Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,158,079	G→A	A15V (GCC→GTC)	unknown ←	Cyclic pyranopterin monophosphate synthase (EC 4.6.1.17)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,158,079	0	G	A	89.1%	144.2 / ‑2.9	65	A15V (GCC→GTC)	unknown	Cyclic pyranopterin monophosphate synthase (EC 4.6.1.17)
Reads supporting (aligned to +/- strand):  ref base G (0/1);  major base A (35/22);  minor base C (0/7);  total (35/30)
Fisher's exact test for biased strand distribution p-value = 2.51e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

AAGCAATAACGGATGGCACAAGGGAATCAATTCCCACGTGCGTTTTGCCGCCTGAATGCCGGCAATGCGGGCGGTGGCGAACACATCGCCTTTGTGATGCTGACCGGACAGGATCATTTGTAAGGTTTCGGGCGCCATACAAACATAAGCCTCAGCGCGGGCTTCACGGACGGTTTCGGCTTT‑GGCGGAAACGTCCACCATATTGGCTTCCCC‑ATGGG‑AA‑TTAAT‑GTGAGTAAATGTGG‑CCATGATAATTAAAGTGCGGTTGA‑TTTTCTTGCTGTTTTTC‑AAAAT‑CGGCGTGCATTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGTTCACGCTTACCTTGTAACGCAGAAAAAATTCTTGATTGCAAAATGAGTTGCTGCTTATCGTATTGTAACAAATCGCGTAATTCAATGCCTTCCTCGCCGAATAAACAAAGGTGCAATTTC  >  1/1157841‑1158318                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    aaGCAATAACGGATGGCACAAGGGAATCAATTCCCACGTGCGTTTTGCCGCCTGAATGCCGGCAATGCGGGCGGTGGCGAACACATCGCCTTTGTGATGCTGACCGGACAGGATCATTTGTAAGGTTTCGGGCGCCATACAAACATAAGCCTCAGCGCGGGCTTCACGGACGGTTTCGGCTTT‑GGCGGAAACGTCCACCATATTGGCTTCCCC‑ATGGG‑AA‑TTAAT‑GTGAGTAAATGTGA‑CCATGATAATTa                                                                                                                                                                                                                                        <  1:900931/251‑1 (MQ=255)    cAATAACGGATGGCACAAGGGAATCAATTCCCACGTGCGTTTTGCCGCCTGAATGCCGGCAATGCGGGCGGTGGCGAACACATCGCCTTTGTGATGCTGACCGGACAGGATCATTTGTAAGGTTTCGGGCGCCATACAAACATAAGCCTCAGCGCGGGCTTCACGGACGGTTTCGGCTTT‑GGCGGAAACGTCCACCATATTGGCTTCCCC‑ATGGG‑AA‑TTAAT‑GTGAGTAAATGTGA‑CCATGATAATTAAAg                                                                                                                                                                                                                                     <  1:202925/251‑1 (MQ=255)              tGGCACAAGGGAATCAATTCCCACGTGCGTTTTGCCGCCTGAATGCCGGCAATGCGGGCGGTGGCGAACACATCGCCTTTGTGATGCTGACCGGACAGGATCATTTGTAAGGTTTCGGGCGCCATACAAACATAAGCCTCAGCGCGGGCTTCACGGACGGTTTCGGCTTT‑GGCGGAAACGTCCACCATATTGGCTTCCCC‑ATGGG‑AA‑TTAAT‑GTGAGTAAATGTGA‑CCATGATAATTAAAGTGCGGTTGA‑t                                                                                                                                                                                                                          >  1:105522/1‑251 (MQ=255)                gCACAAGGGAATCAATTCCCACGTGCGTTTTGCCGCCTGAATGCCGGCAATGCGGGCGGTGGCGAACACATCGCCTTTGTGATGCTGACCGGACAGGATCATTTGTAAGGTTTCGGGCGCCATACAAACATAAGCCTCAGCGCGGGCTTCACGGACGGTTTCGGCTTT‑GGCGGAAACGTCCACCATATTGGCTTCCCC‑ATGGG‑AA‑TTAAT‑GTGAGTAAATGTGA‑CCATGATAATTAAAGTGCGGTTGA‑ttt                                                                                                                                                                                                                        >  1:24972/1‑251 (MQ=255)                            cAATTCCCACGTGCGTTTTGCCGCCTGAATGCCGGCAATGCGGGCGGTGGCGAACACATCGCCTTTGTGATGCTGACCGGACAGGATCATTTGTAAGGTTTCGGGCGCCATACAAACATAAGCCTCAGCGCGGGCTTCACGGACGGTTTCGGCTTT‑GGCGGAAACGTCCACCATATTGGCTTCCCC‑ATGGG‑AA‑TTAAT‑GTGAGTAAATGTGA‑CCATGATAATTAAAGTGCGGTTGA‑TTTTCTTGCTGtttt                                                                                                                                                                                                            >  1:750914/1‑251 (MQ=255)                              aTTCCCACGTGCGTTTTGCCGCCTGAATGCCGGCAATGCGGGCGGTGGCGAACACATCGCCTTTGTGATGCTGACCGGACAGGATCATTTGTAAGGTTTCGGGCGCCATACAAACATAAGCCTCAGCGCGGGCTTCACGGACGGTTTCGGCTTT‑GGCGGAAACGTCCACCATATTGGCTTCCCC‑ATGGG‑AA‑TTAAT‑GTGAGTAAATGTGA‑CCATGATAATTAAAGTGCGGTTGA‑TTTTCTTGCTGttttt                                                                                                                                                                                                           >  1:62263/1‑250 (MQ=255)                                             ttGCCGCCTGAATGCCGGCAATGCGGGCGGTGGCGAACACATCGCCTTTGTGATGCTGACCGGACAGGATCATTTGTAAGGTTTCGGGCGCCATACAAACATAAGCCTCAGCGCGGGCTTCACGGACGGTTTCGGCTTT‑GGCGGAAACGTCCACCATATTGGCTTCCCC‑ATGGG‑AA‑TTAAT‑GTGAGTAAATGTGA‑CCATGATAATTAAAGTGCGGTTGA‑TTTTCTTGCTGTTTTTC‑AAAAT‑CGGCGTGCAt                                                                                                                                                                                         <  1:557684/251‑1 (MQ=255)                                                 cgccTGAATGCCGGCAATGCGGGCGGTGGCGAACACATCGCCTTTGTGATGCTGACCGGACAGGATCATTTGTAAGGTTTCGGGCGCCATACAAACATAAGCCTCAGCGCGGGCTTCACGGACGGTTTCGGCTTT‑GGCGGAAACGTCCACCATATTGGCTTCCCC‑ATGGG‑AA‑TTAAT‑GTGAGTAAATGTGA‑CCATGATAATTAAAGTGCGGTTGA‑TTTTCTTGCTGTTTTTC‑AAAAT‑CGGCGTGCATTAAc                                                                                                                                                                                     <  1:69813/251‑1 (MQ=255)                                                   ccTGAATGCCGGCAATGCGGGCGGTGGCGAACACATCGCCTTTGTGATGCTGACCGGACAGGATCATTTGTAAGGTTTCGGGCGCCATACAAACATAAGCCTCAGCGCGGGCTTCACGGACGGTTTCGGCTTT‑GGCGGAAACGTCCACCATATTGGCTTCCCC‑ATGGG‑AA‑TTAAT‑GTGAGTAAATGTGA‑CCATGATAATTAAAGTGCGGTTGA‑TTTTCTTGCTGTTTTTC‑AAAAT‑CGGCGTGCATTAAcc                                                                                                                                                                                    <  1:376771/250‑1 (MQ=255)                                                                   gcgggcggTGGCGAACACATCGCCTTTGTGATGCTGACCGGACAGGATCATTTGTAAGGTTTCGGGCGCCATACAAACATAAGCCTCAGCGCGGGCTTCACGGACGGTTTCGGCTTT‑GGCGGAAACGTCCACCATATTGGCTTCCCC‑ATGGG‑AA‑TTAAT‑GTGAGTAAATGTGA‑CCATGATAATTAAAGTGCGGTTGA‑TTTTCTTGCTGTTTTTC‑AAAAT‑CGGCGTGCATTAAc                                                                                                                                                                                     <  1:372039/233‑1 (MQ=255)                                                                         ggTGGCAACCACTTCGCTTTTGTGATGCTGCCCGGACAGGATCTTTTGTAAGGTTTCGGGCGCCAAACAAACAAAAGCCTCGGCGCGGGCTTCACGGACGGTTTCGGTTTT‑GGCGGAAACGCCCACCATATTGGCTCCCCC‑ATGGA‑AA‑TTAAT‑GTGAGAAAATGTGA‑CCATGATAATAAAAGTGGGGTTGT‑TTTTCTTGTTTTTTTTA‑AAAAT‑CGGGGTGCATTAACCGCCGATACTTGCCAAATGACta                                                                                                                                                              <  1:281306/250‑1 (MQ=255)                                                                             gCGAACACTCCGCTTTTGTGATGCTGACCGGACAGGATCTTTTGTAAGTTTTGGGGCGCCATAAAAACATAACCCTCAGCGCGGGCTTCACGGAGGTTTTCGGTTTG‑GGCGGAAACGTCCACCATTTTGGCTTCCCA‑ATGGG‑AA‑TTATT‑GTGATTAAATGTGA‑CCATAATAATAAAGGTGCGTTTTT‑TTTTTTTGTTTTTTTTA‑AAAAT‑CGGCGTGCATTAACCGCCGATACTTGCCAAATGACtacgta                                                                                                                                                          <  1:487616/250‑1 (MQ=255)                                                                                        gCCTTTGTGATGCTGACCGGACAGGATCATTTGTAAGGTTTCGGGCGCCATACAAACATAAGCCTCAGCGCGGGCTTCACGGACGGTTTCGGCTTT‑GGCGGAAACGTCCACCATATTGGCTTCCCC‑ATGGG‑AA‑TTAAT‑GTGAGTAAATGTGA‑CCATGATAATTAAAGTGCGGTTGA‑TTTTCTTGCTGTTTTTC‑AAAAT‑CGGCGTGCATTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCg                                                                                                                                               >  1:763609/1‑250 (MQ=255)                                                                                         cagttGTGATGCTGACCGGACAGGATCATTTGTAAGGTTTCGGGCGCCATACAAACATAAGCCTCAGCGCGGGCTTCACGGACGGTTTCGGCTTT‑GGCGGAAACGTCCACCATATTGGCTTCCCC‑ATGGG‑AA‑TTAAT‑GTGAGTAAATGTGA‑CCATGATAATTAAAg                                                                                                                                                                                                                                     >  1:257776/4‑166 (MQ=255)                                                                                                     tGACCGGACAGGATCATTTGTAAGGTTTCGGGCGCCATACAAACATAAGCCTCAGCGCGGGCTTCACGGACGGTTTCGGCTTT‑GGCGGAAACGTCCACCATATTGGCTTCCCC‑ATGGG‑AA‑TTAAT‑GTGAGTAAATGTGA‑CCATGATAATTAAAGTGCGGTTGA‑tt                                                                                                                                                                                                                         <  1:639285/165‑1 (MQ=255)                                                                                                           gACAGGATTTTTTGTAAGGTTGCGGGCGCAATAAAAAAATAAGCCTCGGCGGGGGCTTCAGGGAGGGTTTCGGTTTT‑GGCGAAAACGCCCACCATATGGGCTCCCCC‑AGGGG‑AA‑TTAAT‑GTGAGTAAATGTGC‑CCATGATAATAAAAGTGCGGTTGA‑TTTTTTTGCTGTTTTAA‑AAAAT‑CGGCGTGCATAAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAatgat                                                                                                                            <  1:592206/250‑1 (MQ=255)                                                                                                               ggATCTTTTGAAAGGTTGCGGGCGCAATAAAAACAAAAGCCTCGGCGGGGGCTTCACGGACGTTTTCGGTTTT‑GGCGGAAACGTCCACCATTTTGGCTCCCCC‑ATGGA‑AA‑TTAAT‑GTGAGTAAATGTGC‑CCATAATAATTAAAGTGCGTTTGA‑TTTTCTTGCTTTTTTTA‑AAAAC‑CGGCGTGCATTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGTTc                                                                                                                        <  1:68246/250‑1 (MQ=255)                                                                                                               ggATCATTTGTAAGGTTTCGGGCGCCATACAAACATAAGCCTCAGCGCGGGCTTCACGGACGGTTTCGGCTTT‑GGCGGAAACGTCCACCATATTGGCTTCCCC‑ATGGG‑AA‑TTAAT‑GTGAGTAAATGTGA‑CCATGATAATTAAAGTGCGGTTGA‑TTTTCTTGCTGTTTTTC‑AAAAT‑CGGCGTGCATTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGTTCa                                                                                                                       >  1:491158/1‑251 (MQ=255)                                                                                                                gATCATTTGTAAGGTTTCGGGCGCCATACAAACATAAGCCTCAGCGCGGGCTTCACGGACGGTTTCGGCTTT‑GGCGGAAACGTCCACCATATTGGCTTCCCC‑ATGGG‑AA‑TTAAT‑GTGAGTAAATGTGA‑CCATGATAATTAAAGTGCGGTTGA‑TTTTCTTGCTGTTTTTC‑AAAAT‑CGGCGTGCATTAACCGCCGATACTTGCCAAATg                                                                                                                                                                  >  1:887906/1‑207 (MQ=255)                                                                                                                   cATTTGTAGGGTTTGGGGCCCCATAAAAACATAAGCCTCGGCGGGGGCTTCACGGAGGGTTGCGGTTTT‑GGGGGAACCGTCTACCATTTTGGCTCCCCC‑ATGGA‑AA‑TTAAT‑GTGAGAAAATGTAA‑CCATGAAAATAAAAGTGGGTTTGT‑TTTTTTTGTTTTTTTAA‑AAAAT‑CGGGGTGAATTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGTTCACGCt                                                                                                                   <  1:438616/251‑1 (MQ=255)                                                                                                                     tttGTAAGGTTTCGGGCGCCATACAAACATAAGCCTCAGCGCGGGCTTCACGGACGGTTTCGGCTTT‑GGCGGAAACGTCCACCATATTGGCTTCCCC‑ATGGG‑AA‑TTAAT‑GTGAGTAAATGTGA‑CCATGATAATTAAAGTGCGGTTGA‑TTTTCTTGCTGTTTTTC‑AAAAT‑CGGCGTGCATTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGTTCACGCTTa                                                                                                                 >  1:194584/1‑251 (MQ=255)                                                                                                                         tagggTTTCGGGCGCCATACAAACATAAGCCTCAGCGCGGGCTTCACGGACGGTTTCGGTTTT‑GGCGGAAACCTCCACAATATTGGCTCCCCC‑AGGGA‑AA‑TTAAT‑GTGAGAAAATGTGA‑CCATAATAATAAAAGTGCGGTTGT‑TTTTCTTGTTTTTTTTA‑AAAAT‑CGGCGTGCATTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGTTCACGCTTACCt                                                                                                              <  1:794017/247‑1 (MQ=255)                                                                                                                         aaaGGTTTGGGGCACTATAAAACCAAAAGCCTCAGCGCGGGCTACGCGAAGGGTTTGGGTTTG‑GGCGGAACCGTCCACGATTTTGG‑TTCCCCTAGGGA‑AA‑TTATG‑GTGATTAATTGTGA‑CCATGATAATAAAAGTG‑GGTTTATTTTTCTGGCTTTTTTTA‑AAAAT‑GGGGGTGTATAAGCCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGTTCACGCTTACCt                                                                                                              <  1:327155/249‑1 (MQ=255)                                                                                                                                         aTACAAACATAAGCCTCAGCGCGGGCTTCACGGACGGTTTCGGCTTT‑GGCGGAAACGTCCACCATATTGGCTTCCCC‑ATGGG‑AA‑TTAAT‑GTGAGTAAATGTGA‑CCATGAt                                                                                                                                                                                                                                             <  1:649995/110‑1 (MQ=255)                                                                                                                                         aTACAAACATAAGCCTCAGCGCGGGCTTCACGGACGGTTTCGGCTTT‑GGCGGAAACGTCCACCATATTGGCTTCCCC‑ATGGG‑AA‑TTAAT‑GTGAGTAAATGTGA‑CCATGATAATTAAAGTGCGGTTGA‑TTTTCTTGCTGTTTTTC‑AAAAT‑CGGCGTGCATTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGTTCACGCTTACCTTGTAACGcctgtttctt                                                                                             >  1:772694/1‑242 (MQ=255)                                                                                                                                         aTACAAACATAAGCCTCAGCGCGGGCTTCACGGACGGTTTCGGCTTT‑GGCGGAAACGTCCACCATATTGGCTTCCCC‑ATGGG‑AA‑TTAAT‑GTGAGTAAATGTGA‑CCATGATAATTAAAGTGCGGTTGA‑TTTTCTTGCTGTTTTTC‑AAAAT‑CGGCGTGCATTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGTTCACGCTTACCTTGTAACGCAGAAAAAAt                                                                                             >  1:430650/1‑251 (MQ=255)                                                                                                                                              aaCATAAGCCTCAGCGCGGGCTTCACGGACGGTTTCGGCTTT‑GGCGGAAACGTCCACCATATTGGCTTCCCC‑ATGGG‑AA‑TTAAT‑GTGAGTAAATGTGA‑CCATGATAATTAAAGTGCGGTTGA‑TTTTCTTGCTGTTTTTC‑AAAAT‑CGGCGTGCATTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGTTCACGCTTACCTTGTAACGCAGAAAAAATTCTTg                                                                                        >  1:312210/1‑251 (MQ=255)                                                                                                                                               aCATAAGCCTCAGCGCGGGCTTCACGGACGGTTTCGGCTTT‑GGCGGAAACGTCCACCATATTGGCTTCCCC‑ATGGG‑AA‑TTAAT‑GTGAGTAAATGTGA‑CCATGATAATTACAGTGCGGTTGA‑TTTTCTTGCTGTTTTTC‑AAAAT‑CGGCGTGCATTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGTTCACGCTTACCt                                                                                                              >  1:58484/1‑228 (MQ=255)                                                                                                                                               aCATAAGCCTCAGCGCGGGCTTCACGGACGGTTTCGGCTTT‑GGCGGAAACGTCCACCATATTGGCTTCCCC‑ATGGG‑AA‑TTAAT‑GTGAGTAAATGTGA‑CCATGATAATTAAAGTGCGGTTGA‑TTTTCTTGCTGTTTTTC‑AAAAT‑CGGCGTGCATTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGTTCACGCTTACCTTGTAACGCAGAAAAAATTCTTGa                                                                                       >  1:375268/1‑251 (MQ=255)                                                                                                                                                cATAAGCCTCAGCGCGGGCTTCACGGACGGTTTCGGCTTT‑GGCGGAAACGTCCACCATATTGGCTTCCCC‑ATGGG‑AA‑TTAAT‑GTGAGTAAATGTGA‑CCATGATAATTAAAGTGCGGTTGA‑TTTTCTTGCTGTTTTTC‑AAAAT‑CGGCGTGCATTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGc                                                                                                                                      >  1:94239/1‑203 (MQ=255)                                                                                                                                                cATAAGCCTCAGCGCGGGCTTCACGGACGGTTTCGGCTTT‑GGCGGAAACGTCCACCATATTGGCTTCCCC‑ATGGG‑AA‑TTAAT‑GTGAGTAAATGTGA‑CCATGATAATTAAAGTGCGGTTGA‑TTTTCTTGCTGTTTTTC‑AAAAT‑CGGCGTGCATTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAat                                                                                                                               >  1:97899/1‑210 (MQ=255)                                                                                                                                                     gCCTCAGCGCGGGCTTCACGGACGGTTTCGGCTTT‑GGCGGAAACGTCCACCATATTGGCTTCCCC‑ATGGG‑AA‑TTAAT‑GTGAGTAAATGTGA‑CCATGATAATTAAAGTGCGGTTGA‑TTTTCTTGCTGTTTTTC‑AAAAT‑CGGCGTGCATTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGTTCACGCTTACCt                                                                                                              >  1:718695/1‑222 (MQ=255)                                                                                                                                                         cAGCGCGGGCTTCACGGACGGTTTCGGCTTT‑GGCGGAAACGTCCACCATATTGGCTTCCCC‑ATGGG‑AA‑TTAAT‑GTGAGTAAATGTGA‑CCATGATAATTAAAGTGCGGTTGA‑TTTTCTTGCTGTTTTTC‑AAAAT‑CGGCGTGCATTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGTTCACGCTTACCTTGTAACGCAGAAAAAATTCTTGATTGCAAAATg                                                                             >  1:400091/1‑251 (MQ=255)                                                                                                                                                           gcgggggCTTCAGGGACGGTTTCGGTTTG‑GGCGAAAACGCCCACCATATGGGCTCCCCC‑ATGGG‑AA‑TAAAT‑GTGAGTAATTGTGA‑CAATGATAATAAAGGTGCGGTTGT‑TTTTCTTGCTTTTTTTA‑AAAAT‑CGGCGGGCTTTAACCGCCGATACTTGCCAAATGACTACGTACGCCAc                                                                                                                                                    <  1:873162/174‑1 (MQ=255)                                                                                                                                                                    tCACGGACGGTTTCGGCTTT‑GGCGGAAACGTCCACCATATTGGCTTCCCC‑ATGGG‑AA‑TTAAT‑GTGAGTAAATGTGA‑CCATGATAATTAAAGTGCGGTTGA‑TTTTCTTGCTGTTTTTC‑AAAAT‑CGGCGTGCATTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGt                                                                                                                          >  1:806019/1‑195 (MQ=255)                                                                                                                                                                          aggttttCGGTTTT‑GGCGGAAACGTCCACAATATTGGCTCCCCC‑AGGGA‑AA‑TTAAT‑GTGAGTAAATGTGC‑CAATGATAATAAAAGTGCGGTTGT‑TTTTTTTGCTTTTTTTA‑AAAAT‑CGGCGTGAATTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGTTCACGCTTACCTTGTAACGCAGAAAAAATTCTTGATTGCAAAATGAGTTGCTGCTTATCGt                                                             <  1:114468/246‑1 (MQ=255)                                                                                                                                                                          acgttttCGGTTTT‑GGCGAAAACGCCCACCATATTGGCTTCCCC‑ATGGA‑AA‑TTAAT‑GTGAGTAAATGTGA‑CCATGATAATTAAAGTGCGGTTGA‑TTTTCTTGTTGTTTTTC‑AAAAT‑CGGCGTGCATAAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGTTCACGCTTACCTTGTAACGCAGAAAAAATTCTTGATTGCAAAATGAGTTGCTGCTTATCGt                                                             <  1:588873/246‑1 (MQ=255)                                                                                                                                                                          acggTTTCGGTTTT‑GGCGGAACCGCCCACCATATTGGCTCCCCC‑ATGGA‑AA‑TTAAT‑GTGAGAAAATGTGA‑CAATGATAATAAAAGTGCGTTTGT‑TTTTCTTGTTTTTTTTA‑AAAAT‑CGGCGTGCATTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGAAGTGCAGATAATGATGTTCACGCTTACCTTGTAACGCAGAAAAAATTCTTGATTGCAAAATGAGTTGCTGCTTATCGt                                                             <  1:15591/250‑1 (MQ=255)                                                                                                                                                                           cggTTTCGGGTTTGGGAGGAACCTTCCACCATATGGGC‑TCCCC‑AAGGG‑GA‑TTTATGGTGAGTAAATGTGC‑CAAGGATAATTAAAG‑GGGGTTTT‑TTTTTTTGGTTTTTTTA‑AAAATGCGGGTTGAATAAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGTTCACGCTTACCTTGTAACGCAGAAAAAATTCTTGATTGCAAAATGAGTTGCTGCTTATCGt                                                             <  1:363804/250‑1 (MQ=255)                                                                                                                                                                                tCGGCTTG‑GGGGGAACCGTCCACCATATTGGTTCCCCC‑AGGGG‑AT‑TTAAT‑GTGA‑TTAATGGGGACCATGATAATGAAAGTGGGGTTGA‑TTTTTTTCTTGTTTTAA‑AAAAC‑GGGGGTGCATAAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGTTCACGCTTACCTTGTAACGCAGAAAAAATTCTTGATTGCAAAATGAGTTGCTGCTTATCGTATTGTaa                                                      <  1:261139/251‑1 (MQ=255)                                                                                                                                                                                 cgttttt‑GGCGGAACCTTCCACCTTTTTGCCTTCCCC‑A‑GGG‑AATTTAAT‑GTGATTAATTTTGA‑CCATTATATTTAAGGTGGGTTTTT‑TTTTTTTGCT‑TTTTTCAAAAAT‑CGGCGTGAATTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGTTCACGCTTACCTTGTAACGCAGAAAAAATTCTTGATTGCAAAATGAGTTGCTGCTTATCGTATTGTAAc                                                     <  1:466331/247‑1 (MQ=255)                                                                                                                                                                                    gtttgggcggaacctcccaccaTTTTGCC‑TCCCC‑AAGGGAAA‑TAAAT‑GTAAGTAAATGTGC‑CCATGATAATTAAAGTGCGGTTGT‑TTTTCTTGTTTTTTTTA‑AAAGT‑GGGCGTGCATTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGTTCACGCTTACCTTGTAACGCAGAAAAAATTCTTGATTGCAAAATGAGTTGCTGCTTATCGTATTGTAACa                                                    <  1:850667/234‑1 (MQ=255)                                                                                                                                                                                      ttggggggaacctcccacaaTATTGGCTTCCCG‑TGGGG‑AA‑TTAAT‑GTGAGAAAATGTGC‑CAATGAAAATAAAAGTGGGTTTGT‑TTTTTTTGTTGTTTATA‑AAAAC‑GGGCGTACTTTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGTTCACGCTTACCTTGTAACGCAGAAAAAATTCTTGATTGCAAAATGAGTTGCTGCTTATCGTATTGTAACAAAt                                                 <  1:399756/237‑1 (MQ=255)                                                                                                                                                                                         ggagaaaccgcccaccaTATTGGCTCCCCG‑AGGGG‑AA‑TTATT‑GGGAATAAGTGTGA‑CAGTGAGAATTAAGGTG‑GGTTGATTTTTCTTGCT‑TTTTTA‑AAAATGGGGGGTGAATAAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGAt                                                                                                                                  <  1:39227/156‑1 (MQ=255)                                                                                                                                                                                                 cGTCCACCATATTGGCTTCCCC‑ATGGG‑AA‑TTAAT‑GTGAGTAAATGTGA‑CCATGATAATTAAAGTGCGGTTGA‑TTTTCTTGCTGTTTTTC‑AAAAT‑CGGCGTGCATTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGTTCACGCTTACCTTGTAACGCAGAAAAAATTCTTGATTGCAAAATGAGTTGCTGCTTATCGTATTGTAACAAATCGCGTAATTCa                                      >  1:731511/1‑251 (MQ=255)                                                                                                                                                                                                  gTCCACCATATTGGCTTCCCC‑ATGGG‑AA‑TTAAT‑GTGAGTAAATGTGA‑CCATGATAATTAAAGTGCGGTTGA‑TTTTCTTGCTGTTTTTC‑AAAAT‑CGGCGTGCATTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGTTCACGCTTACCTTGTAACGCAGAAAAAATTCTTGATTGCAAAATGAGTTGCTGCTTATCGTATTGTAACAAATCGCGTAATTc                                       >  1:387996/1‑249 (MQ=255)                                                                                                                                                                                                    ccaccaTATTGGCTTCCCC‑ATGGG‑AA‑TTAAT‑GTGAGTAAATGTGA‑CCATGATAATTAAAGTGCGGTTGA‑TTTTCTTGCTGTTTTTC‑AAAAT‑CGGCGTGCATTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGTTCACGCTTACCTTGTAACGCAGAAAAAATTCTTGATTGCAAAATGAGTTGCTGCTTATCGTATTGTaa                                                      >  1:165674/1‑232 (MQ=255)                                                                                                                                                                                                       ccaTATTGGCTTCCCC‑ATGGG‑AA‑TTAAT‑GTGAGTAAATGTGA‑CCATGATAATTAAAGTGCGGTTGA‑TTTTCTTGCTGTTTTTC‑AAAAT‑CGGCGTGCATTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGTTCACGCTTACCTTGTAACGCAGAAAAAATTCTTGATTGCAAAATGAGTTGCTGCTTATCGTATTGTAACAAAt                                                 >  1:299711/1‑234 (MQ=255)                                                                                                                                                                                                       ccaTATTGCCTCCCCC‑ATGGG‑AA‑TTAAT‑GTGAGTAAATGTGA‑CCATAATAATAAAAGTGCGTTTGT‑TTTTCTTGTTTTTTTTA‑AAAAT‑CGGCGTGCATTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGTTCACGCTTACCTTGTAACGCAGAAAAAATTCTTGATTGCAAAATGAGTTGCTGCTTATCGTATTGt                                                        <  1:201005/227‑1 (MQ=255)                                                                                                                                                                                                         atatTGGCTTCCCC‑ATGGG‑AA‑TTAAT‑GTGAGTAAATGTGA‑CCATGATAATTAAAGTGCGGTTGA‑TTTTCTTGCTGTTTTTC‑AAAAT‑CGGCGTGCATTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGTTCACGCTTACCTTGTAACGCAGAAAAAATTCTTGATTGCAAAATGAGTTGCTGCTTATCGTATTGTAAc                                                     >  1:527808/1‑228 (MQ=255)                                                                                                                                                                                                              ggCTTCCCC‑ATGGG‑AA‑TTAAT‑GTGAGTAAATGTGA‑CCATGATAATTAAAGTGCGGTTGA‑TTTTCTTGCTGTTTTTC‑AAAAT‑CGGCGTGCATTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGTTCACGCTTACCTTGTAACGCAGAAAAAATTCTTGATTGCAAAATGAGTTGCTGCTTATCGTATTGTAACAAATCGCGTAATTCAATGCCTTCCTCg                          >  1:733175/1‑250 (MQ=255)                                                                                                                                                                                                                     cc‑ATGGG‑AA‑TTAAT‑GTGAGTAAATGTGA‑CCATGATAATTAAAGTGCGGTTGA‑TTTTCTTGCTGTTTTTC‑AAAAT‑CGGCGTGCATTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGTTCACGCTTACCTTGTAACGCAGAAAAAATTCTTGATTGCAAAATGAGTTGCTGCTTATCGTATTGTAACAAAt                                                 >  1:55194/1‑220 (MQ=255)                                                                                                                                                                                                                           ggaaa‑TTTAT‑GTGATTAAATGTGC‑CCATTAAAATTAAGGTGCGTTTTT‑TTTTTTTCTTTTTTTTC‑AAAAT‑CGGCGTACATTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGTTCACGCTTACCTTGTAACGCAGAAAAAATTCTTGATTGCAAAATGAGTTGCTGCTTATCGTATTGTAACAAATCGCGTAATTCAATGCCTTCCTCGCCGAATAAACa               <  1:413606/247‑1 (MQ=255)                                                                                                                                                                                                                            g‑AA‑TTAAT‑GTGAGTAAATGTGA‑CCATGATAATTAAAGTGCGGTTGA‑TTTTCTTGCTGTTTTTC‑AAAAT‑CGGCGTGCATTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGcc                                                                                                                                             >  1:743942/1‑122 (MQ=255)                                                                                                                                                                                                                            g‑AA‑TTAAT‑GTGAGTAAATGTGA‑CCATGATAATTAAAGTGCGGTTGA‑TTTTCTTGCTGTTTTTC‑AAAAT‑CGGCGTGCATTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGTTCACGCTTACCTTGTAACGCAGAAAAAATTCTTGATTGCaaaa                                                                               >  1:429311/1‑184 (MQ=255)                                                                                                                                                                                                                            g‑AA‑TTAAT‑GTGAGTAAATGTGA‑CCATGATAATTAAAGTGCGGTTGA‑TTTTCTTGCTGTTTTTC‑AAAAT‑CGGCGTGCATTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGTTCACGCTTACCTTGTAACGCAGAAAAAATTCTTGATTGCAAAATGAGTTGCTGCTTATCGTATTGTAACAAATCGCGTAATTCAATGCCTTCCTCGCCGAATAAACAAAg            >  1:758091/1‑251 (MQ=255)                                                                                                                                                                                                                             aaa‑TTATT‑GTGAGTAAAGGTGA‑CCATGAAAATAAAGGGGCGTTTGT‑TTTTCTTG‑TTTTTTTA‑AAAATGTGGCGGATATAAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGTTCACGCTTACCTTGTAACGCAGAAAAAATTCTTGATTGCAAAATGAGTTGCTGCTTATCGTATTGTAACAAATCGCGTAATTCAATGCCTTCCTCGCCGAATAAACaaa             <  1:258517/249‑1 (MQ=255)                                                                                                                                                                                                                              aa‑TTAAT‑GTGAGTAAATGTGA‑CCATGATAATTAAAGTGCGGTTGA‑TTTTCTTGCTGTTTTTC‑AAAAT‑CGGCGTGCATTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGTTCACGCTTACCt                                                                                                              >  1:615531/1‑152 (MQ=255)                                                                                                                                                                                                                                    tt‑gtgATTAATTGTGA‑CCATGATAATAAAAGTGCGTTTGT‑TTTTTTTGTTTTTTTTA‑AAAAT‑CGGCGTGCATTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGTTCACGCTTACCTTGTAACGCAGAAAAAATTCTTGATTGCAAAATGAGTTGCTGCTTATCGTATTGTAACAAAt                                                 <  1:362336/207‑1 (MQ=255)                                                                                                                                                                                                                                    aT‑GTGAGTAAATGTGA‑CCATGATAATTAAAGTGCGGTTGA‑TTTTCTTGCTGTTTTTC‑AAAAT‑CGGCGTGCATTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGTTCACGCTTACCTTGTAACGCAGAAAAAATTCTTGATTGCAAAATGAGTTGCTGCTTATCGTATTGTAACAAATCGCGTAATTCAATGCCTTCCTCGCCGAATAAACAAAGGTGCaa      >  1:511733/1‑251 (MQ=255)                                                                                                                                                                                                                                         gAGTAAATGTGA‑CCATGATAATTAAAGTGCGGTTGA‑TTTTCTTGCTGTTTTTC‑AAAAT‑CGGCGTGCATTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGTTCACGCTTACCTTGTAACGCAGAAAAAATTCTTGATTGCAAAATGAGTTGCTGCTTATCGTATTGTAACAAATCGCGTAAt                                         >  1:182124/1‑212 (MQ=255)                                                                                                                                                                                                                                         gAGTAAATGTGA‑CCATGATAATTAAAGTGCGGTTGA‑TTTTCTTGCTGTTTTTC‑AAAAT‑CGGCGTGCATTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGTTCACGCTTACCTTGTAACGCAGAAAAAATTCTTGATTGCAAAATGAGTTGCTGCTTATCGTATTGTAACAAATCGCGTAATTCAATGc                                  >  1:372700/1‑219 (MQ=255)                                                                                                                                                                                                                                         gAGTAAATGTGA‑CCATGATAATTAAAGTGCGGTTGA‑TTTTCTTGCTGTTTTTC‑AAAAT‑CGGCGTGCATTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGTTCACGCTTACCTTGTAACGCAGAAAAAATTCTTGATTGCAAAATGAGTTGCTGCTTATCGTATTGTAACAAATCGCGTAATTCAATGCCTTCCTCGCCGAATAAACAAAGGTGCAAttt   >  1:27230/1‑250 (MQ=255)                                                                                                                                                                                                                                         gAGTAAATGTGA‑CCATGATAATTAAAGTGCGGTTGA‑TTTTCTTGCTGTTTTTC‑AAAAT‑CGGCGTGCATTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGTTCACGCTTACCTTGTAACGCAGAAAAAATTCTTGATTGCAAAATGAGTTGCTGCTTATCGTATTGTAACAAATCGCGTAATTCAATGCCTTCCTCGCCGAATAAACAAAGGTGCAAttt   >  1:749994/1‑250 (MQ=255)                                                                                                                                                                                                                                         aataaaaTGTGA‑CCATTATAATAAAGGTGCGGTTGT‑TTTTTTTCCTTTTTTAC‑AAAAC‑CGGCGTGCATTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGTTCACGCTTACCTTGTAACGCAGAAAAAATTCTTGATTGCAAAATGAGTTGCTGCTTATCGTATTGTAACAAATCGCGTAATTCAATGCCTTCCTCGCCGAATAAACAAAGGTGCAATTTc  <  1:94136/247‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                    AAGCAATAACGGATGGCACAAGGGAATCAATTCCCACGTGCGTTTTGCCGCCTGAATGCCGGCAATGCGGGCGGTGGCGAACACATCGCCTTTGTGATGCTGACCGGACAGGATCATTTGTAAGGTTTCGGGCGCCATACAAACATAAGCCTCAGCGCGGGCTTCACGGACGGTTTCGGCTTT‑GGCGGAAACGTCCACCATATTGGCTTCCCC‑ATGGG‑AA‑TTAAT‑GTGAGTAAATGTGG‑CCATGATAATTAAAGTGCGGTTGA‑TTTTCTTGCTGTTTTTC‑AAAAT‑CGGCGTGCATTAACCGCCGATACTTGCCAAATGACTACGTACGCCACTATCGCCGATGTGCAGATAATGATGTTCACGCTTACCTTGTAACGCAGAAAAAATTCTTGATTGCAAAATGAGTTGCTGCTTATCGTATTGTAACAAATCGCGTAATTCAATGCCTTCCTCGCCGAATAAACAAAGGTGCAATTTC  >  1/1157841‑1158318

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑