| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | 1 | 1,154,248 | A→G | T78A (ACC→GCC) | unknown → | Asparaginyl‑tRNA synthetase (EC 6.1.1.22) |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | 1 | 1,154,248 | 0 | A | G | 98.2% | 129.5 / ‑5.8 | 55 | T78A (ACC→GCC) | unknown | Asparaginyl‑tRNA synthetase (EC 6.1.1.22) |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base G (27/27); minor base C (1/0); total (28/27) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.42e-01 | |||||||||||
GGTATATATGACAAAAATTGCATCAATTGTAAATGTGCTGCAAGGCAAAATTGCAATTGGCGAAAAAGTGACTGTGCGCGGCTGGGTGCGCACCCGTCGCGACTCCAAAGCAGGCCTATCCTTTCTTGCCGTTTATGACGGTTCTTGCTTCGCACCCATTCAAGCTATTGTAAATAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGTTGTTCCGTGGTTGTCACCGGCACCGTAGTCGAATCGCCGGCAGAAGGTCAGGCAGTAGAATTACAAGCGGAAAAAGTGGAAGTCACCGGTTGGGTAGAAGATCCGGAAACTTACCCGATGGCGGCAAAACGTCACTCTATCGAATATTTACGCGAAGTGGCGCACCTACGCCCGCGCACCAATATTATCGGTGCAGTTGCCCGTGTTCGTCATTGCTTGGCACAAGCCATTCATCGTTTCTTTCATGAACAAGGT > 1/1154010‑1154487 | ggTATATATGACAAAAATTGCATCAATTGTAAATGTGCTGCAAGGCAAAATTGCAATTGGCGAAAAAGTGACTGTGCGCGGCTGGGTGCGCACCCGTCGCGACTCCAAAGCAGGCCTATCCTTTCTTGCCGTTTATGACGGTTCTTGCTTCGCACCCATTCAAGCTATTGTAAATAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGTTGTTCCGTGGTTGTCGCCGGCACCCTAg > 1:392147/1‑251 (MQ=255)ggTATATATGACAAAAATTGCATCAATTGTAAATGTGCTGCAAGGCAAAATTGCAATTGGCGAAAAAGTGACTGTGCGCGGCTGGGTGCGCACCCGTCGCGACTCCAAAGCAGGCCTATCCTTTCTTGCCGTTTATGACGGTTCTTGCTTCGCACCCATTCAAGCTATTGTAAATAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGGTGTTCCGTGGGTGTCGCCCGCACCGTAg > 1:465781/1‑251 (MQ=255) atatatGACAAAAATTGCATCAATTGTAAATGTGCTGCAAGGCAAAATTGCAATTGGCGAAAAAGTGACTGTGCGCGGCTGGGTGCGCACCCGTCGCGACTCCAAAGCAGGCCTATCCTTTCTTGCCGTTTATGACGGTTCTTGCTTCGCACCCATTCAAGCTATTGTAAATAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGTTGTTCCGTGGTTGTCGCCGGCCCCGTAGTCg > 1:38355/1‑251 (MQ=255) atatatGACAAAAATTGCATCAATTGTAAATGTGCTGCAAGGCAAAATTGCAATTGGCGAAAAAGTGACTGTGCGCGGCTGGGTGCGCACCCGTCGCGACTCCAAAGCAGGCCTATCCTTTCTTGCCGTTTATGACGGTTCTTGCTTCGCACCCATTCAAGCTATTGTAAATAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGGTGTTCCGGGGGTGTCGCCGGCCCCGTAGTCg > 1:661008/1‑251 (MQ=255) atGACAAAAATTGCATCAATTGTAAATGTGCTGCAAGGCAAAATTGCAATTGGCGAAAAAGTGACTGTGCGCGGCTGGGTGCGCACCCGTCGCGACTCCAAAGCAGGCCTATCCTTTCTTGCCGTTTATGACGGTTCTTGCTTCGCACCCATTCAAGCTATTGTAAATAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGTTGTTCCGTGGTTGTCGCCGGCACCGTAGTCGAATc < 1:236849/251‑1 (MQ=255) aTTGTAAATGTGCTGCAAGGCAAAATTGCAATTGGCGAAAAAGTGACTGTGCGCGGCTGGGTGCGCACCCGTCGCGACTCCAAAGCAGGCCTATCCTTTCTTGCCGTTTATGACGGTTCTTGCTTCGCACCCATTCAAGCTATTGTAAATAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGTTGTTCCGTGGTTGTCGCCGGCACCGTAGTCGAATCGCCGGCAGAAGGGCAggg > 1:481043/1‑250 (MQ=255) aTTGTAAATGTGCTGCAAGGCAAAATTGCAATTGGCGAAAAAGTGACTGTGCGCGGCTGGGTGCGCACCCGTCGCGACTCCAAAGCAGGCCTATCCTTTCTTGCCGTTTATGACGGTTCTTGCTTCGCACCCATTCAAGCTATTGTAAATAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGTTGTTCCCTGGTTGTCGCCGGCACCCTAGTCGAATCGCCGGCCGAAGGTCAgg > 1:877566/1‑250 (MQ=255) aTTGTAAATGTGCTGCAAGGCAAAATTGCAATTGGCGAAAAAGTGACTGTGCGCGGCTGGGTGCGCACCCGTCGCGACTCCAAAGCAGGCCTATCCTTTCTTGCCGTTTATGACGGTTCTTGCTTCGCACCCATTCAAGCTATTGTAAATAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGGTGTTTCGTGGTTGTCGCCGGCACCGTAGTCGAATCGCCGGCAGAAGGGCAggg > 1:598073/1‑250 (MQ=255) ttGTAAATGTGCTGCAAGGCAAAATTGCAATTGGCGAAAAAGTGACTGTGCGCGGCTGGGTGCGCACCCGTCGCGACTCCAAAGCAGGCCTATCCTTTCTTGCCGTTTATGACGGTTCTTGCTTCGCACCCATTCAAGCTATTGTAAATAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGGTGTTCCGTGGTTGTCGCCCGCCCCGTAGTCGAATCGCCGGCAGGAGGTCCGGcg > 1:269176/1‑250 (MQ=255) tgtgCTGCAAGGCAAAATTGCAATTGGCGAAAAAGTGACTGTGCGCGGCTGGGTGCGCACCCGTCGCGACTCCAAAGCAGGCCTATCCTTTCTTGCCGTTTATGACGGTTCTTGCTTCGCACCCATTCAAGCTATTGTAAATAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGTTGTTCCGTGGTTGTCGCCGGCACCGTAGTCGAATCGCCGGCAGAAGGTCAGGCAGTAGAAt < 1:387583/251‑1 (MQ=255) tgctgcAAGGCAAAATTGCAATTGGCGAAAAAGTGACTGTGCGCGGCTGGGTGCGCACCCGTCGCGACTCCAAAGCAGGCCTATCCTTTCTTGCCGTTTATGACGGTTCTTGCTTCGCACCCATTCAAGCTATTGTAAATAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGTTGTTCCGTGGTTGTCGCCGGCACCGTAGTCGAATCGCCGGCAGAAGGTCAGGCAGTAGAATTa < 1:785014/251‑1 (MQ=255) ctgcAAGGCAAAATTGCAATTGGCGAAAAAGTGACTGTGCGCGGCTGGGTGCGCACCCGTCGCGACTCCAAAGCAGGCCTATCCTTTCTTGCCGTTTATGACGGTTCTTGCTTCGCACCCATTCAAGCTATTGTAAATAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGTTGTTCCGTGGTTGTCGCCCGCACCGCAGTCGAATCGCCGGCAGAAGGTCCGGCAGGAGaaattaa > 1:688100/1‑246 (MQ=255) aaTTGCAATTGGCGAAAAAGTGACTGTGCGCGGCTGGGTGCGCACCCGTCGCGACTCCAAAGCAGGCCTATCCTTTCTTGCCGTTTATGACGGTTCTTGCTTCGCACCCATTCAAGCTATTGTAAATAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGTTGTTCCGTGGTTGTCGCCGGCACCGTAGTCGAATCGCCGGCAGAAGGTCAGGCAGTAGAATTACAAGCGGAAAAAg < 1:299185/251‑1 (MQ=255) gtgCGCGGCTGGGTGCGCACCCGTCGCGACTCCAAAGCAGGCCTATCCTTTCTTGCCGTTTATGACGGTTCTTGCTTCGCACCCATTCAAGCTATTGTAAATAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGTTGTTCCGCGGTTGTCGCCGGCACCGCAGTTGCATCGCCGGCCGAAGGTCAGGCCGGAGAATTACAAACGGAAAAAAGGGAAGGGACCGGtttctttctctt > 1:532502/1‑241 (MQ=255) tgCGCGGCTGGGTGCGCACCCGTCGCGACTCCAAAGCAGGCCTATCCTTTCTTGCCGTTTATGACGGTTCTTGCTTCGCACCCATTCAAGCTATTGTAAATAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGTTGTTCCGTGGTTGTCGCCGGCACCGTAGTCGAATCGCCGGCAGAAGGTCAGGCAGTAGAATTACAAGCGGAAAAAGTGGAAGTCACCGGTTGGGTAGAAGAt < 1:597043/251‑1 (MQ=255) cccGTCGCGACTCCAAAGCAGGCCTATCCTTTCTTGCCGTTTATGACGGTTCTTGCTTCGCACCCATTCAAGCTATTGTAAATAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGTTGTTCCGTGGTTGTCGCCGGCACCGTAGTCGAATCGCCGGCAGAAGGTCAGGCAGTAGAATTACAAGCGGAAAAAGTGGAAGTCACCGGTTGGGTAGAAGATCCGGAAACTTACCCGATg < 1:619980/251‑1 (MQ=255) ccAAAGCAGGCCTATCCTTTCTTGCCGTTTATGACGGTTCTTGCTTCGCACCCATTCAAGCTATTGTAAATAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGTTGTTCCGTGGTTGTCGCCGGCACCGTAGTCGAATCGCCGGCAGAAGGTCAGGCAGTAGAATTACAAGCGGAAAAAGTGGAAGTCACCGGTTGGGTAGAAGATCCGGAAACTTACCCGATGGCGGCAAAACGt < 1:645869/251‑1 (MQ=255) aaGCAGGCCTATCCTTTCTTGCCGTTTATGACGGTTCTTGCTTCGCACCCATTCAAGCTATTGTAAATAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGTTGTTCCGTGGGTGTCGCCCGCACCGCAGGCGAATCCCCGGCAGAAGGGCAGGCAGGAGAATTACAAGCGGAAAAAATGGAAGACACCGGTTGGGTAGAAGAACCGGAAAATTAACCCATGGCGGCAAAACGTCa > 1:2085/1‑250 (MQ=255) ggCCTATCCTTTCTTGCCGTTTATGACGGTTCTTGCTTCGCACCCATTCAAGCTATTGTAAATAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGTTGTTCCGTGGTTGTGGCCGGCACCGTAGTCGAATCGCCGGCAGAAGGGCAGGGAGGAGAATTAAAAGCGGAAAAAGTGGGAGGCACCGGTTGGGGagaag > 1:640501/1‑211 (MQ=255) ggCCTATCCTTTCTTGCCGTTTATGACGGTTCTTGCTTCGCACCCATTCAAGCTATTGTAAATAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGTTGTTCCGTGGTTGTCGCCGGCACCGTAGTCGAATCGCCGGCAGAAGGTCAGGCAGTAGAATTACAAGCGGGAAAAGTGGAAGTCACCGGTTGGGGAGAAGATCCGGAAACTTACCCGATGGCGGCAAAACGTCACTCTAt > 1:308014/1‑251 (MQ=255) ggCCTATCCTTTCTTGCCGTTTATGACGGTTCTTGCTTCGCACCCATTCAAGCTATTGTAAATAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGTTGTTCCGTGGTTGTCGCCGGCACCGTAGTCGAATCGCCGGCAGAAGGTCAGGCAGTAGAATTACAAACGGAAAAAATGGAAGGCACCGGGTGGGTAGAAAATCCGGAAAATTAACCGATGGCGGGAAAAAGTCaacctat > 1:748629/1‑245 (MQ=255) ggCCTATCCTTTCTTGCCGTTTATGACGGTTCTTGCTTCGCACCCATTCAAGCTATTGTAAATAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGTTGTTCCGCGGGTGTCCCCCGCACCCCAGTCGAAACCCCCGCCGAAGGTCAGGCACGAGAATTTCAAGCGGAAAAAATGGGAGGCACCCGGTGGGGGGGAGAAACGGAAACTTAACCCAAGGCGGCAAAACGCCAccct > 1:304953/1‑246 (MQ=255) ccTTTCTTGCCGTTTATGACGGTTCTTGCTTCGCACCCATTCAAGCTATTGTAAATAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGTTGTTCCGTGGTTGTCGCCGGCACCGTAGTCGAATCGCCGGCAGAAGGTCAGGCAGTAGAATTACAAGCGGAAAAAGTGGAAGTCACCGGTTGGGTAGAAGATCCGGAAACTTACCCGATGGCGGCAAAACGTCACTCTATCGAatat < 1:160585/251‑1 (MQ=255) ttCTTGCCGTTTATGACGGTTCTTGCTTCGCACCCATTCAAGCTATTGTAAATAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGTTGTTCCGTGGTTGTCGCCGGCACCGTAGTCGAATCGCCGGCAGAAGGTCAGGCAGTAGAATTACAAGCGGAAAAAGTGGAAGTCACCGGTTGGGTAGAAGATCCGGAAACTTACCCGATGGCGGCAAAACGTCACTCTATCGaa < 1:620142/245‑1 (MQ=255) gTTTATGACGGTTCTTGCTTCGCACCCATTCAAGCTATTGTAAATAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGTTGTTCCGTGGTTGTCGCCGGCCCCGTAGTCGAATCGCCGGCAGAAGGTCAGGc < 1:518763/146‑1 (MQ=255) gTTTATGACGGTTCTTGCTTCGCACCCATTCAAGCTATTGTAAATAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGTTGTTCCGTGGTTGTCGCCGGCACCGTAGTCGAATCGCCGGCAGAAGGTCAGGCAGTAGAATTACAAGCGGAAAAAGTGGAAGTCACCGGTTGGGTAGAAGATCCGGAAACTTACCCGATGGCGGCAAAACGTCACTCTATCGAat < 1:702048/238‑1 (MQ=255) gTTCTTGCTTCGCACCCATTCAAGCTATTGTAAATAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGTTGTTCCGTGGTTGTCGCCGGCACCGTAGTCGAATCGCCGGCAGAAGGTCAGGc < 1:314915/136‑1 (MQ=255) cTTGCTTCGCACCCATTCAAGCTATTGTAAATAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGTTGTTCCGTGGTTGTCGCCGGCACCGTAGTCGAATCGCCGGCAGAAGGTCAGGCAGTAGAATTACAAGCGGAAAAAGTGGAAGTCACCGGTTGGGTAGAAGATCCGGAAACTTACCCGATGGCGGCAAAACGTCACTCTATCGAATATTTACGCGAAGTGGCGCACCTACGc < 1:713649/251‑1 (MQ=255) ttGCTTCGCACCCATTCAAGCTATTGTAAATAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGTTGTTCCGTGGTTGTCGCCGGCACCGTAGTCGAATCGCCGGCAGAAGGTCAGGCAGTAGAATTACAAGCGGAAAAAGTGGAAGTCACCGGTTGGGTAGAAGATCCGGAAACTTACCCGATGGCGGCAAAACGTCACTCTATCGAATATTTACGCGAAGTGGCGCACCTACGcc < 1:701714/251‑1 (MQ=255) ttGCTTCGCACCCATTCAAGCTATTGTAAATAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGTTGTTCCGTGGTTGTCGCCGGCACCGTAGTCGAATCGCCGGCAGAAGGTCAGGCAGTAGAATTACAAGCGGAAAAAGTGGAAGTCACCGGTTGGGTAGAAGATCCGGAAACTTACCCGATGGCGGCAAAACGTCACTCTATCGAATATTTACGCGAAGTGGCGCACCTACGcc < 1:593911/251‑1 (MQ=255) cTTCGCACCCATTCAAGCTATTGTAAATAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGTTGTTCCGTGGGTGTCGCCGGCACCGTAGTCGAATCGCCGGCAGAAGGGCAGGCAGGAGAATTACAAGCGGGAAAAGTGGAAGTCACCGGGTGGGGAGAAGATCCGGAAACTTACCCGATGGCGGCAAAACGTCACTCTATCGAatat > 1:77636/1‑223 (MQ=255) cACCCATTCAAGCTATTGTAAATAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGTTGTTCCGTGGTTGTCGCCGGCACCGTAGTCGAATCGCCGGCAGAAGGTCAGGCAGTAGAATTACAAGCGGAAAAAGTGGAAGTCACCGGTTGGGTAGAAGATCCGGAAACTTACCCGATGGCGGCAAAACGTCACTCTATCGAATATTTACGCGAAGTGGCGCACCTACGCCCGCGCAc < 1:419250/250‑1 (MQ=255) cccATTCAAGCTATTGTAAATAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGTTGTTCCGTGGTTGTCGCCGGCACCGTAGTCGAATCGCCGGCAGAAGGTCAGGCAGTAGAATTACAAGCGGAAAAAGTGGAAGTCACCGGTTGGGTAGAAGATCCGGAAACTTACCCGATGGCGGCAAAACGTCACTCTATCGAATATTTACGCGAAGTGGCGCACCTACGcc < 1:108494/241‑1 (MQ=255) cATTCAAGCTATTGTAAATAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGTTGTTCCGTGGGTGTCGCCGGCACCGTAGTCGAATCGCCGGCAGAAGGGCAGGCAGTAGAATTACAAGCGGAAAAAGAGGAAGTCACCGGTTGGGGAGAAGATCCGGAAACTTACCCGATGGCGGCAAAACGTCACTCTATCGAATATTTACGCGAAGGGGCGCACCTACGCCCGCGCACCAata > 1:343894/1‑251 (MQ=255) aTTCAAGCTATTGTAAATAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGTTGTTCCGTGGTTGTCGCCGGCACCGTAGTCGAATCGCCGGCAGAAGGTCAGGCAGTAGAATTACAAGCGGAAAAAGTGGAAGTCACCGGTTGGGTagaaga < 1:584750/167‑1 (MQ=255) aTTCAAGCTATTGTAAATAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGTTGTTCCGTGGTTGTCGCCGGCACCGTAGTCGAATCGCCGGCAGAAGGGCAGGCAGTAGAATTACAAGCGGAAAAAGTGGAAGTCACCGGGTGGGGAGAAGATCCGGAAACTTACCCGATGGCGGCAAAACGTCACTCTATCGAATATTTACGCGAAATGGCGCACCTACGCCCGCGCACCCatat > 1:570748/1‑251 (MQ=255) aTTCAAGCTATTGTAAATAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGGTGTTCCGTGGGTGTTGCCGGCACCGTAGTCGAATAGCCCGCAGAAGGGCAGGGAGGAGAATTACAAACGGGAAAAGAGGGAGTAACCGGGTGGGGAGAAGATCCGGGAAATTAACCGATGGCGGCAAAACGTCACTCTATCGGATATTTACGCGAAGAGGCGCCCCTCCGCCCCCGCAcccattt > 1:658580/1‑246 (MQ=255) tCAAGCTATTGTAAATAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGTTGTTCCGTGGTTGTCGCCGGCACCGTAGTCGAATCGCCGGCAGAAGGTCAGGCAGTAGAATTACAAGCGGAAAAAGTGGAAGTCACCGGTTGGGTAGAAGATCCGGAAACTTACCCGATGGCGGCAAAACGTCACTCTATCGAATATTTACGCGAAGTGGCGCACCTACGCCCGCGCACCAAtatta < 1:899273/251‑1 (MQ=255) aTTGTAAATAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGTTGTTCCGTGGTTTTCGCCGGCACCGTAGGCGAAACGCCGGCAGAAGGGCAGGCAGTAGAATTACAAACGGAAAAAAAGGAAGTCACCGGGTGGGGAGAAGATCCGGAAACATACCCGATGGCGGCCAAACGCCACTCTCTCGAATATTTACGCGAAGGGGCGCCCCCAACCCCCCGCACCCATATTATCggct > 1:357240/1‑248 (MQ=255) aTTGTAAATAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGTTGTTCCGTGGGTGTCGCCGGCACCCTAGTCGAATCGCCGGCAGAAGGGGCGGCAGGAGAATTACAAACGGGAAAAATGGAAACTACCGGTTGGGGAGAAGATCCCGAAAATTACCCGATGGCGGCAAAACGGCACTCTATCGAATATTTACGCGAAGTGGCGCACCTACGCCCGCGCACCAATATTATCGGTg > 1:60379/1‑250 (MQ=255) aaaTAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGTTGTTCCGTGGTTGTCGCCGGCACCGTAGTCGAATCGCCGGCAGAAGGTCAGGCAGTAGAATTACAAGCGGAAAAAGTGGAAGTCACCGGTTGGGTAGAAGATCCGGAAACTTACCCGATGGCGGCAAAACGTCACTCTATCGAATATTTACGCGAAGTGGCGCACCTACGCCCGCGCACCAATATTATCGGTGCAGTTg < 1:884315/251‑1 (MQ=255) tagtGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGGTGTTCCGTGGGTGTCGCCGGCACCGTAGTCGAATCGCCGGCAGAAGGGCAGGCAGTAGAATTACAAGCGGAAAAAGTGGAAGGCACCGGGTGGGGAGAAGAACCGGAAAATTACCCGATGGCGGCAAAAAGTCACTCTATCGAATATTTACGCGAAGGGGCGCACCTACGCCCGCGCCCCAATATTATCGGTGGAGTTGcc > 1:344298/4‑250 (MQ=255) taatGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGTTGTTCCGTGGTTGTCGCCGGCACCGTAGTCGAATCGCCGGCAGAAGGTCAGGCAGTAGAATTACAAGCGGAAAAAGTGGAAGTCACCGGTTGGGTAGAAGATCCGGAAACTTACCCGATGGCGGCAAAACGTCACTCTATCGAATATTTACGCGAAGTGGCGCACCTACGCCCGCGCACCAATATTATCGGTGCAGTTGccc < 1:265040/251‑1 (MQ=255) taatGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGTTGTTCCGTGGTTGTCGCCGGCACCGTAGTCGAATCGCCGGCAGAAGGGCAGGCAGGAGAATTACAAGCGGAAAAAATGGAAGTCACCGGGTGGGTAGAAGATCCGGAAAATTACCCGATGGCGGCAAAAAGGCACTCTATCGAATATTTACGCGAAGGGGCGCACCTACGCCCGCCCACCAATATTATCGGTGCAGTTGcc > 1:864634/1‑250 (MQ=255) gACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGTTGTTTCGTGGTTTTCGCCGGCACCGTAGGCGAATCGCCGGCAGAAGGTCAGGCAGTAGAATTACAAGCGGGAAAAATGGAAGTCACCGGTTGGGGAGAAGATCCGGAAACTTACCCGATGGCGGCAAAACGTCACTCTATCGAATATTTACGCGAAGAGGCGCACCTACCCCCGCGCACCCAtatta > 1:351446/1‑232 (MQ=255) gCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGGTGTTCCGTGGTTGTCGCCGGCACCGTAGGCGAAACGCCGGCAGAAGGGCAGGCAGTAGAATTACAAACGGGAAAAGTGGAAGTCACCGGTTGGGGAGAAGATTCCGAAACTTACCCGATGGCGGGAAAACGTCACTCTATCGAATATTTATGCGAAGTGGCGCACCCACGCCCGCCCACCCATATTATCGGTGCAGTTGCCCGTGTTCGTc > 1:88768/1‑250 (MQ=255) aTCTGAAGTTTTACGCTTAACGGCGGGTTGTTCCGTGGTTGTCGCCGGCACCGTAGTCGAATCGCCGGCAGAAGGTCAGGCAGTAGAATTACAAGCGGAAAAAGTGGAAGTCACCGGTTGGGTAGAAGATCCGGAAACTTACCCGATGGCGGCAAAACGTCACTCTATCGAATATTTACGCGAAGTGGCGCACCTACGCCCGCGCACCAATATTATCGGTGCAGTTGCCCGTGTTCGTCATTGCTTGGcac < 1:231746/251‑1 (MQ=255) aTCTGAAGTTTTACGCTTAACGGCGGGTTGTTCCGTGGTTGTCGCCGGCACCGTAGTCGAATCGCCGGCAGAAGGTCAGGCAGTAGAATTACAAGCGGAAAAAGTGGAAGTCACCGGTTGGGTAGAAGATCCGGAAACTTACCCGATGGCGGCAAAACGTCACTCTATCGAATATTTACGCGAAGTGGCGCACCTACGCCCGCGCACCAATATTATCGGTGCAGTTGCCCGTGTTCGTCATTGCTTGGcac < 1:809615/251‑1 (MQ=255) aaGTTTTACGCTTAACGGCGGGTTGTTCCGTGGTTGTCGCCGGCACCGTAGTCGAATCGCCGGCAGAAGGTCAGGCAGTAGAATTACAAGCGGAAAAAGTGGAAGTCACCGGTTGGGTAGAAGATCCGGAAACTTACCCGATGGCGGCAAAACGTCACTCTATCGAATATTTACGCGAAGTGGCGCACCTACGCCCGCGCACCAATATTATCGGTGCAGTTGCCCGTGTTCGTCATTGCTTGGCACAAGcc < 1:612320/251‑1 (MQ=255) gTTTTACGCTTAACGGCGGGTTGTTTCGTGGGTGTCGCCGGCACCGTAGTCGAATCGCCGGCAGAAGGGCAGGCAGGAGAATTACAAGCGGAAAAAGTGGAAGTCACCGGTTGGGGAGAAGATCCGGAAAATTACCCGGTGGCGGCAAAAAGGCACCCTATCGAATATTTACGCGAAGTGGCGCACCCACGcc > 1:593643/1‑193 (MQ=255) aCGCTTAACGGCGGGTTGTTCCGTGGTTGTCGCCGGCACCGTAGTCGAATCGCCGGCAGAAGGTCAGGCAGTAGAATTACAAGCGGAAAAAGTGGAAGTCACCGGTTGGGTAGAAGATCCGGAAACTTACCCGATGGCGGCAAAACGTCACTCTATCGAATATTTACGCGAAGTGGCGCACCTACGCCCGCGCACCAATATTATCGGTGCAGTTGCCCGTGTTCGTCATTGCTTGGCACAAGCCATTCATc < 1:289307/251‑1 (MQ=255) ttgttCCGTGGTTGTCGCCGGCACCGTAGTCGAATCGCCGGCAGAAGGTCAGGCAGTAGAATTACAAGCGGAAAAAGTGGAAGTCACCGGTTGGGTAGAAGATCCGGAAACTTACCCGATGGCGGCAAAACGTCACTCTATCGAATATTTACGCGAAGTGGCGCACCTACGCCCGCGCACCAATATTATCGGTGCAGTTGCCCGTGTTCGTCATTGCTTGGCACAAGCCATTCATCGTTTCTTTCATGAAc < 1:616068/251‑1 (MQ=255) gttCCGTGGTTGTCGCCGGCACCGTAGTCGAATCGCCGGCAGAAGGTCAGGCAGTAGAATTACAAGCGGAAAAAGTGGAAGTCACCGGTTGGGTAGAAGATCCGGAAACTTACCCGATGGCGGCAAAACGTCACTCTATCGAATATTTACGCGAAGTGGCGCACCTACGCCCGCGCACCAatat > 1:381675/1‑184 (MQ=255) gttCCGTGGTTGTCGCCGGCACCGTAGTCGAATCGCCGGCAGAAGGTCAGGCAGTAGAATTACAAGCGGAAAAAGTGGAAGTCACCGGTTGGGTAGAAGATCCGGAAACTTACCCGATGGCGGCAAAACGTCACTCTATCGAATATTTACGCGAAGTGGCGCACCTACGCCCGCGCACCAATATTATCGGTGCAGTTGCCCGTGTTCGTCATTGCTTGGCACAAGCCATTCATCGTTTCTTTCATGAACaa > 1:172298/1‑251 (MQ=255) ccGTGGTTGTCGCCGGCACCGTAGTCGAATCGCCGGCAGAAGGTCAGGCAGTAGAATTACAAGCGGAAAAAGTGGAAGTCACCGGTTGGGTAGAAGATCCGGAAACTTACCCGATGGCGGCAAAACGTCACTCTATCGAATATTTACGCGAAGTGGCGCACCTACGCCCGCGCACCAATATTATCGGTGCAGTTGCCCGTGTTCGTCATTGCTTGGCACAAGCCATTCATCGTTTCTTTCATGAACAAGGt < 1:209940/251‑1 (MQ=255) | GGTATATATGACAAAAATTGCATCAATTGTAAATGTGCTGCAAGGCAAAATTGCAATTGGCGAAAAAGTGACTGTGCGCGGCTGGGTGCGCACCCGTCGCGACTCCAAAGCAGGCCTATCCTTTCTTGCCGTTTATGACGGTTCTTGCTTCGCACCCATTCAAGCTATTGTAAATAATGACATTGCCAATTATGAATCTGAAGTTTTACGCTTAACGGCGGGTTGTTCCGTGGTTGTCACCGGCACCGTAGTCGAATCGCCGGCAGAAGGTCAGGCAGTAGAATTACAAGCGGAAAAAGTGGAAGTCACCGGTTGGGTAGAAGATCCGGAAACTTACCCGATGGCGGCAAAACGTCACTCTATCGAATATTTACGCGAAGTGGCGCACCTACGCCCGCGCACCAATATTATCGGTGCAGTTGCCCGTGTTCGTCATTGCTTGGCACAAGCCATTCATCGTTTCTTTCATGAACAAGGT > 1/1154010‑1154487 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |