Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,151,535	T→C	V471A (GTG→GCG)	unknown →	Autoinducer 2 (AI‑2) kinase LsrK (EC 2.7.1.‑)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,151,535	0	T	C	100.0%	167.6 / NA	63	V471A (GTG→GCG)	unknown	Autoinducer 2 (AI‑2) kinase LsrK (EC 2.7.1.‑)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (36/27);  total (36/27)

CCTTGGAAGAAAATGCTGCTATCGTTTCTTCCTGCAATTTGGATCAGGTGCAAAAATTCTCAGGAGTTAAATTAACCTCCATTGTCTTTGCCGGCGGCGGGTCTAAAGGCAAATTATGGAGCCAGATCTTAGCCGATGTCACCGGATTAACGGTAAACATTCCGATTGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATTCGACCTTAGCCGAGGTGGGGGAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATACCAAGCTCACAAAGCACAATGGGCAGATACTTATAAAAAACAATTACAACTGGTTGATAGCGGATTAACTACTTCTTTGTGGAAGGCACCAGGGTTGTAGCGAACGATAAAAAAATCGTAGGGGGGACAACTCTCTACGATTTTTTGTTGATATACCGTAAAAAACATCGGTAAAACCC  >  1/1151292‑1151777                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   ccTTGGAAGAAAATGCTGCTATCGTTTCTTCCTGCAATTTGGATCAGGTGCAAAAATTCTCAGGAGTTAAATTAACCTCCATTGTCTTTGCCGGCGGCGGGTCTAAAGGCAAATTATGGAGCCAGATCTTAGCCGATGTCACCGGATTAACGGTAAACATTCCGATTGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCggggaaa                                                                                                                                                                                                                                             >  1:414100/1‑248 (MQ=255)      gaagaaAATGCTGCTATCGTTTCTTCCTGCAATTTGGATCAGGTGCAAAAATTCTCAGGAGTTAAATTAACCTCCATTGTCTTTGCCGGCGGCGGGTCTAAAGGCAAATTATGGAGCCAGATCTTAGCCGATGTCACCGGATTAACGGTAAACATTCCGATTGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAAc                                                                                                                                                                                                                                         <  1:751800/250‑1 (MQ=255)                          ttcttcCTGCAATTTGGATCAGGTGCAAAAATTCTCAGGAGTTAAATTAACCTCCATTGTCTTTGCCGGCGGCGGGTCTAAAGGCAAATTATGGAGCCAGATCTTAGCCGATGTCACCGGATTAACGGTAAACATTCCGATTGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAAtt                                                                                                                                                                                                                               <  1:482650/240‑1 (MQ=255)                                     aTTTGGATCAGGTGCAAAAATTCTCAGGAGTTAAATTAACCTCCATTGTCTTTGCCGGCGGCGGGTCTAAAGGCAAATTATGGAGCCAGATCTTAGCCGATGTCACCGGATTAACGGTAAACATTCCGATTGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAAt                                                                                                                                                                                                         <  1:363283/251‑1 (MQ=255)                                     aTTTGGATCAGGTGCAAAAATTCTCAGGAGTTAAATTAACCTCCATTGTCTTTGCCGGCGGCGGGTCTAAAGGCAAATTATGGAGCCAGATCTTAGCCGATGTCACCGGATTAACGGTAAACATTCCGATTGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAAATGGGTAAATTTGAACGGCAACATCAACCGAAt                                                                                                                                                                                                         >  1:598396/1‑251 (MQ=255)                                                gTGCAAAAATTCTCAGGAGTTAAATTAACCTCCATTGTCTTTGCCGGCGGCGGGTCTAAAGGCAAATTATGGAGCCAGATCTTAGCCGATGTCACCGGATTAACGGTAAACATTCCGATTGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGGAAAAACTGGTTAAATTTTAACGGCAACATCAACCGAATCCCGATAACTa                                                                                                                                                                                              >  1:872627/1‑251 (MQ=255)                                                gTGCAAAAATTCTCAGGAGTTAAATTAACCTCCATTGTCTTTGCCGGCGGCGGGTCTAAAGGCAAATTATGGAGCCAGATCTTAGCCGATGTCACCGGATTAACGGTAAACATTCCGATTGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTa                                                                                                                                                                                              >  1:439114/1‑251 (MQ=255)                                                   cAAAAATTCTCAGGAGTTAAATTAACCTCCATTGTCTTTGCCGGCGGCGGGTCTAAAGGCAAATTATGGAGCCAGATCTTAGCCGATGTCACCGGATTAACGGTAAACATTCCGATTGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGc                                                                                                                                                                                           <  1:870574/251‑1 (MQ=255)                                                        aTTCTCAGGAGTTAAATTAACCTCCATTGTCTTTGCCGGCGGCGGGTCTAAAGGCAAATTATGGAGCCAGATCTTAGCCGATGTCACCGGATTAACGGTAAACATTCCGATTGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTtat                                                                                                                                                                                      >  1:584872/1‑251 (MQ=255)                                                          tctcAGGAGTTAAATTAACCTCCATTGTCTTTGCCGGCGGCGGGTCTAAAGGCAAATTATGGAGCCAGATCTTAGCCGATGGCACCGGATTAACGGTAAACATTCCGATTGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATAc                                                                                                                                                                                    <  1:442103/251‑1 (MQ=255)                                                                     aaaTTAACCTCCATTGTCTTTGCCGGCGGCGGGTCTAAAGGCAAATTATGGAGCCAGATCTTAGCCGATGTCACCGGATTAACGGTAAACATTCCGATTGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATACCAAGCTCACaa                                                                                                                                                                         <  1:859423/251‑1 (MQ=255)                                                                       aTTAACCTCCATTGTCTTTGCCGGCGGCGGGTCTAAAGGCAAATTATGGAGCCAGATCTTAGCCGATGTCACCGGATTAACGGTAAACATTCCGATTGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATACCAAGCTCACAAAg                                                                                                                                                                       >  1:774717/1‑251 (MQ=255)                                                                          aaCCTCCATTGTCTTTGCCGGCGGCGGGTCTAAAGGCAAATTATGGAGCCAGATCTTAGCCGATGTCACCGGATTAACGGTAAACATTCCGATTGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATACCAAGCTCACAAAGcac                                                                                                                                                                    <  1:348556/251‑1 (MQ=255)                                                                               ccATTGTCTTTGCCGGCGGCGGGTCTAAAGGCAAATTATGGAGCCAGATCTTAGCCGATGTCACCGGATTAACGGTAAACATTCCGATTGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATACCAAGCTCACAAAGcac                                                                                                                                                                    <  1:423088/246‑1 (MQ=255)                                                                               ccATTGTCTTTGCCGGCGGCGGGTCTAAAGGCAAATTATGGAGCCAGATCTTAGCCGATGTCACCGGATTAACGGTAAACATTCCGATTGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATACCAAGCTCACAAAGCACAATgg                                                                                                                                                               <  1:195019/251‑1 (MQ=255)                                                                                 aTTGTCTTTGCCGGCGGCGGGTCTAAAGGCAAATTATGGAGCCAGATCTTAGCCGATGTCACCGGATTAACGGTAAACATTCCGATTGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATACCAAGCTCACAAAGCACAATGGGc                                                                                                                                                             <  1:824343/251‑1 (MQ=255)                                                                                    gTCTTTGCCGGCGGCGGGTCTAAAGGCAAATTATGGAGCCAGATCTTAGCCGATGTCACCGGATTAACGGTAAACATTCCGATTGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAAtt                                                                                                                                                                                                                               <  1:158142/182‑1 (MQ=255)                                                                                    gTCTTTGCCGGCGGCGGGTCTAAAGGCAAATTATGGAGCCAGATCTTAGCCGATGTCACCGGATTAACGGTAAACATTCCGATTGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAAcc                                                                                                                                                                                                             <  1:407676/200‑1 (MQ=255)                                                                                      cTTTGCCGGCGGCGGGTCTAAAGGCAAATTATGGAGCCAGATCTTAGCCGATGTCACCGGATTAACGGTAAACATTCCGATTGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATACCAAGCTCACAAAGCACAATGGGCAGATa                                                                                                                                                        <  1:864446/251‑1 (MQ=255)                                                                                                     gtttAAAGGCAAATTATGGAGCCAGATCTTAGCCGATGTCACCGGATTAACGGTAAACATTCCGATTGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGGAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATACCAAGCTCACAAAGCACAATGGGCAGATATTTATAAAAAAAAAt                                                                                                                                         >  1:871066/4‑251 (MQ=255)                                                                                                     gTCTAAAGGCAAATTATGGAGCCAGATCTTAGCCGATGTCACCGGATTAACGGTAAACATTCCGATTGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATACCAAGCTCACAAAGCTCAATGGGCAGATATTTATAAAAAACAAt                                                                                                                                         >  1:216211/1‑251 (MQ=255)                                                                                                      tCTAAAGGCAAATTATGGAGCCAGATCTTAGCCGATGTCACCGGATTAACGGTAAACATTCCGATTGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGGAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATACCa                                                                                                                                                                                  >  1:400175/1‑209 (MQ=255)                                                                                                            ggCAAATTATGGAGCCAGATCTTAGCCGATGTCACCGGATTAACGGTAAACATTCCGATTGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATAc                                                                                                                                                                                    <  1:728920/201‑1 (MQ=255)                                                                                                             gCAAATTATGGAGCCAGATCTTAGCCGATGTCACCGGATTAACGGTAAACATTCCGATTGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATACCAAGCTCACAAAGCACAATGGGCAGATATTTATAAAAAACAATTACAAc                                                                                                                                   <  1:245507/249‑1 (MQ=255)                                                                                                              cAAATTATGGAGCCAGATCTTAGCCGATGTCACCGGATTAACGGTAAACATTCCGATTGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGGAAAAACTGGGTAAATTTGAACGGCAACATCAAACGAATTCCGATAACTAAGGTTTTTACCAAGCTCAAAAAGCAAAATGGGCAGAAATTTATAAAAAACAATTACAACTgg                                                                                                                                >  1:78209/1‑251 (MQ=255)                                                                                                                       gAGCCAGATCTTAGCCGATGTCACCGGATTAACGGTAAACATTCCGATTGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGGAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATACCAAGCTCACAAAGCACAATGGGCAGATATTTATAAAAAAAAATTACAACTGGTTGATAGCg                                                                                                                       >  1:201116/1‑251 (MQ=255)                                                                                                                        aGCCAGATCTTAGCCGATGTCACCGGATTAACGGTAAACATTCCGATTGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATACCAAGCTCACAAAGCACAATgg                                                                                                                                                               <  1:198405/210‑1 (MQ=255)                                                                                                                             gATCTTAGCCGATGTCACCGGATTAACGGTAAACATTCCGATTGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATGACTATGCTTTATACCAAGCTCACAAAGCACAATGGGCAGATATTTATAAAAAACAATTACAACTg                                                                                                                                 >  1:429467/1‑235 (MQ=255)                                                                                                                             gATCTTAGCCGATGTCACCGGATTAACGGTAAACATTCCGATTGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATACCAAGCTCACAAAGCACAATGGGCAGATATTTATAAAAAACAATTACAACTGGTTGATAGCGGATTaa                                                                                                                 >  1:775665/1‑251 (MQ=255)                                                                                                                              aTCTTAGCCGATGTCACCGGATTAACGGTAAACATTCCGATTGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGGAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAAc                                                                                                                                                                                                >  1:631501/1‑171 (MQ=255)                                                                                                                              aTCTTAGCCGATGTCACCGGATTAACGGTAAACATTCCGATTGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAATTAGTTAAATTTGAACGGCAACATGAACCCAATCCCGATAACAATGCTTTATACCCAGCTCACAAAGCACAAAGGGCAGATATTTATAAAAAACAATTAAAACTGGATGAGAGGGGATTaa                                                                                                                 >  1:784371/1‑250 (MQ=255)                                                                                                                                       gATGTCACCGGATTAACGGTAAACATTCCGATTGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATACCAAGCTCACAAAGCACAATGGGCAGATATTTATAAAAAACAATTACAACTGGGTGATAGCGGATTAACTACTTCttt                                                                                                       >  1:99631/1‑251 (MQ=255)                                                                                                                                                 gATTAACGGTAAACATTCCGATTGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAGTTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATACCAAGCTCACAAAGCACAATGGGCAGATATTTATAAAAAACAATTACAACTGGTTGATAGCGGATTAACTACTTCTTTGTAGAAGGc                                                                                              <  1:357319/250‑1 (MQ=255)                                                                                                                                                 gATTAACGGTAAACATTCCGATTGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTTAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATTCCCAGCTCACAAAGCACAATGGGCAGATATTTATAAAAAACAATTACAACTGGTTGATAGCGGATTAACTACTTCTTTGTAGAAGGCa                                                                                             >  1:23436/1‑251 (MQ=255)                                                                                                                                                 gATTAACGGTAAACATTCCGATTGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATAc                                                                                                                                                                                    >  1:90446/1‑164 (MQ=255)                                                                                                                                                      aCGGTAAACATTCCGATTGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATACCAAGCTCACAAAGcac                                                                                                                                                                    <  1:346797/175‑1 (MQ=255)                                                                                                                                                      aCGGTAAACATTCCGATTGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATACCAAGCTCACAAAGCACAATGGGCAGATATTtat                                                                                                                                                   >  1:677111/1‑192 (MQ=255)                                                                                                                                                      aCGGTAAACATTCCGATTGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAAATGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATACCAAGCTCACAAAGCACAAAGGGCAGATATTTATAAAAAACAATTACAACTGGTTGATAGCGGATTAACTACTTCTTTGTAGAAGGCACCAgg                                                                                        >  1:599234/1‑251 (MQ=255)                                                                                                                                                             aCATTCCGATTGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATACCAAGCTCACAAAGCACAATGGGCAGATATTTATAAAAAACAATTACAACTGGTTGATAGCGGATTAACTACTTCTTTGTAGAAGGCACCAGGGTTGTAg                                                                                 <  1:712643/251‑1 (MQ=255)                                                                                                                                                              cATTCCGATTGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATAc                                                                                                                                                                                    >  1:91793/1‑151 (MQ=255)                                                                                                                                                                       tGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATACCAAGCTCACAAAGCACAATGGGCAGATATTTATAAAAAACAATTACAACTGGTTGATAGCGGATTAACTACTTCTTTGTAGAAGGCACCAGGGTTGTAGCGAATGATaa                                                                       >  1:678313/1‑251 (MQ=255)                                                                                                                                                                                       gcgcTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCCACATCAACCGAATCCCGATAACTATGc                                                                                                                                                                                           >  1:823838/1‑119 (MQ=255)                                                                                                                                                                                       gcgcTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAAc                                                                                                                                                                                                              <  1:652220/100‑1 (MQ=255)                                                                                                                                                                                       gcgcTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGc                                                                                                                                                                                           >  1:820824/1‑119 (MQ=255)                                                                                                                                                                                       gcgcTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATACCAAGCTCACAAAGCACAATGGGCa                                                                                                                                                            <  1:135464/150‑1 (MQ=255)                                                                                                                                                                                       gcgcTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATACCAAGCTCACAAAGCACAATGGGCAGATATTTATAAAAAACAATTACAACTGGTTGATAGCGGATTAACTACTTCTTtgtag                                                                                                   >  1:125175/1‑205 (MQ=255)                                                                                                                                                                                       gcgcTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATACCAAGCTCACAAAGCACAATGGGCAGATATTTATAAAAAACAATTACAACTGGTTGATAGCGGATTAACTACTTCTTTGTAGAAGGCACCAGGGTTGTAGCGAATGATAAAAAAATCGTAgggggg                                                       >  1:831482/1‑251 (MQ=255)                                                                                                                                                                                            gggTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATACCAAGCTCACAAAGCACAATGGGCAGATATTtat                                                                                                                                                   >  1:280073/1‑154 (MQ=255)                                                                                                                                                                                                        tGCCGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATACCAAGCTCACAAAGCACAATGGGCAGATATTTATAAAAAACAATTACAACTGGTTGATAg                                                                                                                         <  1:250204/168‑1 (MQ=255)                                                                                                                                                                                                          ccGCAGGCGTCGGAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAAACCCGATAACTATGCTTTATACCAAGCTCACAAAGCACAATGGGCAGATATTTATAAAAAACAATTACAACAGGTTGATAGCGGATTAACTACTTCTTTGTAGAAAGCACCAGGGGCGTAGCGAATGATAAAAAAATCGTAGGGGGGACAACTCTCTACGAttttt                                    >  1:336902/1‑251 (MQ=255)                                                                                                                                                                                                                      gAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATACCAAGCTCACAAAGCACAATGGGCAGATATTTATAAAAAACAATTACAACTGGTTGATAGCGGATTAACTACTTCTTTGTAGAAGGCACCAGGGTTGTAGTGAATGATAAAAAAATCGTAGGGGGGACAACTCTCTACGATTTTTTGTTGATATAc                         <  1:253458/250‑1 (MQ=255)                                                                                                                                                                                                                      gAGTCGGCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATACCAAGCTCACAAAGCACAATGGGCAGATATTTATAAAAAACAATTACAACTGGTTGATAGCGGATTAACTACTTCTTTGTAGAAGGCACCAGGGTTGTAGCGAATGATAAAAAAATCGTAGGGGGGACAACTCTCTACGATTTTTTGTTGATATAc                         <  1:292046/250‑1 (MQ=255)                                                                                                                                                                                                                            gCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATACCAAGCTCACAAAGCACAATGGGCAGATATTTATAAAAAACAATTACAACTGGTTGATAGCGGATTAACTACTTCTTTGTAGAAGGCACCAGGGTTGTAGCGAATGATAAAAAAATCGTAGGGGGGACAACTCTCTACGATTTTTTGTTGATATACCGTaaaa                  >  1:194914/1‑251 (MQ=255)                                                                                                                                                                                                                            gCATTTATCCGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATACCAAACTCACAAAGCACAAAGTGCAGATATTTATAAAAAACAATTACAACTGGTTGATAGCGGATTAACTACTTCTTTGTAGAAGGCACCAGGGTTGTAGCGAATGATAAAAAAATCGTATGGGGGACAACTCTCTACGATTTTTTGTTGATATACCGTaaaa                  >  1:551592/1‑251 (MQ=255)                                                                                                                                                                                                                                 tatccGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATACCAAGCTCACAAAGCACAATGGGCAGATATTTATAAAAAACAATTACAACTGGTTGATAGCGGATTAacta                                                                                                              >  1:125405/5‑154 (MQ=255)                                                                                                                                                                                                                                 tatccGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATACCAAGCTCACAAAGCACAATGGGCAGATATTTATAAAAAACAATTACAACTGGTTGATAGCGGATTAACTACTTCTTTGTAGAAGGCACCAGGGTTGTAGCGAATGATAAAAAAATCGTAGGGGGGACAACTCTCTACGATTTTTTGTTGATATACCGTAAAAAACAt             >  1:33208/5‑251 (MQ=255)                                                                                                                                                                                                                                  atccGACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATACCAAGCTCACAAAGCACAATGGGCAGATATTTATAAAAAACAATTACAACTGGTTGATAGCGGATTAACTACTTCTTTGTAGAAGGCACCAGGGTTGTAGCGAATGATAAAAAAATCGTAGGGGGGACAACTCTCTACGATTTTTTGTTGATATACCGTAAAAAACAt             >  1:113542/4‑250 (MQ=255)                                                                                                                                                                                                                                      gACCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATACCAAGCTCACAAAGCACAATGGGCAGATATTTATAAAAAACAATTACAACTGGTTGATAGCGGATTAACTACTTCTTtgtag                                                                                                   >  1:410807/1‑158 (MQ=255)                                                                                                                                                                                                                                       aCCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATACCAAGc                                                                                                                                                                               >  1:609791/1‑83 (MQ=255)                                                                                                                                                                                                                                       aCCTTAGCCGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATACCAAGCTCACAAAGCACAATGGGCAGATATTTATAAAAAACAATTACAACTGGTTGATAGCGGATTAACTACTTCTTTGTAGAAGGCACCAGGGTTGTAGCGAATGATAAAAAAATCGTAGGGGGGACAACTCTCTACGATTTTTTGTTGATATACCGTAAAAAACATCGGTaa       >  1:538333/1‑251 (MQ=255)                                                                                                                                                                                                                                              ccGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATACCAAGCTCACAAAGCACAATGGGCAGATATTTATAAAAAACAATTACAACTGGTTGATAGCGGATTAACTACTTCTTTGTAGAAGGCACCAGGGTTGTAGCGAATGATAAAAAAATCGTAGGGGGGACAACTCTCTACGATTTTTTGTTGATATACCGTAAAAAACATCGGTAAAAccc  <  1:822620/249‑1 (MQ=255)                                                                                                                                                                                                                                              ccGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATACCAAGCTCACAAAGCACAATGGGCAGATATTTATAAAAAACAATTACAACTGGTTGATAGCGGATTAACTACTTCTTTGTAGAAGGCACCAGGGTTGTAGCGAATGATAAAAAAATCGTAGGGGGGACAACTCTCTACGATTTTTTGTTGATATACCGTAAAAAACATCGGTAAAAccc  <  1:303988/249‑1 (MQ=255)                                                                                                                                                                                                                                              ccGAGGCGGGGAAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATACCAAGCTCACAAAGCACAATGGGCAGATATTTATAAAAAACAATTACAACTGGTTGATAGCGGATTAACTACTTCTTTGTAGAAGGCACCAGGGTTGTAGCGAATGATAAAAAAATCGTAGGGGGGACAACTCTCTACGATTTTTTGTTGATATACCGTAAAAAACATCGGTAAAAccc  <  1:548765/249‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                   CCTTGGAAGAAAATGCTGCTATCGTTTCTTCCTGCAATTTGGATCAGGTGCAAAAATTCTCAGGAGTTAAATTAACCTCCATTGTCTTTGCCGGCGGCGGGTCTAAAGGCAAATTATGGAGCCAGATCTTAGCCGATGTCACCGGATTAACGGTAAACATTCCGATTGTCAAAGAAGCTACAGCGCTGGGTTGTGCCATTGCCGCAGGCGTCGGAGTCGGCATTTATTCGACCTTAGCCGAGGTGGGGGAAAAACTGGTTAAATTTGAACGGCAACATCAACCGAATCCCGATAACTATGCTTTATACCAAGCTCACAAAGCACAATGGGCAGATACTTATAAAAAACAATTACAACTGGTTGATAGCGGATTAACTACTTCTTTGTGGAAGGCACCAGGGTTGTAGCGAACGATAAAAAAATCGTAGGGGGGACAACTCTCTACGATTTTTTGTTGATATACCGTAAAAAACATCGGTAAAACCC  >  1/1151292‑1151777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑