Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,155,198	C→T	G394G (GGC→GGT)	unknown →	Asparaginyl‑tRNA synthetase (EC 6.1.1.22)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,155,198	0	C	T	100.0%	177.8 / NA	66	G394G (GGC→GGT)	unknown	Asparaginyl‑tRNA synthetase (EC 6.1.1.22)
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (28/38);  total (28/38)

GGATTACACCGACGCCATTGAGATTTTACTGAAATCCGGTAAAACCTTTGAATTCCCGGTTTCTTGGGGCATTGACCTTTCCTCCGAACATGAGCGCTATTTGGCAGAAGATTATTTCAAATCCCCTGTTGTGGTGAAAAACTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGCTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGACAAACGCATGGCGGAAATGGGGCTAAATCCGGAAGATTATTGGTGGTATCGTGATTTGCGTCGTTACGGCACGGTTCCGCATTCCGGCTTTGGCTTGGGCTTTGAACGTTTAATTGTTTATGTGACAGGGTTGCAAAATATTCGTGATGTGATCCCGTTCCCACGTGCTCCGCGTAACGCAAATTTCTAATTC  >  1/1154952‑1155423                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                   ggATTACACCGACGCCATTGAGATTTTACTGAAATCCGGTAAAACCTTTGAATTCCCGGTTTCTTGGGGCATTGACCTTTCCTCCGAACATGAGCGCTATTTGGCAGAAGATTATTTCAAATCCCCTGTTGTGGTGAAAAACTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCg                                                                                                                                                                                                                                 >  1:766798/1‑250 (MQ=255) ggATTACACCGACGCCATTGAGATTTTACTGAAATCCGGTAAAACCTTTGAATTCCCGGTTTCTTGGGGCATTGACCTTTCCTCCGAACATGAGCGCTATTTGGCAGAAGATTATTTCAAATCCCCTGTTGTGGTGAAAAACTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCg                                                                                                                                                                                                                                 >  1:412460/1‑250 (MQ=255)       cacCGACGCCATTGAGATTTTACTGAAATCCGGTAAAACCTTTGAATTCCCGGTTTCTTGGGGCATTGACCTTTCCTCCGAACATGAGCGCTATTTGGCAGAAGATTATTTCAAATCCCCTGTTGTGGTGAAAAACTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTg                                                                                                                                                                                                                          >  1:387193/1‑251 (MQ=255)              gCCATTGAGATTTTACTGAAATCCGGTAAAACCTTTGAATTCCCGGTTTCTTGGGGCATTGACCTTTCCTCCGAACATGAGCGCTATTTGGCAGAAGATTATTTCAAATCCCCTGTTGTGGTGAAAAACTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑Cg                                                                                                                                                                                                                  >  1:400219/1‑251 (MQ=255)                       aTTTTACTGAAATCCGGTAAAACCTTTGAATTCCCGGTTTCTTGGGGCATTGACCTTTCCTCCGAACATGAGCGCTATTTGGCAGAAGATTATTTCAAATCCCCTGTTGTGGTGAAAAACTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACg                                                                                                                                                                                                          <  1:182051/250‑1 (MQ=255)                       aTTTTACTGAAATCCGGTAAAACCTTTGAATTCCCGGTTTCTTGGGGCATTGACCTTTCCTCCGAACATGAGCGCTATTTGGCAGAAGATTATTTCAAATCCCCTGTTGTGGTGAAAAACTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACg                                                                                                                                                                                                          <  1:423466/250‑1 (MQ=255)                       aTTTTACTGAAATCCGGTAAAACCTTTGAATTCCCGGTTTCTTGGGGCATTGACCTTTCCTCCGAACATGAGCGCTATTTGGCAGAAGATTATTTCAAATCCCCTGTTGTGGTGAAAAACTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACg                                                                                                                                                                                                          <  1:467169/250‑1 (MQ=255)                            aCTGAAATCCGGTAAAACCTTTGAATTCCCGGTTTCTTGGGGCATTGACCTTTCCTCCGAACATGAGCGCTATTTGGCAGAAGATTATTTCAAATCCCCTGTTGTGGTGAAAAACTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGTATTAg                                                                                                                                                                                                    >  1:616189/1‑251 (MQ=255)                              tGAAATCCGGTAAAACCTTTGAATTCCCGGTTTCTTGGGGCATTGACCTTTCCTCCGAACATGAGCGCTATTTGGCAGAAGATTATTTCAAATCCCCTGTTGTGGTGAAAAACTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGAc                                                                                                                                                                                                  <  1:110582/251‑1 (MQ=255)                              tGAAATCCGGTAAAACCTTTGAATTCCCGGTTTCTTGGGGCATTGACCTTTCCTCCGAACATGAGCGCTATTTGGCAGAAGATTATTTCAAATCCCCTGTTGTGGTGAAAAACTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGAc                                                                                                                                                                                                  <  1:220519/251‑1 (MQ=255)                              tGAAATCCGGTAAAACCTTTGAATTCCCGGTTTCTTGGGGCATTGACCTTTCCTCCGAACATGAGCGCTATTTGGCAGAAGATTATTTCAAATCCCCTGTTGTGGTGAAAAACTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGAc                                                                                                                                                                                                  <  1:487137/251‑1 (MQ=255)                                    ccGGTAAAACCTTTGAATTCCCGGTTTCTTGGGGCATTGACCTTTCCTCCGAACATGAGCGCTATTTGGCAGAAGATTATTTCAAATCCCCTGTTGTGGTGAAAAACTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGACAAACGc                                                                                                                                                                                            <  1:149162/251‑1 (MQ=255)                                      ggTAAAACCTTTGAATTCCCGGTTTCTTGGGGCATTGACCTTTCCTCCGAACATGAGCGCTATTTGGCAGAAGATTATTTCAAATCCCCTGTTGTGGTGAAAAACTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGACAAACGCAt                                                                                                                                                                                          >  1:272469/1‑251 (MQ=255)                                      ggTAAAACCTTTGAATTCCCGGTTTCTTGGGGCATTGACCTTTCATCCGAACATGAGCGCTATTTGGCAGAAGATTATTTCAAATCCCCTGTTGTGGTGAAAAACTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAAAGGGAAGAA‑AGCTTAGACGTATTAGACAAAAGCAt                                                                                                                                                                                          >  1:447847/1‑251 (MQ=255)                                          aaaCCTTTGAATTCCCGGTTTCTTGGGGCATTGACCTTTCCTCCGAACATGAGCGCTATTTGGCAGAAGATTATTTCAAATCCCCTGTTGTGGTGAAAAACTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGACAAACGCATGGCg                                                                                                                                                                                      <  1:829387/251‑1 (MQ=255)                                            aCCTTTGAATTCCCGGTTTCTTGGGGCATTGACCTTTCCTCCGAACATGAGCGCTATTTGGCAGAAGATTATTTCAAATCCCCTGTTGTGGTGAAAAACTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGAc                                                                                                                                                                                                           <  1:119702/228‑1 (MQ=255)                                                           gTTTCTTGGGGCATTGACCTTTCCTCCGAACATGAGCGCTATTTGGCAGAAGATTATTTCAAATCCCCTGTTGTGGTGAAAAACTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACCTGAAGAA‑CGTTTAGACGTATTAGACAAACGCATGGCGGAAATGGGGCTAAATc                                                                                                                                                                      >  1:154598/1‑250 (MQ=255)                                                             ttCTTGGGGCATTGACCTTTCCTCCGAACATGAGCGCTATTTGGCAGAAGATTATTTCAAATCCCCTGTTGTGGTGAAAAACTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGACAAAAGCATGGCGGGAATGGGGCTAAATCCgg                                                                                                                                                                   >  1:110777/1‑251 (MQ=255)                                                                    ggCATTGACCTTTCCTCCGAACATGAGCGCTATTTGGCAGAAGATTATTTCAAATCCCCTGTTGTGGTGAAAAACTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGGGAAGAA‑CGTTTAGACGGATTAGACAAACGCATGGCGGAAATGGGGCTAAATCCGGAAGatta                                                                                                                                                            >  1:807123/1‑251 (MQ=255)                                                                        ttGACCTTTCCTCCGAACATGAGCGCTATTTGGCAGAAGATTATTTCAAATCCCCTGTTGTGGTGAAAAACTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGACAAACGCATGGCGGAAATGGGGCTAAATCCGGAAGATTATtgg                                                                                                                                                        <  1:282093/251‑1 (MQ=255)                                                                         tGACCTTTCCTCCGAACATGAGCGCTATTTGGCAGAAGATTATTTCAAATCCCCTGTTGTGGTGAAAAACTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGACAAACGCATGGCGGAAATGGGGc                                                                                                                                                                            <  1:343846/230‑1 (MQ=255)                                                                         tGACCTTTCCTCCGAACATGAGCGCTATTTGGCAGAAGATTATTTCAAATCCCCTGTTGTGGTGAAAAACTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGACAAACGCATGGCGGAAATGGGGCTAAATCCGGAAGATTATtggt                                                                                                                                                       <  1:377910/251‑1 (MQ=255)                                                                         tGACCTTTCCTCCGAACATGAGCGCTATTTGGCAGAAGATTATTTCAAATCCCCTGTTGTGGTGAAAAACTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGACAAACGCATGGCGGAAATGGGGCTAAATCCGGAAGATTATtggt                                                                                                                                                       <  1:454650/251‑1 (MQ=255)                                                                            ccTTTCCTCCGAACATGAGCGCTATTTGGCAGAAGATTATTTCAAATCCCCTGTTGTGGTGAAAAACTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGACAAACGCATGGCGGAAATGGGGCTAAATCCGGAAGATTATTggtggt                                                                                                                                                    <  1:467811/251‑1 (MQ=255)                                                                               ttCCTCCGAACATGAGCGCTATTTGGCAGAAGATTATTTCAAATCCCCTGTTGTGGTGAAAAACTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAAAGTGAAAAACCGTTT‑GACGTCTTATACAAACACAAGGCGGAAATGGGGCTAAATCCGGGAGattatttggtgtct                                                                                                                                                  >  1:538120/1‑242 (MQ=255)                                                                                         cATGAGCGCTATTTGGCAGAAGATTATTTCAAATCCCCTGTTGTGGTGAAAAACTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTTGCCACGGGGAGAA‑CGTTTTGACGTATTAGACAAAAGCATGGCGGAAATGGGGCTAAATCCGGGAGATTATTTGTGGTATCGTGATTTGcg                                                                                                                                       >  1:422671/1‑251 (MQ=255)                                                                                              gcgcTATTTGGCAGAAGATTATTTCAAATCCCCTGTTGTGGTGAAAAACTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGACAAACGCATGGCGGAAAAGGGGCTAAATCCGGGAGATTATTGGTGGGATCGTGATTTGCgttgtt                                                                                                                                  >  1:251019/1‑251 (MQ=255)                                                                                                 cTATTTGGCAGAAGATTATTTCAAATCCCCTGTTGTGGTGAAAAACTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGACAAACGCATGGCGGAAATGGGGCTAAATCCGGAAGATTATTGGTGGTATCGTGATTTGCGTTGTTAc                                                                                                                                <  1:561997/250‑1 (MQ=255)                                                                                                         cAGAAGATTATTTCAAATCCCCTGTTGTGGTGAAAAACTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGACAAACGCATGGCGGAAATGGGGCTAAATCCGGAAGATTATTGGTGGTATCGTGATTTGCGTTGTTACGGCACGGt                                                                                                                        <  1:175573/250‑1 (MQ=255)                                                                                                             agaTTATTTCAAATCCCCTGTTGTGGTGAAAAACTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCCACGTGAAGAA‑AGTTTTGACGGATTTGACAAACGCATGGCGGCAATGGGGCTAAATTCGGAAGATTATTTGTGGGATCGTGATTTGCGTTGTTTCGGCCCGGCTCCg                                                                                                                    >  1:394106/1‑250 (MQ=255)                                                                                                              gaTTATTTCAAATCCCCTGTTGTGGTGAAAAACTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGGATTAGACAAACGCATGGCGGGAATGGGGCTAAATCCGGAAGATTATTGGTGGTATCGGGATTTGCGGTGTTACGGCACGGGTCCGCa                                                                                                                  >  1:209503/1‑251 (MQ=255)                                                                                                                   tttCAAATCCCCTGTTGTGGTGAAAAACTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAAAGTGAAGAA‑CGTTTAGACGTATTAGACAAACGCATGGCGGAAATGGGGCTAAATCCGGAAGATTATTGGTGGTATCGTGATTTGCGTTGTTACGGCACGGGTCCGCATTCCg                                                                                                             >  1:719648/1‑251 (MQ=255)                                                                                                                     tCAAATCCCCTGTTGTGGTGAAAAACTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGACAAACGCATGGCGGAAATGGGGCTAAATCCGGAAGATTATTGGTGGTATCGTGATTTGCGTTGTTACGGCACGGTTCCGCATTCCGGc                                                                                                           <  1:229881/251‑1 (MQ=255)                                                                                                                           ccccTGTTGTGGTGAAAAACTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGAc                                                                                                                                                                                                  <  1:6257/158‑1 (MQ=255)                                                                                                                           ccccTGTTGTGGTGAAAAACTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGACAAACGCATGGCGGAAATGGGGCTAAATCCGGAAGATTATTGGTGGTATCGTGATTTGCGTTGTTACGGCACGGTTCCGCATTCCGGCTTTGGc                                                                                                     <  1:150810/251‑1 (MQ=255)                                                                                                                              cTGTTGTGGTGAAAAACTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGACAAACGCATGGCGGAAATgg                                                                                                                                                                               >  1:124692/1‑174 (MQ=255)                                                                                                                                gttgtGGTGAAAAACTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGGGAAGAA‑CGTTTAGACGTATTAGACAAACGCATGGCGGGAATGGGGCTAAATCCGGGAGATTATTTGTGGGATCGTGGTTTGCGTTGGTACGGCACGGTTCCGCATTCCGGCTTTTGCTTggg                                                                                                >  1:745855/1‑251 (MQ=255)                                                                                                                                     ggtgAAAAACTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGACAAACGCATGGCGGAAATGGGGCTAAATCCGGAAGATTATTGGTGGTATCGTGATTTGCGTTGTTACGGCACGGTTCCGCAt                                                                                                                 <  1:37358/229‑1 (MQ=255)                                                                                                                                     ggtgAAAAACTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGGTTAGACGTATTAGACAAACGCATGGCGGGAATGGGGCTAAATCCGGAAGATTATTGGGGGTATCGTGATTTGCGTTGTTACGGCACGGGTCCGCATTCCGGCTTTGGCTTGGGCtttt                                                                                           >  1:418037/1‑250 (MQ=255)                                                                                                                                      gtgAAAAACTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGACAAACGCATGGCGGAAATGGGGCTAAATCCGGAAGATTATTGGTGGTATCGTGATTTGCGTTGTTACGGCACGGTTCCGCAt                                                                                                                 <  1:646891/228‑1 (MQ=255)                                                                                                                                          aaaaCTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGCGAAGAA‑CGTTTAGACGTATTAGACAAACGCATGGCGGAAATGGGGCTAAATCCCGGAGATTTTTTGTGGTATCGTGATTTGCGTTGTTACGGCACGGTTCCGCATTCCGGCTTTTGCTTTGGCTTTGAAc                                                                                        >  1:716542/1‑249 (MQ=255)                                                                                                                                                 tCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGACAAACGCATGGCGGAAATGGGGCTAAATCCGGAAGATTATTGGTGGTATCGTGATTTGCGTTGTTACGGCACGGTTCCGCATTCCGGCTTTGGCTTGGGCTTTGAACGTTTAATTg                                                                               <  1:670036/251‑1 (MQ=255)                                                                                                                                                 tCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGACAAACGCATGGCGGAAATGGGGCTAAATCCGGAAGATTATTGGTGGTATCGTGATTTGCGTTGTTACGGCACGGTTCCGCATTCCGGCTTTGGCTTGGGCTTTGAACGTTTAATTg                                                                               <  1:346762/251‑1 (MQ=255)                                                                                                                                                         aaaTTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGACAAACGCATGGCGGAAATGGGGCTAAATCCGGAAGATTATTGGTGGTATCGTGATTTGCGTTGTTACGGCACGGTTCCGCATTCCGGCTTTGGCTTGGGCTTTGAACGTTTAATTGTTTAtgtg                                                                       <  1:195012/251‑1 (MQ=255)                                                                                                                                                         aaaTTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGACAAACGCATGGCGGAAATGGGGCTAAATCCGGAAGATTATTGGTGGTATCGTGATTTGCGTTGTTACGGCACGGTTCCGCATTCCGGCTTTGGCTTGGGCTTTGAACGTTTAATTGTTTAtgtg                                                                       <  1:397338/251‑1 (MQ=255)                                                                                                                                                           aTTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCCACGTGAAGAA‑CGTTTAGACGGATTAGACAAACGCATGGCGGAAATGGGGCTAAATCCGGAAGATTATTGGGAGGATCGTGATTTGCGGTGTTACGGCCCGGGTCCGCATTCCGGCTTTGGCTTGGGCTTTGAACGTTTAATTGTTTTTGTGa                                                                      >  1:426404/1‑250 (MQ=255)                                                                                                                                                           aTTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGACAAACGCATGGCGGAAATGGGGCTAAATCCGGAAGATTATTGGTGGTATCGTGATTTGCGTTGTTACGGCACGGTTCCGCAt                                                                                                                 <  1:648823/207‑1 (MQ=255)                                                                                                                                                           aTTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGACAAACGCATGGCGGAAATGGGGCTAAATCCGGAAGATTATTGGTGGTATCGTGATTTGCGTTGTTACGGCACGGTTCCGCATTCCGGCTTTGGCTTGGGCTTTGAACGTTTAATTGTTTATGTGAc                                                                     <  1:545296/251‑1 (MQ=255)                                                                                                                                                                  ccTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGACAAACGCATGGCGGAAATGGGGCTAAATCCGGAAGATTATTGGTGGTATCGTGATTTGCGTTGTTACGGCACGGTTCCGCATTCCGGCTTTGGCTTGGGCTTTGAACGTTTAATTGTTTATGTGACAGGGt                                                                <  1:818436/249‑1 (MQ=255)                                                                                                                                                                    ttCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGACAAACGCATGGCGGAAATGGGGCTAAATCCGGAAGATTATtggtg                                                                                                                                                      <  1:44866/161‑1 (MQ=255)                                                                                                                                                                    ttCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGGATTAGACAAACGCATGGCGGAAATGGGGCTAAATCCGGAAGATTATTGGTGGTATCGTGATTTGCGTTGTTACGGCACGGTTCCGCATTCCGGCTTTTGCTTGGGCTTTTAACGTTTAATTTTTTTTGTGACAGGGTTGCa                                                            >  1:91117/1‑251 (MQ=255)                                                                                                                                                                    ttCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGGATTAGACAAACGCATGGCGGAAATGGGGCTAAATCCGGAAGATTATTGGTGGTATCGTGATTTGCGTTGTTACGGCACGGGTCCGCATTCCGGCTTTGGCTTGGGCTTTGAACGTTTAATTg                                                                               >  1:872604/1‑232 (MQ=255)                                                                                                                                                                     tCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGACAAACGCATGGCGGAAAAGGGGCTAAATCCGGGAGATTATTGGTGGTATCGTGa                                                                                                                                             >  1:335935/1‑169 (MQ=255)                                                                                                                                                                      cTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGACAAACGCATGGCGGAAATGGGGCTAAATCCGGAAGATTATTGGTGGTATCGTGATTTGCGTTGTTACGGCACGGTTCCGCATTCCGGCTTTGGCTTGGGCTTTGaa                                                                                         <  1:639203/220‑1 (MQ=255)                                                                                                                                                                               cTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGACAAACGCATGGCGGAAATGGGGCTAAATCCGGAAGATTATTGGTGGTATCGTGATTTGCGTTGTTACGGCACGGTTCCGCATTCCGGCTTTGGCTTGGGCTTTGAACGTTTAATTGTTTATGTGACAGGGTTGCAAAATATTCgtg                                                 <  1:476432/251‑1 (MQ=255)                                                                                                                                                                               cTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGACAAACGCATGGCGGAAATGGGGCTAAATCCGGAAGATTATTGGTGGTATCGTGATTTGCGTTGTTACGGCACGGTTCCGCATTCCGGCTTTGGCTTGGGCTTTGAACGTTTAATTGTTTATGTGACAGGGTTGCAAAATATTCgtg                                                 <  1:355217/251‑1 (MQ=255)                                                                                                                                                                                            ggACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGGATTAGACAAAAGCATGGCGGGAATGGGGCCAAATCCGGAAGATTATTGGGGGGATCGTGATTTTCGTTGTTATGGCACGGGTCCGCATTTCGGCTTTGGCTTGGGGTTTTTAAGTTTAATTGTTTTTGTGACAGGGGTGCAAAATATTCGGGATGTGAACCCGtt                                    >  1:320376/1‑251 (MQ=255)                                                                                                                                                                                             gACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGACAAACGCATGGCGGAAATGGGGCTAAATCCGGAAGATTATTGGGGGGATCGTGGTTTGCGTTGTTACGGCACGGGTCCGCATTCCGGCTTTGGCTTGGGCTTTTAACGGTTAATTGTTTATGTGACAGGGTTTCAAAAAATTCgtg                                                 >  1:387449/1‑237 (MQ=255)                                                                                                                                                                                             gACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGGATTAGACAAACGCATGGCGGGAATGGGGCTAAATCCGGGAGATTATTGGTGGGATCGTGATTTGCGTTGTTACGGCACGGGTCCGCATTCCGGCTTTGGCTTGGGCTTTGAACGTTTAATTGTTTATGTGACAGGGGTGCAAAATATTCGTGATGTGATcc                                        >  1:15484/1‑246 (MQ=255)                                                                                                                                                                                                  aCCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGACAAACGCATGGCGGAAATGGGGCTAAATCCGGAAGATTATTGGTGGTATCGTGATTTGCGTTGTTACGGCACGGTTCCGCATTCCGGCTTTGGCTTGGGCTTTGAACGTTTAATTGTTTATGTGACAGGGTTGCAAAATATTCGTGATGTGATCCCGTTCCCACg                              <  1:197478/251‑1 (MQ=255)                                                                                                                                                                                                       tGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGACAAACGCATGGCGGAAATGGGGCTAAATCCGGAAGATTATTGGTGGTATCGTGATTTGCGTTGTTACGGCACGGTTCCGCATTCCGGCTTTGGCTTGGGCTTTGAACGTTTAATTGTTTATGTGACAGGGTTGCAAAATATTCGTGATGTGATCCCGTTCCCACGTGCTc                         <  1:157029/251‑1 (MQ=255)                                                                                                                                                                                                                                    ttGGTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGACAAACGCATGGCGGAAATGGGGCTAAATCCGGAAGATTATTGGTGGTATCGTGATTTGCGTTGTTACGGCACGGTTCCGCATTCCGGCTTTGGCTTGGGCTTTGAACGTTTAATTGTTTATGTGACAGGGTTGCAAAATATTCGTGATGTGATcc                                        <  1:11730/207‑1 (MQ=255)                                                                                                                                                                                                                                      ggTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGACAAACGCATGGCGGGAATGGGGCTAAATCCGGGAGATTATTGGTGGTATCGTGATTTGCGGTGTTACGGCACGGGTCCGCCTTCCGGCTTTGGCTTGGGGTTTGAACGTTTAATTGTTTATGTGACAGGGGTGCAAAATATTCGTGATGTGATCCCGTTCCCACGTGCTc                         >  1:390489/1‑220 (MQ=255)                                                                                                                                                                                                                                      ggTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGACAAACGCATGGCGGAAATGGGGCTAAATCCGGAAGATTATTGGTGGTATCGTGATTTGCGTTGTTACGGCACGGTTCCGCATTCCGGCTTTGGCTTGGGCTTTGAACGTTTAATTGTTTATGTGACAGGGTTGCAAAATATTCGTGATGTGATCCCGTTCCCACGTGCTCCGCGTAACGCAAAttt         <  1:806297/236‑1 (MQ=255)                                                                                                                                                                                                                                       gTGAAATTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGACAAACGCATGGCGGAAATGGGGCTAAATCCGGAAGATTATTGGTGGTATCGTGATTTGCGTTGTTACGGCACGGTTCCGCATTCCGGCTTTGGCTTGGGCTTTGAACGTTTAATTGTTTATGTGACAGGGTTGCAAAATATTCGTGATGTGATcc                                        <  1:142697/204‑1 (MQ=255)                                                                                                                                                                                                                                            aTTATCGGCGGTTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGACAAACGCATGGCGGAAATGGGGCTAAATCCGGAAGATTATTGGTGGTATCGTGATTTGCGTTGTTACGGCACGGTTCCGCATTCCGGCTTTGGCTTGGGCTTTGAACGTTTAATTGTTTATGTGACAGGGTTGCAAAATATTCGTGATGTGATCCCGTTCCCACGTGCTCCGCGTAACGCAAATTTCTAATtc  <  1:581780/237‑1 (MQ=255)                                                                                                                                                                                                                                                       |                                                                                                                                                                                                                                   GGATTACACCGACGCCATTGAGATTTTACTGAAATCCGGTAAAACCTTTGAATTCCCGGTTTCTTGGGGCATTGACCTTTCCTCCGAACATGAGCGCTATTTGGCAGAAGATTATTTCAAATCCCCTGTTGTGGTGAAAAACTATCCGAAAGAAATTAAAGCCTTCTATATGCGCTTAAATGACGATGGACAAACCGTTGCGGCAATGGATGTTTTAGCGCCGGGCATTGGTGAAATTATCGGCGGCTCGCAACGTGAAGAA‑CGTTTAGACGTATTAGACAAACGCATGGCGGAAATGGGGCTAAATCCGGAAGATTATTGGTGGTATCGTGATTTGCGTCGTTACGGCACGGTTCCGCATTCCGGCTTTGGCTTGGGCTTTGAACGTTTAATTGTTTATGTGACAGGGTTGCAAAATATTCGTGATGTGATCCCGTTCCCACGTGCTCCGCGTAACGCAAATTTCTAATTC  >  1/1154952‑1155423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑