Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,141,917 T→G A32A (GCA→GCC)  unknown ← ATPase provides energy for both assembly of type IV secretion complex and secretion of T‑DNA complex (VirB4)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,141,9170TG100.0% 153.6 / NA 56A32A (GCA→GCC) unknownATPase provides energy for both assembly of type IV secretion complex and secretion of T‑DNA complex (VirB4)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (21/35);  total (21/35)

ATCGGAATCCAGTCATTAGCGGCTTCGCCGACTTCTTCGATAATTTGATGTAGGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCGGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCTACTTTGCGTGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTCCTCAACTACCGCGCGAGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTATTTGGTTGTTTCTC  >  1/1141672‑1142031
                                                                                                                                                                                                                                                     |                                                                                                                  
attggAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAAt                                                                                                               >  1:128730/4‑251 (MQ=255)
     aaTCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGt                                                                                                          <  1:839225/251‑1 (MQ=255)
       tCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTa                                                                                                        <  1:730905/251‑1 (MQ=255)
          aatCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGt                                                                                                     <  1:553512/249‑1 (MQ=255)
               ttAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACa                                                                                                <  1:178652/251‑1 (MQ=255)
                      cTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGgtt                                                                                         <  1:632697/251‑1 (MQ=255)
                               cttcttCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACAt                                                                                >  1:659899/1‑251 (MQ=255)
                               cttcttCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACAt                                                                                <  1:164158/251‑1 (MQ=255)
                                       aTAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAAc                                                                        <  1:871563/251‑1 (MQ=255)
                                         aaTTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACAt                                                                                >  1:633343/1‑241 (MQ=255)
                                          aTTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTAc                                                                     <  1:807295/251‑1 (MQ=255)
                                              ggttaaaagatgaagCGTTTTCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGgt                                                                                          >  1:257141/15‑226 (MQ=255)
                                              gATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACcgcg                                                                 >  1:672786/1‑251 (MQ=255)
                                                 gtaagATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACcgcgcgg                                                              >  1:322879/5‑250 (MQ=255)
                                                           tGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGc                                                    <  1:791288/251‑1 (MQ=255)
                                                            gCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTa                                                                      <  1:181386/232‑1 (MQ=255)
                                                                 aTCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACt                                                                       <  1:488331/226‑1 (MQ=255)
                                                                   ccTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCaa                                             >  1:456069/1‑250 (MQ=255)
                                                                     ttACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAacac                                          <  1:851997/251‑1 (MQ=255)
                                                                     ttACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAacac                                          <  1:854387/251‑1 (MQ=255)
                                                                     ttACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAacac                                          <  1:750601/251‑1 (MQ=255)
                                                                     ttACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAacac                                          <  1:739408/251‑1 (MQ=255)
                                                                     ttACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAacac                                          <  1:149394/251‑1 (MQ=255)
                                                                     ttACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAaca                                           >  1:599479/1‑250 (MQ=255)
                                                                             tGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCAC‑TAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCAt                                 <  1:195667/251‑1 (MQ=255)
                                                                                   aaTTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATAc                               <  1:534334/248‑1 (MQ=255)
                                                                                                  aCGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTAtaa             <  1:61885/251‑3 (MQ=255)
                                                                                                     cccTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAacac                                          <  1:178129/219‑1 (MQ=255)
                                                                                                     cccTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATAc                               <  1:584936/230‑1 (MQ=255)
                                                                                                     cccTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTAtaacaa          >  1:617088/1‑246 (MQ=255)
                                                                                                     cccTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTAtaacaa          <  1:753838/251‑6 (MQ=255)
                                                                                                     cccTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTAtaacaa          <  1:586823/251‑6 (MQ=255)
                                                                                                     cccTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTAtaacaa          <  1:551424/251‑6 (MQ=255)
                                                                                                     cccTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTAtaacaa          >  1:526553/1‑246 (MQ=255)
                                                                                                     cccTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTAtaacaa          <  1:743301/251‑6 (MQ=255)
                                                                                                     cccTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTAtaacaa          >  1:145040/1‑246 (MQ=255)
                                                                                                                             aGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATAc                               <  1:110964/206‑1 (MQ=255)
                                                                                                                              gCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTAtaacaattccatct  <  1:291606/234‑14 (MQ=255)
                                                                                                                                    gctgTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGt                                                                                                     >  1:358533/4‑129 (MQ=255)
                                                                                                                                       gTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTAtaacaa          >  1:119024/1‑212 (MQ=255)
                                                                                                                                                ccTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTc                       <  1:210560/195‑1 (MQ=255)
                                                                                                                                                ccTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTAtaacaa          >  1:419967/1‑203 (MQ=255)
                                                                                                                                                 cTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTAtaacaa          >  1:757303/1‑202 (MQ=255)
                                                                                                                                                          cTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATg                                                                                            <  1:811347/116‑1 (MQ=255)
                                                                                                                                                                  gTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAAc                                                                        <  1:885855/128‑1 (MQ=255)
                                                                                                                                                                  gTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCg                   <  1:634774/181‑1 (MQ=255)
                                                                                                                                                                       ggAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATAc                               <  1:627749/164‑1 (MQ=255)
                                                                                                                                                                              ggTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAacac                                          <  1:56101/146‑1 (MQ=255)
                                                                                                                                                                               gTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAacac                                          >  1:729603/1‑145 (MQ=255)
                                                                                                                                                                                                          gTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATAc                               <  1:516453/129‑1 (MQ=255)
                                                                                                                                                                                                                     aTTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTggg                                                     >  1:184333/1‑96 (MQ=255)
                                                                                                                                                                                                                      ttCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATAc                               <  1:351878/117‑1 (MQ=255)
                                                                                                                                                                                                                      ttCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACtt                             >  1:323269/1‑119 (MQ=255)
                                                                                                                                                                                                                      ttCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTAtaac            >  1:187834/1‑133 (MQ=255)
                                                                                                                                                                                                                              ccATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTAtaacaa          >  1:431875/1‑125 (MQ=255)
                                                                                                                                                                                                                               cATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTc                       >  1:32390/1‑116 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                  
ATCGGAATCCAGTCATTAGCGGCTTCGCCGACTTCTTCGATAATTTGATGTAGGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCGGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCTACTTTGCGTGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTCCTCAACTACCGCGCGAGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTATTTGGTTGTTTCTC  >  1/1141672‑1142031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑