Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,145,376	T→A	*307L (TAA→TTA)	unknown ←	Autoinducer 2 (AI‑2) ABC transporter, permease protein LsrD

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,145,376	0	T	A	100.0%	167.2 / NA	60	*307L (TAA→TTA)	unknown	Autoinducer 2 (AI‑2) ABC transporter, permease protein LsrD
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (27/33);  total (27/33)

GGATAAACCGAGTAACACGGAAAGTGCGGTCACAAAAAACGGCGTTTTTTGAACGTTGGCTTTGCCAACGGCCCCGTTAGGGGTGAACAAACGAGAGATCGAGTTTGTGAATAATTTTACACGCGTTTTCATATTCATTCTCCTCTTGAGTTGAATTTAATCTACTGCTGCCTGACGTTCGGTTCGCCGACGAACCCATTGCACAATAGCACCATAATGCAAACTGACGGACTTGCCGCTTTACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCATTTGCAATCCTTGTTGCAAATAACCGATGAGCATGGCGGCAATCGCCGTGCCGATAATGGAACCGGCTCCGCCATAGATATTGGCACCGCCCAACACCACGGCGGTCAATACCGGCATCAGCAGGGAACTGCCTAAGTCGGAGCGGGCGGAACCGAAATAAGACACCAGCAACACCGCCACAAA  >  1/1145135‑1145620                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     ggATAAACCGAGTAACACGGAAAGTGCGGTCACAAAAAACGGCGTTTTTTGAACGTTGGCTTTGCCAACGGCCCCGTTAGGGGTGAACAAACGAGAGATCGAGTTTGTGAATAATTTTACACGCGTTTTCATATTCATTCTCCTCTTGAGTTGAATTTAATCTACTGCTGCCTGACGTTCGGTTCGCCGACGAACCCATTGCACAATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGataa                                                                                                                                                                                                                                             >  1:285111/1‑251 (MQ=255)     aaaCCGAGTAACACGGAAAGTGCGGTCACAAAAAACGGCGTTTTTTGAACGTTGGCTTTGCCAACGGCCCCGTTAGGGGTGAACAAACGAGAGATCGAGTTTGTGAATAATTTTACACGCGTTTTCATATTCATTCTCCTCTTGAGTTGAATTTAATCTACTGCTGCCTGACGTTCGGTTCGCCGACGAACCCATTGCACAATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAAtaag                                                                                                                                                                                                                                         <  1:802412/251‑1 (MQ=255)      aaCCGAGTAACACGGAAAGTGCGGTCACAAAAAACGGCGTTTTTTGAACGTTGGCTTTGCCAACGGCCCCGTTAGGGGTGAACAAACGAGAGATCGAGTTTGTGAATAATTTTACACGCGTTTTCATATTCATTCTCCTCTTGAGTTGAATTTAATCTACTGCTGCCTGACGTTCGGTTCGCCGACGAACCCATTGCACAATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAAtaagt                                                                                                                                                                                                                                        <  1:415538/251‑1 (MQ=255)        ccGAGTAACACGGAAAGTGCGGTCACAAAAAACGGCGTTTTTTGAACGTTGGCTTTGCCAACGGCCCCGTTAGGGGTGAACAAACGAGAGATCGAGTTTGTGAATAATTTTACACGCGTTTTCATATTCATTCTCCTCTTGAGTTGAATTTAATCTACTGCTGCCTGACGTTCGGTTCGCCGACGAACCCATTGCACAATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAAtaagtaa                                                                                                                                                                                                                                      <  1:218355/251‑1 (MQ=255)           aGTAACACGGAAAGTGCGGTCACAAAAAACGGCGTTTTTTGAACGTTGGCTTTGCCAACGGCCCCGTTAGGGGTGAACAAACGAGAGATCGAGTTTGTGAATAATTTTACACGCGTTTTCATATTCATTCTCCTCTTGAGTTGAATTTAATCTACTGCTGCCTGACGTTCGGTTCGCCGACGAACCCATTGCACAATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAgg                                                                                                                                                                                                                                    <  1:645275/250‑1 (MQ=255)                cacGGAAAGTGCGGTCACAAAAAACGGCGTTTTTTGAACGTTGGCTTTGCCAACGGCCCCGTTAGGGGTGAACAAACGAGAGATCGAGTTTGTGAATAATTTTACACGCGTTTTCATATTCATTCTCCTCTTGAGTTGAATTTAATCTACTGCTGCCTGACGTTCGGTTCGCCGACGAACCCATTGCACAATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGAtaataa                                                                                                                                                                                                                                          <  1:320460/239‑1 (MQ=255)                cacGGAAAGTGCGGTCACAAAAAACGGCGTTTTTTGAACGTTGGCTTTGCCAACGGCCCCGTTAGGGGTGAACAAACGAGAGATCGAGTTTGTGAATAATTTTACACGCGTTTTCATATTCATTCTCCTCTTGAGTTGAATTTAATCTACTGCTGCCTGACGTTCGGTTCGCCGACGAACCCATTGCACAATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAAtaagt                                                                                                                                                                                                                                        >  1:176397/1‑241 (MQ=255)                    gAAAGTGCGGTCACAAAAAACGGCGTTTTTTGAACGTTGGCTTTGCCAACGGCCCCGTTAGGGGTGAACAAACGAGAGATCGAGTTTGTGAATAATTTTACACGCGTTTTCATATTCATTCTCCTCTTGAGTTGAATTTAATCTACTGCTGCCTGACGTTCGGTTCGCCGACGAACCCATTGCACAATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATaa                                                                                                                                                                                                                          >  1:395758/1‑251 (MQ=255)                         tGCGGTCACAAAAAACGGCGTTTTTTGAACGTTGGCTTTGCCAACGGCCCCGTTAGGGGTGAACAAACGAGAGATCGAGTTTGTGAATAATTTTACACGCGTTTTCATATTCATTCTCCTCTTGAGTTGAATTTAATCTACTGCTGCCTGACGTTCGGTTCGCCGACGAACCCATTGCACAATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGc                                                                                                                                                                                                                       <  1:50760/249‑1 (MQ=255)                         tGCGGTCACAAAAAACGGCGTTTTTTGAACGTTGGCTTTGCCAACGGCCCCGTTAGGGGTGAACAAACGAGAGATCGAGTTTGTGAATAATTTTACACGCGTTTTCATATTCATTCTCCTCTTGAGTTGAATTTAATCTACTGCTGCCTGACGTTCGGTTCGCCGACGAACCCATTGCACAATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGc                                                                                                                                                                                                                       <  1:679449/249‑1 (MQ=255)                                 caAAAAACGGCGTTTTTTGAACGTTGGCTTTGCCAACGGCCCCGTTAGGGGTGAACAAACGAGAGATCGAGTTTGTGAATAATTTTACACGCGTTTTCATATTCATTCTCCTCTTGAGTTGAATTTAATCTACTGCTGCCTGACGTTCGGTTCGCCGACGAACCCATTGCACAATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAAtt                                                                                                                                                                                                             <  1:836908/251‑1 (MQ=255)                                                    aaCGTTGGCTTTGCCAACGGCCCCGTTAGGGGTGAACAAACGAGAGATCGAGTTTGTGAATAATTTTACACGCGTTTTCATATTCATTCTCCTCTTGAGTTGAATTTAATCTACTGCTGCCTGACGTTCGGTTCGCCGACGAACCCATTGCACAATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATc                                                                                                                                                                                           <  1:373717/250‑1 (MQ=255)                                                            cTTTGCCAACGGCCCCGTTAGGGGTGAACAAACGAGAGATCGAGTTTGTGAATAATTTTACACGCGTTTTCATATTCATTCTCCTCTTGAGTTGAATTTAATCTACTGCTGCCTGACGTTCGGTTCGCCGACGAACCCATTGCACAATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCATTTGCaa                                                                                                                                                                                   <  1:738285/250‑1 (MQ=255)                                                              ttGCCAACGGCCCCGTTAGGGGTGAACAAACGAGAGATCGAGTTTGTGAATAATTTTACACGCGTTTTCATATTCATTCTCCTCTTGAGTTGAATTTAATCTACTGCTGCCTGACGTTCGGTTCGCCGACGAACCCATTGCACAATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCATTTGCAATcc                                                                                                                                                                                <  1:166796/251‑1 (MQ=255)                                                                          ccGTTAGGGGTGAACAAACGAGAGATCGAGTTTGTGAATAATTTTACACGCGTTTTCATATTCATTCTCCTCTTGAGTTGAATTTAATCTACTGCTGCCTGACGTTCGGTTCGCCGACGAACCCATTGCACAATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCAtt                                                                                                                                                                                        <  1:5542/231‑1 (MQ=255)                                                                           cGTTAGGGGTGAACAAACGAGAGATCGAGTTTGTGAATAATTTTACACGCGTTTTCATATTCATTCTCCTCTTGAGTTGAATTTAATCTACTGCTGCCTGACGTTCGGTTCGCCGACGAACCCATTGCACAATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAgg                                                                                                                                                                                                                                    <  1:609920/186‑1 (MQ=255)                                                                           cGTTAGGGGTGAACAAACGAGAGATCGAGTTTGTGAATAATTTTACACGCGTTTTCATATTCATTCTCCTCTTGAGTTGAATTTAATCTACTGCTGCCTGACGTTCGGTTCGCCGACGAACCCATTGCACAATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCATTTGCAATcc                                                                                                                                                                                >  1:563047/1‑238 (MQ=255)                                                                           cGTTAGGGGTGAACAAACGAGAGATCGAGTTTGTGAATAATTTTACACGCGTTTTCATATTCATTCTCCTCTTGAGTTGAATTTAATCTACTGCTGCCTGACGTTCGGTTCGCCGACGAACCCATTGCACAATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCATTTGCAATCCTTGTTGCAAATaa                                                                                                                                                                   >  1:628065/1‑251 (MQ=255)                                                                                ggggTGAACAAACGAGAGATCGAGTTTGTGAATAATTTTACACGCGTTTTCATATTCATTCTCCTCTTGAGTTGAATTTAATCTACTGCTGCCTGACGTTCGGTTCGCCGACGAACCCATTGCACAATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCATTTGCAATCCTTGTTGCAAATAACCGAt                                                                                                                                                              >  1:165625/1‑251 (MQ=255)                                                                                     gAACAAACGAGAGATCGAGTTTGTGAATAATTTTACACGCGTTTTCATATTCATTCTCCTCTTGAGTTGAATTTAATCTACTGCTGCCTGACGTTCGGTTCGCCGACGAACCCATTGCACAATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAAtaag                                                                                                                                                                                                                                         <  1:843184/171‑1 (MQ=255)                                                                                     gAACAAACGAGAGATCGAGTTTGTGAATAATTTTACACGCGTTTTCATATTCATTCTCCTCTTGAGTTGAATTTAATCTACTGCTGCCTGACGTTCGGTTCGCCGACGAACCCATTGCACAATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCATTTGCAATCCTTGTTGCAAATAACCGATGAGCa                                                                                                                                                         >  1:566363/1‑251 (MQ=255)                                                                                      aaCAAACGAGAGATCGAGTTTGTGAATAATTTTACACGCGTTTTCATATTCATTCTCCTCTTGAGTTGAATTTAATCTACTGCTGCCTGACGTTCGGTTCGCCGACGAACCCATTGCACAATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCATTTGCAATCCTTGTTGCAAATAACCGATGAGCAt                                                                                                                                                        <  1:485718/251‑1 (MQ=255)                                                                                                 gaTCGAGTTTGTGAATAATTTTACACGCGTTTTCATATTCATTCTCCTCTTGAGTTGAATTTAATCTACTGCTGCCTGACGTTCGGTTCGCCGACGAACCCATTGCACAATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCATTTGCAATCCTTGTTGCAAATAACCGATGAGCATGGCGGCAATCg                                                                                                                                             >  1:621656/1‑251 (MQ=255)                                                                                                                     ttACACGCGTTTTCATATTCATTCTCCTCTTGAGTTGAATTTAATCTACTGCTGCCTGACGTTCGGTTCGCCGACGAACCCATTGCACAATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATc                                                                                                                                                                                           <  1:163879/185‑1 (MQ=255)                                                                                                                          cgcgTTTTCATATTCATTCTCCTCTTGAGTTGAATTTAATCTACTGCTGCCTGACGTTCGGTTCGCCGACGAACCCATTGCACAATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGAAATACCGa                                                                                                                                                                                             >  1:453212/1‑178 (MQ=255)                                                                                                                                         attcTCCTCTTGAGTTGAATTTAATCTACTGCTGCCTGACGTTCGGTTCGCCGACGAACCCATTGCACAATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCATTTGCAATCCTTGTTGCAAATAACCGATGAGCATGGCGGCAATCGCCGTGCCGATAATGGAACCGGCTCCGCCATAGATATTGGc                                                                                                     <  1:752314/251‑1 (MQ=255)                                                                                                                                          ttcTCCTCTTGAGTTGAATTTAATCTACTGCTGCCTGACGTTCGGTTCGCCGACGAACCCATTGCACAATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCATTTGCAATCCTTGTTGCaa                                                                                                                                                                       <  1:157414/184‑1 (MQ=255)                                                                                                                                               ctctTGAGTTGAATTTAATCTACTGCTGCCTGACGTTCGGTTCGCCGACGAACCCATTGCACAATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCATTTGCAATCCTTGTTGCAAATAACCGATGAGCATGGCGGCAATCGCCGTGCCGATAATGGAACCGGCTCCGCCATAGATATTGGCACCGc                                                                                                >  1:310971/1‑250 (MQ=255)                                                                                                                                                       ttGAATTTAATCTACTGCTGCCTGACGTTCGGTTCGCCGACGAACCCATTGCACAATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACGGATCATTTGCAATCCTTGTTGCAAATAACCGATGAGCATGGCGGCAATCGCCGTGCCGATAATGGAACCGGCTCCGCCATAGATATTGGCACCGCCCAacac                                                                                         <  1:659013/249‑1 (MQ=255)                                                                                                                                                         gAATTTAATCTACTGCTGCCTGACGTTCGGTTCGCCGACGAACCCATTGCACAATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGc                                                                                                                                                                                                                       <  1:447468/121‑1 (MQ=255)                                                                                                                                                         gAATTTAATCTACTGCTGCCTGACGTTCGGTTCGCCGACGAACCCATTGCACAATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCATTTGCAATCCTTGTTGCAAATAACCGATGAGCATGGCGGCAATCGCCGTGCCGATAATGGAACCGGCTCCGCCATAGat                                                                                                           <  1:32496/229‑1 (MQ=255)                                                                                                                                                                aTCTACTGCTGCCTGACGTTCGGTTCGCCGACGAACCCATTGCACAATAGCACCATAATGCAAACTGACGGGCTTACCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCATTTGCAATCCTTGTTGc                                                                                                                                                                         <  1:76039/160‑1 (MQ=255)                                                                                                                                                                aTCTACTGCTGCCTGACGTTCGGTTCGCCGACGAACCCATTGCACAATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCATTTGCAATCCTTGTTGCAAATAACCGATGAGCATGGCGGCAATCGCCGTGCCGATAATGGAACCGGCTCCGCCATAGATATTGGCACCGCCCAACACCACGGCGGTCa                                                                              >  1:383856/1‑251 (MQ=255)                                                                                                                                                                                      gttcgCCGACGAACCCATTGCACAATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCATTTGCAATCCTTGTTGCAAATAACCGATGAGCATGGCGGCAATCGCCGTGCCGATAATGGAACCGGCTCCGCCATAGATATTGGCACCGCCCAACACCACGGCGGTCAATACCGGCATCAGCAGGGAAc                                                         <  1:196026/250‑1 (MQ=255)                                                                                                                                                                                             gacgaACCCATTGCACAATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCATTTGCAATCCTTGTTGCAAATAACCGATGAGCATGGCGGCAATCGCCGTGCCGATAATGGAACCGGCTCCGCCATAGATATTGGCACCGCCCAACACCACGGCGGTCAATAc                                                                          >  1:500997/1‑226 (MQ=255)                                                                                                                                                                                             gacgaACCCATTGCACAATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCATTTGCAATCCTTGTTGCAAATAACCGATGAGCATGGCGGCAATCGCCGTGCCGATAATGGAACCGGCTCCGCCATAGATATTGGCACCGCCCAACACCACGGCGGTCAATACCGGCATCAGCAGGGAAc                                                         <  1:415071/243‑1 (MQ=255)                                                                                                                                                                                               cgaACCCATTGCACAATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCATTTGCAATCCTTGTTGCAAATAACCGATGAGCATGGCGGCAATCGCCGTGCCGATAATGGAACCGGCTCCGCCATAGATATTGGCACCGCCCAACACCACGGCGGTCAATACCGGCATCAGCAGGGAACTGCCTAAGTc                                               <  1:553668/251‑1 (MQ=255)                                                                                                                                                                                                  aCCCATTGCACAATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCATTTGCAATCCTTGTTGCAAATAACCGATGAGCATGGCGGCAATCGCCGTGCCGATAATGGAACCGGCTCCGCCATAGATATTGGCACCGCCCAACACCACGGCGGTCAATACCGGCATCAGCAGGGAACTGCCTAAGTc                                               <  1:169546/248‑1 (MQ=255)                                                                                                                                                                                                   cccATTGCACAATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCATTTGCAATCCTTGTTGCAAATAACCGATGAGCATGGCGGCAATCGCCGTGCCGATAATGGAACCGGCTCCGCCATAGATATTGGCACCGCCCAACACCAcggc                                                                                   <  1:887089/211‑1 (MQ=255)                                                                                                                                                                                                    ccATTGCACAATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCATTTGCAATCCTTGTTGCAAATAACCGATGAGCATGGCGGCAATCGCCGTGCCGATAATGGAACCGGCTCCGCCATAGat                                                                                                           <  1:596868/186‑1 (MQ=255)                                                                                                                                                                                                         gCACAATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCATTTGCAATCCTTGTTGCAAATAACCGATGAGCATGGCGGCAATCGCCGTGCCGATAATGGAACCGGCTCCGCCATAg                                                                                                             <  1:287057/179‑1 (MQ=255)                                                                                                                                                                                                           acaATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCATTTGCAATCCTTGTTGCAAATAACCGATGAGCATGGCGGCAATCGCCGTGCCGATAATGGAACCGGCTCCGCCATAGATATTGGCACCGCCCAACACCACGGCGGTCAATACCGGCATCAGCAGGGAACTGCCTAAGTCGGAGCGGGCGGa                                   >  1:158402/1‑251 (MQ=255)                                                                                                                                                                                                            caATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCATTTGCAATCCTTGTTGCAAATAACCGATGAGCATGGCGGCAATCGCCGTGCCGATAATGGAACCGGCTCCGCCATAGATATTGGCACCGCCCAac                                                                                           >  1:673897/1‑194 (MQ=255)                                                                                                                                                                                                            caATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCATTTGCAATCCTTGTTGCAAATAACCGATGAGCATGGCGGCAATCGCCGTGCCGATAATGGAACCGGCTCCGCCATAGATATTGGCACCGCCCAACACCACGGCGGTCAATACCGGCATCAGCAGGGa                                                           >  1:816009/1‑226 (MQ=255)                                                                                                                                                                                                            caATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCATTTGCAATCCTTGTTGCAAATAACCGATGAGCATGGCGGCAATCGCCGTGCCGATAATGGAACCGGCTCCGCCATAGATATTGGCACCGCCCAACACCACGGCGGTCAATACCGGCATCAGCAGGGAACTGCCTAAGTCGGAGCGGGCGGaa                                  >  1:508825/1‑251 (MQ=255)                                                                                                                                                                                                            caATAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCATTTGCAATCCTTGTTGCAAATAACCGATGAGCATGGCGGCAATCGCCGTGCCGATAATGGAACCGGCTCCGCCATAGATATTGGCACCGCCCAACACCACGGCGGTCAATACCGGCATCAGCAGGGAACTGCCTAAGTCGGAGCGGGCGGaa                                  >  1:772542/1‑251 (MQ=255)                                                                                                                                                                                                              aTAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCATTTGCAATCCTTGTTGCAAATAACCGATGAGCATGGCGGCAATCGCCGTGCCGATAATGGAACCGGCTCCGCCATAGATATTGGCACCGCCCAACACCACGGCGGTCAATACCGGCATCAGCAGGGAACTGCCTAAGTc                                               >  1:791245/1‑236 (MQ=255)                                                                                                                                                                                                              aTAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCATTTGCAATCCTTGTTGCAAATAACCGATGAGCATGGCGGCAATCGCCGTGCCGATAATGGAACCGGCTCCGCCATAGATATTGGCACCGCCCAACACCACGGCGGTCAATACCGGCATCAGCAGGGAACTGCCTAAGTCGGAGCGGGCGGAAc                                 >  1:732822/1‑250 (MQ=255)                                                                                                                                                                                                               tAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAAtaagt                                                                                                                                                                                                                                        >  1:522270/1‑50 (MQ=255)                                                                                                                                                                                                               tAGCACCATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCATTTGCAATCCTTGTTGCAAATAACCGATGAGCATGGCGGCAATCGCCGTGCCGATAATGGAACCGGCTCCGCCATAGATATTGGCACCGCCCAACACCACGGCGGTCAATACCGGCATCAGCAGGGAACTGCCTAAGTCGGAGCGGGCGGAACCg                               >  1:801540/1‑251 (MQ=255)                                                                                                                                                                                                                    ccATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCa                                                                                                                                                                                          <  1:473677/91‑1 (MQ=255)                                                                                                                                                                                                                    ccATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCATTTGCAATcc                                                                                                                                                                                >  1:884479/1‑101 (MQ=255)                                                                                                                                                                                                                    ccATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCATTTGCAATCCTTGTTGCAAATAACCGATGAGCATGGCGGCAATCGCCGTGCCGATAATGGAACCGGCTCCGCCATAGATATTGGCACCGCCCAACACCACGGCGGTCAATACCGGCATCAGCAGGGAACTGCCTAAGTCGGAGCGGGCGGAACCGAAATa                          >  1:494440/1‑251 (MQ=255)                                                                                                                                                                                                                    ccATAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCATTTGCAATCCTTGTTGCAAATAACCGATGAGCATGGCGGCAATCGCCGTGCCGATAATGGAACCGGCTCCGCCATAGATATTGGCACCGCCCAACACCACGGCGGTCAATACCGGCATCAGCAGGGAACTGCCTAAGTCGGAGCGGGCGGAACCGAAATa                          >  1:239457/1‑251 (MQ=255)                                                                                                                                                                                                                      aTAATGCAAACTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCATTTGCAATCCTTGTTGCAAATAACCGATGAGCATGGCGGCAATCGCCGTGCCGATAATgg                                                                                                                              <  1:148138/149‑1 (MQ=255)                                                                                                                                                                                                                                cTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCATTTGCAATCCTTGTTGCAAATAACCGATGAGCATGGCGGCAATCGCCGTGCCGATAATGGAACCTGCTCCGCCATAGATATTGGCACCGCCCAACACCACGGCGGTCAATACCGGCATCAGCAGGGAACTGCCTAAGTCGGAGCGGGCGGAACCGAAATAAGACACCAGCaa              >  1:699009/1‑251 (MQ=255)                                                                                                                                                                                                                                cTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCATTTGCAATCCTTGTTGCAAATAACCGATGAGCATGGCGGCAATCGCCGTGCCGATAATGGAACCGGCTCCGCCATAGATATTGGCACCGCCCAACACCACGGCGGTCAATACCGGCATCAGCAGGGAACTGCCTAAGTc                                               >  1:609842/1‑218 (MQ=255)                                                                                                                                                                                                                                cTGACGGACTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCATTTGCAATCCTTGTTGCAAATAACCGATGAGCATGGCGGCAATCGCCGTGCCGATAATGGAACCGGCTCCGCCATAGATATTGGCACCGCCCAACACCACGGCGGTCAATACCGGCATCAGCAGGGAACTGCCTAAGTCGGAGCGGGCGGAACCGAAATAAGac                      <  1:313551/243‑1 (MQ=255)                                                                                                                                                                                                                                        cTTGCCGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCATTTGCAATCCTTGTTGCAAATAACCGATGAGCATGGCGGCAATCGCCGTGCCGATAATGGAACCGGCTCCGCCATAGATATTGGCACCGCCCAACACCACGGCGGTCAATACCGGCATCAGCAGGGAACTGCCTAAGTc                                               <  1:310506/210‑1 (MQ=255)                                                                                                                                                                                                                                            ccGCTTAACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCATTTGCAATCCTTGTTGCAAATAACCGATGAGCATGGCGGCAATCGCCGTGCCGATAATGGAACCGGCTCCGCCATAGATATTGGCACCGCCCAACACCACGGCGGTCAATACCGGCATCAGCAGGGAACTGCCTAAGTCGGAGCGGGCGGAACCGAAATAAGACACCAGCAACACCGCCACaaa  >  1:785389/1‑251 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                     GGATAAACCGAGTAACACGGAAAGTGCGGTCACAAAAAACGGCGTTTTTTGAACGTTGGCTTTGCCAACGGCCCCGTTAGGGGTGAACAAACGAGAGATCGAGTTTGTGAATAATTTTACACGCGTTTTCATATTCATTCTCCTCTTGAGTTGAATTTAATCTACTGCTGCCTGACGTTCGGTTCGCCGACGAACCCATTGCACAATAGCACCATAATGCAAACTGACGGACTTGCCGCTTTACACGATAATAAGTAAGGCGCCGGATAAAGCACCGGAAATTTCATTGGGAATACCGATCATTTGCAATCCTTGTTGCAAATAACCGATGAGCATGGCGGCAATCGCCGTGCCGATAATGGAACCGGCTCCGCCATAGATATTGGCACCGCCCAACACCACGGCGGTCAATACCGGCATCAGCAGGGAACTGCCTAAGTCGGAGCGGGCGGAACCGAAATAAGACACCAGCAACACCGCCACAAA  >  1/1145135‑1145620

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑