Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	1	1,141,908	T→C	V35V (GTA→GTG)	unknown ←	ATPase provides energy for both assembly of type IV secretion complex and secretion of T‑DNA complex (VirB4)

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	1	1,141,908	0	T	C	100.0%	170.7 / NA	60	V35V (GTA→GTG)	unknown	ATPase provides energy for both assembly of type IV secretion complex and secretion of T‑DNA complex (VirB4)
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (24/36);  total (24/36)

GTTTATAAATCGGAATCCAGTCATTAGCGGCTTCGCCGACTTCTTCGATAATTTGATGTAGGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCGGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCTACTTTGCGTGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTCCTCAACTACCGCGCGAGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTATTTGGTTGTTTCTC  >  1/1141664‑1142031                                                                                                                                                                                                                                                     |                                                                                                                            gttTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTg                                                                                                                       >  1:376801/1‑251 (MQ=255)         attggAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAAt                                                                                                               >  1:128730/4‑251 (MQ=255)              aaTCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGt                                                                                                          <  1:839225/251‑1 (MQ=255)                tCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTa                                                                                                        <  1:730905/251‑1 (MQ=255)                   aatCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGt                                                                                                     <  1:553512/249‑1 (MQ=255)                        ttAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACa                                                                                                <  1:178652/251‑1 (MQ=255)                               cTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGgtt                                                                                         <  1:632697/251‑1 (MQ=255)                                        cttcttCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACAt                                                                                >  1:659899/1‑251 (MQ=255)                                        cttcttCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACAt                                                                                <  1:164158/251‑1 (MQ=255)                                           cttcAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCggg                                                                                                                   <  1:61151/213‑3 (MQ=255)                                                aTAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAAc                                                                        <  1:871563/251‑1 (MQ=255)                                                  aaTTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACAt                                                                                >  1:633343/1‑241 (MQ=255)                                                   aTTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTAc                                                                     <  1:807295/251‑1 (MQ=255)                                                       ggttaaaagatgaagCGTTTTCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGgt                                                                                          >  1:257141/15‑226 (MQ=255)                                                       gATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACcgcg                                                                 >  1:672786/1‑251 (MQ=255)                                                          gtaagATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACcgcgcgg                                                              >  1:322879/5‑250 (MQ=255)                                                                    tGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGc                                                    <  1:791288/251‑1 (MQ=255)                                                                     gCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTa                                                                      <  1:181386/232‑1 (MQ=255)                                                                          aTCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCgc                                                                                                                    >  1:512314/1‑180 (MQ=255)                                                                          aTCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACt                                                                       <  1:488331/226‑1 (MQ=255)                                                                            ccTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCaa                                             >  1:456069/1‑250 (MQ=255)                                                                              ttACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAacac                                          <  1:851997/251‑1 (MQ=255)                                                                              ttACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAacac                                          <  1:149394/251‑1 (MQ=255)                                                                              ttACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAacac                                          <  1:750601/251‑1 (MQ=255)                                                                              ttACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAacac                                          <  1:739408/251‑1 (MQ=255)                                                                              ttACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAacac                                          <  1:854387/251‑1 (MQ=255)                                                                              ttACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAaca                                           >  1:599479/1‑250 (MQ=255)                                                                                      tGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCAC‑TAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCAt                                 <  1:195667/251‑1 (MQ=255)                                                                                            aaTTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATAc                               <  1:534334/248‑1 (MQ=255)                                                                                                           aCGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTAtaa             <  1:61885/251‑3 (MQ=255)                                                                                                              cccTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAacac                                          <  1:178129/219‑1 (MQ=255)                                                                                                              cccTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATAc                               <  1:584936/230‑1 (MQ=255)                                                                                                              cccTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTAtaacaa          >  1:617088/1‑246 (MQ=255)                                                                                                              cccTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTAtaacaa          <  1:753838/251‑6 (MQ=255)                                                                                                              cccTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTAtaacaa          <  1:586823/251‑6 (MQ=255)                                                                                                              cccTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTAtaacaa          <  1:743301/251‑6 (MQ=255)                                                                                                              cccTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTAtaacaa          <  1:551424/251‑6 (MQ=255)                                                                                                              cccTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTAtaacaa          >  1:526553/1‑246 (MQ=255)                                                                                                              cccTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTAtaacaa          >  1:145040/1‑246 (MQ=255)                                                                                                                                      aGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATAc                               <  1:110964/206‑1 (MQ=255)                                                                                                                                       gCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTAtaacaattccatct  <  1:291606/234‑14 (MQ=255)                                                                                                                                            ggctgTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACttt                                                                                                                        >  1:872009/5‑111 (MQ=255)                                                                                                                                             gctgTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGt                                                                                                     >  1:358533/4‑129 (MQ=255)                                                                                                                                                gTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTAtaacaa          >  1:119024/1‑212 (MQ=255)                                                                                                                                                         ccTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTc                       <  1:210560/195‑1 (MQ=255)                                                                                                                                                         ccTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTAtaacaa          >  1:419967/1‑203 (MQ=255)                                                                                                                                                          cTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTAtaacaa          >  1:757303/1‑202 (MQ=255)                                                                                                                                                                   cTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATg                                                                                            <  1:811347/116‑1 (MQ=255)                                                                                                                                                                           gTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAAc                                                                        <  1:885855/128‑1 (MQ=255)                                                                                                                                                                           gTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCg                   <  1:634774/181‑1 (MQ=255)                                                                                                                                                                                ggAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATAc                               <  1:627749/164‑1 (MQ=255)                                                                                                                                                                                       ggTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAacac                                          <  1:56101/146‑1 (MQ=255)                                                                                                                                                                                        gTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAacac                                          >  1:729603/1‑145 (MQ=255)                                                                                                                                                                                                                   gTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATAc                               <  1:516453/129‑1 (MQ=255)                                                                                                                                                                                                                              aTTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTggg                                                     >  1:184333/1‑96 (MQ=255)                                                                                                                                                                                                                               ttCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATAc                               <  1:351878/117‑1 (MQ=255)                                                                                                                                                                                                                               ttCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACtt                             >  1:323269/1‑119 (MQ=255)                                                                                                                                                                                                                               ttCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTAtaac            >  1:187834/1‑133 (MQ=255)                                                                                                                                                                                                                                       ccATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTAtaacaa          >  1:431875/1‑125 (MQ=255)                                                                                                                                                                                                                                        cATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTc                       >  1:32390/1‑116 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                            GTTTATAAATCGGAATCCAGTCATTAGCGGCTTCGCCGACTTCTTCGATAATTTGATGTAGGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCGGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCTACTTTGCGTGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTCCTCAACTACCGCGCGAGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTATTTGGTTGTTTCTC  >  1/1141664‑1142031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑