Predicted mutation
evidence seq id position mutation annotation gene description
RA 1 1,141,806 G→T T69T (ACC→ACA)  unknown ← ATPase provides energy for both assembly of type IV secretion complex and secretion of T‑DNA complex (VirB4)

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*11,141,8060GT100.0% 203.8 / NA 75T69T (ACC→ACA) unknownATPase provides energy for both assembly of type IV secretion complex and secretion of T‑DNA complex (VirB4)
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base T (30/45);  total (30/45)

CAACCGGAATACCGCTGGCTTGTACCAATACCGGCATAGATAAAACAGCAATTAAAGTGAGTTTTTTAAACATTTTCATCACGTTCTCCTTATACACAACGTTGTTTATAAATCGGAATCCAGTCATTAGCGGCTTCGCCGACTTCTTCGATAATTTGATGTAGGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCGGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCTACTTTGCGTGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTCCTCAACTACCGCGCGAGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTATTTGGTTGTTTCTC  >  1/1141561‑1142031
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                 
ctacCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGttt                                                                                                                                                                                                                               >  1:149322/3‑250 (MQ=255)
 tacCGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCg                                                                                                                                                                                                                             <  1:842939/250‑1 (MQ=255)
   ccGGAATACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAg                                                                                                                                                                                                                           >  1:56737/1‑251 (MQ=255)
         tACCGCTAGCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCttt                                                                                                                                                                                                                     >  1:897662/1‑251 (MQ=255)
              ctagCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGc                                                                                                                                                                                                                 <  1:329409/247‑1 (MQ=255)
                agCTTGAACTAATGCCGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCa                                                                                                                                                                                                                >  1:72966/2‑249 (MQ=255)
                              ccGGTATAGACAAGACAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACggg                                                                                                                                                                                                 <  1:564945/250‑1 (MQ=255)
                                     agacaagaCAGCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGt                                                                                                                                                                                         >  1:187684/8‑251 (MQ=255)
                                               gCAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTa                                                                                                                                                                                                     >  1:35442/1‑229 (MQ=255)
                                                cAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGtgcgtaaagggctcgaaac                                                                                                                                                                                    <  1:750249/245‑19 (MQ=255)
                                                cAATTAAAGTGAGTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAAtt                                                                                                                                                                                  <  1:451251/247‑1 (MQ=255)
                                                            gTTTTTTAAAGATTTTCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCaaa                                                                                                                                                                  >  1:184391/1‑251 (MQ=255)
                                                                          ttCATTACGTTCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGa                                                                                                                                                    >  1:624481/1‑251 (MQ=255)
                                                                                   ttCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCGCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAAc                                                                                                                                           <  1:416843/251‑1 (MQ=255)
                                                                                   ttCTCCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAAc                                                                                                                                           <  1:880424/251‑1 (MQ=255)
                                                                                      tcCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTTACAGAATTTTTAAGTTTTCACGCCCTAATCCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAAc                                                                                                                                           <  1:284257/248‑1 (MQ=255)
                                                                                      tcCTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAAc                                                                                                                                           <  1:532746/248‑1 (MQ=255)
                                                                                       ccTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAAt                                                                                                                                                                 <  1:393963/225‑1 (MQ=255)
                                                                                       ccTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCAt                                                                                                                                        >  1:280177/1‑250 (MQ=255)
                                                                                       ccTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCAt                                                                                                                                        >  1:701495/1‑250 (MQ=255)
                                                                                       ccTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCAt                                                                                                                                        >  1:869660/1‑250 (MQ=255)
                                                                                       ccTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACACCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAAAAAGTACGGGaa                                                                                                                                                                                               <  1:62856/195‑1 (MQ=255)
                                                                                       ccTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTGCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGttt                                                                                                                                                                                                                               <  1:430370/163‑1 (MQ=255)
                                                                                        cTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTc                                                                                                                                                                                 <  1:696031/208‑1 (MQ=255)
                                                                                        cTTATACACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAAc                                                                                                                                           >  1:18203/1‑246 (MQ=255)
                                                                                           ataCACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCcaca                                                                                                                                   >  1:649929/1‑251 (MQ=255)
                                                                                            taCACAACGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACAt                                                                                                                                  <  1:144466/251‑1 (MQ=255)
                                                                                                 aaCGTTGTTTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATTCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATcc                                                                                                                             <  1:445174/251‑2 (MQ=255)
                                                                                                    gttgttTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGaa                                                                                                                                                                                                         <  1:466110/172‑1 (MQ=255)
                                                                                                    gttgttTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATcc                                                                                                                             <  1:86257/248‑2 (MQ=255)
                                                                                                       gttTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAAc                                                                                                                                           >  1:459239/1‑231 (MQ=255)
                                                                                                       gttTATAAATTGGAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTg                                                                                                                       >  1:376801/1‑251 (MQ=255)
                                                                                                               attggAATCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAAt                                                                                                               >  1:128730/4‑251 (MQ=255)
                                                                                                                    aaTCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGt                                                                                                          <  1:839225/251‑1 (MQ=255)
                                                                                                                      tCCAATCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTa                                                                                                        <  1:730905/251‑1 (MQ=255)
                                                                                                                         aatCATTAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGt                                                                                                     <  1:553512/249‑1 (MQ=255)
                                                                                                                            cATTAGCGGCTTCGCCAACTTCTTCTTTAATTTGATGTATGATTTCTGCGTTATCCTTACTGGTTGACAGTTTTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGTGCCCTTTACGCACTAAGAAAGtctg                                                                                                                                                                                                   >  1:708177/1‑151 (MQ=255)
                                                                                                                              ttAGCGGCTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACa                                                                                                <  1:178652/251‑1 (MQ=255)
                                                                                                                                     cTTCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGgtt                                                                                         <  1:632697/251‑1 (MQ=255)
                                                                                                                                       tCGCCAACTTCTTCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGaa                                                                                                                                             >  1:130807/1‑197 (MQ=255)
                                                                                                                                              cttcttCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACAt                                                                                <  1:164158/251‑1 (MQ=255)
                                                                                                                                              cttcttCAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACAt                                                                                >  1:659899/1‑251 (MQ=255)
                                                                                                                                                 cttcAATAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCggg                                                                                                                   <  1:61151/213‑3 (MQ=255)
                                                                                                                                                      aTAATTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAAc                                                                        <  1:871563/251‑1 (MQ=255)
                                                                                                                                                        aaTTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACAt                                                                                >  1:633343/1‑241 (MQ=255)
                                                                                                                                                         aTTTGATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTAc                                                                     <  1:807295/251‑1 (MQ=255)
                                                                                                                                                             ggttaaaagatgaagCGTTTTCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGgt                                                                                          >  1:257141/15‑226 (MQ=255)
                                                                                                                                                             gATGTAAGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACcgcg                                                                 >  1:672786/1‑251 (MQ=255)
                                                                                                                                                                gtaagATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACcgcgcgg                                                              >  1:322879/5‑250 (MQ=255)
                                                                                                                                                                          tGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGc                                                    <  1:791288/251‑1 (MQ=255)
                                                                                                                                                                           gCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTa                                                                      <  1:181386/232‑1 (MQ=255)
                                                                                                                                                                                aTCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCgc                                                                                                                    >  1:512314/1‑180 (MQ=255)
                                                                                                                                                                                aTCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACt                                                                       <  1:488331/226‑1 (MQ=255)
                                                                                                                                                                                  ccTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCaa                                             >  1:456069/1‑250 (MQ=255)
                                                                                                                                                                                    ttACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAacac                                          <  1:739408/251‑1 (MQ=255)
                                                                                                                                                                                    ttACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAacac                                          <  1:854387/251‑1 (MQ=255)
                                                                                                                                                                                    ttACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAacac                                          <  1:750601/251‑1 (MQ=255)
                                                                                                                                                                                    ttACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAacac                                          <  1:851997/251‑1 (MQ=255)
                                                                                                                                                                                    ttACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAacac                                          <  1:149394/251‑1 (MQ=255)
                                                                                                                                                                                    ttACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAaca                                           >  1:599479/1‑250 (MQ=255)
                                                                                                                                                                                            tGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCAC‑TAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCAt                                 <  1:195667/251‑1 (MQ=255)
                                                                                                                                                                                                  aaTTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATAc                               <  1:534334/248‑1 (MQ=255)
                                                                                                                                                                                                                 aCGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTAtaa             <  1:61885/251‑3 (MQ=255)
                                                                                                                                                                                                                   gCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATcc                                                                                                                             >  1:255172/1‑136 (MQ=255)
                                                                                                                                                                                                                    cccTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAacac                                          <  1:178129/219‑1 (MQ=255)
                                                                                                                                                                                                                    cccTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATAc                               <  1:584936/230‑1 (MQ=255)
                                                                                                                                                                                                                    cccTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTAtaacaa          >  1:526553/1‑246 (MQ=255)
                                                                                                                                                                                                                    cccTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTAtaacaa          >  1:145040/1‑246 (MQ=255)
                                                                                                                                                                                                                    cccTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTAtaacaa          <  1:753838/251‑6 (MQ=255)
                                                                                                                                                                                                                    cccTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTAtaacaa          <  1:743301/251‑6 (MQ=255)
                                                                                                                                                                                                                    cccTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTAtaacaa          >  1:617088/1‑246 (MQ=255)
                                                                                                                                                                                                                    cccTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTAtaacaa          <  1:586823/251‑6 (MQ=255)
                                                                                                                                                                                                                    cccTAAACCGCTTAAATCTAACTTAGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTAtaacaa          <  1:551424/251‑6 (MQ=255)
                                                                                                                                                                                                                                            aGCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATAc                               <  1:110964/206‑1 (MQ=255)
                                                                                                                                                                                                                                             gCTAAGGCTGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCCACTTTGCGGGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTGCTCAACTACCGCGCGGGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTAtaacaattccatct  <  1:291606/234‑14 (MQ=255)
                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                 
CAACCGGAATACCGCTGGCTTGTACCAATACCGGCATAGATAAAACAGCAATTAAAGTGAGTTTTTTAAACATTTTCATCACGTTCTCCTTATACACAACGTTGTTTATAAATCGGAATCCAGTCATTAGCGGCTTCGCCGACTTCTTCGATAATTTGATGTAGGATTTCTGCGTTATCCTTACTGGTTGACAGAATTTTTAAGTTTTCACGCCCTAAACCGCTTAAATCTAACTTAGCTAAGGCGGTTTCGAGCCCTTTACGCACTAAGAAAGTACGGGAATCCGGTTGTAATTCACGGATAATTTCAAATTGTTTTTCGCTGATTCCGAAACCATCCACATAATCTACTTTGCGTGCAATCGGGTTAGGTAAACAAATGGTTGTTTCACATTCCTCAACTACCGCGCGAGAAATTGGGCTTTTCAACACTTCATCCATACTTTGGGTCATCGGTATTTGGTTGTTTCTC  >  1/1141561‑1142031

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑