1:790430/1‑234 (MQ=255)                                                                                                                                                                                               catcCCAAGGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAACCGAACTACCAAAATCCATTTTTCTTGTTCCCTATGTGCAAGCCACGCCGGAAGCCATTAGCGCATTAAAAGAAGCTGAAGTGATTTTATTCGGTCCCGGCAGTTTTTTAACCAGCATTCTACCGCCGATTCTCTTGTCTGAAGTCATTGAACAACTGcaac                     <  1:214605/251‑1 (MQ=255)                                                                                                                                                                                                atcCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAACCGAACTACCAAAATCCATTTTTCTTGTTCCCTATGTGCAAGCCACGCCGGAAGCCATTAGCGCATTAAAAGAAGCTGAAGTGATTTTATTCGGTCCCGGCAGTTTTTTAACCAGCATTCTACCGCCGATTCTCTTGTCTGAAGTCATTGAACAACTGcaaca                    >  1:378260/1‑251 (MQ=255)                                                                                                                                                                                                atcCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAACCGAACTACCAAAATCCATTTTTCTTGTTCCCTATGTGCAAGCCACGCCGGAAGCCATTAGCGCATTAAAAGAAGCTGAAGTGATTTTATTCGGTCCCGGCAGTTTTTTAACCAGCATTCTACCGCCGATTCTCTTGTCTGAAGTCATTGAACAACTGcaaca                    <  1:245270/251‑1 (MQ=255)                                                                                                                                                                                                 tcCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAACCGAACTACCAAAATCCATTTTTCTTGTTCCCTATGTGCAAGCCACGCCGGAAGCCATTAGCGCATTAAAAGAAGCTGAAGTGATTTTATTCGGTCCCGGCAGTTTTTTAACCAGCATTCTAc                                                            >  1:473768/1‑210 (MQ=255)                                                                                                                                                                                                 tcCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAACCGAACTACCAAAATCCATTTTTCTTGTTCCCTATGTGCAAGCCACGCCGGAAGCCATTAGCGCATTAAAAGAAGCTGAAGTGATTTTATTCGGTCCCGGCAGTTTTTTAACCAGCATTCTACCGCCGATTCTCTTGTCTGAAGTCATTGaac                              <  1:510872/240‑1 (MQ=255)                                                                                                                                                                                                  cccAATGTCGGAGCAGCCCGCGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAACCGAACTACCAAAATCCATTTTTCTTGTTCCCTATGTGCAAGCCACGCCGGAAGCCATTAGCGCATTAAAAGAAGCTGAAGTGATTTTATTCGGTCTCGGCAGTTTTTTAACCAGCATTCTACCGCCGATTCTCTTGTCTGAAGTCATTGAACAACTGCAACaaa                  >  1:463869/1‑251 (MQ=255)                                                                                                                                                                                                          cGGAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAACCGAACTACCAAAATCCATTTTTCTTGTTCCCTATGTGCAAGCCACGCCGGAAGCCATTAGCGCATTAAAAGAAGCTGAAGTGATTTTATTCGGTCCCGGCAGTTTTTTAACCAGCATTCTACCGCCGATTCTCTTGTCTGAAGTCATTGAACAACTGCAACAaagccaagcc          <  1:353660/251‑1 (MQ=255)                                                                                                                                                                                                            gAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAACCGAACTACCAAAATCCATTTTTCTTGTTCCCTATGTGCAAGCCACGCCGGAAGCCATTAGCGCATTAAAAGAAGCTGAAGTGATTTTATTCGGTCCCGGCAGTTTTTTAACCAGCATTCTACCGCCGATTCTCTTGTCTGAAGTCATTGAACAACTGCAACAAAgccaagccaa        >  1:332154/1‑251 (MQ=255)                                                                                                                                                                                                            gAGCAGCCCGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAACCGAACTACCAAAATCCATTTTTCTTGTTCCCTATGTGCAAGCCACGCCGGAAGCCATTAGCGCATTAAAAGAAGCTGAAGTGATTTTATTCGGTCCCGGCAGTTTTTTAACCAGCATTCTACCGCCGATTCTCTTGTCTGAAGTCATTGAACAACTGCAACAAAgccaagccaa        >  1:34309/1‑251 (MQ=255)                                                                                                                                                                                                                  cccGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAACCGAACTACCAAAATCCATTTTTCTTGTTCCCTATGTGCAAGCCACGCCGGAAGCCATTAGCGCATTAAAAGAAGCTGAAGTGATTTTATTCGGTCCCGGCAGTTTTTTAACCAGCATTCTACCGCCGATTCTCTTGTCTGAAGTCATTGaacaac                           >  1:128844/1‑226 (MQ=255)                                                                                                                                                                                                                  cccGTGCATCTCGCCGCCATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAACCGAACTACCAAAATCCATTTTTCTTGTTCCCTATGTGCAAGCCACGCCGGAAGCCATTAGCGCATTAAAAGAAGCTGAAGTGATTTTATTCGGTCCCGGCAGTTTTTTAACCAGCATTCTACCGCCGATTCTCTTGTCTGAAGTCATTGAACAACTGCAACAAAGCCAAGCCAAGAAAAt  >  1:519608/1‑251 (MQ=255)                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                          ATTGTTTAAATCAAATCATTACGCAGCCGAGCACTGCCTCCGCCTTATTTGAATATCGCTTCAGCGGCAACGGCGAACTGGCGGGACACAATCTCGGCAATCTGATGCTAAAAGCATTGGAAGATTTACATATTCGCCCGATGGAAGCGGTTAATCTGATTCGTGATTTGCTTGATGTCAAATCCTTCATCATCCCAATGTCGGAGCAGCCCGTGCATCTCGCCGCTATTTCAGGCAGCGGCGCCAGCGTGGTAGGCGAAGTGAGCGTGGACAGCTTAACCGAACTACCAAAATCCATTTTTCTTGTTCCCTATGTGCAAGCCACGCCGGAAGCCATTAGCGCATTAAAAGAAGCTGAAGTGATTTTATTCGGTCCCGGCAGTTTTTTAACCAGCATTCTACCGCCGATTCTCTTGTCTGAAGTCATTGAACAACTGCAACAAAGCCAAGCCAAGAAAAT  >  1/1159720‑1160179

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑