Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,145,747	T→C	I183M (ATA→ATG)	unknown ←	Autoinducer 2 (AI‑2) ABC transporter, permease protein LsrD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,145,747	0	T	C	100.0%	196.9 / NA	69	I183M (ATA→ATG)	unknown	Autoinducer 2 (AI‑2) ABC transporter, permease protein LsrD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (37/32);  total (37/32)

GCCCAACACCACGGCGGTCAATACCGGCATCAGCAGGGAACTGCCTAAGTCGGAGCGGGCGGAACCGAAATAAGACACCAGCAACACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGTATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACGCACCACCGCCGAATAGGTACATAGTGCCTAAGGTGATCACCAATGGATTCACTTTGGTGTAGAGAATCAATCCGGCATTAATC  >  1/1145525‑1145984                                                                                                                                                                                                                               |                                                                                                                                                                                                                                               gCCCAACACCACGGCGGTCAATACCGGCATCAGCAGGGAACTGCCTAAGTCGGAGCGGGCGGAACCGAAATAAGACACCAGCAACACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATCTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGAGCTTCTGCCGATGGTGGTTTTGTGCACTAATAACCAAAAGAACAGCGTCATTa                                                                                                                                                                                                                    >  1:733987/1‑251 (MQ=255)      acacCACGGCGGTCAATACCGGCATCAGCAGGGAACTGCCTAAGTCGGAGCGGGCGGAACCGAAATAAGACACCAGCAACACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGa                                                                                                                                                                                                               >  1:127886/1‑251 (MQ=255)                ggTCAATACCGGCATCAGCAGGGAACTGCCTAAGTCGGAGCGGGCGGAACCGAAATAAGACACCAGCAACACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGa                                                                                                                                                                                                     >  1:842483/1‑251 (MQ=255)                               cagcagGGAACTGCCTAAGTCGGAGCGGGCGGAACCGAAATAAGACACCAGCAACACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATg                                                                                                                                                                                       >  1:390921/1‑250 (MQ=255)                                 gcagGGACCTGCCTAAGTCGGAGCGGGCGAAACCAAAAAAAGCCCCCAGCACCACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATtaa                                                                                                                                                                                                                                           <  1:458734/196‑1 (MQ=255)                                 gcagGGAACTGCCTAAGTCGGAGCGGGCGGAACCGAAATAAGACACCAGCAACACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCg                                                                                                                                                                                    >  1:478479/1‑251 (MQ=255)                                    gggAACTGCCTAAGTCGGAGCGGGCGGAACCGAAATAAGACACCAGCAACACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAAt                                                                                                                                                                                 >  1:325123/1‑251 (MQ=255)                                           gCCTAAGTCGGAGCGGGCGGAACCGAAATAAGACACCAGCAACACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGttt                                                                                                                                                                          >  1:152508/1‑251 (MQ=255)                                                     ggggggcgaaaactaaaaaaacaccccAGCAACACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGa                                                                                                                                                                 <  1:183787/225‑1 (MQ=255)                                                              gAACCGAAATAAGACACCAGCAACACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCaa                                                                                                                                                       >  1:241243/1‑251 (MQ=255)                                                              aaacaaaaaaaagccacaaGCAACACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCaa                                                                                                                                                       <  1:586771/233‑1 (MQ=255)                                                                    aaaaaaaacaccacaaaCACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATc                                                                                                                                                <  1:369293/236‑1 (MQ=255)                                                                                               aaCGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAAcc                                                                                                                     <  1:41819/251‑1 (MQ=255)                                                                                                 cGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATtaa                                                                                                                                                                                                                                           <  1:505807/132‑1 (MQ=255)                                                                                                 cGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAAc                                                                                                                      >  1:854341/1‑248 (MQ=255)                                                                                                 cGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAAc                                                                                                                      >  1:897291/1‑248 (MQ=255)                                                                                                            cGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCa                                                                                                        >  1:730188/1‑251 (MQ=255)                                                                                                              ccAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCaaa                                                                                                      >  1:598171/1‑251 (MQ=255)                                                                                                               cAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCaaaa                                                                                                     >  1:606894/1‑251 (MQ=255)                                                                                                                     aGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGa                                                                                                >  1:467420/1‑250 (MQ=255)                                                                                                                       gAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCaa                                                                                                                                                       >  1:205112/1‑194 (MQ=255)                                                                                                                             aTCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAg                                                                                                                                         >  1:225921/1‑201 (MQ=255)                                                                                                                               cAATAATAATGTGTGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACg                                                                                     >  1:264992/1‑251 (MQ=255)                                                                                                                               cAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCAt                                                                                                                         >  1:276386/1‑215 (MQ=255)                                                                                                                               cAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAAcc                                                                                                                     >  1:279001/1‑219 (MQ=255)                                                                                                                               cAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACg                                                                                     >  1:283550/1‑251 (MQ=255)                                                                                                                                aataataatGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACGc                                                                                    <  1:515863/251‑1 (MQ=255)                                                                                                                                 ataataatGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATaa                                                                                                                                                                               <  1:540100/160‑1 (MQ=255)                                                                                                                                 ataataatGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGAt                                                                                                                                 >  1:829491/1‑205 (MQ=255)                                                                                                                                 ataataatGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACGca                                                                                   >  1:505138/1‑251 (MQ=255)                                                                                                                                   aataatGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACGcacc                                                                                 <  1:483812/251‑1 (MQ=255)                                                                                                                                              acgaATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACGCACCACCGCCGAAt                                                                       <  1:75567/250‑1 (MQ=255)                                                                                                                                                  aTCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACGCACCACCGCCGAATAGGTa                                                                  <  1:229715/251‑1 (MQ=255)                                                                                                                                                       cACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACGCACCACCGCCGAATAGGTACATAg                                                             <  1:580692/251‑1 (MQ=255)                                                                                                                                                           gcgcTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAAt                                                                                                                                                                                                           <  1:431128/106‑1 (MQ=255)                                                                                                                                                           gcgcTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCaa                                                                                                                                                       >  1:415827/1‑158 (MQ=255)                                                                                                                                                           gcgcTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACGCCCCACCGCCGAATAGGTACAt                                                               <  1:368197/245‑1 (MQ=255)                                                                                                                                                           gcgcTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACGCACCACCGCCGAATAGGTACATAg                                                             <  1:370180/247‑1 (MQ=255)                                                                                                                                                           gcgcTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACGCACCACCGCCGAATAGGTACATAGTGc                                                          >  1:110842/1‑250 (MQ=255)                                                                                                                                                              cTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACGCACCACCGCCGAATAGGTACATAGTGCCTaa                                                      >  1:170560/1‑251 (MQ=255)                                                                                                                                                                gTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCaa                                                                                                                                                       >  1:160288/1‑153 (MQ=255)                                                                                                                                                                gTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACGCACCACCGCCGAATAGGTACATAGt                                                            <  1:131092/243‑1 (MQ=255)                                                                                                                                                                gTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACGCACCACCGCCGAATAGGTACATAGTGCCTAAgg                                                    >  1:760753/1‑251 (MQ=255)                                                                                                                                                                gTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACGCACCACCGCCGAATAGGTACATAGTGCCTAAgg                                                    >  1:534657/1‑251 (MQ=255)                                                                                                                                                                gTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACGCACCACCGCCGAATAGGTACATAGTGCCTAAgg                                                    >  1:223352/1‑251 (MQ=255)                                                                                                                                                                 tAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACGCACCACCGCCGAATAGGTACATAGTGCCTAAgg                                                    <  1:553168/250‑1 (MQ=255)                                                                                                                                                                 tAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACGCACCACCGCCGAATAGGTACATAGTGCCTAAgg                                                    <  1:193595/250‑1 (MQ=255)                                                                                                                                                                 tAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACGCACCACCGCCGAATAGGTACATAGTGCCTAAgg                                                    <  1:849333/250‑1 (MQ=255)                                                                                                                                                                 tAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACGCACCACCGCCGAATAGGTACATAGTGCCTAAgg                                                    <  1:183856/250‑1 (MQ=255)                                                                                                                                                                 tAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACGCACCACCGCCGAATAGGTACATAGTGCCTAAgg                                                    <  1:667323/250‑1 (MQ=255)                                                                                                                                                                 tAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACGCACCACCGCCGAATAGGTACATAGTGCCTAAGGt                                                   <  1:726638/251‑1 (MQ=255)                                                                                                                                                                    cGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACGCACCACCGCCGAATAGGTACATAGTGCCTAAGGTGAt                                                >  1:721739/1‑251 (MQ=255)                                                                                                                                                                          gTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAAcc                                                                                                                     <  1:542719/176‑1 (MQ=255)                                                                                                                                                                          gTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACGCACCACCGCCGAATAGGTACAt                                                               >  1:765995/1‑230 (MQ=255)                                                                                                                                                                          gTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACGCACCACCGCCGAATAGGTACATAGTGCCTAAGGTGATCACCaa                                          >  1:382109/1‑251 (MQ=255)                                                                                                                                                                            gCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACGCACCACCGCCGAATAGGTACATAGTGCCTAAGGTGATCACCAATg                                        >  1:308691/1‑251 (MQ=255)                                                                                                                                                                             cGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACGCACCACCGCCGAATAGGTACAt                                                               <  1:704174/227‑1 (MQ=255)                                                                                                                                                                               ttCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACGCACCACCGCCGAATAGGTACATAGTGCCTAAGGTGATCACCAATGGAt                                     <  1:649072/251‑1 (MQ=255)                                                                                                                                                                                 gaCTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATGGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACGCCAT‑AGGAAAGCCACCGATGCCTTCATAACCCGTCGCGCCGGCAAAACCGGACAGTAACAACGCACCACCGCCGAATAGGGACATAGTGCCCAAGGTGATCACCAATGGAt                                     >  1:32387/2‑249 (MQ=255)                                                                                                                                                                                 cACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACGCACCACCGCCGAATAGGTACATAGTGCCTAAGGTGATCACCAATGGAtt                                    >  1:777903/1‑250 (MQ=255)                                                                                                                                                                                            aTTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACGCACCACCGCCGAATAGGt                                                                   >  1:94645/1‑208 (MQ=255)                                                                                                                                                                                            aTTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACGCACCACCGCCGAATAGGTACAt                                                               <  1:188804/212‑1 (MQ=255)                                                                                                                                                                                            aTTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACGCACCACCGCCGAATAGGTACATAGTGCCTAAGGTGATCACCAATGGATTCACTTTGGTGTa                        >  1:114267/1‑251 (MQ=255)                                                                                                                                                                                               aaaaaCGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACGCACCACCGCCGAATAGGTACATAGTGCCTAAGGTGATCACCAATGGATTCACTTTGGTGTagag                     <  1:117609/251‑1 (MQ=255)                                                                                                                                                                                                  aaCGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACGCACCACAGCCGAATAGGTACATAGTGCCTAAGGTGATCACCAATGGATTCACTTTGGTGTAGAGaat                  <  1:156109/251‑1 (MQ=255)                                                                                                                                                                                                   aCGTGCTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACGCACCACCGCCGAATAGGTACATAGTGCCTAAGGTGATCACCAATGGATTCACTTTGGTGTAGAGaatc                 <  1:306917/251‑1 (MQ=255)                                                                                                                                                                                                        cTTCTGCCGATGGTGGTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACGCACCACCGCCGAATAGGTACATAGTGCCTAAgg                                                    <  1:855200/211‑1 (MQ=255)                                                                                                                                                                                                                  tggtggTTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACGCACCACCGCCGAATAGGTACATAGTGCCTAAGGTGATCACCAATGGATTCACTTTGGTGTAGAGAATCAATCCGGCATTAATc  <  1:402215/251‑1 (MQ=255)                                                                                                                                                                                                                       gtTTTGTGCATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCTATGCCTTCATACCCTGTCGCGCCGGCAAAACCGGACAGCAACAACGCACCACCGCCGAATAGGTACATAGTGCCTAAGGTGATCACCAATgg                                       <  1:443382/209‑1 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                                                                               GCCCAACACCACGGCGGTCAATACCGGCATCAGCAGGGAACTGCCTAAGTCGGAGCGGGCGGAACCGAAATAAGACACCAGCAACACCGCCACAAACGCAGCAATAACGCCAATAAAGGAATAAATCAATAATAATGTGCGACGAATCGGCACGGCGCTGTAACGGGAAGTGCGTTCACTTTGCCCGATTAAAAACGTGCTTCTGCCGATGGTGGTTTTGTGTATTAATAACCAAAAGATCAGCGTCATTAACAGAAAATACAGGATCGGCGTCGGAATGCCGAATAAGGTTTGATTGGCGAAGTCCAGCAACG‑CATCAGGAAAGCCACCGATGCCTTCATAACCTGTCGCGCCGGCAAAACCGGACAGCAACAACGCACCACCGCCGAATAGGTACATAGTGCCTAAGGTGATCACCAATGGATTCACTTTGGTGTAGAGAATCAATCCGGCATTAATC  >  1/1145525‑1145984

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑